CN103385220B - Scale breeding method for encarsia formosa - Google Patents
Scale breeding method for encarsia formosa Download PDFInfo
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- CN103385220B CN103385220B CN201310313579.2A CN201310313579A CN103385220B CN 103385220 B CN103385220 B CN 103385220B CN 201310313579 A CN201310313579 A CN 201310313579A CN 103385220 B CN103385220 B CN 103385220B
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Abstract
The invention discloses a scale breeding method for encarsia formosa. The scale breeding method is finished in four greenhouses, namely a host plant cultivating room, a worm species room, an encarsia formosa species room and a breeding room, by steps. The scale breeding method comprises a host plant cultivating and managing step, a whitefly species managing step, a whitefly inoculating step, a whitefly nymph breeding step, an encarsia formosa species managing step, an encarsia formosa inoculating step, an encarsia formosa pupa breeding step, a pupa collecting step, a worm card or encarsia formosa box manufacturing and storing step and a whitefly controlling step. The scale breeding method has the advantages that process steps are continuously circulated and the encarsia formosa can be annually produced and bred. The scale breeding method is suitable for industrial scale production and reproduction of the encarsia formosa and is short in reproduction period, high in efficiency, low in energy consumption and low in cost. The obtained adult encarsia formosa, encarsia formosa pupa card or encarsia formosa box products are good in quality. The problem of insufficient supply for control over bemisia tabaci and trialeurodes vaporariorum in agriculture and forestry is solved.
Description
Technical field
The invention belongs to the biological prevention field of agriculture and forestry insect, be specifically related to the scale propagation method of the Encarsia formosa used in the process of biological control agriculture and forestry injurious insect.
Background technology
Aleyrodid is a kind of important pests of farm-forestry crop, all there is aleyrodid endangering to some extent the prodution and development of farm-forestry crop all over the world, aleyrodid is rasping-sucking mouthparts, adult and nymph suck water, in harm process, secrete a large amount of sweet liquid, bring out and propagate multiple mycosis, host range is extensive, harm flowers, fruit tree, medicinal material, 600 various plants of the Yi Baiduoge sections such as tobacco, aleyrodid population quantity is huge, fertility is strong, reproduction speed is fast, clustering is caused harm, killed plant leaf chlorisis, flavescence, wilt, blade and fruit are subject to polluting the rear quality that affects, when serious, cause plant withered.
For a long time, people mainly prevent and treat whitefly hazard by spraying the method for chemical pesticide, make the drug resistance of aleyrodid and pesticide resistance in continuous enhancing, the usage amount of chemical pesticide increases year by year, contaminated environment, threatens to the quality safety of agriculture and forestry product, affects the healthy of people.
Encarsia formosa is the natural enemy of aleyrodid, and Encarsia formosa produces its worm's ovum in the worm's ovum of aleyrodid, the nutrition that its larva sucks aleyrodid worm's ovum while growing up, thus aleyrodid is eliminated.It is an important subject of at present aleyrodid insect being carried out to biological control that application Encarsia formosa is eliminated aleyrodid.At present, the level of this research only develops into laboratory stage, and Encarsia formosa survival rate under artificial propagation condition is low, and the breeding cycle is long, can't realize large batch of annual supply, can not meet the green actual needs of producing of Modern Agriculture forestry.
