CN105379685A - Method for preserving exorista japonica townsend pupae - Google Patents
Method for preserving exorista japonica townsend pupae Download PDFInfo
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- CN105379685A CN105379685A CN201510827391.9A CN201510827391A CN105379685A CN 105379685 A CN105379685 A CN 105379685A CN 201510827391 A CN201510827391 A CN 201510827391A CN 105379685 A CN105379685 A CN 105379685A
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- host
- exorista
- townsend
- japonica
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention relates to a method for preserving exorista japonica townsend pupae and belongs to the technical field of insect breeding. After exorista japonica townsend parasitizes a host, the host is fed under the temperature of 22 DEG C-30 DEG C until the exorista japonica townsend pupates, the feeding temperature is decreased to 15-18 DEG C at the rate of 0.2-0.5 DEG C/h, and the environment is maintained until exorista japonica townsend larvae in the body of the host creep out of the body of the host and pupate; the exorista japonica townsend pupae creeping out of the body of the host are collected and wrapped with absorbent cotton, quartz sand with the water content accounting for 4-7% by weight is used for burying pupa bodies until the pupa bodies are buried by 0.5-2 mm, the temperature is degreased to 5-8 DEG C at the rate of 2-5 DEG C/h and then maintained for 23-25 h, the temperature continues to be decreased to minus 2-2 DEG C at the rate of 2-5 DEG C/h, and preservation is carried out. The exorista japonica townsend pupae can resuscitate according to specific conditions after preservation and become adults through natural eclosion. The cold storage preservation time of the pupae is prolonged to 300-480 days, and the eclosion succeeding rate is also increased.
Description
Technical field
The invention belongs to cultured insect technical field.
Background technology
Japan chases after and posts fly (ExoristajaponicaTownsend) is (diptera Diptera Larvaevoridae Tachinidae) large ovum type parasitic fly, the ovum not yet completing embryonic development is originated in host's body surface by female fly, pierce in host larva body cavity after larvae hatch, battalion's obligatory parasitism, finally the stage in pupa time get out host host, the larva of its host mainly lepidopterous insects.Japan chases after and posts fly is a kind of biological control type insect being widely used in the lepidoptera pests such as Lasiocampidae, Noctuidae, Pyralidae, Hesperiidae, Arctiidae, and it has quite huge agricultural economy potentiality.
Obtain the patent that notification number is CN102106324B before applicant, content utilizes three-spotted plusia to breed Japan for vector to chase after and post fly.A step in this patented method is that preservation chases after and posts fly pupa, and way is preserved 15 days at 6 ~ 7 degrees Celsius, can the holding time shorter.Practice shows, this is existing chases after and post fly pupa preservation technology and require further improvement.
Summary of the invention
The object of the present invention is to provide a kind of preservation Japan to chase after the method for posting fly pupa, to extend the holding time of pupa, and improve the emergence success rate of fly pupa and chase after the vigor posting fly after sprouting wings.
The inventive method is according to the following steps:
Precooling: Japan chases after that to post fly parasitic to host, raising host to pupating, being cooled to raising temperature 15 ~ 18 DEG C with 0.2 ~ 0.5 DEG C/h at 22 DEG C ~ 30 DEG C, keeps this environment until chasing after in pin main body posts fly larva gets out in body and pupate;
Refrigeration: collect chasing after of getting out in pin main body and post fly pupa, wrap up with absorbent cotton, with wet weight than being that the quartz sand landfill of 4 ~ 7% is to not having pupal cell 0.5 ~ 2mm, 5 DEG C ~ 8 DEG C are cooled to the speed of 2 ~ 5 DEG C/h, keep 23 ~ 25 hours, continue to be cooled to-2 DEG C ~ 2 DEG C with the speed of 2 ~ 5 DEG C/h, carry out preservation;
Recovery: chasing after of above-mentioned preservation is posted fly pupa and be warming up to 6 ~ 8 DEG C with the speed of 0.8 ~ 1.2 DEG C/h, keep 23 ~ 25 hours, 10 ~ 12 DEG C are warming up to again with 0.8 ~ 1.2 DEG C/h speed, discard quartz sand, be warming up to normal temperature with 0.8 ~ 1.2 DEG C/h speed again and keep relative moisture for 40% ~ 80%, again the pupa of recovery is placed in natural environment relaying supervention and educates 3 ~ 5 days, adult can be turned into by nature.
