CN113180011B - Large-scale breeding method for Anagrus nilaparvatae - Google Patents
Large-scale breeding method for Anagrus nilaparvatae Download PDFInfo
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- CN113180011B CN113180011B CN202110495251.1A CN202110495251A CN113180011B CN 113180011 B CN113180011 B CN 113180011B CN 202110495251 A CN202110495251 A CN 202110495251A CN 113180011 B CN113180011 B CN 113180011B
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- 238000009395 breeding Methods 0.000 title claims abstract description 22
- 241000584992 Anagrus Species 0.000 title claims description 15
- 241000209094 Oryza Species 0.000 claims abstract description 242
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 242
- 235000009566 rice Nutrition 0.000 claims abstract description 242
- 241001556089 Nilaparvata lugens Species 0.000 claims abstract description 140
- 241001498622 Cixius wagneri Species 0.000 claims abstract description 62
- 235000013601 eggs Nutrition 0.000 claims abstract description 58
- 241000257303 Hymenoptera Species 0.000 claims abstract description 56
- 238000011081 inoculation Methods 0.000 claims abstract description 42
- 238000009341 apiculture Methods 0.000 claims abstract description 23
- 241000256856 Vespidae Species 0.000 claims abstract description 21
- 241001466042 Fulgoromorpha Species 0.000 claims abstract description 19
- 241001674048 Phthiraptera Species 0.000 claims abstract description 18
- 241000238631 Hexapoda Species 0.000 claims abstract description 17
- 230000019617 pupation Effects 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 14
- 238000005286 illumination Methods 0.000 claims description 28
- 238000004519 manufacturing process Methods 0.000 claims description 16
- 238000009331 sowing Methods 0.000 claims description 13
- 230000001488 breeding effect Effects 0.000 claims description 11
- 241000607479 Yersinia pestis Species 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 230000007613 environmental effect Effects 0.000 claims description 9
- 230000000384 rearing effect Effects 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000004744 fabric Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 210000003746 feather Anatomy 0.000 claims description 5
- 241001414989 Thysanoptera Species 0.000 claims description 4
- 230000035784 germination Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000006096 absorbing agent Substances 0.000 claims description 2
- 238000005057 refrigeration Methods 0.000 claims description 2
- 238000003860 storage Methods 0.000 abstract description 6
- 238000010924 continuous production Methods 0.000 abstract description 4
- 238000010923 batch production Methods 0.000 abstract description 3
- 241000256618 Trichogramma Species 0.000 description 5
- 241000256846 Apis cerana Species 0.000 description 4
- 230000035558 fertility Effects 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 230000001502 supplementing effect Effects 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 2
- 230000027326 copulation Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 241000256836 Apis Species 0.000 description 1
- 241001470016 Laodelphax Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 208000002720 Malnutrition Diseases 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000176086 Sogatella furcifera Species 0.000 description 1
- 241001481304 Vespoidea Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000001071 malnutrition Effects 0.000 description 1
- 235000000824 malnutrition Nutrition 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 238000005580 one pot reaction Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Animal Behavior & Ethology (AREA)
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Abstract
The invention relates to the technical field of insect feeding, and discloses a large-scale breeding method of rice louse ceratosporus sp, which comprises the following steps of cultivating rice seedlings at a seedling stage; step two, transferring the rice seedling pot obtained in the step one into a first insect cage placed in a brown planthopper inoculation and culture room, inoculating brown planthopper adults with a male-female ratio of 1; step three, transferring the rice seedling pot with the brown planthopper eggs obtained in the step two into a beekeeping cage, and inoculating the rice planthopper tassels with a male-female ratio of 1.5-1 into the beekeeping cage, and step four, collecting the bee pupae: placing the rice seedlings with the rice lice tassel wasps in the pre-pupation stage in a refrigerator for cold storage at 4 ℃; collecting adult bees; the method can realize the large-batch and continuous production of the rice planthopper tassel wasps, and can quickly obtain adult rice planthopper wasps to prevent and control the rice planthoppers by refrigerating and storing the rice seedlings with the rice planthopper tassel wasps in the pre-pupation stage.
Description
Technical Field
The invention relates to the technical field of insect feeding, in particular to a large-scale breeding method of small rice-louse thysanodes.
