CN105875519B - Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts - Google Patents
Method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The invention discloses a method for efficiently reproducing brachymeria lasus by taking corcyra cephalonica pupae as hosts. According to the method, the cocooned corcyra cephalonica pupae which are refrigerated for 1.5 h at 5 DEG C are mainly taken as substitute hosts of the brachymeria lasus, the brachymeria lasus reproducing method is easy, convenient and rapid to oeprate, and the reproducing efficiency is high. Brachymeria lasus inoculation is conducted on the upper layer of a brachymeria lasus reproducing cage through the cocooned corcyra cephalonica pupae, the phototaxis of the brachymeria lasus is effectively utilized, the distance between the brachymeria lasus and a nutrient source and the hosts is shortened, the parasitism rate of the brachymeria lasus to the hosts and the feeding frequency are increased, the brachymeria lasus can be uninterruptedly and massively reproduced, therefore, scale production of the brachymeria lasus is achieved, and the obvious ecological benefit and economic benefit are achieved.
Description
Technical field
The present invention relates to agriculture and forestry injurious insect field of biological control, be related to a kind of rearing method of parasitic wasp and in particular to
A kind of method efficiently breeding vast leg chalcid fly using rice moth pupa as host.
Background technology
Coconut knits moth Opisina arenosella Walker also known as blackhead crawler belt worm, coconut palm moth moth, coconut wood moth, belongs to squama
Chi Mumu moth section, is a kind of weight of more than the 30 kind of babassus such as coconut of seriously causing harm, Chinese fan palm, Middle East nipa palm, betel nut, big yagua
Major pest.Mainly caused harm upwards from coconut lower floor old leaf with larva, take food mesophyll, when serious, only remain upper strata young leaves 3～4, its
Remaining blade is eaten up, thus causing the underproduction and quality decline, when serious, coconut palm fruit yield reduces more than 45%.
The taller and bigger babassu such as coconut is due to the more difficult enforcement of chemical prevention, and larva forms excrement in vacuum side of blade
Just with silk interweave burrow, harm of moving under water in burrow, mature larva spin under frass before pupating and cocoons, pupa positioned at frass with
In cocoon, chemopreventive effects are poor.Only rely on chemical prevention it is impossible to fundamentally prevent and kill off coconut to knit moth, this worm can be made repeatedly to cause harm.
The harm that moth knitted by coconut becomes the key issue of coconut industry.In order to more effectively control coconut to knit the harm of moth, reduce to ring
The pollution in border, rationally carry out coconut to knit the prevention and control of moth using parasitic wasp is current first-selected measure.
At home, applicant is found that the main parasitic honeybee that the chrysalis phase knitted by coconut is vast leg chalcid fly first
Brachymeria lasus Walker.Vast leg chalcid fly belongs to Hymenoptera, Chalcididae, thigh Nikol.This parasitic wasp is permissible
The nest cocoon of moth knitted by coconut of breaking by the teeth, knits chrysalis by ovipositing hosts and the lethal coconut of 2 kinds of modes of host-feeding.At 25 DEG C, vast leg
Chalcid fly Life of Adult 35.4 days, the filial generation quantity that queen bee all one's life is produced is 69 about, can be parasitic and take food about 87
Chrysalis knitted by coconut.The relevant report of domestic also not this parasitic wasp biological control up till now.
Therefore, solve manually the raising on a large scale of vast leg chalcid fly, be realize coconut knit moth, rice leaf roller, rice green caterpillar,
One of effective and crucial approach of many Species of Lepidopterous Insect Pests sustainable harnessing such as rice plant skipper.
Content of the invention
It is an object of the invention to provide a kind of method efficiently breeding vast leg chalcid fly using rice moth pupa as host, the party
Method mainly refrigerates the rice moth pupa with cocoon for the 1.5h as the Hosts of vast leg chalcid fly using at 5 DEG C, and numerous honeybee method is simple and efficient, numerous
Grow efficiency high.Carry out connecing honeybee on the upper strata of numerous honeybee cage with the rice moth pupa with cocoon, the phototaxis of effectively utilizes vast leg chalcid fly, shortens
The distance of vast leg chalcid fly and nutrient source and host, improves vast leg chalcid fly to the parasitic rate of host with the frequency that takes food,
Can amount reproduction vast leg chalcid fly incessantly, thus realizing the large-scale production of vast leg chalcid fly, there is obvious Ecological Effect
Benefit and economic benefit.
To achieve these goals, the technical scheme is that：There is provided vast as the efficient breeding of host using rice moth pupa
The method of leg chalcid fly, comprises the following steps：
(1) Hosts are raised：The thick feed of one layer of 1～1.5cm of paving in plastic casing；1 is uniformly spilt on feed～
1.5mL rice moth egg, then with sieving the free-and-easy one layer feed just covering ovum grain；Throw a feed within every 3 days after 15 days, input amount is children
The worm appetite of 3 days；After larvae development was to 30 days, spread two parallel nylon ropes on the surface of feed, rope two ends are exposed, and
When feeding intake thereafter, by described rope embedment material；After larva cocoons and pupates, described rope is together lifted together with cocoon block；
Take the rice moth pupa of rope upper band cocoon, standby；
(2) prepare numerous honeybee cage：Indoor in numerous honeybee, numerous honeybee cage is placed in top is provided with fluorescent tube and bottom is provided with wire netting
Foster worm frame on, described numerous honeybee cage is made up of nylon wire, and is provided with warp layer net, gauze at 2/3～3/4 height in numerous honeybee cage
It is fixed on numerous honeybee cage by fixture, gauze makes to be formed upper and lower two-layer in numerous honeybee cage, upper and lower two-layer communicates；
Because fluorescent tube is arranged above numerous honeybee cage, the light above numerous honeybee cage is stronger, simultaneously vast leg chalcid fly have very strong
Phototaxis, be gathered in cage top under the trapping of light above numerous honeybee cage；Numerous honeybee cage is set to upper and lower two-layer simultaneously.On
The layer distance away from numerous honeybee cage top for the gauze is little, and about 7.5～10cm, so that the vast leg chalcid fly being in cage top is frequent
Take food the mixed sugar liquid being positioned over upper strata and rice moth pupa with cocoon supplements the nutrients, improve parasitic wasp to band cocoon rice moth simultaneously
The parasitic rate of pupa；Lower floor is that vast leg chalcid fly provides sufficient activity space, significantly reduce vast leg chalcid fly susceptible can
Can property.
