CN105145487B - The artificial breeding production method of Trichogramma - Google Patents
The artificial breeding production method of Trichogramma Download PDFInfo
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- CN105145487B CN105145487B CN201410354251.XA CN201410354251A CN105145487B CN 105145487 B CN105145487 B CN 105145487B CN 201410354251 A CN201410354251 A CN 201410354251A CN 105145487 B CN105145487 B CN 105145487B
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Abstract
The invention provides a kind of method that Trichogramma is bred using Beet Armyworm Eggs, method production efficiency provided by the invention is higher, and the breeding cycle is short, is advantageous to large-scale production Trichogramma.It the described method comprises the following steps：1) Beet Armyworm Eggs are produced；2) Trichogramma is bred using Beet Armyworm Eggs.For methods described using the Trichogramma preventing and treating agricultural pests of artificial breeding, the agricultural pests are mainly rice-stem borer, yellow rice borer, rice leaf roller.Compared with prior art, the present invention has advantages below：Beet armyworm source is easy, raising is easy；Method production efficiency provided by the invention is higher, and the breeding cycle is short, is easy to breed on a large scale, is advantageous to large-scale production Trichogramma.
Description
Technical field
The invention belongs to the technical field of biological control of agricultural pests, is related to the breeding production method of trichogramma, especially relates to
A kind of and artificial breeding production method of Trichogramma.
Background technology
Trichogramma is most study in whole world biological control of insect pests, most widely used a kind of insect, at present at least 12
Kind by large scale artificial breeding, to prevent and treat some important agriculture and forestry injurious insects.The a large amount of of trichogramma population are bred in mainly utilization
Between host egg carry out, the vector ovum of trichogramma is using more successfully having egg of oak silkworm, Eggs of Philosamia Cynthia Ricini, gelechiid ova and rice moth
Ovum.Big ovum (egg of oak silkworm, Eggs of Philosamia Cynthia Ricini) is easy due to obtaining, advantage of lower cost, so the big ovum bred using egg of oak silkworm etc.
Honeybee such as trichogramma dendrolimi energy large-scale application.Using the trichogramma dendrolimi preventing and treating insect such as corn borer be China with most into
The biological and ecological methods to prevent plant disease, pests, and erosion technology of work(, the gross area is prevented and treated more than 45,000,000 mu in China northeast, North China at present, and production technology is ripe, factory
(station) more than 10 family, input-output ratio are up to more than 1: 20, and " public plant protection, green are tentatively realized in corn borer preventing and controlling
The target of plant protection ".
Trichogramma is the important parasite of the rice grubs such as rice-stem borer, yellow rice borer and rice leaf roller,
The host range of Trichogramma is narrow, is still had any problem with striped rice borer and rice leaf roller these primary host's mass rearings,
Typically Trichogramma is bred by the use of rice moth egg as Hosts, but there is also large-scale production to be stranded for the production model
The defects of difficult, host breeding cycle is longer, China for many years, but are hardly formed rule using rice moth egg production Trichogramma
Modelling, commercialization, limit the production of organic paddy rice.The parasitic egg stage of Ostrinia furnacalis of Trichogramma energy and mythimna separata ovum, but both
The ovum of insect is not easy to remove from spawning utensil ovum card is made into bulk, limits application；Diamondback moth life cycle is short, breeding
Power is high, can expand population rapidly in a short time, obtain a large amount of ovum, but its ovum grain is small, causes the Trichogramma quality bred
Decline, equally, Eggs of Diamondback Moth is not easy to that ovum card is made, and forms standardization；The egg laying amount of prodenia litura is big, and mark also can easily be made
Accurate ovum card, but can not complete to develop after Trichogramma parasitism.Therefore screening a kind of high Hosts of production efficiency is
An important foundation of rice terrace biological control is used as by the use of trichogramma.
The content of the invention
Based on object above, the invention provides a kind of artificial breeding production method of Trichogramma.The present invention provides
A kind of method that Trichogramma is bred using Beet Armyworm Eggs, method production efficiency provided by the invention is higher, breeding week
Phase is short, is advantageous to large-scale production Trichogramma.
On the one hand, the invention provides a kind of artificial breeding production method of Trichogramma, wherein, methods described includes
The step of Trichogramma being bred using Beet Armyworm Eggs.
