CN104885842A - Method for cutting and breeding Taxus baccata - Google Patents

Method for cutting and breeding Taxus baccata Download PDF

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CN104885842A
CN104885842A CN201510206623.9A CN201510206623A CN104885842A CN 104885842 A CN104885842 A CN 104885842A CN 201510206623 A CN201510206623 A CN 201510206623A CN 104885842 A CN104885842 A CN 104885842A
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王�忠
秦亚龙
徐敏
郑玉红
束晓春
宋向阳
彭峰
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Institute of Botany of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract

本发明公开了一种高效的欧洲红豆杉扦插繁育的方法:在五年生以上树龄的欧洲红豆杉植株顶部选取1~2年生半木质化枝条,用500 mg?L-1IBA或ABT处理,扦插至泥炭土、珍珠岩与河沙(体积3:1:1)的基质上进行生根培养,控制生根温度为20~28℃,空气湿度在75%~95%,当繁材开始长出新根时,在叶面喷施3~5次促生长营养液,促使其萌发嫩梢,最后对不同级别的快繁小苗实行分区栽植。该发明在设施栽培条件下用扦插方法繁殖欧洲红豆杉,不仅可以摆脱外界条件的影响,四季都能进行生产,而且可以节约育苗占地,降低生产成本。The invention discloses an efficient cutting propagation method of European yew: select 1 to 2-year-old semi-lignified branches from the top of the European yew plant over five years old, treat with 500 mg?L -1 IBA or ABT, and cut Carry out rooting culture on the substrate of peat soil, perlite and river sand (volume 3:1:1), control the rooting temperature at 20~28°C, and the air humidity at 75%~95%. At the same time, the growth-promoting nutrient solution is sprayed on the leaves 3 to 5 times to promote the germination of young shoots, and finally the fast-propagating seedlings of different levels are planted in different areas. The invention uses the cutting method to propagate the European yew under the condition of protected cultivation, which can not only get rid of the influence of external conditions, and can be produced in all seasons, but also can save the area occupied by seedlings and reduce the production cost.

Description

一种欧洲红豆杉扦插繁育的方法 A kind of method of European yew cutting propagation

技术领域 technical field

本发明涉及一种欧洲红豆杉扦插繁殖方法,属于植物的人工繁殖和栽培方法技术领域。 The invention relates to a European yew cutting propagation method, which belongs to the technical field of plant artificial propagation and cultivation methods.

背景技术 Background technique

欧洲红豆杉(Taxus baccata)是红豆杉科红豆杉属常绿乔木,原产欧洲、北非及西亚,我国庐山、南京等地有引种。欧洲红豆杉木材结构细腻且坚实耐腐;其种子出油率高且含大量蛋白质、脂肪、总糖等,可作工业用途;其枝、叶、根均可提取紫杉醇等生物原料,对卵巢癌、肺癌等癌症有较好的疗效;此外,欧洲红豆杉外观造型独特,四季常青的鲜艳绿色,种子成熟时鲜红饱满,鲜红色的假种皮与绿叶相映成趣,美观异常,是不可多得的高档绿化树种和盆栽植物材料。可以说欧洲红豆杉是集药用、材用、观赏于一身的重要资源植物和珍稀树种,具有极高的开发利用价值。 European yew ( Taxus baccata ) is an evergreen tree belonging to Taxaceae Taxus, native to Europe, North Africa and West Asia, and introduced in Lushan, Nanjing and other places in China. The wood structure of European yew is delicate and solid and resistant to decay; its seeds have high oil yield and contain a large amount of protein, fat, total sugar, etc., which can be used for industrial purposes; its branches, leaves, and roots can extract taxol and other biological materials, which are effective for ovarian cancer. , lung cancer and other cancers have a good curative effect; in addition, the European yew has a unique appearance, bright green evergreen throughout the year, bright red and plump when the seeds are mature, bright red arils and green leaves complement each other, beautiful and unusual, it is rare High-grade greening tree species and potted plant materials. It can be said that the European yew is an important resource plant and rare tree species integrating medicinal, timber and ornamental uses, and has extremely high development and utilization value.