(3) obtain the band cocoon rice moth pupa of refrigeration：Rice moth pupa with cocoon is placed in refrigeration 1.5h at 5 DEG C, obtains the band of refrigeration
Cocoon rice moth pupa, standby；
(4) obtain the rice moth pupa with cocoon：With scissors one long 2～3mm cut on the cocoon of rice moth pupa, wide by 1～
The oval port of 1.5mm, makes rice moth pupa partial denudation out, obtains the rice moth pupa with cocoon, standby；
(5) gather vast leg chalcid fly：Knit moth in coconut to cause harm peak period, chrysalis will be knitted by the parasitic coconut of vast leg chalcid fly
Gather go back to interior, collect vast leg chalcid fly as honeybee kind introduces a collection；
(6) raise vast leg chalcid fly：
Rice moth pupa with cocoon, the band cocoon rice moth pupa of refrigeration are placed on a, the gauze on upper strata in numerous honeybee cage and speckles with
The cotton balls of the mixed sugar liquid of concentration 10%～25%, described mixed sugar liquid by weight 112 by sucrose pyroglutamate, plus
Distilled water diluting is made；Rice moth pupa with cocoon is 1 10～20 with the band cocoon rice moth pupa specific lobe of refrigeration；
B, vast leg chalcid fly is put in numerous honeybee cage, the sex ration of wherein vast leg chalcid fly is 13, and stocking density is：8～
10cm3/ head, controls 25 DEG C～27 DEG C of the temperature of numerous honeybee room, and relative humidity is 65%～85%, and lighting time is 12～15h；Extensively
Thigh chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days supplementary 100～200 vast leg chalcid flies into numerous honeybee cage, replacing in every 3 days speckles with mixed sugar liquid 1 time
The band cocoon rice moth pupa of cotton balls, the rice moth pupa with cocoon and refrigeration；Band cocoon rice by vast leg chalcid fly queen bee quantity and refrigeration
Chrysalis specific lobe is 17 honeybee worm ratio, divides, with cocoon rice moth pupa, the culture dish being placed on upper strata gauze in numerous honeybee cage by refrigerate
Interior, allow the rice moth pupa of vast leg chalcid fly nature parasitic strip cocoon；
D, by change after the rice moth pupa with cocoon and refrigeration band cocoon rice moth pupa take out put in conical flask, bottleneck
Seal up the nylon gauze of 100～200 mesh, tightened with rubber band, under the conditions of conical flask is positioned over 25 DEG C～27 DEG C of room temperature, ovum arrives
The development duration of adult is 15～18 days；
E, after the little bee eclosion of vast leg in conical flask, take off the nylon gauze of bottleneck, put in insect cage, insect cage
In be placed with the cotton balls speckling with mixed sugar liquid, be provided with fluorescent tube above insect cage, allow vast leg chalcid fly climb out of conical flask, concentrate on
It is used in insect cage breeding the next generation, or knit the lepidoptera pests such as moth for field release preventing and treating coconut.
Further, the feed ingredient in step (1) calculates by weight by 50 parts of corn flour, 25 parts of flour, wheat bran 20
Part, 3 parts of white granulated sugar, 2 parts of yeast are configured to.
Further, the stocking density that described step (6) raises vast leg chalcid fly is 9cm3/ head.
The method that the present invention efficiently breeds vast leg chalcid fly using rice moth pupa as host has following beneficial effect：
(1) present invention firstly discovers that can with rice moth pupa host's biological control vast leg chalcid fly as an alternative.Rice moth
Man-made feeds simplicity be easy to get, breeding technology is ripe, it is possible to achieve large-scale breeding.
(2) using the rice moth pupa host as an alternative with cocoon through refrigerating 1.5h at 5 DEG C, it breeds vast the present invention
It is suitable that chrysalis knitted by the effect of leg chalcid fly and natural host coconut.
(3) present invention passes through to supply vast leg chalcid fly parasitism through the band cocoon rice moth pupa in 5 DEG C of refrigeration 1.5h；Parasitic wasp
The parasitic rate with cocoon rice moth pupa through refrigeration is significantly higher than to the parasitic rate with cocoon rice moth pupa without refrigeration, shows simultaneously
Write higher than parasitic rate to the rice moth pupa with cocoon, eliminate the time-consuming rice moth pupa with cocoon is cut and after mouth, supply this and post
The parasitic tedious steps of raw honeybee；Simultaneously because rice moth is easily achieved extensive raising, it is obtained in that substantial amounts of rice moth pupa supply is wide
Thigh chalcid fly is parasitic, it is achieved thereby that the large-scale breeding of vast leg chalcid fly.
