CN104255660A - Method for alternately storing trichogramma at intermediate and low temperature in two steps - Google Patents
Method for alternately storing trichogramma at intermediate and low temperature in two steps Download PDFInfo
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- CN104255660A CN104255660A CN201410439383.2A CN201410439383A CN104255660A CN 104255660 A CN104255660 A CN 104255660A CN 201410439383 A CN201410439383 A CN 201410439383A CN 104255660 A CN104255660 A CN 104255660A
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- trichogramma
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- oophagous trichogrammae
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000256618 Trichogramma Species 0.000 title abstract 8
- 238000003860 storage Methods 0.000 claims abstract description 18
- 241000256844 Apis mellifera Species 0.000 claims description 50
- 102000002322 Egg Proteins Human genes 0.000 claims description 17
- 108010000912 Egg Proteins Proteins 0.000 claims description 17
- 210000004681 ovum Anatomy 0.000 claims description 17
- 210000001015 abdomen Anatomy 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 21
- 241000238631 Hexapoda Species 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 5
- 238000009395 breeding Methods 0.000 abstract description 3
- 230000001488 breeding effect Effects 0.000 abstract description 3
- 230000000266 injurious effect Effects 0.000 abstract 1
- 230000002035 prolonged effect Effects 0.000 abstract 1
- 230000032669 eclosion Effects 0.000 description 7
- 241000186214 Trichogramma dendrolimi Species 0.000 description 6
- 230000017448 oviposition Effects 0.000 description 6
- 235000013601 eggs Nutrition 0.000 description 5
- 238000005520 cutting process Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000005057 refrigeration Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 241000255789 Bombyx mori Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241000346285 Ostrinia furnacalis Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000010792 warming Methods 0.000 description 3
- 241000909829 Samia ricini Species 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 210000004894 snout Anatomy 0.000 description 2
- 230000000507 anthelmentic effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000004146 energy storage Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a method for alternately storing trichogramma at intermediate and low temperature in two steps. After the trichogramma is inoculated, the trichogramma growing to the pre-pupal period is transferred to be stored for more than 70 d under the condition of the temperature of 3+/-1 DEG C and RH of 70 percent to 85 percent through intermediate-temperature storage and low-temperature storage, and field application can be carried out. According to the new method for storing the trichogramma, the storage resisting capacity of the trichogramma is improved, the storage time of the trichogramma is prolonged, manufacturers can arrange production in advance according to the trichogramma breeding plane, production capacity is enhanced without changing original plant equipment, the utilization rate of the plant equipment and the like is improved, production cost is lowered, and the control effect on injurious insects is improved.
Description
technical field:
The invention provides the method that low temperature in a kind of two steps alternately stores oophagous trichogrammae, the new method of to be a kind of with egg of oak silkworm the be trichogramma dendrolimi stored for extended periods that vector ovum is bred, belongs to the biological control of insect pests technology of agricultural technology field.
background technology:
Oophagous trichogrammae is the class ovum parasitic enemy insect that on modern agricultural biological control of insect pests, research history at most, range of application is the widest, control area is maximum, anthelminthic effect is best.The research of China to oophagous trichogrammae starts from early 1930s; After founding of New, the unit researchs such as Zhongshan University and academy of agricultural sciences of Guangdong Province are selected Eggs of Philosamia Cynthia Ricini to carry out amount reproduction oophagous trichogrammae head and are succeeded, facilitate the application of oophagous trichogrammae; 1964, academy of agricultural sciences of Shandong Province Lin Lianxin, Feng Jianguo first research and utilization tussah ovum breeding trichogramma dendrolimi of cutting open the belly succeeded, and have done the field trial of control of maize snout moth's larva.Egg of oak silkworm is compared with Eggs of Philosamia Cynthia Ricini, and egg of oak silkworm grain is large, has higher numerous honeybee efficiency, and aboundresources, cheap, is convenient to fortune storage, is widely applied in the work of artificial breeding honeybee very soon.At present, only the Northeast of China utilizes trichogramma dendrolimi control of maize snout moth's larva area to reach more than 5,000 ten thousand mu every year, occupies first place in the world.
