CN105165313B - Aphidius gifuensis short cycle mating system - Google Patents
Aphidius gifuensis short cycle mating system Download PDFInfo
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- CN105165313B CN105165313B CN201510440277.0A CN201510440277A CN105165313B CN 105165313 B CN105165313 B CN 105165313B CN 201510440277 A CN201510440277 A CN 201510440277A CN 105165313 B CN105165313 B CN 105165313B
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- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 120
- 230000013011 mating Effects 0.000 title claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims abstract description 129
- 241001124076 Aphididae Species 0.000 claims abstract description 114
- 240000008100 Brassica rapa Species 0.000 claims abstract description 107
- 235000011292 Brassica rapa Nutrition 0.000 claims abstract description 106
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 63
- 238000009395 breeding Methods 0.000 claims abstract description 45
- 230000001488 breeding effect Effects 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000007667 floating Methods 0.000 claims abstract description 18
- 235000013311 vegetables Nutrition 0.000 claims abstract description 11
- 230000003071 parasitic effect Effects 0.000 claims abstract description 10
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 7
- 230000000474 nursing effect Effects 0.000 claims description 54
- 239000007864 aqueous solution Substances 0.000 claims description 36
- 239000004677 Nylon Substances 0.000 claims description 31
- 229920001778 nylon Polymers 0.000 claims description 31
- 238000009331 sowing Methods 0.000 claims description 21
- 239000003337 fertilizer Substances 0.000 claims description 20
- 239000002131 composite material Substances 0.000 claims description 16
- 241000721621 Myzus persicae Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 8
- 230000024241 parasitism Effects 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 208000035240 Disease Resistance Diseases 0.000 claims description 4
- 241001582888 Lobus Species 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 239000011159 matrix material Substances 0.000 claims description 4
- 238000003860 storage Methods 0.000 claims description 3
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 4
- 229910052757 nitrogen Inorganic materials 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 10
- 238000005516 engineering process Methods 0.000 abstract description 7
- 238000005265 energy consumption Methods 0.000 abstract description 5
- 230000001788 irregular Effects 0.000 abstract description 2
- 241000208125 Nicotiana Species 0.000 description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 7
- 230000002265 prevention Effects 0.000 description 6
- 230000027272 reproductive process Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 235000019994 cava Nutrition 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241001425390 Aphis fabae Species 0.000 description 1
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 241001674939 Caulanthus Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000180219 Sitobion avenae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021393 food security Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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- 235000009566 rice Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
Aphidius gifuensis short cycle mating system,Step includes host plant cultivation,Worm kind is bred,Aphidius gifuensis Ashmaed scale is bred,The overall process period is bred at 29 40 days,Often disk Brassica rapa L can educate to obtain cigarette aphid 12000 or more,Aphidius gifuensis parasitic rate 95%,Stiff aphid formation rate 60%,Often disk aphidius gifuensis deadlock aphid can be used to prevent 10 15 mu of farmland of aphid evil,Host plant is made using vegetable variety Brassica rapa L,It is adaptable,Growth and development is fast,It is the shortest host plant kind of cultivation period in current aphidius gifuensis raising technology,Substantially reduce the breeding cycle of aphidius gifuensis,Improve the breeding efficiency of aphidius gifuensis,Present invention application floating seedlings method cultivates host plant,Processing step is simple,Solves breeding high energy consumption,Techniqueflow is cumbersome,Aphidius gifuensis poor activity,Aphidius gifuensis deadlock aphid forms irregular problem,Aphidius gifuensis can be promoted in the widely applied utilization rate of agriculture and forestry,Aphidius gifuensis is set more to produce,The feasibility of popularization and application.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry pest, and in particular to a kind of aphidius gifuensis short cycle breeding
Method.
