Method for promoting germination of lateral buds of succulent plants in Crassulaceae
Technical Field
The invention belongs to the technical field of plant cultivation, relates to a plant lateral bud germination technology, and particularly relates to a method for germinating lateral buds of a polyporus plant in Crassulaceae.
Background
Succulent plants are also called succulent plants and succulent plants, sometimes called succulent flowers in horticulture, and mainly refer to a group of plants which have developed parenchyma to store water and nutrients and appear thick and juicy in appearance, such as parts of vegetative organs of plants, such as stems, leaves or roots (a few species have both parts), Brown R d, effective radial absorbent tissue and specific properties of shade trees, Journal of abortive culture, 1990, 16 (6): 158-161, tang weng xiu, et al, introduction and cultivation experimental studies of succulent plants in Guilin plantations, northern horticulture, 2009 (1): 189-192. Commonly cultivated succulent plants include Crassulaceae, Cactaceae, Amygdalaceae, Euphorbiaceae, Liliaceae, Asclepiadaceae, Agavaceae, Apocynaceae, Portulacaceae, etc. The types of succulent plants in Crassulaceae are various and are subjected to intense heat treatment (Zu Bao Feng, the succulent plants in Crassulaceae are "hot-water" "," China flower and Garden (2008), 8: 10-11). Research on succulent plants is mainly focused on tissue culture (Gaoyue et al, tissue culture and rapid propagation of Maoylu, biological bulletin, 2010, 45 (6): 54-55), evaluation application (Wangcong et al, research on species screening of succulent plants cultivated in open fields in Xiamen and garden application thereof, subtropical plant science, 2009, 38 (4): 69-73), environmental benefits (Wuyufan et al, research on radiation reduction and humidification of succulent plants in business office environments, Shanghai agricultural science and technology, 2014, 4: 6-8), and the like. The common Crassulaceae 34 in the world belongs to more than 1500 species (China plant society, vol. 34(1) of China plant society, 1984: 72), and is generally propagated by cutting, sowing and tissue culture. Crassulaceae plant is CAM metabolic pathway plant, and has stomatal pore opening at night for absorbing CO2Is suitable for indoor planting; the succulent plant of Crassulaceae has the characteristics of various varieties, easy propagation, simple cultivation and management, shallow root system and the like, andThe composite material is relatively drought-resistant and cold-resistant, is suitable for family cultivation, roof greening and vertical greening, and has wide application prospects (baibin, broad prospects of stonecrop succulent plants, China floriculture, 005 th edition of 2013, 7 months and 25 days).
Succulent plants are usually bred in a head-cutting mode in production, on one hand, cut terminal buds can be used for cuttage breeding, and finished seedlings can be obtained more quickly than leaf cutting; on the other hand, the top advantages of the plants are removed by 'crop cutting', and the lateral buds of the parent plants can be germinated and used for propagation. However, the original ornamental value of the mother plant is damaged by 'head cutting', and the germination coefficient of lateral buds after head cutting is still limited.
Disclosure of Invention
In view of the defects of the prior art, the inventor researches the characteristics of vegetative growth and lateral bud germination of the polyporus plant of the Crassulaceae and creatively provides a method which is suitable for the polyporus plant of the Crassulaceae and can promote the lateral bud germination and improve the ornamental value of a mother plant through the technologies of fertilizing, picking leaves, spraying hormone and the like.
In order to achieve the purpose of the invention, the inventor finally obtains the following technical scheme through a large number of experimental researches:
A method for promoting lateral bud germination of a succulent plant in Crassulaceae comprises the following steps:
1) Selecting potted seedlings of a strong and disease-and-pest-free crassulaceae succulent plant which grows for more than two years, applying a granular organic fertilizer on the surface of a substrate in 2 months, wherein the application amount of the granular organic fertilizer is 0.2-0.35 kg/m calculated according to the bottom area of a flowerpot2And watering thoroughly after fertilizing.
2) after 4 weeks, picking 60% -70% of leaves of the stems of the plants from bottom to top, spraying 6-BA solution with the concentration of 800-1200 mg/L to the bare stems by using a sprayer, spraying 1 time per week for 4-6 times, wherein the spraying amount of each stem is 4-6 mL each time, and thus, the germination of lateral buds on the stems of the succulent plants in the Crassulaceae can be effectively promoted.