(4) instant invention overcomes when vast leg chalcid fly breeds coconut knit the natural hosts such as moth amount reproduction time-consuming,
Limited source, simultaneously natural foodstuff coconut leaf etc. limited it is impossible to meet the wilderness demand to host for this parasitic wasp of biological control
Problem.
(5) numerous honeybee cage of the present invention is set to upper and lower two-layer, and upper strata is put rice moth pupa and speckled with 10%～25% mixing with holding
The culture dish of the cotton balls of liquid glucose；Lower floor provides sufficient activity space for parasitic wasp, significantly reduces vast leg chalcid fly susceptible
Possibility.
(6) present invention is 1 10～20 using the band cocoon rice moth pupa of the rice moth pupa with cocoon, refrigeration by optimal specific lobe
It is applied in combination, the rice moth pupa with cocoon is mainly as supplying nutrient source vast leg chalcid fly, 5 DEG C of refrigeration 1.5h band cocoon rice moth pupa masters
Parasitic as the host vast leg chalcid fly of supply, the rice moth pupa after two kinds of different disposal is applied in combination it is ensured that biological control
This parasitic wasp.
(7) utensil that the present invention uses is very simple, only using numerous honeybee cage, culture dish, cotton, refrigerator, conical flask, supports worm
Cage etc., numerous honeybee efficiency high, there is wide industrialization prospect.
Brief description
Fig. 1 is that the numerous honeybee cage in the method that the present invention efficiently breeds vast leg chalcid fly using rice moth pupa as host is placed in foster worm
Structural representation on frame；
In figure：1st, fluorescent tube；2nd, support worm frame；3rd, numerous honeybee cage；4th, gauze；5th, wire netting；6th, sleeve；7th, zipper door.
Specific embodiment
In order to describe in detail the present invention using rice moth pupa as host efficiently breed the method for vast leg chalcid fly technology contents,
Structural feature, below in conjunction with embodiment and coordinate accompanying drawing to be described further.
Embodiment 1
(1) Hosts are raised：In plastic casing, (feed ingredient calculates by jade the thick feed of one layer of 1cm of paving by weight
50 parts of ground rice, 25 parts of flour, 20 parts of wheat bran, 3 parts of white granulated sugar, 2 parts of yeast are configured to)；1mL rice moth is uniformly spilt on feed
Ovum, then with sieving the free-and-easy one layer feed just covering ovum grain；Throw a feed within every 3 days after 15 days, input amount is the larva food of 3 days
Amount；After larvae development was to 30 days, spread two parallel nylon ropes on the surface of feed, rope two ends are exposed, and throw thereafter
When material, by described rope embedment material；After larva cocoons and pupates, described rope is together lifted together with cocoon block；Take rope upper band
The rice moth pupa of cocoon, standby；
(2) prepare numerous honeybee cage：As shown in figure 1, indoor in numerous honeybee, numerous honeybee cage 3 is placed in top and is provided with fluorescent tube 1 and bottom
It is provided with the foster worm frame 2 of wire netting 5, described numerous honeybee cage 3 is made up of nylon wire, and is provided with one at 2/3 height in numerous honeybee cage
Layer gauze 4,4 four angles of gauze are fixed on numerous honeybee cage 3 by fixture, and gauze 4 makes to be formed upper and lower two-layer in numerous honeybee cage, up and down
Two-layer communicates；
(3) obtain the band cocoon rice moth pupa of refrigeration：Rice moth pupa with cocoon is placed in refrigeration 1.5h at 5 DEG C, obtains the band of refrigeration
Cocoon rice moth pupa, standby；
(4) obtain the rice moth pupa with cocoon：With scissors, one long 2mm, the ellipse of wide 1mm are cut on the cocoon of rice moth pupa
Shape mouth, makes rice moth pupa partial denudation out, obtains the rice moth pupa with cocoon, standby；
(5) gather vast leg chalcid fly：Knit moth in coconut to cause harm peak period, chrysalis will be knitted by the parasitic coconut of vast leg chalcid fly
Gather go back to interior, collect vast leg chalcid fly as honeybee kind introduces a collection；
(6) raise vast leg chalcid fly：
Rice moth pupa with cocoon, the band cocoon rice moth pupa of refrigeration are placed on a, the gauze on upper strata in numerous honeybee cage and speckles with
The cotton balls of the mixed sugar liquid of concentration 10%, described mixed sugar liquid by weight 112 is by sucrose pyroglutamate, plus distilled water
Dilution is made；Rice moth pupa with cocoon is 1 10 with the band cocoon rice moth pupa specific lobe of refrigeration；
B, vast leg chalcid fly is put in numerous honeybee cage, the sex ration of wherein vast leg chalcid fly is 13, and stocking density is：
8cm3/ head, controls 25 DEG C of the temperature of numerous honeybee room, and relative humidity is 65%, and lighting time is 12h (to meet vast leg chalcid fly
Healthy development)；Vast leg chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days into numerous honeybee cage supplement 100 vast leg chalcid flies, every 3 days change speckle with for 1 time mixed sugar liquid cotton balls,
The band cocoon rice moth pupa of the rice moth pupa with cocoon and refrigeration；Band cocoon rice moth pupa head by vast leg chalcid fly queen bee quantity and refrigeration
Number, than honeybee worm ratios for 17, dividing with cocoon rice moth pupa of refrigeration is placed on the gauze on upper strata in numerous honeybee cage, allows vast leg chalcid fly
The rice moth pupa of natural parasitic strip cocoon；
D, by change after the rice moth pupa with cocoon and refrigeration band cocoon rice moth pupa take out put in conical flask, bottleneck
Seal up the nylon gauze of 100 mesh, tightened with rubber band, under the conditions of conical flask is positioned over 25 DEG C of room temperature, the growth of ovum to adult
Go through phase average out to 18 days；
E, after the little bee eclosion of vast leg in insect box, for breeding the next generation, or for field release preventing and treating coconut
Knit the lepidoptera pests such as moth.
