CN109121783A - The engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part - Google Patents

The engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part Download PDF

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Publication number
CN109121783A
CN109121783A CN201810984553.3A CN201810984553A CN109121783A CN 109121783 A CN109121783 A CN 109121783A CN 201810984553 A CN201810984553 A CN 201810984553A CN 109121783 A CN109121783 A CN 109121783A
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China
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scion
oil
radicle
stock
tea
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CN201810984553.3A
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Inventor
徐德兵
陈福
张林涛
闫争亮
贾代顺
郭晓春
廖永坚
刘倬志
宋顺超
袁其琼
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Oil Tea Institute Yunnan Academy Of Forestry
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Oil Tea Institute Yunnan Academy Of Forestry
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Priority to CN201810984553.3A priority Critical patent/CN109121783A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Cultivation Of Plants (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention discloses a kind of engrafting methods that twig will not be sprouted using oil-tea sprout radicle graft stock part.To Sand culture is carried out after Seed of Camellia oleifera and the disinfection of thin river sand, until taking out sprout, 1 minute use to be grafted of sterilizing after sprout cleaning from river sand after second year oil tea in 5~June grafting fringe bar semi-lignified;Bud is full on rooting rate, oil tea semi-lignified branch of no disease and pests harm is as scion, and scion has one leaf of a bud, cuts off the 1/3 or 1/2 of scion leaf, and the inclined-plane of two a length of 1.30~1.60cm is cut in the bottom end of scion, two inclined-planes are crossed as 45° angle, and bottom end intersects at medulla, and mouth is cut in formation;Select oil-tea sprout radicle part as graft stock;Then mouthful length and scion base diameter are cut according to scion, cuts out graft union in radicle stock upper center, and stock is inserted into the scion cut, set is pricked rapidly, completes grafting.During solving conventional graft seedling growth, need constantly except the problem of sprouting.