Preferably, the described method comprises the following steps：
1) Beet Armyworm Eggs are produced；
2) Trichogramma is bred using Beet Armyworm Eggs.
Preferably, the step 1) includes the raising of beet exigua larvae, the collection of pupa, imago breeding, the collection of ovum；
Preferably, the temperature that the condition of raising is 24-30 DEG C in the step 1), 60%-70% humidity；More preferably
Ground, temperature are 27 ± 1 DEG C, humidity 65%；
It is highly preferred that the step 1) comprises the following steps：
A) Beet Armyworm Eggs that selection will hatch, which are put into hatching house, hatches, and hatches 2-3 days；
Preferably, feed is added in the larvae hatch room；
Preferably, the temperature of the hatching house is 24-30 DEG C, humidity 60%-70%；It is highly preferred that temperature is 27 ± 1
DEG C, humidity 65%；
B) after hatching, regrowth 4-6 days, larva is transferred to the raising of larva raising room, and adds feed, the larva raising phase
For 10-15 days；
Preferably, the feed is the man-made feeds suitable for lepidopteran insect larvae, in a preferred embodiment,
The material and method system that feed used in this application provides in the Chinese patent application according to Patent No. 200410039466.9
It is standby；
C) pupate after completing at least one day, by the pupa of beet armyworm, sterilize 20-40 minutes using 4% formalin, dry in the air
It is dry；Wherein beet armyworm pupa time is 5-7 days；
D) before pupa emergence, pupa is put into laying coop, wherein, 1-3 days early stage of Adult worms producting eggs, 4-6 days egg-laying season.
Preferably, the temperature of the laying coop is 24-30 DEG C, humidity 60%-70%；It is highly preferred that temperature is 27 ± 1
DEG C, humidity 65%；
E) Beet Armyworm Eggs are collected.
Preferably, Beet Armyworm Eggs described in selected part are used for conservation；
Preferably, 20-40 minutes will be sterilized with 4% formalin for the Beet Armyworm Eggs of conservation, after drying, for standby
Raise beet armyworm.
It is highly preferred that simple grain will be divided into for the Beet Armyworm Eggs for connecing honeybee, ovum card is made.
Preferably, the every square centimeter of the ovum card has 380 ± 20 ovum of Beet Armyworm Eggs；
Preferably, the Beet Armyworm Eggs are divided into simple grain using 40 mesh sieves；
Preferably, the ovum card under ultraviolet using inactivating；
It is highly preferred that the inactivation time is 20-40 minutes；
Preferably, the step 2) is：
I) in numerous honeybee room, 24-30 DEG C of keeping temperature, humidity 70%-90%, according to the kind honeybee of Trichogramma and beet
Noctuid ovum is than 1:5~15 breed, and are taken out after 1 day；
Preferably, numerous honeybee room temperature is maintained at 27 ± 1 DEG C, humidity about 80%；
Preferably, numerous honeybee room keeps dark state.
II) egg parasitoid for completing spawning process is put into culturing room, before cultivating to Trichogramma development to pupa, then
Storage.
Preferably, the temperature of the culturing room is maintained at 24-30 DEG C, humidity 70%-90%；It is highly preferred that the culture
Room temperature is maintained at 27 ± 1 DEG C, humidity about 80%；
Preferably, by it is described save as being placed in 5-7 DEG C of refrigerating box store.
Preferably, bred in the step 2) using Beet Armyworm Eggs card, then store ovum card；
Preferably, the Trichogramma egg parasitoid that spawning process is completed in step 2) is prepared as ovum card；
Preferably, the every square centimeter of the ovum card has Trichogramma egg parasitoid 380 ± 20；
Preferably, the ovum card under ultraviolet using inactivating；It is highly preferred that the inactivation time is 20-40 minutes；
Preferably, before the ovum is stuck in field adaptability, in 27 ± 1 DEG C of temperature, humidity 70%-90% heating developments；
Wherein, the condition such as pest development progress, local weather forecast that the time of the heating is prevented and treated according to each field
Determined after considering.
On the other hand, present invention also offers a kind of method for preventing agricultural pests, wherein methods described to use artificial breeding
The Trichogramma preventing and treating agricultural pests educated, the agricultural pests are mainly rice-stem borer, yellow rice borer, rice leaf roller.
