CN113331087A - Artificial propagation method of Gymnocypris ventricosa - Google Patents
Artificial propagation method of Gymnocypris ventricosa Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention provides an artificial propagation method of Gymnothorax biddulphi, which comprises the following steps: parent fish breeding: selecting the wild aphyllobelia affinis with no damage to the body surface and the weight of 400-1300g as parent fishes for breeding, wherein the daily feeding amount of the feed in the breeding process is 0.1-1% of the weight of the parent fishes; selecting parent fish: selecting mature Gymnothorax biddulphi with no disease and strong constitution as an induced spawning parent; artificial hastening parturition: firstly, injecting a first oxytocic compatibility only for female fish in an oxytocic parent; after 24 hours, continuing to inject a second oxytocic for compatibility with the female fish, and injecting a third oxytocic for compatibility with the male fish in the oxytocic parent; artificial insemination: temporarily breeding the spawning induction parent in a spawning induction pool at the temperature of 9-14 ℃, and checking female fish in the spawning induction parent every 8 hours after 48 hours; slightly pressing the abdomen of the female fish, squeezing the roe into a basin if the roe is discharged, squeezing the semen of the male fish into the basin, stirring, standing and cleaning to obtain fertilized eggs; hatching: and (4) hatching the fertilized eggs obtained by using running water to obtain the larval fish of the Gymnocypris ventricosa.
Description
Technical Field
The invention belongs to the technical field of fish breeding, and particularly relates to an artificial breeding method of Gymnocypris.
Background
Naked ventral beard fish, school name: ptychobarbus kaznakvi Nikolsky, a fish of genus Leptospira of family Cyprinidae of order Cyprinales, commonly known as flower fish, is a unique species in China. The Gymnothorax kidako is distributed in the water system of Jinshajiang river, billu river, the dry branch of the anger river and the like, and generally lives in the running environment of the dry branch and the flowing water. In recent years, due to high-strength fishing, the fishing amount far exceeds the natural proliferation amount, the change of ecological environment, water pollution and the like, the population quantity is sharply reduced. In some sections of the river, there have been substantial absences with only a few individuals in remote areas upstream. The endangered grade is easy to endanger, so that the artificial propagation method suitable for the aphelenchoides besseyi is particularly important to provide.
Research on Gymnocypris ventricosa has been carried out, and some scholars firstly carried out classification and distribution research, such as Wuyunfei, Wucuizhen (Tibet plateau fish, Sichuan scientific and technical publisher, 1991.415-419), Tibet autonomous region aquatic agency (Tibet fish and its resources, Chinese agricultural publisher, 1995.103-109); some scholars have studied the age and growth of the bare belly beard fish, such as li fei, yang germany, heyofeng, yao deng, yao jia wei, julian (giving the age and growth of the bare belly beard fish, freshwater fishery, 2016 (06))), li lobster, zhuangye, liuming classic, wang, liushao ping, and chun (age structure and growth characteristics of the bare belly beard fish upstream of anger, freshwater fishery, 2019 (04)); liyanpeng studied the biological properties and protection of aphelenchoides (biological properties and protection recommendation for aphelenchoides, fishery of north river, 2016 (03)); the food habits of the gymnasium partnervis are studied by the young soldiers such as the hippeastrum, the plum flies and the aspen germany (the food habits of the fishes which are provided with the yeast upstream of the Jinshajiang river and the gymnasium partnervis, the Yangtze river basin resources and the environment, 2016(07)), but no relevant research is carried out on other aspects of the gymnasium partnervis, particularly on the aspect of artificial propagation technology, and the specifications and the protection of the artificial propagation of the gymnasium partnervis are still in the blank stage in China.
Disclosure of Invention
In view of the above, the present invention provides an artificial propagation method suitable for aphelenchoides besseyi.
