CN108496970A - A kind of composition, preparation and its application for desinsection - Google Patents
A kind of composition, preparation and its application for desinsection Download PDFInfo
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- CN108496970A CN108496970A CN201810447693.7A CN201810447693A CN108496970A CN 108496970 A CN108496970 A CN 108496970A CN 201810447693 A CN201810447693 A CN 201810447693A CN 108496970 A CN108496970 A CN 108496970A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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Abstract
The invention discloses a kind of composition for desinsection, the composition is (1 20) by weight ratio：The component A and component B compositions of (1 10), component A is jinggangmeisu, and component B is Tea Saponin.The invention also discloses another compositions, and on the basis of aforementioned composition, component A is made of jinggangmeisu and Pseuomonas denitrifican, and the weight ratio of jinggangmeisu and Pseuomonas denitrifican is 3:1.Composition provided by the invention can improve the targeting to pest, reduce the damage to natural enemy, slow down the generation of resistance, have the function that synergy, while may additionally facilitate plant growth, improve crop quality.
Description
Technical field
The present invention relates to pesticide field, specially a kind of compositions, preparation and its application for desinsection.
Background technology
Organic chemical pesticides prevent pest and disease damage for the mankind and provide strong weapon, substantially increase the production of crops
Amount and quality, but the series of problems such as long-term disruption of ecological balance, Environmental security for relying on and being brought using chemical pesticide, hinder agriculture
The sustainable development helped already.
The use of pesticide also leads to drastically reduction or even more the day hostile pesticide of natural enemy quantity while killing pest
The far super pest of susceptibility itself, the low of targeting cause insecticidal effect unsatisfactory.Insecticide, resistance is used alone simultaneously
Problem is serious, has studies have shown that high temperature damp-heat area in south China, the resistance of insecticidal bactericide seriously to enhance.And pesticide
It is big using endangering water body and soil environment, the growth of crop is also inhibited while desinsection, and human and environment is also resulted in
Damage.
Invention content
The present invention provides a kind of composition for desinsection, and composition is made of component jinggangmeisu and Tea Saponin, described
Composition can improve the targeting to pest, reduce the damage to natural enemy, slow down the generation of resistance, reach synergistic work
With, while plant growth is may additionally facilitate, improve crop quality.
To solve the above problems, the present invention provides a kind of composition for desinsection, the composition is by weight ratio
(1-20)：The component A and component B compositions of (1-10), the component A is jinggangmeisu, and the component B is Tea Saponin.
Preferably, the composition is (3-10) by weight ratio：The component A and component B compositions of (3-8).
Preferably, the composition is (5-10) by weight ratio：The component A and component B compositions of (5-8).
The present invention also provides another Pesticidal combination, the composition is (1-20) by weight ratio：The component of (1-10)
A and component B compositions, the component A are made of jinggangmeisu and Pseuomonas denitrifican, the jinggangmeisu and Pseuomonas denitrifican
Weight ratio be 3:1, the component B are Tea Saponin.
The present invention also provides a kind of preparations for desinsection, including above-mentioned composition, and it is total that the composition accounts for the preparation
The percentage of weight is 1-30%, and the preparation further includes at least two in solvent, auxiliary agent, carrier.
Preferably, the percentage that the solvent accounts for the total formulation weight is 1-50%, and it is total that the auxiliary agent accounts for the preparation
The percentage of weight is 1-10%, and the percentage that the carrier accounts for the total formulation weight is 1-40%.
Preferably, the solvent be selected from glycerine, absolute ethyl alcohol, ethylene glycol, pure water it is one or more.
Preferably, the auxiliary agent be selected from fatty alcohol polyoxyethylene ether, alkyl glycosides, fatty alkanol amide, sodium lignin sulfonate,
It is one or more in alkyl naphthalene sodium sulfate salt, succinic acid Diisooctyl sodium sulfonate.
Preferably, the carrier be selected from trisodium citrate, anhydrous magnesium sulfate, potassium dihydrogen phosphate, cornstarch, diatomite,
It is one or more in kaolin.
The present invention also provides a kind of application of above-mentioned composition and preparation on killing diamondback moth, aphid.
Composition of the present invention is all from commercially available, is existing commodity in the prior art.
Jinggangmycin A is validoxylamine by the denitrogenation vacation unit cell enzyme enzymolysis contained by Pseuomonas denitrifican in soil
A, Validoxylamine are the active principles for inhibiting trehalose, can result in insect death.But Validoxylamine is to insect
Epidermis penetration capacity it is poor, it is difficult to reach effect.And Tea Saponin is a kind of outstanding surfactant, is effectively reduced liquid
In insect or the surface tension of plant surface, Validoxylamine is promoted to enter in insect bodies, grabs trehalase inhibitor,
Lead to insect death.
