CN111316981B - Method for refrigerating adult arma chinensis - Google Patents
Method for refrigerating adult arma chinensis Download PDFInfo
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- CN111316981B CN111316981B CN202010201120.3A CN202010201120A CN111316981B CN 111316981 B CN111316981 B CN 111316981B CN 202010201120 A CN202010201120 A CN 202010201120A CN 111316981 B CN111316981 B CN 111316981B
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- chinensis
- adult
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- arma chinensis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a method for refrigerating adult arma chinensis, which comprises the steps of considering the low temperature resistance characteristic of adult arma chinensis, carrying out short-term low-temperature adaptation on adult arma chinensis in a refrigeration house for a certain eclosion time, and then putting the adult arma chinensis in the refrigeration house with lower temperature for medium-term and long-term storage; when the adult arma chinensis needs to be used, the arma chinensis is moved out of the refrigeration house and is gradually heated to the normal temperature. Thereby realizing the medium-long term storage of the adult arma chinensis and being used for preventing and controlling pests at any time.
Description
Technical Field
The invention relates to the technical field of artificial feeding of insects, in particular to a method for refrigerating adult arma chinensis.
Background
The arma bug belongs to the euschistosoideae of the hemiptera, is an excellent predatory natural enemy insect, can predate various pests of lepidoptera, coleopteran and hemiptera, and has an important application prospect in the biological control of agricultural and forestry pests.
In China, the arma chinensis has been widely applied to biological control of lepidoptera pests on tobacco and also applied to garden trees and vegetable crops in the north. With the increase of the environmental protection attention of the country, the pesticide composition can be widely applied to pest control of plants such as tea leaves, fruit trees, garden greens and protected vegetables in short time. At present, a plurality of arma bugs propagation bases and factories exist in China, and the total quantity of arma bugs produced per year exceeds one million.
Due to the fact that the breadth of China is wide, the occurrence time of agricultural and forestry pests is different in various places, the damage levels are different every year, and the demand for the arma bugs is not equal. A large amount of lugworm bugs are needed urgently when pests are in a big occurrence, but the lugworm bugs in proper age are insufficient in quantity, cannot effectively exert the pest control effect, and only depend on chemical pesticides again; when a large amount of arma bug is bred, the pest occurrence amount often does not reach the economic hazard level, arma bug release is not needed, the labor cost and the material cost for breeding are increased, the price of arma bug is further increased, the cost for biological prevention and control is increased, and the enthusiasm of producers and users is reduced.
Although the incubation time of the lugworm eggs is delayed through refrigeration in the prior art, the lugworm eggs can be stored only for a short time, the incubation rate of the lugworm eggs is extremely low after 26 days, the incubation period is prolonged, and 15-22 days are needed from the development of the eggs to the insect state capable of being used for preventing and controlling pests, so that natural enemy insect products cannot be supplied in time. In addition, some of the existing methods for storing arma chinensis are complex and tedious in operation link, and food and water are required to be supplemented in the refrigeration process, host plants are required to be replaced, and the like. Furthermore, arma bugs are placed in the cups during refrigeration and cannot be stored more at one time. After the method is adopted for cold storage at 5 ℃ and 10 ℃ for 30 days respectively, the survival rate of the adults is only 33.65 percent and 45.21 percent, if the cold storage period is prolonged, the survival rate can be further reduced, the actual production requirements cannot be met in the cold storage period and the survival rate, and the indirect cost is increased; although the survival rate is higher at 15 ℃ and 20 ℃, the actual production is not very meaningful because the arma chinensis does not sleep and needs to be eaten at the temperature, thereby increasing the cost of artificial feeding.
Therefore, the method for simply and conveniently storing the adult arma chinensis by refrigeration for a medium-long period is designed, and the high survival rate can be ensured, so that the method has important significance for production practice.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention aims to: the simple method for refrigerating adult arma chinensis can efficiently realize medium-and-long-term refrigerating storage of adult arma chinensis and can obtain higher survival rate.
