CN106472431B - A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum - Google Patents
A kind of collection method of Serangium japonicum pupa and the artificial large-scale breeding method of Serangium japonicum Download PDFInfo
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- CN106472431B CN106472431B CN201610924333.2A CN201610924333A CN106472431B CN 106472431 B CN106472431 B CN 106472431B CN 201610924333 A CN201610924333 A CN 201610924333A CN 106472431 B CN106472431 B CN 106472431B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The present invention provides a kind of collection method of Serangium japonicum pupa, including obtains Ladybird egg, raising children phase ladybug, collects ladybug pupa.The method of the present invention is sustainable to obtain the relatively uniform Ladybird egg of development condition, makes circle of pupating using the convenient single face corrugated board of material source, production method is easy, and it is easy that pupa collects operating process；For the prior art, realizes Serangium japonicum and efficiently, quickly, in high volume breed, have the advantages that scientific and reasonable, simple, convenient and practical.
Description
Technical field
The invention belongs to insect reproduction fields, and in particular to a kind of collection method of Serangium japonicum pupa and Japanese nose angle
The artificial large-scale breeding method of ladybug.
Background technique
The breeding and utilization of natural enemy insect are the important means of the sustainable ecological management of pest, using indoor mass propagation day
Insect is opposed, merchandized handling and application are carried out, is to replace and reduce chemical pesticide use, protection environment, protection bio-diversity,
Improve one of agricultural output and the main path of quality.
Serangium japonicum is the important local predator of Bemisia tabaci, can prey on tobacco powder nit, each age nymph, can also
Bemisia tabaci adults, and grandis adults addiction tobacco powder nit are preyed on, therefore the growth of Bemisia tabaci quantity can be efficiently controlled,
Be conducive to control Bemisia tabaci before wildness causes harm, this acquires a special sense in biological control.Natural enemy wooden dipper is introduced with foreign countries
Worm is compared, which has had very strong adaptability to South China, China geographical environment and weather conditions, and the ladybug catches
Heavy (being up to more than 700 to the day predatory number of tobacco powder nit), adult circles in the air, transfer ability of creeping is strong, and Life of Adult is long, numerous
It is strong to grow power, breeding cycle generation is shorter, provides possibility for large scale artificial breeding and release application.
It there is no systematic research in terms of raising in Serangium japonicum room at present, be to acquire field Japan nose angle mostly
Based on ladybug, and raise indoors for completing to test in a short time, lack to indoor long-term, Steady breed Serangium japonicum into
Row research, more unstable indoor worm sources cause Serangium japonicum large scale artificial breeding and release application cannot achieve, because
This, a kind of method for being badly in need of establishing indoor large-scale breeding Serangium japonicum.
However, the collection of pupa is to restrict Serangium japonicum manually to advise greatly during Serangium japonicum indoor propagation
One significant obstacle of mould breeding because Serangium japonicum mature larva like to be clustered in old leaf blade back, plastic tub alms bowl edge it is recessed
Other hidden corners are pupated in slot or in insectary, more dispersed, not easy-to-search and collection.It there is no at present and effectively carry out day
The correlation technique that this Serangium japonicum pupa is collected, therefore also cannot achieve indoor large-scale breeding Serangium japonicum.
Summary of the invention
According to the habit of pupating of Serangium japonicum, circle of pupating is made using single face corrugated board in the present inventor,
By reasonable employment, can not only effectively collect ladybug pupa, realize the centralized collection of pupa, the concentration of adult sprout wings, mating and oviposition,
And the concentration raising of larval phase, and the neat each worm state ladybug of development can be obtained, and because single face corrugated board is easy to get,
Pupating, it is simple for production to enclose, reusable, and pupa collection operating process is very convenient, can grind for Serangium japonicum related biological
Study carefully and artificial large-scale breeding provides important technical support.
I.e. the first object of the present invention is to provide a kind of collection of Serangium japonicum pupa that operation is easy and efficient is applicable in
Method, comprising the following steps:
A. it obtains Ladybird egg: the host plant for infecting Bemisia tabaci being moved into grandis adults rearging cage, collects egg-laying season ladybug
Female adult pest is in the produced Ladybird egg in the host's blade back side；
B. raise children phase ladybug: step a, which is produced, has the host plant of Ladybird egg to move into the raising of ladybug receptacle；
C. it collects ladybug pupa: when ladybug children phase (ovum phase+larval phase) grows to the 12nd day, being put in host's plant basal part of stem
Set circle of pupating；When children phase (ovum phase+larval phase) grows to the 14th~15 day, the circle that will pupate is taken out, and is placed in grandis adults raising
In cage.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, in the ladybug female adult pest of the step a
The host plant infected with Bemisia tabaci is replaced in egg-laying season daily, it is sustainable to obtain the relatively uniform Ladybird egg of development condition.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the feeding of the children phase ladybug of the step b
Supporting the time is 14~15d.
