CN103704178A - Method for adjusting diapause of Exorista civilis Rond - Google Patents
Method for adjusting diapause of Exorista civilis Rond Download PDFInfo
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Abstract
The invention discloses a method for adjusting the diapause of Exorista civilis Rond. The method comprises the following steps that (1) the Exorista civilis Rond with the diapause to be induced is placed in the environment with the conditions that the temperature is 17+/-1DEG C, and the photoperiod ranges from L8:D16 to L12:D12, and induction is carried out on the Exorista civilis Rond so that the Exorista civilis Rond can be in a diapause state; (2) the Exorista civilis Rond which is obtained in the step (1), and is in the diapause state is stored in the dark with the temperature of 4DEG C; (3) after storage in the step (2) is accomplished, the Exorista civilis Rond is placed in the environment with the conditions that the temperature is 23+/-1DEG C, and the photoperiod ranges from L14:D10 to L16:D8, and development is recovered. According to the method for adjusting the diapause of the Exorista civilis Rond, the diapause induction rate is 100%, the storage period after the diapause can be 120 days, and eclosion adult Exorista civilis Rond can be obtained after 20 days when the diapause is removed. The method for adjusting the diapause of the Exorista civilis Rond has important significance for large-scale expanding propagation of the Exorista civilis Rond, extension of the shelf life of Exorista civilis Rond products and the field prevention and control action time of the Exorista civilis Rond products, improvement of pest control efficiency, deepening of understanding about the natural enemy insect development mechanism, and exploration of the adaptive mechanism of insects to the environment and the evolution path of the insects.
Description
Technical field
The invention belongs to biological technical field, relate to a kind ofly regulating and controlling full skirt and chasing after the method for posting fly diapause.
Background technology
Natural enemy insect is a class parasitism or the insect that preys on other insects, and they are controlling the development of insect and are spreading in farmland, forest zone and pasture.China's natural enemy insect resource is very abundant, has the material base of strong Application and Development.By development natural enemy insect large-scale production technology; adopt indoor amount reproduction natural enemy insect method and a large amount of technology discharging; can increase the population quantity of the initial natural enemy in field; in conjunction with biopesticide and ecological technique; form comparatively complete techniques of pest control system; the access times and the consumption that substitute or reduce chemical pesticide, protection of the environment and bio-diversity, guarantee agricultural product production safety.Therefore, research natural enemy insect artificial feeding scale, commercialization production etc. have important application and promotional value.Carry out the dark people research of Insect Diapause, can be the mankind and can understand that insect grows and the rule of growth and decline, finally for nuisanceless improvement insect, utilize beneficial insect that scientific theory foundation is provided.
Beet webworm Loxostege sticticalis L. belongs to Lepidoptera, and Pyralidae class insect is a kind of universal farming and animal husbandry insect, because its host plant is of a great variety, is that a kind of intermittence is broken out and has a destructive migratory pest.In beet webworm parasitic insect, parasitic fly will, higher than parasitic wasp, play important regulating and controlling effect to host population to the parasitic rate of beet webworm.The abundantest with parasitic fly in the natural enemy monoid of beet webworm, be to control the key factor that harm and population dynamic occur beet webworm.
Full skirt chases after posts fly (Exorista civilis Rond.) genus diptera Larvaevoridae (Diptera:Tachinidae), it is holometabola parasitic fly, total ovum, larva, pupa and these four worm states (developmental stage) of adult in growth course, the development characteristics of each worm state is had nothing in common with each other, as ovum, pupa are not eaten motionless; Low instar larvae can only take food in pin main body, and mature larva climbs out of in worm pin main body, in 12h---24h, pupates, and enters the motionless state of not eating.Adult is had a flight performance wing, can circle in the air flexibly.
One of considerable advantage parasite on full skirt Zhui Jiyingshi China beet webworm larva.Although overcome the drawback that Traditional Man subculture is bred, can make full skirt chase after to post fly can carry out annual accumulation and breed.But adopt tradition to connect the method for generation breeding, fly easily degenerates, and be difficult for long-term preservation, to field, discharge and brought very large difficulty.By improving propagation technique, this fly of human-induced enters diapause status, can make storage life greatly extend, and therefore utilizes cryopreservation diapause worm cocoon technology, controls natural enemy development time, and this is of crucial importance for expanding numerous natural enemy insect meaning the anniversary.By hold each process of breeding from generation to generation and accurately target of prediction insect time of origin and there is quantity, when insect occurs, can discharge timely natural enemy insect, thereby reach the effect of control of insect.