Background
The rice planthopper wasps (Anagrus nilaparvatae Pang et Wang) are widely distributed in main production areas of rice in China, and are important egg-parasitic natural enemies of migratory pests, namely rice planthoppers (the main three types of the rice planthoppers are the brown planthoppers (Nilaparata lugens Stal), sogatella furcifera Horvath and Laodelphax striatusFall, wherein the brown planthoppers are generated and are the heaviest pests, and the Laodelphax striatellus Fall is the second most important pest). However, outbreaks of rice planthoppers have sudden and destructive properties, and the peak period of the rice planthopper wasps in the field is obviously delayed from the large emergence period of the rice planthoppers, so that the rice planthopper wasps (parasitic wasps) cannot effectively control the rice planthoppers during the outbreaks of the rice planthoppers.
Disclosure of Invention
The invention aims to provide a large-scale breeding method for rice planthopper tassel apis cerana, which can realize large-scale and continuous production of the rice planthopper tassel apis cerana and can quickly obtain adult rice planthopper apis cerana apis cerana to prevent and control the rice planthoppers by refrigerating and storing rice seedlings with the rice planthopper tassel apis cerana in a pre-pupation period.
In order to achieve the purpose, the invention provides a large-scale breeding method of the small rice-louse thysanoptera, which comprises the following steps:
step one, sowing rice seeds subjected to germination accelerating treatment until the rice seeds are exposed to the white in a seedling pot, placing the seedling pot after sowing in a clean rice seedling culture room, and culturing until the seedling stage is reached for later use; wherein the environmental conditions of the clean rice seedling culture room are as follows: the temperature is 25-31 ℃, the relative humidity is 70-90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is more than 20000Lux;
step two, transplanting the rice seedling pot obtained in the step one into a first insect cage placed in a brown planthopper inoculation and culture room, inoculating brown planthopper adults with a male-female ratio of 1 to the first insect cage, and marking the inoculation date of the brown planthopper adults; after the brown planthoppers lay eggs, removing and recycling brown planthopper adults from the rice seedlings with brown planthopper eggs, and using the recycled brown planthopper adults to be inoculated onto the rice seedling pots obtained in the first step and fed in a first insect-feeding cage; wherein, the environmental conditions of the brown planthopper inoculation and culture room are as follows: the temperature is 22-28 ℃, the relative humidity is 70-90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is 30000-33000 Lux;
step three, transferring the rice seedling pot with the brown planthopper eggs obtained in the step two into a beekeeping cage placed in a rice planthopper larva inoculation and culture room, inoculating rice planthopper larvae with a female-male ratio of 1.5-1 into the beekeeping cage, marking the inoculation date of the rice planthopper larvae, and regularly adding a nutrient solution for the rice planthopper larvae to eat into the beekeeping cage; the environmental conditions of the inoculation and culture room for the Anagrus nilaparvatae are as follows: the temperature is 29-31 ℃, the relative humidity is 70-90%, the photoperiod is 14h of illumination and 10h of darkness, and the illumination intensity is 30000-33000 Lux;
step four, a, collecting bee pupae: collecting and collecting brown planthopper nymphs on the rice seedlings 2-3 d after the rice seedlings with the brown planthopper eggs are potted with bees, inoculating the brown planthopper nymphs collected from the bee-keeping cages onto the rice seedling pots obtained in the first step, and placing the rice seedling pots in a second insect-keeping cage in a brown planthopper inoculation and culture room for feeding; in the 4 th day after the rice seedlings with brown planthopper eggs are potted and inoculated with the bees, the rice seedlings with the rice planthopper tassel wasps in the pre-pupation stage are placed in a refrigerator at the temperature of 4 ℃ for cold storage; when the feed needs to be used, the refrigerated rice seedlings with the rice planthopper tassel trichogramma in the pre-pupation stage are placed in the field, so that the rice planthopper tassel trichogramma automatically feather into adult bees;
b, adult bee collection: 8-9 d after the rice seedlings with the brown planthopper eggs are potted with the bees, most rice lice tassel bees develop to the cryptogamic period, the other rice lice tassel bees develop to the later period of pupae, the rice lice tassel bees are in the bee emergence period at the moment, the brown planthopper nymphs in the bee rearing cages are recovered and collected before the bees emerge, the brown planthopper nymphs collected from the bee rearing cages are inoculated to the rice seedling pots obtained in the first step, and the rice seedling pots which are inoculated with the brown planthopper nymphs are placed in a second insect rearing cage in a brown planthopper inoculation and cultivation room for rearing; during the bee-out period, covering the bee-keeping cage by black shading cloth, arranging bee-out holes on the black shading cloth, placing transparent tubes for collecting the bees in the bee-out holes, and simultaneously adjusting the photoperiod of the small rice lice tassel inoculating and culturing room to 24h illumination and 0h darkness during the bee-out period; and dividing the collected adult bees into two parts, wherein one part is used for releasing the field control of the rice planthoppers, and the other part is reserved for the production of the rice planthopper thysanoptera of the next batch.