Description

The engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part
Technical field
The invention belongs to technical field of forestry, utilize oil-tea sprout radicle graft stock part will not more particularly to a kind of Sprout the engrafting method of twig.
Background technique
Oil tea Camellia oleifera Abel is Theaceae Camellia evergreen shrubs or dungarunga, is the peculiar wood in China This oil tree.Unsaturated fatty acid and content of polyunsaturated fatty acid are up to 90% or more in tea oil, while being rich in vitamin E, the various trace elements such as vitamin D, carrotene, food, drug and in terms of obtained extensive exploitation benefit With.China is that the distribution, variation and hereditary center, the oil tea resource in 80% or more the whole world of world's oil tea resource are distributed mainly on The Yangtze river basin and its on the south 14 provinces (area, city).Woody oleiferous plants industry is greatly developed in country, it is ensured that the strategy of grain and oil safety Under deployment, the development of national camellia oleiferaindustry is very rapid, and whole nation camellia oleifera lam area is up to 4,000,000 hm at present2, according to " national oil tea produces Industry development plan ", national camellia oleifera lam area of base is up to 467.87 ten thousand hm to 20202.Nursery stock production is industry development Important key link, in production, oil tea cultivating seedlings are essentially all to be cultivated using conventional Nurse seed grafting method at present, Wherein graft stock all selects the sprout plumule formed after Seed of Camellia oleifera sand storage vernalization, and also seeing has the report for making stock using plumular axis Road.No matter with plumule make stock grafting seedling, or use plumular axis as stock grafting seedling, after the transfer, grafts plumule or embryo Axis (stock) part can all gradually grow up with the growth of seedling as the trunk of nursery stock;Meanwhile nursery stock trunk (stock) position Also continuous rudiment and pumping branch can be sent out with the growth of nursery stock trunk, and constantly consumes the nutriment of nursery stock, and then influence Nursery stock grows up strong and sturdy.Therefore, during cultivating seedlings, nursery stock trunk (stock) position is one essential except work is sprouted And highly important work, this work not only cumbersome time-consuming, operating difficulties, but also need to consume a large amount of human and material resources. It is generally transplanted to before nursery is out of the garden to seedling from grafting seedling, at least needs to carry out 2~3 times except sprouting work.In particular, removing every time It sprouts and requires to open the progress of nursery plastic canopy, if technical treatment is improper, also will cause a large amount of atrophys of nursery stock or death, in addition often It is secondary all to affect to the growth of nursery stock when opening nursery plastic canopy, it is artificial can also be to grafting seedling except when sprouting Cause certain artificial damage.It is asked except the bottleneck for sprouting work always puzzlement camellia seedling at oil tea stock (nursery stock trunk) position Topic, not yet makes a breakthrough so far.
Summary of the invention
Transferring for twig will not be sprouted using oil-tea sprout radicle graft stock part the purpose of the present invention is to provide a kind of Method is connect, to solve in routine plumule or plumular axis stock grafting seedling raising process, after grafting seedling transplanting, plumule and plumular axis part It can be grown with seedling and constantly take out hair branch and rudiment, to influence seedling growth, need constantly to carry out the bottleneck except sprouting.
The technical scheme adopted by the invention is that transferring for twig will not be sprouted using oil-tea sprout radicle graft stock part Connect method, which comprises the following steps:
Step 1: oil-tea sprout is cultivated: annual October acquires high-quality oil tea ripening fruits, and shelling, which is dried in the shade 3 days, forms oil tea Seed;Sand culture is carried out after sterilizing to Seed of Camellia oleifera and thin river sand;Seed of Camellia oleifera is located among thin river sand when sand storage, above and below The thin river sand of layer is with a thickness of 15cm;It is 60% that river sand humidity is kept during sand storage;Sand storage to 5~June of second year, oil tea is grafted After fringe bar semi-lignified, sprout is taken out from river sand, sterilizing 1 minute after sprout cleaning, use to be grafted;
Step 2: cutting scion: selecting in sprout that bud is full, semi-lignified oil tea branch of no disease and pests harm is as scion, connect Fringe has one leaf of a bud, cuts off the 1/3 or 1/2 of scion leaf, and cut two a length of 1.30~1.60cm's in the bottom end of scion Inclined-plane, two inclined-planes are crossed as 45° angle, and bottom end intersects at medulla, and mouth is cut in formation;
