CN113180011A - Large-scale breeding method for small rice-louse tassels - Google Patents
Large-scale breeding method for small rice-louse tassels Download PDFInfo
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- 238000009395 breeding Methods 0.000 title claims abstract description 25
- 241000209094 Oryza Species 0.000 claims abstract description 269
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 269
- 235000009566 rice Nutrition 0.000 claims abstract description 269
- 241001556089 Nilaparvata lugens Species 0.000 claims abstract description 141
- 241001498622 Cixius wagneri Species 0.000 claims abstract description 65
- 241000257303 Hymenoptera Species 0.000 claims abstract description 65
- 235000013601 eggs Nutrition 0.000 claims abstract description 62
- 241001674048 Phthiraptera Species 0.000 claims abstract description 47
- 238000011081 inoculation Methods 0.000 claims abstract description 43
- 241000256856 Vespidae Species 0.000 claims abstract description 27
- 238000009341 apiculture Methods 0.000 claims abstract description 26
- 241000238631 Hexapoda Species 0.000 claims abstract description 22
- 241001466042 Fulgoromorpha Species 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 16
- 230000019617 pupation Effects 0.000 claims abstract description 16
- 238000005286 illumination Methods 0.000 claims description 24
- 230000001488 breeding effect Effects 0.000 claims description 15
- 238000004519 manufacturing process Methods 0.000 claims description 15
- 238000009331 sowing Methods 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 11
- 241001414989 Thysanoptera Species 0.000 claims description 10
- 241000607479 Yersinia pestis Species 0.000 claims description 10
- 230000007613 environmental effect Effects 0.000 claims description 9
- 230000000384 rearing effect Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 241000584992 Anagrus Species 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 230000035784 germination Effects 0.000 claims description 4
- 239000006096 absorbing agent Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000005057 refrigeration Methods 0.000 claims description 3
- 241000209490 Nymphaea Species 0.000 claims 1
- 238000003860 storage Methods 0.000 abstract description 5
- 238000010924 continuous production Methods 0.000 abstract description 4
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 239000003048 aphrodisiac agent Substances 0.000 description 15
- 230000002509 aphrodisiac effect Effects 0.000 description 15
- 241000256846 Apis cerana Species 0.000 description 4
- 230000035558 fertility Effects 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 230000027326 copulation Effects 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 241000256836 Apis Species 0.000 description 2
- 208000002720 Malnutrition Diseases 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000001071 malnutrition Effects 0.000 description 2
- 235000000824 malnutrition Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 208000015380 nutritional deficiency disease Diseases 0.000 description 2
- 230000008186 parthenogenesis Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 230000014639 sexual reproduction Effects 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241001470016 Laodelphax Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000176086 Sogatella furcifera Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 238000005580 one pot reaction Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention relates to the technical field of insect feeding, and discloses a large-scale breeding method of rice louse ceratosporus sp, which comprises the following steps of cultivating rice seedlings at a seedling stage; step two, transferring the rice seedling pot obtained in the step one into a first insect cage placed in a brown planthopper inoculation and culture room, inoculating brown planthopper adults with a male-female ratio of 1:1 into the first insect cage, and producing the rice seedling pot with brown planthopper eggs; step three, transferring the rice seedling pot with the brown planthopper eggs obtained in the step two into a beekeeping cage, and inoculating the rice planthopper tassels with a male-female ratio of 1: 0.5-1 into the beekeeping cage, and step four, collecting the bee pupae: placing the rice seedlings with the rice lice tassel wasps in the pre-pupation stage in a refrigerator for cold storage at 4 ℃; collecting adult bees; the method can realize the large-scale and continuous production of the rice planthopper tassel wasps, and can quickly obtain adult rice planthopper swaps to prevent and control the rice planthoppers by refrigerating and storing the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period.
Description
Technical Field
The invention relates to the technical field of insect feeding, in particular to a large-scale breeding method of small rice-louse thysanodes.
