CN115176943A - Artificial feed for improving reproductive capacity of Exorista civilis Rond and preparation method thereof - Google Patents
Artificial feed for improving reproductive capacity of Exorista civilis Rond and preparation method thereof Download PDFInfo
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- CN115176943A CN115176943A CN202210719015.8A CN202210719015A CN115176943A CN 115176943 A CN115176943 A CN 115176943A CN 202210719015 A CN202210719015 A CN 202210719015A CN 115176943 A CN115176943 A CN 115176943A
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- 241001082783 Exorista civilis Species 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 6
- 230000001850 reproductive effect Effects 0.000 title claims description 11
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 claims abstract description 56
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical compound [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 claims abstract description 29
- 229960003638 dopamine Drugs 0.000 claims abstract description 28
- 230000035558 fertility Effects 0.000 claims abstract description 24
- 235000012907 honey Nutrition 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000008223 sterile water Substances 0.000 claims abstract description 16
- 235000013343 vitamin Nutrition 0.000 claims abstract description 13
- 239000011782 vitamin Substances 0.000 claims abstract description 13
- 229940088594 vitamin Drugs 0.000 claims abstract description 13
- 229930003231 vitamin Natural products 0.000 claims abstract description 13
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims abstract description 6
- 235000010234 sodium benzoate Nutrition 0.000 claims abstract description 6
- 239000004299 sodium benzoate Substances 0.000 claims abstract description 6
- 239000007864 aqueous solution Substances 0.000 claims description 19
- 241001124181 Bactrocera dorsalis Species 0.000 claims description 18
- 239000003755 preservative agent Substances 0.000 claims description 13
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 12
- 230000002335 preservative effect Effects 0.000 claims description 11
- 229960003512 nicotinic acid Drugs 0.000 claims description 6
- 235000001968 nicotinic acid Nutrition 0.000 claims description 6
- 239000011664 nicotinic acid Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 5
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 4
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 2
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims description 2
- 239000004288 Sodium dehydroacetate Substances 0.000 claims description 2
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 2
- 229930003270 Vitamin B Natural products 0.000 claims description 2
- 229930003427 Vitamin E Natural products 0.000 claims description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 2
- 229960000304 folic acid Drugs 0.000 claims description 2
- 235000019152 folic acid Nutrition 0.000 claims description 2
- 239000011724 folic acid Substances 0.000 claims description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 2
- 235000010298 natamycin Nutrition 0.000 claims description 2
- 239000004311 natamycin Substances 0.000 claims description 2
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 claims description 2
- 229960003255 natamycin Drugs 0.000 claims description 2
- 235000010241 potassium sorbate Nutrition 0.000 claims description 2
- 239000004302 potassium sorbate Substances 0.000 claims description 2
- 229940069338 potassium sorbate Drugs 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 229960003885 sodium benzoate Drugs 0.000 claims description 2
- 235000019259 sodium dehydroacetate Nutrition 0.000 claims description 2
- 229940079839 sodium dehydroacetate Drugs 0.000 claims description 2
- DSOWAKKSGYUMTF-GZOLSCHFSA-M sodium;(1e)-1-(6-methyl-2,4-dioxopyran-3-ylidene)ethanolate Chemical compound [Na+].C\C([O-])=C1/C(=O)OC(C)=CC1=O DSOWAKKSGYUMTF-GZOLSCHFSA-M 0.000 claims description 2
- 239000011719 vitamin A Substances 0.000 claims description 2
- 235000019155 vitamin A Nutrition 0.000 claims description 2
- 235000019156 vitamin B Nutrition 0.000 claims description 2
- 239000011720 vitamin B Substances 0.000 claims description 2
- 239000011709 vitamin E Substances 0.000 claims description 2
- 235000019165 vitamin E Nutrition 0.000 claims description 2
- 229940046009 vitamin E Drugs 0.000 claims description 2
- 229940045997 vitamin a Drugs 0.000 claims description 2
- 241000184312 Exorista Species 0.000 claims 6
- 235000013601 eggs Nutrition 0.000 abstract description 24
