CN103609295A - Method for large-scale cultivation of jatropha curcas seedlings - Google Patents

Method for large-scale cultivation of jatropha curcas seedlings Download PDF

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Publication number
CN103609295A
CN103609295A CN201310594702.2A CN201310594702A CN103609295A CN 103609295 A CN103609295 A CN 103609295A CN 201310594702 A CN201310594702 A CN 201310594702A CN 103609295 A CN103609295 A CN 103609295A
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jatropha curcas
branch
scale cultivation
jatropha
seedling
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CN201310594702.2A
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CN103609295B (en
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吴军
陈放
郭亦然
白洁
彭彤
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Sichuan University
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Sichuan University
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Abstract

The invention discloses a method for large-scale cultivation of jatropha curcas seedlings. The method includes the following steps: (1) selecting appropriate jatropha curcas branches, which are more than two years in age, 2-4cm in diameter and free of pest and disease damage and physical damage, with leaves naturally fallen down; (2) sequentially performing pruning, drying, soaking and callusing pretreatment on the selected jatropha curcas branches; (3) preparing nutrient soil, and filling the prepared nutrient soil in a nutrition bowl; (4) when calluses are formed on cuts at ends of the jatropha curcas branches wrapped with films, cutting the jatropha curcas branches into the nutrition bowl; (5) performing cutting seedling management, and transplanting when roots of the cutting jatropha curcas branches are visible on the outer surface of the nutrition soil. The method for large-scale cultivation of the jatropha curcas seedlings is high in survival rate, high-quality seedlings can be effectively provided according to seedling number and season requirements, land resources are fully utilized, and a reliable foundation is laid for large-scale cultivation.