Summary of the invention
The object of this invention is to provide and a kind ofly regulate and control full skirt and chase after the method for posting fly diapause.
Regulation and control full skirt provided by the present invention chases after the method for posting fly diapause, specifically comprises the steps:
(1) full skirt of pending diapause induction is chased after and post fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt that enters diapause state step (1) being obtained chases after to be posted fly and proceeds under 4 ℃ of temperature, dark condition and store;
(3) after step (2) is stored and to be finished, described full skirt is chased after and posts fly and proceed under the condition of 23 ± 1 ℃ of temperature, photoperiod L14:D10 to L16:D8, recover to grow.
A further object of the present invention is to provide a kind of full skirt and chases after and post fly diapause induction and storage procedures.
Full skirt provided by the present invention chases after posts fly diapause induction and storage procedures, specifically can comprise the steps:
(1) full skirt is chased after and post fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt that enters diapause state step (1) being obtained chases after to be posted fly and proceeds under 4 ℃ of temperature, dark condition and store.
Another object of the present invention is to provide a kind of full skirt and chases after and post fly diapause abductive approach.
Full skirt provided by the present invention chases after posts fly diapause abductive approach, specifically can comprise the steps: full skirt to chase after and post fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, and described full skirt is chased after post fly to enter diapause state.
Three kinds of methods that provide for the invention described above, in the present invention, in step (1), described temperature is specially 16 ℃, 17 ℃ or 18 ℃; The described photoperiod is specially L8:D16, L10:D14 or L12:D12.
Three kinds of methods that provide for the invention described above, in step (1), the intensity of illumination in described condition is 4000-5000LX; Relative air humidity in described condition is 60%-70%.
Three kinds of methods that provide for the invention described above, in step (1), the full skirt of described pending diapause induction chases after and posts fly is that full skirt chases after ovum or the larva of posting fly; Described full skirt is chased after and posts time that fly carries out diapause induction and at least contain described full skirt and chase after the larval phase of posting fly.
Concrete, in the present invention, in step (1), described " by the full skirt of pending diapause induction chase after post fly be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 induce " be: the described full skirt of ovum phase is chased after and posts fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, in pupa time or adult stage, described full skirt is chased after and post fly and take out from the condition of 17 ± 1 ℃ of described temperature, photoperiod L8:D16 to L12:D12.
In the step (2) of said method, the time of described storage can be in 120 days, was preferably in 60 days, as 30-60 days.Relative air humidity in described condition is 60%-70%, as 70%.
In the step (3) of said method, the time that described recovery is grown can be 20 days, and after 20 days, described full skirt chases after and posts fly and can turn into adult.Intensity of illumination in described condition is 5000LX; Relative air humidity in described condition is 60%-70%, as 70%.
In the present invention, described full skirt is chased after to post the host of fly when carrying out diapause induction, diapause maintenance or diapause and removing be beet webworm, as the beet webworm at the beginning of 4 age Mo～5 ages.
Regulation and control full skirt provided by the present invention chases after the method for posting fly diapause, and diapause inductivity is nearly 100%, and can reach 120 days the storage period after diapause, diapause remove after 20 day after tomorrow can obtain the one-tenth fly sprouting wings.The present invention not only contributes to promote full skirt to chase after in production practices and posts the vast propagation of fly, extends its product shelf phase and in the prevention and control action time in field, also contributes to grasp the development characteristics of natural enemy insect, improves control of insect efficiency.
Accompanying drawing explanation
Fig. 1 chases after the impact of posting fly diapause at temperature and photoperiod on full skirt.
When Fig. 2 is 16 ℃ and 18 ℃, full skirt chases after the critical photoperiod of the diapause induction of posting fly.
