CN105165313B - Aphidius gifuensis short cycle mating system - Google Patents

Aphidius gifuensis short cycle mating system Download PDF

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Publication number
CN105165313B
CN105165313B CN201510440277.0A CN201510440277A CN105165313B CN 105165313 B CN105165313 B CN 105165313B CN 201510440277 A CN201510440277 A CN 201510440277A CN 105165313 B CN105165313 B CN 105165313B
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plant
aphidius gifuensis
aphid
brassica rapa
breeding
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CN105165313A (en
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李斌
雷强
张炳科
张瑞平
曾庆宾
闫芳芳
刘杨
信俊锋
曾宗梁
杨章明
任伟
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Panzhihua City Company Sichuan Tobacco Co (panzhihua City Tobacco Company)
Yunnan Greenery Biological And Ecological Methods To Prevent Plant Disease Pests And Erosion Science And Technology Ltd
SICHUAN PROVINCE Co OF CHINA NATIONAL TOBACCO Corp
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Panzhihua City Company Sichuan Tobacco Co (panzhihua City Tobacco Company)
Yunnan Greenery Biological And Ecological Methods To Prevent Plant Disease Pests And Erosion Science And Technology Ltd
SICHUAN PROVINCE Co OF CHINA NATIONAL TOBACCO Corp
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

Abstract

Aphidius gifuensis short cycle mating system,Step includes host plant cultivation,Worm kind is bred,Aphidius gifuensis Ashmaed scale is bred,The overall process period is bred at 29 40 days,Often disk Brassica rapa L can educate to obtain cigarette aphid 12000 or more,Aphidius gifuensis parasitic rate 95%,Stiff aphid formation rate 60%,Often disk aphidius gifuensis deadlock aphid can be used to prevent 10 15 mu of farmland of aphid evil,Host plant is made using vegetable variety Brassica rapa L,It is adaptable,Growth and development is fast,It is the shortest host plant kind of cultivation period in current aphidius gifuensis raising technology,Substantially reduce the breeding cycle of aphidius gifuensis,Improve the breeding efficiency of aphidius gifuensis,Present invention application floating seedlings method cultivates host plant,Processing step is simple,Solves breeding high energy consumption,Techniqueflow is cumbersome,Aphidius gifuensis poor activity,Aphidius gifuensis deadlock aphid forms irregular problem,Aphidius gifuensis can be promoted in the widely applied utilization rate of agriculture and forestry,Aphidius gifuensis is set more to produce,The feasibility of popularization and application.

Description

Aphidius gifuensis short cycle mating system
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry pest, and in particular to a kind of aphidius gifuensis short cycle breeding Method.
Background technology
Aphid is one of the primary pest in farm-forestry crop production, in leaf tobacco production the harm of aphid be particularly acute, it is main Tobacco leaf juice is sucked on the tobacco leaf back of the body and tender stem with adult and nymph, cause cigarette strain to grow short and small, nutritional imbalance, influence cigarette The normal growth and development of strain, the easy generation for inducing the diseases such as dark mildew of honeydew of aphid secretion, while aphid are also propagated a variety of Tobacco virus causes the prevalence of tobacco virus;Mainly there are chemical prevention and physical control, chemical prevention to rely on agriculture aphid Medicine eliminates aphid, bio-diversity can be caused to reduce, and destroys ecological environment, and pollution of agricultural products influences food security, and aphid resists Pharmacological property enhances, and control effect declines, and physical control relies primarily on yellow plate prevention and control alatae worm, when alatae worm colonizes on plant When, prevention and control effect that yellow plate has just scattered and disappeared to aphid.