欧洲红豆杉主要以“混生、复层、异龄”散生于自然界中,生长缓慢,自然更新困难。其天然更新方式有种子繁殖和无性萌芽繁殖两种,但由于该属、植物常散生于林中而导致的物种间隔,或花期不遇(雌雄异株、异花授粉),使其传粉受精受阻,最终形成的种子数量极有限;加之其种子假种皮味甘甜可食,鸟类、动物也取食,使残存种子数量更少。而且欧洲红豆杉的种子呈坚果状,种皮厚,处于深度休眠状态,自然状态下需要经过两冬一夏才能萌发。这些都制约了欧洲红豆杉的育种进程。 European yew is mainly scattered in nature with "mixed growth, multiple layers, and different ages". It grows slowly and is difficult to regenerate naturally. Its natural renewal methods include seed reproduction and asexual budding reproduction. However, due to the species interval caused by the genus and plants often scattered in the forest, or the lack of encounter during flowering (dioecious plants, cross-pollination), its pollination and fertilization are hindered. , the number of seeds finally formed is extremely limited; in addition, the arils of the seeds are sweet and edible, and birds and animals also eat them, so that the number of remaining seeds is even smaller. Moreover, the seeds of the European yew are nut-shaped, with thick seed coats, and are in a state of deep dormancy. Under the natural state, it takes two winters and one summer to germinate. These have restricted the breeding process of European yew.

发明内容 Contents of the invention

本发明的目的在于提供一种高效的欧洲红豆杉扦插繁育的方法,进行规模化生产以及以欧洲红豆杉快繁为基础的其他学科的研究工作的需要。 The purpose of the present invention is to provide an efficient method for the cutting propagation of the European yew to meet the needs of large-scale production and other disciplines based on the rapid propagation of the European yew.

本发明的技术方案是这样实现的,该种欧洲红豆杉快速繁育方法的主要特点是: The technical scheme of the present invention is achieved in that the main features of this kind of European yew rapid breeding method are:

A、插穗的选择与制作:在五年生以上树龄的欧洲红豆杉植株顶部选取1~2年生半木质化枝条,于清晨或傍晚剪材,剪平材料端口并保留顶芽。做到切面平滑,不伤芽,不破皮,不开裂。繁材长度为8~12cm,保留繁材上部2/3至3/4的针叶,并保留分叉处的“结点”。用枝剪剪下后,进行喷水保湿处理。 A. Selection and production of cuttings: Select 1 to 2-year-old semi-lignified branches from the top of the European yew plant over five years old, cut the material in the morning or evening, cut the material port and keep the terminal bud. Make the cut surface smooth, without hurting the buds, breaking the skin, or cracking. The length of the multicolored wood is 8~12cm, and the needles of the upper 2/3 to 3/4 of the multicolored wood are reserved, and the "nodes" at the forks are reserved. After cutting with branch scissors, carry out water spray moisturizing treatment.

B、插穗的处理:将剪好的插穗用500 mg•L-1IBA或ABT处理,处理部位为繁材下切口及基部枝段,浸泡时间以6 h为宜,浸泡的深度为2~3cm,如处理试剂的浓度增加至1000 mg•L-1,浸泡时间缩短至2 h,处理后立即进行扦插。 B. Treatment of cuttings: Treat the cut cuttings with 500 mg•L -1 IBA or ABT. The treated parts are the lower incisions and base branches of the multifarious material. The soaking time is preferably 6 hours, and the soaking depth is 2~3cm , if the concentration of the treatment reagent is increased to 1000 mg•L -1 , the soaking time is shortened to 2 h, and cuttings are carried out immediately after treatment.

C、扦插池与扦插基质:用透气性透水性较好的红砖铺成扦插池,底部铺一层以确保透水;以泥炭土、珍珠岩与河沙按体积3:1:1进行混合作为基质,扦插前一天浇水拌匀至能捏成拳而不散。 C. Cutting pool and cutting substrate: Pave the cutting pool with red bricks with good air permeability and water permeability, and lay a layer at the bottom to ensure water permeability; mix peat soil, perlite and river sand at a volume of 3:1:1 as the Substrate, water and mix well the day before the cuttings until they can be squeezed into fists without falling apart.