Embodiment
The experimental technique using in following embodiment if no special instructions, is conventional method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
For trying worm source:
Full skirt chases after posts fly: be recorded in " Xiang Hongyan; Liu Aiping; Gao Shujing; Xu Linbo; Liu Changzhong. beet webworm advantage parasite---full skirt chases after posts fly biological characteristic research [J]. Acta Prataculture; 2013,22 (3): 92-98 " in a literary composition, public Ke Cong grassland research institute, China Agriculture academy of sciences obtains.Full skirt chases after to be posted fly adult and raises in the disposable transparent water tumbler of 240ml, and rim of a cup covers with gauze, is sewed with rayon balls on gauze, and uses 15%(volume fraction) hydromel soaks.
Beet webworm: be recorded in " Wu Jinhua, Liu Aiping etc. the impact [J] of artificial feed on beet webworm digestive enzyme activity and carboxy-lesterase mrna expression amount. meadow journal; 2012; 20(6):: 169-1174 " in a literary composition, public Ke Cong grassland research institute, China Agriculture academy of sciences obtains.Beet webworm ovum is placed in and is equipped with the glass culture dish that the radius of filter paper is 15cm, and wherein filter paper should keep moistening.After beet webworm hatching, with fresh goosefoot leaf or the cloverleaf beet webworm of feeding, until beet webworm larva while being 3 age, turning larva in the plastic casing of length * wide=15cm * 11cm and raises, 3-5 instar larvae environment of living in remains on 60% left and right.Feed 1-2 every day, and change in time feed and screen out worm excrement.In order to reduce the death of larva, should reduce while changing blade as far as possible the brush of larva is hindered or stirred, adopt shake gently or concussion blade to make larva self falling to fresh blade.
Test apparatus and material
Temperature and photoperiod on full skirt chase after post fly diapause induction impact test all in climatic cabinate (SPX-300I-G type, Shanghai Medical Equipment Plant of Bo Xun Industrial Co., Ltd.) in, carry out, climatic cabinate temperature step-up error is ± 1 ℃, and relative moisture step-up error is ± 5%.Beet webworm larva and full skirt chase after the raising of posting fly all to carry out in self-control dependent insect cage.Separately need flat based tubes (6cm * 1.5cm), mercurial thermometer, writing brush, absorbent cotton, elastic tape, pallet, culture dish, preservative film, vermiculite etc. are some.
The experimental technique using in following embodiment if no special instructions, is conventional method.
When following embodiment implementation data is analyzed, all utilize DPS to carry out variance analysis, significance test of difference adopts Duncans duncan's new multiple range method.
In following embodiment, photoperiod Lx:Dy represents light irradiation time x hour every day, dark duration y hour, x+y=24.Photoperiod LL represents light irradiation time 24 hours every day, dark duration 0 hour; Photoperiod DD represents light irradiation time 0 hour every day, dark duration 24 hours.
Following embodiment all adopts international parasitic fly diapause criterion, and basic principle is based on the rule of effective temperature summation, when certain worm state of certain parasitic fly remains unfulfilled growth in three times of times at the shortest development duration, assert it is to enter diapause status.More concrete, in the present invention, judge that full skirt chases after and post fly and whether enter diapause status and determine as follows: if full skirt chases after and posts this worm state of fly " larva " remain unfulfilled growth within three times of times (27 days) of the shortest development duration, when ovum is hatched into larva, in 27 days, full skirt chases after the larva of posting fly and fails to enter " pupa " this worm state, assert that full skirt chases after to post fly and enter diapause status.
Embodiment 1, full skirt chase after the diapause induction of posting fly
One, full skirt chases after and posts fly diapause temperature and photoperiodic definite
4 different temperatures (14 ℃, 17 ℃, 21 ℃ and 25 ℃) and 5 different photoperiods (L8:D16, L10:D14, L12:D12, L14:D10, L16:D8) are set, totally 20 processing, each processes 30 healthy and strong beet webworm larvas (host) at the beginning of 4 age Mo～5 ages, repeats 3 times.Full skirt after mating 1d is chased after and posts the female fly of fly and beet webworm larva is together placed in insect box, put into the climatic cabinate of different photoperiods and Temperature Treatment (wherein, intensity of illumination during illumination is that 4000～5000LX, relative air humidity are 60%-70%), full skirt is chased after and posts fly and carry out diapause induction (diapause induction from " ovum " this worm state), induction 10-12 days.After having induced, proceed to normal raising condition, in the illumination box that 23 ℃, L16:D8, relative air humidity are 60%, raise, each processed group full skirt of observed and recorded chases after the diapause situation of posting fly, and polypide developmental state.