- 241000255925 Diptera Species 0.000 abstract description 19
- 230000017448 oviposition Effects 0.000 abstract description 11
- 241000238631 Hexapoda Species 0.000 abstract description 10
- 230000012447 hatching Effects 0.000 abstract description 9
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 230000001617 migratory effect Effects 0.000 abstract description 3
- 241000255777 Lepidoptera Species 0.000 abstract description 2
- 241001477931 Mythimna unipuncta Species 0.000 abstract description 2
- 241000346285 Ostrinia furnacalis Species 0.000 abstract description 2
- 241000255969 Pieris brassicae Species 0.000 abstract description 2
- 241000012249 Dendrolimus spectabilis Species 0.000 abstract 1
- 241000500441 Plutellidae Species 0.000 abstract 1
- 230000024241 parasitism Effects 0.000 abstract 1
- 241000255789 Bombyx mori Species 0.000 description 12
- 229920000742 Cotton Polymers 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 238000009395 breeding Methods 0.000 description 8
- 241000255985 Trichoplusia Species 0.000 description 7
- 230000001488 breeding effect Effects 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 230000032669 eclosion Effects 0.000 description 6
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 5
- 229940081974 saccharin Drugs 0.000 description 5
- 235000019204 saccharin Nutrition 0.000 description 5
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 5
- 240000000249 Morus alba Species 0.000 description 4
- 235000008708 Morus alba Nutrition 0.000 description 4
- 230000000384 rearing effect Effects 0.000 description 4
- 241001490361 Bactrocera minax Species 0.000 description 3
- 244000184734 Pyrus japonica Species 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000008187 granular material Substances 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000035479 physiological effects, processes and functions Effects 0.000 description 3
- 241000191839 Chrysomya Species 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000019617 pupation Effects 0.000 description 2
- 241001290610 Abildgaardia Species 0.000 description 1
- 241001491790 Bupalus piniaria Species 0.000 description 1
- 241001316290 Gypsophila Species 0.000 description 1
- 241001531327 Hyphantria cunea Species 0.000 description 1
- 241000283956 Manis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000257159 Musca domestica Species 0.000 description 1
- 241000406583 Parasitidae Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241001124066 Tachinidae Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
- A23K20/126—Lactones
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Inorganic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses an artificial feed for improving the fertility of Exorista civilis Rond and a preparation method thereof, wherein the artificial feed comprises the following components: sterile water, honey, vitamins, sodium benzoate, nano titanium dioxide and dopamine. The Japanese fly larvae are natural enemy insects for biological control, and lepidoptera pests such as diamondback moths, pine caterpillars, armyworms, cabbage butterflies, corn borers and the like can be parasitized by adult eggs and are widely applied to biological control. The life span and egg-producing ability of adults are key factors affecting the parasitism ability of the Japan migratory flies. The artificial feed has the characteristics of long quality guarantee period and high feed utilization rate. The feed can obviously prolong the service life of the adult Chrysomyia Nucifera, improve the egg laying amount and the egg hatching rate of the Chrysomyia Nucifera, and is suitable for popularization in the raising of the Chrysomyia Nucifera.
Description
Technical Field
The invention belongs to the field of biological control, relates to a breeding method of natural enemy insects, and particularly relates to an artificial feed for improving the fertility of Japan migratory flies.
Background
Gypsophila japonica (Misch.) KuckExorista japonica(Townsend) (Diptera parasitidae) are large-egg type parasitic flies, which lay eggs on the body surface of a host, hatch and then bore into the body cavity of the host, and finally bore out the host pupation at the host larva or pupation stage. It can parasitize various agricultural and forestry pests, including 30 kinds of lepidoptera pests such as fall webworm, pine moth, armyworm, cabbage butterfly, corn borer, etc. The biological control of the pests through the parasitoid fly reduces the pesticide pollution, protects the environment and ensures the quality and quantity of agriculture, forestry and animal husbandry production.
