CN102334468A - High-efficiency floating seedling Aphidius gifuensis propagation method - Google Patents
High-efficiency floating seedling Aphidius gifuensis propagation method Download PDFInfo
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Abstract
A high-efficiency floating seedling Aphidius gifuensis propagation method includes the following steps: cultivating tobacco seedlings as host plants; introducing and propagating tobacco aphides: when three to four main leaves grow on each of the tobacco seedlings in the previous step, parent aphides are introduced into the tobacco seedlings and freely diffused and propagated, and the tobacco seedlings are covered by insect-proof nets; introducing and propagating Aphidius gifuensis: needed adult Aphidius gifuensis is introduced into the insect-proof nets, and thereby cannot escape, the adult Aphidius gifuensis is promoted to lay parasitic eggs in the bodies of the tobacco aphides, so that the tobacco aphides become stiff aphides, Aphidius gifuensis then emerges, seedling growing is carried out in a seedling-growing pool of an empty seedling-growing greenhouse or a small field greenhouse after tobacco transplantation, 162-mesh or 200-mesh floating trays are used for alternate-row sowing and floating seedling growing, the needed adult Aphidius gifuensis is introduced into the covering nets according to the ratio of the Aphidius gifuensis to the aphides which is 1:100, the temperature in the covering nets is kept at 20 DEG C to 30 DEG C, and after 10 to 15 days, a large quantity of stiff aphides are formed and Aphidius gifuensis emerges. The method utilizes conventional seeding-growing facilities to propagate the Aphidius gifuensis, thus greatly saving the cost, the floating seedlings are low in density, so that the host tobacco aphides can be efficiently propagated, the compulsory Aphidius gifuensis introduction method with the insect-proof nets ensures that the Aphidius gifuensis can be efficiently parasitized in the tobacco aphides, the Aphidius gifuensis propagation period is shortened to less than two months, the quantity of stiff aphides or parasitized aphides per square meter is up to more than one hundred thousand, and the effect is remarkable.
Description
Technical field
The invention belongs to one and grow tobacco under the technology, particularly low density condition of plant protection, Floating seedling is efficiently bred the aphidius gifuensis method.
Background technology
In the flue-cured tobacco cultivation process, usually can run into many insect pests, wherein the most common, the most multiple insect will have been counted aphid.Cigarette aphid thorn smoking blade of grass sheet, stem stalk and floral axis juice like intensive on leaf back or lobus cardiacus, and blade is injured and faded spot occurred, and serious jaundice is crispaturaed, distortion or withered.Tender stem, bennet are injured and are deformity, and it is undesired or withered that capsule is grown.Can also propagate the cucumber mosaic viral disease in addition, tobacco is caused bigger production loss.
The host plant of cigarette aphid still has 300 various plants such as brassicaceous vegetable, capsicum, potato, eggplant, melon, soybean, peach, Lee, apricot, plum except that tobacco.This worm is respectively planted the cigarette district in China all to be had and takes place and cause harm, and serious.The cigarette aphid is got food cigarette strain juice with sucking mouth parts, makes the cigarette strain poor growth of being injured, and quality descends, and alatae can also be propagated several diseases viral diseases such as cucumber mosaic, and tobacco is caused even more serious loss.
Control to the cigarette aphid is main with agricultural chemicals at present; Ward off 2000～3000 times of liquid of aphid mist wetting powder as begin to spray 2000 times of liquid of homemade 50% Aphox wetting powder or Britain product 50% at aphid spot film stage of development; This medicine selectivity is strong; Only effective to aphid, harmless to beneficial insects such as natural enemy insect and silkworm, honeybees, help the ecological balance in field.Also can select for use 3000 times of liquid of 1.8% avermectin missible oil or 16% along 1500 times of liquid of rich No. 3 missible oil, 4000 times of liquid of 5.7% cyfluthrin missible oil, 2500 times of liquid of 10% imidacloprid wettable powder, 1500 times of liquid of the special suspending agent of 5% sharp strength, 2000 times of liquid of 4.5% efficient cis-, trans-cypermethrin missible oil etc.Aphid many give birth in lobus cardiacus and blade back shrinkage place, medicament is difficult to be sprayed onto comprehensively, so, remove when requiring to spray medicine thoughtful and careful, also require in medication, to select to have concurrently as far as possible and tag, interior suction, fumigate the triple role agricultural chemicals.Every mu of soup 50～60L that spray is converted at a distance from spray in 7～10 days 1 time, connects and sprays 2～3 times.
