CN102106324A - Method for breeding exorista japonica townsend by using argyrogramma agnate as intermediate host - Google Patents
Method for breeding exorista japonica townsend by using argyrogramma agnate as intermediate host Download PDFInfo
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- CN102106324A CN102106324A CN 201110042453 CN201110042453A CN102106324A CN 102106324 A CN102106324 A CN 102106324A CN 201110042453 CN201110042453 CN 201110042453 CN 201110042453 A CN201110042453 A CN 201110042453A CN 102106324 A CN102106324 A CN 102106324A
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Abstract
The invention relates to a method for breeding exorista japonica townsend by using argyrogramma agnate as an intermediate host, belonging to the field of biological prevention and control technology. The method for breeding exorista japonica townsend by using argyrogramma agnate as the intermediate host comprises the following steps: feeding the argyrogramma agnate with artificial feed indoors; breeding the exorista japonica townsend by using the argyrogramma agnate as the intermediate host; and storing and releasing the pupas of the exorista japonica townsend. The method provided by the invention has the advantages that: the exorista japonica townsend can be uninterruptedly bred in a large scale and used for preventing and controlling lepidoptera pests in fields; and the application of biological prevention and control on various kinds of crops is promoted, thus the use of chemical pesticide is greatly reduced, the pesticide residue is reduced, and the manpower and material resources are saved.
Description
Technical field:
The present invention relates to the biological control technical field, specifically utilize three-spotted plusia to breed Japan and chase after the method for posting fly for vector.
Background technology:
It is the important technique measure of biological control of insect pests that the artificial breeding of natural enemy insect and release are used, and wherein the artificial breeding of parasite and utilization occupy considerable status.China has numerous available natural enemy insect resources, because the approaches and methods of breeding of unsuitable alternative host and corresponding science has only the part natural enemy insect can carry out artificial breeding and utilization.Japan chases after that to post fly very wide in the distribution of China, no diapause phenomenon in the history of life, host's spectrum is wide in the extreme again, as can parasitic fall webworm, lepidoptera pest on the agriculture and forestry such as mythimna separata, dendrolimus punctatus, cotton bollworm, rice plant skipper, corn borer, willow moth, imported cabbageworm, gypsymoth, rice caterpillar, the black poison moth of tea, also than higher, is a kind of parasitic insect that well can be used for biological control at the natural parasitic rate in field.
Domestic once had can use mythimna separata to carry out Japan to chase after and post the relevant report that fly breeds, but it is because host restricted, does not solve bottlenecks such as not posting the parasitic and release at any time of fly anniversary.
By literature search, do not see the open report identical with the present invention.
Summary of the invention:
The object of the present invention is to provide a kind of cost low, can biological control the field lepidoptera pest utilize three-spotted plusia to breed Japan to chase after the method for posting fly for vector.
Of the present inventionly utilize three-spotted plusia to chase after the concrete steps of the method for posting fly as follows for vector is bred Japan:
(1) raises the vector three-spotted plusia
From the healthy larva of field acquisition three-spotted plusia, at 25 ℃ of room temperatures, relative moisture 75%-90%, illumination condition L: D=16: under 8 the condition, raise with the artificial feed that market is bought, larva is gone through phase 10-15 days, 6-9 days pupa time, adult stage 11-15 days;
(2) the fly source breeds
Chased after the three-spotted phytometra larvae of posting the parasitic mistake of fly by Japan from field acquisition, also raise to pupating by the condition of above-mentioned (1) step, in three-spotted plusia pupa time, near its pupa, gather Japan and chase after the pupa of posting fly, the fly pupa collected put into dependent insect cage, after treating adult eclosion, put into percent by volume in the cage and be 0.5% hydromel and mass percent and be 0.2% milk power solution, supplement the nutrients for adult;
Treating that three-spotted phytometra larvae that above-mentioned (1) step breeds is most is 4-5 during age, puts it in the inoculation cage, inserts after the mating Japan of 6-7 days simultaneously and chases after and post the fly adult, is allowed to condition at the larva polypide and goes up ovipositing hosts; Fly moth part ratio is 1: 10, and at 25 ℃ of room temperatures, relative moisture 75%-90%, illumination condition L: D=16: under 8 the condition, raise with the artificial feed that market is bought, the larva of three-spotted plusia pupated after 1-4 days;
(3) preservation in fly source and release period
A. the fly pupa of collecting by above-mentioned steps was preserved 15 days under 6-7 ℃ of temperature;
B. the postvaccinal three-spotted phytometra larvae of above-mentioned steps is placed in the case of pupating and pupate, when the body colour for the treatment of pupa after 1-2 days transfers light brown to by milky, move to 3-5 ℃ of temperature and preserve more than 200 days; At this moment, in the three-spotted plusia pupa post the fly larva be in one age initial stage, enter the resting state of surviving the winter with host's pupa;
C. Japan to chase after the best release period of posting fly be pupa time.
The invention has the advantages that: can realize also breeding incessantly in enormous quantities posting fly, be used for the lepidoptera pest in biological control field, thereby reduce the use of chemical pesticide in a large number, reduce residue of pesticide, and use manpower and material resources sparingly.
