CN103859210A - Artificial medium of trichogramma - Google Patents
Artificial medium of trichogramma Download PDFInfo
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- CN103859210A CN103859210A CN201410058820.6A CN201410058820A CN103859210A CN 103859210 A CN103859210 A CN 103859210A CN 201410058820 A CN201410058820 A CN 201410058820A CN 103859210 A CN103859210 A CN 103859210A
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- trichogramma
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention discloses an artificial medium of trichogramma. The artificial medium comprises the following ingredients in mass fraction: 19-40% of insect pupae hemolymph, 17-38% of egg yolk, 10-33% of milk, 4-19% of a Nissl salt solution, 0.8-8% of trehalose, 2-15% of a ganoderan solution with a mass fraction of 5% and 2-15% of a chitosan oligosaccharide solution with a mass fraction of 3.6%. By adopting the artificial medium of trichogramma, the breeding effect of trichogramma is obviously better than that of a traditional artificial medium through the trehalose, the ganoderan solution and the chitosan oligosaccharide solution via effective composition, and the content of insects can be reduced, so that the breeding cost of trichogramma is reduced. Through the artificial medium of trichogramma, trichogramma can be bred by subculturing, no degradation phenomenon exists, and the breeding effect is stable. By adopting the artificial medium of trichogramma, various kinds of trichogramma such as trichogramma dendrolimi, trichogramma confusum, Asian corn borer trichogramma, European corn borer trichogramma and geographical populations thereof can be bred, and good effects are realized.
Description
Technical field:
The invention belongs to insect and raise field, be specifically related to a kind of trichogramma synthetic medium.
Background technology:
Trichogramma Trichogramma belongs to Hymenoptera Trichogrammatidae, its aboundresources, widely distributed, it is a kind of important parasitic enemy insect resource, since the '20s in last century, be widely used in the biological control of multiple agriculture and forestry injurious insect, remarkable to insect control effect, produce in grain security, in pollution-free food, organic foodstuff production, bring into play important function.At present, use the ovum breeding oophagous trichogrammae of the insects such as rice moth, gelechiid, powder snout moth's larva, tussah, castor silkworm and pine moth both at home and abroad, but owing to being subject to various condition restriction, supply falls short of demand and reproductive-cost sharply increases for host insect ovum, become the outstanding problem that trichogramma utilizes.Therefore, utilize synthetic medium captive breeding trichogramma to seem particularly important.
Chinese scholar was successfully utilized artificial eggs to breed out trichogramma dendrolimi (T.dendrolimi) and intends the trichogrammas such as Australia trichogramma T.confusum (the yellow trichogramma T.chilois of=snout moth's larva), Ostrinia furnacalis trichogramma (T.ostriniae) in the eighties in last century, showed that through field test the trichogramma that first two artificial eggs is bred can successfully prevent and treat the important agriculture and forestry injurious insects such as bollworm, sugarcane moth borer, pine moth.But owing to failing to develop the synthetic medium of a large amount of external breeding oophagous trichogrammaes of energy, the extracorporeal culture technology that connects generation does not make a breakthrough temporarily.For insect material in synthetic medium (pupa hemolymph) content higher (more than 40%), the problem such as numerous honeybee quality is unstable, adult eclosion rate is lower, viability is weak, Continuous Cultivation is more difficult, urgently need to carry out the synthetic medium of development high-quality.
Summary of the invention:
The object of this invention is to provide a kind of content that can either reduce insect material in synthetic medium, can guarantee again trichogramma quality, keep stable and can connect generation, cultivation in large quantities, adapt to the trichogramma synthetic medium of the needs to natural enemy insect in agricultural production biological control of insect pests.
Trichogramma synthetic medium of the present invention, it is characterized in that, by gross mass mark 100%, comprise insect pupa hemolymph 19%-40%, egg yolk 17%-38%, milk 10%-33%, Nissl salting liquid 4%-19%, trehalose 0.8%-8%, mass fraction 5% GL-B solution 2%-15% and mass fraction 3.6% chitosan oligosaccharide solution 2%-15%.
Preferably, can also in above-mentioned trichogramma synthetic medium, add antibiotic as the material such as penicillin and streptomysin, addition be about 200-400 units per ml.
