CN103858827A - Method for carrying out indoor large-scale diadegma semiclausum hellen breeding - Google Patents
Method for carrying out indoor large-scale diadegma semiclausum hellen breeding Download PDFInfo
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Abstract
The invention discloses a method for carrying out indoor large-scale diadegma semiclausum hellen breeding. The method comprises the steps of (1) cultivation of a host plant of plutella xylostella linnaeus; (2) indoor breeding of the plutella xylostella linnaeus; (3) indoor breeding of the diadegma semiclausum hellen; (4) parasitism of the plutella xylostella linnaeus; (5) feeding of parasitized plutella xylostella linnaeu larvae; (6) disease prevention during the feeding of the parasitized plutella xylostella linnaeu larvae; (7) pupation of the parasitized plutella xylostella linnaeu larvae. According to the method for carrying out the indoor large-scale breeding of the diadegma semiclausum hellen, disclosed by the invention, has the advantages that the method is scientific and reasonable, the operation is simple, convenient and practical, the diadegma semiclausum hellens can be bred in the large scale according to biological characteristics and reproductive behavior requirements of the plutella xylostella linnaeus and the diadegma semiclausum hellen, and thus the field application and the commercialization need of agricultural production can be met.
Description
Technical field
The present invention relates to a kind of indoor propagation method of parasitic enemy insect, specifically the method for the curved tail ichneumon wasp of indoor scale breeding semi-closure.
Background technology
Diamond-back moth
plutella xylostellalinnaeus is subordinate to the Lepidoptera Lepidoptera diamond-back moth Plutellidae of section, is commonly called as " hang spring worm ", " ghost of person who hanged himself ", " pointed at both ends ", main harm crucifer.Diamond-back moth is very strong to adaptive capacity to environment, year occurs from generation to generation many, be generally 8～12 generations in subtropics, the torrid zone is that 20 generations are above, generation overlap phenomenon serious, reproduction rate and occurrence frequency high, harm is serious, difficulty of prevention and cure is large, and agricultural chemicals is had the advantages that to resistance development is very fast, resistant multiple is higher, become the important pests of brassicaceous vegetable, brassicaceous vegetable is caused to catastrophic collapse, it is estimated, the whole world reaches 1,000,000,000 dollars for preventing and treating the expense of diamond-back moth and causing damage every year.Difficulty, the drug-fast generation of control and use in a large number the vicious circle of chemical insecticide, has brought a series of economy, society and ecological problem.
The curved tail ichneumon wasp of semi-closure
diadegma semiclausumhellen is under the jurisdiction of Insecta (Insecta), Hymenoptera (Hymenoptera), Ichneumonoidea (Ichneumonoidea) Ichneumonidae (Ichneumonidae), it is the advantage parasitic enemy insect of diamond-back moth, the larva of parasitic diamond-back moth, solitary parasitism honeybee.
With originating from Europe, there is harm in whole Europe to diamond-back moth and play continuous and effective control action in the curved tail ichneumon wasp of semi-closure and diamond-back moth.A series of society, ecology and the economic problems brought for solving diamond-back moth chemical pesticide control, people have carried out correlative study to the curved tail ichneumon wasp of semi-closure, and be all to study qualitatively a bit mostly, raising scale is also less, do not carry out indoor scale breeding, be difficult to meet field application and commercialization breeding.
Summary of the invention
The object of the present invention is to provide one can realize the curved tail ichneumon wasp of biological control semi-closure, apply and make the method for the curved tail ichneumon wasp of its commercial a kind of indoor scale breeding semi-closure to meet the field of agricultural production.