In Agro-ecology development, biological prevention is anti-one of the most effective approach that eliminates aphis, aphidius gifuensis It is the advantage parasite of cigarette aphid, is distributed throughout our country, ovum is originated in feeding juice in aphid body and killed by aphidius gifuensis Dead or control aphid, breeds the offspring of oneself, it has good control effect to cigarette aphid, while to grain aphid, wheat y-bend The aphids such as aphid, bean aphid, cotten aphid also have certain control effect, currently, aphidius gifuensis artificial breeding technology still locates study demonstration In the stage, there are host plant kinds not to enrich, and host's feeding habits are single, cultivates difficult, aphidius gifuensis length breeding cycle, poor activity, It is numerous to be badly in need of a kind of short-period aphidius gifuensis of stability and high efficiency of research for the problems such as aphidius gifuensis deadlock aphid formation regularity is poor, high energy consumption Educate method.
Invention content
The present invention is in the prior art, and there are host plant kinds not to enrich for artificial breeding aphidius gifuensis, host's feeding habits It is single, difficulty is cultivated, aphidius gifuensis length breeding cycle, poor activity, aphidius gifuensis deadlock aphid formation regularity is poor, high energy consumption, breeding The problem of tedious process, host plant are easy to pass disease biography poison in vega, and aphid invades vega risk, it is short to provide a kind of aphidius gifuensis Period mating system, the host plant that this method uses is vegetable variety Brassica rapa L, applies floating seedlings method and cultivates host's plant Object, steps are as follows for specific breeding:
(1)Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis;
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps aqueous solution in nursery pond Depth be 5-10cm, control nursery pond in aqueous solution a concentration of 150-250ppm of total N;
Floating seedlings special seedlings nursing plate 60-500 are got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, high by 5 Centimetre, each seedlings nursing plate is equipped with 80-162 cultivation cave, and seedling medium has been contained in the cultivation cave of seedlings nursing plate;
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first is in 3-5 seedlings nursing plate Sowing is had 3-5 seedlings nursing plate of Brassica rapa L seed to relay and swum on the aqueous solution of nursery pond by middle sowing Brassica rapa L seed, Floating has the nylon guard of covering 60-80 mesh above the nursery pond of seedlings nursing plate, natural lighting condition is kept, remaining after 10 days Second batch sows Brassica rapa L seed in 55-597 seedlings nursing plate, and second batch sowing is had to 55-597 seedlings nursing plate of Brassica rapa L seed It relays and swims on the aqueous solution of nursery pond, the nylon guard of 60-80 mesh is covered above the nursery pond that floating has seedlings nursing plate, protect Hold natural lighting condition;
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5-11 days long to three leaves wholeheartedly, it is long in Brassica rapa L 500-1000 times of liquid of a Bravo is sprayed to plant when to three leaves wholeheartedly;
(2)Worm kind is bred
Worm kind room is got out, Flotage seedling raising pool is equipped in worm kind room, is contained in the Flotage seedling raising pool in worm kind room Good and step(1)Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step(1)In 3-5 seedlings nursing plate of first sowing Brassica rapa L plant when growing to three leaves wholeheartedly, the 3-5 seedlings nursing plate is relayed in the Flotage seedling raising pool of worm kind room;
It acquires cigarette aphid and aphidius gifuensis respectively to field, puts the collected cigarette aphid in field and aphidius gifuensis into worm kind simultaneously Three leaves often discharge cigarette aphid 500-800, aphidius gifuensis 1-5 heads, guarantor wholeheartedly on the plant of Brassica rapa L in room on disk Brassica rapa L plant 15-30 DEG C of worm kind indoor temperature, humidity 30-80% are held, aphidius gifuensis and cigarette aphid breed 5-10 days jointly in worm kind interior, It is bred on the indoor Brassica rapa L plant leaf of worm kind and obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis, on Brassica rapa L plant leaf Cigarette aphid 10%-20% is reached by the ratio of aphidius gifuensis parasitism, it is spare;
(3)Aphidius gifuensis Ashmaed scale is bred
