CN1397159A - Technology for industrially culturing trichogramma with gelechiid ova - Google Patents
Technology for industrially culturing trichogramma with gelechiid ova Download PDFInfo
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- CN1397159A CN1397159A CN02129344.9A CN02129344A CN1397159A CN 1397159 A CN1397159 A CN 1397159A CN 02129344 A CN02129344 A CN 02129344A CN 1397159 A CN1397159 A CN 1397159A
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- gelechiid
- ovum
- feed
- honeybee
- adult
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- 238000005516 engineering process Methods 0.000 title claims abstract description 18
- 241000256618 Trichogramma Species 0.000 title claims abstract description 8
- 238000012258 culturing Methods 0.000 title claims description 6
- 239000000428 dust Substances 0.000 claims abstract description 4
- 239000012535 impurity Substances 0.000 claims abstract description 4
- 210000004681 ovum Anatomy 0.000 claims description 70
- 241000256844 Apis mellifera Species 0.000 claims description 46
- 102000002322 Egg Proteins Human genes 0.000 claims description 43
- 108010000912 Egg Proteins Proteins 0.000 claims description 43
- 235000013601 eggs Nutrition 0.000 claims description 25
- 241000209219 Hordeum Species 0.000 claims description 18
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 18
- 238000009395 breeding Methods 0.000 claims description 14
- 230000001488 breeding effect Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 230000005058 diapause Effects 0.000 claims description 6
- 230000003071 parasitic effect Effects 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000000151 deposition Methods 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000012856 packing Methods 0.000 claims description 4
- 238000005057 refrigeration Methods 0.000 claims description 4
- 230000008030 elimination Effects 0.000 claims description 3
- 238000003379 elimination reaction Methods 0.000 claims description 3
- 230000005071 geotropism Effects 0.000 claims description 3
- 230000007774 longterm Effects 0.000 claims description 3
- 210000001161 mammalian embryo Anatomy 0.000 claims description 3
- 239000003595 mist Substances 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 claims description 2
- 241000238631 Hexapoda Species 0.000 claims description 2
- 230000024241 parasitism Effects 0.000 claims description 2
- 239000000575 pesticide Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 235000010725 Vigna aconitifolia Nutrition 0.000 abstract 1
- 244000042325 Vigna aconitifolia Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 3
- 229910000831 Steel Inorganic materials 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000032669 eclosion Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000011087 paperboard Substances 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 241000256837 Apidae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Jellies, Jams, And Syrups (AREA)
Abstract
A technology for reproducing trichogramma with gelechiid ova in industrial mode incldues light-shielding vertical raising, automatical collection of mothes, removing dust and impurities and continuous reproduction.
Description
Technical field
The present invention relates to a kind of technology of cultivating oophagous trichogrammae, particularly use technology for industrially culturing trichogramma with gelechiid ova, belong to the biological control technical field of pest.
Background technology
In the prior art, do vector at the large-scale ovum of the main use of production breeding of China oophagous trichogrammae, its shortcoming is to prevent that the agricultural pests effect is low, and range of application is narrow.The technology of using ovule to do vector is also arranged in recent years, is the patent application of CN1253723A as patent publication No., but the feed of selecting for use is subjected to influence that ambient temperature and humidity changes too greatly; Add water behind the feed disinfection and easily cause secondary pollution; Can not realize once connecing worm and withhold moth more, the collection rate and the cleannes of gelechiid ova are low; Because the structure of numerous beehive causes and can not improve the uniformity and the unit space bee yield that spreads ovum, numerous honeybee quality is low.
Summary of the invention
The objective of the invention is to overcome above deficiency, a kind of technology for industrially culturing trichogramma with gelechiid ova of using be provided, comprise following technical process:
(1), feed is selected: selecting the barley of band Ying shell for use is feed, and utilizing has shell barley self performance of keeping humidity to reduce the influence that feed is subjected to the variation of ambient temperature and humidity.
(2), sterilizing pesticide: the screen frame formula feed frame (two sides is the wooden frame of stainless (steel) wire) of packing into after feed (barley) the elutriation impurity elimination is heated in high-temperature disinfection cabinet, temperature remain on 85～110 ℃ 60～90 minutes, kill insect and worm's ovum in the feed, take out back barley water content and remain on 14～20%.