Technical scheme of the present invention: the method for the curved tail ichneumon wasp of a kind of indoor scale breeding semi-closure, concrete steps are as follows:
(1) cultivation of diamond-back moth host plant: host plant is selected wild cabbage, sows cabbage seed in seedling-cultivating tray, grow to 5～6 leaf ages and transplant to planting alms bowl after emerging, when wild cabbage grows to 10～12 effective blade, for subsequent use;
(2) indoor propagation of diamond-back moth: the built-in case of laying eggs of propagation house, in within 3-8 days, laying eggs case, put into 300-800 pickles chrysalis, pupa provides supplements after turning into adult, the hydromel that supplements is is 15% containing honey weight ratio, and in supplements, add streptomycin, then put into the paper tinsel of laying eggs and collect Eggs of Diamondback Moth, collect every day and the replacing paper tinsel of laying eggs; After being carried out disinfection, receives by the ovum paper tinsel formalin of collecting on the wild cabbage in plantation alms bowl, the wild cabbage that is connected to Eggs of Diamondback Moth is connected to alms bowl, and to be placed on temperature be 25 ± 2 ℃, humidity is 65%, light application time is to grow in the propagation house of 14:10, provides parasitic for breeding the curved tail ichneumon wasp of semi-closure in the time that diamondback moth larvae is grown to 3 age;
(3) the curved tail ichneumon wasp of semi-closure indoor propagation: the built-in numerous beehive of propagation house, every 3-6 days putting into the curved tail ichneumon wasp of semi-closure bee pupa in numerous beehive, throwing in density is 180-220 head/m3, pupa provides supplements after turning into adult, supplements is the hydromel of honey density 15%, and in supplements, to add weight ratio be 0.2% streptomycin;
(4) diamond-back moth parasitism: by cultivation have 3 age diamondback moth larvae complete stool wild cabbage put into numerous beehive, allow the naturally parasitic diamondback moth larvae of the curved tail ichneumon wasp of semi-closure adult, the ratio of the curved tail ichneumon wasp of semi-closure adult quantity and diamondback moth larvae quantity is 1:40～50; In parasitic processes, on the base plate of numerous beehive, the cabbage leaves that place mat is fresh; Regularly to raising bee colony rejuvenation in numerous beehive;
(5) raised by parasitic diamondback moth larvae: after 3 days, take out by the parasitic diamondback moth larvae of crossing from numerous beehive, being placed on temperature is 22 ± 2 ℃, and humidity is 60-70%, on the culturing rack in the propagation house that light application time is 14:8, raises, and food is wild cabbage;
(6) by the disease prevention and cure in parasitic diamondback moth larvae raising: with aqueous solution of chlorine dioxide, receptacle was regularly carried out to spraying disinfection every 1 month;
(7) pupated by parasitic diamondback moth larvae: after being pupated by parasitic diamondback moth larvae, collect in time bee pupa, continue the curved tail ichneumon wasp of breeding semi-closure as semi-closure curved tail ichneumon wasp product and the curved tail ichneumon wasp of semi-closure kind bee pupa.
The described rejuvenation of step (4) is: while breeding in numerous beehive, add the 30-50% honeybee kind under the natural conditions of field to carry out rejuvenation at quarterly intervals in the bee colony in numerous beehive.
Step (4) described in parasitic processes, the fresh cabbage leaves of place mat below Brussels sprouts, in the plantation alms bowl of described Brussels sprouts below and on the base plate of numerous beehive, for the diamondback moth larvae falling down from wild cabbage strain provides food, and there is the cabbage leaves of diamondback moth larvae to be placed on pot culture wild cabbage by being scattered in time.
What step (6) was described regularly carried out spraying disinfection with 0.05% aqueous solution of chlorine dioxide to receptacle every 1 month.
Invention main points of the present invention are that 3 processes of host plant cultivation, host egg and breeding natural enemy that the curved tail ichneumon wasp of breeding semi-closure is needed organically combine, to reach the object of the curved tail ichneumon wasp of biological control semi-closure.Biological control and the production process of the curved tail ichneumon wasp of semi-closure are: be first to select suitable plant wild cabbage; wild cabbage is as the host plant of diamond-back moth; first it is edible to be that diamond-back moth is liked, and is also easy to cultivation, and the standardized planting of wild cabbage is the important step that guarantees the curved tail ichneumon wasp of biological control semi-closure.Second step is biological control host diamond-back moth, finally reaches the object of the curved tail ichneumon wasp of biological control semi-closure.The parasitism focusing on diamondback moth larvae of the present invention.In numerous beehive, to put into the curved tail ichneumon wasp of semi-closure bee pupa every 3-6 days for guaranteeing in numerous beehive that have the one-tenth honeybee of posting the curved tail ichneumon wasp of blodynamic semi-closure, the present invention every day.Find after deliberation, in diamond-back moth parasitic processes, not the more multiparasitism rate of one-tenth honeybee of the curved tail ichneumon wasp of semi-closure is higher, diamondback moth larvae is in