KR101935104B1 - Method for self-production Hypoaspis aculeifer and pest control method using the same - Google Patents

Abstract

The present invention relates to a method for self-production of Hypoaspis aculeifer , a predatory mite, and a method for controlling pests using the same, and can efficiently produce a mite mite at low cost and provide a great effect on pest control. In consideration of human health problems and environmental problems, it will be useful to control pests that do not use pesticides or greatly reduce the use of pesticides, but interfere with the growth of crops.

Description

Technical Field [0001] The present invention relates to a method for self-production of paprika and a pest control method using the same,

The present invention relates to a method for self-production of a spore mite, a predatory mite, and a pest control method using the same.

Pests of crops are a major factor in the loss of important crops in the world. Recently, the agriculture (agricultural) form has been emphasizing the control of the production period and the production of high quality agricultural products by growing in plastic greenhouse. Therefore, the cultivation area of vinyl house has increased greatly and the need for pest management It is gradually increasing.

Among the insect species that occur mainly in the crops of Korea, 11 kinds such as slugs occurring in radish and Chinese cabbage belonging to the cruciferous family, 10 kinds of cotton aphids such as cucumber, melon, watermelon, (See Table 1 below), including nine species such as peach black aphid which occur in branches such as red pepper, green pepper, and red pepper, and worms which occur in lilies such as onion and onion (see Table 1 below) It is a general object of control.

crops Pests (dark characters: important pests) Radish, Chinese cabbage Mosquito larvae, cabbage leafhopper, cabbage moth, cabbage moth, cotton aphid, peach black aphid, bark aphid, cabbage flour aphid, Cucumber, melon, watermelon, pumpkin, melon Cotton aphid, greenhouse powder, flower yellow thrips, cucumber thrips, american leaf jellyfish, spotted mite, small moths moth , moths moth , moths nymphs, rootstock nematode, Pepper, bell pepper, colored sweet pepper Peach black aphid, tobacco moth, cotton aphid, spotted mite, greenhouse powder, flower yellow thrips, cucumber thripworm, rootstock nematode, tea dust mite Eggplant, tomatoes, potatoes Ongal powder, Yellow thripworm, American leaf jellyfish, Peach black aphid, Potato beard aphid, Scarab bee aphid, Tea dust mite Wave, onion Mosquitoes, mosquito bugs, mosquito nets, tobacco mosquito nets, parakeets, parrot flies, root mite, highland flies, Top Peach black aphid, potato beard aphid, boll beard aphid, field slug, slug Strawberry Aphididae, Strawberry mite, Strawberry nematode, Root-nematode, Root-nematode, Spotted nematode, Spotted mite, Tea mite, Flower yellow thrips, Cotton aphid, Peach black aphid, Strawberry root aphid, Leaf perilla Perilla aphid, perilla leafy moth, moth mite, greenhouse powder, flower yellow thrips, spotted mite

One of the effective ways to treat these pests is to use pesticides. Pesticides have the advantages of fast and accurate insect pest control, , The destruction of the surrounding environment, the development of pest resistance, and the emergence of potential pests.

As a result, new pest control measures have become necessary, and one of them is predator mite as a biological control agent. These natural enemies were produced by natural enemies and sold to farmhouses. However, as the natural enemies of the natural enemies have disappeared, as shown in Table 2 below, almost all natural enemies, It is sold.

division Manufacturer name unit standard Consumers Overseas production



















Slippery bottle 500 grains / bottle 44,000 Miles Mite
bottle 10,000 grains / bottle 28,000 bulk 12,500 / bulk 300,000 Mediterranean sea turtle

pack / box 500pack / box 350,000 pack / box 100pack / box 80,000 bottle 25,000 grains / bottle 65,000 Chilean mite bottle 2,000 bottles / bottle 33,000 Torii mackerel bottle 500 grains / bottle 80,000 Desert caterpillar pack / box 500pack / box 680,000 bottle 2,000 bottles / bottle 25,000 Cucumber mite bottle 25,000 grains / bottle 40,000 Greenhouse powder Card 5,000 cards / card 50,000 Sulfur Card 5,000 cards / card 50,000 Abby Honeybee bottle 250 grains / bottle 40,000 Collemani Honeybee bottle 500 grains / bottle 22,000 Peach humpback bottle 500 grains / bottle 30,000 Gulp bottle 250 grains / bottle 48,000 Aphid fly tray 1,000 grains / tray 44,000 Pod ladybug bottle 500 grains / bottle 220,000 Moth
bottle 1,000 grains / bottle 60,000 bulk 10,000 grains / bulk 300,000 Domestic production
Banker plant Flowerpot 100 mummy / flower pots 35,000 Tobacco blind bottle 250 grains / bottle 60,000

Especially, because natural enemies are living creatures, there is a problem of quality degradation due to prolonged delivery due to lack of vitality after a certain period of time after packaging.