Further preferably, described trichogramma synthetic medium, by gross mass mark 100%, comprise insect pupa hemolymph 26%-33%, egg yolk 23%-28%, milk 18%-20%, Nissl salting liquid 8%-14%, trehalose 1.8%-5%, mass fraction 5% GL-B solution 5%-12% and mass fraction 3.6% chitosan oligosaccharide solution 2%-5%.
The one of the described optional tussah chrysalis hemolymph of insect pupa hemolymph, castor-oil plant silkworm chrysalis hemolymph, pine caterpillar hemolymph or lopper worm pupa hemolymph, preferably tussah chrysalis hemolymph.
It is the one in 10% defatted milk, fresh milk or full-cream plain chocolate that described milk can be selected the mass percent that skimmed milk power is made into, and is preferably mass fraction 10% defatted milk.
Described Nissl salting liquid is material of the prior art, and its every liter formula is: 7.5g NaCl, 0.1g KCl, 0.2g CaCl, 0.2g NaHCO
3, surplus is water.
Trichogramma synthetic medium of the present invention, by effective prescription, trehalose in formula and GL-B-chitosan oligosaccharide solution are better than traditional synthetic medium to the cultivation successful of trichogramma, and can reduce the content of insect material, thereby reduce the cost of breeding oophagous trichogrammae.Trichogramma synthetic medium of the present invention, in order to the breeding oophagous trichogrammae cultivation of going down to posterity, is not found degradation phenomena, cultivates effect stability.Trichogramma synthetic medium of the present invention can be raised multiple trichogramma, and as trichogramma kind and geographical population thereof such as trichogramma dendrolimi, plan Australia trichogramma, Ostrinia furnacalis trichogramma, European corn borer trichogrammas, effect is all very good.
The specific embodiment:
Following examples are to further illustrate of the present invention, rather than limitation of the present invention.
GL-B (Fu Shi GL-B, purchased from rich bio tech ltd, Wuhan) in following examples and chitosan oligosaccharide (purchased from Zhejiang Province gold shell Biochemie Co., Ltd) are mixed with the aqueous solution of mass percent 5% and mass percent 3.6% with sterilized water.
Embodiment 1:
The trichogramma synthetic medium of the present embodiment, by gross mass mark 100%, comprise tussah chrysalis hemolymph 30%, egg yolk 25%, mass fraction 10% defatted milk 20%, Nissl salting liquid 10%, trehalose 5%, mass fraction 5% GL-B solution 5% and mass fraction 3.6% chitosan oligosaccharide solution 5%.According to the ratio in above-mentioned formula, above-mentioned substance is mixed, be the trichogramma synthetic medium of the present embodiment.
With the trichogramma synthetic medium extracorporeal culture trichogramma dendrolimi of the present embodiment, average every honeybee card obtains 95% parasitic rate, 100% percentage of pupation, 84.4% eclosion rate, 89.6% normal honeybee rate and 911 normal bee eclosion amounts.Raised continuously for 25 generations and do not find above population degradation phenomena.
By mass fraction 100%, with the trichogramma of the synthetic medium extracorporeal culture that contains 30% tussah chrysalis hemolymph, 30% egg yolk, 20% fresh milk, 10% Nissl salting liquid and 10% trehalose, average every honeybee card obtains 98.9% parasitic rate, 77.7% percentage of pupation, 77.2% eclosion rate, 80% normal honeybee rate and 266 normal bee eclosion amounts.
As can be seen here, add after GL-B solution and chitosan oligosaccharide solution, the cultivation successful of trichogramma is better than to traditional synthetic medium, and can reduces the content of insect material, thus the cost of reduction breeding oophagous trichogrammae.
Intend Australia trichogramma, Trichogramma Ostriniae, European corn borer trichogramma or Trichogramma brassicae with the trichogramma synthetic medium extracorporeal culture of the present embodiment and also obtained identical effect.
Embodiment 2:
The trichogramma synthetic medium of the present embodiment, by gross mass mark 100%, comprise tussah chrysalis hemolymph 26.8%, egg yolk 23.8%, mass fraction 10% defatted milk 19.2%, Nissl salting liquid 13.4%, trehalose 1.8%, mass fraction 5% GL-B solution 12% and mass fraction 3.6% chitosan oligosaccharide solution 3%.According to the ratio in above-mentioned formula, above-mentioned substance is mixed, be the trichogramma synthetic medium of the present embodiment.
