CN107853058B - Production method of young shoot with tea lesser leafhopper egg - Google Patents
Production method of young shoot with tea lesser leafhopper egg Download PDFInfo
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- CN107853058B CN107853058B CN201711286909.8A CN201711286909A CN107853058B CN 107853058 B CN107853058 B CN 107853058B CN 201711286909 A CN201711286909 A CN 201711286909A CN 107853058 B CN107853058 B CN 107853058B
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- 241001414720 Cicadellidae Species 0.000 title claims abstract description 72
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- 241001122767 Theaceae Species 0.000 title claims abstract 41
- 235000013601 eggs Nutrition 0.000 claims abstract description 67
- 230000001954 sterilising effect Effects 0.000 claims abstract description 9
- 230000001678 irradiating effect Effects 0.000 claims abstract description 5
- 238000005520 cutting process Methods 0.000 claims description 27
- 238000009395 breeding Methods 0.000 claims description 15
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- 239000000243 solution Substances 0.000 claims description 7
- 239000004576 sand Substances 0.000 claims description 6
- 239000011159 matrix material Substances 0.000 claims description 5
- 239000012286 potassium permanganate Substances 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 3
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- 239000007864 aqueous solution Substances 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 claims description 3
- 239000005648 plant growth regulator Substances 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
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- 238000003892 spreading Methods 0.000 claims description 2
- 244000269722 Thea sinensis Species 0.000 description 119
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- 238000012258 culturing Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 201000003872 goiter Diseases 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
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- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a method for producing young shoots with tea lesser leafhopper eggs, which comprises the following steps: step 1: cultivating the tea seedling with the root until the sprout tip of the tea seedling with the root grows to one sprout and four leaves, pulling out the tea seedling with the root, and removing the top bud and the leaves on the sprout tip of the tea seedling with the root; step 2: clamping the root part by using a sponge strip, sterilizing the rooted tea seedling treated in the step (1), transferring the sterilized rooted tea seedling to a soilless seedling tray, putting the whole soilless seedling tray into a container, keeping the sponge strip moist, and putting 7-day-old adult tea lesser leafhopper into the container so that the adult tea lesser leafhopper can lay eggs on the young shoot without bud leaves of the rooted tea seedling; and step 3: and (3) after the adult tea lesser leafhopper in the step (2) is placed in a container for 24 hours, irradiating the bud-free young tips of rooted tea seedlings with white light to drive the adult tea lesser leafhopper, obtaining the young tips with eggs of the tea lesser leafhopper, and storing the young tips in an environment at the temperature of 6.5-7.5 ℃. The invention can produce the young tips with the tea lesser leafhopper eggs, the egg period of which is regular, and the young tips of which are kept fresh for a long time.
Description
Technical Field
The invention relates to the technical field of biological control of insects, in particular to a method for producing young shoots with tea lesser leafhopper eggs.
Background
Tea lesser leafhopper is a main pest in tea garden, mainly relies on chemical control at present, and accounts for 40-60% of the total control cost, so that the problem of drug resistance is prominent, the biological diversity of the tea garden is reduced, the harm is aggravated, and meanwhile, the serious food safety problem is caused.
The small thysanoptera is a key factor for controlling the tea leafhoppers, and artificial propagation and utilization of the small thysanoptera are important means for biological control of the tea leafhoppers. At present, the technology of breeding the small thysanoptera by using artificial feed is not available, so that the breeding of the small thysanoptera by using host tea small green leaf cicada eggs is the only technical approach at present, however, researches show that the small thysanae cannot host the naked eggs of the small tea green leaf cicadas, and for this reason, the production of the young tips with the eggs of the small tea green leaf cicadas becomes an important link for the propagation of the small thysanae population. In vitro tea shoots are easy to oxidize and brown due to polyphenol substances, the preservation time is limited, and the requirements of the breeding process of parasitic wasps such as the egg laying of adult tea lesser leafhoppers, parasitic hatching of gall lesser bees and the like cannot be met; as an improvement, the polyphenol substances in the tea shoot can be prevented from being oxidized by the tree bud tip, so that the preservation time is prolonged, but the biological characteristics and the life habits (tenderness is harmful, and eggs are scattered in bud tip tissues) of the tea lesser leafhopper are utilized in the tree bud tip, so that the egg taking is difficult, and the quality control is difficult.