Tea Saponin can destroy insect red blood cell after epidermis enters in insect bodies simultaneously, generate haemolysis.Tea Saponin into
After entering polypide, the membrane passage containing cholesterol is caused to change, destroys cell membrane, and then cytoplasm is caused to exosmose, finally
Make entire erythrocytorrhexis.
Jinggangmeisu is combined with Tea Saponin, is first turned off the energy supply of insect, destroys insect red blood cell.Secondly well ridge is mould
Element is rapid efficiently to enter in insect bodies under the surface-active action of Tea Saponin, improves the targeting to insect, and the two rises
To synergistic effect.And the half-life period of jinggangmeisu in the soil is 3-4 days, and Tea Saponin, which is added, to be rapidly reached
Target simultaneously gives full play to drug effect, and effect on environment is small, not will produce resistance.Last jinggangmeisu may additionally facilitate actinomyces in soil
Growth, actinomyces can also generate various enzyme preparations (protease, amylase and cellulase etc.), vitamin (B12) and organic
Acid etc. stimulates plant growth.
Jinggangmeisu is combined with de- single pseudomonad and is used as component A by another composition provided by the invention, and Tea Saponin is made
It combines for component B, after being exogenously added de- single pseudomonad, can accelerate with the Pseuomonas denitrifican collective effect in soil
The degradation of jinggangmeisu, accelerated degradation product reach target position and play drug effect, the desinsection effect of the composition further improved
It answers, raising acts on yield, promotes object growth.
Specific implementation mode
It is right With reference to embodiment in order to make those skilled in the art more fully understand the technical solution of invention
The present invention is described in further detail.
Diamondback moth is lepidoptera diamond-back moth section, Plutellaxylostella (Linnaeus)；Alias：Small blueness worm, pointed at both ends.
The worldwide pest that migrates, wild cabbage of mainly causing harm, violet cabbage, broccoli, a kind of sedge dish, leaf mustard, cauliflower, Chinese cabbage, rape, radish etc. ten
Zi Hua sections plant.Harm characteristics are first th instar larvae mesophyll, leave epidermis, form spot transparent one by one in dish leaf, 3~
Dish leaf can be eaten into hole and incised by 4 instar larvaes, and full leaf, which is eaten, when serious reticulates.Often lobus cardiacus is concentrated to cause harm in seedling stage, influenced
Packet.It is reserving seed for planting in strain, is endangering tender stem, young pod and seed.
Aphid is commonly called as aphid or aphid, is under the jurisdiction of Semiptera (being Homoptera Hemiptera originally), including adelgid Superfamily
Adelgoidea and Aphidoidea Aphidoidea.Aphid is mainly distributed on Northern Hemisphere Temperate Region in China and subtropical zone, torrid zone ground
Area's distribution is seldom.About more than 4700 plant known to the world, about 1100 kinds of distribution in China.4~5 rake of fore wing, is born in feeler Section 6
The sensation circle of base portion and whip portion intersection is known as " nascent sensation circle ", and that is born in that remaining respectively saves cries " secondary sensation circle ".Aphid is
Polymorphic insect, it is of the same race to have aptery and have wing, there is wing individual to have simple eye, aptery individual is without simple eye.Having the individual 2 pairs of wings of wing, fore wing is big,
Hind wing is small, fore wing nearby edge have 1 merged by longitudinal vein made of thick arteries and veins, there is pterostigma in end.6th uromere back side has 1 pair of cornical, abdomen
There is 1 cauda in portion end.Its medium and small Aphis, black back of the body Aphis and no Aphis are Chinese Endemic Genera.
Above-mentioned pest crop of causing harm not only shows the positions such as sucking plant branches and leaves, tender stem, fruit, can also transmitted virus,
Pest and disease damage is caused to break out.
Organic chemical pesticides prevent pest and disease damage for the mankind and provide strong weapon at present, and evil is being killed in the use of pesticide
Also lead to the far super pest of the susceptibility of the hostile pesticide of drastically reduction or even more day of natural enemy quantity itself, targeting while worm
Property low cause insecticidal effect unsatisfactory.And insecticide is used alone, resistance problem is serious, and Pesticide use is to water body
It is big with soil environment harm, the growth of crop is also inhibited while desinsection, and damage is also resulted in human and environment.