As a preferable scheme of the invention, in the second step, the temperature of the brown planthopper inoculation and culture chamber is 25 ℃, and the illumination intensity in the brown planthopper inoculation and culture chamber is 32000Lux.
As a preferable scheme of the method, in the second step, rice seedlings of 10 days old are transplanted into the first insect-raising cage and inoculated with brown planthopper adults.
As a preferable scheme of the invention, in the second step, after the 3 rd step of inoculation, inverting the rice seedlings with the eggs of the brown planthoppers to enable the adult brown planthoppers to fall off, checking whether the rice seedlings with the eggs of the brown planthoppers have the residual adult brown planthoppers or not, if the residual brown planthoppers exist, removing the adult brown planthoppers through a pest absorber or a glass finger-shaped tube, and collecting and recycling all the adult brown planthoppers; and (3) inoculating the brown planthopper imagoes collected from the rice seedling pot with the brown planthopper eggs on the rice seedling pot obtained in the step one, and placing the rice seedling pot with the brown planthopper imagoes in a first insect feeding cage in a brown planthopper inoculation and culture room to feed and lay eggs.
As a preferable scheme of the invention, in the third step, the ratio of the male and female of the small rice-lice tassel bees which are inoculated to the beekeeping cage is controlled to be 1.
In a preferred embodiment of the invention, in step three, the temperature of the inoculation and culture chamber for the Anagrus nilaparvatae is 30 ℃, and the illumination intensity of the inoculation and culture chamber for the Anagrus nilaparvatae is 32000Lux.
As a preferable scheme of the invention, in the third step, the rice seedling pot with 1-day-old nilaparvata lugens eggs is adopted to be inoculated to the rice planthoppers.
As a preferable scheme of the present invention, in the fourth step, when the nymphs of brown planthoppers in the second insect cages are grown to 4 years old, the rice seedling pots in the second insect cages are replaced with the rice seedling pots obtained in the first step.
As a preferable scheme of the invention, in the step one, firstly, rice seeds are washed clean by clear water, blighted grains and impurities are removed, then the rice seeds are placed in a constant temperature incubator at 35 ℃ and soaked for 24 hours by clear water, the rice seeds are washed again and drained, the rice seeds are covered by a wet towel and are cultured for 24 hours in the constant temperature incubator at 35 ℃, and the rice seeds are in an exposed white state.
As a preferable scheme of the invention, in the step one, after sowing, nutrient solution is added into a seedling raising pot every 12 hours, and the nutrient solution added each time just submerges the rice seeds; normal watering at 4d after sowing.
