CN111838077A - T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof - Google Patents
T-shaped observation room with parasitic wasp parasitic rate enhanced by volatile allelochemicals and observation method thereof Download PDFInfo
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- CN111838077A CN111838077A CN201910382056.0A CN201910382056A CN111838077A CN 111838077 A CN111838077 A CN 111838077A CN 201910382056 A CN201910382056 A CN 201910382056A CN 111838077 A CN111838077 A CN 111838077A
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- 230000003071 parasitic effect Effects 0.000 title claims abstract description 23
- 239000003627 allelochemical Substances 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000002708 enhancing effect Effects 0.000 claims abstract description 12
- 241001481304 Vespoidea Species 0.000 claims abstract description 9
- 241001122767 Theaceae Species 0.000 claims description 58
- 241000257303 Hymenoptera Species 0.000 claims description 30
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 23
- 241001414720 Cicadellidae Species 0.000 claims description 20
- 235000013601 eggs Nutrition 0.000 claims description 20
- 239000002904 solvent Substances 0.000 claims description 20
- 150000001875 compounds Chemical class 0.000 claims description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 10
- 108010000912 Egg Proteins Proteins 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 10
- 210000004681 ovum Anatomy 0.000 claims description 10
- 238000005507 spraying Methods 0.000 claims description 10
- 241000238631 Hexapoda Species 0.000 claims description 8
- 241000931705 Cicada Species 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 239000003337 fertilizer Substances 0.000 claims description 5
- 239000011521 glass Substances 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 230000013011 mating Effects 0.000 claims description 5
- 230000017448 oviposition Effects 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241000245665 Taraxacum Species 0.000 claims description 2
- 241000256856 Vespidae Species 0.000 claims 2
- 230000024241 parasitism Effects 0.000 claims 1
- 239000007921 spray Substances 0.000 claims 1
- 238000011160 research Methods 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 2
- 230000006399 behavior Effects 0.000 description 5
- 230000003750 conditioning effect Effects 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
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- 238000012423 maintenance Methods 0.000 description 1
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- 241000894007 species Species 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a T-shaped observation chamber for enhancing parasitic rate of parasitic wasps by volatile allelochemicals and an observation method thereof. The method has simple test and convenient operation, and can well research and observe the influence of the allelochemicals on the aspect of enhancing the control of the parasitic natural enemies, thereby accelerating the theoretical scientific research and practical application and popularization of the allelochemicals.
Description
Technical Field
The invention relates to the field of biology, in particular to a T-shaped observation room with a parasitic rate enhanced by volatile allelochemicals and an observation method thereof.
Background
Research shows that whether insects search for host plants or natural enemies search for hosts, the insects have a fixed behavior sequence and are generally divided into stages of orientation, positioning, discovery, admission and the like, the process is called as a behavior reaction chain, certain chemical substances derived from the hosts, the host plants or the interaction of the hosts and the host plants play an important role in the behavior process, and the information chemical substances among the species are called as allelopathic compounds; the tea garden is a relatively stable ecosystem, pests cannot be controlled below the economic hazard level in production practice by simply depending on natural populations of parasitic bees, so that pest control efficiency of the parasitic bees is urgently needed to be improved by utilizing natural enemy enhancement technologies of insects, currently researched and ascertained regulation and control technologies comprise release enhancement, activation enhancement and retention enhancement, each technology cannot leave participation of other sensitive compounds, the chemical substances generate behavior stimulation such as attraction, obstruction, maintenance and the like in different environments, and the enhancement and control effects on the behaviors of target objects are completed according to specific mechanisms of the chemical substances;
however, the existing allelochemicals do not have good research and observation methods for enhancing the control of the parasitic natural enemies, and therefore, a T-shaped observation room and an observation method thereof are provided, wherein the parasitic rate of parasitic bees is enhanced by the volatile allelochemicals.
Disclosure of Invention
Based on the technical problems in the prior art, the invention provides a T-shaped observation chamber with a parasitic rate enhanced by volatile allelochemicals and an observation method thereof.
