CN103478083A - Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae - Google Patents

Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae Download PDF

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CN103478083A
CN103478083A CN201310449593.5A CN201310449593A CN103478083A CN 103478083 A CN103478083 A CN 103478083A CN 201310449593 A CN201310449593 A CN 201310449593A CN 103478083 A CN103478083 A CN 103478083A
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aphid
host plant
aphidius gifuensis
honeybee
breeding
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CN103478083B (en
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任伟
刘虹伶
吴云波
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Yunnan greenery biological and ecological methods to prevent plant disease, pests, and erosion Science and Technology Ltd.
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任伟
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Abstract

Disclosed is a large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae. The yellow-green myzuspersicae with large bodies serve as host aphids of aphidiusgifuensis, and parent aphid disease-resistant leafy cruciferous green vegetables, radishes or sweet blue flowers serve as host plants of the yellow-green myzuspersicae, so that the mummified aphids and the adult bees of the aphidiusgifuensis are artificially bred on a large scale. The method solves the problems that the bodies of the host aphids are small, the mummified aphids are difficultly collected, the eclosion rate of the aphidiusgifuensis is low, successful egg laying amount is small and industrialized production is not easily realized when the aphidiusgifuensis is artificially bred. Collecting and breeding technology of the host aphids and species of the aphidiusgifuensis is provided in the artificially breeding process, the technology of host plant cultivation, inoculation breeding of the aphids and breeding of the mummified aphids and the adult bees of the aphidiusgifuensis is provided, the method has the advantages of short period, high efficiency, high breeding speed and low cost, the mummified aphids of the aphidiusgifuensis can be largely bred, the mummified aphids and the adult bees of the aphidiusgifuensis are produced on a large scale, and the myzuspersicae, English grain aphids and cotton aphids can be effectively prevented in the technical field of biological prevention.

Description

By the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee
Technical field
The invention belongs to the biological prevention field of pest, be specifically related in the process of biological control cigarette aphid insect by the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee.
Background technology
Aphidius gifuensis is a kind of parasitic wasp, in agricultural production, often utilizes aphidius gifuensis to carry out the various aphids of biological control.In utilizing the anti-process eliminated aphis of aphidius gifuensis, need to be at first technological means by artificial propagation obtain the stiff aphid of a large amount of aphidius gifuensis or become honeybee, then will breed the stiff aphid of the aphidius gifuensis obtained or become honeybee to be discharged into the serious area of aphid damage, aphidius gifuensis originates in ovum in the aphid body, the ovum of aphidius gifuensis is grown by the nutriment that consumes aphid after colonizing in the aphid body, finally eliminates aphid.When artificial propagation aphidius gifuensis deadlock aphid or one-tenth honeybee, what people applied usually at present is the host aphid of red type cigarette aphid as aphidius gifuensis, and the individuality of this host aphid is smaller, body wall is thick, uses flue-cured tobacco cultivars as host plant.So the stiff aphid of the aphidius gifuensis that obtains of breeding is also smaller, the aphidius gifuensis eclosion rate is low, and the life-span is short, collects more difficultly, and collection efficiency is very low; Simultaneously, because host plant cigarette strain growth cycle is long, cultivate difficulty winter, and energy resource consumption is higher, and the quality of the stiff aphid of the aphidius gifuensis obtained is also poor, is unfavorable for the aphidius gifuensis large-scale breeding.