Compared with prior art, the present invention has advantages below：
1) the beet armyworm source that is used in the inventive method easily, raising it is easy；
2) method production efficiency provided by the invention is higher, and the breeding cycle is short, is easy to breed on a large scale, is advantageous to scale
Produce Trichogramma.
Brief description of the drawings
Hereinafter, embodiment of the present invention is described in detail with reference to accompanying drawing, wherein：
Fig. 1 is the flow chart of the specific embodiment of the present invention；
Embodiment
Unless specifically stated otherwise, Beet Armyworm Eggs used in following examples are purchased from animal institute of the Chinese Academy of Sciences.
Unless specifically stated otherwise, composition used in following examples is the composition for analyzing pure rank, and can be from just
Often commercially available channel obtains.
Embodiment 1 produces Beet Armyworm Eggs
1. the raising of beet armyworm, insectary temperature control is at 27 ± 1 DEG C, humidity 65% or so.
The raising of A larvas；The Beet Armyworm Eggs soon hatched are placed on culture dish side, and added in right amount in opposite side
Man-made feeds, the lid with toilet paper is covered, in case larva escapes.Per about 0.1 gram of ovum of ware, after hatching 2 days, by the children of a ware
Worm can split in 2 or 3 wares and raise；After regrowth 2-3 days, larva is transferred in big square box and raised, 2-3 wares larva can merge
Into one big square box, and add man-made feeds；Each big square box larva quantity can be adjusted according to actual conditions, per big before pupating
Square box there are about 400 or so larvas, about 12 days beet exigua larvae phases.Before pupating, by multilayer toilet paper place mat in big square box one
Side, it is set smoothly to pupate.
B receives pupa；Pupate after completing about 1 day, tear toilet paper, shake off the pupa of beet armyworm, the larva not pupated is abandoned,
Debris is picked up clearly, pupa is sterilized 30 minutes with 4% formalin, dries.About 7 days beet armyworm pupa time.
C Adult worms producting eggs；Before emergence, pupa is put into spawning cage, after emergence, feeds 10% hydromel, is put into spawning modeling
Pocket, beet armyworm lay eggs on polybag, about 2 days early stages of Adult worms producting eggs, about 5 days or so egg-laying season
The collection processing of D ovum；Polybag is overturn, shakes off and shakes loose the Beet Armyworm Eggs on polybag.A part of ovum can
To be sterilized 30 minutes with 4% formalin, after drying, for breeding beet armyworm.
Embodiment 2 breeds Trichogramma
The making of 1 ovum card, 40 mesh sieves will be crossed for connecing the Beet Armyworm Eggs of honeybee, be divided into simple grain, ovum is poured on and scribbles glue
Or with being stained with the scraps of paper of double faced adhesive tape, ovum card is made, dries, (every square centimeter to there are about 380 ovum of Beet Armyworm Eggs), 15
Inactivated 30 minutes under watt ultraviolet.
2. numerous honeybee room temperature is maintained at 27 ± 1 DEG C, humidity about 80%, because Trichogramma has phototaxis, typically exist
It is dark to connect numerous honeybee room during honeybee.By the ovum card made according to honeybee ovum than 1:10 are put into and connect in honeybee box, are taken out after 1 day.It is put into daily
Kind honeybee and new ovum card.The ovum for having connect honeybee is stuck at 27 ± 1 DEG C and placed 3 days, treats that Trichogramma is developed to before pupa.It is stored in 5-
In 7 DEG C of refrigerating box.
Embodiment 3 is compared with the prior art and cost accounting
The parasitic egg stage of Ostrinia furnacalis of Trichogramma energy and mythimna separata ovum, but the ovum of both insects is not easy from spawning into bulk
Removed on utensil and ovum card is made, limit application；Diamondback moth life cycle is short, and reproductive capacity is high, can expand kind rapidly in a short time
Group, obtains a large amount of ovum, but its ovum grain is small, causes the Trichogramma quality decline bred, and equally, Eggs of Diamondback Moth is not easy to make
Into ovum card, standardization is formed；The egg laying amount of prodenia litura is big, the ovum card of standard also can easily be made, but Trichogramma is posted
It can not complete to develop after life.Therefore it is anti-by the use of trichogramma as rice terrace biology to screen a kind of high Hosts of production efficiency
The important foundation controlled.