The T-shaped observation chamber with the parasitic rate enhanced by the volatile allelochemicals comprises a first observation chamber, a second observation chamber, a third observation chamber, a fourth observation chamber, a fifth observation chamber and a sixth observation chamber, wherein the second observation chamber is positioned between the first observation chamber and the third observation chamber, the fourth observation chamber is positioned on the side of the third observation chamber far away from the second observation chamber, the fifth observation chamber is positioned on the side of the fourth observation chamber, and the sixth observation chamber is positioned on the side of the fourth observation chamber far away from the fifth observation chamber.
An observation method for enhancing parasitic rate of parasitic wasps by volatile allelochemicals, comprising the following steps:
s1: preparing raw materials: the tea seedlings are multiple tea seedlings of the same age and all of 3 years, the growth vigor of the tea seedlings is uniform, the tea seedlings are clonal potted tea seedlings which are not damaged by diseases and insects, fertilizer and water management is carried out according to the same standard, multiple leafhoppers obtained by successive generation propagation in a greenhouse are prepared, multiple female bees obtained by successive generation propagation by using eggs of the leafhoppers as hosts in the greenhouse are prepared, and allelochemicals and normal hexane are purchased from regular suppliers.
S2: spawning: firstly removing leafhoppers and potted tea seedlings in the S1, putting the potted tea seedlings in a greenhouse, putting the leafhoppers on the potted tea seedlings to lay eggs for 24 hours, taking 3-5 branches, 1 bud and 4 leafy tea tips with eggs, taking 50 egg laying holes in each tip in total as a group, and taking 2 groups;
s3: and (3) environmental conditioning: the T-shaped observation room is placed in a special laboratory, a fluorescent lamp is arranged right above the T-shaped observation room, light rays are uniformly irradiated from top to bottom, the temperature of the laboratory is controlled to be 24-26 ℃ by a temperature and humidity controller, and the relative humidity is 60-80%.
S4: and (3) concentration adjustment: the taraxacum compound described in S1 was removed and n-hexane was used to prepare a concentration gradient (10)-2g/mL、10-4g/mL、10-6g/mL), filling the solvent A to be detected into a sprayer, and putting n-hexane into the sprayer to obtain a control solvent B;
s5: pretreatment: and uniformly spraying the solvent A to be detected in the S4 on one group of the tips of the tea with the ovum to obtain a treatment group C, and uniformly spraying the contrast solvent B on the other group of the tips of the tea with the ovum to obtain a treatment group D.
S6: releasing bees: taking 2 female bees in S1 and placing in a second observation chamber, taking 2 female bees in S1 and placing in a fourth observation chamber, placing the treatment group C in a fifth observation chamber, placing the treatment group D in a sixth observation chamber, repeating the operation for 2 times, then exchanging the positions of the treatment group C and the treatment group D, repeating the operation for 8 times in total, exchanging the positions for 2 times, placing the female bees in 12 hours, and taking out the treatment group C and the treatment group D.
S7: culturing: placing the treatment group C and the treatment group D taken out of the S6 into an illumination incubator for culturing for 5 days to respectively obtain a product E and a product F;
s8: counting: and (5) placing the product E and the product F in the S7 under a microscope, and stripping cicada eggs from young stems of tea tips to count the parasitized rate.
Preferably, the first observation chamber, the second observation chamber, the third observation chamber, the fourth observation chamber, the fifth observation chamber and the sixth observation chamber are all communicated.
Preferably, the fluorescent lamp in S3 is a 40W fluorescent lamp.
Preferably, the model of the temperature and humidity controller in S3 is TDK 0302.
Preferably, the sprayer in S4 is DJ-092.
Preferably, the female bees in the S1 are female bees which emerge for 24 hours and are obtained by mating in groups.
Preferably, the first observation room, the second observation room, the third observation room, the fourth observation room, the fifth observation room and the sixth observation room are all made of colorless organic glass, and the length and the width of the first observation room and the second observation room are both 20 cm.
