CN107333720A - A kind of method using temperature transition fast-propagation Hai Shi eretmocerus SPs - Google Patents
A kind of method using temperature transition fast-propagation Hai Shi eretmocerus SPs Download PDFInfo
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- 229920000742 Cotton Polymers 0.000 claims description 25
- 241000196324 Embryophyta Species 0.000 claims description 14
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- 240000002024 Gossypium herbaceum Species 0.000 claims description 7
- 235000004341 Gossypium herbaceum Nutrition 0.000 claims description 7
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The present invention relates to a kind of natural enemy insect fast-propagation technology, specifically relates to the method using temperature transition fast-propagation Hai Shi eretmocerus SPs.Methods described includes step:Cultivate host plant cotton;Bemisia tabaci;Hai Shi eretmocerus SPs are raised, the most suitable precocious stage developmental temperature of the raising of the Hai Shi eretmocerus SPs is 30 DEG C, and the spawning temperature in adult stage is 26 DEG C, the shading value L of suitable illumination:D=14:10, suitable humidity is 60~80%.The rearing method of the Hai Shi eretmocerus SPs of the present invention solves the proliferative speed that raising raising temperature can accelerate parasitic wasp, but the problem of reducing the reproductive efficiency of parasitic wasp, the extensive raising in interior of parasitic wasp is especially easy for, the fast-propagation of the honeybee can be achieved.
Description
Technical field
It is fast using temperature transition more particularly to one kind the present invention relates to a kind of local parasitic enemy insect method for breeding
The method that speed expands numerous Hai Shi eretmocerus SPs.
Background technology
Bemisia tabaci Bemisia tabaci (Gennadius), also known as whitefly in bt cotton or sweet potato whitefly etc., belong to Semiptera
Hemiptera Aleyrodidaes Aleyrodidae, small Aleyrodes, it is a kind of piercing-sucking small by feeding herbaceous plant bast
Insect.Developed rapidly due to drug-fast, Bemisia tabaci has turned into main on many National Cottons, vegetables and ornamental plants in garden
Insect.Increasingly tend to use in recent years based on biological control, Bemisia tabaci is carried out with reference to other prophylactico-therapeutic measures sustainable
The integrated control of property.
Hai Shi eretmocerus SP Eretmocerus hayati (Zolnerowich and Rose) be not only able to it is parasitic and
And can also direct feeding Bemisia tabaci Nymph, show superpower Biocontrol Potential.In order to carry out cigarette using Hai Shi eretmocerus SPs
Aleyrodid biological control, realizing the extensive raising of parasitic wasp becomes the problem of standing in the breach.
Existing parasitics and the vast propagation technology of predator are assessed with different steady temperatures mostly
Expansion numerous and biological and ecological methods to prevent plant disease, pests, and erosion efficiency influence of the temperature to natural enemy, comes how clear and definite temperature influences the problem of natural enemy is raised on a large scale with this.
However, in preference temperature section, developmental rate is proportional with temperature, and still, when temperature exceedes optimum temperature, developmental rate is also
It can continue to increase, but the ability such as the feeding of natural enemy, parasitism/predation declines, and have impact on the numerous speed of expansion of natural enemy, improves day
The cost of fast-propagation is opposed, hinders the process of natural enemy biological and ecological methods to prevent plant disease, pests, and erosion project.
The characteristics of present invention is divided into adult stage and precocious stage based on the growth cycle of parasitic wasp, it is to post to specify the adult stage
Raw honeybee is parasitic and the period of feeding host, precocious stage are that parasitic wasp completes ontogenetic period, and now parasitic wasp does not have
On the premise of parasitic or feeding host ability, the environment temperature for being expected that by metoxeny honeybee different developmental phases solves temperature
It is urgently so as to improve the pest controlling ability of parasitic wasp to changing the developmental rate of parasitic wasp and the restriction of its fecundity and feeding power
Solve the problems, such as.
The content of the invention
It is an object of the invention to provide a kind of method using temperature transition fast-propagation Hai Shi eretmocerus SPs.