Meanwhile, the adult tea lesser leafhopper tends to be tender and harmful, can take food on bud leaves and tender stems, is weak and weak, cannot resist hunger, has weak flying force, is more active, can climb and jump, is easy to be frightened and run away, is afraid of wet, intolerant of strong light and direct injection, has the adult period of 25-30 days generally, can copulate and lay eggs after emergence for 1-2 days, has the eggs scattered in bud tip tissues, has the largest number of eggs under the tender tip cortex between two or three leaves under the bud, and can lay 32 eggs per female averagely in spring. It is seen that the physical constitution and behavior of the larvae make it difficult to directly contact the larvae, and the biological characteristics of batch oviposition and long oviposition period make it difficult to obtain the ova with consistent oviposition period, thus being not beneficial to expanding propagation of the larvae population and influencing the biological control in the later period.
Disclosure of Invention
The invention aims to overcome the defects and provide a method for producing young shoots with tea lesser leafhopper eggs, wherein the young shoots are orderly in egg period and can be kept in a fresh and alive state for a long time.
In order to solve the technical problems, the invention adopts the technical scheme that:
a method for producing young shoots with eggs of lesser leafhopper of tea comprises the following steps:
step 1: cultivating tea seedlings with roots in a soilless cuttage mode indoors, pulling out the tea seedlings with roots after the young shoots of the tea seedlings with roots grow to four leaves in one bud, and removing terminal buds and leaves on the young shoots of the tea seedlings with roots;
step 2: sterilizing the rooted tea seedlings treated in the step 1, then transferring the rooted tea seedlings into a soilless seedling tray, fixing root stems of the rooted tea seedlings by using sponge strips and keeping the sponge strips moist, putting the soilless seedling tray with the rooted tea seedlings transferred into a container, and then putting 7-day-old adult tea lesser leafhopper adults into the container so as to enable the adult tea lesser leafhopper adults to lay eggs on bud-free young tips of the rooted tea seedlings;
and step 3: and (3) after the adult tea lesser leafhopper in the step (2) is placed in a container for 24 hours, irradiating the bud-free young tips of the rooted tea seedlings treated in the step (2) by using white light to drive the adult tea lesser leafhopper, obtaining the young tips with eggs of the tea lesser leafhopper, and storing the young tips in an environment at the temperature of 6.5-7.5 ℃.
The invention has the beneficial effects that: (1) according to the method, the tea seedlings with roots are cultured to the young shoots to four leaves with one bud, the top buds and the leaves on the young shoots with the roots are removed, and the young shoots without the buds and the leaves with adventitious roots (the tea seedlings with the roots after being treated in the step 1) are obtained and are used as spawning carriers of the lesser green leafhoppers, and are in a fresh and alive state for a long time relative to the tea tips in vitro, so that the requirements of the lesser green leafhoppers on spawning, lesser goiter parasitic incubation and other parasitic bee breeding processes can be met, the difficulty in egg taking due to the biological characteristics or the life habits of the lesser green leafhoppers can be overcome relative to the tree shoot tips, the young tips with the eggs of the lesser green leafhoppers with the tea eggs can be obtained, and good materials are provided for the artificial propagation of the lesser green leafhopper parasitic bee flies; (2) according to the invention, the same batch of tea lesser leafhopper adults are selected, and the adults in the same container only lay eggs for one day, so that the adults are restrained to lay eggs in time, and the egg period consistency of the obtained eggs can be ensured; (3) the imagoes on the young shoots after spawning are driven away by strong light, the efficiency is high, the bodies of the imagoes are not damaged, the imagoes can be reused, and the imagoes with the eggs of the lesser leafhopper of tea can be produced in batch; (4) the production method is simple to operate, and is suitable for large-scale production of young tips with tea lesser leafhopper eggs in factories for expanding propagation of Thysanoptera ananas populations.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the present invention in detail, the following description is given in detail with reference to the embodiments.
The most key concept of the invention is as follows: the tea seedlings with roots are cultured, the tea seedlings with roots are moved to a soilless seedling tray in a container to keep the roots of the tea seedlings moist, then adult tea lesser leafhopper in the same batch is placed in the container to lay eggs, and the adult tea lesser hopper is repelled to obtain young tips with the eggs of the tea lesser leafhopper, wherein the egg period of the young tips is regular, and the young tips are in a fresh state for a long time.
