CN103782969B - A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed - Google Patents
A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed Download PDFInfo
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- CN103782969B CN103782969B CN201410074195.4A CN201410074195A CN103782969B CN 103782969 B CN103782969 B CN 103782969B CN 201410074195 A CN201410074195 A CN 201410074195A CN 103782969 B CN103782969 B CN 103782969B
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- 241001248473 Trichogramma pintoi Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000012258 culturing Methods 0.000 title abstract description 10
- 235000013312 flour Nutrition 0.000 claims abstract description 69
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 48
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 48
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- 235000005822 corn Nutrition 0.000 claims abstract description 32
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- 235000013601 eggs Nutrition 0.000 claims description 77
- 238000009395 breeding Methods 0.000 claims description 49
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- 241000256618 Trichogramma Species 0.000 claims description 15
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- 241000326498 Ophiorrhabda cellifera Species 0.000 claims description 10
- 240000002044 Rhizophora apiculata Species 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 238000012546 transfer Methods 0.000 claims description 10
- 241000607479 Yersinia pestis Species 0.000 claims description 7
- 229920000742 Cotton Polymers 0.000 claims description 5
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Abstract
The invention provides a kind of method and rice moth culturing feed of indoor propagation Trichogramma pintoi, rice moth culturing feed divides A feed and B feed, the raw material composition of A feed and parts by weight: brown shorts 50 parts, corn flour 40 parts, 10 parts, flour and 5 parts, water; The raw material composition of B feed and parts by weight: brown shorts 50 parts, corn flour 40 parts, 5 parts, flour, fish meal 3 parts, dusty yeast 2 parts and 5 parts, water.Propagation method take rice moth egg as vector, adopt the formula forage of multiple feed formulation rice moth different times larva, raising rice moth larva by formula forage makes adult eclosion neat, and egg laying amount is high, adopts the mode of Boiling tube access ovum card to carry out a large amount of inoculation reproduction oophagous trichogrammae.The present invention does not need large-scale instrument, and propagating materials used is simple and easy to get, simple to operate, practical, environmentally friendly, pollution-free, and the Trichogramma pintoi produced can be used for the biological control of multiple agriculture and forestry injurious insect, has good economy, ecology and social benefit.
Description
Technical field
The invention belongs to agriculture and forestry injurious insect technical field of biological control, relate to a kind of method and rice moth culturing feed of indoor propagation Trichogramma pintoi.
Background technology
Oophagous trichogrammae (
trichogrammaspp.) discovery, can the ovum of parasitic multiple lepidopterous insects apart from modern existing more than 100 year, is a class ovum parasite most widely used in whole world biological control of insect pests.China has just carried out application study to oophagous trichogrammae from nineteen thirties, from 1934, Zhu Ruzuo, Hu Yongxi have found that oophagous trichogrammae was stopped to the seventies at Zhejiang Province Tang Xixian in pine tag worm's ovum, and the Pu dragon etc. that stings has carried out systematic research to the host of oophagous trichogrammae, large scale artificial breeding and field bulk storage effect etc.After the eighties, China's oophagous trichogrammae investigation and application technology obtains further reinforcement, and the application aspect of oophagous trichogrammae classification, behavior, physiological ecological and artificial host egg has carried out a large amount of work.Along with improving constantly of the development of sustainable agriculture and the requirement of pollution-free green food industry, this biological prevention based on natural enemy more and more accept by vast farmers.Trichogramma pintoi is the natural enemy that a class has value very much, can be used for the multiple agriculture and forestry injurious insect biological controls such as cotton bollworm, beet webworm, cutworm, steinernema.The institute of agricultural sciences of Yuncheng Prefecture of Shanxi Province to Trichogramma pintoi biological characteristics seek peace parasitic cotton bollworm condition survey observe; Wang Fucheng, a comb instrument produces the research of the Trichogramma pintoi of both sexes filial generation to parthenogenesis; Ma Deying etc. to Trichogramma pintoi control Cotton Boll Worm Populations From Xinjiang Uygur Autonomous Region Preliminary Report on Experiment, and have carried out the influence research of term refrigeration to different lines Trichogramma pintoi; Tian Xiaoxia etc. are at the parasitic beet webworm ovum of China's Late Cambrian Trichogramma pintoi; Shen Jian, permitted for army building etc. to have carried out the impact that ultraviolet process oriental fruit moth ovum is parasitic on Trichogramma pintoi and sprout wings, high temperature stress is studied the parasitization of oriental fruit moth ovum the impact of Trichogramma pintoi parasitic functional response and Trichogramma pintoi.And for Trichogramma pintoi artificial propagation techniques except coroner is all auspicious etc. describe a kind of utilize the method for oriental fruit moth ovum indoor propagation Trichogramma pintoi except do not see that other is reported, and the method is difficult to large-scale production and is applied to biological control of insect pests.
