CN107996523A - The method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp - Google Patents
The method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp Download PDFInfo
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- CN107996523A CN107996523A CN201711274100.3A CN201711274100A CN107996523A CN 107996523 A CN107996523 A CN 107996523A CN 201711274100 A CN201711274100 A CN 201711274100A CN 107996523 A CN107996523 A CN 107996523A
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- 238000009395 breeding Methods 0.000 title claims abstract description 28
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 235000013311 vegetables Nutrition 0.000 title claims abstract description 24
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 23
- 241000255777 Lepidoptera Species 0.000 title claims abstract description 20
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 43
- 235000011293 Brassica napus Nutrition 0.000 claims abstract description 18
- 240000008100 Brassica rapa Species 0.000 claims abstract description 18
- 235000000540 Brassica rapa subsp rapa Nutrition 0.000 claims abstract description 18
- 230000024241 parasitism Effects 0.000 claims abstract description 13
- 241000500441 Plutellidae Species 0.000 claims abstract description 12
- 230000008635 plant growth Effects 0.000 claims abstract description 10
- 239000000725 suspension Substances 0.000 claims abstract description 10
- 230000012447 hatching Effects 0.000 claims abstract description 7
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- 239000011521 glass Substances 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 241001608896 Cotesia vestalis Species 0.000 claims description 7
- 230000013011 mating Effects 0.000 claims description 5
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- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 4
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- 240000007124 Brassica oleracea Species 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Feed For Specific Animals (AREA)
- Catching Or Destruction (AREA)
Abstract
The present invention relates to a kind of method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp, comprise the following steps：（1）Field gathers parasitic wasp bee cocoon；（2）Obtained parasitic wasp bee cocoon to be collected is identified spare after hatching；（3）Planting pot plantation Turnip Sprouts are utilized under the conditions of plant growth light source；（4）Diamondback moth is bred indoors using suspension method；（5）Step（2）In obtained parasitic wasp mate；（6）Selecting step（4）In obtained 2 ~ 3 age diamondback moths be placed in parasitic cup, and by step（5）Obtained parasitic wasp introduces parasitic cup and carries out parasitism；（7）The parasitic wasp bee cocoon obtained in parasitic cup is collected, clears up parasitic cup；（8）Repeat the above steps（2）~（7）, continue generation breeding diamondback moth and parasitic wasp.The present invention is easy to operate, efficiently convenient, of low cost, can breed Cruciferae lepidoptera pest parasitic wasp by rapid amplifying indoors, have broad application prospects.
Description
Technical field
The present invention relates to a kind of method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp.
Background technology
Diamondback moth is the important pests of brassicaceous vegetable, it has generation, the wherein Yangtze river basin and east in each province of China
Southern coastal area occurs more serious.Diamondback moth category oligophagy pest, main harm wild cabbage, leaf mustard, Chinese cabbage, cauliflower, cabbage heart etc.
Brassicaceous vegetable.In recent years, the Cruciferae vegetable cultivated area of global range is continuously increased, early in 2009, just
Up to 3,400,000 hectares of cultivated area.However, with the increase of brassicaceous vegetable cultivated area, the harm of diamondback moth is also more
Seriously, it loses caused by being endangered in the whole world reaches annual hundred million dollars of 40-50.In the developing country such as China peasant in order to prevent
Diamondback moth excessively uses chemical pesticide, as a result causes diamondback moth that various pesticides are produced rapidly with resistance, and the prevention of diamondback moth is even more
As a difficult problem of the pendulum in face of the production of China brassicaceous vegetable.Moreover, further the recognizing to " 3R " problem with people
Know, people control diamondback moth also more and more urgent for seeking method more environmentally friendly beyond chemical prevention.
At present, the prevention to diamondback moth mainly has the comprehensive processing methods such as biological control, chemical prevention, cultural control, changes
Learn the primary means that the use that prevention is insecticide is still diamondback moth prevention.The major measure of cultural control is Cruciferae and non-
Work and crop rotation etc. between brassicaceous vegetable；In terms of biological control, the biology available for prevention diamondback moth mainly has parasitics
Insect, pathogenetic bacteria, disease fungus, virus, microsporidian and nematode.In parasitic insect, parasitic wasp research is more, but by
In natural enemy insect and pest outburst there are following effect, often when diamondback moth breaks out greatly, the quantity of parasitic wasp also not up to needs
The level asked, therefore the successful case successfully using natural enemy prevention diamondback moth is actually rare.