In the present invention, term " corrugated paper " is by the corrugated paper of sheet lining and the waveform formed by corrugated roller processing
The plate object being bonded.Term " corrugated board (corrugated fiberboard) " refers to by one or more layers corrugated paper
The combination board being bonded between several layers Paper or cardboard.Single face corrugated board refers to by one or two layers cardboard or paper, with one
The corrugated board that layer corrugated paper is bonded.Parameter index in relation to single face corrugated board can refer to " corrugated paper board standard GB/T/
T6544-2008》。
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the step c's pupates circle to use
Single face corrugated board is made.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, it is described pupate circle for using it is long by 45~
The single face corrugated board of the strip of 50cm, wide 6~7cm is made；It is highly preferred that it is described pupate circle for using long 45~50cm,
The single face corrugated board of the strip of wide 6~7cm is surround along host's plant basal part of stem, and is fixed into cylindrical circle of pupating；More
Preferably, the circle of pupating is using made of single face corrugated board, around the circle of host plant 3~4, height 6~7cm, inner ring
3~4cm of diameter, outer ring 6~8cm of diameter cylinder pupate circle；Most preferably, the circle of pupating is placed in matrix in host's plant basin alms bowl
On Nutrition Soil, one layer of preservative film is first padded on Culture media in host's plant basin alms bowl before circle is pupated in placement.
Preferably, in the collection method of Serangium japonicum pupa of the present invention, the host of the infection Bemisia tabaci plants
The preparation method of strain are as follows: take the potting host plant of 8~10 leaf phases, arrange removal bottom old leaf and only retain 4~5 exhibitions completely
After opening leaf, Bemisia tabaci receptacle is moved into, the strain of Bemisia tabaci worm sources is subsequently introduced and worm host plant waiting interval puts and carries out connecing worm
Raising, shaking worm sources strain makes Bemisia tabaci adults diffusion fly on worm host plant waiting and (be shaken every day 1 time), when host plant
Bemisia tabaci connects worm amount and reaches 10~15 adult/cm on blade2When, worm sources strain is removed, it is spare after continuing raising 1 week.Preferably,
The selected insect for raising ladybug is Bemisia tabaci in the present invention, and the host plant of Bemisia tabaci is eggplant.
Therefore, another object of the present invention is to obtain Serangium japonicum pupa by the above method, and pass through the party
The Serangium japonicum pupa that method obtains, the purposes in the artificial large-scale breeding of Serangium japonicum.
I.e. the present invention provides a kind of artificial large-scale breeding method of Serangium japonicum, the method is to have used as above
State the Serangium japonicum pupa of method collection.
Preferably, the artificial large-scale breeding method of Serangium japonicum of the present invention, including obtain the above method
Serangium japonicum pupa, concentration are placed in grandis adults rearging cage, after 3~4 days when discovery has grandis adults emergence, are raising
In cage place infection Bemisia tabaci host plant, the adult newly to sprout wings can on host's Plant Leaf feeding, mating, through this feed 7
After~10 days, most of adult has mated and has spent preoviposition period.
Preferably, in the artificial large-scale breeding method of Serangium japonicum of the present invention, the grandis adults rearging cage
To be placed in insectary, the environment temperature of the insectary is 25~27 DEG C, relative humidity is RH 60%~65%, photoperiod
For L14h/D10h.
Preferably, the insect for raising ladybug selected in the present invention is Bemisia tabaci, and the host plant of Bemisia tabaci is
Eggplant.
From the foregoing, it will be observed that the collection method of Serangium japonicum pupa of the invention and the large-scale breeding side of Serangium japonicum
Method at least has the advantage that
The present invention makes full use of the habit of pupating of Serangium japonicum, using the convenient single face corrugated board system of material source
It pupates circle, production method is easy, and it is easy that pupa collects operating process, can not only effectively collect ladybug pupa, realize the concentration of pupa
It collects, the advantages of concentrating emergence, mating and oviposition and the raising of the concentration of larval phase of adult, and reusable, overcomes
Usual Serangium japonicum mature larva like to be clustered in old leaf blade back, plastic tub alms bowl edge groove or in insectary other
Hidden corner is pupated, more dispersed, not the difficult point of easy-to-search and collection, is able to satisfy the demand of natural enemy ladybug large-scale breeding,
Obtain the neat each worm state ladybug of development.
Host range is wide for Bemisia tabaci, field occur extensively, as long as and reproductive capacity is strong, the generation cycle is short, it is indoor cyclically
Substitute host plant, can keep higher population quantity, the present invention using Bemisia tabaci as the prey for breeding Serangium japonicum,
Method for breeding is easy, time saving and energy saving, easily operated.Eggplant is the selection of Bemisia tabaci preference and one of most suitable host plant, tool
Have the characteristics that cultivation period is short, resistance is strong, leaf area is big, the present invention is using eggplant as the host plant of breeding Bemisia tabaci, energy
Enough meet efficient, the needs quickly bred of prey Bemisia tabaci.Therefore using eggplant as the host plant of breeding Bemisia tabaci, with cigarette
Prey of the aleyrodid as raising Serangium japonicum, Serangium japonicum are raised in the present inventive method, and every plant under normal circumstances
It can get 60~80 grandis adults on eggplant plant, each pair of Serangium japonicum adult can breed 100~150 offsprings of acquisition
Adult realizes Serangium japonicum and efficiently, quickly, in high volume breeds for the prior art.Therefore the present invention has section
Learn reasonable, simple, convenient and practical advantage.
Detailed description of the invention
Fig. 1 is the schematic diagram of the strip single face corrugated board of the embodiment of the present invention 1；
Fig. 2 is the photo of the strip single face corrugated board of the embodiment of the present invention 1；
Fig. 3 is the photo of circle of pupating made of the single face corrugated board of the embodiment of the present invention 1.