The invention has the beneficial effects that: the method has simple test and convenient operation, and can well research and observe the influence of the allelochemicals on the aspect of enhancing the control of the parasitic natural enemies, thereby accelerating the theoretical scientific research and practical application and popularization of the allelochemicals.
Drawings
FIG. 1 is a schematic view of a T-shaped observation chamber according to the present invention.
In the figure: 1. a first observation room; 2. a second observation chamber; 3. a third observation room; 4. a fourth observation room; 5. a fifth observation room; 6. and a sixth observation chamber.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example one
The T-shaped observation chamber comprises a first observation chamber 1, a second observation chamber 2, a third observation chamber 3, a fourth observation chamber 4, a fifth observation chamber 5 and a sixth observation chamber 6, wherein the second observation chamber 2 is positioned between the first observation chamber 1 and the third observation chamber 3, the fourth observation chamber 4 is positioned on the side, away from the second observation chamber 2, of the third observation chamber 3, the fifth observation chamber 5 is positioned on the side, away from the fourth observation chamber 4, of the fifth observation chamber 4, and the sixth observation chamber 6 is positioned on the side, away from the fifth observation chamber 5, of the fourth observation chamber 4.
The embodiment provides an observation method for enhancing parasitic rate of parasitic wasps by using volatile allelochemicals, which comprises the following steps:
s1: preparing raw materials: the tea seedlings are multiple tea seedlings of the same age and all of 3 years, the growth vigor of the tea seedlings is uniform, the tea seedlings are clonal potted tea seedlings which are not damaged by diseases and insects, fertilizer and water management is carried out according to the same standard, multiple leafhoppers obtained by successive generation propagation in a greenhouse are prepared, multiple female bees obtained by successive generation propagation by using eggs of the leafhoppers as hosts in the greenhouse are prepared, and allelochemicals and normal hexane are purchased from regular suppliers.
S2: spawning: firstly removing the leafhoppers and potted tea seedlings in the S1, putting the potted tea seedlings in a greenhouse, placing the leafhoppers on the potted tea seedlings to lay eggs for 24 hours, taking 3-5 branches, 1 bud and 4 leaves with egg tea tips, taking 50 egg laying holes in each tip in total as a group, and taking 2 groups.
S3: and (3) environmental conditioning: the T-shaped observation room is placed in the greenhouse, a fluorescent lamp is arranged right above the T-shaped observation room, light is uniformly irradiated from top to bottom, the temperature in the greenhouse is controlled to be 25 ℃ by a temperature and humidity controller, and the relative humidity is 70%.
S4: and (3) concentration adjustment: the tarnish compound described in S1 was taken out and the concentration (10) was adjusted with n-hexane-2g/mL), and charging the solvent A to be tested into a sprayer, and charging n-hexane into the sprayer to obtain a control solvent B.
S5: pretreatment: and uniformly spraying the solvent A to be detected in the S4 on one group of the tips of the tea with the ovum to obtain a treatment group C, and uniformly spraying the contrast solvent B on the other group of the tips of the tea with the ovum to obtain a treatment group D.
S6: releasing bees: 2 female bees in S1 are taken and placed in the second observation chamber 2, 2 female bees in S1 are taken and prevented from being placed in the fourth observation chamber 4, the treatment group C is placed in the fifth observation chamber 5, the treatment group D is placed in the sixth observation chamber 6, the positions of the treatment group C and the treatment group D are changed after 2 times of repeated operation, the operation is repeated for 8 times in total, the positions are changed for 2 times, and the treatment group C and the treatment group D are taken out after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group C and the treatment group D taken out of the S6 into an illumination incubator for culturing for 5 days to respectively obtain a product E and a product F;
s8: counting: and (5) placing the product E and the product F in the S7 under a microscope, and stripping cicada eggs from young stems of tea tips to count the parasitized rate.
In this embodiment, the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 are all communicated, the fluorescent lamp in S3 is a 40W fluorescent lamp, the temperature and humidity controller in S3 is TDK0302, the sprayer in S4 is DJ-092, the female bee in S1 is a female bee which is allowed to emerge for 24 hours and is obtained by mating groups, and the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 are all made of colorless organic glass and have a length and a width of 20 cm.