Cotesia plutellae larvae is the internal excited state of diamondback moth larvae phase, is distributed from south to Beijing in China, is small
The sociales insect parasite of diamond-back moth.Existing Cotesia plutellae larvae reproduction technique is the Cotesia plutellae larvae by field collection through sieve
The host plant raising diamondback moth for growing to 5-8 piece true leaves is recycled after choosing, the time-consuming occupation of land of plantation of host plant, is difficult to realize dish
The rapid amplifying of moth disk braconid wasp.
The content of the invention
In view of the deficiencies in the prior art, the technical problems to be solved by the invention are to provide a kind of indoor rapid amplifying breeding
The method of brassicaceous vegetable lepidoptera pest parasitic wasp, it is not only reasonable in design but also efficiently convenient.
In order to solve the above-mentioned technical problem, the technical scheme is that：A kind of interior rapid amplifying breeding Cruciferae
The method of vegetables lepidoptera pest parasitic wasp, comprises the following steps：
（1）Field gathers parasitic wasp bee cocoon；
（2）Obtained parasitic wasp bee cocoon to be collected is identified spare after hatching；
（3）Planting pot plantation Turnip Sprouts are utilized under the conditions of plant growth light source；
（4）Diamondback moth is bred indoors using suspension method；
（5）Step（2）In obtained parasitic wasp mate；
（6）Selecting step（4）In obtained 2 ~ 3 age diamondback moths be placed in parasitic cup, and by step（5）Obtained parasitic wasp introduces
Parasitic cup carries out parasitic；
（7）The parasitic wasp bee cocoon obtained in parasitic cup is collected, clears up parasitic cup；
（8）Repeat the above steps（2）~（7）, continue generation breeding diamondback moth and parasitic wasp.
Preferably, in step（1）In, when field gathers parasitic wasp bee cocoon, collected in the blade back of crucifer parasitic
Bee bee cocoon, finger-type glass tube is positioned over by single parasitic wasp bee cocoon, and at least one drop honey is stained with the pipe of finger-type glass tube, is referred to
The mouth of pipe of type glass tube clogs sealing with cotton, is identified after parasitic wasp bee cocoon is hatched spare.
Preferably, the parasitic wasp uses Cotesia plutellae larvae.
Preferably, in step（3）In, plant growth light source is plant growth LED light or fluorescent lamp.
Preferably, in step（3）In, plant nutrient soil or organic soil are sprinkling upon in planting pot, plant nutrient is native or has
The bottom of machine matter soil mulching plant basin, and sprinkle radish seed after drenching plant nutrient soil or organic soil with water and planted.
Preferably, in step（4）In, the suspension method refers to：Diamondback moth pupa and adult is placed in blake bottle, the training
The one side for supporting bottle is provided with through hole, and the blake bottle is provided with a suspension down of through hole, and the lower section of the blake bottle is placed
Turnip Sprouts, Oviposition of Diamondback Moth in the bottle of blake bottle, the diamondback moth larvae of hatching dropped by through hole carried out on Turnip Sprouts feeding,
Growth.
Preferably, in step（5）In, when parasitic wasp mates, female, drone pairing is put in observation in mating cage and mates or puts
When the cage 24 that mates is small and acquiescence has mated.
Preferably, the parasitic cup includes cup body, and the opening of the cup body is provided with cup lid, is set in the middle part of the cup lid
Venthole is equipped with, gauze is provided with the venthole, one section of adhesive tape is pasted in the cup lid, is stained with what at least one drop was hung upside down on adhesive tape
Honey and an at least wet cotton, honey supply parasitic wasp feeding, and wet cotton is for parasitic wasp supplement moisture content.
Preferably, step（9）, after parasitic wasp raising reaches 10 ~ 100, collect bee cocoon and repeat step above-mentioned steps
（2）~（5）, selecting step（4）In obtained 2 ~ 3 age diamondback moths be placed in parasitic cage, and by step（5）Obtained parasitic wasp is drawn
Enter parasitic cage and carry out parasitism, being transferred to other parasitic cages by parasitic 2-3 age diamondback moths is raised, and collects bee cocoon, cleaning is posted
Raw cage, and repeat step above-mentioned steps（2）~（5）、（9）, continue generation breeding diamondback moth and parasitic wasp, until parasitic wasp raising reaches
1000.
Preferably, one section of adhesive tape is pasted in the parasitic cage, the projecting honey of at least one drop is stained with adhesive tape and at least one wet
Cotton, honey supply parasitic wasp feeding, and wet cotton is for parasitic wasp supplement moisture content.