Korean Patent No. 10-1549254 Korean Patent No. 10-1469056

Accordingly, the present inventors have made efforts to obtain technologies for directly utilizing necessary nematodes to produce nematodes that are directly needed in farmhouses in order to supply high quality nemesis, and as a result, they have simplified the complicated and complicated production process for mass breeding Everyone has completed the present invention by developing a technique for self-production of the bryophyta.

It is an object of the present invention to provide a self-production method of the mulberry tree mulberry.

Another object of the present invention is to provide a composition for controlling insects, which comprises the above-mentioned method.

It is another object of the present invention to provide a method for controlling pests of crops using the composition including the mulberry tree mite produced by the above method.

In order to solve the above object,

1) breeding the breeding box by inserting the outer box and the built-in box by inserting the prey mite Tyrophagus putrescentiae and the breeding medium into the inner box; And

2) placing the hinoki mites and rearing media reared in step 1) in a new built-in box and then covering the cover of the outer box with Hypoaspisaculeifer to feed on the mildew mite mite; It provides a self-production method of mites. In this method, the breeding box is divided into an external box and a built-in box, a breeding medium is put in a built-in box, water is put in an external box to maintain the moisture of the medium, and the mousse is produced in a simple manner .

In one embodiment of the present invention, when the exterior box and the built-in box are stacked, the height of the built-in box does not touch the cover of the exterior box, and the exterior box has a cover to fill the exterior box with water, It can be closing.

In one embodiment of the present invention, the breeding medium of step 1) may include rice bran, wheat or grain flour, cereal by-products and dry yeast, and the breeding medium May include rice hull or filler, sawdust and hinoki mite cultivated in step 1) above.

The present invention also provides a composition for controlling insects, which comprises the above-mentioned method.

The present invention also provides a method for controlling pests of a crop using the composition.

In one embodiment of the present invention, the insect pests may be at least one species selected from the group consisting of an insect pests, a rootstock, a rootstock, a small rootstock and a slug.

In one embodiment of the present invention, the crop is selected from the group consisting of cucumber, watermelon, lettuce, Poinsettia, onion, onion, paprika, strawberry, melon, melon, pumpkin, pepper, bell pepper, And perilla seeds.

The production method of the present invention of the present invention can provide a great effect to the pest control by utilizing the low cost and high quality natural enemies through the self production of the natural enemy in a proper time and accordingly, The use of pesticides or the use of pesticides to reduce the use of pesticides significantly reduces the effect of the control of the pest is excellent, and pesticide-free farmers and organic farmers to increase the value of the use of agricultural income, thereby increasing the pesticide And to reduce the production cost, to secure the safety of agricultural products, to build healthy agricultural environment, and to contribute to the development and activation of agriculture sector.

Fig. 1 is an illustrative photograph of a breeding box used by a natural enemy company.
2 is an exemplary photograph of a built-in box of a breeding box used in the present invention.
3 is an exemplary photograph of an outer box of a breeding box used in the present invention.
Fig. 4 is an exemplary photograph in which the built-in and external boxes used in the present invention are superimposed.
Fig. 5 is an illustrative photograph of rice bran, dry yeast and rice hull used in the present invention.
6 is a photograph of a breeding tool used in the present invention.
Figure 7 is an exemplary photograph of an actual breeding box used in the present invention: a. Bamboo flour mite, actual breeding box, b. Actual breeding boxes of the present invention.
FIG. 8 is a graph showing the moisture content of the medium used in the cultivation of alluvial rhizome of the present invention.
FIG. 9 is a graph showing the density of gibberellicidal mites raised by the method of raising gibberellic acacia of the present invention.
FIG. 10 is a graph showing the density increase rate of athlete's thorn mite according to the present invention.