The invention provides an artificial propagation method of Gymnocypris ventricosa, which comprises the following steps:
step S101, parent fish breeding: selecting the wild aphyllobelia affinis with no damage to the body surface and the weight of 400-1300g as parent fishes for breeding, wherein the daily feeding amount of the feed in the breeding process is 0.1-1% of the weight of the parent fishes;
step S102, parent fish selection: selecting mature Gymnothorax biddulphi with no disease and strong constitution as an induced spawning parent;
step S103, artificial induced spawning: firstly, injecting a first oxytocic compatibility only for female fish in an oxytocic parent; and after 24 hours, continuing to inject a second oxytocic for compatibility to female fish, and injecting a third oxytocic for compatibility to male fish in the oxytocic parents. Wherein the first oxytocin formulation comprises a luteinizing hormone releasing hormone analogue with a dosage of 3-8 mug/kg and a dehydrogenine maleate with a dosage of 2-6mg/kg, the second oxytocin formulation comprises a luteinizing hormone releasing hormone analogue with a dosage of 10-12 mug/kg, a dehydrogenine maleate with a dosage of 6-10mg/kg and a chorionic gonadotropin with a dosage of 500-1000 international units/kg, and the third oxytocin formulation comprises a luteinizing hormone releasing hormone analogue with a dosage of 5-6 mug/kg, a dehydrogenine maleate with a dosage of 3-5mg/kg and a chorionic gonadotropin with a dosage of 250-500 international units/kg;
step S104, artificial insemination: temporarily breeding induced spawning parents in an induced spawning pond at the water temperature of 9-14 ℃, checking female fish in the induced spawning parents every 8 hours after 48 hours, slightly pressing the belly of the female fish, squeezing fish eggs into a basin if fish eggs are produced, squeezing sperm of male fish into the basin, stirring, standing and cleaning to obtain fertilized eggs;
step S105, hatching: and (5) hatching the fertilized eggs obtained in the step S104 by using running water to obtain the larval fish of the Gymnocypris ventricosa.
Further, in step S101, the parent fish is cultured at a density of 3.5kg/m3The pH value of the water for culturing the parent fish is 7.6-8.4, the water flow is 15-20L/min, the water dissolved oxygen is 6-10mg/L, and the water temperature is 4-17 ℃.
Further, in step S101, the protein content of the feed used in the cultivation process is 36-41%, and the feed is fed 2 times a day at 9 am and 6 pm, respectively.
Further, in step S101, water earthworms are added to increase protein intake during parent fish breeding, and the daily feeding amount of the water earthworms is 0.1-0.8% of the weight of the parent fish.
Further, in step S102, the selected mature male and female bare belly beard fish is placed in an induced spawning pond, the ratio of male and female parent fish is 1:1, 2-10 pairs of parent fish are selected each time, and the placement density is less than 5kg/m3The water temperature in the spawning pool is 9-14 ℃, the dissolved oxygen of the water body is 6-10mg/L, and the pH value of the water is 7.8-8.2.
Further, in step S105, the length and width of the hatching frame used in the hatching process is 45 cm to 35cm, and at most 3000 + 5000 fertilized eggs are put into each hatching frame without overlapping; the water temperature during incubation is 9-12 deg.C, incubation time is 14-17 days, fertilized eggs can not roll during incubation, water flow is 4.5-6L/min, water dissolved oxygen is 6-10mg/L, and water pH is 7.8-8.2.
The technical scheme provided by the invention has the beneficial effects that: the artificial propagation method provided by the invention is simple to operate, can provide a large number of seedlings for the mass production of the aphelenchoides nudus, realizes the proliferation and releasing of the aphelenchoides nudus, effectively protects ecological resources, and solves the problem that the number of the aphelenchoides nudus is gradually reduced; the experiment shows that the method of the invention has the spawning induction rate of 75-100 percent, the fertilization rate of 92.42-94.67 percent and the hatchability of 80.97-91.83 percent.
Drawings
FIG. 1 is a schematic flow chart of an artificial propagation method of Gymnocypris.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be further described with reference to the accompanying drawings.