Embodiment 2
(1) Hosts are raised：In plastic casing the thick feed of one layer of 1.5cm of paving (feed ingredient calculate by weight by
50 parts of corn flour, 25 parts of flour, 20 parts of wheat bran, 3 parts of white granulated sugar, 2 parts of yeast are configured to)；1.5mL rice is uniformly spilt on feed
Moth ovum, then with sieving the free-and-easy one layer feed just covering ovum grain；Throw a feed within every 3 days after 15 days, input amount is larva 3 days
Appetite；After larvae development was to 30 days, spread two parallel nylon ropes on the surface of feed, rope two ends are exposed, and thereafter
When feeding intake, by described rope embedment material；After larva cocoons and pupates, described rope is together lifted together with cocoon block；Take on rope
With the rice moth pupa of cocoon, standby；
(2) prepare numerous honeybee cage：As shown in figure 1, indoor in numerous honeybee, numerous honeybee cage 3 is placed in top and is provided with fluorescent tube 1 and bottom
It is provided with the foster worm frame 2 of wire netting 5, described numerous honeybee cage 3 is made up of nylon wire, and is provided with one at 3/4 height in numerous honeybee cage
Layer gauze 4,4 four jiaos of gauze is fixed on numerous honeybee cage 3 by fixture, and gauze 4 makes the upper and lower two-layer of formation in numerous honeybee cage, and upper and lower two
Layer communicates；
(3) obtain the band cocoon rice moth pupa of refrigeration：Rice moth pupa with cocoon is placed in refrigeration 1.5h at 5 DEG C, obtains the band of refrigeration
Cocoon rice moth pupa, standby；
(4) obtain the rice moth pupa with cocoon：With scissors, one long 3mm is cut on the cocoon of rice moth pupa, wide 1.5mm's is ellipse
Circular port, makes rice moth pupa partial denudation out, obtains the rice moth pupa with cocoon, standby；
(5) gather vast leg chalcid fly：Knit moth in coconut to cause harm peak period, chrysalis will be knitted by the parasitic coconut of vast leg chalcid fly
Gather go back to interior, collect vast leg chalcid fly as honeybee kind introduces a collection；
(6) raise vast leg chalcid fly：
Rice moth pupa with cocoon, the band cocoon rice moth pupa of refrigeration are placed on a, the gauze on upper strata in numerous honeybee cage and speckles with
The cotton balls of the mixed sugar liquid of concentration 25%, described mixed sugar liquid by weight 112 is by sucrose pyroglutamate, plus distilled water
Dilution is made；Rice moth pupa with cocoon is 1 20 with the band cocoon rice moth pupa specific lobe of refrigeration；
B, vast leg chalcid fly is put in numerous honeybee cage, the sex ration of wherein vast leg chalcid fly is 13, and stocking density is：
9cm3/ head, controls 27 DEG C of the temperature of numerous honeybee room, and relative humidity is 85%, and lighting time is 15h；Vast leg chalcid fly takes food mixing
Liquid glucose and rice moth pupa；
C, every 3 days into numerous honeybee cage supplement 200 vast leg chalcid flies, every 3 days change speckle with for 1 time mixed sugar liquid cotton balls,
The band cocoon rice moth pupa of the rice moth pupa with cocoon and refrigeration；Band cocoon rice moth pupa head by vast leg chalcid fly queen bee quantity and refrigeration
Number, than honeybee worm ratios for 17, dividing with cocoon rice moth pupa of refrigeration is placed on the gauze on upper strata in numerous honeybee cage, allows vast leg chalcid fly
The rice moth pupa of natural parasitic strip cocoon；
D, by change after the rice moth pupa with cocoon and refrigeration band cocoon rice moth pupa take out put in conical flask, bottleneck
Seal up the nylon gauze of 200 mesh, tightened with rubber band, under the conditions of conical flask is positioned over 27 DEG C of room temperature, the growth of ovum to adult
Go through phase average out to 15 days；
E, after the little bee eclosion of vast leg in insect box, for breeding the next generation, or for field release preventing and treating coconut
Knit the lepidoptera pests such as moth.