The production method of the young shoot with the tea lesser leafhopper egg comprises the following steps:
step 1: cultivating tea seedlings with roots in a soilless cuttage mode indoors, pulling out the tea seedlings with roots after the young shoots of the tea seedlings with roots grow to four leaves in one bud, and removing terminal buds and leaves on the young shoots of the tea seedlings with roots;
step 2: sterilizing the rooted tea seedlings treated in the step 1, then transferring the rooted tea seedlings into a soilless seedling tray, fixing root stems of the rooted tea seedlings by using sponge strips and keeping the sponge strips moist, putting the soilless seedling tray with the rooted tea seedlings transferred into a container, and then putting 7-day-old adult tea lesser leafhopper adults into the container so as to enable the adult tea lesser leafhopper adults to lay eggs on bud-free young tips of the rooted tea seedlings;
and step 3: and (3) after the adult tea lesser leafhopper in the step (2) is placed in a container for 24 hours, irradiating the bud-free young tips of the rooted tea seedlings treated in the step (2) by using white light to drive the adult tea lesser leafhopper, obtaining the young tips with eggs of the tea lesser leafhopper, and storing the young tips in an environment at the temperature of 6.5-7.5 ℃.
The sponge strips are adopted to fix the root and stem parts of the root tea seedlings, and the sponge strips are good in moisture absorption and moisture retention, so that the root parts of the root tea seedlings are kept wet all the time, and the tender tips are kept in a fresh and alive state all the time.
From the above description, the beneficial effects of the present invention are: (1) according to the method, the tea seedlings with roots are cultured to the young shoots to four leaves with one bud, the top buds and the leaves on the young shoots with the roots are removed, and the young shoots without the buds and the leaves with adventitious roots (the tea seedlings with the roots after being treated in the step 1) are obtained and are used as spawning carriers of the lesser green leafhoppers, and are in a fresh and alive state for a long time relative to the tea tips in vitro, so that the requirements of the lesser green leafhoppers on spawning, lesser goiter parasitic incubation and other parasitic bee breeding processes can be met, the difficulty in egg taking due to the biological characteristics or the life habits of the lesser green leafhoppers can be overcome relative to the tree shoot tips, the young tips with the eggs of the lesser green leafhoppers with the tea eggs can be obtained, and good materials are provided for the artificial propagation of the lesser green leafhopper parasitic bee flies; (2) according to the invention, the same batch of tea lesser leafhopper adults are selected, and the adults in the same container only lay eggs for one day, so that the adults are restrained to lay eggs in time, and the egg period consistency of the obtained eggs can be ensured; (3) the imagoes on the young shoots after spawning are driven away by strong light, the efficiency is high, the bodies of the imagoes are not damaged, the imagoes can be reused, and the imagoes with the eggs of the lesser leafhopper of tea can be produced in batch; (4) the production method is simple to operate, and is suitable for large-scale production of young tips with tea lesser leafhopper eggs in factories for expanding propagation of Thysanoptera ananas populations.
Further, the specific operation of cultivating the rooted tea seedlings in the soilless cutting mode in the step 1 comprises the following steps:
step a: spreading the sterilized sponge in a sterilized flat-bottom plate, and covering a layer of sterilized fine sand on the sponge to serve as a cuttage matrix;
step b: selecting a small-leaf tea tree variety capable of being subjected to cuttage breeding, cutting short spikes which are stem lignified or semi-lignified, have one complete leaf, are full in axillary buds and are 3-4 cm long from spike strips, cutting the top of each short spike into a flat opening, enabling the distance between the flat opening and a leaf stalk of each short spike to be 0.3-0.5 cm, and cutting the base of each short spike into an inclined plane;
step c: and c, immersing the base part of the short spike treated in the step b into a plant growth regulator aqueous solution for 5-10 s, then cutting the short spike into the cutting medium in the step a, pouring a compound fertilizer solution with the mass concentration of 0.2-0.3% through the cutting medium after cutting, keeping the sponge in a wet state during culture, and controlling the culture temperature to be 27-29 ℃.
According to the description, the cuttage mode enables the cultivated rooted tea seedlings to have high survival rate and good growth vigor, and can provide good carriers for large-scale production of young tips with tea lesser leafhopper eggs.
Further, the thickness of the sponge in the step a is 1-2 cm, and the thickness of the fine sand on the sponge is 1.5-4 cm.