Pesticide application is regardless of the performance of agricultural chemicals, and it is residual how application process all causes in tobacco leaf inevitably, finally gets in flue-cured tobacco and the cigarette product, brings healthy hidden danger to the consumer.Along with people's is to the improve of tobacco product quality requirement, and the consumption of agricultural chemicals can more and more be restricted, have in addition the use that can be under an embargo.Can transfer to adopt safer ecological method to carry out integrated control to the control of tobacco insect pest, control aphid method etc. like aphidius gifuensis.This method is tried out many local popularizations.
It is exactly to cultivate special-purpose cigarette seedling earlier that aphidius gifuensis is controlled the aphid method, the cigarette aphid is connect guide to growth and breeding on the cigarette seedling again, introduces aphidius gifuensis when reaching some again; Let aphid cocoon honeybee that it is carried out ovipositing hosts and becomes stiff aphid fast; At last this cigarette seedling that has a stiff aphid is placed or be transplanted to the tobacco land for growing field crops, let aphid cocoon honeybee ovum grow into a large amount of plumage honeybees, the parasitism that the cigarette aphid in field is circulated; It is dead to let cigarette aphid in the cigarette strain ossify, and makes it to endanger the cigarette strain.
The problem that existing aphidius gifuensis is controlled the aphid method is that the land for growing field crops plants that cigarette is grown seedlings and a large amount of numerous honeybee work often conflict mutually in time, and common practices is to build numerous honeybee canopy separately, and its expense accounts for the over half of numerous first honeybee cost; In numerous aphid work, people often only notice the quantity of host plant, ignore the density of host plant; Be the influence that living space grows to aphid, the result is that cigarette seedling density is too high, is unfavorable for the breeding of cigarette aphid; Not only the aphid amount is lower, also can produce a large amount of alatae.
Summary of the invention
The objective of the invention is to propose a kind of Floating seedling and efficiently breed the aphidius gifuensis method; This method can obviously improve the efficient of breeding cigarette aphid, avoids or reduces the emergence aphid, and then improve the speed and the scale of breeding aphidius gifuensis; And the saving cost overcomes the deficiency of prior art with this.
This Floating seedling that the present invention proposes is efficiently bred the aphidius gifuensis method, it is characterized in that it has the following steps:
(1) cultivates host plant cigarette seedling;
(2) connect the numerous aphid of aphid, on go on foot and will plant aphid when cigarette seedling length has 3-4 sheet true leaf and connect and guide on the cigarette seedling, let the cigarette aphid spread breeding voluntarily, and cover the cigarette seedling with fly net;
(3) connect the numerous honeybee of honeybee, become honeybee to connect required aphidius gifuensis and introduce in the fly net, making into honeybee can not escape, and impels into honeybee ovipositing hosts in cigarette aphid body, lets the cigarette aphid form stiff aphid, and then sprouts wings into honeybee.
Carry out in the nursery pond of grow seedlings booth or field hut that (1) step was vacated behind flue-cured tobacco transplanting, do not change nutrient solution in the pond, only, float dish with 162 holes or 200 holes and carry out the alternate row seeding floating seedlings with disinfecting.
(2) step will be planted the aphid leaf with conversion method and will be tiled on the cigarette seedling, appoint the cigarette aphid to spread voluntarily, when on the cigarette seedling 5～8/strain being arranged, and transposition in time.In time cover after connecing aphid, prevent the intrusion of aphidius gifuensis and other insect, influence cigarette aphid propagation with 60 purpose fly nets.Keep 20 ℃～30 ℃ of case temperatures., the aphid amount connects the numerous honeybee of honeybee when reaching 300～350/strain.
(3) goes on foot by honeybee: aphid is inserted required one-tenth honeybee in the cover net than the honeybee amount that connects of 1:100.Under sealing condition, become honeybee not escape, it is parasitic in the cover net, to seek the cigarette aphid, thereby reaches the purpose of efficient parasitism.Under 20 ℃～30 ℃ conditions, through 10～15 days, stiff aphid formed, becomes bee eclosion in a large number.
Fly net is little shed shape, and its height 50～100cm has enough little distance to contact to guarantee cocoon honeybee and cigarette aphid.
One of characteristics of the present invention are to make full use of the existing numerous honeybee of culture facility, significantly practice thrift cost.