Background technology
Aphid is one of the primary pest in farm-forestry crop production, in leaf tobacco production the harm of aphid be particularly acute, it is main
Tobacco leaf juice is sucked on the tobacco leaf back of the body and tender stem with adult and nymph, cause cigarette strain to grow short and small, nutritional imbalance, influence cigarette
The normal growth and development of strain, the easy generation for inducing the diseases such as dark mildew of honeydew of aphid secretion, while aphid are also propagated a variety of
Tobacco virus causes the prevalence of tobacco virus；Mainly there are chemical prevention and physical control, chemical prevention to rely on agriculture aphid
Medicine eliminates aphid, bio-diversity can be caused to reduce, and destroys ecological environment, and pollution of agricultural products influences food security, and aphid resists
Pharmacological property enhances, and control effect declines, and physical control relies primarily on yellow plate prevention and control alatae worm, when alatae worm colonizes on plant
When, prevention and control effect that yellow plate has just scattered and disappeared to aphid.
In Agro-ecology development, biological prevention is anti-one of the most effective approach that eliminates aphis, aphidius gifuensis
It is the advantage parasite of cigarette aphid, is distributed throughout our country, ovum is originated in feeding juice in aphid body and killed by aphidius gifuensis
Dead or control aphid, breeds the offspring of oneself, it has good control effect to cigarette aphid, while to grain aphid, wheat y-bend
The aphids such as aphid, bean aphid, cotten aphid also have certain control effect, currently, aphidius gifuensis artificial breeding technology still locates study demonstration
In the stage, there are host plant kinds not to enrich, and host's feeding habits are single, cultivates difficult, aphidius gifuensis length breeding cycle, poor activity,
It is numerous to be badly in need of a kind of short-period aphidius gifuensis of stability and high efficiency of research for the problems such as aphidius gifuensis deadlock aphid formation regularity is poor, high energy consumption
Educate method.
Invention content
The present invention is in the prior art, and there are host plant kinds not to enrich for artificial breeding aphidius gifuensis, host's feeding habits
It is single, difficulty is cultivated, aphidius gifuensis length breeding cycle, poor activity, aphidius gifuensis deadlock aphid formation regularity is poor, high energy consumption, breeding
The problem of tedious process, host plant are easy to pass disease biography poison in vega, and aphid invades vega risk, it is short to provide a kind of aphidius gifuensis
Period mating system, the host plant that this method uses is vegetable variety Brassica rapa L, applies floating seedlings method and cultivates host's plant
Object, steps are as follows for specific breeding：
（1）Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis；
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps aqueous solution in nursery pond
Depth be 5-10cm, control nursery pond in aqueous solution a concentration of 150-250ppm of total N；
Floating seedlings special seedlings nursing plate 60-500 are got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, high by 5
Centimetre, each seedlings nursing plate is equipped with 80-162 cultivation cave, and seedling medium has been contained in the cultivation cave of seedlings nursing plate；
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first is in 3-5 seedlings nursing plate
Sowing is had 3-5 seedlings nursing plate of Brassica rapa L seed to relay and swum on the aqueous solution of nursery pond by middle sowing Brassica rapa L seed,
Floating has the nylon guard of covering 60-80 mesh above the nursery pond of seedlings nursing plate, natural lighting condition is kept, remaining after 10 days
Second batch sows Brassica rapa L seed in 55-597 seedlings nursing plate, and second batch sowing is had to 55-597 seedlings nursing plate of Brassica rapa L seed
It relays and swims on the aqueous solution of nursery pond, the nylon guard of 60-80 mesh is covered above the nursery pond that floating has seedlings nursing plate, protect
Hold natural lighting condition；
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5-11 days long to three leaves wholeheartedly, it is long in Brassica rapa L
500-1000 times of liquid of a Bravo is sprayed to plant when to three leaves wholeheartedly；
（2）Worm kind is bred
