CN103478083A - Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae - Google Patents
Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae Download PDFInfo
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- CN103478083A CN103478083A CN201310449593.5A CN201310449593A CN103478083A CN 103478083 A CN103478083 A CN 103478083A CN 201310449593 A CN201310449593 A CN 201310449593A CN 103478083 A CN103478083 A CN 103478083A
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- 241001124076 Aphididae Species 0.000 title claims abstract description 279
- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 133
- 238000009395 breeding Methods 0.000 title claims abstract description 63
- 230000001488 breeding effect Effects 0.000 title claims abstract description 61
- 241000257303 Hymenoptera Species 0.000 title abstract 5
- 241000721621 Myzus persicae Species 0.000 title abstract 5
- 241000196324 Embryophyta Species 0.000 claims abstract description 120
- 201000010099 disease Diseases 0.000 claims abstract description 18
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 13
- 241000220259 Raphanus Species 0.000 claims abstract description 8
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims abstract description 8
- 230000002265 prevention Effects 0.000 claims abstract description 6
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 235000013311 vegetables Nutrition 0.000 claims abstract description 5
- 241000180219 Sitobion avenae Species 0.000 claims abstract description 4
- 241000256844 Apis mellifera Species 0.000 claims description 81
- 235000019504 cigarettes Nutrition 0.000 claims description 81
- 230000003203 everyday effect Effects 0.000 claims description 20
- 238000005286 illumination Methods 0.000 claims description 20
- 241000219193 Brassicaceae Species 0.000 claims description 14
- 238000001784 detoxification Methods 0.000 claims description 12
- 230000007613 environmental effect Effects 0.000 claims description 12
- 239000003337 fertilizer Substances 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 230000003071 parasitic effect Effects 0.000 claims description 9
- 210000003608 fece Anatomy 0.000 claims description 8
- 239000010871 livestock manure Substances 0.000 claims description 8
- 238000010899 nucleation Methods 0.000 claims description 8
- 235000013601 eggs Nutrition 0.000 claims description 6
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- 241000238631 Hexapoda Species 0.000 claims description 4
- 241000382353 Pupa Species 0.000 claims description 4
- 238000012271 agricultural production Methods 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- 230000024241 parasitism Effects 0.000 claims description 4
- 230000001902 propagating effect Effects 0.000 claims description 4
- 238000000746 purification Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000032669 eclosion Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 230000017448 oviposition Effects 0.000 abstract description 2
- 241001600408 Aphis gossypii Species 0.000 abstract 1
- 235000009508 confectionery Nutrition 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 101100248253 Arabidopsis thaliana RH40 gene Proteins 0.000 description 5
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
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Abstract
Disclosed is a large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae. The yellow-green myzuspersicae with large bodies serve as host aphids of aphidiusgifuensis, and parent aphid disease-resistant leafy cruciferous green vegetables, radishes or sweet blue flowers serve as host plants of the yellow-green myzuspersicae, so that the mummified aphids and the adult bees of the aphidiusgifuensis are artificially bred on a large scale. The method solves the problems that the bodies of the host aphids are small, the mummified aphids are difficultly collected, the eclosion rate of the aphidiusgifuensis is low, successful egg laying amount is small and industrialized production is not easily realized when the aphidiusgifuensis is artificially bred. Collecting and breeding technology of the host aphids and species of the aphidiusgifuensis is provided in the artificially breeding process, the technology of host plant cultivation, inoculation breeding of the aphids and breeding of the mummified aphids and the adult bees of the aphidiusgifuensis is provided, the method has the advantages of short period, high efficiency, high breeding speed and low cost, the mummified aphids of the aphidiusgifuensis can be largely bred, the mummified aphids and the adult bees of the aphidiusgifuensis are produced on a large scale, and the myzuspersicae, English grain aphids and cotton aphids can be effectively prevented in the technical field of biological prevention.
Description
Technical field
The invention belongs to the biological prevention field of pest, be specifically related in the process of biological control cigarette aphid insect by the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee.
Background technology
Aphidius gifuensis is a kind of parasitic wasp, in agricultural production, often utilizes aphidius gifuensis to carry out the various aphids of biological control.In utilizing the anti-process eliminated aphis of aphidius gifuensis, need to be at first technological means by artificial propagation obtain the stiff aphid of a large amount of aphidius gifuensis or become honeybee, then will breed the stiff aphid of the aphidius gifuensis obtained or become honeybee to be discharged into the serious area of aphid damage, aphidius gifuensis originates in ovum in the aphid body, the ovum of aphidius gifuensis is grown by the nutriment that consumes aphid after colonizing in the aphid body, finally eliminates aphid.When artificial propagation aphidius gifuensis deadlock aphid or one-tenth honeybee, what people applied usually at present is the host aphid of red type cigarette aphid as aphidius gifuensis, and the individuality of this host aphid is smaller, body wall is thick, uses flue-cured tobacco cultivars as host plant.So the stiff aphid of the aphidius gifuensis that obtains of breeding is also smaller, the aphidius gifuensis eclosion rate is low, and the life-span is short, collects more difficultly, and collection efficiency is very low; Simultaneously, because host plant cigarette strain growth cycle is long, cultivate difficulty winter, and energy resource consumption is higher, and the quality of the stiff aphid of the aphidius gifuensis obtained is also poor, is unfavorable for the aphidius gifuensis large-scale breeding.