Summary of the invention
In agricultural production, people utilize aphidius gifuensis control aphid damage, when the artificial propagation aphidius gifuensis, because the host aphid individuality of the aphidius gifuensis of available technology adopting is little, body wall is thick, aphidius gifuensis is laid eggs and is not easy on the host aphid, host plant cigarette strain growth cycle is long, cultivate difficulty winter, the aphidius gifuensis individuality that breeding obtains is little, eclosion rate is low, life-span is short, the stiff aphid of aphidius gifuensis firm pasting on host plant, collect difficulty, efficiency is low, be difficult for realizing aphidius gifuensis batch production large-scale production, for the above-mentioned problems in the prior art, the invention provides the stiff aphid of the yellowish green colour pattern cigarette of a kind of use aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, the yellowish green colour pattern cigarette aphid that the method utilization individuality is larger bred with the stiff aphid of cultivating aphidius gifuensis with become honeybee, cigarette aphid for the biological control agricultural production, English grain aphid and cotten aphid, concrete implementation step is as follows:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 20-30 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 3-4 sheet true leaf, raise 5-7 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in the host plant of 30-60% carry out the old and new and replace, the aphid kind of breeding on a large scale yellowish green colour pattern cigarette aphid the anniversary;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:50-100, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, sneak case according to aphidius gifuensis on yellowish green colour pattern cigarette aphid and the growing state of host plant, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control at 20-50%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 4-8 strain, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, host plant is cultivated 20-30 days in breeding chamber;
Breed the disease-resistant leafy host plant kind of the close aphid of planting in chamber and be green vegetables, radish or blue and white in a kind of;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 1-2cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is every strain host plant 30-50 head, placement is many facing up of aphid during with the small pieces of aphid kind, small pieces with the aphid kind are placed 1-2 days the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 5-7 days the carbendazim 800-1000 times liquid of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, yellowish green colour pattern cigarette aphid continues to breed 10-15 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:40-80 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, aphidius gifuensis is bred 7-14 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) the stiff aphid of aphidius gifuensis or one-tenth honeybee are collected
After step (5) finishes, to obtain the stiff aphid blade of aphidius gifuensis collects in batches, the stiff aphid blade collected is carried out to the stiff aphid separating treatment of cigarette aphid and aphidius gifuensis, and the stiff aphid of the aphidius gifuensis then separation obtained carries out stored refrigerated or is applied directly to the aphid area of causing harm;
Perhaps, after step (5) finishes, the stiff aphid of the aphidius gifuensis obtained continues to cultivate 3-5 day, and the stiff aphid of a large amount of aphidius gifuensis obtains aphidius gifuensis after growing and sprouting wings and becomes honeybee, with cocoon honeybee collector, is gathered into after honeybee and is discharged into the aphid area of causing harm.
The present invention adopts green vegetables, radish or the blue and white host plant as aphid of Cruciferae, selects the host aphid of yellowish green colour pattern cigarette aphid as aphidius gifuensis, and this host plant parent aphid is disease-resistant leafy, is conducive to yellowish green colour pattern cigarette aphid growth reproduction on the host plants; Yellowish green colour pattern cigarette aphid individuality is larger, aphidius gifuensis carries out the egg reproduction ratio and is easier in the body of yellowish green colour pattern cigarette aphid, and successful egg laying amount is large, and efficiency is higher, the aphidius gifuensis reproduction speed is fast, the cycle is short, is conducive to the stiff aphid of aphidius gifuensis and the large-scale industrialized production that becomes honeybee; The stiff aphid of the aphidius gifuensis that obtains with become honeybee for preventing and treating cigarette aphid, English grain aphid and cotten aphid, control efficiency is remarkable.
Embodiment
embodiment 1
By the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, concrete implementation step is as follows:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 20 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 3 true leaves, raise 5 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 17 ℃ of temperature, RH40%, illumination every day 8 hours, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in 30% host plant carry out the old and new and replace, the aphid kind of breeding on a large scale yellowish green colour pattern cigarette aphid the anniversary;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:50, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is 17 ℃ of temperature, RH40%, illumination every day 8 hours, sneak case according to aphidius gifuensis on yellowish green colour pattern cigarette aphid and the growing state of host plant, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control 20%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 4 strains, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 17 ℃ of temperature, RH40%, illumination every day 8 hours, host plant is cultivated 20 days in breeding chamber;
Breeding the disease-resistant leafy host plant kind of the close aphid of planting in chamber is green vegetables;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 1cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is 30 of every strain host plants, placement is many facing up of aphid during with the small pieces of aphid kind, small pieces with the aphid kind are placed 1 day the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 5 days 800 times of liquid of carbendazim of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 8 hours, 17 ℃ of temperature, RH40%, yellowish green colour pattern cigarette aphid continues to breed 10 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:40 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 8 hours, 17 ℃ of temperature, RH40%, aphidius gifuensis is bred 7 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) the stiff aphid of aphidius gifuensis is collected
After step (5) finishes, to obtain the stiff aphid blade of aphidius gifuensis collects in batches, the stiff aphid blade collected is carried out to the stiff aphid separating treatment of cigarette aphid and aphidius gifuensis, and the stiff aphid of the aphidius gifuensis then separation obtained carries out stored refrigerated, is applied to the aphid area of causing harm while needing.