Embodiment 3
(1) Hosts are raised：In plastic casing, (feed ingredient calculates the thick feed of one layer of 1～1.5cm of paving by weight
It is configured to by 50 parts of corn flour, 25 parts of flour, 20 parts of wheat bran, 3 parts of white granulated sugar, 2 parts of yeast)；1.3mL is uniformly spilt on feed
Rice moth egg, then with sieving the free-and-easy one layer feed just covering ovum grain；Throw a feed within every 3 days after 15 days, input amount is larva 3 days
Appetite；After larvae development was to 30 days, spread two parallel nylon ropes on the surface of feed, rope two ends are exposed, and here
After when feed intake, by described rope embedment material；After larva cocoons and pupates, described rope is together lifted together with cocoon block；Take rope
The rice moth pupa of upper band cocoon, standby；
(2) prepare numerous honeybee cage：As shown in figure 1, indoor in numerous honeybee, numerous honeybee cage 3 is placed in top and is provided with fluorescent tube 1 and bottom
It is provided with the foster worm frame 2 of wire netting 5, described numerous honeybee cage 3 is made up of nylon wire, and is provided with one at 3/4 height in numerous honeybee cage
Layer gauze 4,4 four angles of gauze are fixed on numerous honeybee cage 3 by fixture, and gauze 4 makes to be formed upper and lower two-layer in numerous honeybee cage, up and down
Two-layer communicates；
(3) obtain the band cocoon rice moth pupa of refrigeration：Rice moth pupa with cocoon is placed in refrigeration 1.5h at 5 DEG C, obtains the band of refrigeration
Cocoon rice moth pupa, standby；
(4) obtain the rice moth pupa with cocoon：With scissors, one long 2.5mm is cut on the cocoon of rice moth pupa, wide 1.3mm's
Oval port, makes rice moth pupa partial denudation out, obtains the rice moth pupa with cocoon, standby；
(5) gather vast leg chalcid fly：Knit moth in coconut to cause harm peak period, chrysalis will be knitted by the parasitic coconut of vast leg chalcid fly
Gather go back to interior, collect vast leg chalcid fly as honeybee kind introduces a collection；
(6) raise vast leg chalcid fly：
Rice moth pupa with cocoon, the band cocoon rice moth pupa of refrigeration are placed on a, the gauze on upper strata in numerous honeybee cage and speckles with
The cotton balls of the mixed sugar liquid of concentration 20%, described mixed sugar liquid by weight 112 is by sucrose pyroglutamate, plus distilled water
Dilution is made；Rice moth pupa with cocoon is 1 15 with the band cocoon rice moth pupa specific lobe of refrigeration；
B, vast leg chalcid fly is put in numerous honeybee cage, the sex ration of wherein vast leg chalcid fly is 13, and stocking density is：
9cm3/ head, controls 26 DEG C of the temperature of numerous honeybee room, and relative humidity is 75%, and lighting time is 14h；Vast leg chalcid fly takes food mixing
Liquid glucose and rice moth pupa；
C, every 3 days into numerous honeybee cage supplement 150 vast leg chalcid flies, every 3 days change speckle with for 1 time mixed sugar liquid cotton balls,
The band cocoon rice moth pupa of the rice moth pupa with cocoon and refrigeration；Band cocoon rice moth pupa head by vast leg chalcid fly queen bee quantity and refrigeration
Number, than honeybee worm ratios for 17, dividing with cocoon rice moth pupa of refrigeration is placed on the gauze on upper strata in numerous honeybee cage, allows vast leg chalcid fly
The rice moth pupa of natural parasitic strip cocoon；
D, by change after the rice moth pupa with cocoon and refrigeration band cocoon rice moth pupa take out put in conical flask, bottleneck
Seal up the nylon gauze of 150 mesh, tightened with rubber band, under the conditions of conical flask is positioned over 26 DEG C of room temperature, the growth of ovum to adult
Go through phase average out to 17 days；
E, after the little bee eclosion of vast leg in insect box, for breeding the next generation, or for field release preventing and treating coconut
Knit the lepidoptera pests such as moth.
Choose the rice moth cocoon containing rice moth pupa as host, judge the standard whether larva inside rice moth cocoon has pupated
Be hardness is bigger cocoon in the explanation of rice moth larva pupated, and the rice moth larva inside softer rice moth cocoon does not also pupate.
As shown in figure 1, described numerous honeybee cage is provided with sleeve 6, sleeve barrel dliameter is 12～14cm, and length is 30～40cm, if
Put the top before cage.Sleeve is the little opening of cage, when supplementing or changing mixed sugar liquid, put into or take out rice moth pupa
The escape of vast leg chalcid fly can be prevented.
Zipper door 7, the high 20～25cm of zipper door, the wide 15～20cm in bottom are additionally provided with described numerous honeybee cage.In numerous honeybee cage
Above open a semi-circular door from the bottom up, be the zip mode big opening of cage, be provided with two slider of zipper fasteners, stitch in semi-circular door
It is formed with described sleeve.Zipper door is easy to put into parasitic wasp, host into cage, takes out host, dead parasitic wasp in cleaning cage
Operate with the cleaning of cage etc..
The described rice moth pupa with cocoon is to cut a long 2～3mm on rice moth cocoon with scissors, and wide 1～1.5mm's is ellipse
Circular port, makes rice moth pupa partial denudation out, is mainly supplied with vast leg chalcid fly and takes food host's body fluid extra-nutrition.
Rice moth pupa with cocoon is refrigerated at 5 DEG C and after 1.5h, supplies vast leg chalcid fly parasitism, K cryogenic treatment makes to be in cocoon
Rice moth pupa mobility reduce, parasitic wasp can consume minimum muscle power in the case of host's rice moth pupa is benumbed successfully, and then
Parasitism could be carried out to host, make vast leg chalcid fly that the parasitic rate of rice moth pupa is greatly improved.
Process of the test：
1st, the Choice tests to different days rice moth pupa for the vast leg chalcid fly
The little bee imago of vast leg 40 of 7 ages in days (sex ration is 1 3) is introduced numerous honeybee cage, will be placed with 15 1,2,3,4,
5th, on the radial gauze being positioned over numerous honeybee cage upper strata of the culture dish of 6,7,8,9,10,11 age in days rice moth pupas, to speckle with 20%
The cotton balls of mixed sugar liquid supplements the nutrients, parasitic 24h, and each process is repeated 3 times.Count vast leg chalcid fly to not to parasitic bee eclosion
Parasitic rate with age in days rice moth pupa.
