CN108522435A - A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp - Google Patents
A kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp Download PDFInfo
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- CN108522435A CN108522435A CN201810588655.3A CN201810588655A CN108522435A CN 108522435 A CN108522435 A CN 108522435A CN 201810588655 A CN201810588655 A CN 201810588655A CN 108522435 A CN108522435 A CN 108522435A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention belongs to biotechnologies, and in particular to a kind of device and parasitic ability evaluation method of detection the parasitism performance of parasitic wasp.A kind of device of detection the parasitism performance of parasitic wasp, including：Combine the parasitic wasp babinet and host's babinet of connection；The parasitic wasp top of the box is equipped with ventilation hole and feed hole；Host's babinet matches with the parasitic wasp babinet, divulges information on host's babinet at least faceplate panels, host's box roof is detachable.The present invention provides the device of detection the parasitism performance of parasitic wasp, which is combined type, simple in structure, low manufacture cost, easy to operation convenient for assembling, dismounting；And suitable for the detection of a variety of the parasitism performance of parasitic wasp and effect, strong applicability.Using the device provides the parasitism performance of parasitic wasp evaluation method, this method can fast and accurately test the parasitic ability of parasitic wasp, and the performance for parasitic wasp pest control effect provides foundation and technical guarantee, capture the technical problem of numerous bee quality monitoring and detection hardly possible.
Description
Technical field
The invention belongs to biotechnologies, and in particular to a kind of device and parasitic ability of detection the parasitism performance of parasitic wasp
Evaluation method.
Background technology
Parasitic wasp accounts for the 20%~25% of caste, is animal monoid that is most abundant on the earth and most evolving.Parasitic wasp is
The natural enemy of many agriculture and forestry injurious insects is the main force for ensureing Crop securify production.Utilize the green prevention and control of parasitic wasp pest control
Measure had become the important component of agriculture and forestry injurious insect prevention already.Many important natural enemy insects have been realized in western developed country
The natural enemy insect commodity of the scale and commercialization of production, the production of only Dutch KOPPERT companies have occupied most of Europe
Market is widely used in farmland, orchard, greenhouse and garden crop.In contrast, in natural enemy parasitic wasp use aspects, China is only
TrichogrammaTrichogrammaSpp., Zhou Shi nibbles chalcid flyChouioia cunea, Scleroderma Xiao et WuScleroderma guani、
Encarsia formosaEncarsia formosa, aphidius gifuensisAphidius gifuensisAnd Microplitis mediator (Haliday)Microplitis mediatorRaising technology formed scale.
Extensive artificial breeding parasitic wasp, and field is released into control the generation of pest, it is to carry out biology to prevent
One of most effective measure controlled.But all the time, there is many technical barriers for the Field information of parasitic wasp.Wherein, most it is worth
It must arouse attention, the monitoring and detection of numerous bee quality.Following reason can seriously affect the parasitic ability and pest controlling of parasitic wasp
Effect：One, the expansion in indoor long-term mostly generation is numerous, inevitably results in the degeneration of population, and if bee body becomes smaller, activity diffusivity weakens,
And then influence search and positioning of the parasitic wasp to host insect；Two, in the merchandized handling of parasitic wasp, to extend parasitic wasp
Shelf life mostly uses cryopreservation method, and prolonged cold stores the reabsorption or ovarian duct that may result in parasitic wasp ovum
Atrophy, degeneration eventually lead to completely losing for the parasitism performance of parasitic wasp；Even if three, parasitic wasp is female cannot lay eggs at bee, but
Can show very positive search, positioning and parasitizing behavior to host insect, and parasitizing behavior at this time be it is meaningless and
It is worth, thus has no pest control effect to say.
Above various adverse effects can seriously restrict the performance of parasitic wasp pest control effect.For this purpose, in the numerous bee of artificial room
Afterwards, especially before being released to Field information, it is desirable that we must make objective evaluation to the parasitic ability of the parasitic wasp of breeding,
Can this be the key for ensureing the essential link of parasitic wasp biocontrol effect, and the parasitic wasp of breeding being put into practical application
Place.