Hereinafter, the present invention will be described in detail.

The present invention relates to: 1) breeding a breeding box by inserting an external box and a built-in box into a built-in box, inserting a prey mite Tyrophagus putrescentiae and a breeding medium to cover the outer box; And

2) placing the hinoki mites and rearing media reared in step 1) in a new built-in box and then covering the cover of the outer box with Hypoaspisaculeifer to feed on the mildew mite mite; It is about self-production method of mite.

The present invention relates to the field of environmentally friendly biocontrol. Specifically, the present invention relates to the use of predator mites as biocontrol agents for reducing damage to crops by insect pests. In particular, the present invention relates to a novel method for self-producing and feeding predatory mites, a filler composition for nematocidal raising, a composition for raising nematodes, a container for breeding, a composition for pest control, To a method of controlling pests.

It is a domesticated nematode that is widely domesticated in the soil due to domestic native enemy.

Table 2 is a list of the major natural enemies sold in Korea and the price of the product (15 ~ 16 years old). As shown in Table 2, almost all natural enemies, which are produced in Korea, are produced by foreign companies and imported and sold as natural enemies. .

Therefore, the increase in the cost of using natural enemies due to the importation of foreign companies, and especially the natural enemies are living creatures, there is a problem of deteriorating quality of natural enemies due to long delivery due to lack of vitality after a certain period of time after product packaging. Therefore, it is most effective to produce the natural enemies directly from the farmers and to utilize them directly at the necessary time in order to supply the high quality natural enemies.

The inventors of the present invention have studied to obtain a technique for directly utilizing necessary nematodes to produce high quality natural enemies directly at the farmhouse, and as a result, they have simplified the complicated and complicated production process for mass breeding in natural enemies, The present invention has been accomplished by establishing a technique for self-production of bryophyta mite for breeding.

In one embodiment of the present invention, when the exterior box and the built-in box are stacked, the height of the built-in box does not touch the cover of the exterior box, and the exterior box has a cover to fill the exterior box with water, It can be closing.

In one embodiment of the present invention, the breeding medium of step 1) may include rice bran, wheat or grain flour, cereal by-products and dry yeast, and the breeding medium May include rice hull or filler, sawdust and hinoki mite cultivated in step 1) above.

The humidity of the rearing media is the most important factor in the indoor rearing environment, and water is laid on the outer box to keep the moisture in the medium in the built-in box so that the humidity of the medium can be easily maintained. Also, it is possible to breed without any special tools or tools because it is very easy to breed if only rice bran and dry yeast are available. If there is no problem in breeding prey, it does not cause big problem.

The reason for different kinds of mediums in raising nematodes and common thornbirds is to supply medium which is an optimal condition for maximizing the growth density by mixing various mediums in conventional breeding in case of feeding mites. The growth density of Hynobacterium japonicus matured in the existing medium is 20,000 ~ 25,000 grains / ml. However, it is the main purpose of using rice bran, which is inexpensive and easy to obtain from the surrounding area, rather than combining the optimal medium for self-production. And 15,000 ~ 20,000 grains / ml of the appropriate density that can not be obtained. It is also the same reason to use the rice husk rather than the expensive medium of peatmoss and vermiculite as the medium of the natural mite.

 The rice hulls are used as a medium for the bryophytes and the rice bran cultivation can be carried out only with rice bran and rice hull which can be easily obtained at the rice field. The cost incurred in breeding can also be a great advantage for the farm income, By producing this direct enemy and learning practical application technology and practicing it, the success rate of environmentally friendly cultivation is high, so it can promote the environment friendly agriculture, improve the quality of agricultural products through production and practical use of low cost natural enemies, and increase farmers' income.

The present invention also provides a composition for controlling insects, which comprises the above-mentioned method.

The present invention also provides a method for controlling pests of a crop using the composition.

In one embodiment of the present invention, the insect pests may be at least one selected from the group consisting of an insect pest, a root mussel, a root mussel, a small root fly and a slug, but is not limited thereto.

In one embodiment of the present invention, the crop is selected from the group consisting of cucumber, watermelon, lettuce, Poinsettia, onion, onion, paprika, strawberry, melon, melon, pumpkin, pepper, bell pepper, And perilla, but the present invention is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the present invention.