The present invention provides a kind of composition for desinsection, and the composition is (1-20) by weight ratio：The group of (1-10)
It is jinggangmeisu to divide A and component B compositions, the component A, and the component B is Tea Saponin.Preferably, the composition is by weight
Than for (3-10)：The component A and component B compositions of (3-8).Preferably, the composition is (5-10) by weight ratio：(5-8's)
Component A and component B compositions.
The present invention also provides another Pesticidal combination, the composition is (1-20) by weight ratio：The component of (1-10)
A and component B compositions, the component A are made of jinggangmeisu and Pseuomonas denitrifican, the jinggangmeisu and Pseuomonas denitrifican
Weight ratio be 3:1, the component B are Tea Saponin.
The present invention also provides a kind of preparations for desinsection, including above-mentioned composition, and it is total that the composition accounts for the preparation
The percentage of weight is 1-30%, further includes at least two in solvent, auxiliary agent, carrier.
Preferably, the percentage that the solvent accounts for the total formulation weight is 1-50%, and it is total that the auxiliary agent accounts for the preparation
The percentage of weight is 1-10%, and the percentage that the carrier accounts for the total formulation weight is 1-40%.
Preferably, the solvent be selected from glycerine, absolute ethyl alcohol, ethylene glycol, pure water it is one or more.
Preferably, the auxiliary agent be selected from fatty alcohol polyoxyethylene ether, alkyl glycosides, fatty alkanol amide, sodium lignin sulfonate,
It is one or more in alkyl naphthalene sodium sulfate salt, succinic acid Diisooctyl sodium sulfonate.
Preferably, the carrier be selected from trisodium citrate, anhydrous magnesium sulfate, potassium dihydrogen phosphate, cornstarch, diatomite,
It is one or more in kaolin.
The present invention also provides a kind of application of above-mentioned composition and preparation on killing diamondback moth, aphid.
Jinggangmeisu is the microbial pesticide that the success of first, China is developed, and it is most wide to have become China's usable floor area at present,
The minimum pesticide of mu cost.Jinggangmeisu can be decomposed into Validoxylamine and β-D by the Pseuomonas denitrifican in soil
Glucose, and Validoxylamine is the trehalase inhibitor of insect, and trehalose is the insect flight energy.Trehalose
Glucose can be discharged, the effect of the flight energy is provided, and this specific function of trehalose must be in the direct of trehalase
It is just completed under effect.It is by fighting for seaweed that Validoxylamine has stronger affinity, inhibiting effect with trehalase
For carbohydrase come what is realized, Validoxylamine can block the decomposition of trehalose, and insect is made to lose flight performance, cause insect dead
It dies.Although Validoxylamine is very high to the inhibitory activity of in vitro trehalase, the sterilization of compound live body, a desinsection
The quality of effect not fully depends on inhibiting the active height of unorganized ferment, it is often more important that can compound reach " enzyme target
Mark ".And the mould subunit A in well ridge is the water soluble compound of polyhydroxy, it is difficult to which penetrating insect body wall reaches " enzyme target ", is only passed through
Injecting drug use, insecticidal effect can just be shown by directly kidnapping enzyme target.
Tea Saponin sterling is the fine columnar crystal of colorless and odorless, and the Tea Saponin containing impurity is general brown to EGG YOLK SHORTCAKE
Powdered, purity is higher, and color is lighter, and Tea Saponin itself is a kind of nonionic surface active agent, can reduce surface tension.Tea
Saponin has pest certain direct poisoning ability, reaches 83.9% to low age cabbage butterfly pretty young woman's larva killing effect, to vegetable powder
Butterfly, diamondback moth, prodenia litura play the role of stronger Antifeedant Effects and inhibit growth and development, to cabbage caterpillar have certain stomach toxicity and
Stronger deterrent effect.
Secondly there is Tea Saponin itself bacteriostatic activity, antifungal mechanism to be mainly shown as：Inhibit the synthesis of protein, inhibits
Mycelia grows and spore germination activity, improves the superoxide dismutase activity of thalline, promotes O in somatic cells2-It is oxidized to
H2O2, H2O2Accumulation can damage cells interior metabolic component.It can also reduce endobacillary peroxidase activity, make
The oxidation of the reducing substances such as phenols, aromatic amine and ascorbic acid in somatic cells is suppressed, and influences the normal generation of thalline
It thanks.
When Tea Saponin is combined with jinggangmeisu, it can be substantially reduced medical liquid surface tension, reduce drop connecing in target body surface
Feeler improves medicament effective precipitation on target body, is allowed to be conducive to moisten spread on target body biology, to improve drug effect, also prolong
The generation of resistance is delayed.Animal erythrocyte can also be destroyed simultaneously, stimulated plant growth, improved target-oriented drug and drug effect
While, have the function that increase production increment.