Currently, there are mainly three types of conventional parasitic wasp parasitic effects detection methods.When post-mortem method, i.e., by parasitic
The identification of pin main body internal excited state embryo or larva calculates parasitic rate.This method is time-consuming, laborious, it is desirable that higher professional knowledge
Background；Second is that method for breeding, from parasitism, until parasitoid larva spinning cocoons and pupates or turn into bee, by bring out bee cocoon or
Sprout wings into bee and just can determine that parasitic success.This method accuracy is high, but equally takes a lot of work, arduously, and the period is longer；Third, molecule reflects
Determine method.From sample collection, DNA extractions finally need PCR instrument to complete special expand to a series of operating process such as electrophoretic analysis
Increase the detection and sequence analysis of band.This method relative costs are higher, and more demanding to the operation of personnel, thus practicability by
To limitation.
To overcome drawbacks described above, Chinese patent application notification number CN107182950A（A kind of parasitic wasp parasitic rate measurement dress
It sets and assay method）A kind of parasitic wasp parasitic rate measurement device and assay method are disclosed, is provided a kind of by posting after parasitism
It is main to shift and replace simple and convenient and at low cost parasitic wasp parasitic rate measurement device and assay method.The parasitic rate of the invention
Measurement device is not easy to parasitic wasp and the raising and existence of host because parasitic wasp and host insect are placed in same container
The cleaning of environment, not manageability and control, meanwhile, the method for detection parasitic wasp parasitic rate is method for breeding, and test period is longer, consumption
When, effort, it is often more important that, existing detection device can not comprehensively, the parasitic ability of effective evaluation parasitic wasp.Therefore, for
The prior art still has further improved necessity.
Invention content
In order to overcome problems of the prior art, the present invention provides a kind of detachable, modular detection is parasitic
The method of the device of bee parasitic ability and the Fast Evaluation the parasitism performance of parasitic wasp established using the device, to be related evil
The biological control of worm lays the foundation.
To achieve the above object, technical scheme is as follows：
A kind of device of detection the parasitism performance of parasitic wasp, the device include：Combine the parasitic wasp babinet and host's babinet of connection；
The parasitic wasp babinet is used for the propagating and breeding of parasitic wasp, the casees such as cylinder, round platform, rectangle or square can be used
Body structure；Parasitic wasp box baseplate is detachable, consequently facilitating being connect with host's babinet；Parasitic wasp top of the box be equipped with ventilation hole and
The on the one hand ventilation for ensuring parasitic wasp babinet of feed hole, wherein ventilation hole, on the other hand, parasitic wasp can be imported by ventilation hole
Parasitic wasp babinet is for cultivating；Feed hole is for feeding feed needed for parasitic wasp into parasitic wasp babinet；
For Campoletis chlorideae（Campoletis chlorideae）Feeding for, may be configured as absorbing water in feed hole
The columnar material of property, such as it is set as the sliver being prepared by cotton, when feeding feed, mass concentration is put into out of feed hole
For 10% hydromel, the needs of Campoletis chlorideae extra-nutrition can be met after then being adsorbed by sliver；
The cylinder mating with parasitic wasp babinet, circle can be used for the feeding of host after parasitic wasp oviposition in host's babinet
The body structures such as platform, rectangle or square, host's babinet at least faceplate panels are equipped with ventilation hole, to ensure to post in babinet
Oxygen demand during main insect growth；Host's box roof is detachable, consequently facilitating being connect with parasitic wasp babinet.
When connection, snap can be used in the parasitic wasp babinet and host's babinet, screw is screwed on, overlaps or embedding
Enter the modes such as combination to be attached, but should be noted that it is ensured that is connected is solid and reliable, to prevent parasitic wasp or host elder brother
Worm escapes.
The parasitic wasp babinet and host's babinet specifically can be used transparent material and be prepared, it is preferred that use polyethylene
Material preparation forms, consequently facilitating observation babinet internal excited state or the growth and development situation by parasitic host.