D、生根培养:繁材扦插时应直插,不得倒插、斜插,用小木棍在扦插基质预置插孔后再将繁材插入孔穴中,谨防插条切口与基质磨损受伤,繁材扦插后压实基质,务使基质与插条密接,扦插深度以3~4cm为宜,扦插密度以繁材枝叶不交叉重叠为准,一般400~600株/m2。材料扦插完毕后,立即开启微喷弥雾系统,并覆盖微膜小拱棚。控制生根温度为20~28℃,需要的空气湿度在细胞活化期、愈伤组织形成期、根原基产生期、根系形成期分别为90%~95%、85%~90%、80%~85%、75%~80%,在炼苗适应期的空气湿度应与大环境的空气湿度相近。通过实施系统调控,可根据繁材对湿度的需求变化而相应增减系统弥雾次数、弥雾时间,使苗床湿度保持在繁材生根的适宜范围内。 D. Rooting culture: When cutting the cuttings of the cuttings, they should be inserted straight, not upside down or obliquely. Use a small wooden stick to pre-set holes in the cutting matrix and then insert the cuttings into the holes. Be careful not to damage the cuttings and the matrix. After cutting, compact the substrate to ensure that the substrate and the cuttings are closely connected. The appropriate cutting depth is 3~4cm, and the cutting density is based on the fact that the branches and leaves of the flourishing materials do not cross and overlap, generally 400~600 plants/m 2 . After cutting the material, turn on the micro-spray and mist system immediately, and cover the small shed with micro-film. Control the rooting temperature at 20-28°C, and the required air humidity is 90%-95%, 85%-90%, 80%-85% during the cell activation period, callus formation period, root primordium production period, and root system formation period, respectively. %, 75%~80%, the air humidity in the seedling adaptation period should be similar to the air humidity in the general environment. Through the implementation of system regulation, the number of fogging times and fogging time of the system can be increased or decreased according to the change in the humidity demand of the growing materials, so that the humidity of the seedbed can be kept within the suitable range for the rooting of the growing materials.

E、施肥管理:当欧洲红豆杉繁材从开始长出新根至新根由乳白色转为红褐色这段时期,繁材叶面喷施3~5次促生长营养液(由常规营养液配方改进而来,如1/2MS营养液),可促使繁材在快繁苗床萌发嫩梢。 E. Fertilization management: During the period from the beginning of new roots of European yew until the new roots turn from milky white to reddish brown, spray 3 to 5 times of growth-promoting nutrient solution (improved by conventional nutrient solution formula) Come, such as 1/2MS nutrient solution), can promote the germination of young shoots in the rapid propagation seedbed.

F、快繁苗移植:快繁小苗应根据繁材类型、发根情况进行分级,不同级别的快繁小苗实行分区栽植。从起苗至移植全过程,要衔接进行,严防苗根风吹日晒。做到栽直、压实、不窝根,栽后及时浇(喷)灌“定根水”,使移栽苗根系与土壤密接。在具备快繁小苗移栽过渡大棚的育苗圃地,可周年从快繁苗床移栽快繁小苗。在非设施调控育苗圃地,以在10月下旬至11月下旬以及2月下旬至3月下旬移栽最佳。根据培育不同规格的苗木确定相应的密度,一般快繁小苗首次移栽过渡区,栽植密度以25cm×25cm为宜,亩栽8000株;培育中上规格成苗(包括采穗圃种苗)圃地,栽植密度以50cm×50cm为宜,亩栽2000株。在生产上,为提高苗圃地利用率,可实行计划密植栽植法,即采取一次定植、分批移植的办法来建立成苗圃地。如对亩栽8000株的圃地进行两次减半稀掘移植后,即成为亩栽2000株的稀植圃地。这样,在相同年限内,育苗圃地的苗木产出率远比考虑了大规格成苗长大而预留空间的苗圃高。 F. Transplanting of fast-propagating seedlings: Rapid-propagating seedlings should be graded according to the type of propagating material and rooting conditions, and fast-propagating seedlings of different levels should be planted in different areas. The whole process from seedling raising to transplanting should be carried out in succession to prevent the roots of the seedlings from being exposed to wind and sun. Make sure that the roots are planted straight, compacted, and not rooted. After planting, water (spray) the "fixed root water" in time to make the root system of the transplanted seedlings closely connected with the soil. In nurseries equipped with rapid-propagation seedling transplanting transition greenhouses, rapid-propagation seedlings can be transplanted from rapid-propagation seedbeds every year. In non-facility controlled nurseries, it is best to transplant from late October to late November and from late February to late March. Determine the corresponding density according to the cultivation of seedlings of different specifications. Generally, the fast-propagating seedlings are transplanted to the transition zone for the first time. The planting density is 25cm×25cm, and 8,000 plants are planted per mu; cultivate medium and upper-sized seedlings (including ear-picking nursery seedlings) nurseries The planting density is preferably 50cm×50cm, and 2000 plants are planted per mu. In terms of production, in order to improve the utilization rate of nursery land, the planned dense planting method can be implemented, that is, the method of one-time planting and batch transplantation is adopted to establish a nursery land. For example, after twice halving and transplanting a nursery with 8,000 plants per mu, it will become a sparsely planted nursery with 2,000 plants per mu. In this way, within the same period of time, the seedling output rate of the nursery nursery is far higher than that of the nursery that has reserved space for large-scale seedlings to grow up.

