CN106134836A - A kind of Radix Clematidis cuttage vegetative propagation method - Google Patents

A kind of Radix Clematidis cuttage vegetative propagation method Download PDF

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Publication number
CN106134836A
CN106134836A CN201610506360.8A CN201610506360A CN106134836A CN 106134836 A CN106134836 A CN 106134836A CN 201610506360 A CN201610506360 A CN 201610506360A CN 106134836 A CN106134836 A CN 106134836A
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China
Prior art keywords
cuttage
cutting
seedling
substrate
radix clematidis
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CN201610506360.8A
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Chinese (zh)
Inventor
梁胜仁
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Nanning Guangxi Eco Agriculture Science And Technology Co Ltd
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Nanning Guangxi Eco Agriculture Science And Technology Co Ltd
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Priority to CN201610506360.8A priority Critical patent/CN106134836A/en
Publication of CN106134836A publication Critical patent/CN106134836A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/029Receptacles for seedlings
    • A01G9/0299Handling or transporting of soil blocks or seedlings

Abstract

The invention discloses a kind of Radix Clematidis cuttage vegetative propagation method, comprise the following steps: the collection of cuttage cutting;The preparation in cuttage seedbed, the substrate in seedbed is made up of weight ratio 10:3~5:2~3:3~the peat of 5, mushroom slag, Ramulus Mori powder and Vermiculitum, substrate first with concentration be 0.2% carbendazim medicine liquid spray sterilization reinstall seedling-raising cup;Cutting concentration is 0.2% carbendazim medicinal liquid soaking disinfection, and being then placed in concentration is to soak drawing in diindyl butanoic acid medicinal liquid of 1500mg/L, the cutting that must handle well;By in the cutting cuttage handled well to seedling-raising cup, it is 2~3cm that cutting inserts the degree of depth of substrate, drenches substrate with water after cuttage;Carry out cuttage management and acclimatization and transplants after cuttage, i.e. can get Radix Clematidis seedling.The inventive method cuttage is cultivated 30~40 days rooting rates and is reached more than 90%, and cuttage survival rate can reach 90%.This method one is simple to operate, with low cost, reproduction speed fast, survival rate advantages of higher, can obtain a large amount of Radix Clematidis seedling rapidly, have good promotional value.