The parasitic rate to different days rice moth pupa for the vast leg chalcid fly of table 1
Concrete outcome as shown in table 1, from table 1, vast leg chalcid fly can parasitic 1～10 age in days rice moth pupa, can not post
The rice moth pupa (parasitic rate is 0%) of raw 11 ages in days.Therefore, in order to avoid the age in days of rice moth pupa is more than 10 ages in days, simultaneously because rice
Moth mature larva can spin and cocoon, and the larvae pupation time is irregular, answer every 10 days from the rice moth culture medium being in the stage of pupating
Choose the rice moth pupa with cocoon for vast leg chalcid fly parasitism.
2nd, vast leg chalcid fly is tested to the parasitic rate of different disposal rice moth pupa
The little bee imago of vast leg 40 (sex ration is 1 3) of 7 ages in days is introduced in vial, puts into 30 different disposal
Rice moth pupa：Pupa with cocoon, the pupa with cocoon (with scissors one long 3mm cut on rice moth cocoon, the oval port of wide 1mm,
Make rice moth pupa partial denudation out), 5 DEG C refrigeration 0.5h with cocoon pupa, 5 DEG C refrigeration 1h with cocoon pupa, 5 DEG C refrigeration 1.5h band
Cocoon pupa, the band cocoon pupa of 5 DEG C of refrigeration 2h, are supplemented the nutrients with the cotton balls speckling with 20% mixed sugar liquid, parasitic 24h, each process repetition 3
Secondary.Count the parasitic rate to different disposal rice moth pupa for the vast leg chalcid fly to parasitic bee eclosion.
The parasitic rate to different disposal rice moth pupa for the vast leg chalcid fly of table 2
As shown in table 2, from table 2, vast leg chalcid fly is to the parasitic rate with cocoon pupa in 5 DEG C of refrigeration 1.5h for concrete outcome
Highest, is 90.01%, is significantly higher than to the parasitic rate with cocoon pupa and the pupa with cocoon in 5 DEG C of refrigerations 1h, 2h, 0.5h
(76.35%, 74.00%, 72.11%, 71.16%), the band cocoon rice without refrigeration of vast leg chalcid fly pair as can be known from the results
The parasitic rate of chrysalis is relatively low to be 43.47%, and by the rice moth pupa with cocoon through this parasitism can be significantly improved in 5 DEG C of refrigeration 1.5h
The parasitic rate (90.01%) to rice moth pupa for the honeybee.This parasitic wasp to the parasitic rate with cocoon rice moth pupa cutting mouth through scissors is
71.16%.Rice moth pupa with cocoon is refrigerated at 5 DEG C and after 1.5h, directly feeds vast leg chalcid fly parasitism, its parasitic rate is significantly high
In the parasitic rate to the pupa with cocoon, eliminate the time-consuming rice moth pupa with cocoon is cut and after mouth, supply this parasitic wasp parasitism
Tedious steps.Meanwhile, the present invention is 1 10 using the band cocoon rice moth pupa of the rice moth pupa with cocoon, refrigeration by optimal specific lobe
～20 are applied in combination, and the rice moth pupa with cocoon is mainly as supplying nutrient source vast leg chalcid fly, 5 DEG C of refrigeration 1.5h band cocoon rice moths
Pupa is parasitic mainly as the vast leg chalcid fly of host's supply, and the rice moth pupa after two kinds of different disposal is applied in combination, it is achieved thereby that extensively
The biological control of thigh chalcid fly.
3rd, the different impacts to vast leg chalcid fly reproductive capacity for the stocking density
Indoor in numerous honeybee, numerous honeybee cage is placed on the foster worm frame being provided with fluorescent tube and wire netting, puts into wide in numerous honeybee cage
Thigh chalcid fly 800,1000,1200 (sex ration is 1 3), supplements 100 vast leg chalcid flies in every 3 days into cage；Into cage
The cotton balls speckling with 20% mixed sugar liquid (sucrose pyroglutamate=1 12, plus the mixed liquor of distilled water diluting) is put on upper strata
Culture dish and 100 rice moth pupas with cocoon allow this parasitic wasp take food mixed sugar liquid and rice moth pupa to supplement the nutrients.Every 3 days
Supplement 100 vast leg chalcid flies into numerous honeybee cage, change cotton balls, the rice moth pupa with cocoon speckling with mixed sugar liquid for 1 time within every 3 days
And the band cocoon rice moth pupa of refrigeration；The honeybee worm being 17 with cocoon rice moth pupa specific lobe by vast leg chalcid fly queen bee quantity and refrigeration
Ratio dividing with cocoon rice moth pupa of refrigeration is placed on the culture dish of upper strata gauze in numerous honeybee cage, makes vast leg chalcid fly naturally parasitic
Rice moth pupa with cocoon；Taking out this batch of rice moth pupa and the rice moth pupa with cocoon afterwards within 3 days puts in conical flask, bottleneck rubber
Muscle seals up nylon gauze；More changing to the new rice moth pupa with cocoon in 5 DEG C of refrigeration 1.5h supplies this parasitic wasp parasitic, and changes to new
Rice moth pupa with cocoon takes food for parasitic wasp, circulates abovementioned steps.Treat the parasitic bee eclosion statistics bee yield in insect box.
Result of the test shows each numerous 800 vast leg chalcid fly of honeybee cage, and the bee yield of one month is 12900；Each
Numerous 1000 vast leg chalcid flies of honeybee cage, the bee yield of one month is 16800；Each numerous 1200 vast leg chalcid fly of honeybee cage,
The bee yield of one month is 16650.The above results show, each numerous honeybee cage should raise 1000 vast leg chalcid flies (according to
The volume of numerous honeybee cage is 9000cm3, show that optimal stocking density is 9cm3/ head), stocking density is low to be unfavorable for filling of numerous honeybee cage
Divide and utilize, the too high filial generation quantity that also can reduce every queen bee of stocking density.