Preferably, the method for lateral bud germination of a succulent plant of the Crassulaceae family as described above, wherein the succulent plant comprises iridescent jade, Jones talocs, Sciazus, Bai-Liang, Samson beauty, and brilliant sunset.
Preferably, the method for germinating the lateral buds of the polyporus plants in the crassulaceae family is as described above, wherein the matrix used in the step 1) is a loose, air-permeable and water-draining mixed matrix such as yellow sand and imported peat soil.
Preferably, the method for germinating the lateral buds of the polyporus plants in the crassulaceae family as described above, wherein the preparation method of the granular organic fertilizer used in the step 1) comprises the following steps: mixing livestock and poultry manure and edible fungus waste materials according to the ratio of 3-4:1, then carrying out strip stacking, uniformly mixing RW ferment produced by Henan Biotechnology development Limited and edible fungus waste materials according to the ratio of 1:5-6, then scattering the mixture on the surface of the strip stacking, and stirring the strip stacking uniformly by a stirrer; fermenting for 15 days when the temperature reaches above 60 ℃, and then drying the moisture in the particles to the moisture content of below 20% through screening and granulation to obtain the granular organic fertilizer.
preferably, the method for germinating the lateral bud of the polyporus plant of the crassulaceae family as described above, wherein in the step 2), the method for removing the leaf is as follows: the leaves are pinched and gently shaken to the left and the right around the growing point of the leaves, so that the leaves are completely taken down without damaging the stems.
Preferably, the method for germinating the lateral bud of the polyporus plant of the crassulaceae family as described above, wherein in the step 2), the preparation method of the 6-BA solution is as follows: firstly, slowly dripping 3mol/L NaOH solution into the medicine powder until the medicine powder is completely dissolved, and then diluting the medicine powder into 800-1200 mg/L solution by using distilled water.
preferably, the method for lateral bud germination of the polyporus plants of the Crassulaceae family as described above, wherein the spraying method of the 6-BA solution in the step 2) is: and (3) spraying from top to bottom at a position which is about 4-6 cm away from the exposed stem, so that each surface of the exposed stem uniformly receives the 6-BA solution.
It should be noted that in the method for lateral bud germination of the polyporus umbiliciformis of the Crassulaceae family, the stem in the step 2) is the main stem of the polyporus umbiliciformis of the Crassulaceae family which grows for more than two years and is relatively stiff; the statistical lateral bud refers to the lateral bud which is germinated on the naked stem node, has changed into green color and has the diameter of more than 0.1 cm.
Preferably, the method for lateral bud germination of the polyporus plants in the crassulaceae family as described above, wherein the cultivation and management conditions of the potted seedlings in the steps 1) and 2) are as follows: the cultivation place is a thin film greenhouse in Wuhan city; watering by root soaking method, wherein watering is carried out 1 time per week in vigorous growth season, 1 time per month in dormancy period, and 1 time in half month in other periods; adopting 800 times of 50% carbendazim wettable powder, and irrigating roots or spraying leaf surfaces for 1 time in 14-30 days; shading by a shading net and cooling by a wet curtain in a sunny day of 6-9 months at 11: 00-15: 00; and opening the facility for ventilation for 8-12 h every day.
The method for promoting the germination of the lateral buds of the polyporus plants in the crassulaceae has the following advantages and remarkable progress:
1) The method for promoting the lateral buds of the polyporus plants in the Crassulaceae can effectively promote the lateral buds of the polyporus plants in the Crassulaceae to germinate, and the germination number of the lateral buds on plant stems in a test group can be increased to be multiple times of that of a blank group by fertilizing, picking leaves and spraying 6-BA solution.
2) The method for promoting the germination of the lateral buds of the polyporus plants in the crassulaceae family is used for fertilizing in 2 months, so that the nutritional requirements of the stock plant for vegetative growth and mass germination of the lateral buds when the potted seedlings in 3 months enter a vigorous growth period are met, and the stock plant grows robustly and the lateral buds grow and differentiate normally.
3) According to the method for promoting the lateral bud germination of the polyploid plant in the Crassulaceae, the granular organic fertilizer is only needed to be scattered on the surface of the pot soil during fertilization, holes are not needed to be dug to bury the fertilizer, the fertilizer and the matrix do not need to be uniformly mixed or paved on the bottom of the pot, and the operation is simple and convenient.