Rice moth egg is the main Hosts for breeding Trichogramma at present, and generation of the usual rice moth at 26-28 DEG C is gone through
Phase is about 60 days, about 50 times of breeding rate.The generation time of beet armyworm at the same temperature about 23 days, breeding rate is about
100, then the breeding rate equally in 60 days reaches ten tens of thousands of times；The significantly larger than reproductive efficiency of rice moth.Raised through scale
Beet armyworm cost accounting is supported, 10,000 pupa costs are 100 yuan or so, and 10,000 pupas can be with numerous honeybee 2,000,000 (with averagely every pupa production
The Granule Computing of ovum 200) numerous honeybee cost is added, no more than 150 yuan.So the cost of every ten thousand honeybees is significantly less than rice moth less than 1 yuan
Cost.Using the present processes, generation more Trichogramma can be bred, population does not degenerate, and R0 is maintained at more than 10 times, so as to
Make merchandized handling Trichogramma, and large-area applications are possibly realized in the biological control of the snout moth's larva of rice.
Specific description of embodiments of the present invention above is not intended to limit the present invention, and those skilled in the art can be according to this
Invention is variously modified or deformed, and without departing from the spirit of the present invention, all should belong to the models of appended claims of the present invention
Enclose.
Claims (25)
1. a kind of artificial breeding production method of Trichogramma, wherein, it the described method comprises the following steps：
1) Beet Armyworm Eggs are produced；
2) Trichogramma is bred using Beet Armyworm Eggs；
Wherein, the step 1) is realized by the method comprised the following steps：
A) Beet Armyworm Eggs that selection will hatch are put into hatching house, are hatched 2-3 days；
B) after hatching, regrowth 4-6 days, larva is transferred to the raising of larva raising room, and adds feed, the larva raising phase is
10-15 days；
C) pupate after completing at least one day, by the pupa of beet armyworm, sterilize 20-40 minutes using 4% formalin, dry；Its
Middle beet armyworm pupa time is 5-7 days；
D) before pupa emergence, pupa is put into laying coop, wherein, 1-3 days early stage of Adult worms producting eggs, 4-6 days egg-laying season；
E) Beet Armyworm Eggs are collected.
Wherein in the step a), 2. feed according to the method for claim 1, is added in the larvae hatch room.
3. according to the method for claim 1, wherein in the step a), the temperature of the hatching house is 24-30 DEG C, wet
Spend for 60%-70%.
4. according to the method for claim 3, wherein in the step a), the temperature of the hatching house is 27 ± 1 DEG C, wet
Spend for 65%.
5. according to the method for claim 1, wherein in the step b), the feed is suitable for lepidopteran insect larvae
Man-made feeds.
6. according to the method for claim 1, wherein in the step d), the temperature of the laying coop is 24-30 DEG C, wet
Spend for 60%-70%.
7. according to the method for claim 1, wherein in the step d), the temperature of the laying coop is 27 ± 1 DEG C, wet
Spend for 65%.
8. according to the method for claim 1, wherein, the Beet Armyworm Eggs that step e) is collected into can be used for conservation.
9. according to the method for claim 8, wherein, 20- will be sterilized with 4% formalin for the Beet Armyworm Eggs of conservation
40 minutes, then dry.
10. according to the method for claim 1, wherein, the Beet Armyworm Eggs that step e) is collected into can be used for breeding rice borer
Trichogramma；
Simple grain will be divided into for the Beet Armyworm Eggs for breeding Trichogramma, ovum card is made.
11. according to the method for claim 10, wherein, the every square centimeter of the ovum card has Beet Armyworm Eggs 380 ± 20
Grain ovum.
12. according to the method for claim 10, wherein, the Beet Armyworm Eggs are divided into simple grain using 40 mesh sieves.
13. according to the method for claim 10, wherein, the ovum card under ultraviolet using inactivating.
14. according to the method for claim 13, wherein, the inactivation time is 20-40 minutes.
15. according to the method for claim 1, wherein, the step 2) is realized by the method comprised the following steps：
I) in numerous honeybee room, 24-30 DEG C of keeping temperature, humidity 70%-90%, according to the kind honeybee of Trichogramma and beet armyworm
Ovum is than 1:5~15 breed, and are taken out after 1 day；
II) ovum for completing spawning process is put into culturing room, before cultivating to Trichogramma development to pupa, then stored.