Step 3: radicle stock is handled: more than removal oil-tea sprout radicle plumule, cotyledon and plumular axis retain radicle portion It is allocated as graft stock;Then mouthful length and scion base diameter are cut according to scion, cuts out graft union in radicle stock upper center;
Step 4: grafting: after stock is inserted into the scion cut, set is pricked rapidly, completes grafting;
Step 5: grafting seedling is moved bag, neatly it is placed in nursery lot and cultivates oil tea seedling.
Further, fungicide used in sterilizing is that carbendazim 50% is wettable after oil-tea sprout cleaning in the step 1 Property 700-800 times of liquid of pulvis or Mancozeb.
Further, scion base diameter is 0.3~0.5cm in the step 3.
Further, the graft union length in the step 3 on radicle stock is 1.30~1.60cm.
Further, the step 4 is pricked using aluminium skin or thin-film covering;It is pricked using aluminium leather sheath, 2 foldings need to be carried out to aluminium skin It is folded;It is pricked using thin-film covering, envelope need to be carried out to film and is tied up.
The invention has the advantages that the grafting side of twig will not be sprouted using oil-tea sprout radicle graft stock part Method, using oil-tea sprout radicle during cultivating seedlings will not rudiment, take out hair branch characteristic, change conventional plumule anvil The wooden grafting method, solve it is previous no matter with plumule or plumular axis make stock grafting seedling, after the transfer, plumule and plumular axis (stock) Part can all be grown with seedling constantly takes out hair branch and rudiment, and then constantly consumes seedling-nourishing substance, influence seedling at It is long, thus must carry out cumbersome time-consuming except the problem of sprouting work.The seedling grafted using radicle as graft stock, is had During effect solves conventional graft seedling growth, need constantly except the cultivation program sprouted, at the same decrease it is right during except sprouting Influence caused by seedling growth and artificial damage.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with It obtains other drawings based on these drawings.
Fig. 1 is the complete oil-tea sprout schematic diagram of Sand culture;
Fig. 2 is each position schematic diagram of oil-tea sprout;
Fig. 3 is scion schematic diagram;
Fig. 4 is plumule stock grafting schematic diagram;
Fig. 5 is plumular axis stock grafting schematic diagram;
Fig. 6 is radicle stock grafting schematic diagram.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other Embodiment shall fall within the protection scope of the present invention.
The engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part, comprising the following steps:
Step 1: oil-tea sprout is cultivated: annual October acquires high-quality oil tea ripening fruits, after shelling is dried in the shade 3 days, to oil tea Seed and thin river sand carry out disinfection processing, then carry out Sand culture to Seed of Camellia oleifera with thin river sand;Seed of Camellia oleifera position when sand storage Among thin river sand, seed upper and lower level is respectively the thin river sand of thick 15cm;It is 60% that river sand humidity is kept during sand storage;Such as Fig. 1 Shown, sand storage to 5~June of second year after oil tea grafts fringe bar semi-lignified, sprout is taken out from river sand, is put after sprout cleaning Enter carbendazim 700-800 times of liquor of 50% wettable powder;Sterilizing 1 minute, oil-tea sprout was spare after taking out disinfection.
Seed of Camellia oleifera is located among thin river sand, and in sprout incubation, this 3 parts of plumule, plumular axis, cotyledon are to river sand Upper grown, radicle part is to some growth under river sand.If river sand is too thick, the mould bad rate of seed can be increased;River sand is too thin, river Husky Water-saving effect is deteriorated, and influences the germination percentage of sand storage seed, and sand storage seed in October current year, until 5~June of second year, sprout Length be more than river sand thickness, influence sprout growth.Usual sand storage is all that the exposed world out of office carries out, therefore, in sand storage process In to observe river sand humidity in real time, with hand pinch it is agglomerating, loose one's grip i.e. dissipate be preferred.If river sand is too dry, it can suitably increase irrigation amount, one As when temperature is more than 12 DEG C, seed starts germinating growth.Before grafting, it is unearthed in case of sprout growth is too fast, river sand can be covered and covered Lid;Through testing, this paper Seed of Camellia oleifera is located among thin river sand, the thin river sand of seed upper and lower level with a thickness of 15cm, effect is best.
Step 2: cutting scion: selecting the semi-lignified oil tea branch of full bud eye, no disease and pests harm, 0.3~0.5cm of base diameter As scion, scion has one leaf of a bud, and blade cuts off 1/3 or 1/2, then, respectively cut in the bottom end of scion and be about 1.3~ Two inclined-planes of 1.6cm, two inclined-planes are crossed as 45° angle, and intersect at medulla, and mouth is cut in formation, as shown in Figure 3.