Background
The rice planthopper wasps (Anagrus nilaparvatae Pang et Wang) are widely distributed in main production areas of rice in China, and are important egg-parasitic natural enemies of migratory pests, namely rice planthoppers (the main three types of the rice planthoppers are the brown planthoppers (Nilaparata lugens Stal), Sogatella furcifera Horvath and Laodelphax striatusFall, wherein the brown planthoppers are generated and are the heaviest pests, and the Laodelphax striatellus Fall is the second most important pest). However, outbreaks of rice planthopper populations are sudden and destructive, and the peak period of the field rice planthopper thysanoptera is obviously delayed from the large emergence period of the rice planthopper, so that the rice planthopper thysanoptera (parasitic wasp) cannot effectively control the rice planthopper during the outbreaks of the rice planthopper.
Disclosure of Invention
The invention aims to provide a large-scale breeding method for rice planthopper tassel apis cerana, which can realize large-scale and continuous production of the rice planthopper tassel apis cerana and can quickly obtain adult rice planthopper apis cerana apis cerana to prevent and control the rice planthoppers by refrigerating and storing rice seedlings with the rice planthopper tassel apis cerana in a pre-pupation period.
In order to achieve the purpose, the invention provides a large-scale breeding method of the small rice-louse thysanoptera, which comprises the following steps:
step one, sowing rice seeds subjected to germination accelerating treatment until the rice seeds are exposed to the white in a seedling pot, placing the seedling pot after sowing in a clean rice seedling culture room, and culturing until the seedling stage is reached for later use; wherein the environmental conditions of the clean rice seedling culture room are as follows: the temperature is 25-31 ℃, the relative humidity is 70-90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is more than 20000 Lux;
step two, transferring the rice seedling pot obtained in the step one into a first insect cage placed in a brown planthopper inoculation and culture room, inoculating brown planthopper adults with a male-female ratio of 1:1 into the first insect cage, and marking the inoculation date of the brown planthopper adults; after the brown planthoppers lay eggs, removing and recovering brown planthopper adults from the rice seedlings with the brown planthopper eggs, and using the recovered brown planthopper adults for inoculating to the rice seedling pots obtained in the step one and feeding in a first insect feeding cage; wherein, the environmental conditions of the brown planthopper inoculation and culture room are as follows: the temperature is 22-28 ℃, the relative humidity is 70-90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is 30000-33000 Lux;
step three, transferring the rice seedling pot with the brown planthopper eggs obtained in the step two into a beekeeping cage placed in a rice planthopper larva inoculation and culture room, inoculating rice planthopper larvae with a female-male ratio of 1: 0.5-1 into the beekeeping cage, marking the inoculation date of the rice planthopper larvae, and periodically adding a nutrient solution which can be eaten by the rice planthopper larvae into the beekeeping cage; the environmental conditions of the inoculation and culture room for the small rice lice tassel bees are as follows: the temperature is 29-31 ℃, the relative humidity is 70-90%, the photoperiod is 14h of illumination and 10h of darkness, and the illumination intensity is 30000-33000 Lux;
step four, a, collecting bee pupae: collecting brown planthopper nymphs on the rice seedlings 2-3 days after the rice seedlings with the brown planthopper eggs are potted with the bees, inoculating the brown planthopper nymphs collected from the bee-keeping cages onto the rice seedling pots obtained in the first step, and placing the rice seedling pots in a second insect-keeping cage in a brown planthopper inoculation and culture room for feeding; in the 4d after the rice seedlings with the brown fly lice eggs are potted with the bees, the rice seedlings with the rice lice tassel bees in the pre-pupation period are placed in a refrigerator at the temperature of 4 ℃ for refrigeration and preservation; when the cold-stored rice seedlings with the rice planthopper tassel wasps in the pre-pupation stage are needed to be used, the rice planthopper tassel wasps are placed in the field, and the rice planthopper tassel wasps are enabled to be automatically feathered into adult wasps;
b, adult bee collection: 8-9 d after the rice seedlings with the brown planthopper eggs are potted with the bees, most rice lice tassel bees grow to the cryptogamic period, the rest rice lice tassel bees grow to the later period of pupae, the rice lice tassel bees are in the bee emergence period, the brown planthopper nymphs in the bee rearing cages are recovered and collected before the bees emerge, the brown planthopper nymphs collected from the bee rearing cages are inoculated to the rice seedling pots obtained in the first step, and the rice seedling pots which are inoculated with the brown planthopper nymphs are placed in a second insect rearing cage in a brown planthopper inoculation and culture room for rearing; during the bee-out period, covering the bee-keeping cage by black shading cloth, arranging bee-out holes on the black shading cloth, placing transparent tubes for collecting the bees in the bee-out holes, and simultaneously adjusting the photoperiod of the small rice lice tassel inoculating and culturing room to 24h illumination and 0h darkness during the bee-out period; and dividing the collected adult bees into two parts, wherein one part is used for releasing field control rice planthoppers, and the other part is reserved for production of the rice planthopper thysanoptera in the next batch.