Referring to fig. 1, an embodiment of the present invention provides an artificial propagation method of aphelenchoides pteronyssinus, including the following steps:
step S101, parent fish breeding: selecting wild Gymnothorax parviflora with no damage to body surface and weight of 400-1300g as parent fish for breeding, placing the selected parent fish into a round glass jar, and breeding with a circulating water breeding system, wherein the breeding density is 3.5kg/m3The bacillus subtilis is added into the circulating water to control the water quality, the pH value of the water is 7.6-8.4, the water flow is 15-20L/min, the water dissolved oxygen is 6-10mg/L, the water temperature is 4-17 ℃, and the bacillus subtilis is cultured dailyThe feed used in the process is a compound feed for special marine fish with the protein content of 36-41%, the daily feeding amount of the feed is 0.1-1% of the weight of parent fish, and the feed is fed for 2 times every day at 9 am and 6 pm respectively; the feed can also be added with water earthworms to increase the intake of animal protein so as to strengthen the cultivation, and the daily feeding amount of the water earthworms is 0.1-0.8 percent of the weight of parent fishes;
step S102, parent fish selection: selecting disease-free and strong mature Gymnothorax przewalskii from a recirculating aquaculture system in 5 months as an induced spawning parent, putting the mature Gymnothorax przewalskii into a circular induced spawning pond, and placing the mature Gymnothorax przewalskii with the density of less than 5kg/m3The water flow in the spawning pool is 4.5-6L/min, the water temperature in the spawning pool is controlled at 9-14 ℃, the dissolved oxygen of the water body is 6-10mg/L, and the pH value of the water is 7.8-8.2;
step S103, artificial induced spawning: firstly, injecting a first oxytocic compatibility only for female fish in an oxytocic parent; after 24 hours, continuing to inject a second oxytocic for compatibility with the female fish, and simultaneously injecting a third oxytocic for compatibility with the male fish in the oxytocic parent; wherein the first oxytocin formulation comprises a luteinizing hormone releasing hormone analogue with a dosage of 3-8 mug/kg and a dehydrogenine maleate with a dosage of 2-6mg/kg, the second oxytocin formulation comprises a luteinizing hormone releasing hormone analogue with a dosage of 10-12 mug/kg, a dehydrogenine maleate with a dosage of 6-10mg/kg and a chorionic gonadotropin with a dosage of 500 international units/kg, and the third oxytocin formulation comprises a luteinizing hormone releasing hormone analogue with a dosage of 5-6 mug/kg, a dehydrogenine maleate with a dosage of 3-5mg/kg and a chorionic gonadotropin with a dosage of 250 international units/kg;
step S104, artificial insemination: after 48 hours, checking the development condition of female fish in the induced spawning parents once every 8 hours, if the belly of the female fish is lightly pressed and fish eggs are discharged, lifting the female fish, wrapping the female fish with a towel, wiping off body surface water, extruding the fish eggs into a basin, lifting the male fish, wrapping the male fish with a towel, wiping off body surface water, extruding seminal fluid into the basin, lightly stirring the seminal fluid for 1 minute by using feathers, adding clear water, standing for 2 minutes, washing the eggs for 4-5 times by using the clear water, and washing off redundant seminal fluid to obtain fertilized eggs;
step S105, hatching: placing fertilized eggs in an incubation frame in a circulating water culture system, incubating by using running water, wherein the length and the width of the incubation frame are 45 and 35cm, 3000 plus 5000 fertilized eggs are put in each frame at most, and the fertilized eggs are not overlapped; the water temperature during incubation is 9-12 deg.C, incubation time is 14-17 days, water flow is 4.5-6L/min, water dissolved oxygen is 6-10mg/L, and pH value of water is 7.8-8.2. Keeping the fertilized eggs in a static state in an incubation frame, cleaning dead eggs in time in the incubation process, monitoring and recording the information of good water temperature, water quality and the like, and obtaining the larval fish of the nude ventral lobe beard after the incubation is finished.
The method for artificially breeding aphyllophagus fasciatus according to the present invention will be described in detail with reference to the following examples.