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Summary of the invention
The present invention is directed in prior art, Encarsia formosa survival rate under artificial propagation condition is low, breeding cycle is long, can not realize large batch of annual supply, can not meet the problem of the green need of production of Modern Agriculture forestry, a kind of scale propagation method of Encarsia formosa is provided, and the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed in the booth of four of chambers substep and complete, and concrete steps are as follows:
(1) host plant is cultivated management
The host plant kind of select is grown seedlings, and the seedling of incubation is carried out potted plantly by batch being transplanted in host plant raising chamber, cultivates 30-40 days, standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
A little less than the kind of described host plant requires the strong resistance to insects of disease resistance, blade is many, large, and blade face is smooth, is beneficial to aleyrodid breeding and worm blocking work, is a kind of in flue-cured tobacco, eggplant, sweet potato;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without the large greenhouse whitefly of sick body or Bemisia tabaci adults, in worm kind chamber, put into the standby host plant of (1) step and raise aleyrodid, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 30～50 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 17～27 ℃ of temperature, RH.40～70%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 300～500, obtains aleyrodid worm kind;
Every 15-25 days, host plant in worm kind chamber is carried out to the old and new replaces, 20%～50% old host plant is shifted out to worm kind chamber, then from host plant raising chamber, fetch the new host plant that has 9-12 sheet true leaf and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that all has 9-12 sheet true leaf in worm kind chamber;
(3) aleyrodid inoculation
With the host plant of 9-12 sheet true leaf, be placed in worm kind chamber, aleyrodid lays eggs at the blade back of this host plant, host plant completes aleyrodid inoculation put 4-5 days in worm kind chamber after, after host plant inoculation aleyrodid worm's ovum, the host plant that has inoculated aleyrodid worm's ovum is put into breed and in chamber, breeds aleyrodid nymph;
Or in worm kind chamber, utilize portable cocoon honeybee to receive and catch the adult that device is collected aleyrodid, then the adult of aleyrodid is discharged into and is bred in chamber, the density that adult whitefly discharges in breeding chamber is every strain host plant 40-70 head, and adult whitefly is laid eggs and bred aleyrodid nymph breeding on indoor host plant;
(4) aleyrodid nymph breeds
Keeping breeding indoor temperature is 17-27 ℃, RH.40-70%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and within 5-8 days, to host plant, spray 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25%, continue to breed 10-15 days, the quantity that makes to breed the vacuum side of blade aleyrodid worm's ovum of indoor host plant is bred and is reached 10～40 every square centimeter, and has more than 50% aleyrodid worm's ovum to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in cultured insect bottle, after it is sprouted wings in cultivation bottle, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into honeybee kind indoor, the host plant that contains 5～20 2-3 age aleyrodid nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 17-27 ℃ of honeybee kind indoor temperature, RH.40-70%, after 14 days, in honeybee kind chamber, the density of the black pupa of Encarsia formosa reaches every strain host plant more than 2000, obtains the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 5～20 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, the black pupa of honeybee kind of gather to be about to sprouting wings, and the aleyrodid worm's ovum that is discharged into the vacuum side of blade of breeding host plant in chamber has on the host plant blade that more than 50% becomes aleyrodid nymph in 2～3 age;
Or in honeybee kind chamber, utilize portable cocoon honeybee to receive and catch the one-tenth honeybee that device is collected Encarsia formosa, the one-tenth honeybee that receipts are caught to the Encarsia formosa obtaining is discharged into step (4) breeding in chamber after finishing, Encarsia formosa becomes the burst size of honeybee and the quantity ratio of aleyrodid nymph in 2～3 age to be controlled at 1:20～40, and in breeding chamber, Encarsia formosa completes and connects honeybee;
(7) Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 17～27 ℃, RH.40～70% later, the suitable management of host plant liquid manure, cultivate 14～18 days, Encarsia formosa is bred in a large number, aleyrodid nymph 70%～85% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year obtain the black pupa of Encarsia formosa or become honeybee;
(8) bee pupa Collection and preservation
The aleyrodid nymph breeding in chamber becomes after the black pupa of Encarsia formosa, the blade that aleyrodid nymph more than 85% has been become to the host plant of black pupa collects, remove aleyrodid remaining on blade, obtain bee pupa blade, bee pupa blade is placed on to Indoor Natural air-dry 2～3 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, air-dry bee pupa blade is directly made to adult card;
Or the bee pupa on air-dry bee pupa blade is separated, pack into and in ventilating box, be made into honeybee box;
Worm card or honeybee box are stored in to 8～10 ℃ of temperature, in the refrigerating chamber of humidity 40～50%;
(9) aleyrodid control
Worm card or honeybee box are taken out from refrigerating chamber, before bee pupa is sprouted wings, render to the agricultural area control aleyrodid of whitefly hazard;
Or utilize portable cocoon honeybee to receive and catch the one-tenth honeybee that device is collected Encarsia formosa from breed chamber, will become honeybee to be discharged into the agricultural area control aleyrodid of whitefly hazard.