Adopted the numerous honeybee required time of Floating seedling only 2 months, and can plant the time difference that cigarette is grown seedlings and grown seedlings with numerous honeybee, make full use of the seedling growth greenhouse vacated behind the flue-cured tobacco transplanting and nutrient solution numerous honeybee of growing seedlings, can reduce numerous honeybee cost to a great extent according to the land for growing field crops.Like numerous honeybee in the booth of growing seedlings, only need on nursery pond, to add a cover the hut of growing seedlings of 60 order fly nets; Like numerous honeybee in the hut of growing seedlings, only need to change fly net into 60 orders and get final product.Planting the cigarette nutrient solution of using of growing seedlings need not change, and can continue after the sterilization to use; Wait to emerge the back according to the tobacco seedling growth situation, topdress by the management expectancy of floating seedlings.If put honeybee it is pressed for time, can connect the numerous aphid of aphid in advance, or strengthen and connect the aphid amount, can shorten about 10 days breeding time.
Two of characteristics of the present invention are to adopt the low-density Floating seedling, have accomplished efficiently to breed host's cigarette aphid.
The key technology of efficient breeding aphidius gifuensis is in unit are, to breed fast a large amount of host aphids.Breed a large amount of host aphids, the one, the fertility of host aphid self is like single female farrowing amount, generation time etc.; The 2nd, enough host plants arranged; The 3rd, need the suitable condition of growing, like suitable temperature, humidity, illumination and living space.In the work of numerous aphid, people often only notice the quantity of host plant, ignore the density of host plant, i.e. living space influence that aphid is grown.If cigarette seedling density is too high, be unfavorable for the breeding of cigarette aphid, not only the aphid amount is lower, also can produce a large amount of alatae, coils 162 strains of growing seedlings as floating with 162 holes, and the single-deck aphid is measured about 2.5 ten thousand.Floating on the dish of 162 holes, alternate row seeding is educated 81 strains of cigarette seedling; During a large amount of numerous honeybees, temperature is that 20 ℃～30 ℃, humidity are under 50%～60% condition, after planting about 30 days; The cigarette seedling connects 5～8/strain of cigarette aphid when 3～4 true leaves are arranged; Through 10 days, single-deck aphid amount reached more than 30,000, promptly every square metre can numerous aphid more than 100,000.With 200 holes float the dish alternate row seeding, 100 strains of growing seedlings, single-deck aphid amount is higher than the summary in 162 holes, difference is little.If reduce seedling density again, like 40 strains, though the individual plant aphid can reach 400, single-deck aphid amount has only about 1.6 ten thousand.
Connect aphid after 10～15 days, the cigarette seedling is big, density is higher, and the cigarette seedling begins to wear out, in addition a large amount of cigarette aphids get food, the cigarette seedling presents the undergrowth state.If continue to appoint the breeding of cigarette aphid, can occur the aphid amount on the contrary and reduce, a large amount of phenomenons that produce of alatae.Should in time connect the numerous honeybee of honeybee this moment.
Three of characteristics of the present invention are that " pressure connects the honeybee method " can efficient parasitic cigarette aphid
Aphidius gifuensis except that the parasitic ability of self, also receives the influence of activity space and host plant density to the parasitism of cigarette aphid.The grow seedlings space of booth is very big, and aphidius gifuensis freely spreads, and is difficult to form the efficient parasitism to the cigarette aphid.So the little shed made from 60 order fly nets covers the cigarette seedling, it is parasitic to make aphidius gifuensis in space limited, sealing, seek the cigarette aphid, becomes a kind of " pressure connects the honeybee method ", has effectively improved parasitic rate, has formed more stiff aphid.Cigarette seedling density is too high, has influenced search, the parasitism of aphidius gifuensis to the cigarette aphid.Float on the dish interlacing 81 strains of growing seedlings in 162 holes, numerous aphid is after 10 days, and by honeybee: aphid is connected into honeybee than the honeybee amount that connects of 1:100, and connects honeybee and add up stiff aphid after 12 days and measure.Adopt " pressure connects the honeybee method ", the stiff aphid amount of individual plant cigarette seedling is 300～350, and the stiff aphid of single-deck is measured nearly 30,000, and promptly every square metre stiff aphid amount reaches more than 100,000.
Adopt " pressure connects the honeybee method ", connect behind the honeybee 15 days and add up the stiff aphid number and the aphid number of living, honeybee: aphid is 98.31% than the stiff aphid rate for 1:50, honeybee: aphid is 95.11% than the stiff aphid rate for 1:100, honeybee: aphid is 90.47% than the stiff aphid rate for 1:150.The one-tenth honeybee that residual cigarette aphid is almost all sprouted wings is parasitic, has effectively avoided the stiff aphid seedling of bulk storage to pass aphid and has passed malicious problem.The promptly suitable honeybee amount that connects is honeybee: aphid is than being 1:100.