(3) connect ovum parasitism and larva raising: the ratio of gelechiid ova in 1: 1000 inserted in the feed that disinfects in the larva raising chamber, feed thickness 3～4cm, the receptacle temperature keeps 28 ± 2 ℃, relative moisture keeps 85 ± 5%, unglazed photograph, for preventing feed mold under the super-humid conditions, receptacle was opened the uviol lamp sterilization weekly 2 times each 25～35 minutes.Connect 3 weeks of ovum, the feed that will contain gelechiid is put into the imago breeding frame, changes the adult collecting box in the lump over to and prepares to collect adult.
(4) raising of adult and collection: in the imago breeding chamber, temperature keeps 28 ± 2 ℃, and relative moisture keeps 80 ± 5%, and unglazed photograph, feed be from changing the adult collecting box over to eliminating 3 months, 3 generations of intermediate collection adult; The adult collecting box is a lower hopper, and the vertical slot in top is inserted room imago breeding frame, utilizes gelechiid adult geotropism to collect adult automatically, and receive every day changes gelechiid 1～2 time, changes the gelechiid adult over to the adult cage of laying eggs.
(5) collection of ovum and cleaning: at the gelechiid laying coop, temperature keeps 24 ± 2 ℃, relative moisture keeps 85 ± 5%, unglazed photograph, after the gelechiid adult was poured the cage of laying eggs into, the cage of will laying eggs was placed on the ovum collecting machine, per 2 hours, gathering machine drives the cage rotation 10 minutes of laying eggs automatically, brushes the gelechiid ova on the cage that falls to laying eggs, and the air extractor of opening simultaneously on the gathering machine is removed fine dust such as gelechiid scale; Per 24 hours, the gelechiid ova that will be dropped on the baffle plate by the special messenger collected, with the clear egg apparatus of air-flowing type and sub-sieve cleaning gelechiid ova and store classifiedly.
(6) depositing of ovum: the ovum of depositing numerous honeybee ovum respectively and being used to breed gelechiid with the refrigerator of 4 ± 1 ℃ and 10 ± 1 ℃.
(7) diffusing parasitic bee: in numerous honeybee workshop, temperature keeps 26 ± 2 ℃, relative moisture keeps 75 ± 5%, 14: 10 Light To Dark Ratios, plant honeybee and connect ovum than planting a honeybee by breeding in 1: 15,1: 8～10 propagation production are used honeybee, laying off eggs is sprinkling upon on the aluminium-plastic panel that adds mist, every plate spreads ovum 10～13g, uviol lamp down irradiation used after killing the gelechiid embryo in 30 minutes, and numerous beehive is for being improved to 13 layers of novel numerous beehive of 3 lattice of 3: 1: 3 on the numerous beehive of Morrison basis, single case day numerous honeybee 450～5,000,000, connect the honeybee time between 24～48 hours, take out the mixed pallet that changes over to of parasitic good gelechiid ova after 48 hours and grow in numerous honeybee chamber and make the ovum card after the optimum period or take other measure to handle the back and store.
(8) storage honeybee: short is to deposit under 10 ± 1 ℃ of conditions, changing refrigerator from the placement in 20 ℃ incubator of taking-up back, numerous honeybee chamber over to after half a day before egg parasitoid is deposited deposits, deposit a few days ago to stir every day and store vessel once, prevent the egg parasitoid caking, short-term is deposited with 10 days and is advisable with interior, stores the quality that use after 15～20 days will detect honeybee; Long term storage is to deposit under 3 ± 1 ℃ of conditions, adopts and induces the oophagous trichogrammae diapause, and the way of refrigeration diapause honeybee can be stored 3～6 months, generally uses later in 4～6 months in refrigeration.
Effect of the present invention is: selecting the barley with clever shell for use is feed, reduces the influence that feed is changed by ambient temperature and humidity; With the screen frame barley of sterilizing, reduced and added the probability that water causes secondary pollution behind the feed disinfection; Once connecing worm withholds moth more and has improved the raising operating efficiency; Utilize gelechiid self geotropism to collect adult, reduce the consumption that machinery is received moth; The automatic receipts ovum machine of timing working can effectively improve the collection rate and the cleannes of ovum; Can improve cleaning efficiency with clear egg apparatus of air-flowing type and sub-sieve cleaning gelechiid ova; Use novel numerous beehive and use aluminium-plastic panel to replace paperboard holder ovum, improve the uniformity and the unit space bee yield that spread ovum; Use the granule ovum can breed parasitic field breeding bee of parvule-egg, widen and use the oophagous trichogrammae kind, and the realization anniversary numerous honeybee be beneficial to the personnel of making full use of, equipment, be convenient to accumulate in a large number the honeybee kind.