Specific embodiment
A kind of collection side of Serangium japonicum pupa that operation is easy and efficient is applicable in is provided in an embodiment of the present invention
Method, comprising the following steps:
A. it obtains Ladybird egg: the host plant for infecting Bemisia tabaci being moved into grandis adults rearging cage, collects egg-laying season ladybug
Female adult pest is in the produced Ladybird egg in the host's blade back side；
B. raise children phase ladybug: step a, which is produced, has the host plant of Ladybird egg to move into the raising of ladybug receptacle；
C. it collects ladybug pupa: when ladybug children phase (ovum phase+larval phase) grows to the 12nd day, being put in host's plant basal part of stem
Set circle of pupating；When children phase (ovum phase+larval phase) grows to the 14th~15 day, the circle that will pupate is taken out, and is placed in grandis adults raising
In cage.
In another embodiment of the present invention, replaced daily in the ladybug female adult pest egg-laying season of the step a infected with
The host plant of Bemisia tabaci, it is sustainable to obtain the relatively uniform Ladybird egg of development condition.
In another embodiment of the present invention, the feeding time of the children phase ladybug of the step b is 14~15d.
In another embodiment of the present invention, the circle of pupating of the step c of single face corrugated board by being made.Such as figure
Shown in 1, which is the corrugated board being bonded by two layers of cardboard and one layer of corrugated paper.
In another embodiment of the present invention, as shown in Fig. 2, it is described pupate circle for using long 45~50cm, it is wide by 6~
The single face corrugated board of the strip of 7cm is made；In yet another embodiment of the present invention, it is described pupate circle for using it is long by 45~
The single face corrugated board of the strip of 50cm, wide 6~7cm is surround along host's plant basal part of stem, and is fixed into cylindrical pupate
Circle；It is described to pupate circle to surround host plant 3 using made of single face corrugated board in yet another embodiment of the present invention
~4 circles, 6~7cm of height, 3~4cm of inner ring diameter, outer ring 6~8cm of diameter cylinder pupate circle；In another implementation of the invention
In example, the circle of pupating is placed in host's plant basin alms bowl on Culture media, first in host's plant basin before circle is pupated in placement
One layer of preservative film is padded in alms bowl on Culture media.
In another embodiment of the present invention, the preparation method of the host plant of the infection Bemisia tabaci are as follows: take 8~10
The potting host plant of piece leaf phase moves into Bemisia tabaci raising after arrangement removal bottom old leaf only retains 4~5 fully expanded leaves
Room, is subsequently introduced the strain of Bemisia tabaci worm sources and worm host plant waiting interval puts and connect worm raising, and shaking worm sources strain makes tobacco powder
The diffusion of lice adult flies on worm host plant waiting and (is shaken every day 1 time), when Bemisia tabaci connects worm amount and reaches on host's plant leaf
To 10~15 adult/cm2When, worm sources strain is removed, it is spare after continuing raising 1 week；Preferably, the host plant is eggplant.
Preferably, the insect for raising ladybug selected in the embodiment of the present invention is Bemisia tabaci, the host of Bemisia tabaci
Plant is eggplant.
In one embodiment of the invention, a kind of artificial large-scale breeding method of Serangium japonicum is provided, it is described
Method is to have used the Serangium japonicum pupa collected such as the above method.
In another embodiment of the present invention, the artificial large-scale breeding method of Serangium japonicum of the present invention, packet
The Serangium japonicum pupa for obtaining the above method is included, concentration is placed in grandis adults rearging cage, when discovery has wooden dipper after 3~4 days
When worm adult eclosion, the host plant of infection Bemisia tabaci is placed in rearging cage, the adult newly to sprout wings can be on host's Plant Leaf
Feeding, mating, after this feeds 7~10 days, most of adult has mated and has spent preoviposition period.
In another embodiment of the present invention, in the artificial large-scale breeding method of Serangium japonicum of the present invention,
The grandis adults rearging cage is to be placed in insectary, and the environment temperature of the insectary is 25~27 DEG C, relative humidity RH
60%~65%, photoperiod L14h/D10h.
Preferably, the insect for raising ladybug selected in the embodiment of the present invention is Bemisia tabaci, the host of Bemisia tabaci
Plant is eggplant.
Further technical solution of the present invention is illustrated below by way of specific embodiment, it should be understood that be below only this hair
Bright exemplary illustration, is not intended to restrict the invention scope of protection of the claims.
Embodiment 1: the collection method of Serangium japonicum pupa
1, step a Ladybird egg obtains: the eggplant plant for having infected Bemisia tabaci is moved into grandis adults rearging cage (using nylon
The conventional dependent insect cage of gauze production, wide 40cm × depth 45cm × high 50cm), egg-laying season ladybug female adult pest can be automatically in eggplant leaf
Back side oviposition；Eggplant plant of the replacement daily infected with Bemisia tabaci, i.e., the relatively uniform Ladybird egg of sustainable acquisition development condition.
2, step b ladybug children phase is raised: step a, which is produced, has the potting eggplant plant of Ladybird egg to move into the raising of ladybug receptacle
14~15d；
3, ladybug pupa is collected: when ladybug children phase (ovum phase+larval phase) grows to the 12nd day, being put in eggplant plant basal part of stem
Set circle of pupating；When children phase (ovum phase+larval phase) grows to the 14th~15 day, the circle that will pupate is taken out, and is placed in grandis adults raising
In cage.