Said Japan chases after and posts the parasitic method to host of fly is prior art, such as patented technology CN102106324B.
The inventive method chases after posts fly pupa and can refrigerate 300 ~ 480 days.
When other conditions are identical, use the store method in method for preserving of the present invention and patent document CN102106324B, chase after the eclosion rate comparative test result of posting fly pupa as follows:
| The preservation time | The eclosion rate of the inventive method | The eclosion rate of method for preserving in CN102106324B |
| 200 days | 99％ | 99％ |
| 300 days | 92％ | 74％ |
| 400 days | 89％ | 12％ |
| 450 days | 83％ | 1％ |
| 500 days | 76％ | 0％ |
Good effect of the present invention: Japan can be chased after the pupa stored refrigerated time of posting fly under the low temperature of-2 DEG C ~ 2 DEG C is increased to 300 ~ 480 days, also improve Japan to chase after and post fly and change into power at long-term frozen aftershaft, this can improve production ability of regulation and control greatly, reduces transport difficulty.
Embodiment
Embodiment 1:
Japan is chased after and posts fly employing patented technology CN102106324B method parasitism to host, at 22 DEG C, host is raised to pupating, feeding environment is lowered the temperature 25 little of raising temperature 18 DEG C with 0.2 DEG C/h, keep 18 DEG C of environment 6 days, be collected in (namely get out in pin main body) around host post fly pupa, wrap up with absorbent cotton, with the quartz sand landfill of water content 4% to not having pupal cell 0.5mm, 5 DEG C are cooled to 2 DEG C/h speed, refrigeration 24 as a child continued to be cooled to-2 DEG C with 2 DEG C/h, preserve and take out after 1 year, 6 DEG C are warming up to the speed of 0.8 DEG C/h, keep 24 hours, continue to continue to be warming up to 10 DEG C with 0.8 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 0.8 DEG C/h speed, humidity remains 40%, be placed in natural environment relaying supervention and educate 4 days, emerging adult rate is 93.1%.
Embodiment 2:
Japan is chased after and posts fly employing patented technology CN102106324B method parasitism to host, at 22 DEG C, host is raised to pupating, feeding environment is lowered the temperature 14 little of raising temperature 18 DEG C with 0.5 DEG C/h, keep 18 DEG C of environment 8 days, be collected in (namely get out in pin main body) around host post fly pupa, wrap up with absorbent cotton, with the quartz sand landfill of water content 6% to not having pupal cell 0.5mm, 8 DEG C are cooled to 2 DEG C of speed, refrigeration 24 as a child continued to be cooled to-2 DEG C with 2 DEG C, preserve and take out after 480 days, 8 DEG C are warming up to the speed of 1.2 DEG C/h, keep 24 hours, continue to continue to be warming up to 10 DEG C with 1.2 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 1.2 DEG C/h speed, humidity remains 80%, be placed in natural environment relaying supervention and educate 3 days, emerging adult rate is 96.7%.
Embodiment 3:
Japan is chased after and posts fly employing patented technology CN102106324B method parasitism to host, at 30 DEG C, host is raised to pupating, feeding environment is lowered the temperature 30 little of raising temperature 15 DEG C with 0.5 DEG C/h, keep 15 DEG C of environment 9 days, be collected in (namely get out in pin main body) around host post fly pupa, wrap up with absorbent cotton, with the quartz sand landfill of water content 6% to not having pupal cell 2mm, 5 DEG C are cooled to 2 DEG C/h speed, refrigeration 24 as a child continued to be cooled to 2 DEG C with 2 DEG C/h, preserve and take out after 300 days, 6 DEG C are warming up to the speed of 0.8 DEG C/h, keep 24 hours, continue to continue to be warming up to 10 DEG C with 0.8 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 0.8 DEG C/h speed, humidity remains 80%, be placed in natural environment relaying supervention and educate 5 days, emerging adult rate is 89.4%.
Embodiment 4:
Japan is chased after and posts fly employing patented technology CN102106324B method parasitism to host, at 30 DEG C, host is raised to pupating, feeding environment is lowered the temperature 24 little of raising temperature 15 DEG C with 0.5 DEG C/h, keep 15 DEG C of environment 11 days, be collected in around host and (namely get out in pin main body, ) post fly pupa, wrap up with absorbent cotton, with the quartz sand landfill of water content 4% to not having pupal cell 0.5mm, 8 DEG C are cooled to 5 DEG C/h speed, refrigeration 24 as a child continued to be cooled to 2 DEG C with 5 DEG C/h, preserve and take out after 300 days, 6 DEG C are warming up to the speed of 1.2 DEG C/h, keep 24 hours, continue to continue to be warming up to 12 DEG C with 1.2 DEG C/h speed, discard quartz sand, continue to be warming up to normal temperature with 1.2 DEG C/h speed, humidity remains 40%, be placed in natural environment relaying supervention and educate 4 days, emerging adult rate is 92.6%.