By step(2)There is under the picking of the Brassica rapa L plant leaf of tobacco aphid kind and aphidius gifuensis worm kind breeding in worm kind room Come, manually the breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to tear by the method for sheet It is equably inoculated into step after growth 4-6cm, width 2-3cm(1)On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, Inoculation blade more than aphid is face-up, and tobacco aphid kind 5-10, aphidius gifuensis worm kind 1-2 heads are inoculated on every plant of Brassica rapa L plant, Composite fertilizer's aqueous solution is added in Flotage seedling raising pool in nylon guard, the total N controlled in nylon guard in Flotage seedling raising pool is dense Degree is maintained at 200-400ppm, breeds 20-30 DEG C of temperature, be inoculated with up to after 2 days by a length of 4-6cm, the inoculation leaf that width is 2-3cm Piece is removed totally, and connect worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf after 3 days, is sprayed to Brassica rapa L plant primary 800 times of carbendazols, it is 25 DEG C, humidity 50% to control breeding temperature in nylon guard, after continuing breeding 10 days, every plant of Brassica rapa L The cigarette aphid quantity bred on plant reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains Composite fertilizer's aqueous solution in nylon guard in Flotage seedling raising pool, the 11 of fine day:00-16:It is covered above nylon guard when 00 The sunshade net of 50%-70%, when Brassica rapa L plant is wilted into nursery pond injected clear water, control injected clear water depth 1- Then 2cm, 1 hour time drain the clear water in nursery pond, the matrix in seedlings nursing plate cultivation cave is kept to moisten Brassica rapa L plant not It wilts, drains and continue breeding after composite fertilizer's aqueous solution and reach 3-5 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches 150 or more, aphidius gifuensis reaches 95% or more to the parasitic rate of cigarette aphid, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more, It removes on Brassica rapa L plant not by the cigarette aphid of aphidius gifuensis parasitism, obtains numerous plant for having aphidius gifuensis deadlock aphid, it will be on plant Obtained aphidius gifuensis deadlock aphid is bred to carry out storage or numerous plant for having aphidius gifuensis deadlock aphid is directly used for field control aphid Evil, often disk is numerous has the plant of aphidius gifuensis deadlock aphid that can prevent farmland 10-15 mus of aphid evil;
The host plant that the present invention uses is vegetable variety Brassica rapa L, further enriches host plant kind;Brassica rapa L It is adaptable, it can be cultivated in most area Zhou Nianjun, solve the host plant of previous kind by natural conditions constraint, cultivation Difficult problem;Brassica rapa L growth and development is fast, sows 3 days and emerges, and cultivates the host's plant that can be used as breeding aphidius gifuensis for 8-14 days Object is the shortest host plant kind of cultivation period in current aphidius gifuensis raising technology, substantially reduces the numerous of aphidius gifuensis Educate the period;Brassica rapa L is the host of cigarette aphid eating, and cigarette aphid growth reproduction speed on Brassica rapa L plant is fast, detoxification is quick, neat Spend, activity is strong, individual is healthy and strong, Brassica rapa L adapts to the nutriment needed for the various cigarette aphid breeding such as tobacco type, wild cabbage, various Cigarette aphid can be good at growth and development on Brassica rapa L, and excellent host can be provided for aphidius gifuensis, can prevent breeding and answer Risk;
The present invention is proposed cultivates host plant Brassica rapa L using float seedling method, provides specific in cultivating process , the technical parameter of strong operability, to breeding the illumination in each link, liquid manure technology requires to have carried out regulation and standardization control System, makes aphidius gifuensis realize the large-scale production and application of batch production, commercialization, and aphidius gifuensis can be made to make in tobacco, vegetables Popularization and application obtain remarkable effect in the productions such as object, while reducing aphidius gifuensis production cost, improve aphidius gifuensis in agriculture The widely applied utilization rate of forestry makes aphidius gifuensis have more the feasibility for producing, promoting and applying, and cultivates host using the present invention and plants Object can not only ensure that the nutritional condition of host plant is excellent, and saving of work and time, solve breeding aphidius gifuensis high energy consumption, skill Art flow is cumbersome, aphidius gifuensis poor activity, aphidius gifuensis deadlock aphid form irregular problem;
The technology of the present invention covers nylon guard above nursery pond, and at low cost, flexibility is strong, can prevent remaining insect Feeding to Brassica rapa L, while also solving the problems, such as cigarette aphid in reproductive process, aphidius gifuensis escape, it ensure that the safety of technology Property, feasibility;Carbendazol in time is sprayed to host plant in reproductive process, ensure that host plants in aphidius gifuensis breeding Object and the anti-characteristic of disease of cigarette aphid, aphidius gifuensis, can improve the safety in aphidius gifuensis reproductive process and stability, ensure that simultaneously Carrying property, high efficiency of the host plant to aphidius gifuensis host cigarette aphid;Present invention process step is simple, simplifies previous breeding skill The technical step bred respectively to cigarette aphid, aphidius gifuensis is needed in art, reduces the energy consumption in reproductive process, improves cigarette aphid cocoon The breeding efficiency of bee.