Summary of the invention
In agricultural production, people utilize aphidius gifuensis control aphid damage, when the artificial propagation aphidius gifuensis, because the host aphid individuality of the aphidius gifuensis of available technology adopting is little, body wall is thick, aphidius gifuensis is laid eggs and is not easy on the host aphid, host plant cigarette strain growth cycle is long, cultivate difficulty winter, the aphidius gifuensis individuality that breeding obtains is little, eclosion rate is low, life-span is short, the stiff aphid of aphidius gifuensis firm pasting on host plant, collect difficulty, efficiency is low, be difficult for realizing aphidius gifuensis batch production large-scale production, for the above-mentioned problems in the prior art, the invention provides the stiff aphid of the yellowish green colour pattern cigarette of a kind of use aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, the yellowish green colour pattern cigarette aphid that the method utilization individuality is larger bred with the stiff aphid of cultivating aphidius gifuensis with become honeybee, cigarette aphid for the biological control agricultural production, English grain aphid and cotten aphid, concrete implementation step is as follows:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 20-30 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 3-4 sheet true leaf, raise 5-7 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in the host plant of 30-60% carry out the old and new and replace, the aphid kind of breeding on a large scale yellowish green colour pattern cigarette aphid the anniversary;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:50-100, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, sneak case according to aphidius gifuensis on yellowish green colour pattern cigarette aphid and the growing state of host plant, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control at 20-50%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 4-8 strain, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, host plant is cultivated 20-30 days in breeding chamber;
Breed the disease-resistant leafy host plant kind of the close aphid of planting in chamber and be green vegetables, radish or blue and white in a kind of;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 1-2cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is every strain host plant 30-50 head, placement is many facing up of aphid during with the small pieces of aphid kind, small pieces with the aphid kind are placed 1-2 days the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 5-7 days the carbendazim 800-1000 times liquid of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, yellowish green colour pattern cigarette aphid continues to breed 10-15 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:40-80 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, aphidius gifuensis is bred 7-14 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) the stiff aphid of aphidius gifuensis or one-tenth honeybee are collected
After step (5) finishes, to obtain the stiff aphid blade of aphidius gifuensis collects in batches, the stiff aphid blade collected is carried out to the stiff aphid separating treatment of cigarette aphid and aphidius gifuensis, and the stiff aphid of the aphidius gifuensis then separation obtained carries out stored refrigerated or is applied directly to the aphid area of causing harm;
Perhaps, after step (5) finishes, the stiff aphid of the aphidius gifuensis obtained continues to cultivate 3-5 day, and the stiff aphid of a large amount of aphidius gifuensis obtains aphidius gifuensis after growing and sprouting wings and becomes honeybee, with cocoon honeybee collector, is gathered into after honeybee and is discharged into the aphid area of causing harm.
The present invention adopts green vegetables, radish or the blue and white host plant as aphid of Cruciferae, selects the host aphid of yellowish green colour pattern cigarette aphid as aphidius gifuensis, and this host plant parent aphid is disease-resistant leafy, is conducive to yellowish green colour pattern cigarette aphid growth reproduction on the host plants; Yellowish green colour pattern cigarette aphid individuality is larger, aphidius gifuensis carries out the egg reproduction ratio and is easier in the body of yellowish green colour pattern cigarette aphid, and successful egg laying amount is large, and efficiency is higher, the aphidius gifuensis reproduction speed is fast, the cycle is short, is conducive to the stiff aphid of aphidius gifuensis and the large-scale industrialized production that becomes honeybee; The stiff aphid of the aphidius gifuensis that obtains with become honeybee for preventing and treating cigarette aphid, English grain aphid and cotten aphid, control efficiency is remarkable.
