CN104304183A - Artificial propagation and production method for diadromus collaris - Google Patents
Artificial propagation and production method for diadromus collaris Download PDFInfo
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- CN104304183A CN104304183A CN201410427556.9A CN201410427556A CN104304183A CN 104304183 A CN104304183 A CN 104304183A CN 201410427556 A CN201410427556 A CN 201410427556A CN 104304183 A CN104304183 A CN 104304183A
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- diadromus
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- back moth
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- 241000256806 Diadromus collaris Species 0.000 title claims abstract description 87
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 20
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 102
- 241000256844 Apis mellifera Species 0.000 claims abstract description 60
- 241000196324 Embryophyta Species 0.000 claims description 49
- 241000382353 Pupa Species 0.000 claims description 39
- 230000024241 parasitism Effects 0.000 claims description 32
- 230000003071 parasitic effect Effects 0.000 claims description 27
- 238000011081 inoculation Methods 0.000 claims description 20
- 235000021110 pickles Nutrition 0.000 claims description 20
- 230000017448 oviposition Effects 0.000 claims description 15
- 229920003023 plastic Polymers 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 5
- 235000006008 Brassica napus var napus Nutrition 0.000 claims description 4
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 4
- 241001674939 Caulanthus Species 0.000 claims description 4
- 230000032669 eclosion Effects 0.000 claims description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 210000004681 ovum Anatomy 0.000 claims description 3
- 230000029264 phototaxis Effects 0.000 claims description 3
- 238000009825 accumulation Methods 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 9
- 235000013311 vegetables Nutrition 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 238000010924 continuous production Methods 0.000 abstract description 2
- 230000001902 propagating effect Effects 0.000 abstract 2
- 238000009341 apiculture Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 241001608896 Cotesia vestalis Species 0.000 description 8
- 230000005540 biological transmission Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000012364 cultivation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 241001454993 Cotesia rubecula Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000500441 Plutellidae Species 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004920 integrated pest control Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of biological control of agricultural pests and aims at providing an artificial propagation and production method for diadromus collaris. The method comprises the following steps: collecting and propagating bee species, producing hosts, inoculating and propagating plutella xylostella, developing plutella xylostella pupae, inoculating the diadromus collaris, culturing and collecting the diadromus collaris, and reserving the diadromus collaris species. According to the method, the superior bee species selection, bee propagation and beekeeping technology is perfected and the diadromus collaris is subjected to high-quality efficient large-batch propagation; in addition, the large-scale continuous production can be performed, the method is applied in the field of agricultural pest control and used for preventing the plutella xylostella, the agricultural production needs are met to a great extent, and very convenience is provided for biological control of cruciferous vegetable pests.
Description
Technical field
The invention relates to pest field of biological control, particularly Diadromus collaris artificial propagation production method.
Background technology
Diamond-back moth Plutella xylostella (Linnaeus) belongs to Lepidoptera diamond-back moth section, rise in Mediterranean Region the earliest, the brassicaceous vegetable such as main harm rape, wild cabbage, Chinese cabbage, radish, host reaches kind more than 40, is the primary pest of worldwide brassicaceous vegetable.The adaptive capacity to environment of diamond-back moth is very strong, the fifties in last century, just there are some researches show that it creates very high resistance to many chemical pesticides.To its control, oneself becomes a difficult problem for puzzlement many countries and regions in the world.Therefore, concentrate on more and more and utilize the hostile diamond-back moth in sky to control in integrated pest control management.
Parasitic wasp is the Important Natural Enemy resource of diamond-back moth, and utilizing parasitic wasp to carry out biological control is an effective way.Notification number is that the patent of invention of CN103503836A discloses plutella xylostella cotesia rubecula artificial propagation production method, achieve high-quality, efficiently, in enormous quantities breed Cotesia plutellae larvae, can produce on a large scale, be applied to the biological control of pest, for preventing and treating the primary pest diamond-back moth on brassicaceous vegetable.