Compared with prior art, the invention has the advantages that：The present invention is easy to operate, and efficiently convenient, cost is low
It is honest and clean, Cruciferae lepidoptera pest parasitic wasp can be bred by rapid amplifying indoors, had broad application prospects.
The present invention will be further described in detail with reference to the accompanying drawings and detailed description.
Brief description of the drawings
Fig. 1 is the organigram of the embodiment of the present invention.
Fig. 2 is the organigram of the embodiment of the present invention.
In figure：1- parasitism cups, 101- cup bodies, 102- cup lids, 103- ventholes, 2- parasitism cages, 201- slide fasteners, 3- honey,
The wet cottons of 4-.
Embodiment
To make the foregoing features and advantages of the present invention clearer and more comprehensible, special embodiment below, and coordinate attached drawing, make detailed
Carefully it is described as follows.
As shown in Figure 1 and 2, a kind of method of indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp, bag
Include following steps：
（1）Field gathers parasitic wasp bee cocoon；
（2）Obtained parasitic wasp bee cocoon to be collected is identified spare after hatching；
（3）Planting pot plantation Turnip Sprouts are utilized under the conditions of plant growth light source；
（4）Diamondback moth is bred indoors using suspension method；
（5）Step（2）In obtained parasitic wasp mate；
（6）Selecting step（4）In obtained 2 ~ 3 age diamondback moths be placed in parasitic cup 1, and by step（5）Obtained parasitic wasp is drawn
Enter parasitic cup 1 and carry out parasitism；
（7）The parasitic wasp bee cocoon obtained in parasitic cup 1 is collected, clears up parasitic cup 1；
（8）Repeat the above steps（2）~（7）, continue generation breeding diamondback moth and parasitic wasp.
In embodiments of the present invention, in step（1）In, when field gathers parasitic wasp bee cocoon, in the leaf of crucifer
The back of the body collects parasitic wasp bee cocoon, single parasitic wasp bee cocoon is positioned over finger-type glass tube, and be stained with least in the pipe of finger-type glass tube
One drop honey, the mouth of pipe of finger-type glass tube clog sealing with cotton, and spare, identification parasitic wasp is identified after parasitic wasp bee cocoon is hatched
Whether bee cocoon is Cotesia plutellae larvae bee cocoon.
In embodiments of the present invention, the parasitic wasp uses Cotesia plutellae larvae.
In embodiments of the present invention, in step（3）In, plant growth light source is plant growth LED light or fluorescent lamp.
In embodiments of the present invention, in step（3）In, plant nutrient soil or organic soil are sprinkling upon in planting pot, plant
The bottom of Nutrition Soil or organic soil mulching plant basin, and sprinkle radish seed after drenching plant nutrient soil or organic soil with water
Planted.
In embodiments of the present invention, in step（4）In, the suspension method refers to：Diamondback moth pupa and adult is placed in culture
In bottle, the one side of the blake bottle is provided with through hole, and the blake bottle is provided with a suspension down of through hole, the blake bottle
Lower section place Turnip Sprouts, in the bottle of blake bottle, the diamondback moth larvae of hatching is dropped in Turnip Sprouts Oviposition of Diamondback Moth by through hole
Upper progress feeding, growth.
In embodiments of the present invention, in step（5）In, when parasitic wasp mates, female, drone pairing is put in mating cage and seen
Examine mating or be placed in mating cage 24 it is small when and give tacit consent to mated.
In embodiments of the present invention, the parasitic cup 1 includes cup body 101, and the opening of the parasitism cup 1 is provided with cup lid
102, the middle part of the cup lid 102 is provided with venthole 103, is provided with gauze at the venthole 103, in the cup lid 102
One section of adhesive tape is pasted, the projecting honey of at least one drop is stained with adhesive tape and an at least wet cotton, honey supply parasitic wasp feeding, wet cotton
Moisture content is supplemented for parasitic wasp.
In embodiments of the present invention, step（9）, after parasitic wasp raising reaches 10 ~ 100, collect bee cocoon and repeat to walk
Rapid above-mentioned steps（2）~（5）, selecting step（4）In obtained 2-3 age diamondback moths be placed in parasitic cage 2, and by step（5）Obtain
Parasitic wasp introduce parasitic cage 2 and carry out parasitism, being transferred to other parasitic cages 2 by parasitic 2-3 age diamondback moths is raised, and is collected
Bee cocoon, clears up parasitic cage 2, and repeat step above-mentioned steps（2）~（5）、（9）, continue generation breeding diamondback moth and parasitic wasp, until posting
Raw bee raising reaches 1000.