Under preferable case, parasitic wasp babinet and host's babinet also can be used Web materials and are prepared, so that it is guaranteed that preferably
Performance of ventilating；But should be noted that need to ensure that reticular density is appropriate, to avoid insect escape, while do not influence parasitic wasp and
The activity of host.
The device of the detection the parasitism performance of parasitic wasp, The concrete specification aspect：Parasitic wasp babinet uses cylindrical structure, directly
Diameter 15~20 cm, high 20~30 cm；Host's babinet uses frustum cone structure, 15~20 cm of upper end diameter, lower end diameter 10~15
Cm, high 20~30 cm.
Using the parasitism performance of parasitic wasp evaluation method of the detection the parasitism performance of parasitic wasp detection device, to evaluate cotton boll
Worm tooth lip ichneumon wasp（Campoletis chlorideae）In armyworm（Mythimna separata）On parasitic ability for, should
Method specifically comprises the following steps：
（1）Campoletis chlorideae is fed in parasitic wasp babinet respectively and feeds armyworm in host's babinet, specifically：
In parasitic wasp babinet, queen bee and drone on the day of using the hydromel of 10% mass concentration as feed, feeding is sprouted wings are allowed to
1 ~ 3d of pangamy（It is preferred that the 2d that mates）；
With number of individuals gauge, queen bee:Drone=1:1~2；Environmental condition requires：25 ± 2 DEG C of temperature, relative humidity 60% ~ 70%,
Photoperiod 14L：10D.
In host's babinet, feed and 1~3 age armyworm larva are put into（It is preferred that 2 instar larvaes）In right amount, 2~4h of feeding or so
To adapt to environment, when feeding environmental condition require be：25 ± 2 DEG C of temperature, relative humidity 60% ~ 70%, photoperiod 14L：10D.
（2）Parasitic wasp carries out parasitism to host, specifically：
Parasitic wasp babinet and host's babinet are attached, while ensuring that Campoletis chlorideae can enter in parasitic wasp babinet
In host's babinet, then host is freely searched for by queen bee, is positioned and parasitic, 1 ~ 4h of parasitism or so；
When ghost operation, when being preferable over the morning 8 ~ 10（It is preferred that when 9）Start, because the mobility of bee at this time is strong, ovipositing hosts row
It is active；
With number of individuals gauge, queen bee:Armyworm=1:10~60（Preferably 1:40）；
In practical operation, for more accurate evaluation the parasitism performance of parasitic wasp, can single parasitic wasp individually evaluated；
（3）Parasitic ability is evaluated, specifically：
Wait for step（2）After middle parasitizing behavior, parasitic wasp babinet is detached with host's babinet, armyworm in host's babinet is continued
0.5 ~ 1h or so is cultivated, and whether carry out observation judgement armyworm parasitic；
It is as follows to observe determination method：Armyworm is by after parasitism, if occurring apparent black oblong object under its body wall, you can sentence
Breaking, it is parasitic；The main reason is that：After Campoletis chlorideae bee ovum develops about 10 min or so in host's armyworm body, body
Color just becomes black from milky, and the variation of this body colour is clear and legible under armyworm body wall.
Accordingly, can determine whether parasitic wasp whether ovipositing hosts, and calculate within the unit interval oviposition rates of the bee, Yi Jizong
Egg laying amount, to judge the parasitic ability of Campoletis chlorideae.
It further, can be by the parasitic wasp of evaluation to be tested according to above-mentioned steps（2）, step（3）Iterative method continuous parasitic 2
~3d；With more the presence or absence of its parasitic ability of accurate evaluation and size；It is repeatable parasitic the reason is that：Campoletis chlorideae is laid eggs
Rhythmicity is very strong, therefore hyperparasite can more its parasitic ability of accurate evaluation.
In general, if parasitic wasp egg laying amount is in daily 20 ovum/2 h or more, you can it is normal to be considered as parasitic ability
Excellent bee.
Further, according to single head host（Armyworm）The number of middle bee ovum quantity, also can determine whether that parasitic wasp whether there is
Parasitizing behavior.