certain numerous beehive space, the curved tail ichneumon wasp of semi-closure adult has each other disturbance reponse in parasitic processes, after adult exceedes some, its disturbance reponse will be stronger, make it find effect and find diamond-back moth parasitic probability attenuating, thereby affect its parasitic rate.Think that after deliberation it is 180-220 head/m3 that the curved tail ichneumon wasp of semi-closure adult is thrown in density, in table 1.Through evidence, the curved tail ichneumon wasp of semi-closure bee pupa turns into after adult in time to Adult supplement nutrient, can significantly improve Adult worms producting eggs amount and life-span, improves reproduction rate.Test shows to provide 15% hydromel, and nutrition is the most suitable as a supplement, and in the extra-nutrition providing, adds 0.2% streptomycin can significantly improve by the survival rate of parasitic larva, in table 2, thereby has improved the reproductive efficiency of the curved tail ichneumon wasp of semi-closure.In parasitic processes, because the parasitic quantity of the curved tail ichneumon wasp of semi-closure adult is limited, and its parasitic ability is also limited, in order to give full play to the parasitic ability of adult, access in numerous beehive 3 age diamondback moth larvae quantity should become according to the curved tail ichneumon wasp of semi-closure in numerous beehive the quantity of honeybee, and in conjunction with diamondback moth larvae quantity, the curved tail ichneumon wasp of semi-closure is found to effect and decides.Our research show that the curved tail ichneumon wasp of semi-closure adult quantity and diamondback moth larvae quantity optimal proportion are that 1:40～50 are for best, in table 3.In parasitic processes, because diamondback moth larvae is subject to stimulation and the scaring of the curved tail ichneumon wasp of semi-closure adult parasitizing behavior, approximately 80% is sagging by parasitic larva suspension, be scattered on the plantation soil surface of alms bowl and the base plate of numerous beehive, therefore timely some fresh cabbage leaves of place mat on plantation alms bowl soil surface and numerous beehive base plate, the diamondback moth larvae that assurance is fallen down has fresh wild cabbage can supply to take food, and the cabbage leaves that has in time a large amount of diamondback moth larvaes by being scattered is placed on potted plant wild cabbage and completes growth to guarantee that larva can be transferred on fresh vegetable leaf, this part parasitized larvae rate reaches 78.43%, table 4.After diamondback moth larvae was by parasitic 3 days, its taking-up is placed on and on culturing rack, takes food growth, in adult parasitism and larvae development process, provide applicable environmental condition can guarantee ovum and larva normal development and Fast Growth, particularly at specific temperature, parasitic rear its offspring's female-male proportion can be controlled at 1:1, and such female-male proportion is conducive to indoor continuous breeding most.Parasitic and grow under such condition, development duration and the eclosion rate of the curved tail ichneumon wasp of semi-closure are the most applicable, and female-male proportion can be controlled at 1:1.04, for the indoor scale of the curved tail ichneumon wasp of semi-closure is produced assurance is provided continuously; Due to parasitic relative airtight with the receptacle of growing the curved tail ichneumon wasp of semi-closure, thereby easily infected mortality of larva, for preventing that larva from infecting the survival rate that guarantees larva, with the aqueous solution of chlorine dioxide containing 0.05%, receptacle is regularly carried out to spraying disinfection every other month, eliminate bacterium and fungi in receptacle, can effectively reduce infected rate and the lethality of larva, thereby improve by the survival rate of parasitic larva, in table 5, and then improve the reproductive efficiency of the curved tail ichneumon wasp of semi-closure.The curved tail ichneumon wasp of semi-closure connects the degeneration that can cause indoor population for raising for a long time indoor, the fertility of its population and vitality can reduce, to arrive at quarterly intervals a collection of honeybee kind under field acquisition natural conditions, mix and raise collecting honeybee kind under natural conditions and indoor honeybee kind, indoor honeybee kind is carried out to rejuvenation, thus the fertility of population and vitality raising in holding chamber.Reasonable, the easy and simple to handle practicality of methodological science of the present invention, can, by the biological property of diamond-back moth and the curved tail ichneumon wasp of semi-closure and the curved tail ichneumon wasp of reproductive behavior requirement biological control semi-closure, apply and make its commercialization needs to meet the field of agricultural production.
specific implementation method
one, working environment:
1, plastic tunnel: for the large-scale planting plantation of wild cabbage, when in plastic tunnel when temperature drift, be unfavorable for growing of wild cabbage, adopt the cooling of fan drencher system.
2, diamond-back moth propagation house: surrounding has wall, has roof and door and window.For host egg diamond-back moth, according to the growth requirement of diamond-back moth, diamond-back moth propagation house temperature is controlled at 25 ± 2 ℃.