The breeding technology of the larvae of the bactrocera dorsalis is more researched, the development of the adult feed of the bactrocera dorsalis is less reported, and CN102106324A utilizes the Trichoplusia agnata as an intermediate host to breed the Trichoplusia japonica indoors, utilizes artificial feed to breed the Trichoplusia agnata indoors, utilizes the Trichoplusia agnata as an intermediate host to breed the Trichoplusia agnata indoors, and utilizes the Trichoplusia agnata as the storage and release period of the pupa of the Trichoplusia manis. The feed adopted in the prior production is a mixture of honey water solution or honey and saccharin, and the existing feed is used for feeding the adult bactrocera dorsalis, so that the service life is short and is less than 18 days; the fertilization rate of the offspring eggs is lower, the hatching rate is lower than 50%, and the breeding work of the Exorista civilis Rond is adversely affected. At present, no research report for improving the reproductive capacity of the bactrocera dorsalis exists. Therefore, the development of efficient artificial feed for imagoes is urgently needed in the breeding of the bactrocera dorsalis, the hatching rate of eggs is improved, and the application of the bactrocera dorsalis in biological control is strengthened.
Disclosure of Invention
The invention aims to provide an artificial feed formula for improving the fertility of the Exorista civilis Rond.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
an artificial feed for improving the fertility of the Exorista civilis Rond comprises sterile water, honey, vitamins, preservatives, nano titanium dioxide and dopamine. Preferably, the artificial feed for improving the reproductive capacity of the Exorista civilis Rond consists of sterile water, honey, vitamins, a preservative, nano titanium dioxide and dopamine.
Furthermore, the consumption of the honey is 3-8 per mill of the total weight of the artificial feed for improving the reproductive capacity of the Chrysomya diplodis Hemsl.
In the invention, the vitamins comprise one or more of vitamin A, vitamin E, vitamin B, folic acid and nicotinic acid, and the dosage of the vitamins is 0.2 to 0.5 per thousand of the total weight of the artificial feed for improving the fertility of the Japan house fly.
In the invention, the preservative comprises one or more of sodium benzoate, potassium sorbate, sodium dehydroacetate and natamycin, and the dosage of the preservative is 0.3 to 0.6 per mill of the total weight of the artificial feed for improving the fertility of the Japan bactrocera dorsalis.
In the invention, the nano titanium dioxide exists in the form of nano titanium dioxide aqueous solution, and the particle size of the nano titanium dioxide is 5 to 15 nm; the addition amount of the nano titanium dioxide aqueous solution is 0.1 to 0.35 per mill of the total volume of the artificial feed for improving the reproductive capacity of the Chrysomya diplodis Hemsl; the dopamine exists in dopamine aqueous solution, and the addition amount of the dopamine aqueous solution is 0.03 to 0.055 thousandth of the total volume of the artificial feed for improving the reproductive capacity of the Exorista civilis Rond. Furthermore, the concentration of the nano titanium dioxide aqueous solution is 3 to 5mg/L, and the concentration of the dopamine aqueous solution is 1 to 3 mg/L.
The preparation method of the artificial feed for improving the reproductive capacity of the Exorista civilis Rond comprises the following steps:
(1) Adding nano titanium dioxide into sterile water to obtain a nano titanium dioxide aqueous solution;
(2) Dissolving dopamine powder in sterile water to obtain a dopamine aqueous solution;
(3) Mixing honey, vitamins, a preservative, sterile water, a nano titanium dioxide aqueous solution and a dopamine aqueous solution, and then sterilizing at high temperature and high pressure to obtain the artificial feed.
According to the invention, the nano titanium dioxide and the dopamine are selectively added, so that the method has obvious advantages in the aspects of prolonging the service life of the imagoes of the Exorista civilis Rond and improving the fertility, and is provided for the first time.
According to the invention, the vitamins, the nano titanium dioxide, the preservative and the dopamine are added into the artificial feed, so that the service life of the imagoes of the Exorista civilis Rond is prolonged, the egg laying amount and the offspring hatching rate of the Exorista civilis Rond are improved, and the effect is obvious. Meanwhile, the artificial feed prepared by the invention has long quality guarantee period during feeding of the bactrocera dorsalis and is not easy to decay, so that the artificial feed is worthy of being popularized and applied in the bactrocera dorsalis nursing work.
Detailed Description
The following is a further detailed description of specific embodiments of the invention.