The purpose of the invention is realized by the following technical scheme: the method comprises the following steps:
(1) selecting adult arma chinensis which is eclosion for 20 to 100 hours in the insect breeding room;
(2) putting the adult arma chinensis selected in the step (1) in a refrigeration house at the temperature of 10-15 ℃ for 48-96 hours;
(3) placing the adult arma chinensis in the step (2) into a storage box, placing the adult arma chinensis in a refrigeration house at the temperature of 3-7 ℃ for storage, placing wet cotton on the storage box for moisture preservation, and leaving vent holes or a gauze on a cover of the storage box, wherein the wet cotton is placed on the gauze;
(4) and (4) taking out the storage box in the step (3), placing the storage box in a refrigeration house with the temperature of 10-15 ℃ for 2-6 hours, and then placing the storage box in an insect breeding chamber for feeding for 12-24 hours.
Preferably, 1000 arma chinensis adults are placed in the storage box in the step (3) at most.
Preferably, shredded paper, corrugated paper, newspaper or toilet paper is placed in the storage box in the step (3).
Preferably, the temperature in the insect rearing chamber in the step (1) and the step (4) is 25 ℃ to 28 ℃.
Preferably, the arma chinensis adults in the step (3) are placed in the storage box for 400 days of emergence within 24 hours.
Preferably, the adult arma chinensis placed in the storage box within 24 hours after 48 hours is moved to a cold storage at 12 ℃ for feeding.
Preferably, after 48 hours, the Armoracia chinensis imagoes in the refrigerator at 12 ℃ are transferred to the refrigerator at 5 ℃ for storage.
Preferably, the storage box in the step (3) is a plastic box, a paper box, a metal box or a wood box.
Preferably, the storage box is a plastic box, and the length of the plastic box is 10 cm to 35 cm, the width of the plastic box is 10 cm to 28 cm, and the height of the plastic box is 4 cm to 12 cm.
Preferably, the storage box is a plastic box, and the length and the width of the storage box are both 12 centimeters and the height of the storage box is 5.5 centimeters.
Compared with the prior art, the invention has the following advantages and effects:
the method for refrigerating adult arma chinensis in the invention considers the low temperature resistance characteristic of adult arma chinensis, carries out short-term low temperature adaptation on adult arma chinensis in a refrigeration house for a certain eclosion time, then puts the adult arma chinensis in the refrigeration house with lower temperature for medium and long term storage, and when the adult arma chinensis needs to be used, moves out from the refrigeration house and gradually heats up to normal temperature. Thereby realizing the medium-long term storage of the adult arma chinensis and being used for preventing and controlling pests at any time.
Drawings
FIG. 1 is the survival rate of adult arma bug in the first embodiment of the method for cold storage of adult arma bug of the present invention;
FIG. 2 is the survival rate of adult arma bug in the second embodiment of the method for cold storage of adult arma bug of the present invention;
fig. 3 shows survival rate of adult arma bug in third embodiment of the method for cold storage of adult arma bug of the present invention.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
As shown in fig. 1 to fig. 3, the present invention is a method for cold storage of adult arma bug, comprising the following steps:
(1) selecting adult arma chinensis which is eclosion for 20 to 100 hours in the insect breeding room;
(2) placing the adult arma chinensis in a refrigeration house at the temperature of 10-15 ℃ for low-temperature adaptation for 48-96 hours;
(3) placing adult arma chinensis in a storage box, placing the storage box in a refrigeration house at 3-7 ℃, not feeding food and water, and placing wet cotton in the storage box for moisturizing;
(4) taking out the storage box, putting the storage box in a refrigeration house with the temperature of 10-15 ℃ to adapt for 2-6 hours, then putting the storage box in an insect breeding chamber to feed for 12-24 hours, and then using the storage box for breeding or controlling pests.
Wherein, 1000 arma chinensis imagoes are placed in the storage box in the step (3) at most. In addition, shredded paper scraps, corrugated paper, newspaper or toilet paper are placed in the storage box in the step (3) in advance, and a vent hole or a gauze is reserved on a cover of the storage box. The shredded paper is placed in the storage box, the volume of the storage box is one fourth to one half, and the number of the newspapers is two to four. At the same time, wet cotton is placed on the screen.
And (4) when the step (3) is carried out, labeling is required to be attached to the plastic box, and the refrigerating time and the number of adult arma bug are recorded.
The temperature in the insect chamber in step (1) and step (4) is 25 ℃ to 28 ℃, preferably 26 ℃.
The storage box in the step (3) is preferably a plastic box, a paper box, a metal box or a wood box. In this embodiment, the storage box is a plastic box, and the length of the plastic box is 10 cm to 35 cm, the width of the plastic box is 10 cm to 28 cm, and the height of the plastic box is 4 cm to 12 cm.