Further, when the cutting is carried out in the step c, the row spacing of the cutting is 6-8 cm, and the minimum distance between adjacent short spikes of the cutting is 2-2.5 cm.
Further, the width of the sponge strips in the step 2 is 2-3 cm.
By the above description, the sponge strip with the width is adopted to clamp the rhizome part of the original root tea seedling, so that the tea seedling is more stable and convenient to use.
Further, in the step 2, the sterilization of the rooted tea seedlings treated in the step 1 is to soak the rooted tea seedlings treated in the step 1 into potassium permanganate solution and then dry the soaked rooted tea seedlings.
Further, the container in the step 2 is a spawning box, the spawning box comprises a box body, a box cover and a box bottom, the box cover and the box bottom are detachably connected with the box body, and the height of the box body of the spawning box is larger than that of a rooted tea seedling with a bud tip with four leaves; the 7-day-old adults of the tea lesser leafhopper are bred in a breeding box matched with the spawning box, wherein the adults of the tea lesser leafhopper are bred by liquid feed in the breeding box. The feeding box also comprises a box body, a top cover and a bottom cover, the top cover and the bottom cover of the feeding box are respectively detachably connected with the box body of the feeding box, and the bottom cover and the box body of the feeding box are detachably connected in a drawing mode.
Further, for further facilitate the use, the upper portion inner wall of the box body of the oviposition box is equipped with the first supporting block that is used for accepting the lid, the box bottom of the oviposition box is equipped with and is used for accepting the second supporting block of box body, after the bottom of raising the box was taken out, the lid of oviposition box was taken off, the box body of raising the box can be placed on first supporting block, and the adult green tea cicada that raises the box can move down naturally. After spawning, when the adults are driven by the LED lamp, the adults climb back into the feeding box, the bottom cover is inserted into the box body, the adults of the tea lesser leafhopper in the feeding box can be used for spawning again every 3 days, and the total frequency does not exceed 9 times.
According to the description, the spawning box is used for placing the bud-free leaf young shoots with adventitious roots, and is matched with a feeding box for use, the feeding environment is separated from the spawning environment, the neatness and the integrity of the two environments are kept, the operation efficiency is high, the production rate is high, and the method is suitable for factory large-scale production; the production process is carried out in a closed space, and the escape of the tea lesser leafhopper adults can be prevented.
Further, the seedling amount of the tea seedlings with roots loaded in each soilless seedling tray in the step 2 can be calculated according to the following formula: the seedling loading of each seedling tray is calculated according to the following formula: y is approximately equal to x multiplied by a multiplied by b divided by c, wherein x is the quantity (head) of the adult green leafhopper tea in the feeding box, a is the egg laying quantity (6 grains/head) per female, b is the proportion (0.5) of the adult female, and c is the target egg laying quantity (20 grains/piece) of each non-bud leaf tip.
Example 1
A method for producing young shoots with eggs of lesser leafhopper of tea comprises the following steps:
step 1: flatly paving a sterilized sponge with the thickness of 1cm in a sterilized flat-bottom plate indoors, and covering a layer of sterilized fine sand with the thickness of 2cm on the sponge to serve as a cuttage matrix (the sponge, the fine sand and the flat-bottom plate are beneficial to high-temperature sterilization or potassium permanganate solution sterilization);
selecting a small-leaf tea tree variety (Fuyun No. 6) capable of being subjected to cuttage propagation, shearing short spikes with complete leaves, strong and full axillary buds and 3-4 cm length from spikes, wherein stems are lignified or semi-lignified, the tops of the short spikes are sheared into flat mouths, the distance between the flat mouths and the leaf stalks of the short spikes is 0.3-0.5 cm, and the bases of the short spikes are sheared into inclined planes;
immersing the base part of the short spike into a plant growth regulator aqueous solution (800 ppm-1000 ppm of naphthylacetic acid NAA) for 5-10 s, then cutting the short spike into the cutting matrix (the row spacing of the cutting is 6cm, the minimum distance between adjacent short spikes of the cutting is 2cm), after cutting, watering the cutting matrix thoroughly by using a compound fertilizer solution with the mass concentration of 0.2-0.3%, keeping the sponge in a wet state during the culture period, controlling the culture temperature at 28 ℃, culturing a rooted tea seedling, pulling out the rooted tea seedling after a new shoot of the rooted tea seedling grows to four leaves of a bud, and removing the terminal bud and the leaves on the new shoot of the rooted tea seedling;