Campoletis chlorideaeCampoletis chlorideae, belong to Hymenoptera Hymenoptera, Ichneumonidae
Ichneumonidae, world wide distribution.Be distributed widely in Yellow River basin and the Yangtze river basin in China, including Henan, Hebei,
The ground such as Liaoning, Shaanxi, Shandong, Shanxi, Anhui, Hubei, Hunan, Jiangsu, Jiangxi, Sichuan, Zhejiang, Yunnan.A bollworm tooth lip Ji
Nearly 30 Lepidopterous of bee energy parasitic the cause harm grains such as wheat, corn, cotton, tobacco, tomato and capsicum, economy and vegetable crop
Pest, including bollwormHelicoverpa armigera, oriental tobacco budwormH. assulta, armywormMythimna separata, tiltedly
Autographa sppSpodoptera litura, black cutwormAgrotis ypsilon, lopper wormMamestra brassicaeWith
Beet armywormSpodoptera exiguaDeng natural contral is with obvious effects, especially the parasitic rate to great economy pest bollworm
Reach as high as 80% or more.Campoletis chlorideae is apparent to the control action of various crop pests, has important exploitation value
Value, therefore carry out bee parasitic ability evaluation and the application of the bee is had a very important significance.
The device of detection the parasitism performance of parasitic wasp provided herein in actual use can be by parasitic wasp and host point
It does not raise, when needing detection, container combination is got up to use, make to connect that bee, to move worm operation extremely convenient, thus be applicable to a variety of
The detection of the parasitism performance of parasitic wasp and effect.
It is emphasized that when evaluating the parasitism performance of parasitic wasp in the application, one of main improvement is to use glutinous
Worm as host, the main reason is that：Armyworm has realized the raising of man-made feeds, can ensure that standardization, the scale of test worm, no
It needs as with needing time and effort consuming cultivating and growing host plant when other insects feed, thus more convenient using armyworm as host；
On the other hand, bollworm has serious autotomy habit, so that relevant evaluation result is difficult to the parasitism of actual response parasitic wasp
Ability, and armyworm autotomy habit is weak is not necessarily to the single head raising as bollworm by armyworm after parasitism, significantly reduce manpower,
Material resources, and more can accurately evaluate the parasitic ability of parasitic wasp.
In general, compared to the prior art, the beneficial effects of the invention are as follows：1. the present invention provides detection parasitic wasps to post
The detection device of life ability, the device are combined type, are convenient for assembling, dismounting, connect bee, shifting worm operation extremely facilitates, apparatus structure
Simply, low manufacture cost, easy to operation.The parasitic ability of a variety of parasitic wasps is can detect, it is applied widely；2. the device is utilized,
The present invention provides using armyworm as host, quality, that is, parasitic ability to parasitic wasp includes fecundity, search capability, pest controlling effect
Fruit accomplishes quickly and accurately to assess and detect, to fully ensure that pest control effect of the parasitic wasp to target pest；3. the present invention provides
The standard of the numerous bee quality of identification, i.e. parasitic wasp egg laying amount can be considered that parasitic ability is excellent in daily 20 ovum/2 h or more
Parasitic wasp.
Description of the drawings
Fig. 1 is in embodiment by parasitic armyworm aspect graph；
Fig. 2 is the structural schematic diagram that the parasitism performance of parasitic wasp device is detected described in embodiment；
In figure：1, parasitic wasp babinet, 2, host's babinet, 3, ventilation hole, 4, feed hole, 5, ventilation duct, 6, fodder tube, 7, sliver.
Specific implementation mode
With reference to embodiment, invention is further explained, it is clear that described embodiment is only the present invention
A part of the embodiment, instead of all the embodiments.
It should be noted that armyworm employed in following embodiments, is a kind of common insect, can directly be acquired and be obtained by field
, man-made feeds feeding culture also can be used；Parasitic wasp is by taking Campoletis chlorideae as an example herein.