Compared with the prior art, the large-scale breeding method for the Anagrus nilaparvatae in the embodiment of the invention has the beneficial effects that:
according to the method, the large-batch and continuous production of the rice planthopper wasps can be realized through cultivation of the rice seedlings, production of the rice seedlings with brown planthopper eggs, breeding of the brown planthoppers, breeding of the rice planthopper wasps and preservation of the pupae, the bred brown planthoppers and the recovered rice planthopper wasps are recovered at the same time, the sustainable production of the rice planthopper wasps is realized, and the rice planthopper can be prevented by artificially and timely supplementing the number of the field rice planthopper wasp populations; and the length of the storage time can be determined according to the pest prevention and control requirements by refrigerating and storing the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period, and when the rice planthoppers are harmful rice, the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period can be immediately placed in the field, so that the rice planthopper tassel wasps automatically feather into adult wasps to quickly prevent and control the rice planthoppers.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The embodiment of the invention provides a large-scale breeding method of Anagrus nilaparvatae, which comprises the following steps:
in the first step of the method,
1.1, cleaning rice seeds with clean water, removing blighted grains and impurities, then placing the rice seeds in a constant-temperature incubator at 35 ℃ to be soaked for 24 hours with the clean water, then cleaning again, draining off water, covering the rice seeds with a wet towel, and culturing for 24 hours in the constant-temperature incubator at 35 ℃ to ensure that the rice seeds are subjected to germination accelerating treatment until the rice seeds are in a white exposed state; wherein the rice seeds are temperature-sensitive conventional rice variety Huanghuazhan seeds;
1.2, sowing the rice seeds subjected to germination accelerating treatment until the rice seeds are exposed to the white in seedling pots, and sowing about 1300 rice seeds (the dry mass of the rice seeds is 25 g) in the exposed state in each seedling pot, wherein the rice seeds are uniformly contained in the pots according to the principle of uniform and consistent packing and the elasticity is moderate; the seedling raising pot adopts the following specifications: a white plastic pot with the length of 20cm, the width of 14cm and the height of 5 cm; placing the sowed seedling-cultivating pot in a clean rice seedling cultivating chamber, wherein the environmental conditions of the clean rice seedling cultivating chamber are as follows: the temperature is 28 ℃, the relative humidity is 80%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is more than 20000Lux;
1.3, after sowing, adding nutrient solution into the seedling raising pot every 12 hours, wherein the nutrient solution added each time just submerges the rice seeds, and the root systems of the rice seedlings are loosely combined, so that the nutrient solution of the rice seedlings is prevented from being dispersed when the nutrient solution is added; normal watering at 4d after sowing; the growth height of the rice seedlings on the 10 th day after sowing is 8-9 cm for later use; wherein 972 pots of rice seedlings are produced in each batch, wherein 810 pots of rice plantlets are used for producing the Anagrus nilaparvatae, and the other 162 pots of rice plantlets are used for producing the brown planthoppers;
in the second step, the first step is that,
2.1, transplanting the 10-day-old rice seedling pots obtained in the first step into first insect cages placed in a brown planthopper inoculation and culture room, placing 10 pots in each first insect cage, inoculating brown planthopper adults with a male-female ratio of 1; the environmental conditions of the brown planthopper inoculation and culture room are as follows: the temperature is 25 ℃, the relative humidity is 80%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is 32000Lux; as the brown planthopper likes warmth and wetness, the reproduction of the brown planthopper is influenced if the temperature exceeds a suitable temperature range, and as the egg laying amount of the imagoes is maximum at 24.6 ℃, 25 ℃ is selected as a culture temperature; wherein the area of the brown planthopper inoculation and culture chamber is 18m 2 At least 81 insect cages can be placed;
2.2, after the 3 rd day of inoculation, inverting the rice seedling pot with the brown planthopper eggs to enable the brown planthopper adults to fall off, checking whether the rice seedlings with the brown planthopper eggs have brown planthopper adult residues or not, removing the brown planthopper adults through a pest sucking device or a glass finger tube if the brown planthopper eggs have the brown planthopper adult residues, and collecting and recycling all the brown planthopper adults, so that the rice seedling pot with the 1-day-old brown planthopper eggs is obtained;