Circle of wherein pupating makes and its use technology:
It is made of single face corrugated board, single face corrugated board is cut into the strip of long 45~50cm, wide 6~7cm first
Then above-mentioned strip corrugated board is enclosed along potting eggplant plant basal part of stem around 3~4, and uses pin by shape (referring to fig. 2)
Be fixed into 6~7cm of height, 3~4cm of inner ring diameter, outer ring 6~8cm of diameter cylinder pupate circle, be placed in Culture media in basin alms bowl
On, circle water suction dampness of pupating in order to prevent first pads one layer of preservative film before circle is pupated in placement in basin alms bowl on Culture media.
The acquisition of the infection Bemisia tabaci eggplant plant: taking the potting eggplant plant of 8~10 leaf phases, arranges removal bottom
After portion's old leaf only retains 4~5 fully expanded leaves, Bemisia tabaci receptacle is moved into, the strain of Bemisia tabaci worm sources and worm waiting is subsequently introduced
Eggplant strain interval, which is put, connect worm raising, and shaking worm sources strain flies Bemisia tabaci adults diffusion on worm eggplant plant waiting
(being shaken every day 1 time), when Bemisia tabaci connects worm amount and reaches 10~15 adult/cm on eggplant plant leaf2When, worm sources strain is removed,
It is spare after continuing raising 1 week.
Embodiment 2: circle of pupating evaluates the collecting effect of Serangium japonicum pupa
Serangium japonicum mature larva like to be clustered in old leaf blade back, plastic tub alms bowl edge groove or in insectary its
He is hidden, and corner is pupated, more dispersed, it is difficult to which therefore how centralized collection improves the receipts of ladybug pupa in extensive raising
Collect efficiency, is of great significance to reproductive efficiency is improved.
1, ladybug is made as material using single face corrugated board to pupate circle.
Firstly, single face corrugated board is cut into strip corrugated board (such as Fig. 2), length 45cm, width separately design for
3.0cm, 4.0cm, 5.0cm, 6.0cm, 7.0cm then, will be above-mentioned when ladybug children phase (ovum+larval phase) grows to the 12nd day
Strip corrugated board is enclosed along potting eggplant plant basal part of stem around 3, and is fixed into cylinder with pin and is pupated circle (as schemed
3), corresponding circle height of pupating is respectively 3.0cm, 4.0cm, 5.0cm, 6.0cm, 7.0cm (A, B, C, D, E of corresponding diagram 3).For
Prevent from pupating circle water suction dampness, place pupate circle before first one layer of the Culture media upper berth in plastic tub alms bowl preservative film.
When children phase (ovum+larval phase) grows to the 14th~15 day, the circle that will pupate is taken out, and counts ladybug on various strip corrugated boards
The quantity of pupa.In view of small part ladybug mature larva can pupate at the eggplant leaf back side, this part ladybug pupa be easy to find and
It collects, therefore, pupa collection rate calculates as follows:
Pupa collection rate (%)=and (pupate and enclose pupa quantity+eggplant leaf pupa quantity)/4 instar larvae quantity × 100 before pupating
2, test result
Table 1 the results show that the circle of pupating of different height to the collecting effect significant difference of ladybug pupa, wherein height 6.0cm
92.14% and 94.98% is up to respectively to the collection rate of ladybug pupa with 7.0cm circle of pupating, be all remarkably higher than 3.0cm, 4.0cm,
5.0cm height is pupated circle.Therefore, circle of pupating is made using the strip single face corrugated board of long 45cm, wide 6.0~7.0cm, it can
Realize the efficient collection of ladybug pupa, it is easy to operate.
Table 1, which pupates to enclose, evaluates the collecting effect of Serangium japonicum pupa
Embodiment 3: the artificial large-scale breeding method of Serangium japonicum
The large scale artificial breeding of Serangium japonicum is the host plant using eggplant as breeding Bemisia tabaci, is made with Bemisia tabaci
For the prey for raising Serangium japonicum, it is related to eggplant plant cultivating, the raising of prey Bemisia tabaci, the collection of Ladybird egg, ladybug children
Several processes such as the raising of worm phase, the collection of ladybug pupa and grandis adults raising.
1, eggplant plant cultivating
(1) in market, purchase is used for the Culture media of nursery, then Nutrition Soil is packed into the long 58cm × wide 30cm got ready
Seedlings nursing plate in it is spare；
(2) it using eggplant as the host plant of breeding Bemisia tabaci, in the eggplant seed of market purchase easy infection Bemisia tabaci, broadcasts
It is impregnated 30 minutes before kind with the carbendazim solution of effective component 0.1%, then, seed is placed in wet gauze and is wrapped, be placed on
Vernalization under conditions of 28~30 DEG C, when have 50% radicle it is exposed when can sow；
(3) type of seeding is 3~4g/m of sowing quantity by eggplant seed uniform broadcasting in seedlings nursing plate2, after planting with sieving
Nutrition Soil 1~2cm of earthing waters in time, is sufficiently humidified so as to Nutrition Soil；
(4) seedling culture frame is built in seedling raising greenhouse, 2 layers of frame point, there are the channels for facilitating management between frame, nursery after planting
Disk is placed on seedling culture frame；
(5) water and fertilizer management was observed every 2~3 days and is watered in time when Nutrition Soil is dry；
(6) with pincet carry out opportune thinning after eggplant emergence, when observing that eggplant seedling growth and development state is not good enough with
Compound fertilizer or the low ratio of urea are watered to pour and apply；
(7) it is transplanted when eggplant seedling grows into 3~4 leaves, when transplanting loads base in diameter 18cm plastic tub alms bowl
Matter Nutrition Soil after being watered with water, is implanted into plastic tub alms bowl from seedlings nursing plate lifting with small medicine spoon, every basin alms bowl transplants 1 plant, moderately adds after slow seedling
Strong water and fertilizer management；
(8) other pests such as red spider, thrips, aphid to be removed in time in seedling raising process, avoid bringing into Bemisia tabaci and
Ladybug receptacle.