Comprised the following steps according to the method using temperature transition fast-propagation Hai Shi eretmocerus SPs of the present invention:
(1) host plant cotton is cultivated;
(2) host plant for rearing Bemisia tabaci;
(3) Hai Shi eretmocerus SPs are raised,
Access Hai Shi eretmocerus SPs into honeybee to the cotton seedling for being loaded with Bemisia tabaci Nymph, be allowed to siphon away after spawning parasitic wasp into
Honeybee, continue to cultivate cotton plants at 30 ± 1 DEG C, after parasitic bee eclosion, environment temperature will be transferred into honeybee and continues to raise for 26 ± 1 DEG C
Support, wherein, the shading value L of illumination:D=14:10, humidity is 60~80%.
According to the embodiment of the present invention, the artificial extensive method for breeding of Hai Shi eretmocerus SPs includes following step
Suddenly:
(1) cultivation of host plant cotton:
Cotton seeds are seeded in the nutrition cup for be placed with nutrient matrix and cultivated, 2 seed/cups, the Nutrient medium
Matter is by vermiculite:Turf forms;Vermiculite:The volume ratio of turf is 1:1.5;Cotton seed germination and seedling growth periodically are checked, is protected
Hold the humidity of nutrient matrix in nutrition cup.Raising when cotton seedling plant is grown to 4 true leaves for Bemisia tabaci;
The cultivation of the host plant cotton is carried out in cleaning seedling room, ensures the pollution without any pest and disease damage, temperature 26 ± 1
DEG C, relative humidity 60~75%, illumination shading value is L:D=14:10.
(2) raising of host's Bemisia tabaci:
Cultured cotton plants are put into and connect in zooecium in the insect cage equipped with blue plastic casing, it is interior per cage to place 6 basins
Cotton seedling, 2 are pressed per cage:1 female-male proportion accesses 200 pairs of Bemisia tabaci adults;All Bemisia tabaci adults are removed after connecing worm 48 hours,
To obtain development age homogeneous Bemisia tabaci Nymph, continue cotton seedling strain of the culture with ovum, it is 26 ± 1 DEG C of temperature, relatively wet
Degree 60~80%, illumination shading value are L:D=14:10.
(3) Hai Shi eretmocerus SPs are raised
When Bemisia tabaci Nymph is developed to 2-3 ages after Bemisia tabaci is laid eggs 10~12 days, if a portion is loaded with into Bemisia tabaci
The cotton seedling of worm moves on in Bemisia tabaci insectary together with insect cage to be continued to raise, for Bemisia tabaci adults worm sources of future generation;It is remaining
The partial cotton seedling for being loaded with Bemisia tabaci Nymph moves to bee-keeping room with insect cage together, and parasitic wasp is cultivated as host;Pressed per cage
1:1 sex ration accesses first emergence Hai Shi eretmocerus SP of 50 pairs of emergence times no more than 48 hours into honeybee, lays eggs 48 hours
After remove parasitic wasp into honeybee.Afterwards, cotton plants are moved into high temperature insect cage together with insect cage, it is 30 ± 1 DEG C of temperature, relative
Humidity 60~80%, illumination shading value are L:D=14:10, continue to cultivate cotton plants, by 10 days or so cotton leaf backs
The milk yellow Hai Shi eretmocerus SPs pupa for having more than 90% occurs, and now a part of parasitic bee pupa can be fabricated to honeybee card and be sent to
Bemisia tabaci causes harm area, and another part pupa can continue raising until sprouting wings, and parasitic wasp is moved into room temperature insectary, temperature after emergence
26 ± 1 DEG C, relative humidity 60~80%, illumination shading value be L:D=14:10, for parasitic wasp ovipositing hosts, further breed down
A generation.
The positive beneficial effect of the present invention is as follows:
(1) rearing method of Hai Shi eretmocerus SPs of the invention, method is simple, easily grasps, is especially easy for room
It is interior to raise and observe its history of life, even if keeper does not have relevant professional knowledge, can also learn in a short time;Supporting worm frame can divide
Laminar is raised, and is taken up space smaller, and feeding cost is relatively low, after release field control Bemisia tabaci, can bring huge economic benefit, society
Can benefit and ecological benefits;
(2) method of the invention can quick large-scale breeding Hai Shi eretmocerus SPs, the honeybee can be realized indoors throughout the year
Continued propagation, can not only meet the needs of experimental study, it is often more important that can in short term realize and be particularly to each Bemisia tabaci generating region
Vegetables and organic agriculture demonstration area provide a large amount of natural enemy worm sources, are the green prevention and control power-assisted of aleyrodid class pest.