Embodiment 1
If Fig. 2 shows, a kind of device of detection the parasitism performance of parasitic wasp, which includes：It combines the parasitic wasp babinet 1 of connection and posts
Main box 2；The parasitic wasp babinet 1 is cylinder, is used for the propagating and breeding of Campoletis chlorideae；1 bottom plate of parasitic wasp babinet
Detachably, consequently facilitating being connect with host's babinet 2；1 top of parasitic wasp babinet is equipped with ventilation hole 3 and feed hole 4, wherein ventilation hole
3 one side are used to ensure the ventilation of parasitic wasp babinet, and on the other hand, Campoletis chlorideae can import parasitic wasp by ventilation hole 3
Babinet 1 is for cultivating；Feed hole 4 is for feeding feed needed for Campoletis chlorideae into parasitic wasp babinet 1；Host's case
Body 2 is round platform, and for the feeding of armyworm after Campoletis chlorideae oviposition, at least faceplate panels of host's babinet 2 are equipped with logical
Air holes, to ensure the oxygen demand in babinet in armyworm growth course；2 top plate of host's babinet is detachable, consequently facilitating with parasitism
Beehive body 1 connects.
Ventilation hole 3 and feed hole 4 can be equipped with ventilation duct 5 and fodder tube 6 respectively, be can be used on ventilation duct 5 with several small
The spiral cover in hole covers ventilation hole 3, in case Campoletis chlorideae is escaped；The cotton extended straight down is fixed in fodder tube 6
Item 7 when feeding feed, puts into hydromel from feed hole 4 is interior, can meet Campoletis chlorideae after then being adsorbed by sliver 7
The needs of extra-nutrition.The parasitic wasp babinet 1 and host's babinet 2 are connected using embedded mode, when connection, it is ensured that connection it is steady
Gu reliable, to prevent Campoletis chlorideae or armyworm from escaping, the material of parasitic wasp babinet 1 and host's babinet 2 is polyethylene
Plastics.
Campoletis chlorideae is evaluated using the device of the detection the parasitism performance of parasitic wasp（Campoletis chlorideae）In armyworm（Mythimna separata）On parasitic ability, this method specifically comprises the following steps：
（1）Feeding Campoletis chlorideae and the feeding armyworm in host's babinet 2 in parasitic wasp babinet 1 respectively, specifically：
In parasitic wasp babinet 1, just emerged female bees and each 1 of drone are fed with the hydromel of 10% mass concentration, have been allowed to hand over
In bee-keeping box first emerged female bees and drone are pressed 1 with 2d, or in advance:After 1 proportional paired mating 2d, take out for trying queen bee
1 moves into parasitic wasp babinet 1；In host's babinet 2, feed and 2 age armyworm larvas 40 are put into, feeding 3h or so is to adapt to
Environment.The environmental condition of parasitic wasp babinet 1 and host's babinet 2 requires：25 ± 2 DEG C of temperature, relative humidity 60%~70%, light week
Phase 14L：10D, 1000~1500Lx of intensity of illumination.
（2）Parasitic wasp carries out parasitism to host, specifically：
Parasitic wasp babinet 1 and host's babinet 2 are attached, while ensuring that 1 internal excited state of parasitic wasp babinet can enter host
In babinet 2, then by queen bee to host freely parasitic, parasitic 2h or so, ghost operation when the morning 9；
（3）Parasitic ability is evaluated, specifically：
Wait for step（2）After middle parasitizing behavior, parasitic wasp babinet 1 is detached with host's babinet 2, to armyworm in host's babinet 2
Continue to cultivate 0.5h or so, microscopy under body formula anatomical lens to judge whether armyworm is parasitic, and calculates the bee within the unit interval
Oviposition rates and total egg laying amount, to judge the parasitic ability of parasitic wasp.
As shown in Figure 1 by parasitic armyworm form, black position is the bollworm tooth lip Ji colonized in armyworm body in figure
Bee bee ovum.
It can be by the Campoletis chlorideae of evaluation to be tested according to above-mentioned steps（2）, step（3）Iterative method is continuously parasitic
3d, with more the presence or absence of its parasitic ability of accurate evaluation and size.
Meanwhile according in single head armyworm bee ovum quantity number, also can determine whether that parasitic wasp whether there is superparasitization behavior.