2.3, inoculating the brown planthopper adults recovered from the step 2.2 onto the rice seedling pot obtained in the step one, feeding the rice seedling pot in a new first insect-raising cage, and repeating the step 2.1-2.2;
step three, performing a first step of cleaning the substrate,
transplanting the rice seedling pot with the 1-day-old nilaparvata lugens ova obtained in the second step into a beekeeping cage placed in a rice lice larva inoculation and culture room, placing 10 pots in each cage of the beekeeping cage, inoculating 500 rice lice larva females and 300 rice lice larva males in each cage of the beekeeping cage, and marking the inoculation date of the rice lice larva; adding a nutrient solution which can be eaten by the Anagrus nilaparvatae in the beekeeping cage every 24 hours; the environmental conditions of the inoculation and culture room for the Anagrus nilaparvatae are as follows: the temperature is 30 ℃, the relative humidity is 80%, the photoperiod is 14h of illumination, the illumination is 10h of darkness, the illumination intensity is 32000Lux, the fertility of the rice louse young bees is relatively high in the environment with the illumination intensity of 32000Lux, more than 500 brown planthopper eggs can be parasitized by every 50 rice louse young bees, the average egg production amount is about 10 eggs/female, the average egg production amount of the rice louse young bees is about 6 eggs/female in the environment with the illumination intensity of 12800Lux and the same other conditions, the fertility of the rice louse young bees can be kept at a normal level at 30 ℃, the egg production amount is about 18 eggs/female, the service life is shortened to 1.2d, the egg production time is only 1/3 of the service life of the rice louse young bee bees at 25 ℃, the egg production time is centralized, the offspring development process is more uniform, and the large-scale production is facilitated;
under the same other conditions, the egg laying amount of the small rice-louse larvae on the eggs of the brown planthoppers at 1-day age is about 20 eggs/female, and the egg laying amount on the eggs of the brown planthoppers at 3-day age and 5-day age is only 16 eggs/female and 9 eggs/female, so that although the small rice-louse larvae can lay eggs on the eggs of the brown planthoppers at the larger egg age, the small rice-louse larvae lay eggs on the fresh eggs of the brown planthoppers better under the same conditions, rice seedlings with the eggs of the brown planthoppers at 1-day age are adopted for the small rice-louse larvae to lay eggs, the egg laying amount of the small rice-louse larvae on the eggs of the brown planthoppers can be effectively improved, and the yield of the small rice-louse larvae can be improved;
under the same other conditions of the embodiment, when the bee inoculation male-female ratio is 1.6, the ratio of offspring female is about 0.75; when the female-male ratio of the inoculated bees is 1; when the female-male ratio of the inoculated bees is 1.2, the female ratio of the offspring is only 0.69; when the female-male ratio of the inoculated bees is 1, the female ratio of the offspring is about 0.75; the male nilaparvata lugens can be crossly bred with another female nilaparvata lugens rapidly for the next time after the male nilaparvata lugens and the female nilaparvata lugens complete one-time copulation (the copulation time is short), the male nilaparvata lugens can be copulated with the female nilaparvata lugens for the next time by arranging the brood cage to access the female ratio of the nilaparvata lugens to be 1.
Wherein the area of the inoculation and culture room of the Anagrus nilaparvatae is preferably 18m 2 (6 m long x 3m wide) and can be used for placing three rows of cage placing frames, wherein the first row is provided with 10 cage placing frames, the second row is provided with 9 cage placing frames, the third row is provided with 8 cage placing frames, and each cage placing frame is provided with three layers, so that 81 bee keeping cages can be placed in the rice lice tassel wasp inoculation and culture room;
step four, a, collecting bee pupae: collecting brown planthopper nymphs on rice seedlings (the brown planthopper ova which are not parasitized in the rice seedlings can be hatched successively, usually, the brown planthopper ova are all developed into nymphs within 7d, and part of the brown planthopper ova can be developed into nymphs in advance (7 d is not needed)), inoculating the brown planthopper nymphs collected from the bee-keeping cages onto the rice seedling pots obtained in the first step, placing the rice seedling pots in second insect-keeping cages in the brown planthopper inoculation and culture rooms, and when the brown planthopper nymphs collected from the bee-keeping cages in the second insect-keeping cages are developed to 4 days, replacing the rice seedling pots in the second insect-keeping cages with the rice seedling pots obtained in the first step to avoid that the planthoppers are grown into long-wing planthoppers with low fertility and can be used for continuously producing the brown planthopper nymphs after the brown planthopper in the second insect-keeping