Summary of the invention
The object of this invention is to provide and a kind ofly utilize rice moth egg to carry out the method for amount reproduction Trichogramma pintoi in indoor efficiently; Another object of the present invention is to provide a kind of rice moth feed efficiently.
In order to achieve the above object, the present invention adopts following technical scheme:
A rice moth culturing feed for indoor propagation Trichogramma pintoi, rice moth culturing feed divides A feed and B feed, and its preparation raw material composition and parts by weight are respectively:
The raw material composition of A feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 10 parts, flour and 5 parts, water;
The raw material composition of B feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 5 parts, flour, fish meal 3 parts, dusty yeast 2 parts and 5 parts, water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heated, temperature is sterilized at keeping 60-90 DEG C 4-5 hour, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour and flour and fully mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heated, temperature is sterilized at keeping 60-90 DEG C 4-5 hour, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour, flour, fish meal and fully mix thoroughly again together with dusty yeast and obtain B feed.
A kind of method of indoor propagation Trichogramma pintoi, in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, indoor collection Trichogramma pintoi is returned as honeybee kind provenance using by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg in indoor, propagation method comprises the following steps:
(1) breeding of rice moth: during rice moth inoculation, at the A feed that plastic crate middle berth one deck 1.1-1.2 cm is thick, feed is evenly spilt rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, and be placed in rice moth and raise indoor feeding, the larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, A feed is thrown in inoculation, throw in B feed afterwards, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa is collected adult after sprouting wings and is placed in cage of laying eggs and lays eggs, and every day collects rice moth egg, and part is directly used in the breeding of rice moth, and remainder ultraviolet light performs the refrigerator that mark puts into 3-5 DEG C and preserves after killing embryo, be made into rice moth egg card before numerous honeybee, for subsequent use;
(2) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle collections Trichogramma pintoi as honeybee kind provenance, in the bottle collecting honeybee kind, access rice moth egg card, parasitism breeds into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(3) breeding of oophagous trichogrammae: 1 oophagous trichogrammae breeding kind of honeybee card is put into breeding bee tube in numerous honeybee indoor, the mouth of pipe is clogged with cotton plug, when the bee eclosion of honeybee card upper 60-70 %, access 8 rice moth eggs at transfer room snap in breeding bee tube and allow Trichogramma, put into the filter paper bar sweeping 10 % hydromels to feed simultaneously, during access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes having accessed ovum card are palletized and covers shading with black cloth, then move into numerous honeybee indoor to lay eggs, in transfer room, 8 new rice moth egg cards are changed to same inoculation method after 8-14 hour, often open breeding kind of a honeybee card and can connect 16 rice moth egg cards, postvaccinal rice moth egg card stands to be put in capsule and to move into numerous honeybee indoor and continues to breed 4-5 days, then the refrigerator putting into 3-5 DEG C carries out short-term preservation, the indoor cultivation of numerous honeybee is placed in shifting to an earlier date 4-7 days during honeybee from refrigerator and taking out, it is numerous or for biological control of insect pests that honeybee after cultivation blocks available expansion.
It is 25-28 DEG C that described rice moth receptacle controls indoor temperature, and relative moisture is 60-85 %.
It is 25-28 DEG C that described numerous honeybee room controls indoor temperature, and relative moisture is 70-85 %.
Described breeding bee tube is the Boiling tube of diameter 30 mm × length 20 cm.
Described rice moth egg card is that the strip label card of 2 cm × 4 cm glues rice moth egg and makes.
Advantage of the present invention and beneficial effect have:
(1) Trichogramma pintoi that the present invention breeds is the extensive natural enemy insect of a class host, can be used in the biological control of multiple agriculture and forestry injurious insect.
(2) with the addition of fish meal in larvae feeds in rice moth of the present invention breeding, that dusty yeast makes rice moth imago sprout wings is more neat, egg laying amount is higher.