Example 1:
selecting wild Gymnothorax parviflora with body surface injury free and weight of 400-900g as parent fish for breeding, placing the selected parent fish into a round glass jar, and breeding with a circulating water breeding system, wherein the breeding density is 3.5kg/m3Adding bacillus subtilis into circulating water to control water quality, wherein the water dissolved oxygen is 7mg/L, the pH value of water is 8.0, the water flow is 18L/min, the water temperature is 4-15 ℃, the feed used in the cultivation process is a special marine fish compound feed with the protein content of 36%, the daily feeding amount is 0.5% of the weight of parent fish, and the feeding is respectively carried out for 1 time at 9 am and 6 pm each day; selecting 2 pairs of disease-free and strong mature Gymnothorax przewalskii from a recirculating aquaculture system in 5 months as spawning-inducing parents, placing the mature Gymnothorax przewalskii in a circular spawning-inducing pond with the placement density of 3kg/m3The water flow is 5L/min, the water temperature in the spawning pool is controlled at 9 ℃, the dissolved oxygen of the water body is 8mg/L, and the pH value of the water is 8.0; firstly, injecting a first oxytocic compatibility (the components are 3 mug/kg of luteinizing hormone releasing hormone analogue and 4mg/kg of diutanone maleate) only to female fish in an oxytocic parent; injecting second oxytocin for 24h after injection (composed of lutein release hormone analogue with dosage of 11 μ g/kg, diutanone maleate with dosage of 6mg/kg and chorionic gonadotropin with dosage of 500 international units/kg); injecting third oxytocic into male fish of oxytocic parent (composed of 5 μ g/kg lutein-releasing hormone analogue, 3mg/kg diutanone maleate and 250 mg/kg)Chorionic gonadotropin per kg of intersomatic units); checking the ovulation condition of the parent fish after 56 hours, slightly pressing the belly of the female fish to discharge fish eggs, lifting the female fish, wrapping the female fish with a towel, wiping off body surface moisture, squeezing the fish eggs into a basin, lifting the male fish, wrapping the male fish with a towel, wiping off the body surface moisture, squeezing semen into the basin, slightly stirring the semen with feathers for 1 minute, adding clear water, standing for 2 minutes, washing the eggs with clear water for 4 times, and washing off redundant semen to obtain fertilized eggs; putting fertilized eggs into an incubation frame in a circulating water culture system, and incubating by using running water, wherein the length and the width of the incubation frame are 45 and 35cm, 3000 fertilized eggs are put into each frame, and the eggs are not overlapped; the flow rate of water is 5L/min, the dissolved oxygen of water is 8mg/L, the pH value of the water is 8.0, the incubation water temperature is 10 ℃, the incubation time is 16 days, fertilized eggs are kept in a static state in an incubation frame, dead eggs are cleaned in time in the incubation process, the information of good water temperature, good water quality and the like is monitored and recorded, and the larval fish of the Gymnothorax parva is obtained after the incubation is finished.
In example 1, the spawning induction rate of the aphelenchoides farinae is 75%, the fertilization rate is 92.42%, and the hatchability rate is 80.97%.
Example 2:
selecting wild Gymnothorax parviflora with no damage to body surface and weight of 700-1300g as parent fish for breeding, placing the selected parent fish into a round glass jar, and breeding with a circulating water breeding system, wherein the breeding density is 3.5kg/m3Adding bacillus subtilis into circulating water to control water quality, wherein the pH value of the water is 7.8, the water flow is 16L/min, the dissolved oxygen amount of the water body is 7mg/L, the water temperature is 13 ℃, the feed used in the daily cultivation process is a special marine fish compound feed with the protein content of 41 percent, the daily feeding amount is 0.8 percent of the weight of parent fish, feeding is respectively carried out for 1 time every day at 9 am and 6 pm, and tubificidae with the weight of 0.2 percent of the weight of the parent fish is fed every day in the later cultivation process; selecting 10 pairs of disease-free and strong mature Gymnothorax przewalskii from a recirculating aquaculture system in 5 months as spawning-inducing parents, putting the mature Gymnothorax przewalskii into a circular spawning-inducing pond, and placing the mature Gymnothorax przewalskii at a density of 4kg/m3The water flow is 5.5L/min, the water temperature in the spawning pool is controlled at 10 ℃, the dissolved oxygen of the water body is 7mg/L, and the pH value of the water is 8.0; firstly, only the female fish in the induced spawning parent is injectedFirst oxytocin compatibility (composition comprises luteinizing hormone releasing hormone analogue with dosage of 8 μ g/kg and diutanone maleate with dosage of 2 mg/kg); continuing to inject a second oxytocin formulation (consisting of lutein release hormone analog with a dose of 10 mug/kg, diutanone maleate with a dose