But Cotesia plutellae larvae is parasitic diamondback moth larvae only, particularly two, third-instar larvae.By when field adaptability Cotesia plutellae larvae prevents and treats diamond-back moth, can not ensure that diamond-back moth is all in larval phase, the diamond-back moth having entered pupa time can not be parasitic by Cotesia plutellae larvae; And the parasitic rate of Cotesia plutellae larvae is also by the impact of the factor such as temp. and humidity, weather, can normally not pupated by diamondback moth larvae on parasitism, becomes the worm source of continuing harm brassicaceous vegetable.
Diadromus collaris Diadromus collaris (Gravenhorst) is the main parasitic honeybee in diamond-back moth pupa time, extensively distributes in European, African and Asia.The successful example of this honeybee of existing introduction control diamond-back moths such as Australia, India, the U.S. and relevant report.This honeybee all has distribution on Beijing of China, Shanxi, Zhejiang, Henan, Ningxia and Inner Mongol and other places, and Diadromus collaris diamond-back moth in parasitic pupa time also completes growth, has higher parasitic rate, be suitable for biological control and field large-area applications in field.
Field discharges Diadromus collaris and Cotesia plutellae larvae simultaneously, the parasitic diamondback moth larvae of Cotesia plutellae larvae, and is in pupa time and diamond-back moth that is parasitic by Cotesia plutellae larvae and that continue to pupate can by Diadromus collaris parasitism.Honeybee number of times is put in such field of reducing, and reduce cost accounting, effectively preventing diamond-back moth, avoids the harm again of diamond-back moth.But the domestic research report that there is not yet Diadromus collaris biological control and large area Field information and control efficiency aspect.
Summary of the invention
Main purpose of the present invention is to overcome deficiency of the prior art, provides a kind of Diadromus collaris artificial propagation production method can laid a good foundation for China biological control diamond-back moth.For solving the problems of the technologies described above, solution of the present invention is:
Diadromus collaris artificial propagation production method is provided, comprises the steps:
(1) gather and breed honeybee kind;
(2) host is produced;
(3) inoculation reproduction of diamond-back moth;
(4) growth of pickles chrysalis;
(5) Diadromus collaris inoculation;
(6) Diadromus collaris is cultivated and is collected;
(7) reserve seed for planting;
Described step (1) specifically comprises:
A, field bee kind: (occurring on serious field plant blade diamond-back moth) gathers by the pupa of Diadromus collaris parasitism;
B, honeybee kind are purified and preferably: steps A is collected by the pupa of Diadromus collaris parasitism, be placed in that temperature is 23 ~ 25 DEG C, RH grows under the condition of 50 ~ 70%, sprout wings after honeybee, be transferred in diamond-back moth body in pupa time; Then screen, the queen bee of selective body length between 4.5 ~ 5.0 millimeters and the Xiong Feng of body length between 5.0 ~ 5.5 millimeters are as the honeybee kind of artificial propagation;
C, honeybee kind expand numerous: the honeybee kind selected in step B is carried out single head parasitism and expand numerous, the queen bee and the diamond-back moth at single pupa initial stage that are about to mating are placed in dactylethrae, after queen bee parasitism is laid eggs, shift out by the pickles chrysalis of parasitism, and move into new pickles chrysalis; Be cultured to sprout wings by the pickles chrysalis of parasitism honeybee to what obtain, until accumulation 100 female peaks of Diadromus collaris Xiong Feng and 500 Diadromus collaris;
D, honeybee kind are preserved: will grow to the Diadromus collaris in first pupa time in step C, are stored into that temperature is 10 ± 1 DEG C, RH preserves under the condition of 45 ~ 55%;