4) According to the method for promoting the germination of the lateral buds of the crassulaceae succulent plants, if the lateral buds germinated on the stock plants are cut off for cuttage, a large number of cuttings can be obtained in a short time, and the propagation coefficient of the crassulaceae succulent plants is greatly improved.
5) According to the method for promoting the germination of the lateral buds of the polyporus plants in the Crassulaceae, if the lateral buds germinated on the mother plant are not cut off for cuttage, the lateral buds are small, and the stems of the mother plant are almost covered by the small buds; after the lateral buds grow, the stock plant grows luxuriantly, the size of the stock plant is increased, a unique landscape is formed, and the ornamental value of the stock plant is greatly improved.
6) according to the method for promoting the germination of the lateral buds of the polyporus plant of the Crassulaceae, the picked leaves can be used for leaf cutting propagation, and the maximum utilization of resources is realized.
7) The method for promoting the germination of the lateral buds of the polypide plants in the Crassulaceae family only needs fertilization for 1 time, leaf picking for 1 time and spraying for 4-6 times, does not need other special conditions such as facilities and fields, is simple and convenient to operate, has low cost, and is beneficial to realizing large-scale production.
Detailed Description
The following are specific examples related to the present invention, and the technical solutions of the present invention will be further described, but the scope of the present invention is not limited to these examples. All changes, modifications and equivalents that do not depart from the spirit of the invention are intended to be included within the scope thereof.
Example 1 Effect of fertilization mode on lateral bud Germination and stock growth
In 2015 for 2 months, 6 crassulaceae succulent potted seedlings of rainbow jade, georgilloc, hazy moon, white, starry beauty, bright sunset and the like which grow consistently for more than two years, grow robustly and do not have diseases and insect pests are selected for carrying out the test.
The test site of the embodiment is a film greenhouse of Wuhan city agricultural institute forest fruit institute Wu lake base, the Wu lake base is located at 30 degrees 48 'north latitude, 114 degrees 91' east longitude, the annual average precipitation is 1000-1200mm, the annual average sunshine duration is 1540-2180h, the annual average temperature is 15.6 ℃, the annual frost period is 255 days, and the test site belongs to subtropical monsoon climate. The test greenhouse seedbed is in the north-south direction, and the height from the ground is 1 m. The greenhouse adopts 50 percent sunshade net for shading, ventilation by a ventilation fan and wet curtain for cooling, and has no heating and light supplementing facilities, the same as the following steps.
the method comprises the steps of respectively arranging a test group 1, a test group 2 and a blank group for 6 varieties of succulent plants, wherein each group comprises 30 pots, a flowerpot is a plastic square cup with the side length of 8cm, the cup height is 12cm, a matrix is a mixed matrix (the mass is about 0.2kg) of yellow sand and peat soil according to a ratio of 1:1, the test group 1 is applied with a granular organic fertilizer (500 kg of cow dung, 300kg of chicken manure and 200kg of edible fungus waste are piled in a strip mode, the width is 2m, the height is 1m, and the length is not limited), 100g of a RW ferment agent (produced by Henan Mingyuan biotechnology development Limited company) and the edible fungus waste are expanded in volume according to a ratio of 1:5, the ferment agent is uniformly mixed and then spread on the surface of the strip pile, a stirrer is used for uniformly stirring the strip pile, the temperature is higher than 60 ℃ and is fermented for 15 days, then sieving and granulation are carried out, the moisture in the granules is dried to be lower than 20%, and then the granules are packaged to, the fertilizing method is that the fertilizer is directly spread on the surface of the substrate. The test group 2 is applied with more flowers than No. 1 and 0.2g, and the fertilizing method is that 2 holes are uniformly dug at a position slightly far away from the root, fertilizer is applied in the holes, and soil is covered; the blank group was not fertilized. All 3 groups are watered thoroughly by a root soaking method. After 4 weeks, picking 60% -70% leaves of the stems of the plants of the blank group and the test group from bottom to top, spraying 6-BA solution with the concentration of 1000mg/L to the bare stems by a sprayer, spraying 1 time per week, and spraying 5 times totally, wherein the spraying amount of each stem is about 5mL each time. And respectively counting the growth conditions of 6 potted seedling stock plants and the number of lateral buds (the color is changed into green and the diameter is more than 0.1 cm) germinated on each bare stem when the distance between the stock plants and the first spraying is 2 months and 6 months away from the end of the first spraying, and taking an average value of the counting results, and carrying out the following steps.