Result is as shown in table 1 and Fig. 1, and photoperiod and temperature are that full skirt chases after the Major environment impacts factor of posting fly diapause induction.Being temperature or photoperiod chases after and posts fly diapause rate and all have significant impact full skirt.In the time of 17 ℃, diapause rate is the highest, and 17 ℃ is best diapause temperature.Lower than 17 ℃, larva activity is slow, stiff.Death toll increases.In the time of 21 ℃, along with the increase of light application time, diapause rate declines gradually; In the time of 25 ℃, can not induce diapause.Visible, low temperature, short illumination are that induction full skirt chases after the principal element of posting fly diapause, and temperature plays a leading role, and the photoperiod only works in certain temperature range.
Under table 1 indoor conditions, temperature and photoperiod chase after the impact of posting fly diapause on full skirt
Note: data in table (mean value ± standard error) are newly answered range test through DuncanShi, after each row of data, different capitalizations represent difference extremely significantly (P < 0.01); Different lowercase alphabets show significant difference (P < 0.05).The cocoon that " cocoon " in table in " cocoon number " tied after pupating for beet webworm, investigates cocoon number, because full skirt chases after, to post fly be to grow in pin main body, finally sprouts wings into adult, therefore observed, is beet webworm cocoon.
Two, full skirt chases after and posts determining of fly diapause induction critical photoperiod
Under 16 ℃ and 18 ℃ of conditions, establish respectively LL, L2:D22, L4:D20, L6:D18, L8:D16, L10:D14, L12:D12, L14:D10, L16:D8, L18:D6, L20:D4, L22:D2, a DD13 different photoperiod and process, amount to 26 processing.
Select the healthy and strong beet webworm larva at the beginning of 4 age Mo～5 ages, put on the fresh alfalfa leaf or goosefoot leaf that connects fly case (40cm * 30cm * 25cm) bottom, full skirt after mating 1d being chased after and posts the female fly of fly and put into and connect fly case, will be soaked with 15%(volume fraction) rayon balls of the hydromel end face that hangs over fly case chases after the food source of posting fly adult as full skirt.After 24h, remove full skirt and chase after and post fly, beet webworm is proceeded to respectively in vial and continues to raise with fresh alfalfa leaf or goosefoot leaf, every bottle of 50 incubators that are placed in respectively different disposal.Illumination box environment set temperature error ± 1 ℃, intensity of illumination 4000～5000LX, relative air humidity 60%-70%.Each is processed and repeats 3 times.Observe full skirt under the different photoperiods of record and chase after the diapause situation of posting fly, thereby definite full skirt chases after, post fly diapause induction critical photoperiod.
Result as shown in Figure 2, can find out, light application time is greater than 16h(L>16h) time, 100% full skirt chases after to be posted fly individuality and all can grow, and does not occur diapause situation.The ratio of high diapause only appears between short-day 8～12h; Short-day below 8h, the ratio of diapause obviously declines with the shortening at sunshine, Quan under the sunshine and dark of 2h every day, all ontogeny.Critical day-length is 12.53h in the time of 16 ℃ as seen from the figure, in the time of 18 ℃, is 11.12h.
Three, full skirt chases after and posts determining of fly diapause regulation and control life stages sensitive
According to the result of the light temperature diapause induction in step 1 and step 2, by 16 ℃ of temperature, the short illumination of photoperiod L10: D14(, S) as induction diapause condition, by 16 ℃ of temperature, the long illumination of photoperiod L16: D8(, L), as non-diapause condition, carry out following life stages sensitive determination test.Intensity of illumination in process of the test is 5000LX, and relative air humidity is 60%.