3, the curved tail ichneumon wasp breeding of semi-closure propagation house: for breeding the curved tail ichneumon wasp of semi-closure, laboratory temperature is controlled at 22 ± 2 ℃.
4, numerous beehive: size is the stainless steel frame of 120cm × 60cm × 180cm, centre cuts off and is divided into 3 with plank, and every increases illumination system, the back side and side glass capsulation, twice sliding doors is done in front, seals by gauze clamshell doors, carries out the operation in reproductive process by door.
5, culturing rack: size is the angle iron frame of 160cm × 125cm × 60cm, middle plank cuts off and is divided into 3 layers, and every floor height 50cm, installs two of 40 watts of fluorescent lamps on every layer by totally three layers.
6, Oviposition of Diamondback Moth case: size is the wood frame of 25cm × 30cm × 25cm, top glass capsulation, so that observe, He Liangge side, the back side seals with 80 order gauzes, so that ventilated, bottom is plank, sliding door is done in front one, carries out the operation in experimentation by switch gate.
7, Oviposition of Diamondback Moth paper tinsel: select household fresh-keeping masking foil to make the ovum chaff of 2cm × 10cm, with ready wild cabbage immersion bubble ovum paper tinsel, ovum paper tinsel dries concentrated for subsequent use after soaking.
8, hydromel: the hydromel of compound concentration 15% is for the extra-nutrition of diamond-back moth adult and the curved tail ichneumon wasp of semi-closure adult;
9, head cabbage varieties: host plant is selected wild cabbage, kind is the rich series in capital.
Two,
the standardized planting of wild cabbage
The selection of wild cabbage and seeding and seedling raising: wild cabbage, kind is the rich series in capital, gets seedling-cultivating tray, 2 cabbage seeds of every bunch planting, after planting note water and fertilizer management and temperature control.
Cabbage seedling is transplanted: sow after 30 days when cabbage seedling grows to 5～6 leaf age, choose healthy and strong cabbage seedling and transplant to plastics plantation alms bowl, transplant and after 40 days, work as cabbage seedling and grow to 10～12 effective blades, the at this moment best leaf age strain phase for providing diamondback moth larvae to take food.
Rich water and other management after transplanting: after transplanting, need to water in time normal root water, after transplant survival, rich water is watered in timing, find that there is when other insects are caused harm as aphid or cabbage caterpillar and will prevent and treat in time, within first 15 days, can not use any agricultural chemicals wild cabbage inoculation diamond-back moth.
three, cabbage moth scale propagation technique:
The collection of Eggs of Diamondback Moth: put into 300-800 pickles chrysalis in laying eggs case in 3-8 days, when pickles chrysalis turns into after adult, 15% the hydromel extra-nutrition as adult is provided, in case, hang 4～5 and make for subsequent use paper tinsel of laying eggs for diamond-back moth Adult worms producting eggs laying eggs, collect every day and change the paper tinsel of laying eggs of laying eggs in case.
The hatching of Eggs of Diamondback Moth: get the Eggs of Diamondback Moth of collecting, put it in formalin thimerosal and soak 15 minutes, then use distilled water rinsing 3 times, remove the thimerosal on ovum paper tinsel, dry, be placed on and get on wild cabbage for subsequent use, the ovum amount that connects of every basin wild cabbage is 400.
The breeding of diamondback moth larvae: wild cabbage connects after Eggs of Diamondback Moth, places it in T:25 ± 2 ℃ under indoor conditions, RH:60-70%, and light application time: L:D=14:10 allows its hatching.After 2 days, ovum hatching is larva, and 5 days grow is afterwards 3 instar larvaes, and the curved tail ichneumon wasp of semi-closure adult parasitism is provided.
four, the curved tail ichneumon wasp of semi-closure indoor scale propagation technique
The maintenance of the curved tail ichneumon wasp of semi-closure adult quantity and supplementing in numerous beehive: throwing in density was 180-220 head/m putting into the curved tail ichneumon wasp of semi-closure bee pupa in numerous beehive in 3-8 days
3in numerous beehive of present embodiment, placed 100 the curved tail ichneumon wasp of semi-closure pupas every 3-8 days and sprout wings by it, bee pupa turns into adult, and the extra-nutrition of 15% hydromel as adult is provided, note changing every day hydromel, guarantee that the one-tenth honeybee quantity of the curved tail ichneumon wasp of semi-closure in numerous beehive every day is at 30-50 couple.