Description

A kind of method of large-scale cultivation Jatropha curcas seedling
Technical field
The present invention relates to a kind of Jatropha curcas seedling-cultivating method, relate in particular to a kind of method of large-scale cultivation Jatropha curcas seedling.
Technical background
Jatropha curcas strong adaptability, drought-resistant barren, environmental friendliness, seed oil content is high, quality better, and being has one of Tree Species as Bio-energy of development potentiality at present most.The development of its industry has obtained extensive concern and the attention of some governments, international organization, research institution and enterprise in the world.The primary raw material that its biofuel is produced is seed oil, and therefore, one of key link of large-scale planting Jatropha curcas Biological Energy Industry development, is the basis of large-scale planting and a large amount of, high quality seedling is provided.
Cuttage and seedling culture is to cut stem from plant parent, a part for root and leaf, under adapt circumstance condition, impel and become the independently seedling-cultivating method of new plant, it is a kind of asexual multiplication seedling, compare seeding and seedling raising breeding, cuttage and seedling culture has the good hereditary capacity of the maternal plant of maintenance, and seedling growth is rapider, fecundity morning etc. feature, be the main seedling-cultivating method of commodity trees, fruit tree and the stock breeding of timber forest wood.Meanwhile, it is all to belong to asexual multiplication seedling that cuttage and seedling culture and tissue rapid propagation are grown seedlings, but cuttage and seedling culture is relatively simpler, growing-seedling period is shorter.For Jatropha curcas, set up corresponding cuttage raise seedling technique, for cultivating good leprosy sapling, lay the foundation.
Summary of the invention
The technical problem to be solved in the present invention provides the method for the large-scale cultivation Jatropha curcas seedling that a kind of cost is low, survival rate is high and production efficiency is high.
The present invention is achieved through the following technical solutions:
A method for large-scale cultivation Jatropha curcas seedling, the method comprises the following steps:
(1) select suitable Jatropha curcas branch, the Jatropha curcas branch of choosing that the age of tree 2 years is above, diameter is 2-4cm, naturally coming off without damage by disease and insect and physically impaired leaf;
(2) to selected Jatropha curcas branch prune successively, be dried, immersion and callus pretreatment; Specific as follows:
1. the pruning of Jatropha curcas branch: shear Jatropha curcas branch and make its length remain on 20-30cm and Jatropha curcas branch two ends are pruned into about 45 ° of inclined-planes; Be conducive to like this branch end that buries and form larger root of hair face, and the other end is conducive to Li Shui, avoids causing rotten at its plane ponding.
2. Jatropha curcas branch is dry: after Jatropha curcas branch two ends are pruned, be placed on about 30min in air, make the liquid natural seasoning of flowing out; Do not need to adopt the solution such as potassium permanganate to carry out disinfection.
3. the immersion of Jatropha curcas branch: Jatropha curcas branch is soaked to position and insert in soak, soak is indolebutyric acid solution, and soak time is 3-5h;
4. the callus pretreatment of Jatropha curcas branch: the Jatropha curcas branch after soaking is placed after 3-5h at 28-30 ℃, and every bundle branch is wrapped and wanted cuttage end with plastic film, and lucifuge is placed 3-7 days at 25-28 ℃, and every 12h opens film 5min midway.
(3) preparation Nutrition Soil, and the Nutrition Soil preparing is packed in nutritive cube; The nutritive cube of the Nutrition Soil filling of preparation has kept breathing freely, loose, water retention property is good, and fertilizer efficiency is gentle, nutrition is applicable to the growth of leprosy sapling.
(4) when the Jatropha curcas branch end incision of film wrapped has callus to form, by Jatropha curcas branch cutting in nutritive cube; Now cuttage survival rate can reach 99%, and while relatively having callus to form, cuttage has not improved survival rate greatly.
(5) carry out cuttage seeding period management, when the root of Jatropha curcas cuttage branch is transplanted when the Nutrition Soil outer surface being shaped is visible.Under the environment of suitable planting, now transplanting survival rate can be up to 99%, and growth is fast, conventionally in transplanting, can yield positive results 2-4 time in latter 12 months.During transplanting, together with nutritive cube, transport, remove nutritive cube and be with soil plantation.
As preferably, the concentration of described indolebutyric acid solution is 5.0-10.0PPM.
As preferably, described Nutrition Soil is comprised of coconut palm chaff, sand grains and the excrement of animals that becomes thoroughly decomposed, and coconut palm chaff: sand grains: excrement of animals=1:1:1 becomes thoroughly decomposed.
As preferably, the particle diameter of described sand grains is 0.1-1mm.
As preferably, the diameter of described nutritive cube be 10cm, high be 15cm.
Particularly, the Jatropha curcas branch cutting degree of depth of described step (4) is 7-10cm, has 3-5 axillalry bud to be exposed in air on described Jatropha curcas branch.