Test materials: nicotinic acid, sodium benzoate, nano titanium dioxide and dopamine are purchased from biological engineering (Shanghai) GmbH; the honey is a second-grade honey according to the requirements of national honey quality standard of GH/T18796-2012 honey; the honey is a conventional feed product.
The Japan Pietnam sedge is a resource preserved by the mulberry institute of Suzhou university.
Example one
An artificial feed for improving the fertility of the Exorista civilis Rond comprises the following components: 995 mL of sterile water, 5g of honey, 0.3 g of nicotinic acid, 0.5 g of sodium benzoate, 300 mu L of nano titanium dioxide solution and 50 mu L of dopamine solution.
The preparation method comprises the following steps:
step 1, preparing a nano titanium dioxide solution: adding nano titanium dioxide (D90 with the particle size of 5 nm) into sterile water, and performing conventional ultrasonic treatment to prepare nano titanium dioxide aqueous dispersion with the concentration of 3 mg/L;
step 2, preparing a dopamine solution: dissolving solid dopamine in sterile water, and uniformly shaking by conventional vortex; preparing a dopamine aqueous solution with the concentration of 1mg/L;
step 3, weighing 5g of honey, 0.3 g of nicotinic acid and 0.5 g of sodium benzoate by using a balance, taking 995 mL of water by using a measuring cylinder, and taking 300 mu L of titanium dioxide solution and 50 mu L of dopamine solution by using a pipettor; adding all the components into a 1L reagent bottle, and sterilizing at 120 deg.C under high pressure of 0.12 MPa for 15 min to obtain the artificial feed for improving the fertility of imago of Chrysomyia macrantha.
And (4) feeding the imagoes of the Chrysomyia diplodis. The artificial feed prepared by the method is used for feeding the imagoes of the bactrocera dorsalis. The imagoes of the bactrocera dorsalis are eclosion in the same batch, and no obvious difference in physiology and the like of the imagoes is ensured. A pair of male and female flies are placed in the same miniature insect breeding cage, 100 cages are bred together, the breeding environment is 16 h of illumination/8 h of darkness, the temperature is 25 +/-1 ℃, and the relative humidity is 75 +/-5%. Feed (10 ml) was soaked cotton (2 g), cotton was spread into petri dishes (diameter 60 mm), and feed soaked cotton was replaced once a week. After the imagoes of the fly hunting flies eclosion for 5 days, 20 silkworms of 1 day of age 5 are placed in an insect rearing cage for the imagoes of the fly hunting flies to lay eggs, the silkworms are taken out after 10 min of egg laying, and the silkworms are bred by mulberry leaves until the silkworms cluster and form cocoons.
And (5) counting the service life and the egg hatching rate of the imagoes of the bactrocera dorsalis. The time from emergence to death (longevity), the amount of eggs laid and the hatchability of the eggs were recorded for the flies from the top under the feed conditions (see table 1).
The average life (days) of the feed-raised imagoes of the bactrocera dorsalis hendel in this example: for 20 days. Average egg laying amount of single female flies (within 10 min): 6 granules. Average egg hatchability: 85 percent.
Example two
The D90 particle size of the nano titanium dioxide is adjusted from 5 nm to 15 nm, and the average service life (days) of the imagoes of the Exorista civilis Rond is as follows: 20.5 days; average egg laying amount of single female flies (within 10 min): 7 grains; average egg hatchability: 82 percent.
The concentration of the nano titanium dioxide solution is adjusted to be 5mg/L, and the average life (days) of the imagoes of the Exorista civilis Rond is as the same as that of the first embodiment: 22 days; average egg laying amount of single female flies (within 10 min): 10 grains; average hatching rate of eggs: 84 percent.
The dopamine solution concentration was adjusted to 3mg/L, and the average life (days) of the imagoes of the fly catch flies was as in example one: 22 days; average egg laying amount of single female flies (within 10 min): 9 grains of the mixture; average egg hatchability: 89 percent.
Comparative example 1
Diluting 5g Mel and 0.3 g nicotinic acid in 995 mL sterile water, sterilizing at 120 deg.C and 0.12 MPa for 15 min, and refrigerating at 4 + -1 deg.C.