Embodiment
embodiment 1
By the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, concrete implementation step is as follows:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 20 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 3 true leaves, raise 5 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 17 ℃ of temperature, RH40%, illumination every day 8 hours, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in 30% host plant carry out the old and new and replace, the aphid kind of breeding on a large scale yellowish green colour pattern cigarette aphid the anniversary;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:50, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is 17 ℃ of temperature, RH40%, illumination every day 8 hours, sneak case according to aphidius gifuensis on yellowish green colour pattern cigarette aphid and the growing state of host plant, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control 20%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 4 strains, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 17 ℃ of temperature, RH40%, illumination every day 8 hours, host plant is cultivated 20 days in breeding chamber;
Breeding the disease-resistant leafy host plant kind of the close aphid of planting in chamber is green vegetables;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 1cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is 30 of every strain host plants, placement is many facing up of aphid during with the small pieces of aphid kind, small pieces with the aphid kind are placed 1 day the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 5 days 800 times of liquid of carbendazim of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 8 hours, 17 ℃ of temperature, RH40%, yellowish green colour pattern cigarette aphid continues to breed 10 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:40 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 8 hours, 17 ℃ of temperature, RH40%, aphidius gifuensis is bred 7 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) the stiff aphid of aphidius gifuensis is collected
After step (5) finishes, to obtain the stiff aphid blade of aphidius gifuensis collects in batches, the stiff aphid blade collected is carried out to the stiff aphid separating treatment of cigarette aphid and aphidius gifuensis, and the stiff aphid of the aphidius gifuensis then separation obtained carries out stored refrigerated, is applied to the aphid area of causing harm while needing.
embodiment 2
By the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, concrete implementation step is as follows:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 30 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 4 true leaves, raise 7 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 28 ℃ of temperature, RH70%, illumination every day 12 hours, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in 60% host plant carry out the old and new and replace, the aphid kind of breeding on a large scale yellowish green colour pattern cigarette aphid the anniversary;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:100, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is 28 ℃ of temperature, RH70%, illumination every day 12 hours, sneak case according to aphidius gifuensis on yellowish green colour pattern cigarette aphid and the growing state of host plant, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control 50%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 8 strains, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 28 ℃ of temperature, RH70%, illumination every day 12 hours, host plant is cultivated 30 days in breeding chamber;
It is blue and white breeding the disease-resistant leafy host plant kind of the close aphid of planting in chamber;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 2cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is 50 of every strain host plants, placement is many facing up of aphid during with the small pieces of aphid kind, small pieces with the aphid kind are placed 2 days the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 7 days 1000 times of liquid of carbendazim of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 12 hours, 28 ℃ of temperature, RH70%, yellowish green colour pattern cigarette aphid continues to breed 15 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:80 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 12 hours, 28 ℃ of temperature, RH70%, aphidius gifuensis is bred 14 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) aphidius gifuensis becomes honeybee to collect
After step (5) finishes, the stiff aphid of the aphidius gifuensis obtained continues to cultivate 5, and the stiff aphid of a large amount of aphidius gifuensis obtains aphidius gifuensis after growing and sprouting wings and becomes honeybee, with cocoon honeybee collector, is gathered into after honeybee and is discharged into the aphid area of causing harm.
Claims (1)
1. by the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, it is characterized in that:
With individuality, larger yellowish green colour pattern cigarette aphid is as the host aphid of aphidius gifuensis; With the disease-resistant leafy Cruciferae green vegetables of close aphid, radish or the blue and white host plant as yellowish green colour pattern cigarette aphid; The stiff aphid of extensive artificial propagation aphidius gifuensis with become honeybee, for cigarette aphid, English grain aphid and the cotten aphid of biological control agricultural production; The method comprises the following steps:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 20-30 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 3-4 sheet true leaf, raise 5-7 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in the host plant of 30-60% carry out the old and new and replace;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:50-100, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control at 20-50%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 4-8 strain, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, host plant is cultivated 20-30 days in breeding chamber;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 1-2cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is every strain host plant 30-50 head, placement is during with the small pieces of aphid kind, what aphid was many faces up, small pieces with the aphid kind are placed 1-2 days the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 5-7 days the carbendazim 800-1000 times liquid of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, yellowish green colour pattern cigarette aphid continues to breed 10-15 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:40-80 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, aphidius gifuensis is bred 7-14 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) the stiff aphid of aphidius gifuensis or one-tenth honeybee are collected
After step (5) finishes, to obtain the stiff aphid blade of aphidius gifuensis collects in batches, the stiff aphid blade collected is carried out to the stiff aphid separating treatment of cigarette aphid and aphidius gifuensis, and the stiff aphid of the aphidius gifuensis then separation obtained carries out stored refrigerated or is applied directly to the aphid area of causing harm;
Perhaps, after step (5) finishes, the stiff aphid of the aphidius gifuensis obtained continues to cultivate 3-5 day, and the stiff aphid of a large amount of aphidius gifuensis obtains aphidius gifuensis after growing and sprouting wings and becomes honeybee, with cocoon honeybee collector, is gathered into after honeybee and is discharged into the aphid area of causing harm.
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