Embodiment 2
To evaluate Campoletis chlorideae（Campoletis chlorideae）In armyworm（Mythimna separata）On
For parasitic ability, using the parasitism performance of parasitic wasp detection device designed by embodiment 1, inventor has carried out further evaluation.
Detailed process is briefly discussed below.Parasitic wasp babinet is shown in Fig. 21, host's babinet is shown in Fig. 22.
Compared with embodiment 1 evaluates the parasitism performance of parasitic wasp method, the present embodiment adjustment is as follows：
Step（1）There are queen bee 1, drone 1, pangamy 2d to put into 3 age armyworms in host's babinet in parasitic wasp babinet
Larva 10, feeding 2h or so is to adapt to environment；Step（2）The parasitic time is 1h, when ghost operation, when the morning 8；Step
Suddenly（3）After parasitic wasp babinet is detached with host's babinet, armyworm in host's babinet is continued to cultivate 1h or so；By bollworm tooth lip
Ichneumon wasp is according to above-mentioned steps（2）, step（3）The continuous parasitism 2d of iterative method.
Embodiment 3
Parasitic wasp babinet is shown in Fig. 21, host's babinet is shown in Fig. 22.
Compared with 1 evaluation method of embodiment, the present embodiment adjustment is as follows：
Step（1）There are queen bee 5, drone 10, pangamy 1d that 1 queen bee is then taken to be put in parasitic wasp in parasitic wasp babinet
In babinet；In host's babinet, 1 age armyworm larva 60 is put into, feeding 4h or so is to adapt to environment；Step（2）The parasitic time
For 4h, when ghost operation, when the morning 10；Step（3）After parasitic wasp babinet is detached with host's babinet, to host's babinet
Interior armyworm continues to cultivate 1h or so；By Campoletis chlorideae according to above-mentioned steps（2）, step（3）The continuous parasitism 3d of iterative method.
The conditions such as queen bee and drone use ratio, pangamy time are subject to the condition of embodiment 1, investigate bee（Cotton boll
Worm tooth lip ichneumon wasp）Worm（Armyworm）The influence of ratio access, parasitic time to Campoletis chlorideae parasitic ability.Using upper commentary
The continuous parasitism 3d of valence method, is repeated 20 times, under stereoscopic anatomical lens, has first seen whether black bee ovum through pin main body wall, with
This further dissects all hosts for examination as the foundation for identifying ovipositing hosts, calculates eggs on average amount, the results are shown in Table 1.
The eggs on average amount of 1 Campoletis chlorideae parasitism armyworm of table
| The parasitic time（h） | Bee, worm ratio | Eggs on average amount（It is a） |
| 1 | 1:10 | 11.6±1.9 |
| 1:15 | 13.4±2.3 | |
| 1:20 | 15.9±2.7 | |
| 2 | 1:15 | 15.3±2.4 |
| 1:20 | 18.2±2.6 | |
| 1:40 | 25.8±2.9 | |
| 4 | 1:20 | 19.3±3.2 |
| 1:40 | 28.7±3.1 | |
| 1:60 | 36.3±3.6 |
As can be seen from Table 1, the parasitic time is 2h, bee worm ratio is 1:When 40, Campoletis chlorideae eggs on average amount is
25.8.Consider that the parasitic time is 2h, bee worm ratio is 1 from time distribution and armyworm dosage and laying:It is optimal item when 40
Part obtains accordingly, within the parasitic time tested daily, averagely produces the h or more of 20 ovum/2 and can be considered that parasitic ability is normal
Excellent bee.In addition, with the extension of parasitic time, the parasitic egg laying amount of Campoletis chlorideae increases.But because the beekeeping equipment has one
Fixed distinguishes that host whether by parasitic ability, can prevent the generation of superparasitization behavior, therefore, it is necessary to be supplied to bee abundance
Host produces so that it will breed ripe bee ovum most possibly in ovary in host insect body, can the accurate judgement bee
Ovipositing hosts ability and parasitic effects.Bee worm is than 1:40 can most optimally realize this purpose with parasitic time 2h.According to grinding
Study carefully, the parasitic time is too short, then is that learning behavior consumes the regular hour to search, the positioning of host because of parasitic wasp, to shadow
Ring the accurate evaluation of its ovipositing hosts ability；And parasitic overlong time, on the one hand relatively more host insect to be provided, can increase
Feeding cost and the workload for connecing worm；On the other hand then because of the creeping of host, spread and causes to climb from host's babinet for trying insect
Go out into parasitic wasp babinet, when leading to follow-up microscopy, expends more times.