Worm kind room is got out, Flotage seedling raising pool is equipped in worm kind room, is contained in the Flotage seedling raising pool in worm kind room
Good and step（1）Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step（1）In 3-5 seedlings nursing plate of first sowing
Brassica rapa L plant when growing to three leaves wholeheartedly, the 3-5 seedlings nursing plate is relayed in the Flotage seedling raising pool of worm kind room；
It acquires cigarette aphid and aphidius gifuensis respectively to field, puts the collected cigarette aphid in field and aphidius gifuensis into worm kind simultaneously
Three leaves often discharge cigarette aphid 500-800, aphidius gifuensis 1-5 heads, guarantor wholeheartedly on the plant of Brassica rapa L in room on disk Brassica rapa L plant
15-30 DEG C of worm kind indoor temperature, humidity 30-80% are held, aphidius gifuensis and cigarette aphid breed 5-10 days jointly in worm kind interior,
It is bred on the indoor Brassica rapa L plant leaf of worm kind and obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis, on Brassica rapa L plant leaf
Cigarette aphid 10%-20% is reached by the ratio of aphidius gifuensis parasitism, it is spare；
（3）Aphidius gifuensis Ashmaed scale is bred
By step（2）There is under the picking of the Brassica rapa L plant leaf of tobacco aphid kind and aphidius gifuensis worm kind breeding in worm kind room
Come, manually the breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to tear by the method for sheet
It is equably inoculated into step after growth 4-6cm, width 2-3cm（1）On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard,
Inoculation blade more than aphid is face-up, and tobacco aphid kind 5-10, aphidius gifuensis worm kind 1-2 heads are inoculated on every plant of Brassica rapa L plant,
Composite fertilizer's aqueous solution is added in Flotage seedling raising pool in nylon guard, the total N controlled in nylon guard in Flotage seedling raising pool is dense
Degree is maintained at 200-400ppm, breeds 20-30 DEG C of temperature, be inoculated with up to after 2 days by a length of 4-6cm, the inoculation leaf that width is 2-3cm
Piece is removed totally, and connect worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf after 3 days, is sprayed to Brassica rapa L plant primary
800 times of carbendazols, it is 25 DEG C, humidity 50% to control breeding temperature in nylon guard, after continuing breeding 10 days, every plant of Brassica rapa L
The cigarette aphid quantity bred on plant reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains
Composite fertilizer's aqueous solution in nylon guard in Flotage seedling raising pool, the 11 of fine day:00-16:It is covered above nylon guard when 00
The sunshade net of 50%-70%, when Brassica rapa L plant is wilted into nursery pond injected clear water, control injected clear water depth 1-
Then 2cm, 1 hour time drain the clear water in nursery pond, the matrix in seedlings nursing plate cultivation cave is kept to moisten Brassica rapa L plant not
It wilts, drains and continue breeding after composite fertilizer's aqueous solution and reach 3-5 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches
150 or more, aphidius gifuensis reaches 95% or more to the parasitic rate of cigarette aphid, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more,
It removes on Brassica rapa L plant not by the cigarette aphid of aphidius gifuensis parasitism, obtains numerous plant for having aphidius gifuensis deadlock aphid, it will be on plant
Obtained aphidius gifuensis deadlock aphid is bred to carry out storage or numerous plant for having aphidius gifuensis deadlock aphid is directly used for field control aphid
Evil, often disk is numerous has the plant of aphidius gifuensis deadlock aphid that can prevent farmland 10-15 mus of aphid evil；
The host plant that the present invention uses is vegetable variety Brassica rapa L, further enriches host plant kind；Brassica rapa L
It is adaptable, it can be cultivated in most area Zhou Nianjun, solve the host plant of previous kind by natural conditions constraint, cultivation
Difficult problem；Brassica rapa L growth and development is fast, sows 3 days and emerges, and cultivates the host's plant that can be used as breeding aphidius gifuensis for 8-14 days
Object is the shortest host plant kind of cultivation period in current aphidius gifuensis raising technology, substantially reduces the numerous of aphidius gifuensis
Educate the period；Brassica rapa L is the host of cigarette aphid eating, and cigarette aphid growth reproduction speed on Brassica rapa L plant is fast, detoxification is quick, neat