step 2: selecting an oviposition box as a container for placing the rooted tea seedlings processed in the step 1, wherein the oviposition box comprises a box body, a box cover and a box bottom, the box cover and the box bottom are both detachably connected with the box body, and the height of the box body of the oviposition box is greater than that of the rooted tea seedlings with four-leaf buds; the 7-day-old adult tea lesser leafhopper is bred in a breeding box matched with the spawning box, wherein the adult tea lesser leafhopper is bred by liquid feed in the breeding box, a first supporting block for bearing a box cover is arranged on the inner wall of the upper part of a box body of the spawning box, a second supporting block for bearing the box body is arranged at the bottom of the spawning box, after a bottom cover of the breeding box is pulled away and the box cover of the spawning box is taken down, the box body of the breeding box can be placed on the first supporting block, and the adult tea lesser leafhopper in the breeding box can naturally move down;
soaking the rooted tea seedlings treated in the step 1 in a potassium permanganate solution, drying in the shade, sterilizing, transferring to a soilless seedling tray, fixing rootstocks of the rooted tea seedlings by using sponge strips with the width of 2-3 cm, keeping the sponge strips moist, placing the soilless seedling tray with the rooted tea seedlings to an egg laying box (which can be placed at the bottom of the egg laying box first for convenience in placement and then is provided with a box body of the egg laying box), taking a feeding box of adult tea lesser leafhoppers with the age of 7 days, placing a bottom cover of the feeding box on a first receiving block of the egg laying box, and enabling the adult tea lesser leafhoppers to naturally move down to bud-free young tips with the rooted tea seedlings to lay eggs;
and step 3: and (3) after the adult tea lesser leafhopper in the step (2) is placed in a container for 24 hours, irradiating the bud-free young tips of the rooted tea seedlings treated in the step (2) by using white light to drive the adult tea lesser leafhopper, obtaining the young tips with eggs of the tea lesser leafhopper, and storing the young tips in an environment at the temperature of 6.5-7.5 ℃.
Randomly extracting 5 egg shoots, checking the egg laying amount, counting the average value of the egg laying amount, and correcting the seedling loading amount of the seedling tray.
Wherein, the seedling amount of the tea seedlings with roots loaded in each soilless seedling tray in the step 2 can be calculated according to the following formula: the seedling loading of each seedling tray is calculated according to the following formula: y is approximately equal to x multiplied by a multiplied by b divided by c, wherein x is the quantity (head) of the adult green leafhopper tea in the feeding box, a is the egg laying quantity (6 grains/head) per female, b is the proportion (0.5) of the adult female, and c is the target egg laying quantity (20 grains/piece) of each non-bud leaf tip.
In conclusion, according to the method for producing the young tips with the eggs of the tea lesser leafhoppers, provided by the invention, the young tips without the bud leaves (the tea seedlings with the roots after being treated in the step 1) with the adventitious roots are obtained by culturing the tea seedlings with the roots to the young tips with the new buds to the four leaves with the one bud, and the top buds and the leaves on the young tips with the roots are removed, so that the young tips without the bud leaves (the tea seedlings with the roots after being treated in the step 1) are used as spawning carriers of the tea lesser leafhoppers, and are in a fresh and alive state for a long time relative to the tea tips in vitro, so that the requirements of imago spawning, gall lesser wasp parasitic hatching and other parasitic wasp breeding processes of the tea lesser leafhoppers can be met, the difficulties brought by the biological characteristics or the life habits of the tea lesser leafhoppers in egg taking can be overcome relative to the young tips with the quality, and good materials are provided for the artificial propagation of the parasitic wasp with the tea lesser leafhoppers; according to the invention, the same batch of tea lesser leafhopper adults are selected, and the adults in the same container only lay eggs for one day, so that the adults are restrained to lay eggs in time, and the egg period consistency of the obtained eggs can be ensured; the imagoes on the young shoots after spawning are driven away by strong light, the efficiency is high, the bodies of the imagoes are not damaged, the imagoes can be reused, and the imagoes with the eggs of the lesser leafhopper of tea can be produced in batch; the production method is simple to operate, and is suitable for large-scale production of young tips with tea lesser leafhopper eggs in factories for expanding propagation of Thysanoptera ananas populations.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (7)