Embodiment
Further specify below in conjunction with embodiment:
A kind of technology for industrially culturing trichogramma with gelechiid ova of using, comprise following technical process: selecting the barley 135g of band Ying shell for use is feed, the feed frame (two sides is the wooden frame of stainless (steel) wire) of packing into after feed (barley) the elutriation impurity elimination is heated to 100 ℃ in high-temperature disinfection cabinet, keep after 60 minutes taking out that barley is cooled off naturally, drying one day, barley water content 15%.Divide 3 batches (every crowd of 50kg, 1 weeks at interval) feed box of quantitatively packing into the barley that disinfects, change the larva raising chamber in the feed thickness 4cm, every box behind the access gelechiid ova 4.5m1 (about 2.5g) over to and cultivate with every box 2.5kg.Connecing worm will be with the worm feed to change the imago breeding frame over to change the adult collecting box in the lump over to and prepare to collect adult after 3 weeks.Each imago breeding frame can hold the 7.5kg feed, and single adult collecting box can hold 18 frames.Connect worm from first and begin to have the gelechiid adult eclosion after the 28th day, average day collection gelechiid adult 88.9g in later 2 months.Pack into the cage of laying eggs of the adult of collecting is placed on the gelechiid ova gathering machine, temperature remains on 24 ℃, and relative moisture keeps 85%, collects gelechiid ova under the no optical condition, the gelechiid ova that arrive with clear egg apparatus of air-flowing type and sub-sieve clean catch every day, per day receipts ovum 12.2g.Deposit standby by producing the refrigerator that needs respectively 3 ℃ and 10 ℃ the gelechiid ova collected.The 3rd batch of adult eclosion peak adds up to collection gelechiid adult 7115g, gelechiid ova 976g later after 2 months.To raise frame and take out and to be heated to 70 ℃ and to kill residual worm and sorted remaining feed in 1 hour, remaining 4.5kg feed reclaims and uses.
Gelechiid ova evenly is sprinkling upon on the aluminium-plastic panel that adds mist, and irradiation changed in numerous beehive after killing the gelechiid embryo in 30 minutes under uviol lamp, inserted out the kind honeybee of honeybee about 30% in the ratio of honeybee ovum than 1: 10.At 26 ℃, relative moisture keeps 75%, 14: 10 Light To Dark Ratio conditions to take out the mixed pallet that changes over to of parasitic good gelechiid ova after following 24 hours growing after optimum period in numerous honeybee chamber and make the ovum card or take other measure to handle the back storage.
Be sprinkling upon on the paperboard of grid-like glue face after even with 5 days egg parasitoid is mixed in 4 days growing, every card contains 100 lesser calories, and every lesser calorie single deck tape-recorder goes out 1200 of honeybees.
Present technique and the Patents technology difference table of comparisons
| Item compared | Material, technology, technology that existing patented technology adopts | Material, technology, technology that this patent adopts | Difference and raising |
| The feed kind of vector | Wheat | Be with clever shell barley | Use is with clever shell barley can better keep feed humidity, is conducive to growing of gelechiid |
| Feed disinfection is processed | 60～95 ℃, 15-120 minutes, adding water behind the high temperature increased humidity and water content | Barley crosses and fills after water is eluriated dust removal In the screen frame of two sides ventilation 85～110 ℃ were descended 60～90 fens Clock, the barley water content can keep 14～20%. | The latter does not need again to add water Increase the feed water content, avoid Feed again humidification cause Fungal contamination. |
| Larva is cultivated | Need to adopt front high rear low method to keep temperature in the feed in the feeding process, in the middle of to drench feed control with disinfectant mouldy, culture period 22～30 days | Fodder Thickness is no more than 5cm, raises Support the chamber keep 28 ± 2 ℃ and 85 ± 5% relative humidity is opened weekly purple Light modulation is done indoor sterilization 2 times, training Foster 21 days namely transferable. | The latter adopts constant temperature culture favourable In biological control, exist simultaneously A receptacle is cultivated different sending out Educate the gelechiid of progress, improved The factory building utilization ratio. |
| Imago breeding and collection | Vacuum is taken out moth, the each collection 18～20 days. | Vertical adult collecting box is received moth, 2 generations of natural propagation in the collecting box, collected altogether for 3 generations, last 3 months | The latter gelechiid self is to ground Property receipts moth, Non-energy-consumption, and can In 2 generations of natural propagation gelechiid, economize The worker, save time. |
| The collection of ovum and cleaning | Supplement the nutrients, | Utilize Adult worms producting eggs cage and automatic folding ovum equipment to collect cleaner ovum, further clean gelechiid ova with the clear egg apparatus of air-flowing type and sub-sieve again and store classifiedly | The latter designed novel receipts ovum, Clear ovum equipment can significantly improve The collection of gelechiid ova and cleaning effect Rate. |