In processing step of the present invention, in the controlled canopy chamber of environmental condition, adopt host plant to cultivate management and the Encarsia formosa honeybee kind management method of management, aleyrodid worm kind, guarantee all to have in 1 year season host plant to raise the provenance of Encarsia formosa, guarantee that 1 year season all had honeybee kind to provide; In the present invention, the inoculation of the inoculation of aleyrodid and raising technology, Encarsia formosa and raising technology, can guarantee the whole year of Encarsia formosa, supply with in enormous quantities, and Encarsia formosa can successively, cyclically be produced and breed to these technology, the large-scale industrialized Reproduction that adapts to Encarsia formosa, its breeding cycle is short, aquaculture cost is low, the Encarsia formosa obtaining becomes honeybee and bee pupa worm card or honeybee box product quality superior, solved in agricultural production, prevent and treat the Encarsia formosa kind honeybee of Bemisia tabaci and greenhouse whitefly cannot be in batches, the problem supplied with continuously.Realized the industrialized development target that this biological prevention is applied from laboratory to land for growing field crops.
Embodiment
embodiment 1
The scale propagation method of Encarsia formosa, the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed in the booth of four of chambers substep and complete, and concrete steps are as follows:
(1) host plant is cultivated management
Select flue-cured tobacco and grow seedlings as host plant kind, the seedling of incubation is carried out potted plantly by batch being transplanted in host plant raising chamber, cultivate 30 days, standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without the large Bemisia tabaci adults of sick body, in worm kind chamber, put into the standby host plant of (1) step and raise aleyrodid, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 30 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 17 ℃ of temperature, RH.40%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 300～500, obtains aleyrodid worm kind;
Every 15 days, host plant in worm kind chamber is carried out to the old and new replaces, 20% old host plant is shifted out to worm kind chamber, then from host plant raising chamber, fetch the new host plant that has 9-12 sheet true leaf and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that all has 9-12 sheet true leaf in worm kind chamber;
(3) aleyrodid inoculation
With the host plant of 9-12 sheet true leaf, be placed in worm kind chamber, aleyrodid lays eggs at the blade back of this host plant, host plant completes aleyrodid inoculation put 4 days in worm kind chamber after, after host plant inoculation aleyrodid worm's ovum, the host plant that has inoculated aleyrodid worm's ovum is put into breed and in chamber, breeds aleyrodid nymph;
(4) aleyrodid nymph breeds
Keeping breeding indoor temperature is 17 ℃, RH.40%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and within 5 days, to host plant, spray 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25%, continue to breed 10 days, the quantity that makes to breed the vacuum side of blade aleyrodid worm's ovum of indoor host plant is bred and is reached 10 every square centimeter, and has more than 50% aleyrodid worm's ovum to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in cultured insect bottle, after it is sprouted wings in cultivation bottle, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into honeybee kind indoor, the host plant that contains 5 2-3 age aleyrodid nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 17 ℃ of honeybee kind indoor temperatures, RH.40%, after 14 days, in honeybee kind chamber, the density of the black pupa of Encarsia formosa reaches every strain host plant more than 2000, obtains the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 5 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, the black pupa of honeybee kind of gather to be about to sprouting wings, and the aleyrodid worm's ovum that is discharged into the vacuum side of blade of breeding host plant in chamber has on the host plant blade that more than 50% becomes aleyrodid nymph in 2～3 age;
(7) Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 17 ℃, RH.40% later, the suitable management of host plant liquid manure, cultivate 18 days, Encarsia formosa is bred in a large number, aleyrodid nymph 70% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year obtain the black pupa of Encarsia formosa or become honeybee;
(8) bee pupa Collection and preservation
The aleyrodid nymph breeding in chamber becomes after the black pupa of Encarsia formosa, the blade that aleyrodid nymph more than 85% has been become to the host plant of black pupa collects, remove aleyrodid remaining on blade, obtain bee pupa blade, bee pupa blade is placed on to Indoor Natural air-dry 2 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, air-dry bee pupa blade is directly made to adult card;
Worm card is stored in to 8 ℃ of temperature, in the refrigerating chamber of humidity 40%;
(9) aleyrodid control
Worm card is taken out from refrigerating chamber, before bee pupa is sprouted wings, render to the agricultural area control aleyrodid of whitefly hazard.