Description

A kind of Radix Clematidis cuttage vegetative propagation method
Technical field
The present invention relates to crop breeding technical field, specifically a kind of Radix Clematidis cuttage vegetative propagation method.
Background technology
Radix Clematidis (Clematis chinensi), belongs to Ranunculaceae Clematis medicinal plants, another name radix smilacis sieboldi, Ferrum angle Radix Clematidis, ferrum foot Radix Clematidis, Chinese clematis, creeper are first-class.It is distributed in south of Yunnan, Guizhou, Sichuan, Shaanxi, Guangxi, wide The ground such as east, Hunan, Hubei, Henan, Fujian, Taiwan, Jiangxi, Zhejiang, Jiangsu.The root of Radix Clematidis contains Anemonin, hoary hair Father-in-law's lactone, sterol, saccharide, Saponin lactone, phenols, aminoacid etc., leaf contains lactone, phenols, triterpene, aminoacid, organic acid etc..Prestige Radix Clematidis has the effects such as wind-damp dispelling, the meridian dredging, expectorant saliva, the scattered hypochondriac lump, can treat gout, insensitive impediment, chills and pain of the waist and kness, tinea pedis, malaria, Abdominal mass accumulation, tetanus, the symptom such as tonsillitis, all a fishbone or other bone caught in the throat pharynxs.Owing to Radix Clematidis has the biggest medical value, the market demand Amount is increasing year by year, and price increases therewith.Owing to wild Radix Clematidis cannot meet the demand in market, occur in that in recent years Artificial culture.But at present artificial culture Radix Clematidis exists that seedling quantity is few, reproduction speed slow, and planting process is without normalized management Pattern, the economic benefit causing Radix Clematidis is relatively low, it is difficult to realize industrialized production.
The sapling multiplication mode of Radix Clematidis is mainly based on seminal propagation, and the advantage of seminal propagation is that reproductive number is big, root System is complete, robust growth.But some are susceptible to variation by the flowers of cross-pollination, it is difficult to keep the excellent spy of original kind Levy, and reproduction speed compared with slow, Radix Clematidis seed resource is in short supply, expensive, it is difficult to realize industrialized production.Therefore, find More effectively propagation method be Radix Clematidis in the rain during problem demanding prompt solution.Cottage propagation is asexual propagation, is to take and plants A part for strain nutrition organs, inserts in seedling medium, utilizes its regeneration capacity, be allowed to take root and branch out, become new plant.Cuttage Breeding can keep breediness, and the plant of cultivation is faster than tree seedling growth, can cultivate into seedlings in the short time, but cottage propagation The cutting seedling root system obtained is poor, it is impossible to forming main root, the survival rate of seedling is relatively low, and the life-span is short compared with tree seedling, and resistance is more weak. The research of the cottage propagation being related to Radix Clematidis at present is less, the cuttage breeding method of existing Radix Clematidis exist growing-seedling period length, The problems such as rooting rate is unstable, cutting seedling root system is few, disease resistance is weak, after causing seedling plantation, survival rate is low slow with the speed of growth, It is unfavorable for the large-scale production of Radix Clematidis cottage propagation.
Summary of the invention
The present invention is directed to the problem that Radix Clematidis sapling multiplication exists, it is provided that a kind of simple to operate, with low cost, reproduction speed Hurry up, Radix Clematidis cuttage vegetative propagation method that survival rate is high.
In order to realize object above, the present invention by the following technical solutions:
A kind of Radix Clematidis cuttage vegetative propagation method, comprises the following steps:
S1. cutting collection: the branch of clip 6~8cm is as cutting from Radix Clematidis, branch retains 2~3 leaves, then Blade is cut the area of 2/3, finally cutting lower end is cut into angle shape;
S2. the preparation in cuttage seedbed: the substrate in cuttage seedbed is made up of peat, mushroom slag, Ramulus Mori powder and Vermiculitum, Qi Zhongni The weight ratio of charcoal, mushroom slag, Ramulus Mori powder and Vermiculitum is 10:3~5:2~3:3~5, and after each component matrix mix homogeneously, loading is educated In Seedling cup, the thickness of substrate is 6~8cm, then seedling-raising cup is placed in booth:
S3. cutting processes: cutting concentration is 0.2% carbendazim medicinal liquid soaking disinfection, takes out cutting clear water and rinses, so After put into concentration be 1500mg/L draw in diindyl butanoic acid medicinal liquid soak, the cutting that must handle well;
S4. cuttage: by the cutting cuttage handled well to seedling-raising cup, it is 2~3cm that cutting inserts the degree of depth of substrate, cuttage Drench substrate with water afterwards;
S5. cuttage management: after cuttage above booth cover plastic sheeting, control substrate water content 60~70%, canopy Interior relative air humidity reaches 70~80%, and canopy iuuminting degree is 25~35%;
S6. acclimatization and transplants: cutting cuttage 30~after 40 days rooting rate reach more than 90%, after 5~7 days by plastic sheeting Raise, carry out full exposure seedling exercising, and normally water, seedling exercising 15~20 days, i.e. obtain Radix Clematidis seedling.
Preferably, step S2 substrate mixing after first with concentration be 0.2% carbendazim medicine liquid spray sterilization reinstall seedling-raising cup. Substrate first passes through sterilization can eliminate any pathogenic bacteria that may retain and worm's ovum, it is also possible to during preventing and treating cuttage, disease and insect is to inserting The infringement of fringe.
Preferably, the acquisition time of step S1 cutting is annual 3~April.In south annual 3~the temperature in April 20~ 28 DEG C, air humidity is more suitable for more greatly cultivating seedling.
Preferably, above-described mushroom slag and Ramulus Mori powder are through conventional fermentation, obtain mushroom slag and the Ramulus Mori powder become thoroughly decomposed.
