CN114190251B - Induction method for promoting proliferation of babysbreath lateral buds - Google Patents
Induction method for promoting proliferation of babysbreath lateral buds Download PDFInfo
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- CN114190251B CN114190251B CN202111474821.5A CN202111474821A CN114190251B CN 114190251 B CN114190251 B CN 114190251B CN 202111474821 A CN202111474821 A CN 202111474821A CN 114190251 B CN114190251 B CN 114190251B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses an induction method for promoting proliferation of a lateral bud of gypsophila paniculata, which comprises the following steps: selecting tissue culture seedlings which are transplanted into the plug tray and grow for 40 days and have uniform characters; topping the leaves after the gypsophila paniculata is transplanted to soil for 30 days, and then spraying plant cell division hormone 6-BA on the leaves; respectively spraying plant cell division hormone 6-BA after topping of the gypsophila paniculata and 7 days after topping, and respectively counting the germination number of new lateral branches after the gypsophila paniculata grows for 7 days and 14 days; when the growth of the babysbreath branches sprayed with the plant cell division hormone 6-BA reaches 70-100 cm and the flowering rate reaches 75%, the babysbreath branches can be harvested. According to the invention, by spraying the plant cytokinin 6-BA on the leaf surfaces of the gypsophila paniculata branches, a large amount of new buds can be induced, the actual production economic value of the gypsophila paniculata is improved, the technical guarantee is provided for the subsequent seedling breeding, and the foundation is laid for the development of the gypsophila paniculata cultivation industry.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to an induction method for promoting proliferation of a babysbreath bud.
Background
Gypsophila paniculata (also known as nephelina, caryophyllaceae, dianthus); the plant is a perennial herbaceous plant, the height of the plant is 0.5-1 meter, the diameter of the crown is about 0.5 meter, the growth speed is high, the branching capability is strong, and the plant shape is short and compact; the method is applied to garden isolation belts, courtyard cultivation, fresh cut flowers and the like, and has wide market prospect. The Yunnan has various climatic environments and rich biological resources, and can realize the cultivation of gypsophila all-season cultivation. At present, in actual cultivation, due to factors such as lack of variety characteristics and experience of growers, the gypsophila paniculata bud division rate is low, the lateral branches grow less, the flowering rate is low, and the economic benefit of the growers is influenced. Therefore, there is a need for an induction method for promoting the proliferation of the lateral bud of gypsophila paniculata.
Disclosure of Invention
Aiming at the problems, the invention provides an induction method for promoting the proliferation of the lateral bud of the gypsophila paniculata, and the germination rate and the yield of the lateral branch of the gypsophila paniculata can be improved and the economic value is improved by spraying the plant cell division hormone 6-BA when the cultivated gypsophila paniculata grows to 30-45 days.
According to the purpose of the invention, the invention provides the following technical scheme:
an induction method for promoting the proliferation of the lateral bud of the gypsophila paniculata comprises the following steps:
s1, selecting tissue culture seedlings and soil
Selecting plantlets of gypsophila paniculata which grow well and have no diseases and insect pests, and selecting red soil of mountainous regions of Yunnan plateau as planting soil;
s2, reagent selection
Selecting plant cell division hormone 6-BA (chemical name 6-benzylaminopurine) as division hormone, and its molecular formula is C12H11N5, CAS 1214-39-7;
s3, seedling screening
Selecting tissue culture seedlings which are transplanted into the plug tray and grow for 40 days and have uniform characters;
s4, cultivation
Topping the leaves after the gypsophila paniculata is transplanted to soil for 30 days, and then spraying plant cell division hormone 6-BA on the leaves;
s5, statistics
Respectively spraying plant cell division hormone 6-BA on leaf surfaces of the gypsophila paniculata after topping and 7 days after topping, and respectively counting the germination number of new lateral branches when the gypsophila paniculata grows for 7 days and 14 days;
s6, harvesting
When the growth of the babysbreath branches sprayed with the plant cell division hormone 6-BA reaches 70-100 cm and the flowering rate reaches 75%, the babysbreath branches can be harvested.
Further, in S1, martianus micrantha No. 4 bred by flower institute of rural academy of sciences in Yunnan province is adopted for cultivation and differentiation.
Furthermore, in S4, the concentration of the plant cell division hormone 6-BA sprayed on the leaf surface is 10mg/L, 20mg/L, 30mg/L or 40mg/L.
Furthermore, in S4, the concentration of the plant cell division hormone 6-BA sprayed on the leaf surface is 20mg/L.