1. A production method of young shoots with eggs of lesser leafhopper tea is characterized by comprising the following steps:
step 1: cultivating tea seedlings with roots in a soilless cuttage mode indoors, pulling out the tea seedlings with roots after the young shoots of the tea seedlings with roots grow to four leaves in one bud, and removing terminal buds and leaves on the young shoots of the tea seedlings with roots;
step 2: sterilizing the rooted tea seedlings treated in the step 1, then transferring the rooted tea seedlings into a soilless seedling tray, fixing root stems of the rooted tea seedlings by using sponge strips and keeping the sponge strips moist, putting the soilless seedling tray with the rooted tea seedlings transferred into a container, and then putting 7-day-old adult tea lesser leafhopper adults into the container so as to enable the adult tea lesser leafhopper adults to lay eggs on bud-free young tips of the rooted tea seedlings;
and step 3: and (3) after the adult tea lesser leafhopper in the step (2) is placed in a container for 24 hours, irradiating the bud-free young tips of the rooted tea seedlings treated in the step (2) by using white light to drive the adult tea lesser leafhopper, obtaining the young tips with eggs of the tea lesser leafhopper, and storing the young tips in an environment at the temperature of 6.5-7.5 ℃.
2. The method for producing young shoots with eggs of lesser leafhopper of tea as claimed in claim 1, wherein the concrete operation of cultivating rooted tea seedlings by soilless cutting in step 1 comprises the following steps:
step a: spreading the sterilized sponge in a sterilized flat-bottom plate, and covering a layer of sterilized fine sand on the sponge to serve as a cuttage matrix;
step b: selecting a small-leaf tea tree variety capable of being subjected to cuttage breeding, cutting short spikes which are stem lignified or semi-lignified, have one complete leaf, are full of axillary buds and are 3-4 cm long from spike strips, cutting the top of each short spike into a flat opening, enabling the distance between the flat opening and a leaf stalk of each short spike to be 0.3-0.5 cm, and cutting the base of each short spike into an inclined plane;
step c: and c, immersing the base part of the short spike treated in the step b into a plant growth regulator aqueous solution for 5-10 s, then cutting the short spike into the cutting medium in the step a, pouring a compound fertilizer solution with the mass concentration of 0.2-0.3% through the cutting medium after cutting, keeping the sponge in a wet state during culture, and controlling the culture temperature to be 27-29 ℃.
3. The method for producing young shoots with eggs of lesser leafhopper of tea as claimed in claim 2, wherein the thickness of the sponge in step a is 1-2 cm and the thickness of the fine sand on the sponge is 1.5-4 cm.
4. The method for producing young shoots with eggs of lesser leafhopper of tea as claimed in claim 2, wherein the row spacing of the cuttings in step c is 6-8 cm and the spacing between adjacent short shoots of the cuttings is 2-2.5 cm.
5. The method for producing young shoots with eggs of lesser leafhopper of tea as claimed in claim 1, wherein the width of sponge strips in step 2 is 2-3 cm.
6. The method for producing young shoots with eggs of lesser leafhopper of tea as claimed in claim 1, wherein in the step 2, the sterilization of the rooted seedlings treated in the step 1 is carried out by immersing the rooted seedlings treated in the step 1 in potassium permanganate solution and then drying the immersed rooted seedlings.
7. The method for producing young shoots with eggs of lesser leafhopper of tea as claimed in claim 1, wherein the container in step 2 is a spawning box comprising a box body, a box cover and a box bottom, both of which are detachably connected with the box body, the height of the box body of the spawning box is greater than that of rooted tea seedlings with sprouts of one bud and four leaves; the 7-day-old adults of tea lesser leafhopper in the step (a) are raised in a raising box matched with the spawning box.
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| CN115500323A (en) * | 2022-09-15 | 2022-12-23 | 中国农业科学院茶叶研究所 | Method for quickly obtaining large amount of tea lesser leafhopper nymphs |
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| CN103262827B (en) * | 2013-06-01 | 2014-07-09 | 福建农林大学 | Method for feeding tea green leafhoppers indoors |
| CN106719448A (en) * | 2016-12-05 | 2017-05-31 | 福建省农业科学院植物保护研究所 | A kind of method of indoor mass rearing tea lesser leafhopper |
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