The parasitism of the curved tail ichneumon wasp of semi-closure to diamondback moth larvae: having in numerous beehive of the curved tail ichneumon wasp of semi-closure adult, the diamondback moth larvae to 3 ages is grown in access, become the quantity of honeybee according to the curved tail ichneumon wasp of semi-closure in numerous beehive, becoming the ratio of honeybee quantity and diamondback moth larvae quantity according to the curved tail ichneumon wasp of semi-closure is 1:40～50, access diamondback moth larvae quantity.In parasitic processes, to pad fresh cabbage leaves at numerous bottom board and plantation alms bowl surface, exchange the cabbage leaves of pad in bottom every day for, collect the larva dropping in parasitic processes.The parasitic conditions of adult is T:22 ± 2 ℃, RH:60-70%, and light application time: L:D=14:8-12, after parasitic 3 days is placed on its taking-up and on culturing rack, takes food growth.
Growth management after parasitism: take out from numerous beehive by the parasitic diamondback moth larvae of crossing, being placed on culturing rack allows it take food growth, developmental condition is T:22 ± 2 ℃, RH:65%, light application time: L:D=14:10, the situation of growing and the Brussels sprouts of observing larva are taken food situation, and guarantee the daily management of watering of Brussels sprouts, avoid Brussels sprouts lack of water here to wither and affect the normal growth of diamondback moth larvae, in the time that Brussels sprouts leaf is taken food totally, the blade of cutting diamondback moth larvae is placed directly on fresh Brussels sprouts, pupates until grow.
The collection of the curved tail ichneumon wasp of semi-closure bee pupa: the development progress that takes food of observing larva on culturing rack every day, after parasitic 7 days, larvae development is pupa, collect the curved tail ichneumon wasp of semi-closure pupa, the curved tail ichneumon wasp of semi-closure pupa is canescence, the blunt circle in two ends, be cylinder shape, as semi-closure curved tail ichneumon wasp product and the curved tail ichneumon wasp of semi-closure kind bee pupa.
Due to parasitic relative airtight with the receptacle of growing the curved tail ichneumon wasp of semi-closure, thereby easily infected mortality of larva of long duration, with 0.05% chlorine dioxide, receptacle is regularly carried out to spraying disinfection every other month, eliminate bacterium and fungi in receptacle, reduce infected rate and the lethality of larva, thereby the Fast Growth that guarantees larva is grown, for the raising of the curved tail ichneumon wasp of semi-closure is given security; In the indoor continuous feeding process of the curved tail ichneumon wasp of semi-closure, owing to connecting for a long time the degeneration that can cause indoor population for raising indoor, the fertility of its population and vitality can reduce, to arrive at quarterly intervals a collection of honeybee kind under field acquisition natural conditions, mix and raise collecting honeybee kind 20-30% under natural conditions and indoor honeybee kind, indoor honeybee kind is carried out to rejuvenation, thus the fertility of population and vitality raising in holding chamber.
The different parasitic rates of throwing in the curved tail ichneumon wasp of semi-closure under density of table 1
Table 2 adds the impact of streptomycin on diamondback moth larvae survival rate in extra-nutrition
The parasitic rate of the curved tail ichneumon wasp of semi-closure under the different diamond-back moth quantity of table 3
Table 4 from the diamondback moth larvae that falls down of wild cabbage of plantation by parasitic rate
| Diamondback moth larvae quantity (head) | 150 | 185 | 176 | 206 | 193 | 188 | 175 | 214 | 208 | 195 |
| The curved tail ichneumon wasp of semi-closure bee pupa quantity (head) | 113 | 142 | 140 | 167 | 155 | 143 | 130 | 175 | 170 | 151 |
| Parasitic rate (%) | 75.33 | 76.76 | 79.55 | 81.07 | 80.31 | 76.06 | 74.29 | 81.78 | 81.73 | 77.44 |
Table 5 utilizes the impact of disinfection by chlorine dioxide on diamondback moth larvae survival rate
Claims (4)
1. a method for the curved tail ichneumon wasp of indoor scale breeding semi-closure, is characterized in that, concrete steps are as follows:
(1) cultivation of diamond-back moth host plant: host plant is selected wild cabbage, sows cabbage seed in seedling-cultivating tray, grow to 5～6 leaf ages and transplant to planting alms bowl after emerging, when wild cabbage grows to 10～12 effective blade, for subsequent use;