The beneficial effects of the invention are:
the invention provides an inducing method for promoting the proliferation of the babysbreath lateral buds, which can induce a large amount of lateral buds by topping branches of the babysbreath and spraying plant cell division hormone 6-BA on leaf surfaces, improve the actual production economic value of the babysbreath, provide technical support for the subsequent nursery stock breeding and lay a foundation for the development of the babysbreath cultivation industry.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1
An induction method for promoting the proliferation of the lateral bud of the gypsophila paniculata comprises the following steps:
s1, selecting tissue culture seedlings and soil
And (3) selecting well-grown gypsophila paniculata seedlings without diseases and insect pests, and culturing and differentiating by adopting gypsophila paniculata No. 4 belonging to the rural institute of Yunnan province.
Selecting red soil of Yunnan plateau mountain land as planting soil;
s2, reagent selection
Selecting plant cell division hormone 6-BA (chemical name 6-benzylaminopurine) as division hormone;
s3, seedling screening
Selecting tissue culture seedlings which are transplanted into the plug tray for 40 days and have uniform growth characters;
s4, cultivation
Topping the panicle after transplanting to soil for 30 days, and then spraying plant cell division hormone 6-BA on leaf surfaces; the concentration of the plant cell division hormone 6-BA sprayed on the leaf surface is 10mg/L, 20mg/L, 30mg/L or 40mg/L.
S5, statistics
Respectively spraying plant cell division hormone 6-BA after topping and 7 days after topping of the gypsophila paniculata, and respectively counting the germination number of newly-grown lateral branches after the gypsophila paniculata grows for 7 days and 14 days;
s6, harvesting
When the growth of the babysbreath branches sprayed with the plant cell division hormone 6-BA reaches 70-100 cm and the flowering rate reaches 75%, the babysbreath branches can be harvested.
Example 2
An induction method for promoting the proliferation of the lateral bud of the gypsophila paniculata comprises the following steps:
s1, selecting tissue culture seedlings and soil
And (3) selecting well-grown gypsophila paniculata seedlings without diseases and insect pests, and culturing and differentiating by adopting gypsophila paniculata No. 4 belonging to the rural institute of Yunnan province.
Selecting red soil of Yunnan plateau mountain land as planting soil;
s2, reagent selection
Selecting plant cell division hormone 6-BA (chemical name 6-benzylaminopurine) as division hormone;
s3, seedling screening
Selecting tissue culture seedlings which are transplanted into the plug tray for 40 days and have uniform growth characters;
s4, cultivation
Topping the leaves after the gypsophila paniculata is transplanted to soil for 30 days, and then spraying plant cell division hormone 6-BA on the leaves; the concentration of the plant cell division hormone 6-BA sprayed on the leaf surface is 20mg/L.
S5, statistics
Respectively spraying plant cell division hormone 6-BA after topping and 7 days after topping of the gypsophila paniculata, and respectively counting the germination number of newly-grown lateral branches after the gypsophila paniculata grows for 7 days and 14 days;
s6, harvesting
When the growth and extension of the gypsophila paniculata branches sprayed with the plant cell division hormone 6-BA reach 70-100 cm and the flowering rate reaches 75%, the plants can be harvested.
Example 3
Control test
Test one: after topping on the same day, respectively treating the cotton seeds with clear water, 10mg/L, 20mg/L, 30mg/L and 40mg/L of plant cytokinin 6-BA, recording the germination rates of 7 th day and 14 th day after treatment, and the statistical results are shown in tables 1-5;
TABLE 1 No-spray medicine (spray clear water) on the same day of topping
TABLE 2 toping and spraying on the same day (6-BA, 10 mg/L)
TABLE 3 toping and spraying on the same day (6-BA, 20 mg/L)
TABLE 4 toping and spraying on the same day (6-BA, 30 mg/L)
TABLE 5 toping and spraying on the same day (6-BA, 40 mg/L)
And (2) testing II: topping on the same day, respectively treating with clear water, 10mg/L, 20mg/L, 30mg/L and 40 mg/L6-BA after 7 days, recording germination rates of 7 days and 14 days after treatment, and the statistical results are shown in tables 6-10;
TABLE 6 No-drug (clear water) after 7 days of toping on the same day
TABLE 7 spraying after topping on the day for 7 days (6-BA, 10 mg/L)
TABLE 8 spraying after 7 days of topping on the day (6-BA, 20 mg/L)
TABLE 9 spraying after 7 days of topping on the day (6-BA, 30 mg/L)
TABLE 10 spraying after 7 days of topping on the day (6-BA, 40 mg/L)