(3) make the parasitic rate of oophagous trichogrammae higher with the numerous honeybee of Boiling tube, mean parasitized rate reaches more than 90%.
(4) the present invention is without large-scale instrument, and propagating materials used easily obtains, simple to operate, practical.
(5) the present invention is environmentally friendly, pollution-free, is suitable for application and promotes, having good social benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described.
embodiment 1:
One, the rice moth culturing feed of Trichogramma pintoi is bred:
The raw material composition of A feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 10 parts, flour and 5 parts, water;
The raw material composition of B feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 5 parts, flour, fish meal 3 parts, dusty yeast 2 parts and 5 parts, water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heated, sterilize 5 hours at temperature keeps 60 DEG C, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour and flour and fully mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heated, sterilize 5 hours at temperature keeps 60 DEG C, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour, flour, fish meal and fully mix thoroughly again together with dusty yeast and obtain B feed.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, return indoor collection Trichogramma pintoi as honeybee kind provenance using by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg in indoor, propagation method comprises the following steps:
(1) breeding of rice moth: during rice moth inoculation, at the A feed that plastic crate middle berth one deck 1.1cm is thick, feed is evenly spilt rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 25 DEG C, relative moisture is 70 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, A feed is thrown in inoculation, throw in B feed afterwards, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa emergence rear collection adult is placed in cage of laying eggs and lays eggs, every day collects rice moth egg, part is directly used in the breeding of rice moth, remainder ultraviolet light performs the refrigerator that mark puts into 3 DEG C and preserves after killing embryo, glue rice moth egg with the strip label card of 2 cm × 4 cm before numerous honeybee and be made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle collections Trichogramma pintoi as honeybee kind provenance, in the bottle collecting honeybee kind, access rice moth egg card, parasitism breeds into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, the mouth of pipe is clogged with cotton plug, when the bee eclosion of honeybee card upper 60%, access 8 rice moth eggs at transfer room snap in breeding bee tube and allow Trichogramma, put into the filter paper bar sweeping 10 % hydromels to feed simultaneously, during access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes having accessed ovum card are palletized and covers shading with black cloth, then move into numerous honeybee indoor to lay eggs, controlling indoor temperature is 25 DEG C, relative moisture is 70 %, in transfer room, 8 new rice moth egg cards are changed to same inoculation method after 8 hours, often open breeding kind of a honeybee card and can connect 16 rice moth egg cards, postvaccinal rice moth egg card stands to be put in capsule and to move into numerous honeybee indoor and continues to breed 4 days, mean parasitized rate reaches 90.6%, then the refrigerator parasitic rice moth egg card being put into 3 DEG C carries out short-term preservation, the indoor cultivation of numerous honeybee is placed in shifting to an earlier date 7 days during honeybee from refrigerator and taking out, it is numerous or for biological control of insect pests that honeybee after cultivation blocks available expansion.
embodiment 2:
One, the rice moth culturing feed of Trichogramma pintoi is bred:
The raw material composition of A feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 10 parts, flour and 5 parts, water;
The raw material composition of B feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 5 parts, flour, fish meal 3 parts, dusty yeast 2 parts and 5 parts, water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heated, sterilize 5 hours at temperature keeps 70 DEG C, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour and flour and fully mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heated, sterilize 5 hours at temperature keeps 70 DEG C, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour, flour, fish meal and fully mix thoroughly again together with dusty yeast and obtain B feed.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, return indoor collection Trichogramma pintoi as honeybee kind provenance using by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg in indoor, propagation method comprises the following steps:
(1) breeding of rice moth: during rice moth inoculation, at the A feed that plastic crate middle berth one deck 1.1cm is thick, feed is evenly spilt rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 26 DEG C, relative moisture is 75 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, A feed is thrown in inoculation, throw in B feed afterwards, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa emergence rear collection adult is placed in cage of laying eggs and lays eggs, every day collects rice moth egg, part is directly used in the breeding of rice moth, remainder ultraviolet light performs the refrigerator that mark puts into 4 DEG C and preserves after killing embryo, glue rice moth egg with the strip label card of 2 cm × 4 cm before numerous honeybee and be made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle collections Trichogramma pintoi as honeybee kind provenance, in the bottle collecting honeybee kind, access rice moth egg card, parasitism breeds into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, the mouth of pipe is clogged with cotton plug, when the bee eclosion of honeybee card upper 65 %, access 8 rice moth eggs at transfer room snap in breeding bee tube and allow Trichogramma, put into the filter paper bar sweeping 10 % hydromels to feed simultaneously, during access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes having accessed ovum card are palletized and covers shading with black cloth, then move into numerous honeybee indoor to lay eggs, controlling indoor temperature is 26 DEG C, relative moisture is 75 %, in transfer room, 8 new rice moth egg cards are changed to same inoculation method after 10 hours, often open breeding kind of a honeybee card and can connect 16 rice moth egg cards, postvaccinal rice moth egg card stands to be put in capsule and to move into numerous honeybee indoor and continues to breed 5 days, mean parasitized rate reaches 92.1%, then the refrigerator parasitic rice moth egg card being put into 4 DEG C carries out short-term preservation, the indoor cultivation of numerous honeybee is placed in shifting to an earlier date 5 days during honeybee from refrigerator and taking out, it is numerous or for biological control of insect pests that honeybee after cultivation blocks available expansion.