Due to the seasonality that target pest occurs, before insect peak period, need to accumulate a large amount of oophagous trichogrammae discharge, therefore storing technology is vital link in oophagous trichogrammae factorial praluction, is to ensure that oophagous trichogrammae is flexible, the prerequisite of efficient application.In oophagous trichogrammae factorial praluction, oophagous trichogrammae stores the method for main employing deepfreeze: be developed to larva middle and later periods or prepupal period by oophagous trichogrammae, then 3 DEG C, the freezer low-temperature storage of RH60-70% is placed in, the eclosion rate average out to 80.74% when the larva middle and later periods refrigerates 30 d, reduction by 11.1% compared with normal development oophagous trichogrammae; Deformity honeybee rate reaches 15.71%, reduces increase by 8.19% compared with normal development oophagous trichogrammae; 2 months storage times eclosion rate reduces about 40%.This restriction, makes the amount reproduction of oophagous trichogrammae concentrate in before releasing one month and completes, and in the short time, a large amount of numerous honeybee just needs a large amount of factory buildings, equipment, manpower and material resources condition, and raw material can be caused to appreciate.After numerous honeybee is terminated, plant and equipment etc. leave unused again in a large number, cause the wasting of resources.And when oophagous trichogrammae application area expands year by year, existing production capacity can not be met the need of market, newly constructs the factory buildings and still needs substantial contribution to drop into.
Summary of the invention
Low temperature in a kind of two steps is the object of the present invention is to provide alternately to store the method for oophagous trichogrammae, solve the problem of the stored for extended periods of oophagous trichogrammae, oophagous trichogrammae storage time 90 more than d can be extended, break the restriction in season that oophagous trichogrammae produces, becoming seasonal production be whole year production, under the condition that factory building, equipment are constant, expand production capacity 2-3 times.
in a kind of two steps provided by the invention, low temperature alternately stores the method for oophagous trichogrammae, and its technical scheme comprises the following steps:
1. oophagous trichogrammae inoculation
Ovum of honeybee kind and fresh tussah being cut open the belly is inoculated according to the ratio of 1:25-30,25 ± 1 DEG C, darkroom meets honeybee 48 h under RH70-75% manual control condition, is developed to the larva middle and later periods under being then placed in 20-25 DEG C of temperature match curing conditions;
2. in the first step, temperature stores
The oophagous trichogrammae being developed to the larva middle and later periods is placed in 11 ± 1 DEG C, stores 25-30 d under the condition of RH70-85%, now oophagous trichogrammae is slowly developed to prepupal period under this condition;
3. second step low-temperature storage
The oophagous trichogrammae being developed to prepupal period proceeded to temperature is 3 ± 1 DEG C, store more than 70d under the condition of RH70-85%, can Field information be carried out.The longlyest reach 120 d.
good effect of the present invention is:
The new method that a kind of oophagous trichogrammae stores is proposed, improve resistance to energy storage power and the storage time of oophagous trichogrammae, manufacturer can produce according to numerous honeybee plan schedule ahead, production capacity is strengthened under the condition that original plant and equipment is constant, improve the availabilitys such as plant and equipment, reduce production cost, improve the control effects to insect.
Specific embodiments
For the ease of understanding the present invention, especially exemplified by following examples.Its effect is understood to be explaination of the present invention but not to any type of restriction of the present invention.
embodiment 1:
Be that trichogramma dendrolimi produced by raw material with tussah cocoon, alternately store by low temperature in two steps, early July Field information to prevent and treat generation corn borer be example:
1. the production of honeybee kind:
Start warming cocoons moth appearance mid-December, on January about 10 got ovum of cutting open the belly after going out moth, then honeybee is connect in 1:35-40 ratio, 23-25 DEG C, after darkroom meets honeybee 24 h under RH70-75% condition, remove honeybee kind, be developed to the larva middle and later periods under proceeding to 20-25 DEG C of alternating temperature, RH70-75%, L16:D8 condition, refrigerate for subsequent use.
2. the production of host's ovum
On February about 25 started to hang cocoon, 18-23 DEG C, heat under the condition of RH60-65%, after after going out moth on March about 17, collecting female moth, cut open the belly and get ovum, clean, extruding green ovum, 1 ‰ bromogeramines sterilization 10min, dry for subsequent use.
3. the production of oophagous trichogrammae
Connect honeybee on March about 17 to start to produce, when honeybee kind goes out honeybee 20-25%, connect honeybee in 1:25-30 ratio, 25 ± 1 DEG C, after darkroom meets honeybee 48 h under RH70-75% condition, remove honeybee kind, be developed to the larva middle and later periods under proceeding to 20-25 DEG C of alternating temperature, RH70-75%, L16:D8 condition, i.e. on March about 20.