Spend, activity is strong, individual is healthy and strong, Brassica rapa L adapts to the nutriment needed for the various cigarette aphid breeding such as tobacco type, wild cabbage, various
Cigarette aphid can be good at growth and development on Brassica rapa L, and excellent host can be provided for aphidius gifuensis, can prevent breeding and answer
Risk；
The present invention is proposed cultivates host plant Brassica rapa L using float seedling method, provides specific in cultivating process
, the technical parameter of strong operability, to breeding the illumination in each link, liquid manure technology requires to have carried out regulation and standardization control
System, makes aphidius gifuensis realize the large-scale production and application of batch production, commercialization, and aphidius gifuensis can be made to make in tobacco, vegetables
Popularization and application obtain remarkable effect in the productions such as object, while reducing aphidius gifuensis production cost, improve aphidius gifuensis in agriculture
The widely applied utilization rate of forestry makes aphidius gifuensis have more the feasibility for producing, promoting and applying, and cultivates host using the present invention and plants
Object can not only ensure that the nutritional condition of host plant is excellent, and saving of work and time, solve breeding aphidius gifuensis high energy consumption, skill
Art flow is cumbersome, aphidius gifuensis poor activity, aphidius gifuensis deadlock aphid form irregular problem；
The technology of the present invention covers nylon guard above nursery pond, and at low cost, flexibility is strong, can prevent remaining insect
Feeding to Brassica rapa L, while also solving the problems, such as cigarette aphid in reproductive process, aphidius gifuensis escape, it ensure that the safety of technology
Property, feasibility；Carbendazol in time is sprayed to host plant in reproductive process, ensure that host plants in aphidius gifuensis breeding
Object and the anti-characteristic of disease of cigarette aphid, aphidius gifuensis, can improve the safety in aphidius gifuensis reproductive process and stability, ensure that simultaneously
Carrying property, high efficiency of the host plant to aphidius gifuensis host cigarette aphid；Present invention process step is simple, simplifies previous breeding skill
The technical step bred respectively to cigarette aphid, aphidius gifuensis is needed in art, reduces the energy consumption in reproductive process, improves cigarette aphid cocoon
The breeding efficiency of bee.
Specific implementation mode
Embodiment 1：A kind of aphidius gifuensis short cycle mating system, the host plant that this method uses is vegetable variety Shanghai
Blueness applies floating seedlings method and cultivates host plant, and steps are as follows for specific breeding：
（1）Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis；
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps aqueous solution in nursery pond
Depth be 5cm, control nursery pond in aqueous solution a concentration of 150ppm of total N；
The special seedlings nursing plate of floating seedlings 60 is got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, 5 centimetres high,
Each seedlings nursing plate is equipped with 162 cultivation caves, and seedling medium has been contained in the cultivation cave of seedlings nursing plate；
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first is in 3 seedlings nursing plates
Brassica rapa L seed is sowed, there are 3 seedlings nursing plates of Brassica rapa L seed to relay sowing and swims on the aqueous solution of nursery pond, floating
The nylon guard for having 60 mesh of covering above the nursery pond of seedlings nursing plate, keeps natural lighting condition, is educated at remaining 57 after 10 days
Second batch sows Brassica rapa L seed in seed plate, has 57 seedlings nursing plates of Brassica rapa L seed to relay second batch sowing and swims in nursery
On the aqueous solution in pond, the nylon guard of 60 mesh is covered above the nursery pond that floating has seedlings nursing plate, keeps natural lighting condition；
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5 days long to three leaves wholeheartedly, Brassica rapa L grow to
Three leaves wholeheartedly when to plant spray 500 times of liquid of a Bravo；
（2）Worm kind is bred
Worm kind room is got out, Flotage seedling raising pool is equipped in worm kind room, is contained in the Flotage seedling raising pool in worm kind room