Example two
The T-shaped observation chamber comprises a first observation chamber 1, a second observation chamber 2, a third observation chamber 3, a fourth observation chamber 4, a fifth observation chamber 5 and a sixth observation chamber 6, wherein the second observation chamber 2 is positioned between the first observation chamber 1 and the third observation chamber 3, the fourth observation chamber 4 is positioned on the side, away from the second observation chamber 2, of the third observation chamber 3, the fifth observation chamber 5 is positioned on the side, away from the fourth observation chamber 4, of the fifth observation chamber 4, and the sixth observation chamber 6 is positioned on the side, away from the fifth observation chamber 5, of the fourth observation chamber 4.
The embodiment provides an observation method for enhancing parasitic rate of parasitic wasps by using volatile allelochemicals, which comprises the following steps:
s1: preparing raw materials: the tea seedlings are multiple tea seedlings of the same age and all of 3 years, the growth vigor of the tea seedlings is uniform, the tea seedlings are clonal potted tea seedlings which are not damaged by diseases and insects, fertilizer and water management is carried out according to the same standard, multiple leafhoppers obtained by successive generation propagation in a greenhouse are prepared, multiple female bees obtained by successive generation propagation by using eggs of the leafhoppers as hosts in the greenhouse are prepared, and allelochemicals and normal hexane are purchased from regular suppliers.
S2: spawning: firstly removing leafhoppers and tea seedling pot culture in S1, picking tea seedling pots in a greenhouse, placing the leafhoppers on the tea seedling pot culture to lay eggs for 24 hours, taking 3-5 branches of 1 bud and 4 leaves with egg tea tips, taking 50 egg laying holes in each tip as a group, and taking 2 groups;
s3: and (3) environmental conditioning: the T-shaped observation room is placed in a special laboratory, a fluorescent lamp is arranged right above the T-shaped observation room, light rays are uniformly irradiated from top to bottom, the temperature of the laboratory is controlled to be 25 ℃ by a temperature and humidity controller, and the relative humidity is 70%.
S4: and (3) concentration adjustment: the tarnish compound described in S1 was taken out and the concentration (10) was adjusted with n-hexane -4g/mL), and charging the solvent A to be tested into a sprayer, and charging n-hexane into the sprayer to obtain a control solvent B.
S5: pretreatment: and uniformly spraying the solvent A to be detected in the S4 on one group of the tips of the tea with the ovum to obtain a treatment group C, and uniformly spraying the contrast solvent B on the other group of the tips of the tea with the ovum to obtain a treatment group D.
S6: releasing bees: 2 female bees in S1 are taken and placed in the second observation chamber 2, 2 female bees in S1 are taken and prevented from being placed in the fourth observation chamber 4, the treatment group C is placed in the fifth observation chamber 5, the treatment group D is placed in the sixth observation chamber 6, the positions of the treatment group C and the treatment group D are changed after 2 times of repeated operation, the operation is repeated for 8 times in total, the positions are changed for 2 times, and the treatment group C and the treatment group D are taken out after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group C and the treatment group D taken out of the S6 into an illumination incubator for culturing for 5 days to respectively obtain a product E and a product F;
s8: counting: and (5) placing the product E and the product F in the S7 under a microscope, and stripping cicada eggs from young stems of tea tips to count the parasitized rate.
In this embodiment, the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 are all communicated, the fluorescent lamp in S3 is a 40W fluorescent lamp, the temperature and humidity controller in S3 is TDK0302, the sprayer in S4 is DJ-092, the female bee in S1 is a female bee which is allowed to emerge for 24 hours and is obtained by mating groups, and the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 are all made of colorless organic glass and have a length and a width of 20 cm.
EXAMPLE III
The T-shaped observation chamber comprises a first observation chamber 1, a second observation chamber 2, a third observation chamber 3, a fourth observation chamber 4, a fifth observation chamber 5 and a sixth observation chamber 6, wherein the second observation chamber 2 is positioned between the first observation chamber 1 and the third observation chamber 3, the fourth observation chamber 4 is positioned on the side, away from the second observation chamber 2, of the third observation chamber 3, the fifth observation chamber 5 is positioned on the side, away from the fourth observation chamber 4, of the fifth observation chamber 4, and the sixth observation chamber 6 is positioned on the side, away from the fifth observation chamber 5, of the fourth observation chamber 4.