Claims (1)
1. preservation Japan chases after a method of posting fly pupa, it is characterized in that according to the following steps:
Precooling: Japan chases after that to post fly parasitic to host, raising host to pupating, being cooled to raising temperature 15 ~ 18 DEG C with 0.2 ~ 0.5 DEG C/h at 22 DEG C ~ 30 DEG C, keeps this environment until chasing after in pin main body posts fly larva gets out in body and pupate;
Refrigeration: collect chasing after of getting out in pin main body and post fly pupa, wrap up with absorbent cotton, with wet weight than being that the quartz sand landfill of 4 ~ 7% is to not having pupal cell 0.5 ~ 2mm, 5 DEG C ~ 8 DEG C are cooled to the speed of 2 ~ 5 DEG C/h, keep 23 ~ 25 hours, continue to be cooled to-2 DEG C ~ 2 DEG C with the speed of 2 ~ 5 DEG C/h, carry out preservation;
Recovery: chasing after of above-mentioned preservation is posted fly pupa and be warming up to 6 ~ 8 DEG C with the speed of 0.8 ~ 1.2 DEG C/h, keep 23 ~ 25 hours, 10 ~ 12 DEG C are warming up to again with 0.8 ~ 1.2 DEG C/h speed, discard quartz sand, be warming up to normal temperature with 0.8 ~ 1.2 DEG C/h speed again and keep relative moisture for 40% ~ 80%, again the pupa of recovery is placed in natural environment relaying supervention and educates 3 ~ 5 days, adult can be turned into by nature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510827391.9A CN105379685B (en) | 2015-11-25 | 2015-11-25 | A kind of method of the Japanese Exorista civilis pupa of preservation |
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|---|---|---|---|
| CN201510827391.9A CN105379685B (en) | 2015-11-25 | 2015-11-25 | A kind of method of the Japanese Exorista civilis pupa of preservation |
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| CN105379685A true CN105379685A (en) | 2016-03-09 |
| CN105379685B CN105379685B (en) | 2017-10-10 |
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| CN201510827391.9A Expired - Fee Related CN105379685B (en) | 2015-11-25 | 2015-11-25 | A kind of method of the Japanese Exorista civilis pupa of preservation |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612946A (en) * | 2019-10-23 | 2019-12-27 | 广西壮族自治区林业科学研究院 | Method and device for breeding dastarcus helophoroides larvae |
| CN111264473A (en) * | 2020-02-18 | 2020-06-12 | 广西壮族自治区林业科学研究院 | Method for breeding Exorista bigelovii by utilizing galleria mellonella |
| CN113575520A (en) * | 2021-08-11 | 2021-11-02 | 浙江省农业科学院 | Fly pupa preservation method based on effective accumulated temperature accumulation value |
| WO2023137772A1 (en) * | 2022-01-24 | 2023-07-27 | 苏州大学 | Method for controlling exorista japonica population |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109757441B (en) * | 2019-03-13 | 2021-05-18 | 中国农业科学院植物保护研究所 | Method for storing arma chinensis |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612946A (en) * | 2019-10-23 | 2019-12-27 | 广西壮族自治区林业科学研究院 | Method and device for breeding dastarcus helophoroides larvae |
| CN111264473A (en) * | 2020-02-18 | 2020-06-12 | 广西壮族自治区林业科学研究院 | Method for breeding Exorista bigelovii by utilizing galleria mellonella |
| CN111264473B (en) * | 2020-02-18 | 2021-09-28 | 广西壮族自治区林业科学研究院 | Method for breeding Exorista bigelovii by utilizing galleria mellonella |
| CN113575520A (en) * | 2021-08-11 | 2021-11-02 | 浙江省农业科学院 | Fly pupa preservation method based on effective accumulated temperature accumulation value |
| WO2023137772A1 (en) * | 2022-01-24 | 2023-07-27 | 苏州大学 | Method for controlling exorista japonica population |
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| CN105379685B (en) | 2017-10-10 |
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