(2) indoor propagation of diamond-back moth: the built-in case of laying eggs of propagation house, in within 3-8 days, laying eggs case, put into 300-800 pickles chrysalis, pupa provides supplements after turning into adult, the hydromel that supplements is is 15% containing honey weight ratio, and in supplements, add streptomycin, then put into the paper tinsel of laying eggs and collect Eggs of Diamondback Moth, collect every day and the replacing paper tinsel of laying eggs; After being carried out disinfection, receives by the ovum paper tinsel formalin of collecting on the wild cabbage in plantation alms bowl, the wild cabbage that is connected to Eggs of Diamondback Moth is connected to alms bowl, and to be placed on temperature be 25 ± 2 ℃, humidity is 65%, light application time is to grow in the propagation house of 14:10, provides parasitic for breeding the curved tail ichneumon wasp of semi-closure in the time that diamondback moth larvae is grown to 3 age;
(3) the curved tail ichneumon wasp of semi-closure indoor propagation: the built-in numerous beehive of propagation house, every 3-6 days putting into the curved tail ichneumon wasp of semi-closure bee pupa in numerous beehive, throwing in density is 180-220 head/m3, pupa provides supplements after turning into adult, supplements is the hydromel of honey density 15%, and in supplements, to add weight ratio be 0.2% streptomycin;
(4) diamond-back moth parasitism: by cultivation have 3 age diamondback moth larvae complete stool wild cabbage put into numerous beehive, allow the naturally parasitic diamondback moth larvae of the curved tail ichneumon wasp of semi-closure adult, the ratio of the curved tail ichneumon wasp of semi-closure adult quantity and diamondback moth larvae quantity is 1:40～50; In parasitic processes, on the base plate of numerous beehive, the cabbage leaves that place mat is fresh; Regularly to raising bee colony rejuvenation in numerous beehive;
(5) raised by parasitic diamondback moth larvae: after 3 days, take out by the parasitic diamondback moth larvae of crossing from numerous beehive, being placed on temperature is 22 ± 2 ℃, and humidity is 60-70%, on the culturing rack in the propagation house that light application time is 14:8, raises, and food is wild cabbage;
(6) by the disease prevention and cure in parasitic diamondback moth larvae raising: with aqueous solution of chlorine dioxide, receptacle was regularly carried out to spraying disinfection every 1 month;
(7) pupated by parasitic diamondback moth larvae: after being pupated by parasitic diamondback moth larvae, collect in time bee pupa, continue the curved tail ichneumon wasp of breeding semi-closure as semi-closure curved tail ichneumon wasp product and the curved tail ichneumon wasp of semi-closure kind bee pupa.
2. the method for the curved tail ichneumon wasp of a kind of indoor Fast-propagation semi-closure as claimed in claim 1, it is characterized in that, the described rejuvenation of step (4) is: while breeding in numerous beehive, add the 30-50% honeybee kind under the natural conditions of field to carry out rejuvenation at quarterly intervals in the bee colony in numerous beehive.
3. the method for the curved tail ichneumon wasp of a kind of indoor Fast-propagation semi-closure as claimed in claim 1, it is characterized in that, step (4) described in parasitic processes, the fresh cabbage leaves of place mat below Brussels sprouts, in the plantation alms bowl of described Brussels sprouts below and on the base plate of numerous beehive, for the diamondback moth larvae falling down from wild cabbage strain provides food, and there is the cabbage leaves of diamondback moth larvae to be placed on pot culture wild cabbage by being scattered in time.
4. the method for the curved tail ichneumon wasp of a kind of indoor scale breeding semi-closure as claimed in claim 1, is characterized in that, what step (6) was described regularly carried out spraying disinfection with 0.05% aqueous solution of chlorine dioxide to receptacle every 1 month.
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| CN109197786B (en) * | 2018-10-31 | 2021-10-29 | 山西农业大学 | Method for breeding large amount of semi-closed curvicauda curvularia indoors |
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| CN114176047A (en) * | 2021-12-16 | 2022-03-15 | 重庆市农业科学院 | Breeding method of long-distance ichneumon fly of looper buzus tungensis |
| CN117136913A (en) * | 2023-08-31 | 2023-12-01 | 浙江大学 | Indoor propagation method for grassland caterpillar wasp |
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