The embodiment provides an observation method for enhancing parasitic rate of parasitic wasps by using volatile allelochemicals, which comprises the following steps:
s1: preparing raw materials: the tea seedlings are multiple tea seedlings of the same age and all of 3 years, the growth vigor of the tea seedlings is uniform, the tea seedlings are clonal potted tea seedlings which are not damaged by diseases and insects, fertilizer and water management is carried out according to the same standard, multiple leafhoppers obtained by successive generation propagation in a greenhouse are prepared, multiple female bees obtained by successive generation propagation by using eggs of the leafhoppers as hosts in the greenhouse are prepared, and allelochemicals and normal hexane are purchased from regular suppliers.
S2: spawning: firstly removing leafhoppers and potted tea seedlings in the S1, putting the potted tea seedlings in a greenhouse, putting the leafhoppers on the potted tea seedlings to lay eggs for 24 hours, taking 3-5 branches, 1 bud and 4 leafy tea tips with eggs, taking 50 egg laying holes in each tip in total as a group, and taking 2 groups;
s3: and (3) environmental conditioning: the T-shaped observation room is placed in a special laboratory, a fluorescent lamp is arranged right above the T-shaped observation room, light rays are uniformly irradiated from top to bottom, the temperature of the laboratory is controlled to be 25 ℃ by a temperature and humidity controller, and the relative humidity is 70%.
S4: and (3) concentration adjustment: the tarnish compound described in S1 was taken out and the concentration (10) was adjusted with n-hexane-6g/mL), and charging the solvent A to be tested into a sprayer, and charging n-hexane into the sprayer to obtain a control solvent B.
S5: pretreatment: and uniformly spraying the solvent A to be detected in the S4 on one group of the tips of the tea with the ovum to obtain a treatment group C, and uniformly spraying the contrast solvent B on the other group of the tips of the tea with the ovum to obtain a treatment group D.
S6: releasing bees: 2 female bees in S1 are taken and placed in the second observation chamber 2, 2 female bees in S1 are taken and prevented from being placed in the fourth observation chamber 4, the treatment group C is placed in the fifth observation chamber 5, the treatment group D is placed in the sixth observation chamber 6, the positions of the treatment group C and the treatment group D are changed after 2 times of repeated operation, the operation is repeated for 8 times in total, the positions are changed for 2 times, and the treatment group C and the treatment group D are taken out after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group C and the treatment group D taken out of the S6 into an illumination incubator for culturing for 5 days to respectively obtain a product E and a product F;
s8: counting: and (5) placing the product E and the product F in the S7 under a microscope, and stripping cicada eggs from young stems of tea tips to count the parasitized rate. .
In this embodiment, the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 are all communicated, the fluorescent lamp in S3 is a 40W fluorescent lamp, the temperature and humidity controller in S3 is TDK0302, the sprayer in S4 is DJ-092, the female bee in S1 is a female bee which is allowed to emerge for 24 hours and is obtained by mating groups, and the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 are all made of colorless organic glass and have a length and a width of 20 cm.
It should be noted that the apparatus structure and the drawings of the present invention mainly describe the principle of the present invention, and on the basis of the design principle, the specific connection structure of the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 is not completely described, but on the premise that the person skilled in the art understands the inventive principle, the specific connection structure of the first observation room 1, the second observation room 2, the third observation room 3, the fourth observation room 4, the fifth observation room 5 and the sixth observation room 6 can be clearly known.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. T-shaped observation room with parasitic rate enhanced by volatile takoff compounds, comprising a first observation room (1), a second observation room (2), a third observation room (3), a fourth observation room (4), a fifth observation room (5) and a sixth observation room (6), and is characterized in that the second observation room (2) is positioned between the first observation room (1) and the third observation room (3), the fourth observation room (4) is positioned on one side of the third observation room (3) far away from the second observation room (2), the fifth observation room (5) is positioned on one side of the fourth observation room (4), and the sixth observation room (6) is positioned on one side of the fourth observation room (4) far away from the fifth observation room (5).