2, the raising of prey Bemisia tabaci
(1) when eggplant plant strain growth to 8~10 leaves, removal bottom old leaf retains 4~5 fully expanded leaves, moves into
The biggish worm sources strain of density of Bemisia tabaci adults is subsequently introduced in Bemisia tabaci receptacle；
(2) worm sources strain and worm eggplant plant waiting connect worm raising apart from one another by putting, shaking worm sources strain makes tobacco powder
The diffusion of lice adult is flown on worm eggplant plant waiting；When on eggplant plant leaf Bemisia tabaci connect worm amount reach 10~15 at
Worm/cm2When, remove worm sources strain.
(3) feeding worm frame has been built in receptacle, each feeding three layers, 55~60cm every layer high, depth 60cm of worm frame point, between frame
There are the channels for facilitating management；
(4) it worm eggplant strain basin alms bowl will have been connect has moved on feeding worm frame and managed concentratedly, moved into before Serangium japonicum receptacle
First raised 1 week in Bemisia tabaci receptacle；
(5) the eggplant plant of Bemisia tabaci is infected in addition to for raising Serangium japonicum, also needing to stay a part as newly
Worm sources strain is bred for Bemisia tabaci, to maintain Bemisia tabaci quantity constant or increase；
(6) insectary control condition is 25~27 DEG C of temperature, humidity 60%~65%, photoperiod L14/D10.
3, the mass propagation of Serangium japonicum
(1) Ladybird egg obtains: the eggplant strain for having infected Bemisia tabaci is moved into grandis adults rearging cage (using nylon gauze system
The conventional dependent insect cage of work, wide 40cm × depth 45cm × high 50cm), egg-laying season ladybug female adult pest can produce at the eggplant leaf back side automatically
Ovum；Eggplant plant of the replacement daily infected with Bemisia tabaci, i.e., the relatively uniform Ladybird egg of sustainable acquisition；
(2) ladybug children phase is raised: will be produced the potting eggplant strain for having Ladybird egg and is moved into ladybug receptacle, maintains plant raw at this time
It is long good, so that Bemisia tabaci keeps enough population quantities, sufficient prey is provided for ladybug.Feeding worm has been built in receptacle
Frame, each feeding three layers, 55~60cm every layer high, depth 60cm of worm frame point, there are the channel for facilitating management, Japanese nose angle wooden dippers between frame
Worm is raised on a large scale to carry out on feeding worm frame.
(3) ladybug pupa is collected: when ladybug children phase (ovum+larval phase) grows to the 12nd day, being placed in eggplant strain basal part of stem
It pupates circle；When children phase (ovum+larval phase) grows to the 14th~15 day, the circle that will pupate is taken out, while being planted with the careful picking of writing brush
The small part pupa of strain middle and lower part vacuum side of blade.
(4) grandis adults are raised: the same batch ladybug pupa concentration of collection being placed in grandis adults rearging cage, after 3~4 days
When finding that grandis adults start to sprout wings, the eggplant plant for having infected Bemisia tabaci, the adult meeting newly sprouted wings are placed in rearging cage
Automatically feeding, mating on eggplant leaf, after this raising 7~10 days, most of adult has mated and has spent preoviposition period.
(5) insectary control condition is 25~27 DEG C of temperature, humidity 60%~65%, photoperiod L14/D10.
Eggplant plant is 8~10 leaf periods, and reservation 4~5 is fully deployed blade after removing bottom old leaf.Eggplant plant
It is 10~15 adult/cm that upper Bemisia tabaci, which connects worm amount,2, Serangium japonicum Adult worms producting eggs 1d is introduced after raising 1 week.By one
The raising of generation cycle can raise 60~80 grandis adults of acquisition, each pair of Serangium japonicum adult on every plant of eggplant plant
100~150 offspring adults of acquisition can be bred.
Circle of pupating is made of common single face corrugated board, and single face corrugated board is cut into long 45~50cm, wide by 6 first
The strip of~7cm then along eggplant strain basal part of stem around 3~4 circles, and is fixed into height 6~7cm, inner ring diameter 3 with pin
~4cm, outer ring 6~8cm of diameter cylinder pupate circle, be placed in basin alms bowl on Culture media, pupate in order to prevent circle water suction by
Tide first pads one layer of preservative film before circle is pupated in placement in basin alms bowl on Culture media.Serangium japonicum mature larva
It can creep down along eggplant strain stem when pupa, and in piercing automatically and pupating and enclose, therefore, circle of pupating provides for Serangium japonicum
One is concentrated the place pupated, and realizes the efficient collection of ladybug pupa；Circle of pupating with pupa is placed in imago breeding room just
The adult of emergence can be obtained, a basin is placed in receptacle infected with the eggplant plant of Bemisia tabaci, new emergence grandis adults can be certainly
It moves in the feeding of the eggplant leaf back side, mating and oviposition；Replace the eggplant plant infected with Bemisia tabaci daily in the ladybug egg-laying season,
It is i.e. sustainable to obtain relatively uniform Ladybird egg.