As a preferable scheme of the invention, in the second step, the temperature of the brown planthopper inoculation and culture chamber is 25 ℃, and the illumination intensity in the brown planthopper inoculation and culture chamber is 32000 Lux.
As a preferable scheme of the invention, in the second step, rice seedlings of 10 days old are transplanted into the first insect rearing cage to be inoculated with the brown planthopper adults.
As a preferable scheme of the invention, in the second step, after the 3 rd step of inoculation, inverting the rice seedlings with the eggs of the brown planthoppers to enable the adult brown planthoppers to fall off, checking whether the rice seedlings with the eggs of the brown planthoppers have the residual adult brown planthoppers or not, if the residual brown planthoppers exist, removing the adult brown planthoppers through a pest absorber or a glass finger-shaped tube, and collecting and recycling all the adult brown planthoppers; and (3) inoculating the brown planthopper imagoes collected from the rice seedling pot with the brown planthopper eggs on the rice seedling pot obtained in the step one, and placing the rice seedling pot with the brown planthopper imagoes in a first insect feeding cage in a brown planthopper inoculation and culture room to feed and lay eggs.
As a preferable scheme of the invention, in the third step, the ratio of the male to the female of the small rice-lice tassel bees inoculated to the beekeeping cage is controlled to be 1: 0.6.
In a preferred embodiment of the invention, in step three, the temperature of the inoculation and culture chamber for the Anagrus nilaparvatae is 30 ℃, and the illumination intensity of the inoculation and culture chamber for the Anagrus nilaparvatae is 32000 Lux.
As a preferable scheme of the invention, in the third step, the rice seedling pot with 1-day-old nilaparvata lugens eggs is adopted to be inoculated to the rice planthoppers.
As a preferable scheme of the present invention, in the fourth step, when the nymphs of brown planthoppers in the second insect cages are grown to 4 years old, the rice seedling pots in the second insect cages are replaced with the rice seedling pots obtained in the first step.
As a preferable scheme of the invention, in the step one, firstly, rice seeds are washed clean by clear water, blighted grains and impurities are removed, then the rice seeds are placed in a constant temperature incubator at 35 ℃ and soaked for 24 hours by clear water, the rice seeds are washed again and drained, the rice seeds are covered by a wet towel and are cultured for 24 hours in the constant temperature incubator at 35 ℃, and the rice seeds are in an exposed white state.
As a preferable scheme of the invention, in the step one, after sowing, nutrient solution is added into a seedling raising pot every 12 hours, and the nutrient solution added each time just submerges the rice seeds; normal watering at 4d after sowing.