It, can be false single with the denitrogenation in soil after another composition provided by the invention is exogenously added de- single pseudomonad
Born of the same parents' bacterium collective effect, accelerates the degradation of jinggangmeisu to obtain Validoxylamine, and accelerated degradation product reaches target position and plays
Drug effect, the insecticidal effect of the composition further improved, raising act on yield, promote object growth.
Above-mentioned elaborating for the present invention is the embodiment of the present invention below.
Embodiment one
Jinggangmeisu：1%
Tea Saponin：1%
Pseuomonas denitrifican：0.3%
Fatty alcohol polyoxyethylene ether：3.0%
Pure water：Complement to 100%
(1) pure water prepares kettle according to stoichiometry, input in being formulated；
(2) jinggangmeisu in formula, Tea Saponin input are prepared into kettle stirring and dissolving；
(3) fatty alcohol polyoxyethylene ether mixing time is added 30-60 minutes.
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 1：
One preparation stability test result of embodiment
Embodiment two
Jinggangmeisu：3%
Tea Saponin：1%
Pseuomonas denitrifican:1.0%
Alkyl glycosides：3.0%
Pure water：Complement to 100%
(1) pure water prepares kettle according to stoichiometry, input in being formulated；
(2) jinggangmeisu in formula, Tea Saponin input are prepared into kettle stirring and dissolving；
(3) fatty alcohol polyoxyethylene ether mixing time is added 30-60 minutes.
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 2：
Two preparation stability test result of embodiment
Embodiment three
Jinggangmeisu：5%
Tea Saponin：5%
Absolute ethyl alcohol：20%
Alkyl glycosides：3.0%
Pure water：Complement to 100%
(1) pure water prepares kettle according to stoichiometry, input in being formulated；
(2) jinggangmeisu in formula, Tea Saponin input are prepared into kettle stirring and dissolving；
(3) fatty alcohol polyoxyethylene ether mixing time is added 30-60 minutes.
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 3：
Three preparation stability test result of embodiment
Example IV
Jinggangmeisu：8%
Tea Saponin：5%
Glycerine：20%
Fatty alkanol amide：5%
Pure water：Complement to 100%
(1) pure water prepares kettle according to stoichiometry, input in being formulated；
(2) jinggangmeisu in formula, Tea Saponin input are prepared into kettle stirring and dissolving；
(3) fatty alcohol polyoxyethylene ether mixing time is added 30-60 minutes.
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 4:
Example IV preparation stability test result
Embodiment five
Jinggangmeisu：10%
Tea Saponin：10%
Pseuomonas denitrifican：3.0%
Trisodium citrate:20%
Anhydrous magnesium sulfate：20%
Potassium dihydrogen phosphate complements to 100%
(1) by each raw material in formula according to stoichiometry, input mixing vessel in carry out premixing 10-15 minutes；
(2) material after premixing is crushed；
(3) material after crushing is waken up with a start and is remixed, incorporation time 10-15 minutes.
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 5:
Five preparation stability test result of embodiment
Embodiment six
Jinggangmeisu：20%
Tea Saponin：5%
Sodium lignin sulfonate 8.0%
Alkyl naphthalene sodium sulfate salt 2.0%
Succinic acid Diisooctyl sodium sulfonate 2.0%
Cornstarch：Complement to 100%
Preparation method is：
(1) by each raw material in formula according to stoichiometry, input mixing vessel in carry out premixing 10-15 minutes；
(2) material after premixing is subjected to air-flow crushing；
(3) material after crushing is remixed, incorporation time 10-15 minutes.
(4) water of the weight that adds materials 10% or so is mediated, is granulated, is dried, and water dispersible granules finished product is formed
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 6:
Six preparation stability test result of embodiment
Embodiment seven
Jinggangmeisu：1%
Tea Saponin：10%
Pseuomonas denitrifican：0.3%
Sodium lignin sulfonate 8.0%
Alkyl naphthalene sodium sulfate salt, 2.0%
Succinic acid Diisooctyl sodium sulfonate 2.0%
Diatomite：Complement to 100%
(1) by each raw material in formula according to stoichiometry, input mixing vessel in carry out premixing 10-15 minutes；
(2) material after premixing is subjected to air-flow crushing；
(3) material after crushing is remixed, incorporation time 10-15 minutes.