Scion base diameter is selected according to sprout stock rugosity, and scion base diameter is excessively thick, it is corresponding with grafting sprout stock not on; If scion base diameter is too thin, poor quality;Scion base diameter is excessively thick or the meticulous graft survival rate that will lead to is lower, herein through trying It tests, selection scion base diameter is 0.3~0.5cm.Stock rugosity is too small, and grafting fringe bar can not be wrapped in centre, and grafting fringe bar has Partial denudation is outside stock, and survival rate is very poor after grafting.Scion has one leaf of a bud, and blade cuts off 1/3 or 1/2, effectively reduces Moisture evaporation, reduces the loss of scion moisture itself, increases the activity of scion, improves graft survival rate.The bottom end of scion is each Two inclined-planes for being about 1.30~1.60cm are cut, two inclined-planes are crossed as 45° angle, and intersect at medulla, so that scion is easy when grafting It is inserted into the graft union of stock.
Step 3: radicle stock is handled: as shown in Fig. 2, oil-tea sprout is divided into plumule, cotyledon, plumular axis and embryo from top to bottom 4 part of root;With plumule, cotyledon and plumular axis more than grafting knife or blade removal radicle, only retain radicle part as grafting anvil Wood;Then according to fringe bar grafting cut a mouthful length, with grafting knife or blade cut out stock upper center cut out length be 1.30~ 1.60cm graft union.
The purpose of grafting is that the forming layer of scion and the forming layer of stock are docking together, and forming layer is scion and stock Nutriment connect unclog the pipe, graft union it is too short or it is too long can all influence cambial docking, if the shape of scion and stock Stratification cannot be docking together, and seedling will not survive after grafting, and the graft union length of this paper radicle stock is 1.30~1.60cm Most preferably.
Step 4: grafting: after stock is inserted into the scion cut, being pricked with aluminium skin or thin-film covering rapidly, such as use aluminium skin, be Keep aluminium suitcase tight, 2 foldings need to be carried out to aluminium skin;Film is such as used, envelope need to be carried out to grafting and is tied up, as shown in Figure 6.
Step 5: grafting seedling is moved bag, neatly it is placed in nursery lot and cultivates seedling.
In carbendazim 700-800 times of liquid of 50% wettable powder in step 1,50% wettable powder of carbendazim and water Mass ratio be 1:700~800, also can be used other disinfection drugs such as Mancozeb substitute.
Embodiment 1
Same camellia oleifera cultivar fringe bar is acquired in Yunnan Province Guangnan County and is utilized respectively embryo as shown in figures 4-6 in May, 2014 Bud, plumular axis and radicle are graft stock, each 200 plants of nursery stock of grafting, and grafting is moved back to cultivating nursery stock in nursery lot.
Reinforce nursery stock water and fertilizer management in May, 2014~2016 year June, carry out except sprout, weeding work, investigate transferring for nursery stock Be connected into motility rate, graft stock branch out germination rate, except number is sprouted, in June, 2016, investigation cultivates nursery stock using different graft stocks Height of seedling, ground diameter, survival rate, pilot survey result such as the following table 1.
The different graft stocks of table 1 graft 2 years raw seedling survey tables
Since grafting seedling is that fringe bar and stock are bundled together with aluminium skin or film, seedling is grafted in addition inherently very It is small, during except sprouting, if accidentally, on the one hand will cause the loosening of graft union, on the other hand also having will cause fringe bar just The young tender sprout of sprouting falls off or is broken, and influences graft survival rate and seedling quality.By test statistics result table 1 it is found that utilizing Oil-tea sprout radicle makees stock, during seedling growth, stock individual developmental be nursery stock trunk after will not rudiment, take out hair branch Item, and oil tea nursery stock height of seedling after grafting and average ground diameter are above plumule stock and plumular axis stock, the oil tea nursery stock of cultivation is not It is only tall and big and sturdy, efficiently solve it is previous no matter with plumule or plumular axis make stock grafting seedling, after grafting seedling transplanting, Plumule and plumular axis (stock) part can all grow with seedling and constantly take out hair branch and rudiment, so that seedling growth is influenced, and It needs constantly to carry out to decrease manpower and material resources except the bottleneck sprouted, seedling growth is made during removing and sprouting in particular, reducing At influence and artificial damage, achieve good Relationship between Graft Effect.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.It is all Any modification, equivalent replacement, improvement and so within the spirit and principles in the present invention, are all contained in protection scope of the present invention It is interior.