Four of characteristics of the present invention are that numerous honeybee comprehensive benefit significantly improves
Low-density Floating seedling breeding aphidius gifuensis technical system; Cultivating host plant, breeding host cigarette aphid and breeding on 3 numerous honeybee links of aphidius gifuensis and all innovate; Utilize the numerous honeybee of existing culture facility; Numerous honeybee cycle was shortened in 2 months, and every square metre stiff aphid and parasitic aphid reach more than 100,000; Management work is easy, and required manpower and materials are less.Be that aphidius gifuensis Ashmaed scale propagation has obtained comprehensive raising at aspects such as time efficiency, unit are efficient, the efficiencies of management, numerous honeybee cost reduces greatly, force urges has been arranged applying of aphidius gifuensis control cigarette aphid technology.
Summary of the invention
In March, 2011, according to such scheme, in the Milei County of applicant unit branch company's east wind triangle the base of growing seedlings implement.With Floating seedling breeding cigarette aphid, with cigarette aphid breeding aphidius gifuensis.Connecing aphid amount, difference through different host density, difference connects honeybee amount, difference and connects honeybee mode and numerous aphid, the research of numerous honeybee time; Formed one and overlapped technology and the method that complete low-density Floating seedling is bred aphidius gifuensis, reached the purpose that improves numerous honeybee efficient, reduces numerous honeybee cost.Respectively as follows:
1. numerous aphid effect of different densities Floating seedling
The dish that floats with 162 holes is grown seedlings, and seedling density is 162 strain/dishes, 81 strain/dishes (broadcasting 1 row at a distance from 1 row), 40 strain/dishes (broadcasting 1 row at a distance from 2 row), when the cigarette seedling has 3～4 true leaves, inserts aphid, and connecing the aphid amount is 2/strain, will plant aphid with writing brush and receive on the cigarette seedling.Each handles 10 dishes, i.e. 10 repetitions.Connect behind the aphid the 10th day, the 15th day, the 20th day, adopt three statistics of parallel sampling method aphid amount.Test temperature is that 15 ℃～24 ℃, humidity are 60%～80%.
The result shows that numerous aphid is after 15 days, and total numerous aphid amount is the highest with 81 strain/dishes, though the height of the 20th day aphid amount than the 15th day, amplification has only 12.10% (seeing table 1).The cigarette seedling density that is numerous aphid is advisable with 81 strain/dishes.
The numerous aphid amount of table 1 different densities Floating seedling (2011.3)
The key technology of efficient breeding aphidius gifuensis is in unit are, to breed fast a large amount of host aphids.Breed a large amount of host aphids, the one, the fertility of host aphid self is like single female farrowing amount, generation time etc.; The 2nd, enough host plants arranged; The 3rd, need the suitable condition of growing, like suitable temperature, humidity, illumination and living space.In the work of numerous aphid, people often only notice the quantity of host plant, ignore the density of host plant, i.e. living space influence that aphid is grown.If cigarette seedling density is too high, be unfavorable for the breeding of cigarette aphid, not only the aphid amount is lower, also can produce a large amount of alatae.Connect aphid after 15 days, the cigarette seedling is big, density is higher, and the cigarette seedling begins to wear out, in addition a large amount of cigarette aphids get food, the cigarette seedling presents the undergrowth state.If continue to appoint the breeding of cigarette aphid, can occur the aphid amount on the contrary and reduce, a large amount of phenomenons that produce of alatae.Should in time connect honeybee, numerous honeybee this moment.
2. the different numerous aphid effects that connect the aphid amount
The dish that floats with 162 holes is grown seedlings, and seedling density is 81 strain/dishes (broadcast at a distance from 1 row 1 capable), inserts aphid constantly when the cigarette seedling has 3～4 true leaves, connects the aphid amount and be 3/strain, 5/strain, 8/strain, 10/strain, will plant aphid with writing brush and receive on the cigarette seedling.Each handles 10 dishes, i.e. 10 repetitions.Connect behind the aphid the 10th day, the 15th day, the 20th day, adopt three statistics of parallel sampling method aphid amount.Test temperature is that 18 ℃～27 ℃, humidity are 60%～80%.