And (3) test results: after 2 months of hormone spraying for the first time, the number of lateral buds of the test group 1 is equivalent to that of the blank group, but the number of new buds germinated in the test group 1 is slightly larger; the lateral buds of the test group 2 germinate fastest and the plants grow fast. After 6 months, the germination number of lateral buds of the test group 1 reaches 1.3 (rainbow jade) -1.7 (dimly moon) times of that of the blank group, and the growth vigor of plants and buds is good; the germination number of lateral buds in the test group 2 reaches 1.1 (rainbow jade) -1.8 (clouded moon) times of that in the blank group, but leaves and buds are small, internodes are long, the number of leaves is small, partial stems are prone to lodging, and the ornamental value is poor. See tables 1-6.
TABLE 1 Effect of fertilization on the number of lateral buds germinated in Yu-hong and the growth of the parent plant
TABLE 2 Effect of fertilization on the number of lateral buds germinated in Joey staroc and the growth of the parent plant
TABLE 3 influence of fertilization on lateral bud germination and parent plant growth in Hypsizygus months
TABLE 4 influence of fertilization on the germination count of the lateral bud of California, the growth of the parent plant
TABLE 5 Effect of fertilization on the lateral bud Germination number of the Sauropus androgynus and the growth of the stock plant
TABLE 6 influence of fertilization on the number of lateral buds sprouting and parent plant growth
Example 2 Effect of synergistic effects of fertilizing, leaf picking and 6-BA spraying on lateral bud germination number and parent plant growth
Selecting succulent plants of 6 varieties such as rainbow jade, geousistaroc, hazy moon, white, celestial beauty, bright sunset and the like which grow robustly and without diseases and insect pests for more than two years in 2 months of 2015, respectively setting a test group 1, a test group 2, a test group 3, a test group 4 and a blank group, wherein each group comprises 30 pots, each pot is a plastic square cup with the side length of 8cm and the cup height of 12cm, the matrix is a mixed matrix of yellow sand and peat soil according to a ratio of 1:1 (the mass of the pot soil is about 0.2kg), the test group 1 is fertilized according to the method of the test group 1 in the embodiment 1, and after 4 weeks, 60-70% of leaves of the stem of the plant are picked from bottom to top; the test group 2 is applied with fertilizer without picking leaves; test group 3 did not fertilize but picked leaves; the test group 4 is applied with fertilizer without picking leaves but cutting heads (the cutting head method is that the part between the 3 rd layer and the 4 th layer of leaves from the top of the stem is cut off, and the cut head can be used for cutting propagation); the blank group was not fertilized and the leaves were not removed. After picking the leaves (not picking the leaves), spraying 6-BA solution with the concentration of 1000mg/L to the naked stems of the test group 1, the test group 2, the test group 3 and the blank group for 5 times every week, wherein the spraying amount of each stem is about 5mL each time; and (4) spraying distilled water to the test group and the blank group. And respectively counting the growth conditions of 6 potted seedling mother plants and the number of lateral buds (the color is changed into green and the diameter is more than 0.1 cm) germinated on each bare stem when the distance between the first spraying and the second spraying is 2 months and 6 months.
And (3) test results: the germination numbers of the lateral buds of the test group 1 and the test group 3 are higher than those of the blank group at 2 months and 6 months, the time difference between the germination numbers of the lateral buds of the test group 1 and the test group 3 is not large at 2 months, the test group 1 is obviously higher than that of the test group 3 at 6 months, and the growth states of plants, leaves and buds of the test group 1 are better than that of the test group 3; the lateral bud germination number of the test group 2 is also higher than that of the blank group, but the difference is far smaller than that of the test groups 1 and 3 and the blank group, and the plants grow robustly; the test group 4 had a higher number of lateral buds sprouting at 2 months and 6 months than the blank group and the test group 2, and a lower number of lateral buds sprouting than the test group 1 and the test group 3, and had large and strong buds.
All test groups have more lateral buds sprouting on stems compared with the blank group, and the test group 1 has the best lateral bud sprouting number and growth vigor; at 2 months, the lateral buds of 6 Crassulaceae succulent plant test groups 1 such as rainbow jade, Jones talock, Scizy moon, white, Qingxing beauty, and bright sunset are 8.3, 8.0, 3.3, 6.9, 14.0, and 2.0 times of the blank group respectively; when 6 months later, the lateral buds further germinate and grow, the number of the lateral buds of the test group 1 of 6 crassulaceae succulent plants is 9.3, 6.7, 3.5, 3.9, 5.1 and 7.3 times of that of the blank group, which is shown in the table 7-12.