Embodiment 4: selectivity and adaptability of the Bemisia tabaci to host plant
1, test method
Choice tests: pachyrhizus, cotton, eggplant, 4 kinds of cucumber are beaten for the examination fresh blade of host plant respectively with punch
At the sequin of diameter 35mm, face of blade is layered on the training with cowling of diameter 110mm along culture dish inner wall edge downward
It supports in ware (agar for the 12g/L of falling people in advance in culture dish), random alignment.Each culture dish access 100~150 pairs of Bemisia tabacis at
It is inverted after worm, indoor light keeps uniform.After connecing worm for 24 hours, the Bemisia tabaci adults in each host plant blades of investigation records are fallen
Borer population and ovum amount, are repeated 10 times.
Adaptability test: taking 20 mixing age in days Bemisia tabaci female adult pests, is fixed on blade cage each for examination host plant plant
Strain vacuum side of blade removes blade cage and rejects the Bemisia tabaci adults on blade after laying eggs for 24 hours.Move to 27 DEG C of temperature, relatively wet
It spends in the incubator of 65%, photoperiod L14/D10, day by day observation statistics ovum and nymph survival rate, development duration and adult eclosion
Rate, every processing are repeated 4 times.
2, test result
Host selection test result (table 2) display, after connecing worm for 24 hours, Bemisia tabaci adults are on eggplant, the small roundleaf of cucumber
It falls worm amount (respectively 85.50 and 84.10) and is significantly higher than on sweet potato and the small roundleaf of cotton and fall worm amount (respectively 30.20
Head and 46.0)；Egg laying amount (respectively 882.80 and 681.50) of the Bemisia tabaci adults on eggplant and the small roundleaf of cucumber
Egg laying amount (respectively 143.30 and 239.30) also significantly greater than on the small roundleaf of sweet potato and cotton, shows Bemisia tabaci to not
There is apparent feeding and oviposition selectivity with host plant, adult obviously has a liking for eggplant and cucumber.
Host's adaptability test result (table 2) display the generation development duration of Bemisia tabaci and is deposited under 27 DEG C of indoor conditions
Motility rate significant difference between different host plants, wherein generation development duration longest (reaching 19.86d) on the cotton, significantly
It is longer than the generation development duration (respectively 18.08d, 17.53d and 17.92d) on eggplant, cucumber and sweet potato；On the cotton
Generation survival rate is minimum (only 56.89%), and the generation survival rate on eggplant, cucumber may be up to 81.75% and 87.28%,
The generation survival rate being significantly higher than on cotton and sweet potato.
In summary test result shows compared with sweet potato and cotton, and it is obvious that eggplant and cucumber are not only Bemisia tabaci adults
Preference selects the host plant of (feeding and oviposition), and becomes apparent from suitable Bemisia tabaci offspring growth and development, it is contemplated that cucumber
Belong to annual climber herbaceous plant, is not suitable for indoor culture, and eggplant belongs to semishrub plant, is easy to indoor culture, therefore recognize
For the host plant using eggplant as breeding Bemisia tabaci is more suitable for.
Selectivity and adaptability of 2 Bemisia tabaci of table to different host plants
Embodiment 5: selectivity and adaptability of the Serangium japonicum to host plant
1, test method
Choice tests method: it selects eggplant, pachyrhizus, cotton, cucumber leaves as test material, certain amount is taken to mix
Age in days Bemisia tabaci female adult pest is individually fixed in above-mentioned for trying host plant without worm plant leaf back with the blade cage of diameter 30mm
Face, lay eggs 48h after (ovum amount is up to 800 or more), remove blade cage and reject the Bemisia tabaci adults on blade, distinguished with punch
Each sequin for breaking into diameter 33cm for the examination fresh blade of host plant with tobacco powder nit is spare.It (1) will be with tobacco powder nit
4 kinds of host plant roundlet face of blade be layered in diameter 110cm culture dish along culture dish inner wall edge downward, each culture
Every kind of host plant puts 1 in ware, and random alignment, every ware puts into egg-laying season Serangium japonicum female adult pest 1, then will training
Feeding ware is inverted in the incubator of photoperiod L14/D10,27 DEG C of temperature, relative humidity 65%, for 24 hours after each confession is checked under anatomical lens
The prey tobacco powder nit amount and ladybug egg laying amount on host plant sequin are tried, each processing is repeated 10 times.(2) only cotton roundlet
On piece has tobacco powder nit, other 3 kinds of host plant sequins are without tobacco powder nit, the same method of other steps (1), for 24 hours
Each ladybug egg laying amount on examination host plant sequin is checked afterwards, and each processing is at least repeated 10 times.