TABLE 11 Effect of different treatments on the Germination percentage of Gypsophila paniculata
The invention relates to an induction method for promoting proliferation of babysbreath lateral buds, which comprises a topping treatment method of branches and a concentration ratio of proliferation hormone. The germination rates of 6-BA (6-benzylamino adenine) adopted by the invention in CK sprayed with clear water on the same topping day when the gypsophila grows to 30 days are respectively 47% and 67% in 7 days and 14 days, the germination rate of gypsophila seedlings sprayed with the topping is highest under the treatment of spraying 20mg/L after the gypsophila seedlings are immediately subjected to induction for 7 days and 14 days, and the germination rates are respectively increased by 112.77% and 76.12% compared with CK; the germination rates of CK sprayed with clear water 7 days after topping on the same day are respectively 58% and 57%; after the Gypsophila paniculata seedlings which are topped for 7 days are sprayed with 6-BA with the concentration of 10mg/L, 20mg/L, 30mg/L and 40mg/L for induction for 7 days and 14 days, the germination rate is also the highest under the treatment of spraying 20mg/L, and the germination rate is improved by 40.35 percent compared with CK after 14 days. According to the method, the germination rate of the lateral branches is highest after the 20 mg/L6-BA spraying treatment on the same day after topping is screened out, the actual production economic benefit of gypsophila paniculata is improved, the technical guarantee is provided for the subsequent seedling breeding, and the foundation is laid for the development of the gypsophila paniculata cultivation industry.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (3)
1. An induction method for promoting the proliferation of a lateral bud of a gypsophila paniculata, which is characterized by comprising the following steps:
s1, selecting tissue culture seedlings and soil
Selecting plantlets of gypsophila paniculata which grow well and have no diseases and insect pests, and selecting red soil of mountainous regions of Yunnan plateau as planting soil;
s2, reagent selection
Selecting plant cell division hormone 6-BA with chemical name of 6-benzylaminopurine as division hormone, and molecular formula of C12H11N5 and CAS 1214-39-7;
s3, seedling screening
Selecting tissue culture seedlings which are transplanted into the plug tray and grow for 40 days and have uniform characters;
s4, cultivation
Topping the leaves after the gypsophila paniculata is transplanted to soil for 30 days, and then spraying plant cell division hormone 6-BA on the leaves; the concentration of the plant cell division hormone 6-BA sprayed on the leaf surface is 10mg/L, 20mg/L, 30mg/L or 40mg/L;
s5, statistics
Respectively spraying plant cell division hormone 6-BA on leaf surfaces of the gypsophila paniculata after topping and 7 days after topping, and respectively counting the germination number of new lateral branches after the gypsophila paniculata grows for 7 days and 14 days;
s6, harvesting
When the growth of the gypsophila paniculata branches sprayed with the plant cell division hormone 6-BA reaches 70-100 cm and the flowering rate reaches 75%, the plants can be harvested.
2. The inducing method for promoting the proliferation of lateral buds of gypsophila paniculata according to claim 1, wherein in S1, gypsophila paniculata No. 4, which is bred by flower institute of rural academy of Yunnan province, is used for cultivation and differentiation.
3. The induction method for promoting the proliferation of the lateral bud of gypsophila paniculata according to claim 1, wherein the concentration of the plant cell division hormone 6-BA sprayed on the leaf surface in S4 is 20mg/L.
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| CN1951174A (en) * | 2005-10-20 | 2007-04-25 | 上海师范大学 | A culture medium composition adaptable for carnation stalk induced bud and application thereof |
| CN106577216B (en) * | 2016-11-04 | 2019-12-13 | 武汉市农业科学院 | Method for promoting germination of lateral buds of succulent plants in Crassulaceae |
| CN107821162B (en) * | 2017-11-07 | 2020-08-21 | 玉溪云星生物科技有限公司 | Large-scale production method of gypsophila paniculata plug seedlings |
| CN107980637A (en) * | 2017-12-21 | 2018-05-04 | 凌工二号（大连）科技有限公司 | Tea-tree tissue culture method |
| CN113080063B (en) * | 2021-04-29 | 2022-06-24 | 河南林业职业学院 | Rapid rooting method for tissue culture of coarse chaff tree |
| CN113475322A (en) * | 2021-05-27 | 2021-10-08 | 中国农业大学 | Cutting seedling method for improving propagation coefficient of daylily by utilizing cytokinin |
| CN113519339A (en) * | 2021-08-12 | 2021-10-22 | 广西壮族自治区中国科学院广西植物研究所 | Method for promoting lateral bud germination of Lepidium clavatum |
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