embodiment 3:
One, the rice moth culturing feed of Trichogramma pintoi is bred:
The raw material composition of A feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 10 parts, flour and 5 parts, water;
The raw material composition of B feed and parts by weight:
Brown shorts 50 parts, corn flour 40 parts, 5 parts, flour, fish meal 3 parts, dusty yeast 2 parts and 5 parts, water.
Described A feed and the preparation method of B feed be respectively:
(1) preparation of A feed: first the brown shorts of A feedstuff composition, corn flour, flour are put into disinfection cabinet and heated, sterilize 4 hours at temperature keeps 90 DEG C, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour and flour and fully mix thoroughly again and obtain A feed;
(2) preparation of B feed: first the brown shorts of B feedstuff composition, corn flour, flour, fish meal are put into disinfection cabinet and heated, sterilize 4 hours at temperature keeps 90 DEG C, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts and water are fully mixed thoroughly, then add corn flour, flour, fish meal and fully mix thoroughly again together with dusty yeast and obtain B feed.
Two, the method for indoor propagation Trichogramma pintoi:
In mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, return indoor collection Trichogramma pintoi as honeybee kind provenance using by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg in indoor, propagation method comprises the following steps:
(1) breeding of rice moth: during rice moth inoculation, at the A feed that plastic crate middle berth one deck 1.2 cm is thick, feed is evenly spilt rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, be placed in rice moth and raise indoor feeding, controlling indoor temperature is 28 DEG C, relative moisture is 80 %, larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, A feed is thrown in inoculation, throw in B feed afterwards, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa emergence rear collection adult is placed in cage of laying eggs and lays eggs, every day collects rice moth egg, part is directly used in the breeding of rice moth, remainder ultraviolet light performs the refrigerator that mark puts into 5 DEG C and preserves after killing embryo, glue rice moth egg with the strip label card of 2 cm × 4 cm before numerous honeybee and be made into rice moth egg card, for subsequent use;
(3) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle collections Trichogramma pintoi as honeybee kind provenance, in the bottle collecting honeybee kind, access rice moth egg card, parasitism breeds into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(4) breeding of oophagous trichogrammae: at the indoor breeding bee tube 1 oophagous trichogrammae breeding kind of honeybee card being put into diameter 30mm × long 20cm of numerous honeybee, the mouth of pipe is clogged with cotton plug, when the bee eclosion of honeybee card upper 70%, access 8 rice moth eggs at transfer room snap in breeding bee tube and allow Trichogramma, put into the filter paper bar sweeping 10 % hydromels to feed simultaneously, during access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes having accessed ovum card are palletized and covers shading with black cloth, then move into numerous honeybee indoor to lay eggs, controlling indoor temperature is 28 DEG C, relative moisture is 80 %, in transfer room, 8 new rice moth egg cards are changed to same inoculation method after 12 hours, often open breeding kind of a honeybee card and can connect 16 rice moth egg cards, postvaccinal rice moth egg card stands to be put in capsule and to move into numerous honeybee indoor and continues to breed 4 days, mean parasitized rate reaches 91.3%, then the refrigerator parasitic rice moth egg card being put into 5 DEG C carries out short-term preservation, the indoor cultivation of numerous honeybee is placed in shifting to an earlier date 6 days during honeybee from refrigerator and taking out, it is numerous or for biological control of insect pests that honeybee after cultivation blocks available expansion.