embodiment 2
The scale propagation method of Encarsia formosa, the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed in the booth of four of chambers substep and complete, and concrete steps are as follows:
(1) host plant is cultivated management
Select sweet potato and grow seedlings as host plant kind, the seedling of incubation is carried out potted plantly by batch being transplanted in cultivation canopy, cultivate 40 days, standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without the large greenhouse whitefly adult of sick body, in worm kind chamber, put into the standby host plant of (1) step and raise aleyrodid, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 50 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 27 ℃ of temperature, RH. 70%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 500, obtains aleyrodid worm kind;
Every 25 days, host plant in worm kind chamber is carried out to the old and new replaces, 50% old host plant is shifted out to worm kind chamber, then from host plant raising chamber, fetch the new host plant that has 9-12 sheet true leaf and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that all has 9-12 sheet true leaf in worm kind chamber;
(3) aleyrodid inoculation
In worm kind chamber, utilize portable cocoon honeybee to receive and catch the adult that device is collected aleyrodid, then the adult of aleyrodid is discharged into and is bred in chamber, the density that adult whitefly discharges in breeding chamber is 40 of every strain host plants, and adult whitefly is laid eggs and bred aleyrodid nymph breeding on indoor host plant;
(4) aleyrodid nymph breeds
Keeping breeding indoor temperature is 27 ℃, RH.70%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and within 8 days, to host plant, spray 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25%, continue to breed 15 days, the quantity that makes to breed the vacuum side of blade aleyrodid worm's ovum of indoor host plant is bred and is reached 40 every square centimeter, and has more than 50% aleyrodid worm's ovum to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in cultured insect bottle, after it is sprouted wings in cultivation bottle, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into honeybee kind indoor, the host plant that contains 20 2-3 age aleyrodid nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 27 ℃ of honeybee kind indoor temperatures, RH.70%, after 14 days, in honeybee kind chamber, the density of the black pupa of Encarsia formosa reaches every strain host plant more than 2000, obtains the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 20 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, utilize portable cocoon honeybee to receive and catch the one-tenth honeybee that device is collected Encarsia formosa, the one-tenth honeybee that receipts are caught to the Encarsia formosa obtaining is discharged into step (4) breeding in chamber after finishing, Encarsia formosa becomes the burst size of honeybee and the quantity ratio of aleyrodid nymph in 2～3 age to be controlled at 1:20, and in breeding chamber, Encarsia formosa completes and connects honeybee;
(7) Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 27 ℃, RH. 70% later, the suitable management of host plant liquid manure, cultivate 14 days, Encarsia formosa is bred in a large number, aleyrodid nymph 85% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year obtain the black pupa of Encarsia formosa or become honeybee;
(8) bee pupa Collection and preservation
The aleyrodid nymph breeding in chamber becomes after the black pupa of Encarsia formosa, the blade that aleyrodid nymph more than 85% has been become to the host plant of black pupa collects, remove aleyrodid remaining on blade, obtain bee pupa blade, bee pupa blade is placed on to Indoor Natural air-dry 3 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, the bee pupa on air-dry bee pupa blade is separated, pack into and in ventilating box, be made into honeybee box;
Honeybee box is stored in to 10 ℃ of temperature, in the refrigerating chamber of humidity 50%;
(9) aleyrodid control
Honeybee box is taken out from refrigerating chamber, before bee pupa is sprouted wings, render to the agricultural area control aleyrodid of whitefly hazard;
Utilize portable cocoon honeybee to receive and catch the one-tenth honeybee that device is collected Encarsia formosa from breed chamber, will become honeybee to be discharged into the agricultural area control aleyrodid of whitefly hazard.