Described step (2) specifically comprises: choose host plant, and (conveniently cultivation method) is sowed, transplant seedlings and after field planting host plant, be in (cleaning) glass greenhouse between 22 ~ 28 DEG C in temperature, cultivates host plant; When host plant seedling grows to 6,7 true leaves, Plant colonization is in flowerpot, and a collection of host plant of field planting week about, often criticize 50 strain host plant seedlings; The host plant seedling growing to 9,10 true leaves after getting field planting is for subsequent use;
Described step (3) specifically comprises:
The preparation in E, diamond-back moth worm source: in the previous moon carrying out step F, the host plant seedling for subsequent use got in 15 basin steps (2) is put into diamond-back moth receptacle and (is purchased from Zhejiang Qiushi Artificial Environment Co., Ltd., Zhejiang University's Electrical utility firms) in, and inoculate diamond-back moth adult 2500 ~ 4500, for subsequent use when filial generation diamond-back moth adult eclosion;
F, the filial generation diamond-back moth adult sprouting wings in step e are transferred in Oviposition of Diamondback Moth gathering unit, get hydromel to feed filial generation diamond-back moth adult, oopod collection is carried out when each oopod there being 500 ~ 1000 ovum, get the host plant seedling for subsequent use in step (2), the oopod of collection to be laid on host plant seedling and the oopod more renewed in Oviposition of Diamondback Moth gathering unit;
G, get the host plant seedling being covered with oopod in 35 basin step F, move into diamondback moth larvae and cultivate and grow room (being purchased from Zhejiang Qiushi Artificial Environment Co., Ltd., Zhejiang University's Electrical utility firms), cultivate diamond-back moth and grow;
When H, diamond-back moth in step G pupate in a large number, pick out and be no less than the Oviposition of Diamondback Moth gathering unit that 500 pupas are placed in step F and sprout wings, to ensure the quantity of diamond-back moth adult in Oviposition of Diamondback Moth gathering unit;
Described step (4) specifically comprises: treat the diamondback moth larvae hatching in step G on host plant seedling, host plant seedling is moved into pickles chrysalis cultivation growth room and (be purchased from Zhejiang Qiushi Artificial Environment Co., Ltd., Zhejiang University's Electrical utility firms), get grow for subsequent use to the diamond-back moth in pupa time;
Described step (5) specifically comprises:
I, to start front ten days that carry out step J, the honeybee kind in step D is being placed in temperature is 23 ~ 25 DEG C, RH is being that to be developed to adult under the condition of 50 ~ 70% for subsequent use;
The pickles chrysalis 1000 of 1 ~ 3 age in days of body green in J, selecting step (4), be moved in parasitic tank, and move into Diadromus collaris adult parasitism inoculation in step I, after 48 hours, utilize Diadromus collaris to become the phototaxis principle of honeybee, worm pupa and honeybee are separated;
Described step (6) specifically comprises: when in step (5) by the diamond-back moth pupa development of parasitism by the 7th day, diamond-back moth pupal cell is substantially totally digested, Diadromus collaris starts to cocoon when pupating (pupa is grey), (conventional method) gathers, packs or refrigerates for subsequent use to Diadromus collaris cocoon, for dispensing or direct Field information;
Described step (7) specifically comprises: Diadromus collaris pupa 500 conditions according to step D of getting in step (6) preserve honeybee kind, for Diadromus collaris inoculation during next breeding.
In the present invention, when carrying out parasitism inoculation in described step (5), ensure that Diadromus collaris is the Diadromus collaris being in emergence period, diamond-back moth is the diamond-back moth being in pupa time.
In the present invention, described host plant adopts wild cabbage.
In the present invention, when carrying out parasitism inoculation in described step B, the number ratio of Diadromus collaris and pickles chrysalis is between 1:5 ~ 8.
In the present invention, when carrying out parasitism inoculation in described step B, the male and female number ratio of Diadromus collaris is between 4 ~ 6:1.
