CN104982205B - Promote clematis Rouge Cardina cuttage breeding methods - Google Patents
Promote clematis Rouge Cardina cuttage breeding methods Download PDFInfo
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- CN104982205B CN104982205B CN201510410377.9A CN201510410377A CN104982205B CN 104982205 B CN104982205 B CN 104982205B CN 201510410377 A CN201510410377 A CN 201510410377A CN 104982205 B CN104982205 B CN 104982205B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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Abstract
Promote clematis Rouge Cardina cuttage breeding methods the invention discloses one kind, comprise the following steps：（1）Variety selection：（2）Cutting is gathered and handled；（3）Managed after inserting.The present invention is accelerates application of the Actions of Clematis Species on gardens, and meeting the market demand, there is provided certain reference.The a kind of of the present invention promotes clematis Rouge Cardina cuttage breeding methods, semi-lignified branch, IBA concentration are used for 2000mg/L and processing that the time of dipping is 15 seconds, survival rate, height of seedling and the number of blade is set all to have reached highest level, respectively 67.7%, 54cm and 10.66 piece.
Description
Technical field
The present invention relates to plant culture reproduction technique field, and in particular to promotes clematis Rouge Cardina cuttages numerous
Grow method.
Background technology
' Rouge Cardinal ' are Ranunculaceae Clematis (Clematis L.) plant to clematis kind.Clematis
Plant is world-renowned ornamental plant, about more than 300 is planted altogether, blazons the whole world, and China's germ plasm resource is enriched, and there are about 110 kinds, the whole nation
Various regions are distributed, especially in the majority with Central China and southwest distribution.Actions of Clematis Species is that one kind has gardening concurrently and medicinal dual
The valuable source plant of effectiveness, is mostly to climb up by holding on to liana, and its sight is strong, the laudatory title with " Climbing Plant queen ", state
It is outer many to the introducing and planting of clematis and breeding research, cultivate many new varieties.
This research is so that from the clematis cultivar of Poland's introduction, ' Rouge Cardinal ' are that material carries out cutting propagation
Experiment, kind ' Rouge Cardinal ' be wooden fallen leaves liana, first arrival in May at florescence by the end of July, nearly three months.Without sepal and flower
Valve, bract petal-shaped, Hua Jing about 17cm, pattern is gorgeous, petal rose, oval, single-lobe, and rhodo in the middle of petal, edge is light
It is pink；Filigree is faint yellow, flower pesticide oblong, purple, there is a vertical white line in the middle of flower pesticide.Corridor is can be applied in gardens, is stood
In the various vertical greenings such as post, it can also carry out potted plant moulding and view and admire.The country has no the research report to this kind, and this research is to add
Fast application of the Actions of Clematis Species on gardens, meeting the market demand, there is provided certain reference.
Yang Bijia etc. studies bastard clematis, and it is 400mg/L, processing as a result to show exogenous hormone methyl α-naphthyl acetate (NAA) concentration
Time 10min cuttage rooting situation is optimal.[Yang Bijia etc., bastard clematis cutting propagation Preliminary Study On Factors, Agriculture In Hubei Province section
Learn, in the April, 2014 the 7th phase of volume 53]
Zhang Qixiang etc. have studied influence of the exogenous hormone to blue cuttage root-taking more than clematis, as a result show IBA, 100mg/L,
Cuttage survival rate highest.[Zhang Qixiang etc., exogenous hormone is studied clematis rooting characteristic, northern gardening, the first phase in 2007]
Liu Bing etc. have studied the influence that different substrates, different agents are taken root to bastard indigo clematis epicormic branch cuttage, as a result show,
Cutting medium uses turfy soil：Perlite=1：1 and 200mg/LNAA+200mg/LIBA processing cuttings can improve rooting rate, root
Matter is good, and Root Distribution is uniform.[Guo Jun etc., the influence that different substrates, different agents are taken root to bastard indigo clematis epicormic branch cuttage, rather
Summer agriculture and forestry science and technology, the 4th phase in 2007]
Liu's equality have studied clematis common vetch peace Technique on Cuttage Propagation, as a result show：When NAA concentration is 200mg/L, processing
Between be 2h, peat and perlite proportioning be 1: 1 combination most useful for clematis cuttage root-taking, influence clematis microampere cuttage root-taking
Factor be matrix ＞ NAA processing time ＞ NAA concentration.[Liu's equality, the peace cuttage research of clematis common vetch, guangdong agricultural science
15th phase in 2011]
Above result of study shows, the requirement of the kind and kind of different clematis to cuttage environmental condition is different.
Conventional propagation method has seminal propagation, Plant Tissue Breeding and cutting propagation at present, but seminal propagation offspring is easy
Morph, it is impossible to keep the good characteristic of kind；Study on tissue culture cost is higher, and offspring easily degenerates；Therefore this research is adopted
With the method for cutting propagation.