With reference to NY/T 1427-2007 pesticide storage at normal temperature stability test general rules, stability is carried out to aforesaid liquid preparation
Experiment, test result such as table 7:
Seven preparation stability test result of embodiment
Embodiment eight
Indoor bioassay is tested
1, experimental subjects：Diamondback moth
Diamondback moth is lepidoptera diamond-back moth section, Plutella xylostella (Linnaeus)；Alias：Small blueness worm, both ends
Point.The peak period that causes harm in whole year is different due to from different places, and northeast, North China are to cause harm the 5-6 months and the 8-9 months seriously, and spring
Overweight autumn.Then cause harm the 7-8 months in Xinjiang most heavy.With the 3-6 months and the 8-11 months it is occurance peak in south, and autumn overweights the spring
Season.Adult is hidden by day and come out at night, and daytime is hidden in plant clump more, and post sunset comes into play.There is phototaxis, to be to flutter lamp when 19-23
Peak period.It can mate quickly after adult eclosion, female moth that night of mating lays eggs.Female adult lasts a long time, oviposition duration
Long, the especially winter generation adult egg-laying season can be longer than next-generation larval phase.
Diamondback moth has typical insect evolution advantage:1 body is small, can survive as long as having a small amount of food, is easy to hide enemy
Evil.2 life cycles are short, feeding wild cabbage, when temperature 28-30 is spent, as long as it is most 10 days fast to complete a generation.3 fertilities are strong, per female
Egg laying amount average 220, ovum dissipates production.4 winter generation adult egg-laying seasons up to 90 days, resulted in serious generation overlap in this way, prevention
It is difficult.5 annidations are strong, and winter can hold out through short-term subzero 15 degree of severe cold, can also feeding activity in the environment of -1.4 degree.
Summer can worry along 35 degree or more heats, and the only heavy rain in summer can kill them in large quantities.6 drug resistances are strong, due to using all the year round
Chemical pesticide control, largely kills natural enemy, and diamondback moth is caused harm with increasing intensity daily, and is produced quickly to all kinds of chemical pesticides high
Horizontal resistance, the 90's of last century year, many places were rampant in face of diamondback moth, pasted medical help.Since occurring area is big, cause harm
Time is long, and prevention is difficult, and diamondback moth gradually replaces cabbage caterpillar and becomes No. 1 pest of vegetables.
2, the toxicity test of jinggangmeisu and Tea Saponin to diamondback moth.,
3, aggrieved using the phototaxis night of diamondback moth, it props nylon wire and attracts diamondback moth, contained in each bag
100, and fasten sack.
4, data statistic analysis：It is analyzed using DPS6.55 (Duncan) statistical software.By jinggangmeisu and Tea Saponin
The solution for being configured to 1000mg/L respectively is configured to the mixed solution of different proportion, and multiple in jinggangmeisu single dose or jinggangmeisu
Jinggangmeisu and Pseuomonas denitrifican concentration ratio are 3 in preparaton:1, it is compareed with clear water.It is each to locate using spraying method
Reason 3 repetitions of setting, spray once after 7 days, then calculate the death rate and Regeneration rate again.It is calculated extremely according to test data
Die rate C1, corrected mortality C2.Calculation formula is as follows：
The death rate, %=death toll/processing number * 100
Corrected mortality, %=(the processing death rate-control death rate)/(1- compares the death rate) * 100
Theoretical death rate, %=X+Y-XY/100
X is the actual of Tea Saponin under concentration to use actual, Y using jinggangmeisu under concentration.Compare
Theoretical preventive effect and practical preventive effect, evaluate the synergistic effect of composition, are more than theoretical preventive effect as synergistic effect using practical preventive effect.
Merit-order method, the most common natural enemy of diamondback moth.Frontline is good but small to natural enemy to the killing effect of diamondback moth
The killing rate 12h of Parasitoid Cotesia plutellae reaches 100%.
4, measurement result
Table 8, single medicine or composition are applied to the indoor virulence test result of diamondback moth
The natural enemy of diamondback moth has parasitic wasp and spider, and the parasitic wasp for such as preying on diamondback moth has merit-order method, diamondback moth
Initial bow, Diadromus collaris etc., the spider for preying on diamondback moth have 12 kinds, mainly there is carnivorism ditch tumor spider, erigonidium graminicolum, eight spots
Theridiid, near-ring line tarantula, Misumenops tricuspidatus etc..Rice-pudding tube spinner is up to the per day predatory number of 2-3 instar larvae of diamondback moth
17.6, followed by near-ring line tarantula, eight spot theridiid attacks are minimum, and per day predatory number is only 2.4.