of 8mg/kg and chorionic gonadotropin with a dose of 700 IUU/kg) into the female fish 24h after the injection is finished; injecting a third oxytocin compatibility (the components are lutein releasing hormone analogue with the dosage of 6 mug/kg, diutanone maleate with the dosage of 3.5mg/kg and chorionic gonadotropin with the dosage of 400 international units/kg) to the male fish in the oxytocin parent; checking the ovulation condition of the parent fish after 48 hours, slightly pressing the belly of the female fish to discharge fish eggs, lifting the female fish, wrapping the female fish with a towel, wiping off body surface moisture, squeezing the fish eggs into a basin, lifting the male fish, wrapping the male fish with a towel, wiping off the body surface moisture, squeezing semen into the basin, slightly stirring the semen with feathers for 1 minute, adding clear water, standing for 2 minutes, washing the eggs with clear water for 4 times, and washing off redundant semen to obtain fertilized eggs; putting fertilized eggs into an incubation frame in a circulating water culture system, and incubating by using running water, wherein the length and the width of the incubation frame are 45 and 35cm, 5000 fertilized eggs are put into each frame, and the fertilized eggs are not overlapped; the water flow is 6L/min, the water dissolved oxygen is 10mg/L, the pH value of the water is 8.2, and the water temperature is 12 ℃. Keeping the fertilized eggs in a static state in an incubation frame, cleaning dead eggs in time in the incubation process, monitoring and recording the information of good water temperature, water quality and the like, and incubating for 14 days to obtain the larval fish of the Gymnocypris ventricosa.
In example 2, the spawning induction rate of the aphelenchoides farinae is 100%, the fertilization rate is 94.2%, and the hatchability is 85.7%.
Example 3:
selecting wild Gymnothorax davidi with no damage to body surface and weight of 600-1100g as parent fish for breeding, placing the selected parent fish into a round glass jar, and breeding with a circulating water breeding system, wherein the breeding density is 3.5kg/m3The bacillus subtilis is added into circulating water to control water quality, the pH value of the water is 8.0, the water flow is 17L/min, the water temperature is 16 ℃, the water dissolved oxygen is 9mg/L, the feed used in the daily cultivation process is the compound feed for the special marine fish with the protein content of 40 percent, and the daily feeding amount is parentFeeding 0.6% of fish weight for 1 time at 9 am and 6 pm respectively, and feeding tubificidae with parent fish weight of 0.4% in late stage culture process; selecting 4 pairs of disease-free and strong mature Gymnothorax przewalskii from a recirculating aquaculture system in 5 months as spawning-inducing parents, putting the mature Gymnothorax przewalskii into a circular spawning-inducing pond, and placing the mature Gymnothorax przewalskii at a density of 4kg/m3The water flow is 6L/min, the water temperature in the spawning pool is controlled at 14 ℃, the dissolved oxygen of the water body is 7.5mg/L, and the pH value of the water is 8.0; firstly, injecting a first oxytocin compatibility (the components are 4 mug/kg of luteinizing hormone releasing hormone analogue and 6mg/kg of diutanone maleate) only for female fish in an oxytocin parent; continuing to inject a second oxytocin formulation (comprising 12 μ g/kg lutein release hormone analogue, 10mg/kg diutanone maleate and 1000 IU/kg chorionic gonadotropin) into the female fish 24h after the injection; injecting a third oxytocic composition (comprising 5 μ g/kg of lutein-releasing hormone analogue, 5mg/kg of diutanone maleate and 500 international units/kg of chorionic gonadotropin) into male fish in the oxytocic parent; checking the ovulation condition of the parent fish after 56 hours, slightly pressing the belly of the female fish to discharge fish eggs, lifting the female fish, wrapping the female fish with a towel, wiping off body surface moisture, squeezing the fish eggs into a basin, lifting the male fish, wrapping the male fish with a towel, wiping off the body surface moisture, squeezing semen into the basin, slightly stirring the semen with feathers for 1 minute, adding clear water, standing for 2 minutes, washing the eggs with clear water for 5 times, and washing off redundant semen to obtain fertilized eggs; placing fertilized eggs in an incubation frame in a circulating water culture system, and incubating by using running water, wherein the length and the width of the incubation frame are 45 and 35cm, 4000 fertilized eggs are placed in each frame, and the eggs are not overlapped; the flow rate of water is 6L/min, the dissolved oxygen of water is 8.5mg/L, the pH value of water is 8.0, the incubation water temperature is 9.5 ℃, fertilized eggs are kept in a static state in an incubation frame, dead eggs are cleaned in time in the incubation process, the information of good water temperature, water quality and the like is monitored and recorded, and the larvae of the Gymnothorax parva are obtained after incubation for 17 days.