2. An observation method for enhancing parasitic rate of parasitic wasps by using a volatile allelochemicals, which is characterized by comprising the following steps:
s1: preparing raw materials: preparing a plurality of clonal potted tea seedlings which are same in tea age and all grow for 3 years and have uniform growth vigor and are not damaged by diseases and insects, performing fertilizer and water management according to the same standard, preparing a plurality of leafhoppers obtained by successive generation propagation in a greenhouse, preparing a plurality of female bees obtained by successive generation propagation by using eggs of the leafhoppers as hosts in the greenhouse, and purchasing allelochemicals and normal hexane from regular suppliers.
S2: spawning: firstly removing leafhoppers and tea seedlings in S1, placing potted tea seedlings in a greenhouse, placing the leafhoppers on the potted tea seedlings to lay eggs for 24 hours, taking 3-5 branches of 1 bud and 4 leaves of tea tips with eggs, taking 50 egg laying holes in each tip as a group, and taking 2 groups;
s3, environment adjustment: the T-shaped observation room is placed in a special laboratory, a fluorescent lamp is arranged right above the T-shaped observation room, light rays are uniformly irradiated from top to bottom, the temperature of the laboratory is controlled to be 24-26 ℃ by a temperature and humidity controller, and the relative humidity is 60-80%.
S4: and (3) concentration adjustment: the taraxacum compound described in S1 was removed and n-hexane was used to prepare a concentration gradient (10)-2g/mL、10-4g/mL、10-6g/mL), filling the solvent A to be detected into a sprayer, and putting n-hexane into the sprayer to obtain a control solvent B;
s5: pretreatment: and uniformly spraying the solvent A to be detected in the S4 on one group of the tips of the tea with the ovum to obtain a treatment group C, and uniformly spraying the contrast solvent B on the other group of the tips of the tea with the ovum to obtain a treatment group D.
S6: releasing bees: 2 female bees in S1 are taken and placed in a second observation chamber (2), 2 female bees in S1 are taken and placed in a fourth observation chamber (4), a treatment group C is placed in a fifth observation chamber (5), a treatment group D is placed in a sixth observation chamber (6), the positions of the treatment group C and the treatment group D are exchanged after 2 times of repeated operation, the total time of repeated operation is 8, the positions are exchanged for 2 times, and the treatment group C and the treatment group D are taken out after the female bees are placed for 12 hours.
S7: culturing: placing the treatment group C and the treatment group D taken out of the S6 into an illumination incubator for culturing for 5 days to respectively obtain a product E and a product F;
s8, statistics: and (5) placing the product E and the product F in the S7 under a microscope, and stripping cicada eggs from young stems of tea tips to count the parasitized rate.
3. The T-shaped observation room for enhancing parasitic rate of parasitic wasps of the volatile taensta compound according to claim 1, characterized in that the first observation room (1), the second observation room (2), the third observation room (3), the fourth observation room (4), the fifth observation room (5) and the sixth observation room (6) are all in communication.
4. The method of claim 2, wherein said S3 fluorescent lamp is a 40W fluorescent lamp.
5. The method of claim 2, wherein the temperature and humidity controller of S3 is model TDK 0302.
6. The method of claim 2, wherein said spray pattern of S4 is DJ-092.
7. The observation method of enhancing the parasitism rate of parasitic wasps by using volatile tassel compounds as claimed in claim 2, wherein the female wasps in S1 are female wasps which are emerged for 24 hours and obtained by mating in groups.
8. The observation method for enhancing the parasitic rate of the parasitic wasps by the volatile taensky compounds according to claim 1, wherein the first observation room (1), the second observation room (2), the third observation room (3), the fourth observation room (4), the fifth observation room (5) and the sixth observation room (6) are made of colorless organic glass and have the length and the width of 20 cm.
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