CN101664017A - Method for propagating sulzer braconid flies - Google Patents
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- CN101664017A CN101664017A CN200910095036A CN200910095036A CN101664017A CN 101664017 A CN101664017 A CN 101664017A CN 200910095036 A CN200910095036 A CN 200910095036A CN 200910095036 A CN200910095036 A CN 200910095036A CN 101664017 A CN101664017 A CN 101664017A
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Abstract
The invention discloses a method for propagating sulzer braconid flies. The method comprises the following steps: firstly, culturing a host plant of tobaccos, then propagating sulzers and afterwards propagating the sulzer braconid flies by the sulzers. By propagating the flies in batches, the multi-generation repeated propagation of the sulzer braconid flies is avoided, the generation quantity ofthe hyperparasite flies of the sulzer braconid flies is reduced, and the survival capability of the sulzer braconid flies in the field is enhanced. The propagation time and quantity of the sulzer braconid flies can be flexibly regulated and controlled according to the actual condition of the field, and different requirements on the sulzer braconid flies under different situations are met. The emergence rate of the stiff aphids of the sulzer braconid flies propagated by the invention is more than 90%, the male-female ratio is 0.8-1.0:1, and the utilization rate of the stiff aphids or the adultflies is more than 80%.
Description
Technical field
The invention belongs to damage by disease and insect biological control technical field, relate to the utilization of natural enemy insect, be specifically related to a kind of method for propagating sulzer braconid flies.
Background technology
In recent years, the application development of natural enemy insect is very fast both at home and abroad, and breeding and the release of oophagous trichogrammae, predation mite become the model that China's natural enemy insect utilizes, and the utilization of natural enemy insect has developed into a kind of important measures of crop pests control in the modern agriculture.Aphidius gifuensis (Apidius gifuensis Ashmead) is a kind of endoparasitism honeybee of special parasitic aphid, it is the important parasite of cigarette aphid, all generally take place in each cigarette district of China, natural control to the cigarette aphid is stronger, in the vega aphidius gifuensis to the natural parasitic rate of cigarette aphid usually between 20%～60%, Gao Shikeda Zhao 89.16%[Wanyuan, Ding Chuiping. aphidius gifuensis biology and application study thereof. zoological research, 1980,1 (3): 405 415; Chen Jiahua, official's treasured is refined, Zhang Yuzhen. the interrelationship study of aphidius gifuensis and cigarette aphid. and Chinese tobacco journal, 1996,3 (1): 8-12.].
As a kind of important parasite of cigarette aphid, aphidius gifuensis has the comparatively wide prospect of utilizing aspect the biological control of cigarette aphid, is the object that China biological control worker pays close attention to always.At present, the host that the artificial propagation aphidius gifuensis adopts mostly is the cigarette aphid, and breeding cigarette aphid is adopted cigarette seedling or Turnip Sprouts more, about the scholar has done more research to biology and ecological characteristics, the indoor propagation method of aphidius gifuensis.On used in the field, Yuxi, Yunnan Yan Qu applied the more than ten years, formed comparatively ripe numerous honeybee technology (aphidius gifuensis Ashmaed scale propagation technique CN 101455190).But in the existing propagation technique,, cause numerous honeybee efficient, quality instability easily, influence numerous honeybee effect of aphidius gifuensis aspect the ins and outs of cigarette aphid kind aphid and the selection of aphidius gifuensis honeybee kind, numerous honeybee, remaining in some problems.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, a kind of honeybee efficient easy and simple to handle, numerous and stay-in-grade aphidius gifuensis propagation method are provided.
Purpose of the present invention is achieved by the following technical programs.
A kind of method for propagating sulzer braconid flies comprises: cultivate earlier the host plant tobacco, again breed the cigarette aphid, utilize cigarette aphid breeding aphidius gifuensis then, concrete steps are as follows:
1, the cultivation of host plant tobacco
A. feel the selection of aphid tobacco bred: select the tobacco bred of the low sense cigarette aphid of proline content height and nicotine content, mildew-resistance standby;
B. grow seedlings: as seedling rearing room, in seedling rearing room, tobacco vernalization pelleted seed is educated to Cheng Miao with 22 ± 5 ℃, humidity 60%～70%, the greenhouse that has fly net or warm canopy;
C. transplant: as cigarette seedling raising chamber, the cigarette transplantation of seedlings bred to flowerpot, was cultivated 10～15 days in cigarette seedling raising chamber with 20 ℃～25 ℃, humidity 50%～60%, the greenhouse that has fly net or warm canopy;
In the step 1, the preferred floating seedlings method of the seedling-cultivating method of described tobacco seed-coating kind, husky training seedling method or moistening seedling method.