In example 3, the spawning induction rate of the aphelenchoides farinae is 80%, the fertilization rate is 94.67%, and the hatchability rate is 91.83%.
The features of the embodiments and embodiments described herein above may be combined with each other without conflict.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (6)
1. An artificial propagation method of Gymnocypris ventricosa, which is characterized by comprising the following steps:
s101, parent fish breeding: selecting the wild aphyllobelia affinis with no damage to the body surface and the weight of 400-1300g as parent fishes for breeding, wherein the daily feeding amount of the feed in the breeding process is 0.1-1% of the weight of the parent fishes;
s102, parent fish selection: selecting mature Gymnothorax biddulphi with no disease and strong constitution as an induced spawning parent;
s103, artificial induced spawning: firstly, injecting a first oxytocic compatibility only for female fish in an oxytocic parent; after 24 hours, continuing to inject a second oxytocic for compatibility with the female fish, and injecting a third oxytocic for compatibility with the male fish in the oxytocic parent;
s104, artificial insemination: temporarily breeding induced spawning parents in an induced spawning pond at the water temperature of 9-14 ℃, checking female fish in the induced spawning parents every 8 hours after 48 hours, slightly pressing the belly of the female fish, squeezing fish eggs into a basin if fish eggs are produced, squeezing sperm of male fish into the basin, stirring, standing and cleaning to obtain fertilized eggs;
s105, hatching: and (5) hatching the fertilized eggs obtained in the step S104 by using running water to obtain the larval fish of the Gymnocypris ventricosa.
2. The artificial propagation method of Gymnocypris ventricosa according to claim 1, the first oxytocin compatibility comprises a luteinizing hormone releasing hormone analogue with the dosage of 3-8 mug/kg and diutanone maleate with the dosage of 2-6mg/kg, the second oxytocin formulation comprises a lutein releasing hormone analogue with a dosage of 10-12 mug/kg, diutanone maleate with a dosage of 6-10mg/kg and chorionic gonadotropin with a dosage of 500-1000 international units/kg, the third oxytocin formulation comprises a lutein-releasing hormone analogue in a dosage of 5-6 mug/kg, diutanone maleate in a dosage of 3-5mg/kg and chorionic gonadotropin in a dosage of 250-500 international units/kg.
3. The artificial propagation method of Gymnocypris ventricosa as claimed in claim 1, wherein the parent fish is cultured at a density of 3.5kg/m in step S1013The pH value of the water for culturing the parent fish is 7.6-8.4, the water flow is 15-20L/min, the water dissolved oxygen is 6-10mg/L, and the water temperature is 4-17 ℃.
4. The artificial propagation method of Gymnocypris ventricosa as claimed in claim 1, wherein in step S101, the protein content of the feed used in the cultivation process is 36-41%, and the feed is fed 2 times a day at 9 am and 6 pm respectively.
5. The artificial propagation method of Gymnocypris ventricosa, according to claim 1, characterized in that, in step S102, the selected mature Gymnocypris ventricosa is placed in an induced spawning pond, the ratio of male and female parent fishes is 1:1, 2-10 pairs of parent fishes are selected each time, the water temperature in the induced spawning pond is 9-14 ℃, the pH value of the water is 7.8-8.2, the water flow is 20-30L/min, and the water dissolved oxygen is 6-10 mg/L.
6. The artificial propagation method of aphelenchoides farinae as claimed in claim 1, wherein in step S105, the length and width of the hatching frame used in the hatching process is 45 cm to 35cm, and at most 3000 + 5000 eggs are put into each hatching frame without overlapping; the water temperature during incubation is 9-12 deg.C, incubation time is 14-17 days, water flow is 4.5-6L/min, water dissolved oxygen is 6-10mg/L, and pH value of water is 7.8-8.2.
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