16. the method according to claim 11, wherein, in the step I) in, numerous honeybee room temperature is maintained at 27 ± 1
DEG C, humidity about 80%.
17. the method according to claim 11, wherein, in the step I) in, numerous honeybee room keeps dark state.
18. the method according to claim 11, wherein, in the step II) in, the temperature of the culturing room is maintained at
24-30 DEG C, humidity 70%-90%.
19. according to the method for claim 15, wherein, in the step II) in, the culture room temperature is maintained at 27 ±
1 DEG C, humidity about 80%.
20. according to the method for claim 15, wherein, in the step II) in, it is described to save as being placed in 5-7 DEG C cold
Hide in case and store.
21. according to the method for claim 15, wherein, bred in the step 2) using Beet Armyworm Eggs card, then
Store ovum card；
Or the Trichogramma egg parasitoid that spawning process is completed in step 2) is prepared as ovum card.
22. according to the method for claim 21, wherein, the every square centimeter of Trichogramma egg parasitoid ovum card has rice
380 ± 20, snout moth's larva Trichogramma ovum.
23. according to the method for claim 21, wherein, the Trichogramma egg parasitoid ovum card under ultraviolet using going out
It is living.
24. according to the method for claim 23, wherein, the inactivation time is 20-40 minutes.
25. according to the method any one of claim 1-24, wherein, the Trichogramma egg parasitoid is in field adaptability
Before, in 27 ± 1 DEG C of temperature, humidity 70%-90% heating developments.
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| CN105532587B (en) * | 2016-03-13 | 2017-11-14 | 福建艳璇生物防治技术有限公司 | Mediterranean flour moth method for breeding and application |
| CN105875519B (en) * | 2016-06-08 | 2017-02-08 | 中国热带农业科学院环境与植物保护研究所 | Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts |
| CN106689063B (en) * | 2016-12-07 | 2020-03-17 | 中国科学院动物研究所 | Artificial breeding production method of trichogramma canker of rice borer |
| CN108056070B (en) * | 2018-01-16 | 2021-11-12 | 吉林农业大学 | Method for breeding trichogramma by using snowflake moth |
| CN110521666A (en) * | 2018-05-25 | 2019-12-03 | 田志伟 | A kind of human assistance mating system of Black-winged Stilt |
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| CN113349166B (en) * | 2021-07-23 | 2022-10-28 | 福建省农业科学院水稻研究所 | Method for breeding rice louse trichogramma in batches |
| CN113508713A (en) * | 2021-07-30 | 2021-10-19 | 中国农业科学院植物保护研究所 | Method for breeding trichogramma by using eggs of tuber moths of potatoes |
| CN114651793B (en) * | 2022-05-01 | 2023-04-18 | 中国医学科学院药用植物研究所云南分所 | Artificial breeding of trichogramma obscura and method for preventing and treating spodoptera frugiperda by using trichogramma obscura |
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| CN101564024B (en) * | 2009-04-21 | 2012-07-04 | 福建农林大学 | Technique for producing sugarcane Oophagous trichogrammae ovum piece |
| CN102113471B (en) * | 2011-02-22 | 2012-07-04 | 山东农业大学 | Artificial rearing method of larvae of beet armyworm |
| CN102823550B (en) * | 2012-09-19 | 2013-12-25 | 吉林农业大学 | Light environment control method for breeding trichogramma dendrolimi with oak silkworm eggs |
| CN103238568B (en) * | 2013-05-13 | 2015-04-22 | 广东省农业科学院植物保护研究所 | Trichogramma releasing solution and releasing method thereof |
| CN103444633B (en) * | 2013-08-21 | 2014-11-26 | 吉林农业大学 | Synergistic processing method for breeding trichogramma chilonis by using oak silkworm egg |
| CN103859210B (en) * | 2014-02-20 | 2015-10-28 | 广东省昆虫研究所 | A kind of trichogramma synthetic medium |
| CN103782969B (en) * | 2014-03-03 | 2015-08-19 | 广西壮族自治区林业科学研究院 | A kind of method of indoor propagation Trichogramma pintoi and rice moth culturing feed |
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