In the present invention, in described step (6), Diadromus collaris cocoon carry out providing and delivering or Field information time, the male and female number ratio of Diadromus collaris cocoon is between 4 ~ 6:1; During Field information, Diadromus collaris cocoon and diamondback moth larvae parasitic wasp discharge simultaneously.
In the present invention, the parasitic tank in described step J adopts basal diameter to be 25cm, the high cylindrical transparent plastic bottle for 20cm.
In the present invention, Oviposition of Diamondback Moth gathering unit utilizes host plant blade to collect the device of laying eggs of diamond-back moth, comprises cuboid framework, two gauzes, splash guard, three blocks of light transmission plastic plates, door, vial, freshness protection package, culture dish, light sources.Two gauzes are arranged on the relative side of two of cuboid framework respectively, splash guard is arranged on the bottom surface of cuboid framework, with end face, one piece of light transmission plastic plate is respectively installed before cuboid framework, below, on the light transmission plastic plate that door is arranged on before cuboid framework, light source be arranged on below light transmission plastic plate on.Culture dish and vial are placed in cuboid lower portion respectively, and freshness protection package for after wrapping up host plant blade, is inserted in (filling water) vial.
Compared with prior art, the invention has the beneficial effects as follows:
The advantage honeybee kind that adopted technical scheme provided by the invention perfect is selected, numerous honeybee and put bee technology, realizes high-quality, efficiently, in enormous quantities breeds Diadromus collaris;
Production method of the present invention can carry out large-scale continuous production, is applied to agricultural insect pests control field, for preventing and treating diamond-back moth insect, thus meets agricultural production needs to a greater extent, for the biological control of Cruciferous Vegetable Pests provides convenience;
Production method of the present invention has done special control to the cultivation of Diadromus collaris, storage, thus the reasonable inoculation of more convenient parasitic wasp and utilization;
The inoculative proportion of production method of the present invention to Diadromus collaris, diamond-back moth has done requirement, concentrates in parasitic tank when inoculation is parasitic by host, greatly shortens the time of Diadromus collaris host-location, thus improves the parasitic rate of parasitic wasp;
In production seeded process of the present invention, make the emergence period of Diadromus collaris as far as possible and match the pupa time of diamond-back moth, for parasitic wasp parasitism, laying eggs provides more multimachine meeting, thus improves egg laying amount and parasitic rate;
Diadromus collaris of the present invention is the natural advantage natural enemy of diamond-back moth, has good Natural control action to diamond-back moth;
Production method of the present invention has reference role to the breeding of other honeybee, storage and release;
The present invention is that good basis has been established in the breeding utilization of China diamond-back moth parasitic wasp.
Accompanying drawing explanation
Fig. 1 is Diadromus collaris artificial progagation schematic flow sheet of the present invention.
Fig. 2 is the Oviposition of Diamondback Moth gathering unit overall structure front view in the present invention.
Reference numeral in figure is: 1 cuboid framework; 2 gauzes; 3 splash guards; 4 light transmission plastic plates; 5; 6 vials; 7 host plant blades; 8 freshness protection packages; 9 culture dishes; 10 light sources.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the present invention is described in further detail:
As shown in Figure 1, Diadromus collaris artificial propagation production method comprises the steps:
(1) gather and breed honeybee kind;
(2) host is produced;
(3) inoculation reproduction of diamond-back moth;
(4) growth of pickles chrysalis;
(5) Diadromus collaris inoculation;
(6) Diadromus collaris is cultivated and is collected;
(7) reserve seed for planting.
Described step (1) specifically comprises:
A, field bee kind: occur serious field plant blade gathers by the pupa of Diadromus collaris parasitism diamond-back moth;
B, honeybee kind are purified and preferably: steps A is collected by the pupa of Diadromus collaris parasitism, be placed in that temperature is 23 ~ 25 DEG C, RH grows under the condition of 50 ~ 70%, sprout wings after honeybee, be transferred in diamond-back moth body in pupa time, the honeybee newly gone out is screened, selective body length in the queen bee of 4.5-5.0 millimeter and body length at the Xiong Feng of 5.0-5.5 millimeter as the honeybee kind of artificial propagation.