Embodiment:
Embodiment 1:
Of the present inventionly utilize three-spotted plusia to breed the concrete steps that Japan chases after the method for posting fly for vector:
(1) raises the vector three-spotted plusia
From the healthy larva of field acquisition three-spotted plusia, at 25 ℃ of room temperatures, relative moisture 75%-90%, illumination condition L: D=16: under 8 the condition, raise with the artificial feed that market is bought, larva is gone through phase 10-15 days, 6-9 days pupa time, adult stage 11-15 days;
(2) the fly source breeds
Chased after the three-spotted phytometra larvae of posting the parasitic mistake of fly by Japan from field acquisition, also raise to pupating by the condition of above-mentioned (1) step, near its pupa, gather Japan and chase after the pupa of posting fly, the fly pupa collected put into dependent insect cage, after treating adult eclosion, put into percent by volume in the cage and be 0.5% hydromel and mass percent and be 0.2% milk power solution, supplement the nutrients for adult;
When treating that three-spotted phytometra larvae majority that above-mentioned (1) step is bred was 4 or 5 ages, put it in the inoculation cage, insert after the mating Japan of 6 or 7 days simultaneously and chase after and post the fly adult, be allowed to condition at the larva polypide and go up ovipositing hosts; Fly moth part ratio is 1: 10, and at 25 ℃ of room temperatures, relative moisture 75% or 82% or 90%, illumination condition L: D=16: under 8 the condition, raise with the artificial feed that market is bought, the larva of three-spotted plusia pupated after 1-4 days;
(3) preservation in fly source and release period
A. the fly pupa of collecting by above-mentioned steps was preserved 15 days under 6 ℃ or 7 ℃ of temperature;
B. the postvaccinal three-spotted phytometra larvae of above-mentioned steps is placed in the case of pupating and pupate, when the body colour for the treatment of pupa after 1 or 2 day transfers light brown to by milky, move to 3 ℃ or 4 ℃ or 5 ℃ of temperature and preserve more than 200 days; At this moment, in the three-spotted plusia pupa post the fly larva be in one age initial stage, enter the resting state of surviving the winter with host's pupa;
C. Japan to chase after the best release period of posting fly be pupa time.
Claims (1)
1. one kind is utilized three-spotted plusia to breed Japan for vector to chase after the method for posting fly, it is characterized in that utilizing three-spotted plusia to breed the concrete steps that Japan chases after the method for posting fly for vector:
(1) raises the vector three-spotted plusia
From the healthy larva of field acquisition three-spotted plusia, at 25 ℃ of room temperatures, relative moisture 75%-90%, illumination condition L: D=16: under 8 the condition, raise with the artificial feed that market is bought, larva is gone through phase 10-15 days, 6-9 days pupa time, adult stage 11-15 days;
(2) the fly source breeds
Chased after the three-spotted phytometra larvae of posting the parasitic mistake of fly by Japan from field acquisition, also raise to pupating by the condition of above-mentioned (1) step, in three-spotted plusia pupa time, near its pupa, gather Japan and chase after the pupa of posting fly, the fly pupa collected put into dependent insect cage, after treating adult eclosion, put into percent by volume in the cage and be 0.5% hydromel and mass percent and be 0.2% milk power solution, supplement the nutrients for adult;
Treating that three-spotted phytometra larvae that above-mentioned (1) step breeds is most is 4-5 during age, puts it in the inoculation cage, inserts after the mating Japan of 6-7 days simultaneously and chases after and post the fly adult, is allowed to condition at the larva polypide and goes up ovipositing hosts; Fly moth part ratio is 1: 10, and at 25 ℃ of room temperatures, relative moisture 75%-90%, illumination condition L: D=16: under 8 the condition, raise with the artificial feed that market is bought, the larva of three-spotted plusia pupated after 1-4 days;
(3) preservation in fly source and release period
A. the fly pupa of collecting by above-mentioned steps was preserved 15 days under 6-7 ℃ of temperature;