The adult insects of the Chrysomyia diplodis (Ex. Fr.) Sing were bred in parallel with the first example. The imagoes of the bactrocera dorsalis are eclosion in the same batch, so that no obvious difference in physiology and the like of the imagoes is ensured. A pair of male and female flies are placed in the same miniature insect breeding cage, 100 cages are bred together, the breeding environment is 16 h of illumination/8 h of darkness, the temperature is 25 +/-1 ℃, and the relative humidity is 75 +/-5%. Cotton (2 g) was soaked in feed (10 mL), the cotton was spread in petri dishes (60 mm diameter), and the cotton with feed added once was changed for three days (since no preservative was added, it was easily rotten). After the imagoes of the fly hunting flies eclosion for 5 days, 20 silkworms of 1 day of age 5 are placed in an insect rearing cage for the imagoes of the fly hunting flies to lay eggs, the silkworms are taken out after 10 min of egg laying, and the silkworms are bred by mulberry leaves until the silkworms cluster and form cocoons.
The time from emergence to death (longevity), the amount of eggs laid, and the hatching rate of the eggs were recorded for the chasing flies under the feed conditions (see table 2).
The average service life (days) of the imagoes of the bactrocera dorsalis fed by the feed is as follows: and (5) 17 days. Average egg laying amount of single female flies (within 10 min): 2 granules. Average egg hatchability: 50 percent.
Comparative example No. two
Adding 10 g of Mel and 0.2 g of saccharin into 1L of sterile water, sterilizing at 120 deg.C under high pressure of 0.12 MPa for 15 min to obtain feed containing Mel and saccharin mixture, which is conventional feed for existing production.
The adult insects of the Chrysomyia diplodis (Ex. Fr.) Sing were bred in parallel with the first example. The imagoes of the bactrocera dorsalis are eclosion in the same batch, so as to ensure that the imagoes have no obvious difference in the aspects of physiology and the like. A pair of male and female flies are placed in the same miniature insect rearing cage, and are reared in 100 cages together under the condition of 16 h illumination/8 h darkness, the temperature is 25 +/-1 ℃, and the relative humidity is 75 +/-5%. Cotton (2 g) was soaked in feed (10 mL), the cotton was spread in petri dishes (60 mm diameter), and the cotton with feed added once was changed for three days (since no preservative was added, it was easily rotten). After the imagoes of the fly hunting flies eclosion for 5 days, 20 silkworms of 1 day of age 5 are placed in an insect rearing cage for the imagoes of the fly hunting flies to lay eggs, the silkworms are taken out after 10 min of egg laying, and the silkworms are bred by mulberry leaves until the silkworms cluster and form cocoons.
The time from emergence to death (longevity), the amount of eggs laid, and the hatching rate of the eggs were recorded for the chasing flies under the feed conditions (see table 3).
The average service life (days) of the imagoes of the bactrocera dorsalis fed by the feed is as follows: and 15 days. Average egg laying amount of single female flies (within 10 min): 3 granules of the composition. Average egg hatchability: 42 percent.
The results show that the mixture of the honey and the saccharin, and the mixture of the honey and the vitamins are used as the feed, the service life of the imagoes of the migratory flies and the development condition of offspring are not good, and the effect is worse when the honey water is used as the feed alone.
On the basis of the first embodiment, dopamine is omitted, and the average egg hatching rate of the obtained feed is 58%; on the basis of the first embodiment, the nano titanium dioxide is omitted, and the average egg hatchability of the obtained feed is 72%.
In a word, compared with the common feed of honey water and a mixture of honey and saccharin, the feed for feeding the adult bactrocera minax can obviously prolong the service life of the adult bactrocera minax, improve the fertility and the offspring hatchability and provide a foundation for the mass propagation of the adult bactrocera minax.
Claims (10)
1. An artificial feed for improving the fertility of the Exorista civilis Rond is characterized by comprising sterile water, honey, vitamins, a preservative, nano titanium dioxide and dopamine.
2. The artificial feed for improving the fertility of the Exorista civilis Rond of claim 1, wherein the honey is added in an amount of 3 to 8% by weight based on the total weight of the artificial feed for improving the fertility of the Exorista civilis Rond.