(3) present invention is joined by comparing development individual in Hai Shi eretmocerus SP Suitable ranges and Population life-table
Several changes, being divided into according to parasitic wasp has biological and ecological methods to prevent plant disease, pests, and erosion ability (adult stage) and the characteristics of without biological and ecological methods to prevent plant disease, pests, and erosion ability (precocious stage), by turning
The environment temperature in two stages is changed, shortens the development time of precocious stage, the parasitism and feeding efficiency in adult stage is improved, significantly carries
The biological and ecological methods to prevent plant disease, pests, and erosion efficiency and the numerous speed of expansion of the high parasitic wasp, the efficiency of the such parasitic wasp of indoor vast propagation is greatly facilitated.
Embodiment
The Hai Shi eretmocerus SPs that embodiment 1 is manually raised on a large scale
(1) basic facilities
Insectary:Quantity demand 4, it is respectively used to raise the cultivation of cotton cleaning seedling, Bemisia tabaci raising, parasitic honeybee kind
Group maintains raising and the raising of parasitic wasp fast-propagation, and area is depending on raising scale, general 10~20m2It is advisable, as parasitic wasp needs
Ask big, the quantity of insectary can be increased.The stainless steel screen window of window installation, and a ventilating fan work ventilation is installed and used and one
Temperature in 1.5 intelligent frequency-conversion airconditioning control insectaries;The laminar of varying number is put in insectary according to real area size
Worm frame is supported, the switch time of fluorescent tube on all foster worm framves in insectary is automatically controlled by KG316T microcomputer time-controlled switchs;
Support worm frame:240cm × 50cm × 200cm foster worm frame several, each shelf points 3 layers, every layer of bottom pads one layer
The fluorescent lamp that 6 specifications are 28W is installed at the thicker transparent plastic sheet of quality, top;Worm frame is supported with insectary, winter conservation room to match somebody with somebody
Set, each support each layer fluorescent lamp of worm frame and one hand switch is installed respectively;
Insect cage:The insect rearging cage of cuboid, specification:60cm × 40cm × 60cm, silk screen is firm, transparent, is easy to see
Examine;There are the big opening of slide fastener control and the small opening of hand-operated binding in insect cage front, and convenient sampling, cage quality is 100-120
Mesh nylon gauze.
(2) suitable condition of host's Bemisia tabaci raising
Temperature, humidity and illumination condition:Insectary keeps 26 ± 1 DEG C of temperature, and humidity is 60~80% degree, and (65% is most
It is good);Illumination shading value L:D=14:10 and 3500lux illumination condition;
Host's condition:The cotton seedling of 4~6 true leaves, since sowing, obtained after 30 days;
Individual plant cotton supports worm amount:20-30 is suitable to adult;
(3) suitable condition of Hai Shi eretmocerus SPs raising
Temperature, humidity and illumination condition:Insectary keeps 26 ± 1 DEG C of temperature, and relative humidity is 60~80% degree (65%
It is optimal);Illumination shading value L:D=14:10 and 3500lux illumination condition;
Host's condition:The Bemisia tabaci Nymph in 2-3 ages;
Individual plant cotton supports worm amount:4~6 pairs of adults are suitable;
(4) preparation of worm sources
Bemisia tabaci:The worm sources of raising can adopt from the main host plants crop such as field tomato, eggplant, cucumber, cotton for the first time
Collect nymph or adult, worm sources afterwards connect in the room of insectary for conservation, select the healthy and strong imago breeding of individual to be raised in insect
Support standby in cage.