Claims (7)
1. the method for an indoor propagation Trichogramma pintoi, it is characterized in that: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, indoor collection Trichogramma pintoi is returned as honeybee kind provenance using by the ovum collection of Trichogramma, breed in a large number as vector using rice moth egg in indoor, propagation method comprises the following steps:
(1) breeding of rice moth: during rice moth inoculation, at the A feed that plastic crate middle berth one deck 1.1-1.2cm is thick, feed is evenly spilt rice moth kind ovum, free-and-easy one deck just covers the flour bisque of ovum grain again, and be placed in rice moth and raise indoor feeding, the larva after hatching starts feeding after eating out thickly dotted aperture, within first 15 days, A feed is thrown in inoculation, throw in B feed afterwards, within every 3 days, throw a defective material, input amount is that the larva appetite of 3 days is until larvae pupation; Pupa is collected adult after sprouting wings and is placed in cage of laying eggs and lays eggs, and every day collects rice moth egg, and part is directly used in the breeding of rice moth, and remainder ultraviolet light performs the refrigerator that mark puts into 3-5 DEG C and preserves after killing embryo, be made into rice moth egg card before numerous honeybee, for subsequent use;
(2) field bee kind: in mangrove forest Lasiognatha cellifera Adult worms producting eggs peak period, gather by the ovum of Trichogramma, return indoor bottle collections Trichogramma pintoi as honeybee kind provenance, in the bottle collecting honeybee kind, access rice moth egg card, parasitism breeds into oophagous trichogrammae breeding kind of honeybee card, for subsequent use;
(3) breeding of oophagous trichogrammae: 1 oophagous trichogrammae breeding kind of honeybee card is put into breeding bee tube in numerous honeybee indoor, the mouth of pipe is clogged with cotton plug, when the bee eclosion of honeybee card upper 60-70%, access 8 rice moth eggs at transfer room snap in breeding bee tube and allow Trichogramma, put into the filter paper bar sweeping 10% hydromel to feed simultaneously, during access ovum card, the other end of the mouth of pipe is to light, and anti-kind of honeybee escapes from, after having inoculated, all breeding bee tubes having accessed ovum card are palletized and covers shading with black cloth, then move into numerous honeybee indoor to lay eggs, in transfer room, 8 new rice moth egg cards are changed to same inoculation method after 8-14 hour, often open breeding kind of a honeybee card and can connect 16 rice moth egg cards, postvaccinal rice moth egg card stands to be put in capsule and to move into numerous honeybee indoor and continues to breed 4-5 days, then the refrigerator putting into 3-5 DEG C carries out short-term preservation, the indoor cultivation of numerous honeybee is placed in shifting to an earlier date 4-7 days during honeybee from refrigerator and taking out, it is numerous or for biological control of insect pests that honeybee after cultivation blocks available expansion,
The raw material components of described A feed and parts by weight: brown shorts 50 parts, corn flour 40 parts, 10 parts, flour and 5 parts, water;
The raw material components of described B feed and parts by weight: brown shorts 50 parts, corn flour 40 parts, 5 parts, flour, fish meal 3 parts, dusty yeast 2 parts and 5 parts, water.
2. the method for a kind of indoor propagation Trichogramma pintoi according to claim 1, is characterized in that: it is 25-28 DEG C that described rice moth receptacle controls indoor temperature, and relative moisture is 60-85%.
3. the method for a kind of indoor propagation Trichogramma pintoi according to claim 1, is characterized in that: it is 25-28 DEG C that described numerous honeybee room controls indoor temperature, and relative moisture is 70-85%.
4. the method for a kind of indoor propagation Trichogramma pintoi according to claim 1, is characterized in that: described breeding bee tube is the Boiling tube of diameter 30mm × long 20cm.
5. the method for a kind of indoor propagation Trichogramma pintoi according to claim 1, is characterized in that: described rice moth egg card is that the strip label card of 2cm × 4cm glues rice moth egg and makes.
6. the method for a kind of indoor propagation Trichogramma pintoi according to claim 1, it is characterized in that: the preparation of described A feed is: first the brown shorts 50 parts of A feedstuff component, corn flour 40 parts, 10 parts, flour are put into disinfection cabinet respectively and heated, temperature is sterilized at keeping 60-90 DEG C 4-5 hour, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts 50 parts and 5 parts, water are fully mixed thoroughly, then add corn flour 40 parts and 10 parts, flour and fully mix thoroughly again and obtain A feed.