In embodiments of the present invention, one section of adhesive tape of patch in the parasitic cage 2, be stained with adhesive tape the projecting honey of at least one drop with
An at least wet cotton, honey supply parasitic wasp feeding, and wet cotton is for parasitic wasp supplement moisture content.
In embodiments of the present invention,
（1）The parasitic wasp worm sources of beginning can be seldom with quantity, as long as having a queen bee, a drone to carry out parasitic honeybee kind
The amplification breeding of group；
（2）Requirement to space is small, as long as indoor an insectary or greenhouse, you can carry out the plantation of Turnip Sprouts, the feeding of diamondback moth
Foster and the raw bee of bee breeding；
（3）The rapid amplifying of parasitic wasp quantity is realized, when starting worm sources are a pair of female drone, it is only necessary to be within 2 weeks amplifiable to arrive 80-
200 bees；In the case where diamondback moth larvae quantity is enough, parasitic wasp quantity can be expanded to more than 1000 in one month；
（4）It is of the invention not to be subject to seasonal restrictions, it annual can uninterruptedly provide diamond-back moth disk suede cocoon queen bee and be used for diamondback moth control or section
Learn research；
（5）Indoor parasitic wasp population quantity can be regulated and controled as needed, only need to be carried out with parasitic cup 1 when requirement is little
Conservation continues generation breeding, and the method that parasitic 2 endoparasitism of cage raising can be timely used when demand is big expands parasitic wasp population quantity.
In embodiments of the present invention, prepare to have sowed the Turnip Sprouts of one week first, using ten Turnip Sprouts as one plant of wet cotton of use
Wrap up its root and with one layer of masking foil outsourcing, a pair of of parasitic wasp that the same day sprouts wings is put into parasitic cup, the bottom of parasitic cup is put
Enter a piece of qualitative filter paper, while be put into above-mentioned Turnip Sprouts and 50 first 2 ~ 3 age diamondback moths, be stained with a drop honey on the inside of cup lid, and covering
Inside open an osculum and fill in wet cotton and supply parasitic wasp feeding；Parasitic wasp is transferred to another parasitism for having diamondback moth and Turnip Sprouts after one day
Cup, repeats the above steps until queen bee is dead, the diamondback moth after parasitism is raised in parasitic cup；Cocoon 112, plumage are received after 7 ~ 10 days
Change to obtain queen bee 36, drone 46.
In embodiments of the present invention, drone 30 is taken, queen bee 30, is put into parasitic cage, and a disk Turnip Sprouts are put in parasitic cage,
The Turnip Sprouts containing diamondback moth are transferred to new parasitic cage after having 2 ~ 3 age diamondback moth 500 ~ 800, one day in Turnip Sprouts, are being posted
The parasitic cage of raw bee reapposes the Turnip Sprouts containing 500 ~ 800 diamondback moths, repeats the above steps once；Cocoon 663 is received after 7 ~ 10 days
A, sprout wings to obtain parasitic wasp 502.
The present invention is not limited to above-mentioned preferred forms, anyone can show that other are each under the enlightenment of the present invention
The method of the indoor rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp of kind form.It is all according to the present patent application patent
The equivalent changes and modifications that scope is done, should all belong to the covering scope of the present invention.
Claims (10)
- A kind of 1. method of interior rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp, it is characterised in that including Following steps：（1）Field gathers parasitic wasp bee cocoon；（2）Obtained parasitic wasp bee cocoon to be collected is identified spare after hatching；（3）Planting pot plantation Turnip Sprouts are utilized under the conditions of plant growth light source；（4）Diamondback moth is bred indoors using suspension method；（5）Step（2）In obtained parasitic wasp mate；（6）Selecting step（4）In obtained 2 ~ 3 age diamondback moths be placed in parasitic cup, and by step（5）Obtained parasitic wasp introduces Parasitic cup carries out parasitic；（7）The parasitic wasp bee cocoon obtained in parasitic cup is collected, clears up parasitic cup；（8）Repeat the above steps（2）~（7）, continue generation breeding diamondback moth and parasitic wasp.
- 2. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：In step（1）In, when field gathers parasitic wasp bee cocoon, parasitic wasp bee is collected in the blade back of crucifer Cocoon, finger-type glass tube is positioned over by single parasitic wasp bee cocoon, and at least one drop honey, finger-type glass are stained with the pipe of finger-type glass tube The mouth of pipe of glass pipe clogs sealing with cotton, is identified after parasitic wasp bee cocoon is hatched spare.