Claims (2)
1. the scale propagation method of Encarsia formosa, is characterized in that: the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed substep in the booth of four of chambers and complete, and concrete steps are as follows:
(1) host plant is cultivated management
The host plant kind of select is grown seedlings, and the seedling of incubation is carried out potted plantly by batch being transplanted in host plant raising chamber, cultivates 30-40 days, standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without the large greenhouse whitefly of sick body or Bemisia tabaci adults, in worm kind chamber, put into the standby host plant of (1) step and raise aleyrodid, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 30～50 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 17～27 ℃ of temperature, RH.40～70%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 300～500, obtains aleyrodid worm kind;
Every 15-25 days, host plant in worm kind chamber is carried out to the old and new replaces, 20%～50% old host plant is shifted out to worm kind chamber, then from host plant raising chamber, fetch the new host plant that has 9-12 sheet true leaf and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that all has 9-12 sheet true leaf in worm kind chamber;
(3) aleyrodid inoculation
With the host plant of 9-12 sheet true leaf, be placed in worm kind chamber, aleyrodid lays eggs at the blade back of this host plant, host plant completes aleyrodid inoculation put 4-5 days in worm kind chamber after, after host plant inoculation aleyrodid worm's ovum, the host plant that has inoculated aleyrodid worm's ovum is put into breed and in chamber, breeds aleyrodid nymph;
Or in worm kind chamber, utilize portable cocoon honeybee to receive and catch the adult that device is collected aleyrodid, then the adult of aleyrodid is discharged into and is bred in chamber, the density that adult whitefly discharges in breeding chamber is every strain host plant 40-70 head, and adult whitefly is laid eggs and bred aleyrodid nymph breeding on indoor host plant;
(4) aleyrodid nymph breeds
Keeping breeding indoor temperature is 17-27 ℃, RH.40-70%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and within 5-8 days, to host plant, spray 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25%, continue to breed 10-15 days, the quantity that makes to breed the vacuum side of blade aleyrodid worm's ovum of indoor host plant is bred and is reached 10～40 every square centimeter, and has more than 50% aleyrodid worm's ovum to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in cultured insect bottle, after it is sprouted wings in cultivation bottle, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into honeybee kind indoor, the host plant that contains 5～20 2-3 age aleyrodid nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 17-27 ℃ of honeybee kind indoor temperature, RH.40-70%, after 14 days, in honeybee kind chamber, the density of the black pupa of Encarsia formosa reaches every strain host plant more than 2000, obtains the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 5～20 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, the black pupa of honeybee kind of gather to be about to sprouting wings, and the aleyrodid worm's ovum that is discharged into the vacuum side of blade of breeding host plant in chamber has on the host plant blade that more than 50% becomes aleyrodid nymph in 2～3 age;
Or in honeybee kind chamber, utilize portable cocoon honeybee to receive and catch the one-tenth honeybee that device is collected Encarsia formosa, the one-tenth honeybee that receipts are caught to the Encarsia formosa obtaining is discharged into step (4) breeding in chamber after finishing, Encarsia formosa becomes the burst size of honeybee and the quantity ratio of aleyrodid nymph in 2～3 age to be controlled at 1:20～40, and in breeding chamber, Encarsia formosa completes and connects honeybee;
(7) Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 17～27 ℃, RH.40～70% later, the suitable management of host plant liquid manure, cultivate 14～18 days, Encarsia formosa is bred in a large number, aleyrodid nymph 70%～85% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year obtain the black pupa of Encarsia formosa or become honeybee;
(8) bee pupa Collection and preservation
The aleyrodid nymph breeding in chamber becomes after the black pupa of Encarsia formosa, the blade that aleyrodid nymph more than 85% has been become to the host plant of black pupa collects, remove aleyrodid remaining on blade, obtain bee pupa blade, bee pupa blade is placed on to Indoor Natural air-dry 2～3 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, air-dry bee pupa blade is directly made to adult card;
Or the bee pupa on air-dry bee pupa blade is separated, pack into and in ventilating box, be made into honeybee box;
Worm card or honeybee box are stored in to 8～10 ℃ of temperature, in the refrigerating chamber of humidity 40～50%;
(9) aleyrodid control
Worm card or honeybee box are taken out from refrigerating chamber, before bee pupa is sprouted wings, render to the agricultural area control aleyrodid of whitefly hazard;
Or utilize portable cocoon honeybee to receive and catch the one-tenth honeybee that device is collected Encarsia formosa from breed chamber, will become honeybee to be discharged into the agricultural area control aleyrodid of whitefly hazard.