Select the healthy and strong beet webworm larva at the beginning of 4 age Mo～5 ages, put on the fresh alfalfa leaf or goosefoot leaf that connects fly case (40cm * 30cm * 25cm) bottom, full skirt after mating 1d being chased after and posts the female fly of fly and put into and connect fly case, will be soaked with 15%(volume fraction) rayon balls of the hydromel end face that hangs over fly case chases after the food source of posting fly adult as full skirt.After 24h, remove full skirt and chase after and post fly, beet webworm is proceeded to respectively in vial and continues to raise with fresh alfalfa leaf or goosefoot leaf, 30 every bottle.Full skirt is chased after and posts the fly worm phase and be divided into ovum, low instar larvae (1-3 instar larvae), high instar larvae (4-5 instar larvae) and adult four-stage, from the ovum phase, by different treatment combination designing requirements (in Table 2), will alternately be placed in corresponding photoperiod environment for examination worm in time.Observe record different disposal full skirt and chase after the diapause situation of posting fly, thereby determine that full skirt chases after the sensitive period of posting fly diapause induction.
Result is as shown in table 2, it is the crucial life stages sensitive of its diapause that known full skirt chases after the larval phase of posting fly, to the sensitive period of photoperiod irritant reaction, be larval phase, low instar larvae (1-3 instar larvae) or high instar larvae (4-5 instar larvae) all can be experienced the inducing action of short illumination to diapause.Ovum phase and adult stage are insensitive to the diapause inducing action of short illumination.Only have larva to be under short illumination condition, larva just can enter diapause; Larval phase, is accepted after short light treatment continuously, and its diapause rate obviously increases, and shows that photoperiod stimulation has summation.To the diapause of larva, there is also to have certain facilitation in the short illumination experience of ovum phase.
Table 2 full skirt chases after posts the sensitive period that fly different developmental phases is experienced diapause signal
| Process | The ovum phase | 1-3 instar larvae | 4-5 instar larvae | Just incubate adult | Observe borer population (head) | Diapause borer population (head) | Diapause rate (%) |
| 1 | S | L | L | L | 47 | 3 | 6.4 |
| 2 | L | S | L | L | 53 | 30 | 56.6 |
| 3 | L | L | S | L | 49 | 25 | 51.0 |
| 4 | L | L | L | S | 46 | 2 | 4.3 |
| 5 | L | L | L | L | 55 | 4 | 7.3 |
| 6 | S | S | L | L | 51 | 25 | 49.0 |
| 7 | S | S | S | L | 41 | 31 | 75.6 |
| 8 | S | S | S | S | 53 | 50 | 94.3 |
| 9 | L | S | S | S | 51 | 47 | 92.2 |
| 10 | L | L | S | S | 49 | 29 | 59.2 |
| 11 | L | S | S | L | 48 | 41 | 85.4 |
Note: long illumination (L, the photoperiod is L16:D8) and short illumination (S, the photoperiod is L10:D14).
1, full skirt chases after the eclosion rate of posting fly
The full skirt of the diapause state that the method that adopts embodiment 1 is obtained chases after to be posted fly larva (parasitizing in the beet webworm body of having pupated) and is positioned in the plastic containers that fill moist fine sand, then put it in 4 ± 2 ℃, refrigerator, under full dark condition, preserve, the moisturizing of spraying water every day, maintains relative air humidity 70%.Regularly there is diapause state full skirt to chase after parasitism to post the beet webworm cocoon of fly larva to take out from refrigerator to put into the incubator of 23 ± 1 ℃ of temperature, photoperiod L16: D8 to carry out full skirt and chase after the emergence of posting fly, observe and add up the eclosion rate that refrigerates parasitic fly after different time.8 processing (8 cold preservation times, in Table 3) are established in experiment altogether, and each processes 50-70 parasitism has diapause state full skirt to chase after the beet webworm cocoon of posting fly larva.Simultaneously not refrigerate beet webworm cocoon that the parasitism of processing has non-diapause state full skirt to chase after to post a fly larva (CK) in contrast. experiment repeats 5 times, results averaged.
2, the full skirt after emergence chases after the life-span of posting fly
Full skirt after the emergence that step 1 is obtained chases after to be posted fly and becomes fly to put into insect box, every box is introduced a pair of full skirt and is chased after and post fly (the 1 male fly in female fly+1), hydromel nutrition as a supplement with mass fraction 15%, mating 24h under the condition of 23 ℃ of temperature, photoperiod L16: D8, then inoculate, be about to full skirt after mating 1d and chase after and post the female fly of fly and parasitize beet webworm larva.Repeat 3 times.30 4-5 beet webworm in age larvas of every pair of fly inoculation are parasitic for female fly, take out male and female fly and continue inoculation, until the death of male and female fly after 24h.Statistics full skirt chases after the life-span of posting the one-tenth fly after fly sprouts wings.