- 3. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：The parasitic wasp uses Cotesia plutellae larvae.
- 4. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：In step（3）In, plant growth light source is plant growth LED light or fluorescent lamp.
- 5. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：In step（3）In, plant nutrient soil or organic soil are sprinkling upon in planting pot, plant nutrient soil or organic matter The bottom of native mulching plant basin, and sprinkle radish seed after drenching plant nutrient soil or organic soil with water and planted.
- 6. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：In step（4）In, the suspension method refers to：Diamondback moth pupa and adult is placed in blake bottle, the blake bottle One side be provided with through hole, the blake bottle is provided with a suspension down of through hole, and radish is placed in the lower section of the blake bottle Seedling, for Oviposition of Diamondback Moth in the bottle of blake bottle, the diamondback moth larvae of hatching is dropped by through hole carries out feeding, life on Turnip Sprouts It is long.
- 7. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：In step（5）In, when parasitic wasp mates, female, drone pairing is put in observation in mating cage and mates or be placed in friendship With cage 24 it is small when and give tacit consent to mated.
- 8. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：The parasitism cup includes cup body, and the opening of the cup body is provided with cup lid, is provided with the middle part of the cup lid Venthole, is provided with gauze at the venthole, one section of adhesive tape of patch in the cup lid, is stained with the projecting honey of at least one drop on adhesive tape With an at least wet cotton, honey supplies parasitic wasp feeding, and wet cotton is for parasitic wasp supplement moisture content.
- 9. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 1, It is characterized in that：Step（9）, after parasitic wasp raising reaches 10 ~ 100, collect bee cocoon and repeat step above-mentioned steps（2）~ （5）, selecting step（4）In obtained 2-3 age diamondback moths be placed in parasitic cage, and by step（5）Obtained parasitic wasp introduces and posts Raw cage carries out parasitism, and being transferred to other parasitic cages by parasitic 2-3 age diamondback moths is raised, and is collected bee cocoon, is cleared up parasitism cage, And repeat step above-mentioned steps（2）~（5）、（9）, continue generation breeding diamondback moth and parasitic wasp, until parasitic wasp raising reaches 1000 Only.
- 10. the method for rapid amplifying breeding brassicaceous vegetable lepidoptera pest parasitic wasp in interior according to claim 9, It is characterized in that：One section of adhesive tape is pasted in the parasitism cage, the projecting honey of at least one drop and an at least wet cotton are stained with adhesive tape, Honey supplies parasitic wasp feeding, and wet cotton is for parasitic wasp supplement moisture content.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197786A (en) * | 2018-10-31 | 2019-01-15 | 山西农业大学 | A kind of method of interior mass rearing semi-closure initial bow |
| CN109692798A (en) * | 2019-02-20 | 2019-04-30 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Drone screening plant and drone screening technique |
| CN111066689A (en) * | 2019-12-11 | 2020-04-28 | 浙江大学 | Device for preventing and controlling nest insects by using parasitic wasps and application thereof |
| CN114403100A (en) * | 2022-01-25 | 2022-04-29 | 福建农林大学 | Method for separating and collecting eggs and larvae of diamondback moth cocoon bee in vivo |
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| CN103503836A (en) * | 2013-08-22 | 2014-01-15 | 北京市农林科学院 | Plutella xylostella cotesia rubecula artificial propagation production method |
| CN104304183A (en) * | 2014-08-27 | 2015-01-28 | 浙江大学 | Artificial propagation and production method for diadromus collaris |
| JP2015091786A (en) * | 2013-09-30 | 2015-05-14 | 独立行政法人農業環境技術研究所 | Floral fragrance derived natural-enemy-insect attractant component and use thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109197786A (en) * | 2018-10-31 | 2019-01-15 | 山西农业大学 | A kind of method of interior mass rearing semi-closure initial bow |
| CN109692798A (en) * | 2019-02-20 | 2019-04-30 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Drone screening plant and drone screening technique |
| CN109692798B (en) * | 2019-02-20 | 2024-05-03 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Drone screening device and drone screening method |
| CN111066689A (en) * | 2019-12-11 | 2020-04-28 | 浙江大学 | Device for preventing and controlling nest insects by using parasitic wasps and application thereof |
| CN114403100A (en) * | 2022-01-25 | 2022-04-29 | 福建农林大学 | Method for separating and collecting eggs and larvae of diamondback moth cocoon bee in vivo |
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