Specific implementation mode
Embodiment 1:A kind of aphidius gifuensis short cycle mating system, the host plant that this method uses is vegetable variety Shanghai Blueness applies floating seedlings method and cultivates host plant, and steps are as follows for specific breeding:
(1)Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis;
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps aqueous solution in nursery pond Depth be 5cm, control nursery pond in aqueous solution a concentration of 150ppm of total N;
The special seedlings nursing plate of floating seedlings 60 is got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, 5 centimetres high, Each seedlings nursing plate is equipped with 162 cultivation caves, and seedling medium has been contained in the cultivation cave of seedlings nursing plate;
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first is in 3 seedlings nursing plates Brassica rapa L seed is sowed, there are 3 seedlings nursing plates of Brassica rapa L seed to relay sowing and swims on the aqueous solution of nursery pond, floating The nylon guard for having 60 mesh of covering above the nursery pond of seedlings nursing plate, keeps natural lighting condition, is educated at remaining 57 after 10 days Second batch sows Brassica rapa L seed in seed plate, has 57 seedlings nursing plates of Brassica rapa L seed to relay second batch sowing and swims in nursery On the aqueous solution in pond, the nylon guard of 60 mesh is covered above the nursery pond that floating has seedlings nursing plate, keeps natural lighting condition;
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5 days long to three leaves wholeheartedly, Brassica rapa L grow to Three leaves wholeheartedly when to plant spray 500 times of liquid of a Bravo;
(2)Worm kind is bred
Worm kind room is got out, Flotage seedling raising pool is equipped in worm kind room, is contained in the Flotage seedling raising pool in worm kind room Good and step(1)Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step(1)In 3 seedlings nursing plates of first sowing When Brassica rapa L plant grows to three leaves wholeheartedly, 3 seedlings nursing plates are relayed in the Flotage seedling raising pool of worm kind room;
It acquires cigarette aphid and aphidius gifuensis respectively to field, puts the collected cigarette aphid in field and aphidius gifuensis into worm kind simultaneously Three leaves often discharge cigarette aphid 500, aphidius gifuensis 2 wholeheartedly on the plant of Brassica rapa L in room on disk Brassica rapa L plant, keep worm kind 15 DEG C of indoor temperature, humidity 30%, aphidius gifuensis and cigarette aphid were in the indoor common breeding of worm kind 10 days, in the indoor Shanghai of worm kind Breeding obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis on green plant leaf, and the cigarette aphid on Brassica rapa L plant leaf is by cigarette aphid cocoon The ratio of bee parasitism reaches 10%-20%, spare;
(3)Aphidius gifuensis Ashmaed scale is bred
By step(2)There is under the picking of the Brassica rapa L plant leaf of tobacco aphid kind and aphidius gifuensis worm kind breeding in worm kind room Come, manually the breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to tear by the method for sheet It is equably inoculated into step after growth 4cm, width 2cm(1)On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, aphid More inoculation blades is face-up, tobacco aphid kind 10, aphidius gifuensis worm kind 2 is inoculated on every plant of Brassica rapa L plant, in nylon wire Composite fertilizer's aqueous solution is added in Flotage seedling raising pool in cover, controls total N