Hai Shi eretmocerus SPs:If first time worm sources can gather from Xinjiang Uygur Autonomous Regions Turfan Prefecture cotton field
Worm or pupa, worm sources afterwards connect for conservation in the room of insectary.The imago breeding of conservation is in the insect rearging cage for having Bemisia tabaci
It is interior standby.
(5) standardization of host plant cotton is cultivated
Kind:Middle plant cotton No.1, other can also be used similar to cotton variety, to the survival rate of Bemisia tabaci without showing between kind
Write difference.
Sowing:By the end of January to by the end of December, broadcast every 7 days and once plant, to ensure to support the whole year host necessary to worm, cotton is main
Sowing is isolated in illumination greenhouse, and with insect cage, to prevent other insects from causing harm to seedling strain;During sowing, directly by cotton
Seed is seeded in black nutritive cup, and nutrition cup is placed in the plastic casing of square uncovered, and box specification is:65cm×35cm×
8cm, can select the box of different model according to the number of animals raised, and each nutrition cup first fills the compost of 2/3rds volumes, vertically broadcast
Kind of two seeds, down, half is embedded in compost seed tip, covers a thin layer nutrition again afterwards, using cover completely seed as
Preferably;Sowing pours enough water in first day, to be advisable up to 2/3rds plastic casings;Afterwards, water twice is poured weekly;Treat that seedling strain is long to 4
Raising during~6 true leaves for Bemisia tabaci;
(6) raising of Bemisia tabaci
Bemisia tabaci adults are laid eggs:The cotton that plastic nutritional cup is cultivated is put into insect rearging cage, 6~9 basins is put per cage, connects
Enter adult, 2 are pressed per cage:1 female-male proportion accesses 150~200 pairs of Bemisia tabaci adults;All Bemisia tabacis are removed after connecing worm 48 hours
Adult, continue cotton of the culture with ovum, 26 ± 1 DEG C of temperature, relative humidity 60~80%, L:D=14:10.After 10~12 days
Raising when Bemisia tabaci Nymph was developed to for 2 age for Hai Shi eretmocerus SPs.
(7) raising of Hai Shi eretmocerus SPs
The cultured cotton with Bemisia tabaci 2-3 age nymphs is moved into bee-keeping room, 8~10 plants are placed in each insect cage
Miao Zhu, it is 2 that 50 pairs of male and female sex ratios are accessed into each insect cage:1 Hai Shi eretmocerus SP adults, it is allowed to spawning 48 hours
Parasitic bee imago is removed afterwards.Afterwards, cotton plants are moved into high temperature insect cage, 30 ± 1 DEG C of temperature, phase together with insect cage
It is L to humidity 60~80%, illumination shading value:D=14:10, continue to cultivate cotton plants, carried on the back by 10 days or so cotton leafs
Portion is that the milk yellow Hai Shi eretmocerus SPs pupa for having more than 90% occurs, and now a part of parasitic bee pupa can be fabricated to honeybee card hair
Cause harm area toward Bemisia tabaci, another part pupa can continue raising until sprouting wings, and parasitic wasp is moved into room temperature insectary, temperature after emergence
26 ± 1 DEG C of degree, relative humidity 60~80%, illumination shading value are L:D=14:10, for parasitic wasp ovipositing hosts, further breed
It is of future generation.
(8) year output of Hai Shi eretmocerus SPs
Due to being up to 12 days in 26 DEG C of Xia Haishi eretmocerus SP Adult worms producting eggs phases, per going through from ovum to adult eclosion from generation to generation
Only 14 days phase, therefore annual interior can raise for 12 generations.The so annual producible parasitic wasp amount in separate room insectary is at least 2,320,000
Head.
Embodiment 2 changes parasitic wasp nymphal development and the temperature of Adult worms producting eggs phase improves the vast propagation speed of parasitic wasp
Parasitic wasp can be generally divided into precocious stage and adult stage, and precocious stage generally comprises ovum phase, nymph and pupa time.
