CN111066785B - Application of DMNT in preparation of lepidoptera pest insecticide - Google Patents
Application of DMNT in preparation of lepidoptera pest insecticide Download PDFInfo
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- CN111066785B CN111066785B CN201911361655.0A CN201911361655A CN111066785B CN 111066785 B CN111066785 B CN 111066785B CN 201911361655 A CN201911361655 A CN 201911361655A CN 111066785 B CN111066785 B CN 111066785B
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- LUKZREJJLWEWQM-UHFFFAOYSA-N DMNT Natural products CC(C)=CCCC(C)=CC=C LUKZREJJLWEWQM-UHFFFAOYSA-N 0.000 title claims abstract description 55
- QXSIYYQGSPCIHN-UHFFFAOYSA-N 1,1-dimethyl-2-[4-(5-nitrofuran-2-yl)-1,3-thiazol-2-yl]hydrazine Chemical compound S1C(NN(C)C)=NC(C=2OC(=CC=2)[N+]([O-])=O)=C1 QXSIYYQGSPCIHN-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 39
- 241000255777 Lepidoptera Species 0.000 title claims abstract description 14
- 239000002917 insecticide Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims description 9
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 19
- 241000346285 Ostrinia furnacalis Species 0.000 claims abstract description 15
- 241000256250 Spodoptera littoralis Species 0.000 claims abstract description 4
- 239000000839 emulsion Substances 0.000 claims description 4
- 239000002552 dosage form Substances 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000000575 pesticide Substances 0.000 abstract description 16
- 241000196324 Embryophyta Species 0.000 abstract description 9
- 230000000607 poisoning effect Effects 0.000 abstract description 7
- 230000006378 damage Effects 0.000 abstract description 5
- 230000018109 developmental process Effects 0.000 abstract description 5
- 230000032669 eclosion Effects 0.000 abstract description 5
- 230000019617 pupation Effects 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 230000001850 reproductive effect Effects 0.000 abstract description 3
- 239000002689 soil Substances 0.000 abstract description 3
- 239000006272 natural pesticide Substances 0.000 abstract description 2
- 229930000044 secondary metabolite Natural products 0.000 abstract description 2
- 241000256251 Spodoptera frugiperda Species 0.000 description 9
- 240000008042 Zea mays Species 0.000 description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- 235000005822 corn Nutrition 0.000 description 6
- 238000005457 optimization Methods 0.000 description 6
- 239000012188 paraffin wax Substances 0.000 description 4
- 235000003642 hunger Nutrition 0.000 description 3
- 239000002574 poison Substances 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241000219193 Brassicaceae Species 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 239000005662 Paraffin oil Substances 0.000 description 2
- 238000007385 chemical modification Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000035929 gnawing Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 231100000572 poisoning Toxicity 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- XARSCEOUIZQPSI-UHFFFAOYSA-N 8-ethyloxocan-2-one Chemical compound CCC1CCCCCC(=O)O1 XARSCEOUIZQPSI-UHFFFAOYSA-N 0.000 description 1
- 206010004716 Binge eating Diseases 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 208000032841 Bulimia Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241001147398 Ostrinia nubilalis Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000500441 Plutellidae Species 0.000 description 1
- 208000028990 Skin injury Diseases 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 101000693622 Spodoptera frugiperda Allatotropin Proteins 0.000 description 1
- -1 Terpene compound Chemical class 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000014679 binge eating disease Diseases 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an application of DMNT in preparing lepidoptera pest insecticide, belonging to the technical field of pesticide manufacture. DMNT is a natural secondary metabolite of plants and has no damage effect on the plants. The prior art shows that DMNT has volatility and can attract natural enemies of pests to prey on the pests. The invention discovers for the first time that the DMNT has a poisoning effect on three lepidoptera pests including diamond back moth, corn borer and spodoptera littoralis, can obviously reduce the pupation rate and eclosion rate of the pests and reduce the reproductive capacity of the pests. And the substance is easy to volatilize and metabolize, and cannot pollute soil and water sources, so that the development of a novel high-efficiency pollution-free natural pesticide by taking the substance as a research object has important significance for preventing and controlling crop pests.