With the trichogramma synthetic medium extracorporeal culture trichogramma dendrolimi of embodiment, average every honeybee card obtains 92.0% parasitic rate, 99.2% percentage of pupation, 76.8% eclosion rate, 82.5% normal honeybee rate and 450 normal bee eclosion amounts.Raised continuously for 20 generations and do not find above population degradation phenomena.
By mass fraction 100%, with the trichogramma of the synthetic medium extracorporeal culture that contains 25% tussah chrysalis hemolymph, 30% egg yolk, 20% fresh milk, 10% Nissl salting liquid and mass fraction 5% GL-B solution 12% and 3.6% GL-B solution 3%, average every honeybee card obtains 78.3% parasitic rate, 89% percentage of pupation, 69% eclosion rate, 67% normal honeybee rate and 322 normal bee eclosion amounts.
Intend Australia trichogramma, Trichogramma Ostriniae, European corn borer trichogramma or Trichogramma brassicae with the trichogramma synthetic medium extracorporeal culture of the present embodiment and also obtained identical effect.
Embodiment 3:
The trichogramma synthetic medium of the present embodiment, by gross mass mark 100%, comprise tussah chrysalis hemolymph 32.5%, egg yolk 28%, mass fraction 10% defatted milk 18%, Nissl salting liquid 8.2%, trehalose 3.3%, mass fraction 5% GL-B solution 8% and mass fraction 3.6% chitosan oligosaccharide solution 2%.According to the ratio in above-mentioned formula, above-mentioned substance is mixed, be the trichogramma synthetic medium of the present embodiment.
With the trichogramma synthetic medium extracorporeal culture trichogramma dendrolimi of the present embodiment, average every honeybee card obtains 97% parasitic rate, 98.8% percentage of pupation, 79.4% eclosion rate, 87.5% normal honeybee rate and 810 normal bee eclosion amounts.Raised continuously for 20 generations and do not find above population degradation phenomena.
By mass fraction 100%, with the trichogramma of the synthetic medium extracorporeal culture that contains tussah chrysalis hemolymph 30%, egg yolk 30%, fresh milk 20%, Nissl salt 10% and mass fraction 5% GL-B solution 5% and 3.6 chitosan oligosaccharide solution 5%, average every honeybee card obtains 100% parasitic rate, 92.7% percentage of pupation, 79.3% eclosion rate, 81.7% normal honeybee rate and 653 normal bee eclosion amounts.
Intend Australia trichogramma, Trichogramma Ostriniae, European corn borer trichogramma or Trichogramma brassicae with the trichogramma synthetic medium extracorporeal culture of the present embodiment and also obtained identical effect.
Embodiment 4:
The trichogramma synthetic medium of the present embodiment, by gross mass mark 100%, comprise tussah chrysalis hemolymph 19%, egg yolk 38%, mass fraction 10% defatted milk 10%, Nissl salting liquid 19%, trehalose 0.8%, mass fraction 5% GL-B solution 2% and mass fraction 3.6% chitosan oligosaccharide solution 11.2%.According to the ratio in above-mentioned formula, above-mentioned substance is mixed, be the trichogramma synthetic medium of the present embodiment.
With the trichogramma synthetic medium In Vitro Rearing of Trichogramma of the present embodiment, its effect is very good, and the cultivation of going down to posterity, does not find degradation phenomena, cultivates effect stability.
Embodiment 5:
The trichogramma synthetic medium of the present embodiment, by gross mass mark 100%, comprise tussah chrysalis hemolymph 21%, egg yolk 17%, mass fraction 10% defatted milk 33%, Nissl salting liquid 4%, trehalose 8%, mass fraction 5% GL-B solution 15% and mass fraction 3.6% chitosan oligosaccharide solution 2%.According to the ratio in above-mentioned formula, above-mentioned substance is mixed, be the trichogramma synthetic medium of the present embodiment.
With the trichogramma synthetic medium In Vitro Rearing of Trichogramma of the present embodiment, its effect is very good, and the cultivation of going down to posterity, does not find degradation phenomena, cultivates effect stability.