Based on field observation, the arma bugs live through winter in the form of adults in northeast and north China, the arma bugs adults are relatively low-temperature resistant, hide under eaves, dead branches and leaves, bark gaps and other wind shelters, and are not eaten or moved in the whole winter and are in a dormant state. Therefore, considering the low temperature resistance of adult arma bug, adult arma bug in a certain emergence time is fed with enough food, then is subjected to low temperature adaptation in a refrigeration house, and finally is stored in the refrigeration house at lower temperature.
Under the condition of adopting the method, the method for refrigerating adult arma bug preservation is respectively tested and analyzed from the following three embodiments, and the conclusion of the survival rate of arma bug in the corresponding embodiments is obtained.
Example one
Placing 200 adult arma chinensis with emergence time within 48 hours in the plastic box in the step (3), feeding the adult arma chinensis in a insect-raising room at the temperature of 26 ℃ and feeding sufficient tussah pupas and water; after 48 hours, 200 Armoracia chinensis imagoes are transferred to a refrigeration house with the temperature of 12 ℃ for low-temperature adaptation; after 48 hours, move to a freezer at 5 ℃, place arma bug adults in one plastic box every 50 th, for a total of four boxes. Wherein, the length and the width of the plastic box are both 12 cm, and the height is 5.5 cm; finally, a box of 50 arma chinensis adults was taken out after 30 days, 45 days, 60 days and 90 days, respectively, and their survival rates were examined.
As shown in fig. 1, the average survival rates of adult arma chinensis obtained in the first embodiment at different refrigeration time are: 92.0%, 87.0%, 72.7%, 62.0%.
Example two
Placing 400 jujubes with emergence time within 24 hours in the plastic box in the step (3), feeding the adult arma chinensis in a insect-rearing room at the temperature of 26 ℃, and feeding sufficient tussah pupas and water; after 48 hours, the 400 arma chinensis imagoes are moved to a refrigeration house with the temperature of 12 ℃ for low-temperature adaptation; after 48 hours, move to a freezer at 5 ℃ and store adult arma bug every 100 th in one plastic box for a total of four boxes. Wherein, the length and the width of the plastic box are both 12 cm, and the height is 5.5 cm; finally, a box of 100 adult arma chinensis was taken out after 30 days, 45 days, 60 days and 90 days, respectively, and their survival rates were examined.
As shown in fig. 2, the average survival rates of the adult arma bug obtained in example two at different refrigeration times were: 98.7%, 97.0%, 91.0%, 71.7%.
EXAMPLE III
Placing 1000 adult arma chinensis with eclosion time within 48 hours in the plastic box in the step (3), feeding in a insect breeding room with the temperature of 26 ℃, and feeding sufficient tussah pupas and water; after 48 hours, the 1000 fleshy bugs are transferred to a refrigerator at 12 ℃ for low-temperature adaptation; after 48 hours, move to 5 ℃ freezer, store arma bug adults every 250 heads in one plastic box for four boxes. Wherein the plastic box is 32 cm long, 25 cm wide and 9 cm high; finally, a box of 250 arma bug adults was taken out after 30 days, 45 days, 60 days and 90 days, respectively, and their survival rates were examined.
As shown in fig. 3, the average survival rates of adult arma chinensis obtained in example three at different refrigeration times were: 96.7%, 83.7%, 66.7%, 59.0%.
In the first to third examples, the survival rate of adult arma chinensis is equal to 100% of the number of heads of surviving bugs/total number of heads of removed bugs. Also, each example was tested in triplicate and survival averaged. The survival rate of adult arma bug refrigerated and stored by the method of the second embodiment is the highest, the survival rate after refrigerating for 60 days is up to more than 90%, and the survival rate after refrigerating for 90 days (about 3 months) is still more than 70%. By adopting the method for refrigerating adult arma chinensis, the survival rate of refrigerated arma chinensis is far greater than that of the prior art. Can be greatly expanded when the arma chinensis needs a low season, and then the technical method is adopted for refrigeration storage, and the arma chinensis is taken out when needed and released in time, so as to achieve the effect of quick response to biological control of pests.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (9)
1. A method for refrigerating and storing Armoracia chinensis imagoes is characterized by comprising the following steps:
(1) selecting adult arma chinensis which is eclosion for 24-48 hours in a pest breeding room, and feeding the adult arma chinensis in the pest breeding room at the temperature of 26 ℃;
(2) putting the adult arma chinensis selected in the step (1) in a refrigeration house at the temperature of 12 ℃ for 48 hours;
(3) placing the adult arma chinensis in the step (2) into a storage box, placing the adult arma chinensis in a refrigeration house at 5 ℃ for storage, placing wet cotton on the storage box for moisture preservation, and leaving vent holes or a gauze on a cover of the storage box, wherein the wet cotton is placed on the gauze.