We obtained within the parasitic time tested daily as a result, averagely produced the h or more of 20 ovum/2 and can be considered parasitic
The normal excellent bee of ability.
Using apparatus and method of the present invention, professional technician can handle 500 queen bees for each person every day, can be to pre- after sampling
Quality, that is, the parasitic ability of parasitic wasp for discharging field includes fecundity, search capability, pest control effect, accomplish quickly to assess with
Detection, quick and precisely evaluates the parasitism performance of parasitic wasp, to fully ensure that pest controlling of the parasitic wasp to target pest of release
Effect.
Claims (10)
1. a kind of device of detection the parasitism performance of parasitic wasp, which is characterized in that including：Combine parasitic wasp babinet and the host of connection
Babinet；The parasitic wasp box baseplate is detachable, and parasitic wasp top of the box is equipped with ventilation hole and feed hole；Host's babinet with
The parasitic wasp babinet matches, and host's babinet at least faceplate panels are equipped with ventilation hole, and host's box roof is detachable.
2. detecting the device of the parasitism performance of parasitic wasp according to claim 1, which is characterized in that be equipped with water imbibition in feed hole
Columnar material.
3. detecting the device of the parasitism performance of parasitic wasp according to claim 2, which is characterized in that the water imbibition columnar material
For sliver, sliver is equipped with hydromel, and the mass concentration of hydromel is 10%.
4. detecting the device of the parasitism performance of parasitic wasp according to claim 1, which is characterized in that parasitic wasp babinet and host's case
Body combination connection mode be snap connection, screw is screwed on, overlap or be embedded in combination in one kind.
5. detecting the device of the parasitism performance of parasitic wasp according to claim 1, which is characterized in that parasitic wasp babinet and host's case
The material of body is transparent plastic.
6. a kind of the parasitism performance of parasitic wasp evaluation method, which is characterized in that include the following steps：
（1）Parasitic wasp is fed in parasitic wasp babinet respectively and feeds armyworm in host's babinet：In parasitic wasp babinet, with bee
Mulse feeds emerged female bees and drone as feed, is allowed to pangamy；In host's babinet, by feed and appropriate 1~3 age
Armyworm larva, which puts into host's babinet, feeds a period of time to adapt to environment；
（2）Parasitic wasp carries out host parasitic：By parasitic wasp babinet and host's babinet assembly and connection, while ensuring parasitic wasp babinet
Internal excited state can enter host's babinet in, then host armyworm is freely searched for by queen bee, position and parasitism；
（3）Parasitic ability is evaluated：Wait for step（2）After middle parasitizing behavior, by parasitic wasp babinet and host's babinet point
From, to armyworm in host's babinet continue culture a period of time, observation judgement armyworm it is whether parasitic.
7. the parasitism performance of parasitic wasp evaluation method according to claim 6, which is characterized in that the parasitic wasp babinet and host
Environment temperature in babinet is 25 ± 2 DEG C, and relative humidity is 60% ~ 70%.
8. the parasitism performance of parasitic wasp evaluation method according to claim 6, which is characterized in that step（1）In armyworm be 2 ages
Larva, queen bee:Drone=1:1~2.
9. the parasitism performance of parasitic wasp evaluation method according to claim 6, which is characterized in that step（2）Middle parasitic wasp is parasitic
Time is 1 ~ 4h, and ovipositing hosts behavior carries out when the morning 8 ~ 10.
10. the parasitism performance of parasitic wasp evaluation method according to claim 6, which is characterized in that step（2）In in quantitative terms,
Queen bee:Armyworm=1:10~60.
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