The result shows; Connect aphid amount and be 5/strain, 8/strain, 10/strain, numerous aphid effect of 15 days of numerous aphid is better, from connecing analysis-by-synthesis such as aphid amount, numerous aphid time and numerous aphid amount; Be advisable with interior to connect 5～8/strain of aphid amount, numerous aphid 15 days, numerous aphid after 20 days the aphid amount sharply descend on the contrary; The proliferative amount of cigarette aphid can not improve when connecing aphid above 8/strain, can reduce (seeing table 2) on the contrary.
Table 2 difference connects numerous aphid amount (2011.4) of aphid amount
3. difference connects numerous honeybee effect that honeybee is measured, difference connects the honeybee mode
The dish that floats with 162 holes is grown seedlings, and seedling density is 81 strain/dishes (broadcasting 1 row at a distance from 1 row), when the cigarette seedling has 3～4 true leaves, inserts aphid, and connecing the aphid amount is 5/strain, will plant aphid with writing brush and receive on the cigarette seedling.Connect behind the aphid the 12nd day, average aphid amount reaches 300/ strain when above, and by honeybee: aphid is inserted into honeybee than 1:50,1:100,1:150, connects to cover with 60 order fly nets respectively behind the honeybee to float dish, appoints into honeybee searching cigarette aphid parasitism.Simultaneously, by honeybee: after aphid connects honeybee than the honeybee amount that connects for 1:100, need not 60 order fly nets cover and float dish and contrast.Each handles 10 dishes, i.e. 10 repetitions.Connect behind the honeybee the 10th day, the 15th day, the 20th day, adopt three statistics of parallel sampling method aphid amount.Test temperature is that 21 ℃～30 ℃, humidity are 60%～80%.
The result shows, adds a cover the numerous honeybee of fly net after 10 days, stiff aphid that each is handled and parasitic aphid amount reach 2.5 ten thousand/more than the dish (310/strain), promptly every square metre more than 100,000; Numerous honeybee is after 15 days, stiff aphid that each is handled and parasitic aphid reach 30,000/more than the dish, promptly every square metre more than 130,000; With honeybee: aphid is than being the highest of 1:100, and honeybee: aphid is than taking second place for 1:150; Honeybee: aphid is than being the lower of 1:50.Numerous honeybee is stiff aphid and all obviously decline (seeing table 3) of parasitic aphid after 20 days.Its reason is that to connect honeybee too high, and a large amount of cigarette aphids are by parasitism, and the aphid amount increases few, the later stage stiff aphid and parasitic aphid just few; The suitable honeybee amount that connects, though early stage, parasitic rate was lower, the cigarette aphid can continue propagation, the new parasitic again cigarette aphid of one-tenth honeybee that sprouts wings after 10 days, thus total stiff aphid and parasitic aphid are obviously increased; Along with wearing out of cigarette seedling, the mortality of cigarette aphid, stiff aphid comes off in a large number, so stiff aphid reduces in a large number after 20 days.
The different honeybees of table 3: aphid ratio and the bee yield of adding a cover fly net (2011.5)
Do not add a cover fly net, honeybee after connecing honeybee: aphid is than for 1:100, and in the identical time, its stiff aphid amount and stiff aphid rate all are starkly lower than adds a cover fly net.Promptly under condition limited, sealing, connect honeybee, numerous honeybee, can obviously improve numerous honeybee efficient.
The bee yield of the open numerous honeybee of table 4 (2011.5)
Annotate: stiff aphid amount contains parasitic aphid in the table
Aphidius gifuensis except that the parasitic ability of self, also receives the influence of activity space and host plant density to the parasitism of cigarette aphid.The grow seedlings space of booth is very big, and aphidius gifuensis freely spreads, and is difficult to form the efficient parasitism to the cigarette aphid.So the little shed made from 60 order fly nets covers the cigarette seedling, it is parasitic to make aphidius gifuensis in space limited, sealing, seek the cigarette aphid, becomes a kind of " pressure connects the honeybee method ", has effectively improved parasitic rate, has formed more stiff aphid.
4. make full use of the existing numerous honeybee of culture facility, numerous honeybee remarkable benefit improves
The land for growing field crops plants cigarette and grows seedlings and a large amount of numerous honeybee work often conflict mutually in time, and common practices is to build numerous honeybee canopy separately, and its expense accounts for the over half of numerous first honeybee cost.Adopted the numerous honeybee required time of Floating seedling only 2 months, and can plant the time difference that cigarette is grown seedlings and grown seedlings with numerous honeybee, make full use of the seedling growth greenhouse vacated behind the flue-cured tobacco transplanting and nutrient solution numerous honeybee of growing seedlings, can reduce numerous honeybee cost to a great extent according to the land for growing field crops.Like numerous honeybee in the booth of growing seedlings, only need on nursery pond, to add a cover the hut of growing seedlings of 60 order fly nets; Like numerous honeybee in the hut of growing seedlings, only need to change fly net into 60 orders and get final product.Planting the cigarette nutrient solution of using of growing seedlings need not change, and can continue after the sterilization to use; Wait to emerge the back according to the tobacco seedling growth situation, topdress by the management expectancy of floating seedlings.