Compared with the prior art, the large-scale breeding method for the rice lice thysanoptera in the embodiment of the invention has the beneficial effects that:
according to the method, by cultivating the rice seedlings, producing the rice seedlings with the nilaparvata lugens ova, breeding the nilaparvata lugens and storing the pupae, the large-batch and continuous production of the nilaparvata lugens can be realized, the bred nilaparvata lugens and the recovered nilaparvata lugens are recovered at the same time, the sustainable production of the nilaparvata lugens is realized, and the number of the field nilaparvata lugens apis in time can be supplemented manually to prevent and control the nilaparvata lugens; and the length of the storage time can be determined according to the pest prevention and control requirements by refrigerating and storing the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period, and when the rice planthoppers are harmful rice, the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period can be immediately placed in the field, so that the rice planthopper tassel wasps automatically feather into adult wasps to quickly prevent and control the rice planthoppers.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The embodiment of the invention provides a large-scale breeding method of Anagrus nilaparvatae, which comprises the following steps:
in the first step of the method,
1.1, washing rice seeds with clear water, removing blighted grains and impurities, then placing the rice seeds in a constant-temperature incubator at 35 ℃ to be soaked for 24 hours with clear water, then washing again, draining water, covering the rice seeds with a wet towel, and culturing for 24 hours in the constant-temperature incubator at 35 ℃ to ensure that the rice seeds are subjected to germination acceleration until the rice seeds are in a white exposed state; wherein the rice seeds are temperature-sensitive conventional rice variety Huanghuazhan seeds;
1.2, sowing the rice seeds subjected to germination accelerating treatment until the rice seeds are exposed to white in seedling pots, and sowing about 1300 rice seeds (the dry mass of the rice seeds is 25g) in the exposed-white state in each seedling pot, wherein the rice seeds are uniformly potted and have moderate tightness; the seedling raising pot adopts the following specifications: a white plastic pot 20cm long by 14cm wide by 5cm high; the seedling cultivation pot after sowing is placed in a clean rice seedling cultivation room, and the environmental conditions of the clean rice seedling cultivation room are as follows: the temperature is 28 ℃, the relative humidity is 80%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is more than 20000 Lux;
1.3, after sowing, adding nutrient solution into the seedling raising pot every 12 hours, wherein the nutrient solution added each time just submerges the rice seeds, and the root systems of the rice seedlings are loosely combined, so that the nutrient solution of the rice seedlings is prevented from being dispersed when the nutrient solution is added; normal watering at 4d after sowing; the growth height of the rice seedlings on the 10 th day after sowing is 8-9 cm for later use; wherein 972 pots of rice seedlings are produced in each batch, wherein 810 pots of rice planthoppers are used for producing the small tassels of the rice planthoppers, and 162 pots of rice planthoppers are used for producing the brown planthoppers;
in the second step, the first step is that,
2.1, moving the 10-day-old rice seedling pots obtained in the step one into first insect cages placed in a brown planthopper inoculation and culture room, placing 10 pots in each first insect cage, inoculating brown planthopper adults with a male-female ratio of 1:1 into each first insect cage, inoculating 500 brown planthopper females and 500 brown planthopper males into each first insect cage, and marking the inoculation date of the brown planthopper adults; the environmental conditions of the brown planthopper inoculation and culture room are as follows: the temperature is 25 ℃, the relative humidity is 80%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is 32000 Lux; since the brown planthopper likes warm and wet, if the temperature exceeds a proper range, the reproduction of the brown planthopper is influenced, and since the egg laying amount of the imagoes is maximum at 24.6 ℃, 25 ℃ is selected as the culture temperature; wherein the area of the brown planthopper inoculation and culture chamber is 18m2At least 81 insect cages can be placed;
2.2, after the 3 rd day of inoculation, inverting the rice seedling pot with the brown planthopper eggs to enable the brown planthopper adults to fall off, checking whether the rice seedlings with the brown planthopper eggs have brown planthopper adult residues or not, if the brown planthopper adult residues exist, removing the brown planthopper adults through a pest absorber or a glass finger tube, and collecting and recycling all the brown planthopper adults so as to obtain the rice seedling pot with the 1-day-old brown planthopper eggs;
2.3, inoculating the brown planthopper adults recovered from the step 2.2 onto the rice seedling pot obtained in the step one, feeding the rice seedling pot in a new first insect-raising cage, and repeating the steps 2.1-2.2;
step three, performing a first step of cleaning the substrate,