cages is grown into adults or supplementing the brown planthopper nymphs with the brown planthopper loss during the production process; in the 4d after the rice seedlings with the brown planthopper eggs are potted with the bees, the rice seedlings with the rice planthopper tassels in the pre-pupation period are placed in a refrigerator at the temperature of 4 ℃ for refrigeration storage, so that the storage time of the rice seedlings with the rice planthopper tassels in the pre-pupation period can reach 60 days to the maximum, and meanwhile, the brown planthopper eggs which are not parasitized in the rice seedlings and do not grow into nymphs die gradually at the temperature of 4 ℃, so that the brown planthopper eggs cannot grow into the nymphs and the adults; each pot of rice seedlings with the rice planthopper tassel trichogramma in the pre-pupation stage can produce 450 head rice planthopper tassel trichogramma, and 340 head rice planthopper tassel trichogramma female bees exist in the rice seedlings; when the method needs to be used, the refrigerated rice seedlings with the rice planthopper tassel bees in the pre-pupation stage are directly placed in the field, so that the rice planthopper tassel bees automatically feather into adult bees to control the pests rice planthopper; when only collecting the bee pupae but not collecting adult bees, dividing the collected rice seedlings with the rice planthopper tassel bees in the pre-pupation period into two parts, wherein one part is used for preventing and controlling the rice planthopper in the field, and the other part is reserved for the production of the next batch of rice planthopper tassel bees;
b, adult bee collection: 8-9 d after the rice seedlings with the brown planthopper eggs are potted with the bees, most rice planthopper tassel bees develop to the cryptogamic stage, the rest rice planthopper tassel bees develop to the later stage of pupae, the rice planthopper bees are in the bee-emergence stage, and before the bees emerge, brown planthopper nymphs in the bee-keeping cages are recovered and collected (the brown planthopper eggs which are not parasitized in the rice seedlings can be continuously and completely hatched, about 250 brown planthopper female nymphs and about 250 brown planthopper male nymphs can be respectively collected in each pot of rice seedlings), the brown planthopper nymphs collected from the bee-keeping cages are inoculated to the rice seedlings obtained in the first step, preferably, the brown planthopper nymphs collected from the two pots of rice seedlings are inoculated to one pot of rice seedlings obtained in the first step, and the rice seedling pots which are inoculated with the brown planthopper nymphs are placed in a second planthopper inoculation and cultivation cage in a cultivation chamber; when the nymphs of the brown planthoppers in the second insect-raising cage grow to 4 years old, replacing the rice seedlings in the second insect-raising cage with the rice seedling pots obtained in the first step, so that the brown planthoppers are prevented from growing into long-wing shapes with low fertility due to malnutrition, and the brown planthoppers in the second insect-raising cage can be used for continuously breeding the brown planthoppers after emerging into adults or supplementing the loss of the brown planthoppers in the process of producing the rice seedlings with the brown planthopper eggs; covering the bee-keeping cage by black shading cloth during the bee-out period, arranging 4 bee-out holes on the top of the black shading cloth, wherein the bee-out holes are round holes, placing transparent tubes for collecting bees in the bee-out holes, adjusting the photoperiod of the inoculation and culture chamber of the small rice lice tassel to 24 hours of illumination and 0 hour of darkness during the bee-out period, and replacing the new transparent tubes every 4 hours; dividing the collected adult bees into two parts, wherein one part is used for releasing field control of rice planthoppers, the other part is used for reserving the adult bees for production of the rice planthopper young bees of the next batch, each pot of rice seedlings with rice planthopper young bee eggs can collect 450 rice planthopper young bees, and 340 rice planthopper young bee bees are arranged in the pot; after the bees are collected, part of the small rice-lice tassel bees are required to be reserved for next production, the ratio of the reserved and released small rice-lice tassel bees is 4; meanwhile, the proportion of two collecting modes of bee pupa collection and adult bee collection is adjusted according to the actual demand of pest control;
thus, each batch of the inoculation and culture chamber for each novalula nilaparvatae can produce 450 × 10 × 81=364500novalula nilaparvatae; if two shelves are added in each beekeeping cage (the number of rice seedling pots in the first breeding step, the number of insect-keeping cages or the shelves added in the cages are adjusted adaptively), 16 rice seedling pots with brown planthopper eggs can be placed in each beekeeping cage, and 450 × 16 × 81=583200 rice tassel small bees can be produced in each batch.