Embodiment 6:
The trichogramma synthetic medium of the present embodiment, by gross mass mark 100%, comprise tussah chrysalis hemolymph 40%, egg yolk 17%, mass fraction 10% defatted milk 21.2%, Nissl salting liquid 4%, trehalose 0.8%, mass fraction 5% GL-B solution 2% and mass fraction 3.6% chitosan oligosaccharide solution 15%.According to the ratio in above-mentioned formula, above-mentioned substance is mixed, be the trichogramma synthetic medium of the present embodiment.
With the trichogramma synthetic medium In Vitro Rearing of Trichogramma of the present embodiment, its effect is very good, and the cultivation of going down to posterity, does not find degradation phenomena, cultivates effect stability.
Claims (5)
1. a trichogramma synthetic medium, it is characterized in that, by gross mass mark 100%, comprise insect pupa hemolymph 19%-40%, egg yolk 17%-38%, milk 10%-33%, Nissl salting liquid 4%-19%, trehalose 0.8%-8%, mass fraction 5% GL-B solution 2%-15% and mass fraction 3.6% chitosan oligosaccharide solution 2%-15%.
2. trichogramma synthetic medium according to claim 1, is characterized in that, can also in trichogramma synthetic medium, add penicillin and/or streptomysin, addition is 200-400 units per ml.
3. trichogramma synthetic medium according to claim 1, it is characterized in that, described trichogramma synthetic medium, by gross mass mark 100%, comprise insect pupa hemolymph 26%-33%, egg yolk 23%-28%, milk 18%-20%, Nissl salting liquid 8%-14%, trehalose 1.8%-5%, mass fraction 5% GL-B solution 5%-12% and mass fraction 3.6% chitosan oligosaccharide solution 2%-5%.
4. trichogramma synthetic medium according to claim 1, is characterized in that, described insect pupa hemolymph is the one of tussah chrysalis hemolymph, castor-oil plant silkworm chrysalis hemolymph, pine caterpillar hemolymph or lopper worm pupa hemolymph.
5. trichogramma synthetic medium according to claim 1, is characterized in that, described milk is that the mass percent that skimmed milk power is made into is the one in 10% defatted milk, fresh milk or full-cream plain chocolate.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104187206A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Nutritional liquid for trichogramma and preparation method and application of nutritional liquid |
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN108402343A (en) * | 2018-04-09 | 2018-08-17 | 华南农业大学 | A kind of man-made feeds formula of Predacious function |
| CN109526884A (en) * | 2018-12-14 | 2019-03-29 | 云南省烟草公司玉溪市公司 | A method of culture Steinernema carpocapsae nematode |
| CN109644945A (en) * | 2018-12-14 | 2019-04-19 | 广东省生物资源应用研究所 | A kind of method of fast quick-recovery Steinernema carpocapsae infection phase nematode |
| CN112119980A (en) * | 2020-08-31 | 2020-12-25 | 广东省科学院动物研究所 | Low-temperature propagation method for in vitro culture of trichogramma |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105145487A (en) * | 2014-07-23 | 2015-12-16 | 中国科学院动物研究所 | Trichogramma japonicun ashmead artifical breeding production method |
| CN104187206A (en) * | 2014-09-26 | 2014-12-10 | 张可池 | Nutritional liquid for trichogramma and preparation method and application of nutritional liquid |
| CN108402343A (en) * | 2018-04-09 | 2018-08-17 | 华南农业大学 | A kind of man-made feeds formula of Predacious function |
| CN108402343B (en) * | 2018-04-09 | 2022-03-22 | 华南农业大学 | Artificial feed formula for harris baccarum |
| CN109526884A (en) * | 2018-12-14 | 2019-03-29 | 云南省烟草公司玉溪市公司 | A method of culture Steinernema carpocapsae nematode |
| CN109644945A (en) * | 2018-12-14 | 2019-04-19 | 广东省生物资源应用研究所 | A kind of method of fast quick-recovery Steinernema carpocapsae infection phase nematode |
| CN109644945B (en) * | 2018-12-14 | 2022-07-26 | 广东省生物资源应用研究所 | Method for rapidly recovering nematodes in Steinernema carpocapsae infection stage |
| CN109526884B (en) * | 2018-12-14 | 2022-07-26 | 云南省烟草公司玉溪市公司 | Method for culturing Steinernema carpocapsae nematodes |
| CN112119980A (en) * | 2020-08-31 | 2020-12-25 | 广东省科学院动物研究所 | Low-temperature propagation method for in vitro culture of trichogramma |
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