3, result
Result is as shown in table 3, finds that the long full skirt that can significantly reduce of storage time chases after the eclosion rate of posting fly.Without the full skirt storing, chase after that to post fly eclosion rate be 85.2%.Passing along with the storage time, full skirt chases after the eclosion rate of posting fly obviously to be reduced, and there is utmost point significant difference (p < 0.01) between the eclosion rate in different storage times of " 0-150 days ", female, male fly life-span and control group have significant difference (P>0.05), illustrate that parasitic beet webworm cocoon low temperature storage 30d, the 60d that has diapause state full skirt to chase after to post a fly larva chases after full skirt to post fly eclosion rate, life-span, there is no considerable influence.Above result shows have diapause state full skirt to chase after to post the beet webworm cocoon of fly larva to do short-term to parasitism, and to store 30-60d comparatively suitable
Table 3 low temperature storage chases after the impact of posting fly on full skirt
Note: data in table (mean value ± standard error) are newly answered range test through DuncanShi, after each row of data, different capitalizations represent difference extremely significantly (P < 0.01); Different lowercase alphabets show significant difference (P < 0.05).
Embodiment 3, full skirt chase after the diapause releasing of posting fly
The full skirt of the diapause state obtaining for the method that adopts embodiment 1 chases after posts fly, can be by thermal stimulus, and termination of diapause state, enters development process, and concrete measure is as follows:
There is diapause state full skirt to chase after to post the beet webworm cocoon of fly larva to be placed in 23 ℃, the environment of photoperiod L14:D10, luminous intensity 5000Lx parasitism, spray water every day one time, keep certain humidity (RH is 70%), after 20 days, can make full skirt chase after and post fly larva termination of diapause state, all sprout wings and into fly very soon.
Wherein, induction time is determined for 20 days as follows:
The beet webworm pupa that the method that adopts embodiment 2 is stored to parasitism after 60 days and have diapause state full skirt to chase after to post fly larva under full dark, 4 ℃ of conditions proceeds to incubator (the luminous intensity 5000Lx of 23 ℃ of temperature, photoperiod L14: D10, RH is 70%) in carry out diapause releasing, the emergence time of statistics parasitic fly, adopt 50-60 parasitism to have diapause state full skirt to chase after to post the beet webworm cocoon of fly larva to test, establish altogether and repeat experiment for 3 times.Result shows, from the beet webworm cocoon that parasitism is had diapause state full skirt chase after to post fly larva proceeds to when starting to carry out diapause under 23 ℃ of conditions and removing, after approximately 20 days, all sprouts wings and into fly very soon, termination of diapause state.Repeat experimental result indifference three times.
Claims (7)
1. regulate and control full skirt and chase after a method of posting fly diapause, comprise the steps:
(1) full skirt of pending diapause induction is chased after and post fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt that enters diapause state step (1) being obtained chases after to be posted fly and proceeds under 4 ℃ of temperature, dark condition and store;
(3) after step (2) is stored and to be finished, described full skirt is chased after and posts fly and proceed under the condition of 23 ± 1 ℃ of temperature, photoperiod L14:D10 to L16:D8, recover to grow.
2. full skirt chases after and posts fly diapause induction and a storage procedures, comprises the steps:
(1) full skirt is chased after and post fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, described full skirt is chased after post fly to enter diapause state;
(2) the described full skirt that enters diapause state step (1) being obtained chases after to be posted fly and proceeds under 4 ℃ of temperature, dark condition and store.
3. full skirt chases after and posts a fly diapause abductive approach, comprises the steps: full skirt to chase after and post fly and be placed under the condition of 17 ± 1 ℃ of temperature, photoperiod L8:D16 to L12:D12 and induce, and described full skirt is chased after post fly to enter diapause state.
4. according to arbitrary described method in claim 1-3, it is characterized in that: in step (1), the full skirt of described pending diapause induction chases after and posts fly is that full skirt chases after ovum or the larva of posting fly; Described full skirt is chased after and posts time that fly carries out diapause induction and at least contain described full skirt and chase after the larval phase of posting fly.