| The preservation of ovum | 3±1℃ | Numerous honeybee preserves under 4 ± 1 ℃ and 10 ± 1 ℃ respectively with ovum with ovum and numerous gelechiid | Latter's Effective Raise different usefulness Effective storage period of way ovum. Protect Depositing numerous honeybee can reach with the ovum term of validity 2 months, preserve the breeding gelechiid Can reach for 3 weeks with ovum |
| Connect the honeybee method | 1: 12 continuous numerous honeybee of Light To Dark Ratio cellular-type | 14: 10 Light To Dark Ratio connects honeybee with improved numerous beehive laying off eggs, | Latter's simulating nature circle photoperiod Numerous honeybee. |
| Storage honeybee method | Do not described 30～180 days storage periods | The diapause technology is induced in introducing, adopts Deposit (10 ± 1 ℃) with short-term With by inducing long-term storage to stagnate Educate honeybee and combine, the realization anniversary Breed. | 10 ± 1 ℃ of lower short-terms of the latter are deposited Put trichogramma and can reach for 2 weeks, logical Cross and induce the diapause technology to deposit Half a year, both combinations can be real Show numerous honeybee of anniversary, producing season Joint provides production in a large number for the field Use honeybee. |
Claims (1)
1, a kind of technology for industrially culturing trichogramma with gelechiid ova of using is characterized in that comprising following technical process:
(1), feed is selected: selecting the barley of band Ying shell for use is feed, reduces the influence that feed is subjected to the variation of ambient temperature and humidity;
(2), sterilizing pesticide: with the screen frame formula feed frame of packing into after feed (barley) the elutriation impurity elimination, in high-temperature disinfection cabinet, heat, temperature remain on 85～110 ℃ 60～90 minutes, kill insect and worm's ovum in the feed, take out back barley water content and remain on 14～20%;
(3) connect ovum parasitism and larva raising: the ratio of gelechiid ova in 1: 1000 inserted in the feed that disinfects in the larva raising chamber, feed thickness 3～4cm, the receptacle temperature remains on 28 ± 2 ℃, relative moisture remains on 85 ± 5%, and unglazed photograph is for preventing feed mold under the super-humid conditions, receptacle was opened the uviol lamp sterilization weekly 2 times each 25～35 minutes, connect 3 weeks of ovum, the feed that will contain gelechiid is put into the imago breeding frame, changes the adult collecting box in the lump over to and prepares to collect adult;
(4) raising of adult and collection: in the imago breeding chamber, temperature keeps 28 ± 2 ℃, and relative moisture keeps 80 ± 5%, and unglazed photograph, feed be from changing the adult collecting box over to eliminating 3 months, 3 generations of intermediate collection adult; The adult collecting box is a lower hopper, and the vertical slot in top is inserted room imago breeding frame, utilizes gelechiid adult geotropism to collect adult automatically, and receive every day changes gelechiid 1～2 time, changes the gelechiid adult over to the adult cage of laying eggs;
(5) collection of ovum and cleaning: at the gelechiid laying coop, temperature keeps 24 ± 2 ℃, relative moisture keeps 85 ± 5%, unglazed photograph, after the gelechiid adult was poured the cage of laying eggs into, the cage of will laying eggs was placed on the ovum collecting machine, per 2 hours, gathering machine drives the cage rotation 10 minutes of laying eggs automatically, brushes the gelechiid ova on the cage that falls to laying eggs, and the air extractor of opening simultaneously on the gathering machine is removed fine dust such as gelechiid scale; Per 24 hours, the gelechiid ova that will be dropped on the baffle plate by the special messenger collected, with the clear egg apparatus of air-flowing type and sub-sieve cleaning gelechiid ova and store classifiedly;
(6) depositing of ovum: the ovum of depositing numerous honeybee ovum respectively and being used to breed gelechiid with the refrigerator of 4 ± 1 ℃ and 10 ± 1 ℃;
(7) diffusing parasitic bee: in numerous honeybee workshop, temperature keeps 26 ± 2 ℃, relative moisture keeps 75 ± 5%, 14: 10 Light To Dark Ratios, plant honeybee and connect ovum than planting a honeybee by breeding in 1: 15,1: 8～10 propagation production are used honeybee, laying off eggs is sprinkling upon on the aluminium-plastic panel that adds mist, every plate spreads ovum 10～13g, uviol lamp down irradiation used after killing the gelechiid embryo in 30 minutes, and numerous beehive is for being improved to 13 layers of novel numerous beehive of 3 lattice of 3: 1: 3 on the numerous beehive of Morrison basis, single case day numerous honeybee 450～5,000,000, connect the honeybee time between 24～48 hours, take out the mixed pallet that changes over to of parasitic good gelechiid ova after 48 hours and grow in numerous honeybee chamber and make the ovum card after the optimum period or take other measure to handle the back and store.