embodiment 2
By the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, concrete implementation step is as follows:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 30 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 4 true leaves, raise 7 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 28 ℃ of temperature, RH70%, illumination every day 12 hours, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in 60% host plant carry out the old and new and replace, the aphid kind of breeding on a large scale yellowish green colour pattern cigarette aphid the anniversary;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:100, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is 28 ℃ of temperature, RH70%, illumination every day 12 hours, sneak case according to aphidius gifuensis on yellowish green colour pattern cigarette aphid and the growing state of host plant, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control 50%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 8 strains, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at 28 ℃ of temperature, RH70%, illumination every day 12 hours, host plant is cultivated 30 days in breeding chamber;
It is blue and white breeding the disease-resistant leafy host plant kind of the close aphid of planting in chamber;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 2cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is 50 of every strain host plants, placement is many facing up of aphid during with the small pieces of aphid kind, small pieces with the aphid kind are placed 2 days the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 7 days 1000 times of liquid of carbendazim of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 12 hours, 28 ℃ of temperature, RH70%, yellowish green colour pattern cigarette aphid continues to breed 15 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:80 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 12 hours, 28 ℃ of temperature, RH70%, aphidius gifuensis is bred 14 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) aphidius gifuensis becomes honeybee to collect
After step (5) finishes, the stiff aphid of the aphidius gifuensis obtained continues to cultivate 5, and the stiff aphid of a large amount of aphidius gifuensis obtains aphidius gifuensis after growing and sprouting wings and becomes honeybee, with cocoon honeybee collector, is gathered into after honeybee and is discharged into the aphid area of causing harm.

Claims (1)

1. by the stiff aphid of yellowish green colour pattern cigarette aphid breeding aphidius gifuensis and the scale propagation method that becomes honeybee, it is characterized in that:
With individuality, larger yellowish green colour pattern cigarette aphid is as the host aphid of aphidius gifuensis; With the disease-resistant leafy Cruciferae green vegetables of close aphid, radish or the blue and white host plant as yellowish green colour pattern cigarette aphid; The stiff aphid of extensive artificial propagation aphidius gifuensis with become honeybee, for cigarette aphid, English grain aphid and the cotten aphid of biological control agricultural production; The method comprises the following steps:
(1) gather yellowish green colour pattern cigarette aphid, detoxification is bred the aphid kind after purifying
The disease-resistant Cruciferae host plant of aphid kind indoor seeding parent aphid, the Cruciferae host plant is cultivated 20-30 days after the indoor seeding of aphid kind;
Gather the yellowish green colour pattern cigarette aphid large without sick body from the field crucifer, the yellowish green colour pattern cigarette aphid that field is collected is raised length to be had on the radish dish seedling of 3-4 sheet true leaf, raise 5-7 days, make the cigarette aphid detoxification of raising, after detoxification finishes, remove by the aphidius gifuensis parasitism, in spite of illness and cigarette aphids all the other colour patterns, with the yellowish green colour pattern cigarette aphid of purifying;
Yellowish green colour pattern cigarette aphid after purifying is raised on the Cruciferae host plant in aphid kind chamber, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, the aphid amount in aphid kind chamber on every strain host plant reaches 1000 aphid kinds as large-scale industrialized Reproduction when above;
Every 15 days to aphid kind chamber in the host plant of 30-60% carry out the old and new and replace;
(2) gather aphidius gifuensis, breed the honeybee kind after purification
Gather aphidius gifuensis from field, reject aphidius gifuensis parasitic wasp in addition, obtain the pure aphidius gifuensis of kind;