Particularly, during cuttage seeding period management, nutritive cube is placed on to Li Shui, sun-drenched place, environmental temperature is 25-35 ℃.Start to block 10 days with sunshade net, remove afterwards sunshade net.
Particularly, by 3-5 days after nutritive cube, adopt water once morning Jatropha curcas branch cutting, before transplanting, adopt afterwards the mode of sooner or later respectively watering once.
The invention has the beneficial effects as follows:
The method of large-scale cultivation Jatropha curcas seedling provided by the invention, survival rate is high, not only can according to seedling quantity and season needs, high quality seedling is provided effectively, also for making full use of land resources, realize large-scale planting and establish reliable basis.Nutrition Soil formula of the present invention can effectively form a large amount of roots simultaneously, has both been conducive to the growth of cuttage seeding wood, also helps the growth of nursery stock after transplanting.
Embodiment
The method of large-scale cultivation Jatropha curcas seedling of the present invention; the method comprises the following steps: (1) is according to maternal plant hereditary capacity or plantation demand; the Jatropha curcas branch of cuttage and seedling culture is carried out in collection, the Jatropha curcas branch that gathers that the maternal plant age of tree 2 years is above, branch diameter is 2-4cm, naturally comes off without damage by disease and insect and physical damnification, leaf.
(2) the Jatropha curcas branch gathering is carried out to pretreatment: Jatropha curcas branch is pruned successively, immersion and callusization are processed.To the trimming method of branch, be: shear branch, make its length remain on 20-30cm; Cuttage branch two ends ends is pruned into about 45 ° of inclined-planes, be conducive to like this branch end that buries and form larger root of hair face, and the other end is conducive to Li Shui, avoids at its plane ponding, causes rotten; To the immersion process of branch, be: after branch two ends are pruned, be placed on about 30min in air, make the liquid natural seasoning of outflow, do not need to adopt the solution such as potassium permanganate to carry out disinfection; It is will insert in soak that branch soaks position, and soaking solution used is indolebutyric acid solution, and indolebutyric acid solution concentration used is 5.0-10.0PPM, and soak time is 3-5h; To soaking the callus method of rear branch, be: by 50 strain a bundles, process after 3-5h for 28-30 ℃, every bundle branch is wrapped and wanted cuttage end with plastic film that lucifuge is placed 3-5 days at 26-28 ℃.
(3) preparation Nutrition Soil, and carry out nutritive cube filling.The raw material that in the present invention, nutritive soil preparation is used is coconut palm chaff, sand grains and the excrement of animals that becomes thoroughly decomposed, and the particle diameter of sand grains is 0.1-1mm, the usage ratio of raw material is coconut palm chaff: sand grains: excrement of animals=1:1:1 becomes thoroughly decomposed.After preparing Nutrition Soil, Nutrition Soil is inserted to the narrow meshed nutritive cube in bottom, the diameter of nutritive cube is 10cm, and height is 15cm.The nutritive cube of the Nutrition Soil filling of preparing in the present invention has kept breathing freely, loose, water retention property is good, and fertilizer efficiency is gentle, nutrition is applicable to the growth of leprosy sapling.
(4) using branch incision callus and whether form as basis for estimation, in good time by Jatropha curcas branch cutting in nutritive cube.Callus to be had forms and can be used for cuttage at cutting section, and now cuttage survival rate can reach 99%, and while relatively having callus to form, cuttage has not improved survival rate greatly.During cuttage, branch inserts Nutrition Soil degree of depth 7-10cm, and keeps having 3-6 axillalry bud to be exposed in air on branch.
(5) carry out cuttage seeding period management, the good nutritive cube of cuttage is placed on to Li Shui, sun-drenched place, environmental temperature is 25-35 ℃, starts to block 10 days with sunshade net, removes afterwards sunshade net; Different growing stages, the mode of watering difference, after cuttage completes, adopts water once morning in front 3-5 days, sooner or later respectively waters once afterwards before transplanting.According to the growing state judgement Different Transplanting Periods of cuttage branch root.Now Nutrition Soil is substantially shaped in nutritive cube, and root, when the Nutrition Soil outer surface being shaped is visible, can be transplanted.Under the environment of suitable planting, now transplanting survival rate can be up to 99%, and growth is fast, conventionally in transplanting, can yield positive results 2-4 time in latter 12 months.During transplanting, together with nutritive cube, transport, remove nutritive cube and be with soil plantation.
The present invention is directed to the hereditary capacity that retains Jatropha curcas maternal plant, set up simply, cultivated efficiently a large amount of high-quality Jatropha curcas nursery stocks, for being engaged in the related organizations such as peasant household, company and scientific research of Jatropha curcas plantation, provide effective seedling-cultivating method.