C, honeybee kind expand numerous: the honeybee kind selected in step B is carried out single head parasitism and expand numerous, the queen bee and the diamond-back moth at single pupa initial stage that are about to mating are placed in dactylethrae, after queen bee parasitism is laid eggs, shift out by the pickles chrysalis of parasitism, and move into new pickles chrysalis; Cultivate parasitic rear pickles chrysalis and honeybee to sprouting wings; Accumulate the female peak of Diadromus collaris of about 100 about Diadromus collaris Xiong Feng and 500;
D, honeybee kind are preserved: will grow to the Diadromus collaris in first pupa time in step C, are stored into that temperature is 10 ± 1 DEG C, RH preserves under the condition of 45 ~ 55%.
Described step (2) specifically comprises: choose wild cabbage as host plant, and conveniently cultivation method is sowed, transplanted seedlings and after field planting host plant, in clean glass greenhouse, cultivate host plant; When host plant seedling grows to 6,7 true leaves, Plant colonization is in flowerpot, and a collection of host plant of field planting week about, often criticize host plant seedling 50 strain; The host plant seedling growing to 9,10 true leaves after getting field planting is for subsequent use.
Described step (3) specifically comprises:
E, carrying out step (3) the previous moon, host plant seedling for subsequent use after last field planting in 15 basin steps (2) is put into diamond-back moth receptacle and (is purchased from Zhejiang Qiushi Artificial Environment Co., Ltd., Zhejiang University's Electrical utility firms) in, and inoculate diamond-back moth adult 2500 ~ 4500, for subsequent use when filial generation diamond-back moth adult eclosion; ;
F, the filial generation diamond-back moth adult sprouting wings in step e are transferred in Oviposition of Diamondback Moth gathering unit, get hydromel to feed filial generation diamond-back moth adult, when each oopod there being 500 ~ 1000 ovum, the oopod that the host plant seedling that collection oopod is for subsequent use after being laid in the last field planting in step (2) also more renews;
G, get be covered with in 35 basin F chalaza host plant seedling move into diamondback moth larvae cultivate grow room (being purchased from Zhejiang Qiushi Artificial Environment Co., Ltd., Zhejiang University's Electrical utility firms), cultivate diamond-back moth grow;
When H, diamond-back moth in step G pupate in a large number, pick out and be no less than the Oviposition of Diamondback Moth gathering unit that 500 pupas are placed in step F and sprout wings, to ensure the quantity of diamond-back moth adult in Oviposition of Diamondback Moth gathering unit;
Described step (4) specifically comprises: treat the diamondback moth larvae hatching in step G on host plant seedling, host plant seedling is moved into pickles chrysalis cultivation growth room and (be purchased from Zhejiang Qiushi Artificial Environment Co., Ltd., Zhejiang University's Electrical utility firms), get grow for subsequent use to the diamond-back moth in pupa time;
Described step (5) specifically comprises:
A, start to carry out step (5) front ten days, the honeybee kind in step (1) D is placed in temperature is 23 ~ 25 DEG C, RH is that to be developed to adult under the condition of 50 ~ 70% for subsequent use;
The pickles chrysalis about 1000 of the 1-3 age in days of body green in B, selecting step (4), be moved in parasitic tank, move into Diadromus collaris adult parasitism inoculation in steps A, after 48 hours simultaneously, utilize Diadromus collaris to become the phototaxis principle of honeybee, worm pupa and honeybee are separated;
Described step (6) specifically comprises: when in step (5) by the diamond-back moth pupa development of parasitism by the 7th day, diamond-back moth pupal cell is substantially totally digested, Diadromus collaris starts to cocoon when pupating (pupa is grey), conventional method gathers, packs or refrigerates for subsequent use to Diadromus collaris cocoon, and should collect in time, store, for dispensing or direct Field information.Should suitably moisturizing during storage, and period of storage is unsuitable oversize, along with the prolongation of storage period, the eclosion rate of Diadromus collaris constantly can reduce along with the prolongation of storage period; Generally be advisable within 15 days.Diadromus collaris cocoon carry out providing and delivering or Field information time, the male and female number ratio of Diadromus collaris cocoon is between 4 ~ 6:1; During Field information, Diadromus collaris cocoon and diamondback moth larvae parasitic wasp discharge simultaneously.