Diameter 8cm～10cm, high 15～20cm at the bottom of the basin mouth diameter 12～16cm of described flowerpot, the basin.
2, the breeding of cigarette aphid
A. the seed selection of cigarette aphid: with 20 ℃～25 ℃ of temperature, humidity 50%～60%, the greenhouse that has fly net or warm canopy as kind of an aphid seed selection chamber, gather kind of an aphid, raise 2～3 generations with the cigarette strain of transplanting, cultivating 10～15 days, the selection cigarette aphid that reproduction speed is fast, vitality is strong is as kind of an aphid;
B. connect aphid:, involve the cigarette of transplanting, cultivated 10～15 days in a criminal case the basin immigration and connect the aphid chamber as connecing the aphid chamber with 20 ℃～25 ℃ of temperature, humidity 50%～60%, the greenhouse that has fly net or warm canopy, adopt the mode that manually connects aphid on every strain cigarette seedling, to connect the cigarette aphid; Cultivate 2～3 day after whether inspection affirmation cigarette aphid survive if aphid is received on the middle and lower part blade of cigarette seedling equably with aptery one-tenth aphid picking IV age when connecing aphid; If survive quantity not sufficient, can mend and connect;
C. the cigarette aphid is expanded numerous: with 20 ℃～25 ℃ of temperature, humidity 50%～60%, the greenhouse that has fly net or warm canopy as numerous aphid chamber, involve basin in a criminal case and move into numerous aphid chamber connecing cigarette behind the aphid, freely bred 10～20 days, the cigarette aphid is expanded needs to add that shading screen is sun-proof during numerous, nature or electric fan ventilate, prevent that cigarette fleahopper, aphidius gifuensis etc. from importing the breeding of inhibition cigarette aphid into, prevents the generation of aphid mould, tobacco powdery mildew etc.;
Kind aphid described in the step 2 is the cigarette aphid that survives the winter on radish, curly dock, shepherd's purse, Hairy Bittercress, rorippa indica, butch clover or the peach.
Describedly manually connect aphid and be divided into 10～20/strain of 2 numerous at a slow speed aphids of type: I, 30～50/strain of the numerous aphid of II middling speed by the quantity that every strain cigarette inserts the cigarette aphid.
During the 2nd numerous aphid, plant directly picking in numerous aphid chamber of aphid.
3, the breeding of aphidius gifuensis
A. the selection of honeybee kind: select individual bigger aphidius gifuensis and become honeybee, as stock honeybee kind;
B. it is numerous with expansion to connect honeybee: expand numerous chamber with 20 ℃～25 ℃ of temperature, humidity 60%～70%, the greenhouse that has fly net or warm canopy as connecing honeybee, to be with the aphid cigarette to involve basin in a criminal case moves into and to connect honeybee and expand numerous chamber, manually connect honeybee, aphidius gifuensis is grown and is formed stiff aphid after 8～10 days, and stiff aphid turns into into honeybee after 5～6 days.
In the step 3, during first numerous honeybee, on Winter-Spring cigarette or other crop, weeds, gather the stiff aphid of aphidius gifuensis, be placed in the glass container that a usefulness 60 order nylon wires seal, and add the cotton ball dip in 5% hydromel or white sugar water, keep cotton balls moist, treat the stiff aphid emergence of aphidius gifuensis 80% or more after, select individual bigger aphidius gifuensis and become honeybee, as the honeybee kind.
During the 2nd numerous honeybee, the honeybee kind is picking in numerous honeybee chamber directly.
Aphidius gifuensis expands should prevent the hyperparasitism honeybee during numerous, will accumulate in pooter to connect honeybee and expand hyperparasitism honeybee sucking-off on the phototropic face wall of numerous chamber.
Describedly manually connect honeybee and be divided into 2 types by the quantitative proportion of queen bee and cigarette aphid: the quick numerous honeybee queen bee of an I generation: the cigarette aphid is 1: 40～60, and the numerous honeybee queen bee of II two generations middling speed: the cigarette aphid is 1: 100～200.