A part of aphid kind that step (1) is obtained moves into honeybee kind chamber together with host plant, by the kind obtained, pure aphidius gifuensis is discharged in honeybee kind chamber, the burst size of controlling aphidius gifuensis is that the honeybee aphid is compared 1:50-100, the host plant liquid manure that guarantees the aphid kind is suitable, controlling environmental condition in honeybee kind chamber is temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, the host plant with the aphid kind obtained by step (1) every 15 days and the host plant in honeybee kind chamber carry out the old and new and change, upgrade proportion control at 20-50%, breed in enormous quantities the honeybee kind that obtains aphidius gifuensis,
(3) prepare to breed chamber, cultivate host plant
Adopt insect propagating and breeding canopy as breeding chamber, the chamber of breeding selects to be based upon that physical features is higher, draining is convenient, it is local preferably to ventilate;
The disease-resistant leafy host plant of the close aphid of plantation in breeding chamber, the planting density of host plant is controlled at every square metre of 4-8 strain, host plant is carried out to suitable water and fertilizer management, breed indoor environmental condition and be controlled at temperature 17-28 ℃, RH40-70%, illumination every day 8-12 hour, host plant is cultivated 20-30 days in breeding chamber;
(4) yellowish green colour pattern cigarette aphid aphid inoculates and breeds
All the other picking blades of the host plant with the aphid kind that step (1) is obtained get off, the blade of the host plant with the aphid kind that picks is torn into to the small pieces of wide 1-2cm, obtain the small pieces with the aphid kind, then will be placed on the small pieces of aphid kind and breed after step in chamber (3) finishes the face of blade that is positioned at middle and lower part on host plant and carry out yellowish green colour pattern cigarette aphid aphid inoculation, controlling the aphid amount discharged with the small pieces with the aphid kind is every strain host plant 30-50 head, placement is during with the small pieces of aphid kind, what aphid was many faces up, small pieces with the aphid kind are placed 1-2 days the face of blade of host plant, then remove aphid kind small pieces, breed and host plant was sprayed in 5-7 days the carbendazim 800-1000 times liquid of one time 25% after removing aphid kind small pieces, prevention and control host plant and cigarette aphid generation disease,
Keep breeding in chamber the host plant water and fertilizer management suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, yellowish green colour pattern cigarette aphid continues to breed 10-15 days in breeding chamber, aphid amount on every strain host plant reaches more than 2000, and wherein the yellowish green colour pattern cigarette aphid more than 50% reaches 2-3 age;
(5) aphidius gifuensis connects honeybee and the stiff aphid cultivation of aphidius gifuensis
After a large amount of emergence of honeybee kind that obtains aphidius gifuensis until step (2), collect the one-tenth honeybee of aphidius gifuensis from honeybee kind chamber with cocoon honeybee collector, breeding in chamber after the one-tenth honeybee that collection is obtained is discharged into step (4) and finishes than 1:40-80 according to the honeybee aphid, keep breeding in chamber host plant liquid manure suitable, illumination every day 8-12 hour, temperature 17-28 ℃, RH40-70%, aphidius gifuensis is bred 7-14 days in breeding chamber, yellowish green colour pattern cigarette aphid on the host plant blade has successfully been laid eggs by aphidius gifuensis more than 80% and is parasitic, obtain the stiff aphid of aphidius gifuensis, the stiff aphid of aphidius gifuensis more than 50% is wherein arranged in pupa time,
(6) the stiff aphid of aphidius gifuensis or one-tenth honeybee are collected
After step (5) finishes, to obtain the stiff aphid blade of aphidius gifuensis collects in batches, the stiff aphid blade collected is carried out to the stiff aphid separating treatment of cigarette aphid and aphidius gifuensis, and the stiff aphid of the aphidius gifuensis then separation obtained carries out stored refrigerated or is applied directly to the aphid area of causing harm;
Perhaps, after step (5) finishes, the stiff aphid of the aphidius gifuensis obtained continues to cultivate 3-5 day, and the stiff aphid of a large amount of aphidius gifuensis obtains aphidius gifuensis after growing and sprouting wings and becomes honeybee, with cocoon honeybee collector, is gathered into after honeybee and is discharged into the aphid area of causing harm.