4. in the first step, temperature stores
The oophagous trichogrammae being developed to the larva middle and later periods is placed in 10 DEG C, stores 30 d under the condition of RH70-85%, i.e. on April about 20, now oophagous trichogrammae is developed to prepupal period.
5. second step low-temperature storage
The oophagous trichogrammae being developed to prepupal period proceeded to temperature is 3 ± 1 DEG C, store 70d under the condition of RH70-85%, namely take out at the beginning of 7 months and heat, fabrication, field adaptability.Store oophagous trichogrammae in this way, eclosion rate can reach 81.25%, and plumage leaves over honeybee 8.25%, and single honeybee egg laying amount reaches 212.43(), folding wing rate 10.89%.For Field information in annual July, according to said method store and can start to hang cocoon in annual February and produce, 70d can be shifted to an earlier date than conventional storage method and produce.
embodiment 2
Be that trichogramma dendrolimi produced by raw material with tussah cocoon, mid-July Field information to prevent and treat generation corn borer be example:
2. the production of honeybee kind:
Late December starts warming cocoons moth appearance, on January about 20 got ovum of cutting open the belly after going out moth, then honeybee is connect in 1:35-40 ratio, 23-25 DEG C, after darkroom meets honeybee 24 h under RH70-75% condition, remove honeybee kind, be developed to the larva middle and later periods under proceeding to 20-25 DEG C of alternating temperature, RH70-75%, L16:D8 condition, refrigerate for subsequent use.
2. the production of host's ovum
On March about 5 started to hang cocoon, 18-23 DEG C, heat under the condition of RH60-65%, after after going out moth on March about 25, collecting female moth, cut open the belly and get ovum, clean, extruding green ovum, 1 ‰ bromogeramines sterilization 10min, dry for subsequent use.
3. the production of oophagous trichogrammae
Connect honeybee on March about 25 to start to produce, when honeybee kind goes out honeybee 20-25%, connect honeybee in 1:25-30 ratio, 25 ± 1 DEG C, after darkroom meets honeybee 48 h under RH70-75% condition, remove honeybee kind, be developed to the larva middle and later periods under proceeding to 20-25 DEG C of alternating temperature, RH70-75%, L16:D8 condition, i.e. on March about 28.
4. in the first step, temperature stores
The oophagous trichogrammae being developed to the larva middle and later periods is placed in 11 DEG C, stores 30 d under the condition of RH70-85%, i.e. on April about 20, now oophagous trichogrammae is developed to prepupal period.
5. second step low-temperature storage
The oophagous trichogrammae being developed to prepupal period proceeded to temperature is 3 ± 1 DEG C, store 80d under the condition of RH70-85%, namely take out at the beginning of 7 months and heat, fabrication, field adaptability.Store oophagous trichogrammae in this way, eclosion rate can reach 80.30%, and plumage leaves over honeybee 8.56%, and single honeybee egg laying amount reaches 211.56(), folding wing rate 11.89%.For Field information annual mid-July, according to said method store and can start to hang cocoon produce at the beginning of annual 3 months, 80d can be shifted to an earlier date than conventional storage method and produce.
embodiment 3
Be that trichogramma dendrolimi produced by raw material with tussah cocoon, August Field information prevent and treat two generation corn borer be example:
1, the production of honeybee kind:
Start warming cocoons moth appearance mid-January, on February about 8 got ovum of cutting open the belly after going out moth, then honeybee is connect in 1:35-40 ratio, 23-25 DEG C, after darkroom meets honeybee 24 h under RH70-75% condition, remove honeybee kind, be developed to the larva middle and later periods under proceeding to 20-25 DEG C of alternating temperature, RH70-75%, L16:D8 condition, refrigerate for subsequent use.
2. the production of host's ovum
On March about 26 started to hang cocoon, 18-23 DEG C, heat under the condition of RH60-65%, after after going out moth on April about 15, collecting female moth, cut open the belly and get ovum, clean, extruding green ovum, 1 ‰ bromogeramines sterilization 10min, dry for subsequent use.
3. the production of oophagous trichogrammae
Connect honeybee on April about 15 to start to produce, when honeybee kind goes out honeybee 20-25%, connect honeybee in 1:25-30 ratio, 25 ± 1 DEG C, after darkroom meets honeybee 48 h under RH70-75% condition, remove honeybee kind, be developed to the larva middle and later periods under proceeding to 20-25 DEG C of alternating temperature, RH70-75%, L16:D8 condition, i.e. on April about 18.