transplanting the rice seedling pot with the 1-day-old nilaparvata lugens ova obtained in the second step into a bee-keeping cage placed in a rice lice larva inoculation and culture room, placing 10 pots in each cage of bee-keeping cage, inoculating the rice lice larva with a male-female ratio of 1:0.6 into the bee-keeping cage, inoculating 500 heads of the rice lice larva female bees and 300 heads of the rice lice larva male bees into each cage of bee-keeping cage, and marking the inoculation date of the rice lice larva; adding nutrient solution which can be eaten by the small rice-louse thysanodes in the beekeeping cage every 24 hours; the environmental conditions of the inoculation and culture room for the small rice lice tassel are as follows: the temperature is 30 ℃, the relative humidity is 80%, the photoperiod is 14h of illumination, the lighting intensity is 32000Lux, the fertility of the rice louse young bees is relatively high in the environment with the lighting intensity of 32000Lux, more than 500 eggs of nilaparvata lugens can be parasitized by every 50 female bees of the rice louse young bees, the average egg production amount is about 10 eggs/female, the average egg production amount of the rice louse young bees is about 6 eggs/female in the environment with the lighting intensity of 12800Lux and the same other conditions, the fertility of the rice louse young bees can be kept at a normal level at 30 ℃, the egg production amount is about 18 eggs/female, the service life is shortened to 1.2d, the average egg production amount is 1/3 of the service life of the rice louse young bee adult bees at 25 ℃, the egg production time is centralized, the offspring development process is more uniform, and the large-scale production is facilitated;
under the same other conditions, the egg laying amount of the small rice-louse larvae on the eggs of the brown planthoppers at 1-day age is about 20 eggs/female, and the egg laying amount on the eggs of the brown planthoppers at 3-day age and 5-day age is only 16 eggs/female and 9 eggs/female, so that although the small rice-louse larvae can lay eggs on the eggs of the brown planthoppers at the larger egg age, the small rice-louse larvae lay eggs on the fresh eggs of the brown planthoppers better under the same conditions, rice seedlings with the eggs of the brown planthoppers at 1-day age are adopted for the small rice-louse larvae to lay eggs, the egg laying amount of the small rice-louse larvae on the eggs of the brown planthoppers can be effectively improved, and the yield of the small rice-louse larvae can be improved;
under other conditions of the embodiment, when the ratio of the male to the female of the inoculated bee is 1:0.6, the ratio of the female of the offspring is about 0.75; when the male-female ratio of the inoculated bees is 1:0.5, the female ratio of the offspring is about 0.72; when the male-female ratio of the inoculated bees is 1:0.2, the female ratio of the offspring is only 0.69; when the male-female ratio of the inoculated bees is 1:1, the female ratio of the offspring is about 0.75; because the rice lice aphrodisiac can perform the sexual reproduction and the parthenogenesis, when the number of the male rice lice aphrodisiac is too small, the female rice lice aphrodisiac can perform the parthenogenesis, and after the male rice lice aphrodisiac and the female rice lice aphrodisiac complete one-time copulation (the copulation time is short), the male rice lice aphrodisiac can rapidly perform the next copulation with the female rice lice aphrodisiac, the male rice lice aphrodisiac male bee is connected with a bee cage to set the male ratio of the rice lice aphrodisiac to be 1:0.6, the number of the rice lice aphrodisiac male and the female rice lice aphrodisiac female bee can be ensured to be sufficient, the female rice lice aphrodisiac female bees can be prevented from reproducing to reduce the ratio of the offspring and enable the offspring to degenerate, the female rice lice aphrodisiac female bees of the rice lice aphrodisiac can perform the sexual reproduction basically, the number of the produced offspring of the rice lice aphrodisiac female bees can be maximized, the effect of the rice lice female rice lice flies can be effectively improved, and the number of the rice lice female bees can be ensured on the premise that the female rice lice female bees can be maximized, the male-female ratio of the inoculated rice lice tassel wasps is set to be 1:0.6, so that the investment of the male wasps can be reduced, and the cost is further reduced.
Wherein the area of the inoculation and culture room of the Anagrus nilaparvatae is preferably 18m2(6 m long x 3m wide) and can be used for placing three rows of cage placing frames, wherein the first row is provided with 10 cage placing frames, the second row is provided with 9 cage placing frames, the third row is provided with 8 cage placing frames, and each cage placing frame is provided with three layers, so that 81 bee keeping cages can be placed in the rice lice tassel wasp inoculation and culture room;
step four, a, collecting bee pupae: and (2) to (3) d after the rice seedlings with the brown planthopper eggs are potted with the bees, recovering and collecting the brown planthopper nymphs on the rice seedlings (the brown planthopper eggs which are not parasitized in the rice seedlings can be hatched successively, usually, the brown planthopper eggs are all developed into nymphs within 7d, and part of the brown planthopper eggs are developed into nymphs in advance (no 7d)), inoculating the brown planthopper nymphs collected from the bee-keeping cages to the rice seedling pots obtained in the first step, placing the rice seedling pots in a second insect-keeping cage in a brown planthopper inoculation and culture room for breeding, when the brown planthopper nymphs collected from the bee-keeping cages in the second insect-keeping cage are grown to 4 years old, the rice seedling pot in the second insect-keeping cage is replaced by the rice seedling pot obtained in the first step, so that the