Compared with prior art, beneficial effects of the present invention:
1,30~40 days rooting rates of the inventive method cuttage cultivation reach more than 90%, and the whole cuttage cycle is short, and cuttage becomes Motility rate can reach 90%.
2, the numerous cuttage breeding method of the Radix Clematidis of the present invention i.e. can keep the good characteristic of Radix Clematidis, again can be the biggest Amount breeding seedling.
3, the inventive method also has the advantages such as operation is simple, with low cost, can obtain substantial amounts of at short notice Radix Clematidis seedling, lays a good foundation for Radix Clematidis scale, industrialization plantation, has good social benefit and economy Benefit.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further described, but is not limited to protection scope of the present invention.
Embodiment 1
A kind of Radix Clematidis cuttage vegetative propagation method, comprises the following steps:
S1. cutting collection: the branch of clip 6cm is as cutting from Radix Clematidis for early March, and branch retains 3 leaves, then Blade is cut the area of 2/3, finally cutting lower end is cut into angle shape;
S2. the preparation in cuttage seedbed: the substrate in cuttage seedbed is to be 10:4:2:3 peat, mushroom slag, Ramulus Mori powder by weight ratio Forming with Vermiculitum, after each component matrix mix homogeneously, load in seedling-raising cup, the thickness of substrate is 7cm, then seedling-raising cup is placed in In booth:
S3. cutting processes: cutting concentration is 0.2% carbendazim medicinal liquid soaking disinfection 30min, takes out cutting clear water and rushes Washing, being then placed in concentration is drawing of 1500mg/L to soak 5min, the cutting that must handle well in diindyl butanoic acid medicinal liquid;
S4. cuttage: by the cutting cuttage handled well to seedling-raising cup, it is 2cm that cutting inserts the degree of depth of substrate, uses after cuttage Water is drenched substrate;
S5. cuttage management: after cuttage above booth cover plastic sheeting, control the water content of substrate 65%, empty in canopy Gas relative humidity reaches 70%, and canopy iuuminting degree is 30%;
S6. acclimatization and transplants: after cutting cuttage 40 days, rooting rate reaches 91%, is raised by plastic sheeting after 5 days, carries out complete Illumination seedling exercising, and the humidity of holding substrate of normally watering, seedling exercising 20 days, i.e. obtain Radix Clematidis seedling.
The sum of the present embodiment cutting is 100 strains, survives 86 strains, and cuttage survival rate is 86%.
Embodiment 2
A kind of Radix Clematidis cuttage vegetative propagation method, comprises the following steps:
S1. cutting collection: the branch of clip 8cm is as cutting from Radix Clematidis for late March, and branch retains 2 leaves, then Blade is cut the area of 2/3, finally cutting lower end is cut into angle shape;
S2. the preparation in cuttage seedbed: the substrate in cuttage seedbed is to be 10:3:2:5 peat, mushroom slag, Ramulus Mori powder by weight ratio Forming with Vermiculitum, after each component matrix mix homogeneously, load in seedling-raising cup, the thickness of substrate is 8cm, then seedling-raising cup is placed in In booth:
S3. cutting processes: cutting concentration is 0.2% carbendazim medicinal liquid soaking disinfection 40min, takes out cutting clear water and rushes Washing, being then placed in concentration is drawing of 1500mg/L to soak 8min, the cutting that must handle well in diindyl butanoic acid medicinal liquid;
S4. cuttage: by the cutting cuttage handled well to seedling-raising cup, it is 3cm that cutting inserts the degree of depth of substrate, uses after cuttage Water is drenched substrate;
S5. cuttage management: after cuttage above booth cover plastic sheeting, control the water content of substrate 70%, empty in canopy Gas relative humidity reaches 75%, and canopy iuuminting degree is 35%;
S6. acclimatization and transplants: after cutting cuttage 35 days, rooting rate reaches 93%, is raised by plastic sheeting after 7 days, carries out complete Illumination seedling exercising, and the humidity of holding substrate of normally watering, seedling exercising 15 days, i.e. obtain Radix Clematidis seedling.
The sum of the present embodiment cutting is 100 strains, survives 90 strains, and cuttage survival rate is 90%.
Embodiment 3
A kind of Radix Clematidis cuttage vegetative propagation method, comprises the following steps:
S1. cutting collection: mid-April, the branch of clip 6cm was as cutting from Radix Clematidis, and branch retains 2 leaves, then Blade is cut the area of 2/3, finally cutting lower end is cut into angle shape;
S2. the preparation in cuttage seedbed: the substrate in cuttage seedbed is to be 10:5:3:3 peat, mushroom slag, Ramulus Mori powder by weight ratio Forming with Vermiculitum, after each component matrix mix homogeneously, load in seedling-raising cup, the thickness of substrate is 8cm, then seedling-raising cup is placed in In booth:
S3. cutting processes: cutting concentration is 0.2% carbendazim medicinal liquid soaking disinfection 45min, takes out cutting clear water and rushes Washing, being then placed in concentration is drawing of 1500mg/L to soak 10min, the cutting that must handle well in diindyl butanoic acid medicinal liquid;
S4. cuttage: by the cutting cuttage handled well to seedling-raising cup, it is 2cm that cutting inserts the degree of depth of substrate, uses after cuttage Water is drenched substrate;
S5. cuttage management: after cuttage above booth cover plastic sheeting, control the water content of substrate 60%, empty in canopy Gas relative humidity reaches 80%, and canopy iuuminting degree is 25%;
S6. acclimatization and transplants: after cutting cuttage 30 days, rooting rate reaches 90%, is raised by plastic sheeting after 5 days, carries out complete Illumination seedling exercising, and the humidity of holding substrate of normally watering, seedling exercising 15 days, i.e. obtain Radix Clematidis seedling.
The sum of the present embodiment cutting is 100 strains, survives 88 strains, and cuttage survival rate is 88%.