Describedly manually connect honeybee and be divided into 2 type: I by cigarette aphid breeding quantity and measure when breeding 2000～3000/strain when the individual plant aphid, by queen bee: aphid is that 1: 100～200 the honeybee amount that connects connects honeybee, allow aphidius gifuensis in greenhouse or warm canopy through the numerous honeybee of two generation middling speeds; II breeds 5000～6000/strain when individual plant aphid amount, and by queen bee: aphid is that 1: 40～60 the honeybee amount that connects connects honeybee, allows aphidius gifuensis pass through the quick numerous honeybee of a generation in greenhouse or warm canopy.
With respect to prior art, the present invention has the following advantages:
1, in whole numerous honeybee process, tobacco plant finishes to numerous honeybee from connecing the cigarette aphid, and the proline content of tobacco leaf is higher relatively, and the nicotine cumulant is few, for the breeding of cigarette aphid provides good food, has avoided the formation of alatae.
2, in numerous honeybee process, seedling rearing room, cigarette seedling raising chamber, plant aphid seed selection chamber, connect aphid chamber, numerous aphid chamber, connecing honeybee, to expand numerous chamber relatively independent, can guarantee numerous honeybee continue carry out, avoided mixing of cigarette strain, cigarette aphid, aphidius gifuensis, kept cigarette strain, cigarette aphid, aphidius gifuensis population independently separately.Grew seedlings in 7～10 days as every interval, can realize long-term numerous honeybee.This numerous in batches honeybee has avoided many generations of aphidius gifuensis to breed repeatedly, has reduced the generation quantity of aphidius gifuensis hyperparasitism honeybee, has strengthened aphidius gifuensis field survival ability.
3, grow seedlings, numerous aphid, numerous honeybee scale can be taken place with field cigarette aphid, the situation of development is undertaken by grow seedlings quantity what, the organic assembling of numerous aphid type, numerous honeybee type that numerous honeybee scale is regulated, control, regulate the breeding time and the breeding amount of control aphidius gifuensis, satisfied under the different situations different demands aphidius gifuensis.
4, the stiff aphid eclosion rate of the aphidius gifuensis of the present invention breeding 90% or more, male and female are than 0.8～1.0: 1, and the availability of stiff aphid or one-tenth honeybee is more than 80%.
Embodiment:
By specific embodiment given below, can further be well understood to the present invention.But they are not limitation of the invention.
A situation arises determines aphidius gifuensis bulk storage date, bulk storage area and quantity according to control area and cigarette aphid over the years, grows seedlings usually and cultivate the quantity that the quantity of cigarette strain, numerous aphid and numerous honeybee compound mode have determined the aphidius gifuensis bulk storage.During the bulk storage of field, early stage, the middle and later periods was based on the quick numerous honeybee of a generation based on the numerous honeybee of two generation middling speeds.
Embodiment 1
Adopt the numerous honeybee of two generation middling speeds, before normal field-transplanting, carried out in 30～45 days, specific as follows:
1, the cultivation of host plant tobacco
Grow seedlings: select K326 vernalization pelleted seed (China invention ZL 0335483.1) for use, adopt floating seedlings method (China invention ZL 03108979.8), in 20 ℃～25 ℃, humidity 60%～70%, the greenhouse that has fly net or warm canopy (as seedling rearing room), grow seedlings, to Cheng Miao.
Transplant: the tobacco of breeding is transplanted in the flowerpot of diameter 8cm～10cm, high 15～20cm at the bottom of basin mouth diameter 12～16cm, the basin, cultivation is 12 days in 20 ℃～25 ℃, humidity 50%～60%, the greenhouse that has fly net or warm canopy (as cigarette seedling raising chamber).
2, the breeding of cigarette aphid
The breeding of cigarette aphid comprises the seed selection of cigarette aphid, connects aphid, the cigarette aphid is expanded numerous.
The seed selection of cigarette aphid: plant aphid and select the cigarette aphid that survives the winter on the radish, adopt back the back in 20 ℃～25 ℃, humidity 50%～60%, the greenhouse that has fly net or warm canopy (as kind of an aphid seed selection chamber) with transplant, 12 days cigarette strain (band basin) of cultivation, 2～3 generations of raising.The selection cigarette aphid that reproduction speed is fast, vitality is strong is as kind of an aphid.