在设施栽培条件下采用扦插方法繁殖欧洲红豆杉,不仅可以摆脱外界条件的影响,四季都能进行生产,而且可以节约育苗占地,降低生产成本。通过扦插无性繁殖既保持了母本的优良性状,同时可形成大量后代植株且遗传性状稳定,在短期内形成大量优质种苗,进行规模化、工厂化生产。 Propagating Taxus chinensis by cuttings under protected cultivation conditions can not only get rid of the influence of external conditions, but also can be produced in all seasons, and can save the land occupied by seedlings and reduce production costs. Asexual reproduction by cuttings not only maintains the excellent traits of the female parent, but also can form a large number of offspring plants with stable genetic traits, and form a large number of high-quality seedlings in a short period of time for large-scale and industrial production.

具体实施方式 Detailed ways

下面结合欧洲红豆杉的快速繁殖的实例说明本发明的具体实施方式。 The specific embodiment of the present invention is described below in conjunction with the example of the rapid propagation of the European yew.

实例1欧洲红豆杉的扦插快繁 Example 1 Rapid propagation of Chinese yew by cuttings

1.2012年7月,在5年生欧洲红豆杉上取当年生枝条,平均50条/株,于清晨剪材,剪平材料端口并保留顶芽。繁材长度为8~12cm,保留繁材上部3/4的针叶,并保留分叉处的“结点”,共计获得2100条插穗。用2000 mg•L-1IBA浸泡插穗基部5-min,随即扦插至以泥炭土、珍珠岩与河沙按体积3:1:1混匀的基质上,开启微喷弥雾系统(每天喷雾3次,1min/次),保证湿度在75~85%,并适当遮荫(透光率70-80%)。 1. In July 2012, the current year's branches were taken from the 5-year-old European yew, with an average of 50 branches per plant, and the wood was cut in the early morning, and the end of the material was cut flat and the terminal buds were retained. The length of the multiplication material is 8-12cm, and the needle leaves of the upper 3/4 of the multiplication material are retained, and the "nodes" at the forks are retained, and a total of 2100 cuttings are obtained. Soak the base of the cuttings with 2000 mg·L -1 IBA for 5-min, then cut them on the substrate mixed with peat soil, perlite and river sand in a volume ratio of 3:1:1, and turn on the micro-spray mist system (spray 3 time, 1min/time), ensure the humidity is 75-85%, and properly shade (light transmittance 70-80%).

2.温室内保证通风,温度控制在25℃左右。自2012年8月起,利用随机抽样统计插穗的愈伤组织形成情况,保持扦插池无杂草生长,并剔除已枯萎的枝条。扦插2个月后随机抽样统计插穗的生根率,最终生根并成活的苗为1787棵,成活率为85%。 2. Ventilation is ensured in the greenhouse, and the temperature is controlled at about 25°C. Since August 2012, random sampling was used to count the callus formation of the cuttings, to keep the cutting pool free of weeds, and to remove the withered branches. After 2 months of cutting, the rooting rate of the cuttings was randomly sampled and counted. There were 1787 seedlings that finally took root and survived, and the survival rate was 85%.