<Example 1> Self-production method of gibbous mites

<1-1> Feeding method of feeding mite (long mite mite)

For the self - production of Hypoaspis aculeifer, it was confirmed that Tyrophagus putrescentiae , a food mite of Thornbirds , was breeded on rice hull and dry yeast hobbles .

Specifically, the breeding box was divided into an outer box (FIG. 2) and a built-in box (FIG. 3). The dimensions of the breeding box are free, the outer box must have a cover, and when the outer box and the inner box are stacked, they should be spaced apart as shown in FIG. 4, and the height of the inner box is not enough to touch the cover of the outer box .

The medium to be used for the feeding of the broad-leaved mite mite, which is a feed mite, is mixed with rice bran (FIG. 5A) purchased from a general rice mill or a mill at a ratio of 100: 1 dry yeast (FIG. 5b. Dry yeast or baking yeast) at a ratio of 100: 1. Because it depends on the size of the built-in box, put a medium containing rice bran and dry yeast in 1/4 of the total volume of the box. Then, for one week, the proliferated Tyrophagus putrescentiae covers 1/4 of the total volume of the built-in box. It is better to keep about 1/2 of the total volume of the built-in box. Put the built-in box inside the external box, cover the external box cover, and leave it at about 20 ℃ ~ 30 ℃ (Optimum 25 ℃) for 1 week.

When one week passes, part of the mulberry mite is divided into branches for proliferation. Place a mixture of rice bran and dry yeast in a new box with 1/4 of the total volume of the box. It then covers a quarter of the total volume of the built-in box, which is grown for one week. The remaining plucked mite is fed by natural food.

Moisture treatment of the medium is a very difficult process in breeding since the water content of the medium is a very important factor in rearing in the breeding of the natural enemy company. In winter, the moisture content is increased because the external conditions are dry, and the moisture content is controlled by season, such as reducing the moisture content because the external conditions are humid in summer. However, the medium of the present invention does not need to be particularly treated with water. In order to maintain the moisture content of the medium, 0.5-1 cm of water is filled in the bottom of the outer box.

<1-2> Self-production method

The composition of the breeding box is an external box and a built-in box and proceeds in the same manner as the method of breeding the broad-leaved mite of Example 1-1. The breeding medium consists of rice hulls (Fig. 5c) and plum mullet, a feeding mite. Rice husks purchased from general mills or mills are sterilized by high pressure or subjected to high temperature drying or freezing treatment (more than one week at -20 ° C). Then add water to the rice hull at a ratio of 7: 3 or 6: 4 and mix well. Moisture is stored frozen in the freezer after treatment. Before use, thoroughly dissolve in room temperature before use.

One quarter of the total volume of the built-in box is covered with a chaff and three weeks of propagation. Thereafter, a feeder mite, broad-leaved mite, is supplied using a squeezer (Fig. 7A). It is better to keep about 1/2 of the total volume of the built-in box. Put the built-in box inside the external box, cover the external box cover, and leave it at about 20 ℃ ~ 30 ℃ (Optimum 25 ℃) for more than 3 weeks. In addition, food supply is provided in the same amount once a week. If you pass more than three weeks, you will be paid a portion. The branching method is the same as the above method. Feeding is done once a week to breed. When sufficient amount of ginseng for breeding is secured, ginseng thrips grown for 3 weeks or more are used for control.

The optimal prey rate for breeding of thistle mites is 1: 10 ~ 15 (nemesis: prey). In the case of conventional high density breeding, the feeding rate is two to three times a week, but in the case of low density breeding, feeding of food is only once a week. Generally, the growth density when cultivated on the optimum medium and in the cultivation condition is 20,000 ~ 25,000 grains / ml, but it is 15,000 ~ 20,000 grains / ml, which is lower than that of the grains. Assuming that the hatchlot density is 10 000 / ml (minimum 15,000 / ml) and the density of gibbous mite is 40 / ml (maximum 33.3 / ml), it is necessary to produce gibberish mites at home The percentage of specific amount of mite mite is summarized in Table 3 and Table 4 below.