Adaptability test method: (1) discharge observation is preyed on: respectively will be with each confession of tobacco powder nit (ovum amount is up to 800 or more)
Host plant sequin is tried, face down is layered in the culture dish of diameter 35mm, and every ware puts into egg-laying season ladybug female adult pest 1,
Then culture dish is inverted in the incubator of photoperiod L14/D10,27 DEG C of temperature, relative humidity 65%, for 24 hours after under anatomical lens
Prey tobacco powder nit amount, every processing are at least repeated 10 times.(2) reproductive capacity is observed: respectively will (ovum amount is up to 800 with tobacco powder nit
Or more) it is each for trying host plant sequin, face down is layered in the culture dish of diameter 35mm, plumage at the beginning of every ware puts into 1 pair
Change grandis adults, then culture dish is inverted in the incubator of photoperiod L14/D10,27 DEG C of temperature, relative humidity 65%, for 24 hours
Adult is moved on on the host plant sequin of the same race with same food afterwards, is observed and recorded under anatomical lens and replaces blade
On ladybug egg laying amount, in this way per for 24 hours shift 1 time, until adult death, it is every processing at least observe 10 pairs of adults.(3) growth hair
It educates and survival rate is observed: will respectively handle the produced ovum of grandis adults in egg laying amount observation experiment, move to 27 DEG C of temperature, relative humidity
It is cultivated under conditions of 65% and photoperiod L14/D10, after egg hatching, newly hatched larvae is moved into band sufficient amount Bemisia tabaci adults
It is each for observing growth and development, the survival condition of each instar larvae day by day in examination host plant blades with ovum, count each age children
The development duration and survival rate of worm.Every processing starting worm amount is 100 ovum.
2, test result
Table 3 the results show that under the comparable alternative condition of prey quantity, Serangium japonicum eggplant, cucumber, cotton,
The selection rate of looking for food of pachyrhizus blade is respectively 73.67%, 15.45%, 7.17 and 3.71%, the amount of looking for food is respectively 401.39,
108.94,58.44 and 20.50；Oviposition selection rate is respectively 72.75%, 18.55%, 0 and 8.70%, and egg laying amount is respectively
9.22,3.17,0 and 1.67, difference reaches the level of signifiance.In selectivity experiment of the only cotton leaf with aleyrodid ovum, Japan
Serangium japonicum female adult pest is preyed on the cotton leaf with prey, but shows the big fecund of ovum on the eggplant leaf without prey
Serangium japonicum is to the specific significant oviposition selectivity of eggplant leaf.
Oviposition selection of 3 Serangium japonicum of table to different host plants
Table 4 is the results show that in non-selection experiment, and Serangium japonicum is to Bemisia tabaci on eggplant, cucumber, cotton and sweet potato
The predatory number of ovum is respectively 588.50,507.47,488.42 and 502.50, and difference is not significant.Serangium japonicum eggplant,
Average every female egg laying amount on cucumber, cotton and sweet potato is respectively 479.80,454.60,383.20 and 433.10, difference
It is not significant；Serangium japonicum on eggplant by ovum-at average development duration be 16.0d, with development duration on the cotton
Quite, the development duration substantially less than on cucumber and sweet potato；Serangium japonicum completes the generation developed from generation to generation on eggplant
Survival rate be 37.93%, on cucumber, sweet potato generation survival rate (respectively 35.56% and 31.48%) quite, significantly
Higher than generation survival rate 20.58% on the cotton.
In summary test result shows that compared with sweet potato, cotton, cucumber, eggplant is not only Serangium japonicum adult
The Bemisia tabaci host plant that obvious preference is looked for food with oviposition selection, and obviously it is suitble to ladybug offspring growth and development, therefore recognize
For breeding Bemisia tabaci to indoor raising Serangium japonicum with eggplant is more suitable for.
Adaptability of 4 host plant of table to Serangium japonicum
Embodiment 6: the worm amount that connects when raising Bemisia tabaci using eggplant selects
It breeds Bemisia tabaci using eggplant as host plant to carry out during raising Serangium japonicum on a large scale, eggplant is planted
Bemisia tabaci quantity directly affects the reproductive effect of Serangium japonicum in strain.Bemisia tabaci quantity is too small to will cause Japan
Serangium japonicum prey lazy weight, Bemisia tabaci quantity is excessive, it is aggrieved excessively to may cause host plant, and in Japanese knife
Angle ladybug not yet completes to occur as soon as blade before developing from generation to generation and wilt to fall off, and influences Serangium japonicum and normally breeds.Therefore, in benefit
Use eggplant plant as host plant raising Bemisia tabaci for inquiring into cigarette on host plant in large-scale breeding Serangium japonicum
Aleyrodid connects worm amount, is of great significance to reproductive efficiency is improved.
Test method: test≤5 She Zhi/cm2, 6~10/cm2, 11~15/cm2 and 16~20/cm24
Different Bemisia tabacis connect the processing of worm amount, and connecing worm method is to take the potting host plant of 8~10 leaf phases, arrange removal bottom old leaf only
After retaining 4~5 fully expanded leaves, Bemisia tabaci receptacle is moved into, the strain of Bemisia tabaci worm sources and worm brinjal plant waiting is subsequently introduced
Interval, which is put, connect worm raising, and shaking worm sources strain makes Bemisia tabaci adults diffusion fly and (shake daily on worm eggplant plant waiting
Dynamic 1 time), when Bemisia tabaci connects worm amount and reaches each processing and connect worm amount on eggplant plant leaf, worm sources strain is removed, raising 1 week is continued
Life food as raising Serangium japonicum is provided afterwards, observes and records eggplant plant situation and ladybug growth and development feelings day by day
Condition.