concentration in nylon guard in Flotage seedling raising pool and is maintained at 200ppm breeds 20 DEG C of temperature, is inoculated with clean up to removing a length of 4cm, the inoculation blade that width is 2cm after 2 days, is connect after 3 days Worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf, sprays 800 times of carbendazols to Brassica rapa L plant, controls Buddhist nun It is 25 DEG C, humidity 50% that temperature is bred in imperial guard, is continued after breeding 10 days, the cigarette aphid bred on every plant of Brassica rapa L plant Quantity reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains Flotage seedling raising pool in nylon guard Interior composite fertilizer's aqueous solution, the 11 of fine day:00-16:When 00 above nylon guard covering 50% sunshade net, Brassica rapa L plant Into nursery pond, injected clear water, control injected clear water depth 1cm, are then drained in nursery pond 1 hour time when strain is wilted Clear water, keep the matrix moistening Brassica rapa L plant in seedlings nursing plate cultivation cave not wilt, drain continue after composite fertilizer's aqueous solution it is numerous It educates up to 5 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches 150 or more, parasitism of the aphidius gifuensis to cigarette aphid Rate reaches 95% or more, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more, removes on Brassica rapa L plant not by aphidius gifuensis Parasitic cigarette aphid, obtains numerous plant for having aphidius gifuensis deadlock aphid, the aphidius gifuensis deadlock aphid bred on plant is stored Or directly numerous plant for having an aphidius gifuensis deadlock aphid is done harm to for field control aphid, the every numerous plant energy for having aphidius gifuensis deadlock aphid of disk Enough prevention aphids do harm to 15 mu of farmland.
Embodiment 2:A kind of aphidius gifuensis short cycle mating system, the host plant that this method uses is vegetable variety Shanghai Blueness applies floating seedlings method and cultivates host plant, and steps are as follows for specific breeding:
(1)Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis;
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps aqueous solution in nursery pond Depth be 10cm, control nursery pond in aqueous solution a concentration of 250ppm of total N;
The special seedlings nursing plate of floating seedlings 500 is got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, 5 lis high Rice, each seedlings nursing plate are equipped with 80 cultivation caves, seedling medium have been contained in the cultivation cave of seedlings nursing plate;
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first is in 5 seedlings nursing plates Brassica rapa L seed is sowed, there are 5 seedlings nursing plates of Brassica rapa L seed to relay sowing and swims on the aqueous solution of nursery pond, floating The nylon guard for having 80 mesh of covering above the nursery pond of seedlings nursing plate, keeps natural lighting condition, is educated at remaining 595 after 10 days Second batch sows Brassica rapa L seed in seed plate, has 595 seedlings nursing plates of Brassica rapa L seed to relay to swim in and educate second batch sowing On the aqueous solution of Miao Chi, the nylon guard of 80 mesh is covered above the nursery pond that floating has seedlings nursing plate, keeps natural lighting condition;
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5 days long to three leaves wholeheartedly, Brassica rapa L grow to Three leaves wholeheartedly when to plant spray 1000 times of liquid of a Bravo;
(2)Worm kind is bred