4th, the breeding effect difference of individual layer numerous honeybee cage and double-deck numerous honeybee cage vast leg chalcid fly of raising
Indoor in numerous honeybee, numerous for individual layer honeybee cage (numerous honeybee cage is not provided to upper and lower two-layer) is placed in top and is provided with fluorescent tube and bottom
Portion is provided with the foster worm frame of wire netting, and (sex ration of vast leg chalcid fly is about to put into vast leg chalcid fly 1000 in numerous honeybee cage
For 1 3), supplement 100 vast leg chalcid flies into cage within every 3 days；Put into the bottom in cage and speckle with 20% mixed sugar liquid (sucrose
Pyroglutamate=1 12, plus the mixed liquor of distilled water diluting) the culture dish of cotton balls and 100 rice moth pupas with cocoon
(cut one on rice moth cocoon with scissors and be about 3mm, the oval port of wide about 1mm, make rice moth pupa partial denudation out), allows this
Parasitic wasp takes food mixed sugar liquid and rice moth pupa to supplement the nutrients；Supplement mixed sugar liquid daily, replacing in every 3 days speckles with mixed sugar 1 time
The cotton balls of liquid and the rice moth pupa with cocoon.Every 3 days by vast leg chalcid fly queen bee quantity and the rice moth pupa with cocoon than the honeybee for 17
Worm is divided than by the rice moth pupa with cocoon refrigerating 1.5h at 5 DEG C and is placed in culture dish, is placed on the gauze of bottom in cage, allows vast
Rice moth pupa with cocoon in this batch of rice moth pupa and cage is taken out after 3 days and puts into cone by the rice moth pupa of leg chalcid fly nature parasitic strip cocoon
In shape bottle, nylon gauze sealed up by bottleneck rubber band, more changes to the new rice moth pupa with cocoon in 5 DEG C of refrigeration 1.5h and supplies parasitic wasp
Parasitism, circulates abovementioned steps.
Result of the test shows 1000 vast leg chalcid flies of each individual layer numerous honeybee cage, and the bee yield of one month is 13145.
Test 3 (impacts to vast leg chalcid fly reproductive capacity of different stocking densities) from the present invention and understand that 1000, each double-deck numerous honeybee cage is wide
Thigh chalcid fly, the bee yield of one month is 16800.Therefore, numerous honeybee that double-deck numerous honeybee cage can improve vast leg chalcid fly is set
Amount.
5th, the impact supplementing the nutrients to vast leg chalcid fly Adult worms producting eggs amount
The firm vast leg chalcid fly 4 (sex ration is 1 3) sprouting wings is introduced in glass tube, daily supply speckles with 20% mixing
Liquid glucose (sucrose pyroglutamate=1 12, plus the mixed liquor of distilled water diluting), 20% sucrose, 20% glucose, 20% fruit
Sugar, the cotton mass of 20% honey, change to new rice moth pupa 25, the rice moth changed pupa are dissected under stereomicroscope, system
Meter egg laying amount, until adult is dead, each process is repeated 10 times.
The different impact supplementing the nutrients to vast leg chalcid fly Adult worms producting eggs amount of table 3
From table 3, different exogenous nutrition has a significant impact to the egg laying amount of vast leg chalcid fly.Mixed sugar liquid of the present invention
Egg laying amount highest, be 287 ± 20, be significantly higher than glucose, the egg laying amount of sucrose, fructose and honey treatment group.Therefore, should
Mixed sugar liquid (sucrose pyroglutamate=1 12, plus the mixed liquor of distilled water diluting) is selected to raise this parasitic wasp.
6th, rice moth pupa and coconut are knitted the vast leg chalcid fly that chrysalis breeds coconut are knitted with the parasitic ability of chrysalis and compare
Vial introduces 7 ages in days with rice moth pupa or coconut knit vast leg chalcid fly 40 that chrysalis breeds (male and female=
1 3), put into 50 coconuts and knit chrysalis, supplemented the nutrients with the cotton balls speckling with 20% mixed sugar liquid, parasitic 24h, it is repeated 3 times, wait to post
Their parasitic rate is counted after raw bee eclosion.
The vast leg chalcid fly of the different host's breeding of table 4 knits the parasitic rate of chrysalis to coconut
As known from Table 4 with the vast leg chalcid fly of rice moth pupa breeding coconut is knitted the parasitic rate of chrysalis for 94.00% ±
2.00%, the parasitic rate of chrysalis is knitted for 92.67% ± 2.31% with the vast leg chalcid fly that chrysalis breeding knitted by coconut to coconut, with rice
Chrysalis breeding vast leg chalcid fly and with coconut knit chrysalis breeding vast leg chalcid fly coconut is knitted with chrysalis parasitic rate difference not
Significantly, illustrate that the vast leg chalcid fly using the vast leg chalcid fly of rice moth pupa breeding and knitting chrysalis breeding with coconut is knitted in parasitic coconut
During moth, effect quite, controls coconut to knit when causing harm of moth breeding vast leg chalcid fly, can select rice moth pupa host as an alternative.