brown planthopper is prevented from growing into a long-wing type with lower fertility due to malnutrition, and the brown planthopper in the second insect-keeping cage can be used for continuously breeding the brown planthopper after emerging into an adult or supplementing the loss of the brown planthopper in the process of producing the rice seedlings with the brown planthopper eggs; in the 4d after the rice seedlings with the brown planthopper eggs are potted with the bees, the rice seedlings with the rice planthopper tassels in the pre-pupation period are placed in a refrigerator at the temperature of 4 ℃ for refrigeration storage, so that the storage time of the rice seedlings with the rice planthopper tassels in the pre-pupation period can reach 60 days to the maximum, and meanwhile, the brown planthopper eggs which are not parasitized in the rice seedlings and do not grow into nymphs die gradually at the temperature of 4 ℃, so that the brown planthopper eggs cannot grow into the nymphs and the adults; each pot of rice seedlings with the rice planthopper tassel wasps in the pre-pupation period can produce 450 rice planthopper tassel wasps, and 340 rice planthopper tassel wasp female bees are among the 450 rice planthopper tassel wasps; when the method needs to be used, the refrigerated rice seedlings with the rice planthopper tassel bees in the pre-pupation stage are directly placed in the field, so that the rice planthopper tassel bees automatically feather into adult bees to control the pests rice planthopper; when only collecting the bee pupae but not collecting adult bees, dividing the collected rice seedlings with the rice planthopper tassel bees in the pre-pupation period into two parts, wherein one part is used for preventing and controlling the rice planthopper in the field, and the other part is reserved for the production of the next batch of rice planthopper tassel bees;
b, adult bee collection: 8-9 d after the rice seedlings with the brown planthopper eggs are potted with the bees, most rice planthopper tassels develop to a cryptogamic stage, the rest rice planthopper tassels develop to a later stage of pupae, the rice planthopper tassels are in a bee-emergence stage, and before bee emergence, brown planthopper nymphs in bee-keeping cages are recovered and collected (the brown planthopper eggs which are not parasitized in the rice seedlings can be continuously and completely hatched, about 250 brown planthopper female nymphs and about 250 brown planthopper male nymphs can be respectively collected in each pot of rice seedlings), the brown planthopper nymphs collected from the bee-keeping cages are inoculated to the rice seedlings obtained in the first step, the brown planthopper nymphs collected from the two pots of rice seedlings are preferably inoculated to one pot of rice seedlings obtained in the first step, and the rice seedling pots which are inoculated with the brown planthopper nymphs are placed in a second planthopper inoculation and cultivation cage in a cultivation chamber; when the nymphs of the brown planthoppers in the second insect-raising cage grow to 4 years old, replacing the rice seedlings in the second insect-raising cage with the rice seedling pots obtained in the first step, so that the brown planthoppers are prevented from growing into long-wing shapes with low fertility due to malnutrition, and the brown planthoppers in the second insect-raising cage can be used for continuously breeding the brown planthoppers after emerging into adults or supplementing the loss of the brown planthoppers in the process of producing the rice seedlings with the brown planthopper eggs; covering the bee-keeping cage by black shading cloth during the bee-out period, arranging 4 bee-out holes on the top of the black shading cloth, wherein the bee-out holes are round holes, placing transparent tubes for collecting bees in the bee-out holes, adjusting the photoperiod of the inoculation and culture chamber of the small rice lice tassel to 24 hours of illumination and 0 hour of darkness during the bee-out period, and replacing the new transparent tubes every 4 hours; dividing the collected adult bees into two parts, wherein one part is used for releasing field control of rice planthoppers, the other part is used for reserving the adult bees for production of the rice planthopper young bees of the next batch, each pot of rice seedlings with rice planthopper young bee eggs can collect 450 rice planthopper young bees, and 340 rice planthopper young bee bees are arranged in the pot; after the bees are collected, a part of small rice-louse bees are required to be reserved for next production, the ratio of the reserved and released small rice-louse bees is 4:23, the reserved small rice-louse bees at each time can be used for next production of the small rice-louse bees, and if the small rice-louse bees are bred for a long time, the small rice-louse bees used for spawning need to be supplemented with the population from the field for rejuvenation; meanwhile, the proportion of two collecting modes of bee pupa collection and adult bee collection is adjusted according to the actual demand of pest control;
so that each batch of the inoculation and culture chamber of each small rice lice tassel can produce 450 × 10 × 81 ═ 364500 small rice lice tassel bees; if two layers of frames are added in each beekeeping cage (the number of rice seedling pots in the first breeding step, the number of insect-keeping cages or the number of the layers of frames added in the first breeding step are adjusted adaptively), 16 rice seedling pots with brown planthopper eggs can be placed in each beekeeping cage, and 450 × 16 × 81-583200 rice planthopper tassels can be produced in each batch.