CN201310449593.5A 2013-09-29 2013-09-29 Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae Active CN103478083B (en)

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CN104756955A (en) * 2015-03-11 2015-07-08 中国烟草总公司四川省公司 Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method
CN104823923A (en) * 2015-03-04 2015-08-12 云南绿叶生防科技有限公司 Breeding method for preventing aphidius gifuensis species from degrading
CN105104311A (en) * 2015-09-25 2015-12-02 黔南州烟草公司瓮安县分公司 Method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides
CN105165733A (en) * 2015-09-25 2015-12-23 黔南州烟草公司瓮安县分公司 Overwintering breeding method for aphidius gifuensis population
CN105165737A (en) * 2015-10-13 2015-12-23 中国农业科学院棉花研究所 Artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica
CN105165313A (en) * 2015-07-24 2015-12-23 中国烟草总公司四川省公司 Method for breeding aphidius gifuensis within short cycle
CN105475232A (en) * 2014-10-13 2016-04-13 郑州正合化学制品有限公司 Method for large-scale breeding of aphidiidae and method for control of aphids
CN106305227A (en) * 2016-08-23 2017-01-11 高啟荣 Method for preventing and controlling aphids by breeding syrphidae
CN106614393A (en) * 2016-11-16 2017-05-10 西北农林科技大学 Carrier plant system and construction method thereof
CN106614392A (en) * 2016-09-29 2017-05-10 中国烟草总公司陕西省公司 Localization method for breeding and releasing of aphidius gifuensis ashmead in Shaanxi area
CN106689066A (en) * 2016-12-08 2017-05-24 辽宁省烟草公司朝阳市公司 Method for quickly breeding aphidius gifuensis
CN107318789A (en) * 2017-08-28 2017-11-07 云南省烟草公司昆明市公司 A kind of field rapid propagation method of aphidius gifuensis
CN107624720A (en) * 2017-11-06 2018-01-26 山东省农业科学院植物保护研究所 A kind of aphid method for breeding and its application suitable for aphidius gifuensis breeding
CN108207822A (en) * 2018-03-12 2018-06-29 内蒙古农业大学 A kind of artificial a large amount of fast breeding technical methods of brown band aphid chalcid fly
CN109122598A (en) * 2018-10-31 2019-01-04 云南省烟草公司文山州公司 A kind of method of Turnip Sprouts breeding cigarette aphid
CN109197782A (en) * 2018-08-08 2019-01-15 云南省烟草公司玉溪市公司 A kind of the selecting quantitative separating and storage method for releasing of deadlock aphid
CN110012867A (en) * 2019-04-29 2019-07-16 中国农业科学院棉花研究所 A kind of cotten aphid sexupara, male aphid, the female indoor highly effective abductive approach of property
CN111406713A (en) * 2019-01-08 2020-07-14 中国科学院动物研究所 Method for breeding parasitic wasps on large scale
CN113040095A (en) * 2019-12-27 2021-06-29 云南格瑞生物科技有限公司 Method for separating and purifying stiff aphids of aphidiidae
CN114938794A (en) * 2022-05-06 2022-08-26 云南省农业科学院经济作物研究所 Method for preventing and treating safflower aphids by aphidiidae

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CN105475232A (en) * 2014-10-13 2016-04-13 郑州正合化学制品有限公司 Method for large-scale breeding of aphidiidae and method for control of aphids
CN104472284A (en) * 2014-12-10 2015-04-01 黔西南州烟草公司兴义市分公司 Method of controlling aphids by intercropping vegetables among tobacco plants to breed Aphidius gifuensis Ashmead
CN104642267A (en) * 2015-02-09 2015-05-27 云南绿叶生防科技有限公司 Industrial cultivation method for aphidius gifuensis ashmead
CN104642267B (en) * 2015-02-09 2018-05-04 云南绿叶生防科技有限公司 A kind of industrialization breeding method of aphidius gifuensis
CN104823923A (en) * 2015-03-04 2015-08-12 云南绿叶生防科技有限公司 Breeding method for preventing aphidius gifuensis species from degrading