Vast leg chalcid fly is the highly advantageous natural enemy with being worth of a class.Present invention firstly provides with the band in 5 DEG C of refrigeration 1.5h
Host raises vast leg chalcid fly to the rice moth pupa of cocoon as an alternative, can this parasitic wasp of large-scale breeding.Realize the continuous of this parasitic wasp
Raise, on the one hand meet experimental study needs, on the other hand, the parasitic wasp that the breeding of scale obtains can apply to prevent and treat coconut palm
Son knits many Species of Lepidopterous Insect Pests such as moth, rice leaf roller, diamondback moth, mythimna separata, prodenia litura, rice green caterpillar, rice plant skipper, realizes to evil
The BIOLOGICAL CONTROL of worm.
Above disclosed be only presently preferred embodiments of the present invention, certainly the right of the present invention can not be limited with this
Scope, the equivalent variations therefore made according to the claims in the present invention, still fall within the scope that the present invention is covered.
Claims (3)
1. the method for vast leg chalcid fly is efficiently bred it is characterised in that comprising the following steps as host using rice moth pupa：
(1) Hosts are raised：The thick feed of one layer of 1～1.5cm of paving in plastic casing；1～1.5mL is uniformly spilt on feed
Rice moth egg, then with sieving the free-and-easy one layer feed just covering ovum grain；Throw a feed within every 3 days after 15 days, input amount is larva 3 days
Appetite；After larvae development was to 30 days, spread two parallel nylon ropes on the surface of feed, rope two ends are exposed, and here
After when feed intake, by described rope embedment material；After larva cocoons and pupates, described rope is together lifted together with cocoon block；Take rope
The rice moth pupa of upper band cocoon, standby；
(2) prepare numerous honeybee cage：Indoor in numerous honeybee, numerous honeybee cage is placed in top is provided with fluorescent tube and bottom is provided with supporting of wire netting
On worm frame, described numerous honeybee cage is made up of nylon wire, and is provided with warp layer net at 2/3～3/4 height in numerous honeybee cage, and gauze passes through
Fixture is fixed in numerous honeybee cage, and gauze makes to be formed upper and lower two-layer in numerous honeybee cage, and upper and lower two-layer communicates；
(3) obtain the band cocoon rice moth pupa of refrigeration：Rice moth pupa with cocoon is placed in refrigeration 1.5h at 5 DEG C, obtains the band cocoon rice of refrigeration
Chrysalis, standby；
(4) obtain the rice moth pupa with cocoon：With scissors, one long 2～3mm is cut on the cocoon of rice moth pupa, wide 1～1.5mm's
Oval port, makes rice moth pupa partial denudation out, obtains the rice moth pupa with cocoon, standby；
(5) gather vast leg chalcid fly：Knit moth in coconut to cause harm peak period, chrysalis collection will be knitted by the parasitic coconut of vast leg chalcid fly
Go back to interior, collect vast leg chalcid fly as honeybee kind introduces a collection；
(6) raise vast leg chalcid fly：
Rice moth pupa with cocoon, the band cocoon rice moth pupa of refrigeration are placed on a, the gauze on upper strata in numerous honeybee cage and speckles with concentration
The cotton balls of 10%～25% mixed sugar liquid, described mixed sugar liquid by weight 112 is by sucrose pyroglutamate, plus distillation
Water dilution is made；Rice moth pupa with cocoon is 1 10～20 with the band cocoon rice moth pupa specific lobe of refrigeration；
B, vast leg chalcid fly is put in numerous honeybee cage, the sex ration of wherein vast leg chalcid fly is 13, and stocking density is：8～
10cm3/ head, controls 25 DEG C～27 DEG C of the temperature of numerous honeybee room, and relative humidity is 65%～85%, and lighting time is little for 12～15
When；Vast leg chalcid fly takes food mixed sugar liquid and rice moth pupa；
C, every 3 days into numerous honeybee cage supplement 100～200 vast leg chalcid flies, every 3 days change speckle with for 1 time mixed sugar liquid cotton balls,
The band cocoon rice moth pupa of the rice moth pupa with cocoon and refrigeration；Band cocoon rice moth pupa head by vast leg chalcid fly queen bee quantity and refrigeration
Number, than the honeybee worm ratio for 17, dividing with cocoon rice moth pupa of refrigeration is placed in the culture dish of upper strata gauze in numerous honeybee cage, allows vast
The rice moth pupa of leg chalcid fly nature parasitic strip cocoon；
D, by change after the rice moth pupa with cocoon and refrigeration band cocoon rice moth pupa take out put in conical flask, bottleneck is sealed up
The nylon gauze of 100～200 mesh, is tightened with rubber band, and under the conditions of conical flask is positioned over 25 DEG C～27 DEG C of room temperature, ovum is to adult
Development duration be 15～18 days；
E, after the little bee eclosion of vast leg in conical flask, take off the nylon gauze of bottleneck, put in insect cage, put in insect cage
It is equipped with the cotton balls speckling with mixed sugar liquid, above insect cage, is provided with fluorescent tube, allow vast leg chalcid fly climb out of conical flask, concentrate on and support worm
It is used in cage breeding the next generation, or for field release preventing and treating lepidoptera pest.
2. as claimed in claim 1 using rice moth pupa as host efficiently breed vast leg chalcid fly method it is characterised in that：Institute
State the feed ingredient in step (1) to calculate by weight by 50 parts of corn flour, 25 parts of flour, 20 parts of wheat bran, 3 parts of white granulated sugar, ferment
Female 2 parts are configured to.
3. as claimed in claim 1 using rice moth pupa as host efficiently breed vast leg chalcid fly method it is characterised in that：Institute
The stocking density stating step (6) the vast leg chalcid fly of raising is 9cm3/ head.
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