7. the method for a kind of indoor propagation Trichogramma pintoi according to claim 1, it is characterized in that: the preparation of described B feed: first the brown shorts 50 parts of B feedstuff component, corn flour 40 parts, 5 parts, flour are put into disinfection cabinet and heated, temperature is sterilized at keeping 60-90 DEG C 4-5 hour, kill the germ in feed, insect, evil mite and ovum thereof, again brown shorts 50 parts and 5 parts, water are fully mixed thoroughly, then add corn flour 40 parts, 5 parts, flour, fish meal 3 parts and fully mix thoroughly again together with dusty yeast 2 parts and obtain B feed.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612945A (en) * | 2019-10-19 | 2019-12-27 | 山西大学 | Device and method for stabilizing quantity of trichogramma of corn borer in field |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123598A (en) * | 1994-11-28 | 1996-06-05 | 吉林农业大学 | Key technique for large scale production of parasitic bees |
| CN1285142A (en) * | 1999-08-19 | 2001-02-28 | 中国农业科学院生物防治研究所 | Technology for mass rearing grained moth |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
| EP2248417A2 (en) * | 2009-05-08 | 2010-11-10 | Agrobio S.L. | Process for rearing a species of indigenous parasitoid and use thereof for controlling lepidopteran crop pest |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
| DE102010044601A1 (en) * | 2010-09-07 | 2012-03-08 | BIOCARE Gesellschaft für biologische Schutzmittel mbH | Method for the biological control of pest organisms in a agricultural, horticultural or forestry plant stock |
| CN103283540A (en) * | 2013-06-26 | 2013-09-11 | 广西壮族自治区林业科学研究院 | Method for biological control over mangrove plants primary pest Aegiceras Chaetognatha tortricidae small leaf rollers |
-
2014
- 2014-03-03 CN CN201410074195.4A patent/CN103782969B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1123598A (en) * | 1994-11-28 | 1996-06-05 | 吉林农业大学 | Key technique for large scale production of parasitic bees |
| CN1285142A (en) * | 1999-08-19 | 2001-02-28 | 中国农业科学院生物防治研究所 | Technology for mass rearing grained moth |
| CN101438691A (en) * | 2007-11-23 | 2009-05-27 | 北京市农林科学院 | Mass propagation method of Oophagous trichogrammae from artificial ovum |
| EP2248417A2 (en) * | 2009-05-08 | 2010-11-10 | Agrobio S.L. | Process for rearing a species of indigenous parasitoid and use thereof for controlling lepidopteran crop pest |
| DE102010044601A1 (en) * | 2010-09-07 | 2012-03-08 | BIOCARE Gesellschaft für biologische Schutzmittel mbH | Method for the biological control of pest organisms in a agricultural, horticultural or forestry plant stock |
| CN102106319A (en) * | 2011-01-18 | 2011-06-29 | 西北农林科技大学 | Method for propagating trichogramma pintoi voegele indoors by grapholitha molesta busck egg |
| CN103283540A (en) * | 2013-06-26 | 2013-09-11 | 广西壮族自治区林业科学研究院 | Method for biological control over mangrove plants primary pest Aegiceras Chaetognatha tortricidae small leaf rollers |
Non-Patent Citations (3)
| Title |
|---|
| 不同饲料配方对米蛾生长发育及繁殖的影响;张俊杰等;《吉林农业大学学报》;20121107;第34卷(第6期);第603-606、611页 * |
| 暗黑赤眼蜂对梨小食心虫卵的寄生作用_;沈健等;《植物保护学报》;20120815;第39卷(第4期);第352-356页 * |
| 米蛾饲料配方筛选研究;杨长成等;《沈阳农业大学学报》;19900402;第21卷(第1期);第48-52页 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612945A (en) * | 2019-10-19 | 2019-12-27 | 山西大学 | Device and method for stabilizing quantity of trichogramma of corn borer in field |
| CN110612945B (en) * | 2019-10-19 | 2020-07-10 | 山西大学 | Device and method for stabilizing quantity of trichogramma of corn borer in field |
| CN111011313A (en) * | 2019-12-30 | 2020-04-17 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
| CN111011313B (en) * | 2019-12-30 | 2022-04-19 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
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