Good and step（1）Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step（1）In 3 seedlings nursing plates of first sowing
When Brassica rapa L plant grows to three leaves wholeheartedly, 3 seedlings nursing plates are relayed in the Flotage seedling raising pool of worm kind room；
It acquires cigarette aphid and aphidius gifuensis respectively to field, puts the collected cigarette aphid in field and aphidius gifuensis into worm kind simultaneously
Three leaves often discharge cigarette aphid 500, aphidius gifuensis 2 wholeheartedly on the plant of Brassica rapa L in room on disk Brassica rapa L plant, keep worm kind
15 DEG C of indoor temperature, humidity 30%, aphidius gifuensis and cigarette aphid were in the indoor common breeding of worm kind 10 days, in the indoor Shanghai of worm kind
Breeding obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis on green plant leaf, and the cigarette aphid on Brassica rapa L plant leaf is by cigarette aphid cocoon
The ratio of bee parasitism reaches 10%-20%, spare；
（3）Aphidius gifuensis Ashmaed scale is bred
By step（2）There is under the picking of the Brassica rapa L plant leaf of tobacco aphid kind and aphidius gifuensis worm kind breeding in worm kind room
Come, manually the breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to tear by the method for sheet
It is equably inoculated into step after growth 4cm, width 2cm（1）On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, aphid
More inoculation blades is face-up, tobacco aphid kind 10, aphidius gifuensis worm kind 2 is inoculated on every plant of Brassica rapa L plant, in nylon wire
Composite fertilizer's aqueous solution is added in Flotage seedling raising pool in cover, controls total N concentration in nylon guard in Flotage seedling raising pool and is maintained at
200ppm breeds 20 DEG C of temperature, is inoculated with clean up to removing a length of 4cm, the inoculation blade that width is 2cm after 2 days, is connect after 3 days
Worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf, sprays 800 times of carbendazols to Brassica rapa L plant, controls Buddhist nun
It is 25 DEG C, humidity 50% that temperature is bred in imperial guard, is continued after breeding 10 days, the cigarette aphid bred on every plant of Brassica rapa L plant
Quantity reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains Flotage seedling raising pool in nylon guard
Interior composite fertilizer's aqueous solution, the 11 of fine day:00-16:When 00 above nylon guard covering 50% sunshade net, Brassica rapa L plant
Into nursery pond, injected clear water, control injected clear water depth 1cm, are then drained in nursery pond 1 hour time when strain is wilted
Clear water, keep the matrix moistening Brassica rapa L plant in seedlings nursing plate cultivation cave not wilt, drain continue after composite fertilizer's aqueous solution it is numerous
It educates up to 5 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches 150 or more, parasitism of the aphidius gifuensis to cigarette aphid
Rate reaches 95% or more, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more, removes on Brassica rapa L plant not by aphidius gifuensis
Parasitic cigarette aphid, obtains numerous plant for having aphidius gifuensis deadlock aphid, the aphidius gifuensis deadlock aphid bred on plant is stored
Or directly numerous plant for having an aphidius gifuensis deadlock aphid is done harm to for field control aphid, the every numerous plant energy for having aphidius gifuensis deadlock aphid of disk
Enough prevention aphids do harm to 15 mu of farmland.
Embodiment 2：A kind of aphidius gifuensis short cycle mating system, the host plant that this method uses is vegetable variety Shanghai
Blueness applies floating seedlings method and cultivates host plant, and steps are as follows for specific breeding：
（1）Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis；
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps aqueous solution in nursery pond
Depth be 10cm, control nursery pond in aqueous solution a concentration of 250ppm of total N；
The special seedlings nursing plate of floating seedlings 500 is got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, 5 lis high
Rice, each seedlings nursing plate are equipped with 80 cultivation caves, seedling medium have been contained in the cultivation cave of seedlings nursing plate；
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first is in 5 seedlings nursing plates