B. the postvaccinal three-spotted phytometra larvae of above-mentioned steps is placed in the case of pupating and pupate, when the body colour for the treatment of pupa after 1-2 days transfers light brown to by milky, move to 3-5 ℃ of temperature and preserve more than 200 days; At this moment, in the three-spotted plusia pupa post the fly larva be in one age initial stage, enter the resting state of surviving the winter with host's pupa;
C. Japan to chase after the best release period of posting fly be pupa time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011100424537A CN102106324B (en) | 2011-02-22 | 2011-02-22 | Method for breeding exorista japonica townsend by using argyrogramma agnate as intermediate host |
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| CN2011100424537A CN102106324B (en) | 2011-02-22 | 2011-02-22 | Method for breeding exorista japonica townsend by using argyrogramma agnate as intermediate host |
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| CN102106324B CN102106324B (en) | 2012-06-06 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103704070A (en) * | 2013-12-19 | 2014-04-09 | 中国农业科学院草原研究所 | Biological control method for armyworms |
| CN104012474A (en) * | 2014-06-13 | 2014-09-03 | 中国农业科学院植物保护研究所 | Method for judging genetic characteristics of whiteflies in laboratory |
| CN105379685A (en) * | 2015-11-25 | 2016-03-09 | 云南农业大学 | Method for preserving exorista japonica townsend pupae |
| CN111264473A (en) * | 2020-02-18 | 2020-06-12 | 广西壮族自治区林业科学研究院 | Method for breeding Exorista bigelovii by utilizing galleria mellonella |
| CN111345188A (en) * | 2020-03-30 | 2020-06-30 | 海南省农业科学院植物保护研究所 | Spodoptera frugiperda parasitic wasp propagation method |
| CN115176943A (en) * | 2022-06-23 | 2022-10-14 | 苏州大学 | Artificial feed for improving reproductive capacity of Exorista civilis Rond and preparation method thereof |
| CN116114661A (en) * | 2022-12-16 | 2023-05-16 | 青海省农林科学院 | Method for biologically controlling Qinghai grassland caterpillars |
| WO2023245552A1 (en) * | 2022-06-23 | 2023-12-28 | 苏州大学 | Artificial feed for improving reproductive capacity of exorista japonica, and preparation method therefor |
-
2011
- 2011-02-22 CN CN2011100424537A patent/CN102106324B/en not_active Expired - Fee Related
Non-Patent Citations (3)
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| 《昆虫天敌》 19941231 刘军等 银纹夜蛾人工饲养技术研究 170-174 1 第16卷, 第4期 2 * |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704070A (en) * | 2013-12-19 | 2014-04-09 | 中国农业科学院草原研究所 | Biological control method for armyworms |
| CN103704070B (en) * | 2013-12-19 | 2015-09-16 | 中国农业科学院草原研究所 | The biological control method of a kind of mythimna separata |
| CN104012474A (en) * | 2014-06-13 | 2014-09-03 | 中国农业科学院植物保护研究所 | Method for judging genetic characteristics of whiteflies in laboratory |
| CN104012474B (en) * | 2014-06-13 | 2016-01-13 | 中国农业科学院植物保护研究所 | The method of Bemisia tabaci hereditary capacity is judged in a kind of laboratory |
| CN105379685A (en) * | 2015-11-25 | 2016-03-09 | 云南农业大学 | Method for preserving exorista japonica townsend pupae |
| CN105379685B (en) * | 2015-11-25 | 2017-10-10 | 云南农业大学 | A kind of method of the Japanese Exorista civilis pupa of preservation |
| CN111264473A (en) * | 2020-02-18 | 2020-06-12 | 广西壮族自治区林业科学研究院 | Method for breeding Exorista bigelovii by utilizing galleria mellonella |
| CN111345188A (en) * | 2020-03-30 | 2020-06-30 | 海南省农业科学院植物保护研究所 | Spodoptera frugiperda parasitic wasp propagation method |
| CN115176943A (en) * | 2022-06-23 | 2022-10-14 | 苏州大学 | Artificial feed for improving reproductive capacity of Exorista civilis Rond and preparation method thereof |
| CN115176943B (en) * | 2022-06-23 | 2023-11-10 | 苏州大学 | Artificial feed for improving fertility of Japanese chaetoceros and preparation method thereof |
| WO2023245552A1 (en) * | 2022-06-23 | 2023-12-28 | 苏州大学 | Artificial feed for improving reproductive capacity of exorista japonica, and preparation method therefor |
| CN116114661A (en) * | 2022-12-16 | 2023-05-16 | 青海省农林科学院 | Method for biologically controlling Qinghai grassland caterpillars |
| CN116114661B (en) * | 2022-12-16 | 2023-08-29 | 青海省农林科学院 | Method for biologically controlling Qinghai grassland caterpillars |
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| CN102106324B (en) | 2012-06-06 |
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Granted publication date: 20120606 Termination date: 20130222 |