Worm kind room is got out, Flotage seedling raising pool is equipped in worm kind room, is contained in the Flotage seedling raising pool in worm kind room Good and step(1)Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step(1)In 5 seedlings nursing plates of first sowing When Brassica rapa L plant grows to three leaves wholeheartedly, 5 seedlings nursing plates are relayed in the Flotage seedling raising pool of worm kind room;
It acquires cigarette aphid and aphidius gifuensis respectively to field, puts the collected cigarette aphid in field and aphidius gifuensis into worm kind simultaneously Three leaves often discharge cigarette aphid 800, aphidius gifuensis 5 wholeheartedly on the plant of Brassica rapa L in room on disk Brassica rapa L plant, keep worm kind 30 DEG C of indoor temperature, humidity 80%, aphidius gifuensis and cigarette aphid were in the indoor common breeding of worm kind 5 days, in the indoor Brassica rapa L of worm kind Breeding obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis on plant leaf, and the cigarette aphid on Brassica rapa L plant leaf is by aphidius gifuensis Parasitic ratio reaches 10%-20%, spare;
(3)Aphidius gifuensis Ashmaed scale is bred
By step(2)There is under the picking of the Brassica rapa L plant leaf of tobacco aphid kind and aphidius gifuensis worm kind breeding in worm kind room Come, manually the breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to tear by the method for sheet It is equably inoculated into step after growth 6cm, width 3cm(1)On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, aphid More inoculation blades is face-up, tobacco aphid kind 5, aphidius gifuensis worm kind 1 is inoculated on every plant of Brassica rapa L plant, in nylon guard In Flotage seedling raising pool in composite fertilizer aqueous solution is added, control total N concentration in nylon guard in Flotage seedling raising pool and be maintained at 200-400ppm breeds 30 DEG C of temperature, be inoculated with it is clean up to removing a length of 6cm, the inoculation blade that width is 3cm after 2 days, after 3 days Connect worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf, sprays 800 times of carbendazols, control to Brassica rapa L plant It is 25 DEG C, humidity 50% that temperature is bred in nylon guard processed, after continuing breeding 10 days, on every plant of Brassica rapa L plant breeding obtain Cigarette aphid quantity reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains floating in nylon guard and educates Composite fertilizer's aqueous solution in Miao Chi, the 11 of fine day:00-16:The sunshade net for covering 50%-70% when 00 above nylon guard, Into nursery pond, injected clear water, control injected clear water depth 2cm, are then drained 1 hour time when Brassica rapa L plant is wilted Clear water in nursery pond keeps the matrix moistening Brassica rapa L plant in seedlings nursing plate cultivation cave not wilt, drains composite fertilizer's aqueous solution After continue breeding up to 3 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches 150 or more, and aphidius gifuensis is to cigarette The parasitic rate of aphid reaches 95% or more, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more, remove on Brassica rapa L plant not by The cigarette aphid of aphidius gifuensis parasitism obtains numerous plant for having aphidius gifuensis deadlock aphid, the aphidius gifuensis deadlock aphid that will be bred on plant It carries out storage or directly does harm to numerous plant for having aphidius gifuensis deadlock aphid for field control aphid, often disk is numerous aphidius gifuensis deadlock aphid Plant can prevent aphid evil 10 mu of farmland.