The content of the invention
Goal of the invention：It is an object of the invention to provide a kind of promotion clematis Rouge Cardina cuttage breeding methods.
Technical scheme：In order to solve the above-mentioned technical problem, clematis Rouge Cardina are promoted the invention provides one kind
Cuttage breeding method, comprises the following steps：
(1) variety selection：Clip clematis lignifying and semi-lignified branch, it is desirable to which branch no disease and pests harm, stalwartness, bud are satisfied
Full, thickness is basically identical, cutting 5~6cm of length, per cutting containing 1 to sprouting；
(2) cutting collection and processing：Require that scissors is sharp when cutting cutting, cutting otch is smooth, and blade is entered during cuttage
Row shearing, stays 2-4 half vane, excessively makes cuttings dead to prevent rising, gathers morning and note moisturizing, first 500 after shearing
10min is soaked in times carbendazim solution, then clear water is rinsed, cleaned cutting is neat by the lower end discharge that is defined, every 30
A bundle is as one group of examination material, by every group of examination material base portion, in the heteroauxin solution for inserting various concentrations, quickly dipped several seconds, clear water
Compare, after having handled in insertion peat growing medium, often processing is repeated 3 times；
(3) managed after inserting：Sunshade is noted after cuttage and matrix moistening is kept, the sunshade net that rate is sheltered from heat or light in capping 75% after inserting,
Using the cuttage of full exposure mist bed, depending on spray time in seedbed is according to weather and moisture evaporation situation, high temperature season and
Fine day increases spray time, and seedbed is sprayed to prevent disease every 14d with 0.3% carbendazim, and sunshade is removed after 1 month
Net, pulls out weeds in seedbed in time, promotes stake seedling robust growth.
Wherein, above-mentioned cutting otch is flat nick, and lower cut is away from 3~4cm of bud, and upper cut is away from 1~2cm of bud.
Wherein, above-mentioned clematis uses semi-lignified branch.
Wherein, above-mentioned heteroauxin concentration is 2000mg/L.
Wherein, the time is dipped for 15 seconds.
Beneficial effect：The a kind of of the present invention promotes clematis Rouge Cardina cuttage breeding methods, using semi-lignified
The processing that branch, IBA concentration are 2000mg/L and the time of dipping is 15 seconds, makes survival rate, height of seedling and the number of blade all reach
Highest level, respectively 67.7%, 54cm and 10.66 piece.
Embodiment
Technical solution of the present invention is described in detail below, but protection scope of the present invention is not limited to the implementation
Example.
Embodiment：
1 test material and method
1.1st, test material
Test material is biennial clematis seedling of in the March, 2009 quoted from Poland.Test and carried out on May 16th, 2010,
Cuttings is picked up from 3 years raw branches.
1.2nd, method
1.2.1 cutting collection and processing
Clip clematis lignifying and semi-lignified branch, it is desirable to which branch no disease and pests harm, stalwartness, bud are full, thickness is basic
Unanimously, 5~6cm of cutting length, per cutting containing 1 to sprouting.Cutting otch is flat nick, and lower cut is away from 3~4cm of bud, upper cut
Away from 1~2cm of bud, require that scissors is sharp when cutting cutting, cutting otch is smooth.Blade is sheared during cuttage, 2-4 half is stayed
Blade, to prevent rising excessive, makes cuttings dead.Gather morning and note moisturizing.First soaked after shearing in 500 times of carbendazim solutions
10min is steeped, then clear water is rinsed, cleaned cutting is defined by lower end, and discharge is neat, and every 30 a bundles are used as one group of examination material.
By every group of examination material base portion, in IBA (heteroauxin) solution for inserting various concentrations, quickly dipped several seconds, clear water was compareed.Handle
Afterwards in insertion peat growing medium, often processing is repeated 3 times.Specifically handle as shown in table 1-1.
Table 1-1 handles factor and level
1.2.2 managed after inserting
Sunshade is noted after cuttage and matrix moistening is kept.The sunshade net that rate is sheltered from heat or light in capping 75% after inserting.Sprayed using full exposure
The cuttage of mist seedbed.Depending on spray time in seedbed is according to weather and moisture evaporation situation, high temperature season and fine day increase spraying
Time.Sprayed to prevent disease with 0.3% carbendazim every 14d in seedbed.Sunshade net is removed after 1 month, seedling is pulled out in time
Weeds in bed, promote stake seedling robust growth.
1.2.3 project is determined
September statistics survival rate, the number of blade, seedling growing height, the quantity of average terminal flower on the 2nd.
The statistical analysis of test data is carried out using Microsoft Excel, SPSS software.