实例2 快繁苗的移栽与出圃 Example 2 Transplanting and emergence of fast-propagating seedlings

1.自生根后2~3个月,即可将快繁苗进行移栽。从起苗至栽种的全过程,要衔接进行,防止苗和根受风吹日晒。做到栽直、压实、不窝根,栽后及时浇(喷)灌“定根水”,使移栽苗根系与土壤密接。栽植密度为25cm×25cm,亩栽8000株。移植后生长一年,对亩栽8000株的圃地再进行两次减半稀掘移植,成为亩栽2000株的稀植圃地。 1. The fast-propagating seedlings can be transplanted 2 to 3 months after rooting. The whole process from seedlings to planting should be carried out in succession to prevent seedlings and roots from being exposed to wind and sun. Make sure that the roots are planted straight, compacted, and not rooted. After planting, water (spray) the "fixed root water" in time to make the root system of the transplanted seedlings closely connected with the soil. The planting density is 25cm×25cm, and 8000 plants are planted per mu. After transplanting and growing for one year, the nursery with 8,000 plants per mu will be halved and transplanted twice to become a nursery with 2,000 plants per mu.

2.快繁小苗移栽的小规格种苗圃地采用管道喷灌系统,根据圃地土壤的水分状况适时、适量喷水。苗木生长初期,以施氮素化肥为主,后期以施磷、钾肥为主,施肥方式为水施,即施用波美2~3度的腐熟人粪尿或0.2%~0.3%浓度的液态化肥,一般在阴天或傍晚施用。除草掌握“除早、除小、除尽”的原则,除人工拔草、铲草外,采用5%精禾草克乳油等一些专杀禾本科杂草的选择性除草剂进行除草。在土壤板结的情况下及时松土,每年4~6次,松土深度以不伤苗木根系为准。经常检查种苗圃地,除去死苗、病苗、伤苗、弱苗,补植壮苗,使苗木分布均匀,生长整齐。 2. The small-sized seed nursery for transplanting fast-propagating seedlings adopts a pipeline sprinkler irrigation system, and sprays water in a timely and appropriate amount according to the moisture status of the nursery soil. In the early stage of seedling growth, nitrogen fertilizers are mainly applied, and in the later stage, phosphorus and potassium fertilizers are mainly applied. The fertilization method is water application, that is, the decomposed human excrement or 0.2%~0.3% concentration of Baume is applied. , generally applied on cloudy days or in the evening. Grasp the principle of "removing the early, removing the small, and eliminating all" in weeding. In addition to manual weeding and shoveling, use some selective herbicides such as 5% refined grass gram EC for weeding. In the case of soil compaction, loosen the soil in time, 4 to 6 times a year, and the depth of loosening should not damage the root system of the seedlings. Regularly check the seedling nursery, remove dead seedlings, sick seedlings, injured seedlings, and weak seedlings, and replant strong seedlings to make the seedlings evenly distributed and grow neatly.

3.1年生以内的快繁小苗可周年出圃,对于育苗圃地的大规格种苗以在10月下旬至11月下旬或2月下旬至3月下旬出圃最佳。保持根系完整,不损伤顶芽,不破损根皮,严禁用手拔苗。起苗深度视苗木根系生长状况而异,一般不得少于20cm。起苗后应在阴凉避风处,剔除劈根扯皮的、根系发育不健全的、缺损顶芽的和其它不合格的苗木,然后按苗木质量、大小规格标准进行分级。 3. The fast-propagating seedlings within 1 year old can be out of the nursery every year. For large-scale seedlings in the nursery nursery, it is best to be out of the nursery from late October to late November or from late February to late March. Keep the root system intact, do not damage the terminal buds, do not damage the root bark, and it is strictly forbidden to pull out the seedlings by hand. The seedling depth depends on the growth of the root system of the seedlings, and generally should not be less than 20cm. After the seedlings are raised, remove the seedlings that have split roots, those with undeveloped root systems, those with defective terminal buds, and other unqualified seedlings in a cool and sheltered place, and then grade them according to the quality and size standards of the seedlings.