Connect the cigarette aphid: the cigarette that will transplant, cultivate 12 days involves 20 ℃～25 ℃ of basin immigrations, humidity 50%～60%, the greenhouse that has fly net or warm canopy (as connecing the aphid chamber) in a criminal case, adopts the mode that manually connects aphid to connect 10 of cigarette aphids (the numerous aphid of middling speed) on every strain cigarette seedling.Cultivate 2～3 day if aphid becomes aphid to receive equably on the middle and lower part blade of cigarette seedling with aptery picking IV age when connecing aphid, and whether affirmation cigarette aphid survives, as survive quantity not sufficient and can mend and connect.
It is numerous that the cigarette aphid is expanded: will connect cigarette behind the aphid and involve basin in a criminal case and move to 20 ℃～25 ℃, humidity 50%～60%, the greenhouse that has fly net or warm canopy (as numerous aphid chamber), and allow the cigarette aphid freely breed on the cigarette seedling 15 days.Need to add sun-proof, the gravity-flow ventilation of shading screen during the expansion of cigarette aphid is numerous, prevent that cigarette fleahopper, aphidius gifuensis etc. from importing the breeding of inhibition cigarette aphid into, prevents the flight of cigarette aphid, the generation of control aphid mould, tobacco powdery mildew etc.End during 2000～3000 of every strain breeding cigarette aphids.
3, the breeding of aphidius gifuensis
The breeding of aphidius gifuensis comprises the seed selection of honeybee kind, connects honeybee and expands numerous.
The seed selection of honeybee kind: on Winter-Spring cigarette or other crop, weeds, gather the stiff aphid of aphidius gifuensis, be placed on that a usefulness 60 order nylon wires seal glass container in, and add the cotton ball that dips in 5% hydromel or white sugar water (as the food of aphidius gifuensis), keep cotton balls moist; After treating that the stiff aphid of aphidius gifuensis more than 80% sprouts wings, select individual bigger aphidius gifuensis and become honeybee, directly as stock honeybee kind or to be used for the expansion of honeybee kind numerous.
It is numerous with expansion to connect honeybee: will involve 20 ℃～25 ℃ of basin immigrations, humidity 60%～70%, the greenhouse that has fly net or warm canopy (expanding numerous chamber as connecing honeybee) in a criminal case with the aphid cigarette, by queen bee: the cigarette aphid is 1: 100～200 manually to connect honeybee, allows aphidius gifuensis grow and forms stiff aphid (numerous honeybee of two generations).Aphidius gifuensis needs 8～10 days from parasitism to the time that forms stiff aphid, and stiff aphid needs 5～6 days to turning into into honeybee.Aphidius gifuensis expands needs anti-hyperparasitism honeybee during numerous, can utilize pooter will accumulate in hyperparasitism honeybee sucking-off on greenhouse or the warm canopy phototropic face wall.
During the numerous honeybee of two generation middling speeds, because the aphidius gifuensis ratio is little when initially connecing honeybee, aphidius gifuensis also has and is continued breeding by the cigarette aphid of parasitism in parasitic cigarette aphid, and aphidius gifuensis just can breed for two generations like this.
The numerous honeybee of this two generation middling speeds can be applicable to field cigarette aphid and puts honeybee continuously when beginning sporadicly to take place, and can strengthen the honeybee amount of putting one by one.
Embodiment 2
Adopt the quick numerous honeybee of a generation, specific as follows:
1, K326 vernalization pelleted seed (China invention ZL0335483.1) is selected in the cultivation of host plant tobacco for use, adopts husky training seedling method (China invention ZL 200510048691.3), transplants the back and cultivates 15 days.All the other are with embodiment 1.
2, the breeding of cigarette aphid
The seed selection of cigarette aphid: plant directly picking in numerous aphid chamber of aphid.
Connect the cigarette aphid: the cigarette that will transplant, cultivate 15 days involves 20 ℃～25 ℃ of basin immigrations, humidity 50%～60%, the greenhouse that has fly net or warm canopy (as connecing the aphid chamber) in a criminal case, adopts the mode of 40/strain of quick numerous aphid to connect the cigarette aphid on every strain cigarette seedling.Cultivate 2～3 day if aphid becomes aphid to receive equably on the middle and lower part blade of cigarette seedling with aptery picking IV age when connecing aphid, and whether affirmation cigarette aphid survives, as survive quantity not sufficient and can mend and connect.