Brassica rapa L seed is sowed, there are 5 seedlings nursing plates of Brassica rapa L seed to relay sowing and swims on the aqueous solution of nursery pond, floating
The nylon guard for having 80 mesh of covering above the nursery pond of seedlings nursing plate, keeps natural lighting condition, is educated at remaining 595 after 10 days
Second batch sows Brassica rapa L seed in seed plate, has 595 seedlings nursing plates of Brassica rapa L seed to relay to swim in and educate second batch sowing
On the aqueous solution of Miao Chi, the nylon guard of 80 mesh is covered above the nursery pond that floating has seedlings nursing plate, keeps natural lighting condition；
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5 days long to three leaves wholeheartedly, Brassica rapa L grow to
Three leaves wholeheartedly when to plant spray 1000 times of liquid of a Bravo；
（2）Worm kind is bred
Worm kind room is got out, Flotage seedling raising pool is equipped in worm kind room, is contained in the Flotage seedling raising pool in worm kind room
Good and step（1）Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step（1）In 5 seedlings nursing plates of first sowing
When Brassica rapa L plant grows to three leaves wholeheartedly, 5 seedlings nursing plates are relayed in the Flotage seedling raising pool of worm kind room；
It acquires cigarette aphid and aphidius gifuensis respectively to field, puts the collected cigarette aphid in field and aphidius gifuensis into worm kind simultaneously
Three leaves often discharge cigarette aphid 800, aphidius gifuensis 5 wholeheartedly on the plant of Brassica rapa L in room on disk Brassica rapa L plant, keep worm kind
30 DEG C of indoor temperature, humidity 80%, aphidius gifuensis and cigarette aphid were in the indoor common breeding of worm kind 5 days, in the indoor Brassica rapa L of worm kind
Breeding obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis on plant leaf, and the cigarette aphid on Brassica rapa L plant leaf is by aphidius gifuensis
Parasitic ratio reaches 10%-20%, spare；
（3）Aphidius gifuensis Ashmaed scale is bred
By step（2）There is under the picking of the Brassica rapa L plant leaf of tobacco aphid kind and aphidius gifuensis worm kind breeding in worm kind room
Come, manually the breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to tear by the method for sheet
It is equably inoculated into step after growth 6cm, width 3cm（1）On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, aphid
More inoculation blades is face-up, tobacco aphid kind 5, aphidius gifuensis worm kind 1 is inoculated on every plant of Brassica rapa L plant, in nylon guard
In Flotage seedling raising pool in composite fertilizer aqueous solution is added, control total N concentration in nylon guard in Flotage seedling raising pool and be maintained at
200-400ppm breeds 30 DEG C of temperature, be inoculated with it is clean up to removing a length of 6cm, the inoculation blade that width is 3cm after 2 days, after 3 days
Connect worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf, sprays 800 times of carbendazols, control to Brassica rapa L plant
It is 25 DEG C, humidity 50% that temperature is bred in nylon guard processed, after continuing breeding 10 days, on every plant of Brassica rapa L plant breeding obtain
Cigarette aphid quantity reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains floating in nylon guard and educates
Composite fertilizer's aqueous solution in Miao Chi, the 11 of fine day:00-16:The sunshade net for covering 50%-70% when 00 above nylon guard,
Into nursery pond, injected clear water, control injected clear water depth 2cm, are then drained 1 hour time when Brassica rapa L plant is wilted
Clear water in nursery pond keeps the matrix moistening Brassica rapa L plant in seedlings nursing plate cultivation cave not wilt, drains composite fertilizer's aqueous solution
After continue breeding up to 3 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches 150 or more, and aphidius gifuensis is to cigarette
The parasitic rate of aphid reaches 95% or more, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more, remove on Brassica rapa L plant not by
The cigarette aphid of aphidius gifuensis parasitism obtains numerous plant for having aphidius gifuensis deadlock aphid, the aphidius gifuensis deadlock aphid that will be bred on plant
It carries out storage or directly does harm to numerous plant for having aphidius gifuensis deadlock aphid for field control aphid, often disk is numerous aphidius gifuensis deadlock aphid
Plant can prevent aphid evil 10 mu of farmland.