Claims (8)

1. a method for large-scale cultivation Jatropha curcas seedling, is characterized in that: the method comprises the following steps:
(1) select suitable Jatropha curcas branch, the Jatropha curcas branch of choosing that the age of tree 2 years is above, diameter is 2-4cm, naturally coming off without damage by disease and insect and physically impaired leaf;
(2) to selected Jatropha curcas branch prune successively, be dried, immersion and callus pretreatment; Specific as follows:
1. the pruning of Jatropha curcas branch: shear Jatropha curcas branch and make its length remain on 20-30cm and Jatropha curcas branch two ends are pruned into about 45 ° of inclined-planes;
2. Jatropha curcas branch is dry: after Jatropha curcas branch two ends are pruned, be placed on about 30min in air, make the liquid natural seasoning of flowing out;
3. the immersion of Jatropha curcas branch: Jatropha curcas branch is soaked to position and insert in soak, soak is indolebutyric acid solution, and soak time is 3-5h;
4. the callus pretreatment of Jatropha curcas branch: the Jatropha curcas branch after soaking is placed after 3-5h at 28-30 ℃, and lucifuge is placed 3-7 days at 25-28 ℃, and every 12h opens film 5min midway.
(3) preparation Nutrition Soil, and the Nutrition Soil preparing is packed in nutritive cube;
(4) when the Jatropha curcas branch end incision of film wrapped has callus to form, by Jatropha curcas branch cutting in nutritive cube;
(5) carry out cuttage seeding period management, when the root of Jatropha curcas cuttage branch is transplanted when the Nutrition Soil outer surface being shaped is visible.
2. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 1, is characterized in that: the concentration of described indolebutyric acid solution is 5.0-10.0PPM.
3. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 1, is characterized in that: described Nutrition Soil is comprised of coconut palm chaff, sand grains and the excrement of animals that becomes thoroughly decomposed, and coconut palm chaff: sand grains: excrement of animals=1:1:1 becomes thoroughly decomposed.
4. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 3, is characterized in that: the particle diameter of described sand grains is 0.1-1mm.
5. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 1, is characterized in that: the diameter of described nutritive cube is 10cm, the high 15cm of being.
6. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 1, is characterized in that: the degree of depth that the Jatropha curcas branch of described step (4) inserts Nutrition Soil is 7-10cm, has 3-5 axillalry bud to be exposed in air on described Jatropha curcas branch.
7. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 1, is characterized in that: during cuttage seeding period management, nutritive cube is placed on to Li Shui, sun-drenched place, environmental temperature is 25-35 ℃.
8. the method for a kind of large-scale cultivation Jatropha curcas seedling according to claim 1, is characterized in that: Jatropha curcas branch cutting, within 3-5 days, adopting water once morning after nutritive cube, is adopted to the mode of sooner or later respectively watering once afterwards before transplanting.
CN201310594702.2A 2013-11-21 2013-11-21 A kind of method for large-scale cultivation of jatropha curcas seedlings Expired - Fee Related CN103609295B (en)

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Cited By (11)

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CN105393883A (en) * 2015-12-02 2016-03-16 黄钱英 Large scale Lithocarpushancei(Benth.)Rehd seedling cultivation method
CN105393779A (en) * 2015-12-02 2016-03-16 黄钱英 Large scale Lithocarpuscalophyllus seedling cultivation method
CN105393778A (en) * 2015-12-02 2016-03-16 黄钱英 Large scale Lithocarpusamygdalifolius(Skan)Hayata seedling cultivation method
CN105409548A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus elmerrillii seedlings
CN105409687A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Quercus variabilis Bl seedlings
CN105409550A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus craibianus Barn. seedlings
CN105409688A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus carolinae (Skan) Rehd seedlings
CN105409549A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus oleaefolius A. Camus seedings
CN105432405A (en) * 2015-12-02 2016-03-30 黄钱英 Method for cultivating lithocarpus areca seedlings in large scale
CN107094606A (en) * 2017-05-10 2017-08-29 麦丽 A kind of cuttage and seedling culture method of Jatropha curcas
CN112273072A (en) * 2020-10-29 2021-01-29 神农架国家公园科学研究院 Cutting seedling method of Yichang machilus

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105393883A (en) * 2015-12-02 2016-03-16 黄钱英 Large scale Lithocarpushancei(Benth.)Rehd seedling cultivation method
CN105393779A (en) * 2015-12-02 2016-03-16 黄钱英 Large scale Lithocarpuscalophyllus seedling cultivation method
CN105393778A (en) * 2015-12-02 2016-03-16 黄钱英 Large scale Lithocarpusamygdalifolius(Skan)Hayata seedling cultivation method
CN105409548A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus elmerrillii seedlings
CN105409687A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Quercus variabilis Bl seedlings
CN105409550A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus craibianus Barn. seedlings
CN105409688A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus carolinae (Skan) Rehd seedlings
CN105409549A (en) * 2015-12-02 2016-03-23 黄钱英 Method for culturing large-scale Lithocarpus oleaefolius A. Camus seedings
CN105432405A (en) * 2015-12-02 2016-03-30 黄钱英 Method for cultivating lithocarpus areca seedlings in large scale
CN107094606A (en) * 2017-05-10 2017-08-29 麦丽 A kind of cuttage and seedling culture method of Jatropha curcas
CN112273072A (en) * 2020-10-29 2021-01-29 神农架国家公园科学研究院 Cutting seedling method of Yichang machilus
CN112273072B (en) * 2020-10-29 2022-03-15 神农架国家公园科学研究院 Cutting seedling method of Yichang machilus

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