Description
Technical Field
The invention belongs to the technical field of pesticide manufacture, and particularly relates to an application of DMNT in preparation of lepidoptera pest pesticide.
Background
Common crops of cruciferae crops include various vegetables such as oil crops rape and cabbage, and the main pest of the cruciferae crops is Plutella Xylostella (PX). The diamondback moth, also known as Trichostoma molitor, is one of main lepidoptera pests, and the early larvae gnaw the leaves to form holes and notches, and after the leaves grow to 3 years, the feeding capacity is greatly enhanced, and the 4-year larvae enter a binge eating period, parasitic plant leaves can be eaten into a net shape. The diamondback moth has short life cycle, high propagation speed and strong environmental adaptability, can cause insect pest outbreak in a short time and seriously harms the growth of economic crops.
The corn borer (Ostrinia furnacalis), also known as borer, belongs to lepidoptera and is a major pest of corn, and it parasitizes at the young part of the plant in the larval stage before the fourth instar stage, mostly adheres to the heart leaves, and forms holes after gnawing. The gnawing ability of the larvae in the later stage of four ages is greatly enhanced, the larvae are transferred to the outside of a plant from heart and leaves, and mainly drill corn stems and leaves, so that the stems and the leaves can be broken when the larvae are serious, the corn yield is reduced, the quality of corn grains is reduced, and great economic loss is caused to farmers.
Spodoptera frugiperda (Spodoptera frugiperda) belongs to Lepidoptera, is mainly distributed in overseas areas such as south America and the like in early years, invades China from southeast Asia in 2019 in 1 month, is discovered in provinces and cities at present, and does not have an effective pesticide. Spodoptera frugiperda larvae are omnivorous and mainly harm commercial crops such as corn, rice, sorghum and the like. The larva has a large size, can gnaw the leaves of crops into a sieve shape by the early larva, and can gnaw the stems and the stalks of the crop seedlings to be broken by the late larva, so that various economic crops are damaged.
Therefore, the control of the three pests is very important. However, most of the existing pesticides on the market are organic phosphorus chemical agents, and have the following disadvantages: (1) the odor is strong and unpleasant, the inhalation of the odor can cause respiratory tract injury, and the mistaking of the medicament on the skin can cause skin injury. (2) Pesticide residues seriously pollute water resources and soil environment and are difficult to completely degrade within a few years. (3) People and animals mistakenly eat fruits or leaves sprayed with the pesticide, which can cause serious harm. (4) Pests are prone to develop resistance to drugs. With the gradual manifestation of the defects of chemical pesticides, natural plant pesticides receive more and more attention, and the exploration and development of natural and efficient pesticides become the key points for solving the insect pest problem.
It is well known that BT toxic proteins are effective in poisoning pests. With the wide application in production, various pests have developed drug resistance to BT protein, and the development of novel biological pesticides is the demand guide of the current market.
Terpene compound 4,8-dimethyl-1,3, 7-nonane (4,8-dimethyl-1,3, 7-nonanolide), molecular formula C11H18The compound can be used as an inducer to induce natural enemies of pests to catch and kill the pests or induce plant bodies to generate proteins which cannot be digested by the pests, but the DMNT has no literature report that the DMNT has the effect of directly poisoning and killing the pests.
Disclosure of Invention
The invention aims to solve the problems and provides application of DMNT in preparing lepidoptera pest insecticide.
The invention realizes the purpose through the following technical scheme:
an application of DMNT in preparing lepidoptera pest insecticide.
As a further optimization scheme of the invention, the application of the DMNT as the only active ingredient in the preparation of pesticides, wherein the DMNT is 4,8-dimethyl-1,3, 7-nonane triene or a compound generated after conventional chemical modification based on 4,8-dimethyl-1,3, 7-nonane triene.