Claims (1)
1. aphidius gifuensis short cycle mating system, it is characterised in that：The host plant that this method uses is vegetable variety Brassica rapa L,
It applies floating seedlings method and cultivates host plant, steps are as follows for specific breeding：
（1）Host plant is cultivated
The host plant that the vegetable variety Brassica rapa L of the strong cigarette aphid eating of the fast disease resistance of growth selection is bred as aphidius gifuensis；
Flotage seedling raising pool is got out, the aqueous solution of Common compound fertilizer is added in nursery pond, keeps the depth of aqueous solution in nursery pond
Degree is 5-10cm, and the total nitrogen concentration for controlling aqueous solution in nursery pond is 150-250ppm；
Floating seedlings seedlings nursing plate 60-500 is got out, the specification of seedlings nursing plate is 60 centimetres of length, 34 centimetres wide, 5 centimetres high, often
A seedlings nursing plate is equipped with 80-162 cultivation cave, and seedling medium has been contained in the cultivation cave of seedlings nursing plate；
Sow the seed of Brassica rapa L in two batches in the seedlings nursing plate for having contained seedling medium, first broadcasts in 3-5 seedlings nursing plate
Sowing is had 3-5 seedlings nursing plate of Brassica rapa L seed to relay and swims on the aqueous solution of nursery pond, floating by kind Brassica rapa L seed
The nylon guard for having covering 60-80 mesh above the nursery pond of seedlings nursing plate, keeps natural lighting condition, in remaining 55- after 10 days
Second batch sows Brassica rapa L seed in 597 seedlings nursing plates, has 55-597 seedlings nursing plate of Brassica rapa L seed to turn second batch sowing
Blow up floats on the aqueous solution of nursery pond, and the nylon guard of 60-80 mesh is covered above the nursery pond that floating has seedlings nursing plate, keeps
Natural lighting condition；
Brassica rapa L seed is emerged after sowing reaches 3 days, cultivated after emergence it is 5-11 days long to three leaves wholeheartedly, grown to three in Brassica rapa L
Leaf wholeheartedly when to plant spray 500-1000 times of liquid of a Bravo；
（2）Worm kind is bred
Get out worm kind room, Flotage seedling raising pool be equipped in worm kind room, contained in the Flotage seedling raising pool in worm kind room it is good with
Step（1）Identical composite fertilizer's aqueous solution in Flotage seedling raising pool, in step（1）It is upper in 3-5 seedlings nursing plate of first sowing
When extra large blueness plant grows to three leaves wholeheartedly, the 3-5 seedlings nursing plate is relayed in the Flotage seedling raising pool of worm kind room；
It acquires cigarette aphid and aphidius gifuensis respectively to field, the collected cigarette aphid in field and aphidius gifuensis is put into worm kind room simultaneously
Three leaves wholeheartedly on the plant of Brassica rapa L, often discharge cigarette aphid 500-800, aphidius gifuensis 1-5 heads on disk Brassica rapa L plant, keep worm
Indoor 15-30 DEG C of the temperature of kind, humidity 30-80%, aphidius gifuensis and cigarette aphid were in worm kind indoor common breeding 5-10 days, in worm kind
Breeding obtains the worm kind of cigarette aphid and the worm kind of aphidius gifuensis, the cigarette on Brassica rapa L plant leaf on indoor Brassica rapa L plant leaf
Aphid reaches 10%-20% by the ratio of aphidius gifuensis parasitism, spare；
（3）Aphidius gifuensis Ashmaed scale is bred
By step（2）Breeding has tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to pick in worm kind room, uses
The breeding that picking obtains is had tobacco aphid kind and the Brassica rapa L plant leaf of aphidius gifuensis worm kind to be torn into length by the method for artificial sheet
It is equably inoculated into step after 4-6cm, width 2-3cm（1）On the Brassica rapa L plant that second batch sowing is cultivated in nylon guard, aphid
More inoculation blades is face-up, tobacco aphid kind 5-10, aphidius gifuensis worm kind 1-2 heads is inoculated on every plant of Brassica rapa L plant, in Buddhist nun