Floating seedling is efficiently bred the aphidius gifuensis technical system; Cultivating host plant, breeding host cigarette aphid and breeding on 3 numerous honeybee links of aphidius gifuensis and all innovate; Utilize the numerous honeybee of existing culture facility; Numerous honeybee cycle was shortened in 2 months, and every square metre stiff aphid and parasitic aphid reach more than 100,000; Do not have links such as transplanting and cultivate the cigarette strain, management work is easy, and required manpower and materials are less.Be that aphidius gifuensis Ashmaed scale propagation has obtained comprehensive raising at aspects such as time efficiency, unit are efficient, the efficiencies of management, numerous honeybee cost reduces greatly, force urges has been arranged applying of aphidius gifuensis control cigarette aphid technology.
Claims (6)
1. a Floating seedling is efficiently bred the aphidius gifuensis method, it is characterized in that it has the following steps:
(1) cultivates host plant cigarette seedling;
(2) connect the numerous aphid of aphid, on go on foot and will plant aphid when cigarette seedling length has 3～4 true leaves and connect to guide to and let the cigarette aphid spread breeding voluntarily on the cigarette seedling, and cover the cigarette seedling with fly net;
(3) connect the numerous honeybee of honeybee, become honeybee to connect required aphidius gifuensis and introduce in the fly net, making into honeybee can not escape, and impels into honeybee ovipositing hosts in cigarette aphid body, lets the cigarette aphid form stiff aphid, and then sprouts wings into honeybee.
2. efficiently breed the aphidius gifuensis method according to the said Floating seedling of claim 1; It is characterized in that carrying out in the nursery pond of (1) grow seedlings booth or the field hut behind flue-cured tobacco transplanting, vacated of step; Do not change nutrient solution in the pond; Only, float dish with 162 holes or 200 holes and carry out the alternate row seeding floating seedlings with disinfecting.
3. efficiently breed the aphidius gifuensis method according to the said Floating seedling of claim 1; It is characterized in that (2) step will plant the aphid leaf with conversion method and be tiled on the cigarette seedling; Appoint the cigarette aphid to spread voluntarily, when on the cigarette seedling 5～8/strain being arranged, transposition in time; In time cover after connecing aphid, keep 20 ℃～30 ℃ of case temperatures with 60 purpose fly nets.
4. efficiently breed the aphidius gifuensis method according to the said Floating seedling of claim 1, it is characterized in that (3) goes on foot by honeybee: aphid is inserted required one-tenth honeybee in the cover net than the honeybee amount that connects of 1:100.
5. efficiently breed the aphidius gifuensis method according to claim 1 or 4 said Floating seedlings, it is characterized in that when the aphid amount reaches 300～350/strain, connecing the numerous honeybee of honeybee.
6. efficiently breed the aphidius gifuensis method according to claim 1 or 3 said Floating seedlings, it is characterized in that fly net is little shed shape, its height 50～100cm.
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| CN106172258A (en) * | 2016-08-15 | 2016-12-07 | 云南省烟草公司红河州公司 | A kind of field dissipates the method for aphid on stiff aphid Seedling preventing and treating Nicotiana tabacum L. or other crop |
| CN106613188A (en) * | 2016-12-02 | 2017-05-10 | 湖北省农业科学院中药材研究所 | Efficient reproduction method for valeriana officinalis L. |
| CN107371764A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司楚雄州公司 | A kind of method that wet tobacco seedlings breeding cigarette aphid and aphidius gifuensis are floated using regeneration |
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| CN110771577B (en) * | 2019-11-18 | 2021-09-24 | 西南林业大学 | High-density rapid breeding method for stiff aphids of aphidius gifuensis ashmeads |
| CN114097712A (en) * | 2021-11-24 | 2022-03-01 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Royal aphidiidae breeding device and method |
| CN114097712B (en) * | 2021-11-24 | 2023-08-15 | 宁夏农林科学院植物保护研究所（宁夏植物病虫害防治重点实验室） | Device and method for breeding aphidius gifuensis |
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