As a further optimization scheme of the invention, the pesticide is used for killing lepidoptera pests such as diamondback moth, corn borer and spodoptera littoralis.
As a further optimization scheme of the invention, the insecticide with the DMNT dosage of more than 7.5nM is adopted when the larvae of the diamondback moth are poisoned.
As a further optimization scheme of the invention, the pesticide with the dosage of more than 100nM DMNT is adopted when the larvae of the corn borer before 4 th instar are poisoned.
As a further optimization scheme of the invention, 1.3 mu M of DMNT pesticide is adopted when killing Spodoptera frugiperda larvae before 3 years old.
As a further optimization scheme of the invention, the pesticide is prepared into a conventional dosage form for use, and the conventional dosage form comprises emulsion, powder and the like, but is not limited to the emulsion, the powder and the like. Since DMNT is volatile and insoluble in water, it is further preferable that DMNT is used as an insecticide after being prepared into an emulsion.
The invention has the beneficial effects that: DMNT is a natural secondary metabolite of plants and has no damage effect on the plants. The prior art shows that DMNT has volatility and can attract natural enemies of pests to prey on the pests. The invention discovers for the first time that the DMNT has a poisoning effect on three lepidoptera pests including diamond back moth, corn borer and spodoptera littoralis, can obviously reduce the pupation rate and eclosion rate of the pests and reduce the reproductive capacity of the pests. And the substance is easy to volatilize and metabolize, and cannot pollute soil and water sources, so that the development of a novel high-efficiency pollution-free natural pesticide by taking the substance as a research object has important significance for preventing and controlling crop pests.
Drawings
FIG. 1 is a graph of the mortality results of diamondback moth larvae after DMNT feeding;
FIG. 2 is a graph showing the results of pupation of Plutella xylostella after feeding DMNT;
FIG. 3 is a graph of the results of the eclosion rate of plutella xylostella after feeding DMNT;
FIG. 4 is a graph of mortality results for corn borer after DMNT feeding;
FIG. 5 is a graph of the results of the rate of debarking by 3 rd instar corn borer larvae following DMNT feeding;
FIG. 6 is a graph of mortality results from Spodoptera frugiperda at 3 years after feeding DMNT.
Detailed Description
The present application will now be described in further detail with reference to the drawings, it should be noted that the following detailed description is given for illustrative purposes only and is not to be construed as limiting the scope of the present application, as those skilled in the art will be able to make numerous insubstantial modifications and adaptations to the present application based on the above disclosure.
Example 1: the DMNT has poisoning effect on diamondback moth which is a cruciferous crop pest.
(1) DMNT was dissolved in paraffin and diluted to a sample concentration of 75 ng/. mu.l for use.
(2) Starving 4-day-old diamondback moths for 1 h.
(3) The experiment was set up with 6 parallel replicates for each group, as well as the control group and the experimental group. Using a punch to drill uniform sized feeds, three feeds were added to each plate, and 5. mu.l of paraffin oil (control) or 5. mu.l of DMNT sample (experimental) was added to each feed, i.e., each feed contained 375ng of DMNT, 15 larvae of Plutella xylostella were added to each group and cultured in a 25 ℃ incubator.
(4) And (5) counting the death rate of the diamondback moth after 12h and 20h after treatment, and the pupation rate and the eclosion rate of the diamondback moth at the later stage.
The experimental results show that with increasing time of DMNT feeding, mortality of diamondback moth larvae increased significantly (fig. 1), pupation rate (fig. 2) and eclosion rate decreased significantly (fig. 3) compared to the control.
Experimental results show that the DMNT can effectively poison the diamondback moth and reduce the reproductive capacity of the diamondback moth.
Example 2: the toxic effect of DMNT to corn borer.
(1) DMNT was dissolved in paraffin and diluted to a concentration of 1. mu.g/. mu.l for use.
(2) Starvation treatment is carried out on 3-year-old 1-day corn borers for 1 h.