In order to verify safety of the jinggangmeisu to diamondback moth natural enemy, diamondback moth spot trap merit-order method,
Each 300 of near-ring line tarantula, the solution that jinggangmeisu and Tea Saponin are configured to 1000mg/L is configured to the mixing of different proportion
Solution, and jinggangmeisu and Pseuomonas denitrifican a concentration of 3 in jinggangmeisu single dose or composite jinggangmycin preparation:1,5%
Fluorine worm cyanogen suspending agent is configured to 60mg/L solution, is compareed with clear water.Using spraying method, 3 repetitions of each processing setting,
It is sprayed again after 7 days once, then calculates the death rate.
Table 9, single medicine or composition are applied to the indoor virulence test result of diamondback moth natural enemy
It after the above results show that jinggangmeisu is combined with Pseuomonas denitrifican, then combines with Tea Saponin, diamondback moth is killed
The effect of going out plays the role of synergy, and killing rate is also greatly reduced the toxicity of diamondback moth natural enemy up to 100%.
Embodiment nine
Indoor bioassay is tested
1, experimental subjects：Aphid
Aphid --- the most fast insect of breeding.Aphid is commonly called as aphid or aphid etc., and it (was Homoptera originally to be under the jurisdiction of Semiptera
), including adelgid Superfamily Adelgoidea and Aphidoidea Aphidoidea Hemiptera.Aphid is mainly distributed on Northern Hemisphere temperate zone
Area and subtropical zone, torrid areas distribution are seldom.About more than 4700 plant known to the world, about 1100 kinds of distribution in China.Fore wing 4~
5 rakes, are born in feeler Section 6 base portion and the sensation circle of whip portion intersection is known as " nascent sensation circle ", are born in what remaining was respectively saved
It cries " secondary sensation circle ".Aphid is polymorphic insect, of the same race to have aptery and have wing, has wing individual to have simple eye, and aptery individual is without simple eye.
Have the individual 2 pairs of wings of wing, fore wing is big, and hind wing is small, fore wing nearby edge have 1 merged by longitudinal vein made of thick arteries and veins, there is pterostigma in end.The
6 uromere back sides have 1 pair of cornical, abdomen end to have 1 cauda.Its medium and small Aphis, black back of the body Aphis and no Aphis are Chinese Endemic Genera.
Pest crop of causing harm not only shows the positions such as sucking plant branches and leaves, tender stem, fruit, can also transmitted virus, cause
Pest and disease damage is broken out.
2, the toxicity test of jinggangmeisu and Tea Saponin to aphid.,
3, it aggrieved props nylon wire and attracts aphid, contain 100 in each bag, and fasten sack.
4, data statistic analysis：It is analyzed using DPS6.55 (Duncan) statistical software.By jinggangmeisu and Tea Saponin
The solution for being configured to 1000mg/L is configured to the mixed solution of different proportion, is compareed with clear water.Using spraying method, each
Processing 3 repetitions of setting, spray once after 7 days, then calculate the death rate and corrected mortality again.It is calculated according to test data
Death rate C1, corrected mortality C2.
The death rate, %=death toll/processing number * 100
Corrected mortality, %=(the processing death rate-control death rate)/(1- compares the death rate) * 100
Theoretical death rate, %=X+Y-XY/100
X is the actual of Tea Saponin under concentration to use actual, Y using jinggangmeisu under concentration.Compare
Theoretical preventive effect and practical preventive effect, evaluate the synergistic effect of composition, are more than theoretical preventive effect as synergistic effect using practical preventive effect.
5, measurement result
Table 10, single medicine or composition are applied to the laboratory test results of aphid
Aphid is one of most destructive pest on the earth, natural enemy have ladybug, wasp fly, parasitic wasp, food aphid cecidomyiia,
Thomisid, Chrysopa.
Predaceous Coccinellids are mostly food with aphid, and many ladybugs only live in specific habitat, are only limitted to specific life sometimes
On a few plant in border, leaving away when prey tails off in habitat, he goes out search of food.
Wasp fly is the effective natural enemies of aphid, scale insect, aleyrodid, leafhopper, thrips, Lepidoptera small grub etc., is Diptera
Hover fly insect, with larva prey on aphid and title.
Parasitic wasp refers to the various insects that Pteromalidae, Ichneumonidae, Chalcididae etc. in Hymenoptera apocrita lean on parasitic life,
There are many type, parasitize the different developmental phases of host respectively, common are chalcid fly, ichneumon wasp, cocoon bee, trichogramma, nibble chalcid fly etc..