Claims (2)

1. a Radix Clematidis cuttage vegetative propagation method, it is characterised in that comprise the following steps:
S1. cutting collection: the branch of clip 6~8cm retains 2~3 leaves as cutting, branch from Radix Clematidis, then leaf Sheet cuts the area of 2/3, finally cutting lower end is cut into angle shape;
S2. the preparation in cuttage seedbed: the substrate in cuttage seedbed is made up of peat, mushroom slag, Ramulus Mori powder and Vermiculitum, wherein peat, The weight ratio of mushroom slag, Ramulus Mori powder and Vermiculitum is 10;3~5;2~3;3~5, after each component matrix mix homogeneously, load nursery In Bei, the thickness of substrate is 6~8cm, then seedling-raising cup is placed in booth:
S3. cutting processes: cutting concentration is 0.2% carbendazim medicinal liquid soaking disinfection, takes out cutting clear water and rinses, then puts Entering concentration is to soak drawing in diindyl butanoic acid medicinal liquid of 1500mg/L, the cutting that must handle well;
S4. cuttage: by the cutting cuttage handled well to seedling-raising cup, it is 2~3cm that cutting inserts the degree of depth of substrate, uses after cuttage Water is drenched substrate;
S5. cuttage management: after cuttage above booth cover plastic sheeting, control the water content of substrate 60~70%, empty in canopy Gas relative humidity reaches 70~80%, and canopy iuuminting degree is 25~35%;
S6. acclimatization and transplants: cutting cuttage 30~after 40 days rooting rate reach more than 90%, after 5~7 days, plastic sheeting is lifted Open, carry out full exposure seedling exercising, and normally water, seedling exercising 15~20 days, i.e. obtain Radix Clematidis seedling.
A kind of Radix Clematidis cuttage vegetative propagation method the most according to claim 1, it is characterised in that: step S2 substrate mixes Rear first with concentration be 0.2% carbendazim medicine liquid spray sterilization reinstall seedling-raising cup.
CN201610506360.8A 2016-06-28 2016-06-28 A kind of Radix Clematidis cuttage vegetative propagation method Pending CN106134836A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109089574A (en) * 2018-07-26 2018-12-28 安龙县欣蔓生物科技有限责任公司 A kind of implantation methods of aobvious arteries and veins Inula britannica chinensis

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CN102948323A (en) * 2012-11-20 2013-03-06 浙江虹越花卉有限公司 Method for raising seedlings of clematis by cutting
CN104170616A (en) * 2014-07-18 2014-12-03 宁国千方中药发展有限公司 Artificial cultivation method of radix clematidis
CN104982205A (en) * 2015-07-13 2015-10-21 江苏农林职业技术学院 Method for promoting cutting propagation of clematis--Cardina Rouge

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102132655A (en) * 2010-12-27 2011-07-27 赣州亿圆生物药业有限公司 Planting method of radix clematidis
CN102948323A (en) * 2012-11-20 2013-03-06 浙江虹越花卉有限公司 Method for raising seedlings of clematis by cutting
CN104170616A (en) * 2014-07-18 2014-12-03 宁国千方中药发展有限公司 Artificial cultivation method of radix clematidis
CN104982205A (en) * 2015-07-13 2015-10-21 江苏农林职业技术学院 Method for promoting cutting propagation of clematis--Cardina Rouge

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109089574A (en) * 2018-07-26 2018-12-28 安龙县欣蔓生物科技有限责任公司 A kind of implantation methods of aobvious arteries and veins Inula britannica chinensis

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