Described step (7) specifically comprises: Diadromus collaris pupa about 500 conditions according to step (1) D of getting in step (6) preserve honeybee kind, for Diadromus collaris inoculation during next breeding.
As shown in Figure 2, Oviposition of Diamondback Moth gathering unit comprises cuboid framework 1, two gauzes 2, splash guard 3, three blocks of light transmission plastic plates 4, door 5, vial 6, freshness protection package 8, culture dish 9, light sources 10.
Two gauzes 2 are arranged on the relative side of two of cuboid framework 1 respectively, splash guard 3 is arranged on the bottom surface of cuboid framework 1, respectively install one piece of light transmission plastic plate 4 with end face before cuboid framework 1, below, door 5 is arranged on the light transmission plastic plate 4 before cuboid framework 1; It is inner that culture dish 9 and vial 6 are placed in cuboid framework 1 respectively, and freshness protection package 8, for after wrapping up host plant blade 7, is inserted in and fills in the vial 6 of water.
Finally, it should be noted that above what enumerate is only specific embodiments of the invention.Obviously, the invention is not restricted to above embodiment, a lot of distortion can also be had.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (7)
1. Diadromus collaris artificial propagation production method, is characterized in that, comprises the steps:
(1) gather and breed honeybee kind;
(2) host is produced;
(3) inoculation reproduction of diamond-back moth;
(4) growth of pickles chrysalis;
(5) Diadromus collaris inoculation;
(6) Diadromus collaris is cultivated and is collected;
(7) reserve seed for planting;
Described step (1) specifically comprises:
A, field bee kind: gather by the pupa of Diadromus collaris parasitism;
B, honeybee kind are purified and preferably: steps A is collected by the pupa of Diadromus collaris parasitism, be placed in that temperature is 23 ~ 25 DEG C, RH grows under the condition of 50 ~ 70%, sprout wings after honeybee, be transferred in diamond-back moth body in pupa time; Then screen, the queen bee of selective body length between 4.5 ~ 5.0 millimeters and the Xiong Feng of body length between 5.0 ~ 5.5 millimeters are as the honeybee kind of artificial propagation;
C, honeybee kind expand numerous: the honeybee kind selected in step B is carried out single head parasitism and expand numerous, the queen bee and the diamond-back moth at single pupa initial stage that are about to mating are placed in dactylethrae, after queen bee parasitism is laid eggs, shift out by the pickles chrysalis of parasitism, and move into new pickles chrysalis; Be cultured to sprout wings by the pickles chrysalis of parasitism honeybee to what obtain, until accumulation 100 female peaks of Diadromus collaris Xiong Feng and 500 Diadromus collaris;
D, honeybee kind are preserved: will grow to the Diadromus collaris in first pupa time in step C, are stored into that temperature is 10 ± 1 DEG C, RH preserves under the condition of 45 ~ 55%;
Described step (2) specifically comprises: choose host plant, sow, transplant seedlings and after field planting host plant, be in the glass greenhouse between 22 ~ 28 DEG C in temperature, cultivates host plant; When host plant seedling grows to 6,7 true leaves, Plant colonization is in flowerpot, and a collection of host plant of field planting week about, often criticize 50 strain host plant seedlings; The host plant seedling growing to 9,10 true leaves after getting field planting is for subsequent use;
Described step (3) specifically comprises:
The preparation in E, diamond-back moth worm source: in the previous moon carrying out step F, the host plant seedling for subsequent use got in 15 basin steps (2) puts into diamond-back moth receptacle, and inoculate diamond-back moth adult 2500 ~ 4500, for subsequent use when filial generation diamond-back moth adult eclosion;