The cigarette aphid is expanded numerous: with embodiment 1,5000～6000 of every strain breeding cigarette aphids are ended.
3, the breeding of aphidius gifuensis
The seed selection of honeybee kind: direct picking in numerous honeybee chamber.
Connect honeybee, expand numerous: involve band aphid cigarette in a criminal case 20 ℃～25 ℃ of basin immigrations, humidity 60%～70%, the greenhouse that has fly net or warm canopy (expanding numerous chamber) as connecing honeybee, adopt by queen bee: the cigarette aphid is that quick numerous honeybee mode of 1: 50 manually connects honeybee, allows aphidius gifuensis grow and forms stiff aphid.Aphidius gifuensis needs 8～10 days from parasitism to the time that forms stiff aphid, and stiff aphid needs 5～6 days to turning into into honeybee.Aphidius gifuensis expands needs anti-hyperparasitism honeybee during numerous, can utilize pooter will accumulate in hyperparasitism honeybee sucking-off on greenhouse or the warm canopy phototropic face wall.
During the quick numerous honeybee of a generation, because aphidius gifuensis population density height when initially connecing honeybee, ratio are big, aphidius gifuensis reached the parasitism to 90% above cigarette aphid in 2～3 days, and aphidius gifuensis passes through a generation and breeds like this, just can obtain the aphidius gifuensis of larger amt.Plant cigarette 400 basins as 50 square metres every warm canopy,, can guarantee after connecing honeybee to breed 2,000,000 stiff aphids (5000 stiff aphid/strain * 400 strains) or to become honeybee in 13～15 days according to above numerous honeybee method.
The quick numerous honeybee of a this generation can be applicable to the field cigarette aphid high-incidence season, and putting honeybee continuously can realize by numerous honeybee of 7～10 days interval.
Embodiment 3
Repeat embodiment 1, following difference arranged: during the host plant tobacco seedling, adopt moistening seedling method [Liu Tianyi, Huang Yilan, Lai Luxiang etc. the moistening Study on Seedling Cultivation Technique of flue-cured tobacco. Chinese tobacco science .2009,29 (5): 22-26.].
Embodiment 4～6
Repeat embodiment 1, following difference is arranged: plant the cigarette aphid that survives the winter on aphid selection curly dock, shepherd's purse or the Hairy Bittercress.
Embodiment 7～9
Repeat embodiment 1, following difference is arranged: plant the cigarette aphid that survives the winter on aphid selection wrinkle rorippa indica, butch clover or the peach.
Claims (10)
1, a kind of method for propagating sulzer braconid flies comprises: cultivate earlier the host plant tobacco, again breed the cigarette aphid, utilize cigarette aphid breeding aphidius gifuensis then, concrete steps are as follows:
(1) cultivation of host plant tobacco
A. feel the selection of aphid tobacco bred: select the tobacco bred of the low sense cigarette aphid of proline content height and nicotine content, mildew-resistance standby;
B. grow seedlings: as seedling rearing room, in seedling rearing room, tobacco vernalization pelleted seed is educated to Cheng Miao with 22 ± 5 ℃, humidity 60%～70%, the greenhouse that has fly net or warm canopy;
C. transplant: as cigarette seedling raising chamber, the cigarette transplantation of seedlings bred to flowerpot, was cultivated 10～15 days in cigarette seedling raising chamber with 20 ℃～25 ℃, humidity 50%～60%, the greenhouse that has fly net or warm canopy;
(2) breeding of cigarette aphid
A. the seed selection of cigarette aphid: with 20 ℃～25 ℃ of temperature, humidity 50%～60%, the greenhouse that has fly net or warm canopy as kind of an aphid seed selection chamber, gather kind of an aphid, raise 2～3 generations with the cigarette strain of transplanting, cultivating 10～15 days, the selection cigarette aphid that reproduction speed is fast, vitality is strong is as kind of an aphid;
B. connect aphid:, involve the cigarette of transplanting, cultivated 10～15 days in a criminal case the basin immigration and connect the aphid chamber as connecing the aphid chamber with 20 ℃～25 ℃ of temperature, humidity 50%～60%, the greenhouse that has fly net or warm canopy, adopt the mode that manually connects aphid on every strain cigarette seedling, to connect the cigarette aphid; Cultivate and 2～3 day after check whether affirmation cigarette aphid survive if aphid and aptery one-tenth aphid are received on the middle and lower part blade of cigarette seedling equably picking IV age when connecing aphid;
C. the cigarette aphid is expanded numerous: with 20 ℃～25 ℃ of temperature, humidity 50%～60%, the greenhouse that has fly net or warm canopy as numerous aphid chamber, involve basin in a criminal case and move into numerous aphid chamber connecing cigarette behind the aphid, freely bred 10～20 days, the cigarette aphid is expanded needs to add that shading screen is sun-proof during numerous, nature or electric fan ventilate, prevent that cigarette fleahopper, aphidius gifuensis etc. from importing the breeding of inhibition cigarette aphid into, prevents the generation of aphid mould, tobacco powdery mildew;
(3) breeding of aphidius gifuensis
A. the selection of honeybee kind: select individual bigger aphidius gifuensis and become honeybee, as stock honeybee kind;
B. it is numerous with expansion to connect honeybee: expand numerous chamber with 20 ℃～25 ℃ of temperature, humidity 60%～70%, the greenhouse that has fly net or warm canopy as connecing honeybee, to be with the aphid cigarette to involve basin in a criminal case moves into and to connect honeybee and expand numerous chamber, manually connect honeybee, aphidius gifuensis is grown and is formed stiff aphid after 8～10 days, and stiff aphid turns into into honeybee after 5～6 days.