Test result: table 5 the results show that Bemisia tabaci connect worm amount to Serangium japonicum breeding be affected, when eggplant plant
Bemisia tabaci meets Liang≤5 Chong/cm in strain2When, eggplant plant energy normal growth, but Bemisia tabaci quantity is lower on plant,
Prey wretched insufficiency, it is difficult to meet Serangium japonicum growth and development needs, kill each other serious；When Bemisia tabaci on eggplant plant
Connecing worm amount is 6~10/cm2When, eggplant plant energy normal growth, but Bemisia tabaci quantity is slightly lower on plant part, hunts
Object is insufficient, and there are a degree of autotomy phenomenons；It is 11~15/cm when Bemisia tabaci connects worm amount on eggplant plant2When, eggplant is planted
The equal normal growth of strain, Bemisia tabaci quantity is higher on plant, and prey is sufficient, and ladybug growth and development is normal；When on eggplant plant
It is 16~20/cm that Bemisia tabaci, which connects worm amount,2When, Bemisia tabaci quantity is too high on plant part, excessively causes harm, leads to eggplant plant
Can not normal growth, Serangium japonicum not yet complete from generation to generation develop before occur as soon as blade wilting fall off.In summary as a result,
Think 10~15/cm of selection2Worm amount is connect to the artificial biological control of Serangium japonicum as Bemisia tabaci on eggplant plant
Effect is best.
5 Bemisia tabaci of table connects the influence that worm amount completes generation developmental state to brinjal plant growth, ladybug
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (8)
1. a kind of collection method of Serangium japonicum pupa, comprising the following steps:
A. obtain Ladybird egg: will infect Bemisia tabaci host plant move into grandis adults rearging cage, collect egg-laying season ladybug it is female at
Worm is in the produced Ladybird egg in the host's blade back side；
B. raise children phase ladybug: step a, which is produced, has the host plant of Ladybird egg to move into the raising of ladybug receptacle；
C. it collects ladybug pupa: when ladybug children phase grows to the 12nd day, placing circle of pupating in host's plant basal part of stem；When children phase is raw
At long to the 14th~15 day, the circle that will pupate is taken out, and is placed in grandis adults rearging cage；
The ladybug children phase is the sum of ovum phase and larval phase；
The circle of pupating of the step c of single face corrugated board by being made；
The shape of the single face corrugated board is strip；The length of the single face corrugated board is 45~50cm；The single side
The width of corrugated board is 6~7cm；
The single face corrugated board is surround along host's plant basal part of stem, and is fixed into cylindrical circle of pupating；
The single face corrugated board is around the circle of host plant 3~4, height 6~7cm, 3~4cm of inner ring diameter, outer ring 6~8cm of diameter；
The circle of pupating is placed in host's plant basin alms bowl on Culture media, first in host's plant basin alms bowl before circle is pupated in placement
One layer of preservative film is padded on interior Culture media.
2. the collection method of Serangium japonicum pupa according to claim 1, it is characterised in that: in the wooden dipper of the step a
In the worm female adult pest egg-laying season, the host plant infected with Bemisia tabaci is replaced daily, and sustainable acquisition development condition is relatively uniform
Ladybird egg.
3. the collection method of Serangium japonicum pupa according to claim 1, it is characterised in that: the children phase of the step b
The feeding time of ladybug is 14~15d.
4. according to the collection method of Serangium japonicum pupa described in claim 1, it is characterised in that: the infection Bemisia tabaci is posted
The preparation method of main plant are as follows: take the potting host plant of 8~10 leaf phases, arrange removal bottom old leaf only retain 4~5 it is complete
After full expansion leaf, Bemisia tabaci receptacle is moved into, the strain of Bemisia tabaci worm sources is subsequently introduced and progress is put at worm host plant waiting interval
Worm raising is connect, shaking worm sources strain flies Bemisia tabaci adults diffusion on worm host plant waiting, when cigarette on host's plant leaf
Aleyrodid connects worm amount and reaches 10~15 adult/cm2When, worm sources strain is removed, it is spare after continuing raising 1 week；
The host plant is eggplant；The number of the shake is 1 time.
5. a kind of artificial large-scale breeding method of Serangium japonicum, which is characterized in that the method is that right such as has been used to want
The Serangium japonicum pupa for asking 1-4 any one the method to collect.
6. according to the method described in claim 5, it is characterized in that, the artificial large-scale breeding of the Serangium japonicum further includes
The Serangium japonicum pupa that claim 1-4 any one the method is collected raises the step of grandis adults.
7. according to the method described in claim 6, it is characterized in that, will to be wanted according to right the step of the raising grandis adults
The Serangium japonicum pupa for asking method described in 1~4 any one to obtain, concentration are placed in grandis adults rearging cage, and 3~4 days
Afterwards when discovery has grandis adults emergence, the host plant of infection Bemisia tabaci is placed in rearging cage, the adult newly to sprout wings can be
Feeding, mating on host's Plant Leaf, after this raising 7~10 days, most of adult has mated and has spent preoviposition period.
8. the method according to the description of claim 7 is characterized in that the grandis adults rearging cage be placed in insectary, institute
The environment temperature for stating insectary is 25~27 DEG C, relative humidity is RH 60%~65%, photoperiod L14h/D10h.
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