CN104823923B (en) * 2015-03-04 2017-05-31 云南绿叶生防科技有限公司 A kind of mating system for preventing aphidius gifuensis population deterioration
CN104756955A (en) * 2015-03-11 2015-07-08 中国烟草总公司四川省公司 Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method
CN104756955B (en) * 2015-03-11 2017-03-08 中国烟草总公司四川省公司 Aphidius gifuensis are with numerous method together with Encarsia formosa
CN105165313A (en) * 2015-07-24 2015-12-23 中国烟草总公司四川省公司 Method for breeding aphidius gifuensis within short cycle
CN105165313B (en) * 2015-07-24 2018-09-14 中国烟草总公司四川省公司 Aphidius gifuensis short cycle mating system
CN105165733A (en) * 2015-09-25 2015-12-23 黔南州烟草公司瓮安县分公司 Overwintering breeding method for aphidius gifuensis population
CN105104311A (en) * 2015-09-25 2015-12-02 黔南州烟草公司瓮安县分公司 Method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides
CN105165737A (en) * 2015-10-13 2015-12-23 中国农业科学院棉花研究所 Artificial feeding method of cotton aphid parasitoid wasp-Lysiphlebia japonica
CN105165737B (en) * 2015-10-13 2017-10-31 中国农业科学院棉花研究所 A kind of rearing method of cotten aphid parasitic wasp cotton aphid parasite
CN106305227A (en) * 2016-08-23 2017-01-11 高啟荣 Method for preventing and controlling aphids by breeding syrphidae
CN106614392A (en) * 2016-09-29 2017-05-10 中国烟草总公司陕西省公司 Localization method for breeding and releasing of aphidius gifuensis ashmead in Shaanxi area
CN106614393A (en) * 2016-11-16 2017-05-10 西北农林科技大学 Carrier plant system and construction method thereof
CN106689066A (en) * 2016-12-08 2017-05-24 辽宁省烟草公司朝阳市公司 Method for quickly breeding aphidius gifuensis
CN107318789A (en) * 2017-08-28 2017-11-07 云南省烟草公司昆明市公司 A kind of field rapid propagation method of aphidius gifuensis
CN107624720A (en) * 2017-11-06 2018-01-26 山东省农业科学院植物保护研究所 A kind of aphid method for breeding and its application suitable for aphidius gifuensis breeding
CN108207822A (en) * 2018-03-12 2018-06-29 内蒙古农业大学 A kind of artificial a large amount of fast breeding technical methods of brown band aphid chalcid fly
CN108207822B (en) * 2018-03-12 2020-10-02 内蒙古农业大学 Artificial mass rapid breeding technical method for Aphidius fusceolatus
CN109197782A (en) * 2018-08-08 2019-01-15 云南省烟草公司玉溪市公司 A kind of the selecting quantitative separating and storage method for releasing of deadlock aphid
CN109197782B (en) * 2018-08-08 2021-03-02 云南省烟草公司玉溪市公司 Method for collecting, selecting, quantitatively subpackaging, storing and releasing stiff aphids
CN109122598A (en) * 2018-10-31 2019-01-04 云南省烟草公司文山州公司 A kind of method of Turnip Sprouts breeding cigarette aphid
CN111406713A (en) * 2019-01-08 2020-07-14 中国科学院动物研究所 Method for breeding parasitic wasps on large scale
CN111406713B (en) * 2019-01-08 2021-08-24 中国科学院动物研究所 Method for breeding parasitic wasps on large scale
CN110012867A (en) * 2019-04-29 2019-07-16 中国农业科学院棉花研究所 A kind of cotten aphid sexupara, male aphid, the female indoor highly effective abductive approach of property
CN110012867B (en) * 2019-04-29 2021-09-10 中国农业科学院棉花研究所 Cotton aphid sex mother, male aphid and sex female indoor efficient induction method
CN113040095A (en) * 2019-12-27 2021-06-29 云南格瑞生物科技有限公司 Method for separating and purifying stiff aphids of aphidiidae
CN114938794A (en) * 2022-05-06 2022-08-26 云南省农业科学院经济作物研究所 Method for preventing and treating safflower aphids by aphidiidae

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