2. The method for cold storage of adult arma chinensis as claimed in claim 1, wherein the storage box in step (3) contains at most 1000 adult arma chinensis.
3. The method for cold storage of adult armadilla chinensis as claimed in claim 2, wherein shredded paper, corrugated paper, newspaper or toilet paper is placed in the storage box in step (3).
4. The method for refrigerating Armoracia chinensis adults for preservation according to claim 1, wherein the Armoracia chinensis adults in step (1) with emergence of 400 heads for 24 hours are selected, placed in an insect-rearing room, and reared in the insect-rearing room at 26 ℃.
5. The method for the refrigerated storage of adult arma chinensis as claimed in claim 4, wherein adult arma chinensis in the insect rearing room is moved to a freezer of 12 ℃ for acclimatization after 48 hours.
6. The method for cold storage of adult arma chinensis as claimed in claim 5, wherein the adult arma chinensis 400 in the refrigerator at 12 ℃ is transferred into a storage box after 48 hours, and then stored in the refrigerator at 5 ℃.
7. The method for the refrigerated storage of adult armadilla chinensis as claimed in claim 1, wherein the storage box in step (3) is a plastic box, a paper box, a metal box or a wooden box.
8. The method of refrigerated storage of adult armadilla chinensis as recited in claim 7, wherein said storage box is a plastic box and said plastic box has a length of 10 cm to 35 cm, a width of 10 cm to 28 cm and a height of 4 cm to 12 cm.
9. The method of claim 6, wherein the storage box is a plastic box having a length and width of 12 cm and a height of 5.5 cm.
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| CN202010201120.3A CN111316981B (en) | 2020-03-20 | 2020-03-20 | Method for refrigerating adult arma chinensis |
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| CN202010201120.3A CN111316981B (en) | 2020-03-20 | 2020-03-20 | Method for refrigerating adult arma chinensis |
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| CN111316981B true CN111316981B (en) | 2022-08-26 |
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| CN112106734B (en) * | 2020-09-28 | 2023-04-28 | 贵州省烟草公司黔东南州公司 | Refrigeration diapause method for armyworms |
| CN114521537B (en) * | 2022-02-17 | 2023-04-07 | 中国农业科学院植物保护研究所 | Lugworm diapause induction method and long-term storage method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN209097414U (en) * | 2018-12-04 | 2019-07-12 | 贵州省烟草公司遵义市公司绥阳县分公司 | A kind of Candle-sticks stinkbug transport case |
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| CN109548754B (en) * | 2018-12-06 | 2022-04-29 | 甘肃农业大学 | Low-temperature storage method of adult ladybug |
| CN109757441B (en) * | 2019-03-13 | 2021-05-18 | 中国农业科学院植物保护研究所 | Method for storing arma chinensis |
| CN110463665A (en) * | 2019-07-31 | 2019-11-19 | 黄勇 | A kind of with yellow meal worm is the Candle-sticks stinkbug mating system of host |
| CN110447602B (en) * | 2019-08-14 | 2021-11-30 | 广东省农业科学院植物保护研究所 | Feed and domestication box are fed to resistant low temperature of Indian Muntjac ant |
| CN110476901A (en) * | 2019-09-17 | 2019-11-22 | 中国农业科学院植物保护研究所 | A method of extending Candle-sticks stinkbug ovum shelf life |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN209097414U (en) * | 2018-12-04 | 2019-07-12 | 贵州省烟草公司遵义市公司绥阳县分公司 | A kind of Candle-sticks stinkbug transport case |
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Application publication date: 20200623 Assignee: GUANGZHOU YUEFENG BIOLOGICAL CONTROL TECHNOLOGY Co.,Ltd. Assignor: PLANT PROTECTION Research Institute GUANGDONG ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2022440000296 Denomination of invention: A method for cold storage of adults of stinkbug Granted publication date: 20220826 License type: Common License Record date: 20221206 |
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