division Minimum density Maximum density Chaff  1 box (ml) 3,000 3,000 3,000 3,000  ml / mari 10,000 15,000 20,000 25,000 Total number of grains per box 30,000,000 45,000,000 60,000,000 75,000,000 Thorn  1 box (ml) 4,000 4,000 4,000 4,000  ml / mari 25 30 35 40 Total number of grains per box 100,000 120,000 140,000 160,000

division Nemesis Feeding mite (long mite mite) Caretaker ratio One 10 11 12 13 14 15 Population (grains) 160,000 1,600,000 1,760,000 1,920,000 2,080,000 2,240,000 2,400,000 Per one enemy
Food supply (ml) 107 117 128 139 149 160 Available in a box of glutinous mite jelly 28.1 box 25.6 box 23.4 Box 21.6 Box 20.1 boxes 18.8 boxes

When the ratio of nematode: feed ratio is 1:15 when the minimum density is the maximum density and the maximum density of the mulberry mulberry, the mulberry mulberry is supplied at a rate of 160 ml per box of the mulberry jelly, and 18.8 boxes of the mulberry It can supply food for thorny mites. In addition, it can propagate more than twice (2.5 ~ 3 times) in a week because of a very strong growth mite.

<Example 2> Analysis of moisture content of the medium used in the self-production method of the mung bean mite

The water content of the medium used as the self-production method for the common mackerel mite of Example 1 was measured

The optimum moisture condition range of the medium used in the cultivation of thorny rhododendrons is between 25 and 40%. The moisture content of the medium was measured at 2 - day intervals using a moisture analyzer. In the conventional method, the medium was prepared so that the moisture content of the medium was more than 40% by using a moisture meter before the minute, and 300 ml of water was added to 700 ml of rice husk based on 1 L of the mulberry leaf midge medium. The water content of the culture medium was measured using a moisture analyzer for 4 weeks during the growth period at intervals of 2 days immediately after the culture.

As a result, it was confirmed that the water content in the double box form was maintained over 25% during the proliferation period of 4 weeks without the accurate measurement of the moisture content in the culture medium. It was confirmed that this was an easy method of producing the rearing medium and maintaining the moisture of the medium during the proliferation period (Fig. 8).

&Lt; Example 3 >

The enemy company produces the natural product at high density in order to raise the efficiency in a narrow space when producing the product. However, when using the natural enemies in the farmhouse, they are sold by reducing the density of the natural enemies by mixing the fillers again when packing the products after production to apply to the large area. In order to solve such problems as reducing unnecessary processes and decreasing the density due to the increase in the number of feeds (two or three times a week) and homo-predation (cannibalism) caused by high-density breeding, It is possible to use natural enemies that have been raised for 4 weeks in the farmhouse without any dilution of the filler. In addition, feeding of food can be provided only once a week. We investigated the density of gibbous mites in the conventional method and the double box method.

As a result, the difference in density between the high-density and low-density breeding methods occurs at the density of the mite mite (Fig. 9).

In addition, since the growth rate was over 300% at the 4th week of the proliferation, it was confirmed that the proliferation was possible at a density suitable for use as a natural product (Fig. 10).

Therefore, 10,000 bottles / 1,000 ml of the standard of the bottle type of mulberry hawthorn moths are available. This product can be used when the total moths per 10 ml of product are packed. At a low density of 33.3 cows / ml in the fourth week, the product is at the right density for immediate use at the farm.

While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments and that the scope of the present invention is not limited thereby. something to do. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (9)

1) breeding the breeding box by inserting the outer box and the built-in box by inserting the prey mite Tyrophagus putrescentiae and the breeding medium into the inner box; And
2) putting the hyacinth mites and rearing media in step 1) into a new built-in box, and then putting Hypoaspisaculeifer into the cover of the outer box to feed on the broad-leaved mite,
When the exterior box and the built-in box are stacked, the height of the built-in box does not touch the cover of the exterior box, and the exterior box has a cover to fill the exterior box and close the cover of the exterior box,
Wherein the ratio of the mulberry mulberry to the mulberry mulberry is from 1:10 to 1:15. delete The method according to claim 1,
The method according to any one of claims 1 to 3, wherein the cultivation medium of the step 1) comprises rice bran, pressed wheat or cereal flour, cereal by-products and dry yeast The method according to claim 1,
The method according to any one of claims 1 to 3, wherein the step (2) comprises feeding the rice husk or filler, sawdust, and the mildew mite raised in step (1). delete delete delete delete delete

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