In conclusion, the method can realize the large-batch and continuous production of the rice planthopper larvae through the cultivation of the rice seedlings, the production of the rice seedlings with the nilaparvata lugens ova, the breeding of the rice planthopper larvae and the preservation of the pupae, and can simultaneously recover the bred brown planthoppers and the recovered rice planthopper larvae, realize the sustainable production of the rice planthopper larvae, and can artificially supplement the number of the field rice planthopper larvae apis albae populations in time to prevent and control the rice planthoppers; and the length of the storage time can be determined according to the pest prevention and control requirements by refrigerating and storing the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period, and when the rice planthoppers are harmful rice, the rice seedlings with the rice planthopper tassel wasps in the pre-pupation period can be immediately placed in the field, so that the rice planthopper tassel wasps automatically feather into adult wasps to prevent and control the rice planthoppers.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and substitutions can be made without departing from the technical principle of the present invention, and these modifications and substitutions should also be regarded as the protection scope of the present invention.
Claims (10)
1. A large-scale breeding method of rice-louse thysanodes chinensis is characterized by comprising the following steps:
step one, sowing rice seeds subjected to germination accelerating treatment until the rice seeds are exposed to the white in a seedling pot, placing the seedling pot after sowing in a clean rice seedling culture room, and culturing until the seedling stage is reached for later use; wherein the environmental conditions of the clean rice seedling culture room are as follows: the temperature is 25-31 ℃, the relative humidity is 70-90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is more than 20000 Lux;
step two, transferring the rice seedling pot obtained in the step one into a first insect cage placed in a brown planthopper inoculation and culture room, inoculating brown planthopper adults with a male-female ratio of 1:1 into the first insect cage, and marking the inoculation date of the brown planthopper adults; after the brown planthoppers lay eggs, removing and recovering brown planthopper adults from the rice seedlings with the brown planthopper eggs, and using the recovered brown planthopper adults for inoculating to the rice seedling pots obtained in the step one and feeding in a first insect feeding cage; wherein, the environmental conditions of the brown planthopper inoculation and culture room are as follows: the temperature is 22-28 ℃, the relative humidity is 70-90%, the photoperiod is 16h of illumination and 8h of darkness, and the illumination intensity is 30000-33000 Lux;
step three, transferring the rice seedling pot with the brown planthopper eggs obtained in the step two into a beekeeping cage placed in a rice planthopper larva inoculation and culture room, inoculating rice planthopper larvae with a female-male ratio of 1: 0.5-1 into the beekeeping cage, marking the inoculation date of the rice planthopper larvae, and periodically adding a nutrient solution which can be eaten by the rice planthopper larvae into the beekeeping cage; wherein, the environmental conditions of the inoculation and culture room for the Anagrus nilaparvatae are as follows: the temperature is 29-31 ℃, the relative humidity is 70-90%, the photoperiod is 14h of illumination and 10h of darkness, and the illumination intensity is 30000-33000 Lux;
step four, a, collecting bee pupae: collecting brown planthopper nymphs on the rice seedlings 2-3 days after the rice seedlings with the brown planthopper eggs are potted with the bees, inoculating the brown planthopper nymphs collected from the bee-keeping cages onto the rice seedling pots obtained in the first step, and placing the rice seedling pots in a second insect-keeping cage in a brown planthopper inoculation and culture room for feeding; in the 4d after the rice seedlings with the brown fly lice eggs are potted with the bees, the rice seedlings with the rice lice tassel bees in the pre-pupation period are placed in a refrigerator at the temperature of 4 ℃ for refrigeration and preservation; when the cold-stored rice seedlings with the rice planthopper tassel wasps in the pre-pupation stage are needed to be used, the rice planthopper tassel wasps are placed in the field, and the rice planthopper tassel wasps are enabled to be automatically feathered into adult wasps;
b, adult bee collection: 8-9 d after the rice seedlings with the brown planthopper eggs are potted with the bees, most rice lice tassel bees grow to the cryptogamic period, the rest rice lice tassel bees grow to the later period of pupae, the rice lice tassel bees are in the bee emergence period, the brown planthopper nymphs in the bee rearing cages are recovered and collected before the bees emerge, the brown planthopper nymphs collected from the bee rearing cages are inoculated to the rice seedling pots obtained in the first step, and the rice seedling pots which are inoculated with the brown planthopper nymphs are placed in a second insect rearing cage in a brown planthopper inoculation and culture room for rearing; during the bee-out period, covering the bee-keeping cage by black shading cloth, arranging bee-out holes on the black shading cloth, placing transparent tubes for collecting the bees in the bee-out holes, and simultaneously adjusting the photoperiod of the small rice lice tassel inoculating and culturing room to 24h illumination and 0h darkness during the bee-out period; and dividing the collected adult bees into two parts, wherein one part is used for releasing field control rice planthoppers, and the other part is reserved for production of the rice planthopper thysanoptera in the next batch.