Food aphid cecidomyiia is Diptera Cecidomyiidae insect, is distributed in the areas such as Hubei, host insect is cotten aphid, with the larva that cocoons
It is overwintering under table soil around the host plant of aphid, pupate between 3~April of next year, emergence post-coitum in the weeds that have aphid and
It lays eggs and breeds on the early springs host such as rose of Sharon.
In order to verify safety of the jinggangmycin A to natural enemy of aphid, ladybug, wasp fly each 150 are traped in aphid spot
Only, the solution that jinggangmeisu and Tea Saponin are configured to 1000mg/L is configured to the mixed solution of different proportion, 350g/L pyrrole worms
Quinoline suspending agent is configured to 100mg/L solution, is compareed with clear water.Using spraying method, 3 repetitions of each processing setting, 7 days
It sprays again afterwards once, then calculates the death rate.
Table 11, single medicine or composition are applied to the laboratory test results of natural enemy of aphid
The above results show that jinggangmeisu is combined with Tea Saponin, play the role of synergy to the killing effect of aphid, kill
Rate go out up to 100%, while being also greatly reduced the toxicity of natural enemy of aphid.
Embodiment ten
The test of pesticide effectiveness
1, subjects:Wild cabbage diamondback moth
2, test period：Mid-April
3, climate condition：15-20 DEG C, 3-6 grades of wind speed, humidity 60-75%
4, test method：It selects diamondback moth that uniform and Relatively centralized occurs experimental field, jinggangmeisu, Tea Saponin is distinguished
It is configured to 1000mg/L concentration, is compounded according to different proportion, and in jinggangmeisu single dose or composite jinggangmycin preparation
Jinggangmeisu and Pseuomonas denitrifican a concentration of 3:1.Using spraying method, 3 repetitions of each processing setting are sprayed again after 7 days
Once, after 10 days, record Revision insect recluced rate calculates preventive effect, the results are shown in Table 12
Revision insect recluced rate %=(insect population death toll+insect population survival number-insect population survival number)/(insect population death toll+insect population survival
Number) * 100
Preventive effect (%)=[1- (borer population living before borer population × check plot medicine of living after treatment region medicine)/(borer population of living before treatment region medicine ×
Borer population living after the medicine of check plot)] × 100
Theoretical preventive effect=single dose A preventive effects+single dose B preventive effects-preventive effect/100 single dose A preventive effect * single dose B
Meanwhile Cabbage Quality is evaluated, the influence that composition of the present invention grows wild cabbage is judged, with wild cabbage egg
White matter, VC and potassium content are evaluation index, the results are shown in Table 13.
12 each single dose of table or composition are applied to the test of pesticide effectiveness result of wild cabbage diamondback moth prevention
13 each single dose of table or composition are applied to the test result of most Cabbage Quality influence after diamondback moth
It after the above results show that jinggangmeisu is combined with de- single pseudomonad, then combines with Tea Saponin, not only increases pair
The preventive effect of diamondback moth, highest preventive effect is up to 98.14%, moreover it is possible to improve the quality of wild cabbage.
Embodiment 11
The test of pesticide effectiveness
1, subjects：Pea aphid
2, test period：Early March
3, climate condition：10-15 DEG C, 3-6 grades of wind speed, humidity 60-75%
Test method：It selects aphid that uniform and Relatively centralized occurs experimental field, jinggangmeisu, Tea Saponin is prepared respectively
At 1000mg/L concentration, compounded according to different proportion.Using spraying method, 3 repetitions of each processing setting, after 7 days
It sprays again once, after 10 days, record Revision insect recluced rate calculates preventive effect, the results are shown in Table 14
Revision insect recluced rate %=(insect population death toll+insect population survival number-insect population survival number)/(insect population death toll+insect population survival
Number) * 100
Preventive effect (%)=[1- (borer population living before borer population × check plot medicine of living after treatment region medicine)/(borer population of living before treatment region medicine ×
Borer population living after the medicine of check plot)] × 100
Theoretical preventive effect=single dose A preventive effects+single dose B preventive effects-preventive effect/100 single dose A preventive effect * single dose B
Meanwhile pea quality is evaluated, the influence that composition of the present invention grows pea is judged, with pea seedlings
Length, beanpod number, protein content, VC contents and VA contents are evaluation index, the results are shown in Table 15,16.
Table 14, each single dose or composition are applied to the test result of drug effect examination pea aphid
Pea happiness freezing moist climate, resists cold, and thermo-labile, seedling is resistant to 5 DEG C of low temperature, 12~16 DEG C of growth period thermophilic, knot
15~20 DEG C of more than 25 DEG C rates of fertilization of pod phase thermophilic are low, bear pods less, low output.