5. according to arbitrary described method in claim 1-4, it is characterized in that: in step (2), the time of described storage is in 120 days.
6. method according to claim 5, is characterized in that: in step (2), the time of described storage is in 60 days.
7. according to arbitrary described method in claim 1-6, it is characterized in that: in step (3), the time that described recovery is grown is 20 days.
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| CN104351140A (en) * | 2014-09-24 | 2015-02-18 | 中国农业科学院植物保护研究所 | Leis dimidiata diapause relieving method |
| CN105379685A (en) * | 2015-11-25 | 2016-03-09 | 云南农业大学 | Method for preserving exorista japonica townsend pupae |
| CN105660541A (en) * | 2016-02-15 | 2016-06-15 | 山东省农业科学院植物保护研究所 | Method for inducing reproductive diapause of spotted wing drosophila |
| CN109548745A (en) * | 2018-12-07 | 2019-04-02 | 广东省生物资源应用研究所 | A kind of method of low temperature induction tortoise ovum diapause |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003088274A (en) * | 2001-09-19 | 2003-03-25 | National Institute Of Agrobiological Sciences | Method for introducing polynucleotide to silkworm producing dormant egg |
| UA25135U (en) * | 2007-03-27 | 2007-07-25 | Univ Nat Agrarian | Method to form a wintering reserve of species of the genus trichogramma |
| JP2008113605A (en) * | 2006-11-06 | 2008-05-22 | Nakamura Kazuo | Method for breeding, supplying and raising japanese indigenous bumblebees |
| CN102206263A (en) * | 2011-03-23 | 2011-10-05 | 吉林省蚕业科学研究院 | Tussah antibiotic extraction method based on tussah pupa in vivo induction |
| CN102771448A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Natural enemy insect (coccinella septempunctata) diapause induction method |
| CN102771445A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Diapause induction and cancellation method of natural enemy insect |
| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
-
2013
- 2013-12-19 CN CN201310703160.8A patent/CN103704178B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003088274A (en) * | 2001-09-19 | 2003-03-25 | National Institute Of Agrobiological Sciences | Method for introducing polynucleotide to silkworm producing dormant egg |
| JP2008113605A (en) * | 2006-11-06 | 2008-05-22 | Nakamura Kazuo | Method for breeding, supplying and raising japanese indigenous bumblebees |
| UA25135U (en) * | 2007-03-27 | 2007-07-25 | Univ Nat Agrarian | Method to form a wintering reserve of species of the genus trichogramma |
| CN102206263A (en) * | 2011-03-23 | 2011-10-05 | 吉林省蚕业科学研究院 | Tussah antibiotic extraction method based on tussah pupa in vivo induction |
| CN102771448A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Natural enemy insect (coccinella septempunctata) diapause induction method |
| CN102771445A (en) * | 2012-08-07 | 2012-11-14 | 中国农业科学院植物保护研究所 | Diapause induction and cancellation method of natural enemy insect |
| CN103141443A (en) * | 2013-03-08 | 2013-06-12 | 中国农业科学院草原研究所 | Method for regulating and controlling diapause of agryponflexorius |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104351140A (en) * | 2014-09-24 | 2015-02-18 | 中国农业科学院植物保护研究所 | Leis dimidiata diapause relieving method |
| CN105379685A (en) * | 2015-11-25 | 2016-03-09 | 云南农业大学 | Method for preserving exorista japonica townsend pupae |
| CN105379685B (en) * | 2015-11-25 | 2017-10-10 | 云南农业大学 | A kind of method of the Japanese Exorista civilis pupa of preservation |
| CN105660541A (en) * | 2016-02-15 | 2016-06-15 | 山东省农业科学院植物保护研究所 | Method for inducing reproductive diapause of spotted wing drosophila |
| CN105660541B (en) * | 2016-02-15 | 2019-03-01 | 山东省农业科学院植物保护研究所 | A method of induction spotted wing drosophila reproductive diapause |
| CN109548745A (en) * | 2018-12-07 | 2019-04-02 | 广东省生物资源应用研究所 | A kind of method of low temperature induction tortoise ovum diapause |
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