3. The artificial feed for improving the fertility of the Exorista civilis Rond according to claim 1, wherein the vitamins include one or more of vitamin A, vitamin E, vitamin B, folic acid and nicotinic acid, and the addition amount of the vitamins is 0.2 to 0.5 per mill of the total weight of the artificial feed for improving the fertility of the Exorista civilis Rond.
4. The artificial feed for improving the fertility of the Exorista civilis Rond of claim 1, wherein the preservative comprises one or more of sodium benzoate, potassium sorbate, sodium dehydroacetate and natamycin, and the addition amount of the preservative is 0.3 to 0.6 per mill of the total weight of the artificial feed for improving the fertility of the Exorista civilis Rond.
5. The artificial feed for improving the reproductive capacity of Exorista nipponensis according to claim 1, wherein the nano titanium dioxide is present in the form of a nano titanium dioxide aqueous solution; the addition amount of the nano titanium dioxide aqueous solution is 0.1 to 0.35 per thousand of the total volume of the artificial feed for improving the reproductive capacity of the Japan bactrocera dorsalis.
6. The artificial feed for improving the fertility of Exorista nipponensis according to claim 1, wherein dopamine is present in an aqueous solution of dopamine; the addition amount of the dopamine aqueous solution is 0.03 to 0.055 per mill of the total volume of the artificial feed for improving the reproductive capacity of the Exorista civilis Rond.
7. The method for preparing an artificial feed for improving the fertility of Exorista civilis Rond according to claim 1, wherein the artificial feed for improving the fertility of Exorista civilis Rond is obtained by mixing sterile water, honey, vitamins, preservatives, nano titanium dioxide and dopamine.
8. The method for preparing the artificial feed for improving the fertility of the Exorista civilis Hance according to claim 7, comprising the steps of: (1) Adding nano titanium dioxide into sterile water to obtain a nano titanium dioxide aqueous solution; (2) Dissolving dopamine powder in sterile water to obtain a dopamine aqueous solution; (3) Mixing honey, vitamins, a preservative, sterile water, a nano titanium dioxide aqueous solution and a dopamine aqueous solution, and then sterilizing at high temperature and high pressure to obtain the artificial feed.
9. Use of the artificial feed for improving the fertility of Exorista nipponensis according to claim 1 for feeding Exorista nipponensis.
10. Use of the artificial feed for improving the fertility of Exorista nipponensis according to claim 1 for the preparation of a feed for improving the fertility of Exorista nipponensis.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116114661A (en) * | 2022-12-16 | 2023-05-16 | 青海省农林科学院 | Method for biologically controlling Qinghai grassland caterpillars |
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| CN102106324A (en) * | 2011-02-22 | 2011-06-29 | 云南农业大学 | Method for breeding exorista japonica townsend by using argyrogramma agnate as intermediate host |
| CN103947614A (en) * | 2014-04-24 | 2014-07-30 | 苏州大学 | Method for inhibiting in-vivo BmNPV viral multiplication of silkworms |
| CN110432407A (en) * | 2019-08-28 | 2019-11-12 | 苏州大学 | A kind of functionality silkworm artificial feed and the preparation method and application thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106324A (en) * | 2011-02-22 | 2011-06-29 | 云南农业大学 | Method for breeding exorista japonica townsend by using argyrogramma agnate as intermediate host |
| CN103947614A (en) * | 2014-04-24 | 2014-07-30 | 苏州大学 | Method for inhibiting in-vivo BmNPV viral multiplication of silkworms |
| CN110432407A (en) * | 2019-08-28 | 2019-11-12 | 苏州大学 | A kind of functionality silkworm artificial feed and the preparation method and application thereof |
| WO2021037180A1 (en) * | 2019-08-28 | 2021-03-04 | 苏州大学 | Functional artificial feed for silkworms, preparation method and application thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116114661A (en) * | 2022-12-16 | 2023-05-16 | 青海省农林科学院 | Method for biologically controlling Qinghai grassland caterpillars |
| CN116114661B (en) * | 2022-12-16 | 2023-08-29 | 青海省农林科学院 | Method for biologically controlling Qinghai grassland caterpillars |
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