(3) The experiment was set up in 6 replicates for the control and experimental groups, respectively. Using a punch, three pieces of uniform-sized feeds were placed on each plate, 15. mu.l of paraffin oil (control group) or 15. mu.l of DMNT sample (experimental group) was added to each feed, that is, each feed of the experimental group contained 15. mu.g of DMNT, and 15 larvae of corn borer, 3 instars and 1 day old, were placed in each group. And (4) culturing the treated larvae of the ostrinia nubilalis in a constant-temperature incubator at 25 ℃.
(4) The mortality rate of the corn borers and the molting rate of 3-4-instar larvae are counted at 48h and 72h after feeding respectively.
The experimental results show that the mortality rate of 3 rd corn borer larvae is remarkably increased and the molting rate of 3 rd to 4 th transition period larvae is remarkably reduced (figure 5) compared with the control along with the increase of DMNT feeding time.
Experimental results show that the DMNT can effectively poison corn borer larvae, delay molting stages and delay development of the larvae.
Example 3: poisoning effect of DMNT on Spodoptera frugiperda
(1) Fresh corn leaves are taken, and 100 mu l of paraffin (control group) and diluted 1 mu g/mu l of DMNT (experimental group) are uniformly smeared on the leaves respectively, namely each leaf of the experimental group contains 100 mu g of DMNT.
(2) Starving 3-instar spodoptera frugiperda larvae for 1 h.
(3) 3 Spodoptera frugiperda larvae were added to the control group (paraffin) and the experimental group (DMNT), respectively, and 3 biological replicates were set.
(4) And (3) putting the treated spodoptera frugiperda into a constant-temperature incubator at 25 ℃.
The experimental results showed that no mortality occurred in the larvae of the control group and the mortality rate of the larvae of the experimental group exceeded 60% (fig. 6). The DMNT can effectively poison Spodoptera frugiperda larvae.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several modifications and improvements can be made, for example, DMNT may have poisoning effect on other pests, and effective pesticide preparations produced by various chemical modifications based on DMNT are also included in the scope of the present invention.
Claims (4)
1. An application of DMNT in preparing insecticide for lepidoptera pests, which is used for killing diamondback moth, corn borer and spodoptera littoralis which are lepidoptera pests, is characterized in that the DMNT is 4,8-dimethyl-1,3, 7-nonane triene.
2. The use of a DMNT according to claim 1 in the preparation of a lepidopteran pest insecticide, wherein said DMNT is used as the sole active ingredient in the preparation of an insecticide.
3. The use of DMNT in the preparation of a lepidopteran pest insecticide as claimed in claim 1, wherein said insecticide is formulated for use in a conventional dosage form.
4. The use of DMNT in the preparation of a lepidopteran pest insecticide according to claim 3, wherein said formulation is an emulsion.
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| CN111202063B (en) * | 2020-02-25 | 2021-04-13 | 北京市农林科学院 | Bemisia tabaci oviposition repellent |
| CN112136811B (en) * | 2020-10-09 | 2021-09-14 | 安徽农业大学 | Application of TMTT (Tetramethyltetrathionylmethane-tetrathionylmethane) as medicine for resisting lepidoptera pests from attacking crops |
| CN115363028B (en) * | 2022-08-18 | 2023-04-11 | 安徽农业大学 | Application of DMNT structure modified product in preparing medicine for resisting lepidoptera pests to damage crops |
| CN115491425A (en) * | 2022-10-26 | 2022-12-20 | 安徽农业大学 | Spodoptera frugiperda acetylcholinesterase A201S and F290V mutation detection method and application |
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| CN103563953A (en) * | 2012-08-05 | 2014-02-12 | 南京华洲药业有限公司 | Composite insecticidal composition containing methoxyfenozide and chlorfluazuron and application thereof |
| CN103621532A (en) * | 2012-08-27 | 2014-03-12 | 南京华洲药业有限公司 | Synergistic insecticidal composition containing thiamethoxam and tolfenpyrad and application thereof |
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