2, method for propagating sulzer braconid flies according to claim 1 is characterized in that: the seedling-cultivating method of the tobacco seed-coating kind described in the step (1) is floating seedlings method, husky training seedling method or moistening seedling method.
3, method for propagating sulzer braconid flies according to claim 1 is characterized in that: the kind aphid described in the step (2) is the cigarette aphid that survives the winter on radish, curly dock, shepherd's purse, Hairy Bittercress, rorippa indica, butch clover or the peach.
4, method for propagating sulzer braconid flies according to claim 1 is characterized in that: the aphid that manually connects described in the step (2) is divided into 10～20/strain of 2 numerous at a slow speed aphids of type: I, 30～50/strain of the numerous aphid of II middling speed by the quantity that every strain cigarette inserts the cigarette aphid.
5, method for propagating sulzer braconid flies according to claim 1 is characterized in that: when aphid is planted in the 2nd breeding, plant directly picking in numerous aphid chamber of aphid.
6, method for propagating sulzer braconid flies according to claim 1, it is characterized in that: in the step (3), during first numerous honeybee, on Winter-Spring cigarette or other crop, weeds, gather the stiff aphid of aphidius gifuensis, be placed in the glass container that a usefulness 60 order nylon wires seal, and add the cotton ball that dips in 5% hydromel or white sugar water, keep cotton balls moist, after treating the stiff aphid emergence of aphidius gifuensis more than 80%, select individual bigger aphidius gifuensis and become honeybee, as the honeybee kind.
7, method for propagating sulzer braconid flies according to claim 6 is characterized in that: during the 2nd numerous honeybee, the honeybee kind is picking in numerous honeybee chamber directly.
8, method for propagating sulzer braconid flies according to claim 1 is characterized in that: expand at the described aphidius gifuensis of step (3) and should prevent the hyperparasitism honeybee during numerous, will accumulate in pooter and connect honeybee and expand hyperparasitism honeybee sucking-off on the phototropic face wall of numerous chamber.
9, method for propagating sulzer braconid flies according to claim 1, it is characterized in that: step (3) is described manually to be connect honeybee and be divided into 2 types by the quantitative proportion of queen bee and cigarette aphid: the quick numerous honeybee queen bee of an I generation: the cigarette aphid is 1: 40～60, and II is for the numerous honeybee queen bee of middling speed: the cigarette aphid is 1: 100～200.
10, method for propagating sulzer braconid flies according to claim 1, it is characterized in that: step (3) is described manually to be connect honeybee and is divided into 2 type: I by cigarette aphid breeding quantity and measures when breeding 2000～3000/strain when the individual plant aphid, by queen bee: aphid is that 1: 100～200 the honeybee amount that connects connects honeybee, allow aphidius gifuensis in greenhouse or warm canopy through the numerous honeybee of two generation middling speeds; II breeds 5000～6000/strain when individual plant aphid amount, and by queen bee: aphid is that 1: 40～60 the honeybee amount that connects connects honeybee, allows aphidius gifuensis pass through the quick numerous honeybee of a generation in greenhouse or warm canopy.
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