2. The method for large-scale breeding of the rice planthopper thysanoptera as claimed in claim 1, wherein in the second step, the temperature of the brown planthopper inoculation and culture chamber is 25 ℃, and the illumination intensity in the brown planthopper inoculation and culture chamber is 32000 Lux.
3. The method for large-scale breeding of the rice-louse thysanodes chinensis as claimed in claim 1, wherein in the second step, 10-day-old rice seedlings are transplanted into the first insect-raising cage to be inoculated with the brown planthopper adults.
4. The large-scale breeding method of the rice planthopper thysanoptera as claimed in claim 1, wherein in the second step, after 3d of inoculation, the rice seedling pot with the eggs of the brown planthopper is inverted to drop the adult brown planthopper, whether the rice seedling with the eggs of the brown planthopper has the residue of the adult brown planthopper is checked, if the adult brown planthopper has the residue, the adult brown planthopper is removed through a pest absorber or a glass finger tube, and all the adult brown planthopper is collected and recovered; and (3) inoculating the brown planthopper imagoes collected from the rice seedling pot with the brown planthopper eggs on the rice seedling pot obtained in the step one, and placing the rice seedling pot with the brown planthopper imagoes in a first insect feeding cage in a brown planthopper inoculation and culture room to feed and lay eggs.
5. The method for large-scale breeding of the rice-lice-thysanoptera as claimed in claim 1, wherein in the third step, the ratio of male to female of the rice-lice-thysanoptera is controlled to be 1: 0.6.
6. The method for large-scale breeding of the small rice-lice tassel bees as claimed in claim 1, wherein in the third step, the temperature of the inoculation and culture room for the small rice-lice tassel bees is 30 ℃, and the illumination intensity of the inoculation and culture room for the small rice-lice tassel bees is 32000 Lux.
7. The method for large-scale breeding of the rice-louse wasps according to claim 1, wherein the rice-louse wasps are inoculated to a pot of rice seedlings with 1-day-old nilaparvata lugens eggs in step three.
8. The method for large-scale breeding of the rice-louse thysanodes as claimed in claim 1, wherein in the fourth step, when the nymphaea fuliginosus nymphs in the second insect-raising cage are grown to 4 years old, the rice seedling pots in the second insect-raising cage are replaced with the rice seedling pots obtained in the first step.
9. The method for large-scale breeding of the rice lice thysanoptera as claimed in claim 1, wherein in the first step, the rice seeds are washed clean with clean water, then the rice seeds are placed in a constant temperature incubator at 35 ℃ and soaked in clean water for 24 hours, and then the rice seeds are drained after being washed clean again, covered with a wet towel and cultured in the constant temperature incubator at 35 ℃ for 24 hours, so that the rice seeds are in an exposed white state.
10. The method for the scaled breeding of the rice lice thysanoptera as claimed in claim 9, wherein in the first step, after sowing, the nutrient solution is added into the nursery pot every 12 hours, and the nutrient solution added each time just submerges the rice seeds; normal watering at 4d after sowing.
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| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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