Precocious stage mainly completes the ontogeny of parasitic wasp, to host and does not have pest controlling ability;And the adult stage mainly by feeding,
It is parasitic that pest controlling is caused to host with directly lethal.Parasitic wasp is to the pest controlling ability of host typically by its intrinsic rate of increase (r) come body
It is existing, and intrinsic rate of increase depends primarily on the length of the precocious stage of parasitic wasp and the fecundity in adult stage.Temperature can change
Its fecundity and feeding power can also be influenceed simultaneously by becoming the developmental rate of parasitic wasp.Therefore, how by changing parasitic wasp difference
The temperature of stage of development is come to improve the pest controlling ability of parasitic wasp be the purpose of the present invention.
The environment temperature of parasitic wasp precocious stage is referred to as developmental temperature, the environment temperature in adult stage is referred to as temperature of laying eggs
Degree.Study the influence that temperature parasitic wasp expands numerous speed and efficiency.
(1) influence of different development and the spawning temperature to the Hai Shi eretmocerus SP history of life and life table parameters
Study it is different development and spawning temperature conditionss (22+22,26+26,30+30 and 34+34 DEG C, relative humidity 60~
80%, illumination shading value L:D=14:10) to the influence of Hai Shi eretmocerus SP biological characteristicses.As shown in table 1, as a result table
Bright, the development duration of Hai Shi eretmocerus SPs is raised and shortened with temperature, and under 22 and 34 DEG C of constant temperatures, the honeybee queen bee is parasitic
The precocious stage for the Bemisia tabaci raised on cotton is respectively 18.8 days and 10.9 days, the life-span in queen bee adult stage raise with temperature and
Shorten, the time-to-live in adult stage is respectively 15.5 days and 4.9 days.Meanwhile parasitic wasp has maximum under the conditions of 26+26 DEG C
Spawning and food ingestion, and highest reproductive efficiency, host's lethal efficiency, but be difficult to take into account intrinsic rate of increase, intrinsic increasing
Long rate only arranges the 2nd (being shown in Table 2).
The different developmental temperature Xia Haishi eretmocerus SPs of table 1 are (average as the development duration of host using Bemisia tabaci 2-3 ages nymph
Value ± standard error)
Different letters represent significant difference (the HSD inspections) in 0.05 level after same row
The history of life of the different developments of table 2 and spawning temperature Xia Haishi eretmocerus SPs using Bemisia tabaci 2-3 ages nymph as host
(average value ± standard error)
Table 2 is continuous
Different letters represent significant difference (the HSD inspections) in 0.05 level after same row
(2) influence of different developmental temperatures and identical spawning temperature to the Hai Shi eretmocerus SP history of life and life table parameters
As shown in table 3, different developments and identical spawning temperature conditionss (22+26,26+26,30+26 and 34+26 be have studied
DEG C, relative humidity 60~80%, illumination shading value L:D=14:10) the influence research to Hai Shi eretmocerus SP biological characteristicses
As a result showing, the development duration of Hai Shi eretmocerus SPs is raised and shortened with temperature, but because the spawning temperature in adult stage is equal
For 26 DEG C, the life-span in queen bee adult stage raises and shortened with developmental temperature, but amplitude is significantly smaller than different developmental temperatures and production
Ovum Temperature Treatment, wherein, under 34+26 DEG C of processing, the queen bee life-span is significantly longer than 34+34 DEG C of processing.Generally speaking, it is different
At a temperature of developmental temperature and spawning, under different developmental temperatures and identical spawning temperature conditionss, the egg laying amount of parasitic wasp, feeding
Amount, reproductive efficiency, host's control efficiency and intrinsic rate of increase are all improved to some extent, parasitic under wherein 30+26 DEG C processing
The egg laying amount and reproductive efficiency of honeybee significantly increase, and this shows to improve developmental temperature to a certain extent, can accelerate parasitic wasp
Developmental rate, increase it and expand numerous efficiency.In addition, in this Temperature Treatment series, parasitic wasp has under the conditions of 30+26 DEG C
Maximum intrinsic rate of increase (being shown in Table 3).It can be seen that selecting 30 DEG C of developmental temperature in parasitic wasp precocious stage, the adult stage selects 26 DEG C
Spawning temperature, the numerous efficiency of expansion of parasitic wasp can be effectively improved.