Composite fertilizer's aqueous solution is added in Flotage seedling raising pool in imperial guard, controls the total nitrogen concentration in nylon guard in Flotage seedling raising pool and protects
It holds in 200-400ppm, breeds 20-30 DEG C of temperature, be inoculated with up to a length of 4-6cm, inoculation blade that width is 2-3cm is clear after 2 days
Except clean, connect worm kind moves to the back side or the lobus cardiacus of Brassica rapa L plant leaf after 3 days, is sprayed one time 800 to Brassica rapa L plant
Times carbendazol, it is 25 DEG C, humidity 50% to control breeding temperature in nylon guard, after continuing breeding 10 days, every plant of Brassica rapa L plant
Upper obtained cigarette aphid quantity of breeding reaches 100 or more, and aphidius gifuensis reaches 50% or more to the parasitic rate of cigarette aphid, drains nylon
Composite fertilizer's aqueous solution in guard in Flotage seedling raising pool, the 11 of fine day:00-16:When 00 shading rate is covered above nylon guard
For the sunshade net of 50%-70%, when Brassica rapa L plant is wilted into nursery pond injected clear water, control injected clear water depth 1-
Then 2cm, 1 hour time drain the clear water in nursery pond, the matrix in seedlings nursing plate cultivation cave is kept to moisten Brassica rapa L plant not
It wilts, drains and continue breeding after composite fertilizer's aqueous solution and reach 3-5 days, the cigarette aphid quantity bred on every plant of Brassica rapa L plant reaches
150 or more, aphidius gifuensis reaches 95% or more to the parasitic rate of cigarette aphid, and the stiff aphid formation rate of aphidius gifuensis reaches 60% or more,
It removes on Brassica rapa L plant not by the cigarette aphid of aphidius gifuensis parasitism, obtains numerous plant for having aphidius gifuensis deadlock aphid, it will be on plant
Obtained aphidius gifuensis deadlock aphid is bred to carry out storage or numerous plant for having aphidius gifuensis deadlock aphid is directly used for field control aphid
Evil, often disk is numerous has the plant of aphidius gifuensis deadlock aphid that can prevent farmland 10-15 mus of aphid evil.
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| CN105900931A (en) * | 2016-05-10 | 2016-08-31 | 云南省烟草公司玉溪市公司 | method for breeding Lipaphis erysimi on large scale |
| CN105900932B (en) * | 2016-05-10 | 2019-09-10 | 云南省烟草公司玉溪市公司 | A kind of method of efficient breeding radish aphid |
| CN106234312B (en) * | 2016-08-05 | 2019-02-26 | 云南省烟草公司昆明市公司 | A kind of save space, low cost, the aphidius gifuensis of economical and efficient for kind of a method |
| CN106259208B (en) * | 2016-08-08 | 2019-07-05 | 河南省烟草公司洛阳市公司 | A kind of method that tobacco plant repeatedly breeds aphidius gifuensis |
| CN106577549A (en) * | 2016-12-05 | 2017-04-26 | 云南省烟草公司丽江市公司 | Insect disease prevention and control method during the Myzus persi cae (Sulzer) breeding process |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN102388842A (en) * | 2011-08-04 | 2012-03-28 | 云南省烟草公司保山市公司 | Method for breeding and feeding Aphidius gifuensis Ashmesd in floating seedling raising shed |
| CN102812933A (en) * | 2012-09-11 | 2012-12-12 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN104642267A (en) * | 2015-02-09 | 2015-05-27 | 云南绿叶生防科技有限公司 | Industrial cultivation method for aphidius gifuensis ashmead |
| CN104756955A (en) * | 2015-03-11 | 2015-07-08 | 中国烟草总公司四川省公司 | Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method |
-
2015
- 2015-07-24 CN CN201510440277.0A patent/CN105165313B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN102388842A (en) * | 2011-08-04 | 2012-03-28 | 云南省烟草公司保山市公司 | Method for breeding and feeding Aphidius gifuensis Ashmesd in floating seedling raising shed |
| CN102812933A (en) * | 2012-09-11 | 2012-12-12 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN104642267A (en) * | 2015-02-09 | 2015-05-27 | 云南绿叶生防科技有限公司 | Industrial cultivation method for aphidius gifuensis ashmead |
| CN104756955A (en) * | 2015-03-11 | 2015-07-08 | 中国烟草总公司四川省公司 | Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method |
Non-Patent Citations (1)
| Title |
|---|
| 烟草漂浮育苗繁育烟蚜茧蜂的影响因素探讨;王夸平等;《浙江农业学报》;20130531;全文 * |
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