In conclusion, the method can realize the large-batch and continuous production of the rice planthopper larvae through the cultivation of the rice seedlings, the production of the rice seedlings with the nilaparvata lugens ova, the breeding of the rice planthopper larvae and the preservation of the pupae, and can simultaneously recover the bred brown planthoppers and the recovered rice planthopper larvae, realize the sustainable production of the rice planthopper larvae, and can artificially supplement the number of the field rice planthopper larvae apis albae populations in time to prevent and control the rice planthoppers; and the length of the storage time can be determined according to the pest prevention and control requirements by refrigerating and storing the rice seedlings with the rice planthopper larvae in the pupation stage, and when the rice planthoppers are harmful rice, the rice seedlings with the rice planthopper larvae in the pupation stage can be immediately placed in the field, so that the rice planthopper larvae automatically feather into adult bees to prevent and control the rice planthoppers.
In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (9)
1. A large-scale breeding method of rice-louse thysanodes chinensis is characterized by comprising the following steps:
step one, sowing rice seeds subjected to germination accelerating treatment until the rice seeds are exposed to the white in a seedling pot, placing the seedling pot after sowing in a clean rice seedling culture room, and culturing until the seedling stage is reached for later use; wherein the environmental conditions of the clean rice seedling culture room are as follows: the temperature is 25 to 31 ℃, the relative humidity is 70 to 90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is more than 20000Lux;
step two, transplanting the rice seedling pot obtained in the step one into a first insect cage placed in a brown planthopper inoculation and culture room, inoculating brown planthopper adults with a male-female ratio of 1 to the first insect cage, and marking the inoculation date of the brown planthopper adults; after the adult brown planthoppers lay eggs, removing and recovering the adult brown planthoppers from the rice seedlings with the eggs of the brown planthoppers, and using the recovered adult brown planthoppers for inoculating to the rice seedling pots obtained in the step one and feeding in a first insect feeding cage; wherein, the environmental conditions of the brown planthopper inoculation and culture room are as follows: the temperature is 22 to 28 ℃, the relative humidity is 70 to 90%, the light cycle is 16h of illumination and 8h of darkness, and the illumination intensity is 30000 to 33000Lux;
step three, transferring the rice seedling pot with the brown planthopper eggs obtained in the step two into a beekeeping cage placed in a rice planthopper larva inoculation and culture room, inoculating rice planthopper young bees with a female-male ratio of 1; wherein, the environmental conditions of the inoculation and culture room for the Anagrus nilaparvatae are as follows: the temperature is 29 to 31 ℃, the relative humidity is 70 to 90%, the light cycle is 14h of illumination and 10h of darkness, and the illumination intensity is 30000 to 33000Lux;
step four, a, collecting bee pupae: collecting brown planthopper nymphs on the rice seedlings at 2 to 3d after the rice seedlings with the brown planthopper eggs are potted with bees, inoculating the brown planthopper nymphs collected from the bee-keeping cage onto the rice seedling pot obtained in the step one, and placing the rice seedling pot into a second insect-keeping cage in a brown planthopper inoculation and culture room for feeding; in the 4d after the rice seedlings with the brown fly lice eggs are potted with the bees, the rice seedlings with the rice lice tassel bees in the pre-pupation period are placed in a refrigerator at the temperature of 4 ℃ for refrigeration and preservation; when the feed needs to be used, the refrigerated rice seedlings with the rice planthopper tassel wasps in the pre-pupation stage are placed in the field, so that the rice planthopper tassel wasps in the pre-pupation stage automatically feather into adult wasps;
b, adult bee collection: c, after the rice seedlings with the brown planthopper eggs are potted and inoculated with the bees, at 8 th to 9 th days, most of the small rice lice tassel bees grow to the cryptogamic period, the rest of the small rice lice tassel bees grow to the later period of pupae, the small rice lice tassel bees are at the bee emergence period, the brown planthopper nymphs in the bee rearing cages are recovered and collected before the bees emerge, the brown planthopper nymphs collected from the bee rearing cages are inoculated onto the rice seedling pot obtained in the step one, and the rice seedling pot inoculated with the brown planthopper nymphs is placed in a second insect rearing cage in the brown planthopper inoculation and culture chamber for rearing; during the bee-out period, covering the bee-keeping cage by black shading cloth, arranging bee-out holes on the black shading cloth, placing transparent tubes for collecting the bees in the bee-out holes, and simultaneously adjusting the photoperiod of the small rice lice tassel inoculating and culturing room to 24h illumination and 0h darkness during the bee-out period; and dividing the collected adult bees into two parts, wherein one part is used for releasing field control rice planthoppers, and the other part is reserved for production of the rice planthopper thysanoptera in the next batch.