The history of life of the different developments of table 3 and spawning temperature Xia Haishi eretmocerus SPs using Bemisia tabaci 2-3 ages nymph as host
(average value ± standard error)
Table 3 is continuous
(3) influence of identical developmental temperature and different egg temperature to the Hai Shi eretmocerus SP history of life and life table parameters
As shown in table 4, identical development and different egg temperature conditionss (26+22,26+26,26+30 and 26+34 be have studied
DEG C, relative humidity 60~80%, illumination shading value L:D=14:10) the influence research to Hai Shi eretmocerus SP biological characteristicses
As a result show, because the developmental temperature of precocious stage is 26 DEG C, the development durations of Hai Shi eretmocerus SPs does not have difference, but with
Lay eggs temperature rise, parasitic wasp egg-laying time, egg laying amount, food ingestion, the female drone life-span, feeding efficiency, host's control efficiency and
Intrinsic rate of increase all shows the trend similar from different developments and spawning Temperature Treatment, and most of parameter is all into increased
Trend, but increased amplitude is less than different developmental temperatures and identical spawning Temperature Treatment.This is also from side illustration, to parasitic wasp
For the history of life, spawning temperature is bigger to its influence power than developmental temperature.
The data of each group Temperature Treatment are combined and seen, parasitic wasp has maximum reproductive efficiency, host at 30+26 DEG C
Control efficiency and intrinsic rate of increase.Compared to 26+26 DEG C processing (the most suitable processing at a temperature of different developmental temperatures and spawning) is parasitic
Reproductive efficiency, host's control efficiency and the intrinsic rate of increase of honeybee increase by 3.2%, 7.5% and 18.7% respectively;Compared to 30+30 DEG C
Processing, reproductive efficiency, host's control efficiency and the intrinsic rate of increase of parasitic wasp increase by 28%, 22.8% and 7.2% respectively.This table
Bright, special according to parasitic wasp history of life feature (precocious stage is without reproduction and host's control ability), by the feeding of parasitic wasp
Foster temperature is divided into the developmental temperature of precocious stage and the spawning temperature in adult stage, and targetedly chooses different developmental temperatures
With spawning temperature, the numerous speed of expansion and efficiency of parasitic wasp can be significantly improved, it is short during this can be Bemisia tabaci biological control
The requirement for discharging a large amount of parasitic wasps is needed to provide support in time.
The identical development of table 4 and life of the different egg temperature Xia Haishi eretmocerus SPs using Bemisia tabaci 2-3 ages nymph as host
History (average value ± standard error) living
Table 4 is continuous
Claims (4)
- A kind of 1. method using temperature transition fast-propagation Hai Shi eretmocerus SPs, it is characterised in that methods described include with Lower step:(1) host plant cotton is cultivated;(2) host plant for rearing Bemisia tabaci;(3) Hai Shi eretmocerus SPs are raised,Hai Shi eretmocerus SPs are accessed into honeybee to the cotton seedling for being loaded with Bemisia tabaci Nymph, are allowed to siphon away after spawning parasitic wasp into honeybee, Continue to cultivate cotton plants at 30 ± 1 DEG C, after parasitic bee eclosion, environment temperature will be transferred into honeybee and continues to raise for 26 ± 1 DEG C, Wherein, the shading value L of illumination:D=14:10, humidity is 60~80%.
- 2. the method according to claim 1 using temperature transition fast-propagation Hai Shi eretmocerus SPs, it is characterised in that In step (1), cultivating the nutrient matrix of cotton includes vermiculite:Turf;Vermiculite:The volume ratio of turf is 1~1.5:1, treat cotton Raising during flower seedling plants length to 3~4 true leaves for Bemisia tabaci.
- 3. the method according to claim 1 using temperature transition fast-propagation Hai Shi eretmocerus SPs, it is characterised in that In step (3), each insect cage presses 2:1 female-male proportion accesses 200 pairs of Bemisia tabaci adults.
- 4. the method according to claim 1 using temperature transition fast-propagation Hai Shi eretmocerus SPs, it is characterised in that In step (3), each insect cage presses 1:1 sex ration accesses 50 pairs of Hai Shi eretmocerus SPs into honeybee.
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