Claims (1)

1. a method for european yew cutting propagation, is characterized in that:
The selection of A, cuttings and making: choose 1 ~ 2 year raw semi-lignified branch at the european yew plant top of 5 years above age of trees of life, cut material early morning or dusk, cut flat Material ports and retain terminal bud; Accomplish that tangent plane is level and smooth, do not hinder bud, not broken skin, do not ftracture; Numerous material length is 8 ~ 12cm, retains the needle on numerous material top 2/3 to 3/4, and retains " node " of crotch; After cutting with secateurs, carry out water spray moisturizing process;
The process of B, cuttings: by the cuttings sheared 500 mgL -1iBA or ABT process, treatment sites is numerous material lower cut and base portion branch section, and soak time is advisable with 6 h, and the degree of depth of immersion is 2 ~ 3cm, and the concentration as reagent treatment is increased to 1000 mgL -1, soak time foreshortens to 2 h, carries out cuttage immediately after process;
C, cuttage pond and cutting medium: be paved into cuttage pond with the good common brick of gas permeability water penetration, it is permeable to guarantee that bottom spreads one deck; Using peat soil, perlite and river sand by volume 3:1:1 carry out mixing as matrix, cuttage to be watered and to mix thoroughly to pinching into fist and do not fall apart the previous day;
D, culture of rootage: during numerous material cuttage, answer straight cutting, must not insert, oblique cutting, again numerous material is inserted in hole with small wooden after the preset jack of cutting medium, guard against cutting otch and matrix is worn and torn injured, compacting matrix after numerous material cuttage, matrix of make suring and cutting are touched, and the cuttage degree of depth is advisable with 3 ~ 4cm, cutting density with numerous material branches and leaves not juxtaposition be as the criterion, general 400 ~ 600 strains/m 2; After material cuttage, open micro-spray atomizing system immediately, and cover mocromembrane Small plastic shed; Control temperature of taking root is 20 ~ 28 DEG C, the air humidity needed is respectively 90% ~ 95%, 85% ~ 90%, 80% ~ 85%, 75% ~ 80% in cell activation phase, Callus formation phase, root restriction generation phase, root growth phase, should be close with the air humidity of overall situation in the air humidity of hardening laundering period; Regulated and controled by implementation system, can according to numerous material to the changes in demand of humidity corresponding increase and decrease system atomizing number of times, atomizing time, in seedbed humidity is remained on optimum range that numerous material takes root;
E, fertilizing management: when the numerous material of european yew from grow new root and transfer this period of bronzing to new root to by milky, numerous material foliage-spray 3 ~ 5 growth promotion nutrient solutions (are improved by conventional nutrient formula of liquid, as 1/2MS nutrient solution), numerous material can be impelled to sprout the tender tip at fast propagating seedling bed;
F, fast propagating seedling are transplanted: fast numerous seedling should carry out classification according to numerous material type, root of hair situation, and fast numerous seedling of different stage is carried out subregion and planted; From lifting to transplanting overall process, be connected and carry out, be strictly on guard against that shoot root is exposed to the weather; Accomplish to plant straight, compacting, not nest root, water (spray) after cultivation in time and fill with " normal root water ", transplanted seedling root system and soil are touched; On the Seedlings nursery ground possessing fast numerous little transplantation of seedlings transition booth, the anniversary fast numerous seedling can be transplanted from fast propagating seedling bed; On non-facilities regulation and control Seedlings nursery ground, to transplant best in late October to late November and late Febuary to late March; Nursery stock according to cultivating different size determines corresponding density, and general fast numerous seedling transplants transition region first, and planting density is advisable with 25cm × 25cm, and mu plants 8000 strains; Ground, upper specification seedling (comprising cutting orchard seedling) garden in cultivation, planting density is advisable with 50cm × 50cm, and mu plants 2000 strains; In production, be improve nursery lot availability, practicable plan dense planting planting method, namely take a field planting, the way of in batches transplanting to be created as nursery lot; After rare pick that reduces by half for twice as carried out with mu being planted to the garden of 8000 strains is transplanted, namely become the ground, thin planting garden that mu plants 2000 strains; Like this, in the identical time limit, the nursery of headspace is high more than considering that large gauge seedling is grown up for the nursery stock output capacity on Seedlings nursery ground.
CN201510206623.9A 2015-04-28 2015-04-28 Method for cutting and breeding Taxus baccata Pending CN104885842A (en)

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CN112753474A (en) * 2021-01-26 2021-05-07 荔波县黄江河国家湿地公园管理站 Taxus chinensis cutting cultivation method and application thereof
CN113519331A (en) * 2021-05-08 2021-10-22 山西红杉实业集团有限公司 Large-area large-scale taxus chinensis planting method
CN113519331B (en) * 2021-05-08 2024-01-26 山西红杉实业集团有限公司 Method for large-area large-scale planting of taxus chinensis

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Application publication date: 20150909