2. the scale propagation method of Encarsia formosa as claimed in claim 1, it is characterized in that: a little less than the kind of described host plant requires the strong resistance to insects of disease resistance, blade is many, large, and blade face is smooth, being beneficial to aleyrodid breeding and worm blocking work, is a kind of in flue-cured tobacco, eggplant, sweet potato.
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| CN104604809A (en) * | 2014-06-07 | 2015-05-13 | 云南省农业科学院农业环境资源研究所 | Biological control method using encarsia formosa for controlling tobacco whitefly |
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| CN110583581A (en) * | 2019-07-17 | 2019-12-20 | 中国农业科学院蔬菜花卉研究所 | Construction method of novel Bemisia tabaci nymph RNA interference method |
| CN110771577B (en) * | 2019-11-18 | 2021-09-24 | 西南林业大学 | High-density rapid breeding method for stiff aphids of aphidius gifuensis ashmeads |
| CN113180011B (en) * | 2021-05-07 | 2022-10-28 | 中山大学 | Large-scale breeding method for Anagrus nilaparvatae |
| CN114467580A (en) * | 2022-01-12 | 2022-05-13 | 山东潍坊烟草有限公司 | Insect prevention method for tobacco leaves |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2000007A (en) * | 1977-06-27 | 1979-01-04 | Koppert P | Article and Method for Combatting Harmful Insects in Horticulture |
| CN1631125A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Artificial progagation method for Encarsia formosa |
| CN101390505A (en) * | 2008-10-28 | 2009-03-25 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
| KR101044051B1 (en) * | 2010-12-23 | 2011-06-23 | 주식회사 세실 | Filler composition for breeding natural enemies, breeding composition for natural enemies, and controlling composition for insect pests comprising expanded chaff |
| CN102273432A (en) * | 2011-06-26 | 2011-12-14 | 西北农林科技大学 | Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host |
| CN102812933A (en) * | 2012-09-11 | 2012-12-12 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
-
2013
- 2013-07-25 CN CN201310313579.2A patent/CN103385220B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2000007A (en) * | 1977-06-27 | 1979-01-04 | Koppert P | Article and Method for Combatting Harmful Insects in Horticulture |
| CN1631125A (en) * | 2005-01-26 | 2005-06-29 | 北京市农林科学院 | Artificial progagation method for Encarsia formosa |
| CN101390505A (en) * | 2008-10-28 | 2009-03-25 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
| KR101044051B1 (en) * | 2010-12-23 | 2011-06-23 | 주식회사 세실 | Filler composition for breeding natural enemies, breeding composition for natural enemies, and controlling composition for insect pests comprising expanded chaff |
| CN102273432A (en) * | 2011-06-26 | 2011-12-14 | 西北农林科技大学 | Method for producing drone of light yellow encarsia amicula by taking encarsia formosa gahan as breeding host |
| CN102812933A (en) * | 2012-09-11 | 2012-12-12 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
Non-Patent Citations (6)
| Title |
|---|
| 丽蚜小蜂商品化生产技术;程洪坤等;《中国生物防治》;19891108(第04期);第178-181页 * |
| 丽蚜小蜂的繁殖技术;田毓起等;《农业科技通讯》;19820829(第08期);第21-22页 * |
| 曾凡荣.天敌昆虫饲养系统工程.《天敌昆虫饲养系统工程》.中国农业科学技术出版社,2009,(第1版),第226-230页. * |
| 林永范等.病虫害生防工作新进展.《我国农作物病虫害生防工作新进展》.中国农业出版社,1998,(第1版),第129-138页. * |
| 田毓起等.丽蚜小蜂的繁殖技术.《农业科技通讯》.1982,(第08期),第21-22页. |
| 程洪坤等.丽蚜小蜂商品化生产技术.《中国生物防治》.1989,(第04期),第178-181页. |
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