TABLE 7 influence of synergistic effect of fertilization and leaf picking on germination number of lateral bud of iris jade and growth of parent plant
TABLE 8 influence of synergistic effect of fertilization and leaf picking on the number of lateral buds sprouting and the growth of the stock plant
TABLE 9 influence of synergistic effects of fertilization and leaf picking on the germination number of lateral buds of dimly moon and the growth of mother plants
TABLE 10 influence of synergistic effects of fertilization and leaf picking on germination number of lateral buds of Baili and growth of parent plant
TABLE 11 influence of synergistic effects of fertilization and leaf picking on the germination number of lateral buds and the growth of parent plant of Gynura sinica
TABLE 12 influence of synergistic effects of fertilization and leaf picking on germination number of bright-day lateral buds and growth of parent plant
Example 36 Effect of BA concentration on lateral shoot Germination, mother plant growth
In 2 months 2014, 6 varieties of potted seedlings of crassulaceae succulents, such as jasper, geousitache, hazy moon, white, starry beauty, bright sunset and the like which grow consistently and strongly without diseases and insect pests for more than two years are selected, test groups 1-7 and blank groups are set, 30 pots are applied to each group, and the leaves are picked according to the method of the test group 1 in the embodiment 1.
Spraying 6-BA solution to the bare stems of the test groups by using a sprayer, wherein the concentrations of the test groups 1-7 are respectively 10, 50, 100, 500, 1000, 2000 and 3000mg/L, spraying distilled water to the blank groups for 1 time every week and 5 times in total, and the spraying amount of each stem is about 5 mL. And respectively counting the growth conditions of the 6 potted seedling mother plants and the number of lateral buds sprouting on each bare stem at a distance of 2 months and 6 months from the end of the first spraying.
And (3) test results: blank groups of 6 test varieties and test groups 1-5 (the concentration of 6-BA is 10-1000mg/L) have strong plants and normal lateral bud development in 2 months and 6 months, and the number of newly germinated lateral buds is increased along with the increase of the concentration of 6-BA; when the concentration is increased to 2000mg/L and 3000mg/L, 6 varieties have phytotoxicity with different degrees, the symptoms of the phytotoxicity are obvious at 2 months, and the plants are endangered to die or die at 6 months, which is shown in tables 13-18.
TABLE 136-BA concentration Effect on the number and growth of lateral buds of Rainbow jade
TABLE 146-influence of BA concentration on the number and growth vigor of lateral Cholesta buds
Influence of Table 156-BA concentration on the number and growth of Scutellaria sporophore
TABLE 166-BA concentration influence on the number and growth of lateral buds of Baili
TABLE 176-BA concentration influence on the lateral bud number and growth vigor of the Qingxing American
TABLE 186-BA concentration influence on the number and growth vigor of lateral buds on bright sunspot
Example 46 Effect of BA spray frequency on lateral bud Germination number
In 2015 for 2 months, 120 pots of 6 varieties of potted seedlings of crassulaceae succulents with consistent growth vigor, robust growth and no plant diseases and insect pests for more than two years are selected, test groups 1-3 and blank groups are respectively arranged, 30 pots in each group are respectively fertilized and subjected to leaf picking according to the method of the test group 1 in the embodiment 1.
Spraying 1000 mg/L6-BA solution to the bare stems of the test groups by using a sprayer, spraying 1 time per week, spraying 3 times, 5 times and 8 times respectively to the test groups 1 to 3, wherein the spraying amount of each stem is about 5mL each time, and spraying no to the blank groups. And respectively counting the number of lateral buds sprouting on the bare stems of the 6 potted seedlings at a distance of 2 months and 6 months from the end of the first spraying.
And (3) test results: the number of lateral buds germinated in 6 months in the test group and the blank group is higher than 2 months; the lateral bud numbers of the test group 2 are all higher than that of the test group 1, and the test group 3 is higher than that of the test group 2 (except for the Samsung Americans), but the increase of the test group 3 is not large compared with the test group 2. From the viewpoint of remarkable effect, low cost and simple operation, 5 times of spraying was the optimum number of treatments, as shown in table 19.
TABLE 196 Effect of spray frequency of BA on lateral bud Germination of Crassulaceae succulent plants