4. in the first step, temperature stores
The oophagous trichogrammae being developed to the larva middle and later periods is placed in 12 DEG C, stores 30 d under the condition of RH70-85%, i.e. on May about 18, now oophagous trichogrammae is developed to prepupal period.
5. second step low-temperature storage
The oophagous trichogrammae being developed to prepupal period proceeded to temperature is 3 ± 1 DEG C, store 90d under the condition of RH70-85%, namely take out mid-August and heat, fabrication, field adaptability.
Store oophagous trichogrammae in this way, eclosion rate can reach 82.14%, leaves over honeybee 7.56%, and single honeybee egg laying amount reaches 210.23(), folding wing rate 11.12%.For Field information in annual August, according to said method store and can start to hang cocoon in annual March and produce, 90 d can be shifted to an earlier date than conventional storage method and produce.
good effect place of the present invention is shown by following contrast experiment:
By low-temperature storage 70-90d in two steps, store compared with refrigeration 30 d with Conventional cryogenic, eclosion rate improves 0.51%, leave over honeybee rate reduces 1.9%, single honeybee egg laying amount reaches 211.41(), it is 2.1 times of the mono-honeybee egg laying amount 99.83 of refrigeration 30 d, folding wing rate 11.30%, rolls over wing rate 15.71% lower than refrigeration 30d.
The biological character that table 1 stores oophagous trichogrammae compares
| Process | Eclosion rate/% | Leave over honeybee rate/% | Single honeybee egg laying amount/ | Folding wing rate/rate |
| Embodiment 1 | 81.25 | 8.25 | 212.43 | 10.89 |
| Embodiment 2 | 80.30 | 8.56 | 211.56 | 11.89 |
| Embodiment 3 | 82.14 | 7.56 | 210.23 | 11.12 |
| On average | 81.23 | 8.12 | 211.41 | 11.30 |
| Conventional refrigeration 30d | 80.74 | 10.02 | 99.83 | 15.71 |
Claims (1)
1. in two steps, low temperature alternately stores a method for oophagous trichogrammae, it is characterized in that comprising the following steps:
1) oophagous trichogrammae inoculation
Ovum of honeybee kind and fresh tussah being cut open the belly is inoculated according to the ratio of 1:25-30,25 ± 1 DEG C, darkroom meets honeybee 48 h under RH70-75% manual control condition, is developed to the larva middle and later periods under being then placed in 20-25 DEG C of temperature match curing conditions;
2) in the first step, temperature stores
The oophagous trichogrammae being developed to the larva middle and later periods is placed in 11 ± 1 DEG C, stores 25-30 d under the condition of RH70-85%, now oophagous trichogrammae is slowly developed to prepupal period under this condition;
3) second step low-temperature storage
The oophagous trichogrammae being developed to prepupal period proceeded to temperature is 3 ± 1 DEG C, store more than 70d under the condition of RH70-85%, can Field information be carried out.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410439383.2A CN104255660A (en) | 2014-09-01 | 2014-09-01 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
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| CN201410439383.2A CN104255660A (en) | 2014-09-01 | 2014-09-01 | Method for alternately storing trichogramma at intermediate and low temperature in two steps |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109156437A (en) * | 2018-08-01 | 2019-01-08 | 广东省生物资源应用研究所 | A kind of method of In Vitro Rearing of Trichogramma low-temperature storage |
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| CN1397159A (en) * | 2002-09-04 | 2003-02-19 | 衡水田益生防有限责任公司 | Technology for industrially culturing trichogramma with gelechiid ova |
| CN1647626A (en) * | 2005-01-26 | 2005-08-03 | 北京市农林科学院 | Artificial breeding and producing method for trichogramma |
| CN101326902A (en) * | 2008-07-04 | 2008-12-24 | 衡水田益生防有限责任公司 | Method for inducing diapause of Trichogramma ostriniae by constant temperature and storage method |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
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2014
- 2014-09-01 CN CN201410439383.2A patent/CN104255660A/en active Pending
Patent Citations (4)
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| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109156437A (en) * | 2018-08-01 | 2019-01-08 | 广东省生物资源应用研究所 | A kind of method of In Vitro Rearing of Trichogramma low-temperature storage |
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