2. The method for large-scale breeding of the Anagrus nilaparvatae according to claim 1, wherein in the second step, the temperature of the brown planthopper inoculation and culture chamber is 25 ℃, and the illumination intensity in the brown planthopper inoculation and culture chamber is 32000Lux.
3. The method for large-scale breeding of the rice-louse thysanodes chinensis as claimed in claim 1, wherein in the second step, 10-day-old rice seedlings are transplanted into the first insect-raising cage to be inoculated with the brown planthopper adults.
4. The large-scale breeding method of the rice planthopper thysanoptera as claimed in claim 1, wherein in the second step, after 3d of inoculation, the rice seedling pot with the eggs of the brown planthopper is inverted to drop the adult brown planthopper, whether the rice seedling with the eggs of the brown planthopper has the residue of the adult brown planthopper is checked, if the adult brown planthopper has the residue, the adult brown planthopper is removed through a pest absorber or a glass finger tube, and all the adult brown planthopper is collected and recovered; and (3) inoculating the brown planthopper imagoes collected from the rice seedling pot with the brown planthopper eggs on the rice seedling pot obtained in the step one, and placing the rice seedling pot with the brown planthopper imagoes in a first insect feeding cage in a brown planthopper inoculation and culture room to feed and lay eggs.
5. The method for large-scale breeding of the small rice-lice tassel bees as claimed in claim 1, wherein in the third step, the temperature of the inoculation and culture room for the small rice-lice tassel bees is 30 ℃, and the illumination intensity of the inoculation and culture room for the small rice-lice tassel bees is 32000Lux.
6. The method for large-scale breeding of the rice-louse wasps according to claim 1, wherein the rice-louse wasps are inoculated to a pot of rice seedlings with 1-day-old nilaparvata lugens eggs in step three.
7. The method for large-scale breeding of the rice planthopper tsingtaurus chinensis as claimed in claim 1, wherein in the fourth step, when the nymphs of the brown planthoppers in the second insect-raising cage are grown to 4 years old, the rice seedling pots in the second insect-raising cage are replaced with the rice seedling pots obtained in the first step.
8. The large-scale breeding method of the rice lice tassel apis as claimed in claim 1, wherein in the first step, the rice seeds are washed clean with clear water, then the rice seeds are placed in a constant temperature incubator at 35 ℃ and soaked in clear water for 24h, then the rice seeds are washed clean again and drained, the wet towels are used for covering the rice seeds, and the rice seeds are cultured in the constant temperature incubator at 35 ℃ for 24h, so that the rice seeds are in a white-exposed state.
9. The method for large-scale breeding of the Anagrus nilaparvatae according to claim 8, wherein in the first step, after sowing, the nutrient solution is added into a nursery pot every 12 hours, and the nutrient solution added each time just submerges rice seeds; normal watering at 4d after sowing.
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| CN103385220A (en) * | 2013-07-25 | 2013-11-13 | 任伟 | Scale breeding method for encarsia formosa |
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| CN105794724A (en) * | 2016-03-14 | 2016-07-27 | 云南省农业科学院农业环境资源研究所 | Method for conveniently observing and feeding rice planthoppers |
| CN111480563A (en) * | 2020-05-29 | 2020-08-04 | 扬州大学 | Water culture method for experimental rice cultivated in greenhouse |
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| CN103385220A (en) * | 2013-07-25 | 2013-11-13 | 任伟 | Scale breeding method for encarsia formosa |
| CN103931564A (en) * | 2014-04-15 | 2014-07-23 | 华中农业大学 | Evaluation method of safety of genetically modified insect resistant rice relative to parasite anagrus nilaparvatae |
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