Claims (5)

1. the engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part, which is characterized in that including following Step:
Step 1: oil-tea sprout is cultivated: annual October acquires high-quality oil tea ripening fruits, and shelling, which is dried in the shade 3 days, forms Seed of Camellia oleifera; Sand culture is carried out after sterilizing to Seed of Camellia oleifera and thin river sand;Seed of Camellia oleifera is located among thin river sand when sand storage, upper and lower level Thin river sand is with a thickness of 15cm;It is 60% that river sand humidity is kept during sand storage;Sand storage to 5~June of second year, oil tea grafts fringe bar After semi-lignified, sprout is taken out from river sand, sterilizing 1 minute after sprout cleaning, use to be grafted;
Step 2: cutting scion: selecting that bud is full, semi-lignified oil tea branch of no disease and pests harm is as scion, scion has one One leaf of bud cuts off the 1/3 or 1/2 of scion leaf, and cuts the inclined-plane of two a length of 1.30~1.60cm in the bottom end of scion, and two tiltedly Face is crossed as 45° angle, and bottom end intersects at medulla, and mouth is cut in formation;
Step 3: radicle stock is handled: more than removal oil-tea sprout radicle plumule, cotyledon and plumular axis retain radicle part and make For graft stock;Then mouthful length and scion base diameter are cut according to scion, cuts out graft union in radicle stock upper center;
Step 4: grafting: after stock is inserted into the scion cut, set is pricked rapidly, completes grafting;
Step 5: grafting seedling is moved bag, neatly it is placed in nursery lot and cultivates oil tea seedling.
2. the engrafting method according to claim 1 that twig will not be sprouted using oil-tea sprout radicle graft stock part, It is characterized in that, fungicide used in sterilizing is 50% wettable powder of carbendazim after oil-tea sprout cleaning in the step 1 700-800 times of liquid or Mancozeb.
3. the engrafting method according to claim 1 that twig will not be sprouted using oil-tea sprout radicle graft stock part, It is characterized in that, scion base diameter is 0.3~0.5cm in the step 3.
4. the engrafting method according to claim 1 that twig will not be sprouted using oil-tea sprout radicle graft stock part, It is characterized in that, the graft union length in the step 3 on radicle stock is 1.30~1.60cm.
5. the engrafting method according to claim 1 that twig will not be sprouted using oil-tea sprout radicle graft stock part, It is characterized in that, the step 4 is pricked using aluminium skin or thin-film covering;It is pricked using aluminium leather sheath, 2 foldings need to be carried out to aluminium skin;It uses Thin-film covering is pricked, and envelope need to be carried out to film and is tied up.
CN201810984553.3A 2018-08-28 2018-08-28 The engrafting method of twig will not be sprouted using oil-tea sprout radicle graft stock part Pending CN109121783A (en)

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CN110547108A (en) * 2019-09-29 2019-12-10 贵州黔西南喀斯特区域发展研究院 Method for reducing germination amount of camellia oleifera grafting rootstock
CN111657032A (en) * 2020-05-06 2020-09-15 六安市裕安区伊甸园油茶种植专业合作社 Camellia oleifera root grafting propagation method
CN115226511A (en) * 2022-08-11 2022-10-25 湖南新创四季生态农业发展有限公司 Stock root grafting method for improved oil tea
CN116584258A (en) * 2023-06-09 2023-08-15 浙江省柑橘研究所 Citrus hybrid seed embryo grafting method
CN117356272A (en) * 2023-11-14 2024-01-09 福建省平和天马国有林场 Grafting seedling raising method for agilawood easy to form and high in survival rate

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Publication number Priority date Publication date Assignee Title
CN110547108A (en) * 2019-09-29 2019-12-10 贵州黔西南喀斯特区域发展研究院 Method for reducing germination amount of camellia oleifera grafting rootstock
CN110547108B (en) * 2019-09-29 2021-12-03 贵州黔西南喀斯特区域发展研究院 Method for reducing germination amount of camellia oleifera grafting rootstock
CN111657032A (en) * 2020-05-06 2020-09-15 六安市裕安区伊甸园油茶种植专业合作社 Camellia oleifera root grafting propagation method
CN115226511A (en) * 2022-08-11 2022-10-25 湖南新创四季生态农业发展有限公司 Stock root grafting method for improved oil tea
CN116584258A (en) * 2023-06-09 2023-08-15 浙江省柑橘研究所 Citrus hybrid seed embryo grafting method
CN116584258B (en) * 2023-06-09 2024-04-16 浙江省柑橘研究所 A citrus hybrid seed embryo grafting method
CN117356272A (en) * 2023-11-14 2024-01-09 福建省平和天马国有林场 Grafting seedling raising method for agilawood easy to form and high in survival rate

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Application publication date: 20190104