F, the filial generation diamond-back moth adult sprouting wings in step e are transferred in Oviposition of Diamondback Moth gathering unit, get hydromel to feed filial generation diamond-back moth adult, oopod collection is carried out when each oopod there being 500 ~ 1000 ovum, get the host plant seedling for subsequent use in step (2), the oopod of collection to be laid on host plant seedling and the oopod more renewed in Oviposition of Diamondback Moth gathering unit;
G, get the host plant seedling being covered with oopod in 35 basin step F, move into diamondback moth larvae and cultivate and grow room, cultivate diamond-back moth and grow;
When H, diamond-back moth in step G pupate in a large number, pick out and be no less than the Oviposition of Diamondback Moth gathering unit that 500 pupas are placed in step F and sprout wings, to ensure the quantity of diamond-back moth adult in Oviposition of Diamondback Moth gathering unit;
Described step (4) specifically comprises: treat the diamondback moth larvae hatching in step G on host plant seedling, host plant seedling is moved into pickles chrysalis and cultivates and grow room, get grow for subsequent use to the diamond-back moth in pupa time;
Described step (5) specifically comprises:
I, to start front ten days that carry out step J, the honeybee kind in step D is being placed in temperature is 23 ~ 25 DEG C, RH is being that to be developed to adult under the condition of 50 ~ 70% for subsequent use;
The pickles chrysalis 1000 of 1 ~ 3 age in days of body green in J, selecting step (4), be moved in parasitic tank, and move into Diadromus collaris adult parasitism inoculation in step I, after 48 hours, utilize Diadromus collaris to become the phototaxis principle of honeybee, worm pupa and honeybee are separated;
Described step (6) specifically comprises: when in step (5) by the diamond-back moth pupa development of parasitism by the 7th day, diamond-back moth pupal cell is substantially totally digested, Diadromus collaris starts to cocoon when pupating, gather, pack or refrigerate for subsequent use to Diadromus collaris cocoon, for dispensing or direct Field information;
Described step (7) specifically comprises: Diadromus collaris pupa 500 conditions according to step D of getting in step (6) preserve honeybee kind, for Diadromus collaris inoculation during next breeding.
2. Diadromus collaris artificial propagation production method according to claim 1, it is characterized in that, when carrying out parasitism inoculation in described step (5), ensure that Diadromus collaris is the Diadromus collaris being in emergence period, diamond-back moth is the diamond-back moth being in pupa time.
3. Diadromus collaris artificial propagation production method according to claim 1, is characterized in that, described host plant adopts wild cabbage.
4. Diadromus collaris artificial propagation production method according to claim 1, is characterized in that, when carrying out parasitism inoculation in described step B, the number ratio of Diadromus collaris and pickles chrysalis is between 1:5 ~ 8.
5. Diadromus collaris artificial propagation production method according to claim 1, is characterized in that, when carrying out parasitism inoculation in described step B, the male and female number ratio of Diadromus collaris is between 4 ~ 6:1.
6. Diadromus collaris artificial propagation production method according to claim 1, is characterized in that, in described step (6), Diadromus collaris cocoon carry out providing and delivering or Field information time, the male and female number ratio of Diadromus collaris cocoon is between 4 ~ 6:1; During Field information, Diadromus collaris cocoon and diamondback moth larvae parasitic wasp discharge simultaneously.
7. Diadromus collaris artificial propagation production method according to claim 1, is characterized in that, the parasitic tank in described step J adopts basal diameter to be 25cm, the high cylindrical transparent plastic bottle for 20cm.
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