2 interpretations of result
Influence of 2.1 different disposals to survival rate
The variance analysis explanation of table 2, the influence of factor branch and the reciprocation of IBA and time to survival rate is notable
's.Meanwhile, table 3 shows processing A2B2C3 namely semi-lignified branch, IBA concentration are 2000mg/L and the time of dipping is 15 seconds
When survival rate highest, survival rate to 67.7, next to that A2B1C2 and A2B1C1, survival rate has respectively reached 50% He
49.7%, and semi-lignified branch, IBA concentration be 1000mg/L, the time of dipping be respectively 5 and 10 seconds survival rate it is also higher.
In addition, table 3 also illustrate that, A1B1C3 survival rate is minimum, i.e., lignifying branch, IBA concentration are 1000mg/L, leaching
The time is dipped in for 15 seconds, its survival rate only has 18.7%.
Influence of 2.2 different disposals to height of seedling
As can be seen that influence of the factor branch to height of seedling is significant from the variance analysis of table 2.Can also be from table 3
Go out to handle A2B2C3 i.e. semi-lignified, IBA concentration be 2000mg/L, the time of dipping when being 15 seconds average seedling growth reach up to
54cm, next to that A2B1C2 and A2B2C1, i.e. semi-lignified branch, concentration 1000mg/L and 2000mg/L, dipping the time for 5 seconds
With 10 seconds, average height of seedling has all reached 52cm.
Influence of 2.3 different disposals to the number of blade
The variance analysis of table 2 illustrates that all factors all do not make significant difference to the number of blade.But table 3 illustrates that A2B2C3 is half wooden
The number of blade formed when change branch, IBA concentration are 2000mg/L and the time of dipping is 15 seconds is most, 10.66 pairs of average out to；Its
Secondary is A2B1C1, A2B1C2, A2B1C3, is 9.33 pairs of blades, illustrates that the time of dipping does not influence the formation of blade.
3 conclusions
In summary, A2B2C3, i.e. semi-lignified branch are handled, IBA concentration is 2000mg/L and the time of dipping is 15
The processing of second, makes survival rate, height of seedling and the number of blade all reach highest level.Therefore, processing A2B2C3 is optimization process.
The experimental factor analysis of variance table of table 2
Influence of the different disposal of table 3 to survival rate, height of seedling and the number of blade
Claims (1)
1. one kind promotes clematis Rouge Cardina cuttage breeding methods, it is characterised in that：Comprise the following steps：
（1）Variety selection：Clip clematis lignifying and semi-lignified branch, it is desirable to branch no disease and pests harm, stalwartness, bud be full,
Thickness is basically identical, cutting 5 ~ 6cm of length, per cutting containing 1 to sprouting；
（2）Cutting is gathered and handled：Require that scissors is sharp when cutting cutting, cutting otch is smooth, and blade is cut during cuttage
Cut, stay 2-4 half vane, excessively make cuttings dead to prevent rising, gather morning and note moisturizing, first more than 500 times after shearing
10min is soaked in bacterium spirit solution, then clear water is rinsed, cleaned cutting is defined discharge neatly by lower end, every 30 a bundles
As one group of examination material, by every group of examination material base portion, in the heteroauxin solution for inserting various concentrations, quickly dipped several seconds, clear water was done pair
According to after having handled in insertion peat growing medium, often processing is repeated 3 times；
（3）Managed after inserting：Sunshade is noted after cuttage and matrix moistening is kept, the sunshade net that rate is sheltered from heat or light in capping 75% after inserting, using complete
Depending on spray time in the cuttage of illumination mist bed, seedbed is according to weather and moisture evaporation situation, high temperature season and fine day increase
Plus spray time, seedbed is sprayed to prevent disease with 0.3% carbendazim every 14d, sunshade net is removed after 1 month, is pulled out in time
Except weeds in seedbed, promote stake seedling robust growth；The cutting otch is flat nick, and lower cut is away from 3 ~ 4cm of bud, and upper cut is away from bud
1~2cm；The clematis uses semi-lignified branch；The heteroauxin concentration is 2000mg/L, dips the time for 15 seconds.
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| CN201510410377.9A CN104982205B (en) | 2015-07-13 | 2015-07-13 | Promote clematis Rouge Cardina cuttage breeding methods |
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| CN104982205B true CN104982205B (en) | 2017-09-15 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106134836A (en) * | 2016-06-28 | 2016-11-23 | 广西南宁十分园生态农业科技有限公司 | A kind of Radix Clematidis cuttage vegetative propagation method |
| CN106613241A (en) * | 2016-12-23 | 2017-05-10 | 江苏农林职业技术学院 | Method for increasing survival rate of cut clematis florida strain contessa and promoting growth of seedlings |
| CN106613240A (en) * | 2016-12-23 | 2017-05-10 | 江苏农林职业技术学院 | Method of improving cutting survival rate of clematis variety Henry |
| CN108719031B (en) * | 2018-06-05 | 2020-12-22 | 江苏省中国科学院植物研究所 | Clematis water culture cutting rooting method |
| CN109089575A (en) * | 2018-08-13 | 2018-12-28 | 江苏农林职业技术学院 | The cottage method of clematis Poland spirit |
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