Claims (1)

1. aphidius gifuensis short cycle mating system, it is characterised in that:The host plant that this method uses is vegetable variety Brassica rapa L, It applies floating seedlings method and cultivates host plant, steps are as follows for specific breeding:
(1)Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis;
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps the depth of aqueous solution in nursery pond Degree is 5-10cm, and the total nitrogen concentration for controlling aqueous solution in nursery pond is 150-250ppm;
Floating seedlings seedlings nursing plate 60-500 is got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, 5 centimetres high, often A seedlings nursing plate is equipped with 80-162 cultivation cave, and seedling medium has been contained in the cultivation cave of seedlings nursing plate;
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first broadcasts in 3-5 seedlings nursing plate Sowing is had 3-5 seedlings nursing plate of Brassica rapa L seed to relay and swims on the aqueous solution of nursery pond, floating by kind Brassica rapa L seed The nylon guard for having covering 60-80 mesh above the nursery pond of seedlings nursing plate, keeps natural lighting condition, in remaining 55- after 10 days Second batch sows Brassica rapa L seed in 597 seedlings nursing plates, has 55-597 seedlings nursing plate of Brassica rapa L seed to turn second batch sowing Blow up floats on the aqueous solution of nursery pond, and the nylon guard of 60-80 mesh is covered above the nursery pond that floating has seedlings nursing plate, keeps Natural lighting condition;
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5-11 days long to three leaves wholeheartedly, grown to three in Brassica rapa L Leaf wholeheartedly when to plant spray 500-1000 times of liquid of a Bravo;
(2)Worm kind is bred
Get out worm kind room, Flotage seedling raising pool be equipped in worm kind room, contained in the Flotage seedling raising pool in worm kind room it is good with Step(1)Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step(1)It is upper in 3-5 seedlings nursing plate of first sowing When extra large blueness plant grows to three leaves wholeheartedly, the 3-5 seedlings nursing plate is relayed in the Flotage seedling raising pool of worm kind room;
It acquires cigarette aphid and aphidius gifuensis respectively to field, the collected cigarette aphid in field and aphidius gifuensis is put into worm kind room simultaneously Three leaves wholeheartedly on the plant of Brassica rapa L, often discharge cigarette aphid 500-800, aphidius gifuensis 1-5 heads on disk Brassica rapa L plant, keep worm Indoor 15-30 DEG C of the temperature of kind, humidity 30-80%, aphidius gifuensis and cigarette aphid were in worm kind indoor common breeding 5-10 days, in worm kind Breeding obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis, the cigarette on Brassica rapa L plant leaf on indoor Brassica rapa L plant leaf Aphid reaches 10%-20% by the ratio of aphidius gifuensis parasitism, spare;
(3)Aphidius gifuensis Ashmaed scale is bred
By step(2)Breeding has tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to pick in worm kind room, uses The breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to be torn into length by the method for artificial sheet It is equably inoculated into step after 4-6cm, width 2-3cm(1)On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, aphid More inoculation blades is face-up, tobacco aphid kind 5-10, aphidius gifuensis worm kind 1-2 heads is inoculated on every plant of Brassica rapa L plant, in Buddhist nun Composite fertilizer's aqueous solution is added in Flotage seedling raising pool in imperial guard, controls the total nitrogen concentration in nylon guard in Flotage seedling raising pool and protects It holds in 200-400ppm, breeds 20-30 DEG C of temperature, be inoculated with up to a length of 4-6cm, inoculation blade that width is 2-3cm is clear after 2 days Except clean, connect worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf after 3 days, is sprayed one time 800 to Brassica rapa L plant Times carbendazol, it is 25 DEG C, humidity 50% to control breeding temperature in nylon guard, after continuing breeding 10 days, every plant of Brassica rapa L plant Upper obtained cigarette aphid quantity of breeding reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains nylon Composite fertilizer's aqueous solution in guard in Flotage seedling raising pool, the 11 of fine day:00-16:When 00 shading rate is covered above nylon guard For the sunshade net of 50%-70%, when Brassica rapa L plant is wilted into nursery pond injected clear water, control injected clear water depth 1- Then 2cm, 1 hour time drain the clear water in nursery pond, the matrix in seedlings nursing plate cultivation cave is kept to moisten Brassica rapa L plant not It wilts, drains and continue breeding after composite fertilizer's aqueous solution and reach 3-5 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches 150 or more, aphidius gifuensis reaches 95% or more to the parasitic rate of cigarette aphid, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more, It removes on Brassica rapa L plant not by the cigarette aphid of aphidius gifuensis parasitism, obtains numerous plant for having aphidius gifuensis deadlock aphid, it will be on plant Obtained aphidius gifuensis deadlock aphid is bred to carry out storage or numerous plant for having aphidius gifuensis deadlock aphid is directly used for field control aphid Evil, often disk is numerous has the plant of aphidius gifuensis deadlock aphid that can prevent farmland 10-15 mus of aphid evil.
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