(8) storage honeybee: short is to deposit under 10 ± 1 ℃ of conditions, changing refrigerator from the placement in 20 ℃ incubator of taking-up back, numerous honeybee chamber over to after half a day before egg parasitoid is deposited deposits, deposit a few days ago to stir every day and store vessel once, prevent the egg parasitoid caking, short-term is deposited with 10 days and is advisable with interior, stores the quality that use after 15～20 days will detect honeybee; Long term storage is to deposit under 3 ± 1 ℃ of conditions, adopts and induces the oophagous trichogrammae diapause, and the way of refrigeration diapause honeybee can be stored 3～6 months, generally uses later in 4～6 months in refrigeration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021293449A CN1179639C (en) | 2002-09-04 | 2002-09-04 | Technology for industrially culturing trichogramma with gelechiid ova |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021293449A CN1179639C (en) | 2002-09-04 | 2002-09-04 | Technology for industrially culturing trichogramma with gelechiid ova |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1397159A true CN1397159A (en) | 2003-02-19 |
| CN1179639C CN1179639C (en) | 2004-12-15 |
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| CNB021293449A Expired - Lifetime CN1179639C (en) | 2002-09-04 | 2002-09-04 | Technology for industrially culturing trichogramma with gelechiid ova |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101356906B (en) * | 2008-09-16 | 2010-12-15 | 广东省昆虫研究所 | Artificial propagation method and apparatus of egg parasitoids |
| CN101326902B (en) * | 2008-07-04 | 2011-05-04 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN106614396A (en) * | 2016-12-02 | 2017-05-10 | 中国农业科学院植物保护研究所 | Method for feeding sitotroga cerealella by utilizing unhusked rice |
| CN108450419A (en) * | 2018-02-26 | 2018-08-28 | 吉林省农业科学院 | A kind of Trichogramma chilonis Diapause inducement method |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
| CN111685083A (en) * | 2020-07-02 | 2020-09-22 | 吉林省农业科学院 | Method for raising wheat moths by using wheat |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933494A (en) * | 2010-08-27 | 2011-01-05 | 广东省昆虫研究所 | Moth-egg collecting machine |
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2002
- 2002-09-04 CN CNB021293449A patent/CN1179639C/en not_active Expired - Lifetime
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101326902B (en) * | 2008-07-04 | 2011-05-04 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN101356906B (en) * | 2008-09-16 | 2010-12-15 | 广东省昆虫研究所 | Artificial propagation method and apparatus of egg parasitoids |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
| CN104255660A (en) * | 2014-09-01 | 2015-01-07 | 吉林农业大学 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
| CN106614396A (en) * | 2016-12-02 | 2017-05-10 | 中国农业科学院植物保护研究所 | Method for feeding sitotroga cerealella by utilizing unhusked rice |
| CN108450419A (en) * | 2018-02-26 | 2018-08-28 | 吉林省农业科学院 | A kind of Trichogramma chilonis Diapause inducement method |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
| CN111685083A (en) * | 2020-07-02 | 2020-09-22 | 吉林省农业科学院 | Method for raising wheat moths by using wheat |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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| Publication number | Publication date |
|---|---|
| CN1179639C (en) | 2004-12-15 |
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