Pea is long-day plant, and the breeding time of most kinds is shorter than south in north performance.Southern kind is moved northward ahead of time
Bloom bear pods, this is shortened with north spring sowing in the overwintering Seedling Stage in south, therefore in the north, the breeding time of pea, early seasonal strain 65
~75 days, middle seasonal strain 75~100 days, seasonal strain 100~185 days.
Though pea soil is required it is not tight, on well-drained husky earth or it is newly-ploughed can transplant, with it is loose containing
Higher neutral (pH 6.0~7.0) soil of machine matter is advisable, and the development of advantageous emergence and rhizobium, soil aciditiy is less than pH 5.5
When easily occur disease and reduce Pod Bearing Percentage, lime improvement should be affixed by.Pea root system is deep, slightly drought-enduring without moisture-proof, sowing or seedling
Impeded drainage easily rotten, Drought Stress at Flowering insemination is bad, is easy to form empty pod or not plump pod.
Enzyme rich in nitrosamine in vitamin C and energy decomposer in the tender leaf of pea pods and bean seedlings, can decompose nitrous
Amine has the function of anti-cancer and cancer-preventing.Pea and general vegetables are different, and contained only wooden fork acid, gibberellin and plant coagulate element etc.
Substance has antibacterial anti-inflammatory, enhances the function of metabolism.Contain the dietary fiber compared with horn of plenty in garden pea and bean seedlings,
Constipation can be prevented, there is gut purge effect.
Table 15, each single dose or composition are applied to after pea aphid to the test result of pea yield effect
16 each single dose of table or composition are applied to the test result to pea qualitative effects after pea aphid
The above results show that jinggangmeisu is combined with Tea Saponin, can not only improve the preventive effect to pea aphid, highest preventive effect
Up to 93.73%, while pea yield can also be improved, improve pea quality.
It the above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
The limitation of the present invention, protection scope of the present invention should be subject to claim limited range.For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
Protection scope of the present invention is also should be regarded as into retouching.
Claims (10)
1. a kind of composition for desinsection, the composition is (1-20) by weight ratio：The component A and component B groups of (1-10)
At the component A is jinggangmeisu, and the component B is Tea Saponin.
2. the composition according to claim 1 for desinsection, which is characterized in that the composition is (3- by weight ratio
10)：The component A and component B compositions of (3-8).
3. the composition according to claim 2 for desinsection, which is characterized in that the composition is (5- by weight ratio
10)：The component A and component B compositions of (5-8).
4. the composition according to claim 1 for desinsection, which is characterized in that the component A is by jinggangmeisu and takes off
Nitrogen pseudomonad forms, and the weight ratio of the jinggangmeisu and Pseuomonas denitrifican is 3:1.
5. a kind of preparation for desinsection, including claim 1-4 any one of them compositions, which is characterized in that described group
It is 1-30% to close object and account for the percentage of the total formulation weight, and the preparation further includes at least two in solvent, auxiliary agent, carrier
Kind.
6. preparation according to claim 5, which is characterized in that the percentage that the solvent accounts for the total formulation weight is
1-50%, the percentage that the auxiliary agent accounts for the total formulation weight is 1-10%, and the carrier accounts for the total formulation weight
Percentage is 1-40%.
7. preparation according to claim 6, which is characterized in that the solvent be selected from glycerine, absolute ethyl alcohol, ethylene glycol,
Pure water it is one or more.
8. preparation according to claim 6, which is characterized in that the auxiliary agent is selected from fatty alcohol polyoxyethylene ether, alkyl sugar
It is one or more in glycosides, fatty alkanol amide, sodium lignin sulfonate, alkyl naphthalene sodium sulfate salt, succinic acid Diisooctyl sodium sulfonate.
9. preparation according to claim 6, which is characterized in that the carrier is selected from trisodium citrate, anhydrous magnesium sulfate, phosphorus
It is one or more in acid dihydride potassium, cornstarch, diatomite, kaolin.
10. any one of any one of the claim 1-4 compositions and claim 5-9 preparations kill diamondback moth,
Application on aphid.
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| CN111248203A (en) * | 2020-03-04 | 2020-06-09 | 天津农学院 | Application of tea saponin in improving crop yield and method for improving crop yield by using tea saponin |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430943A (en) * | 2013-07-05 | 2013-12-11 | 陕西省蒲城美尔果农化有限责任公司 | Synergistic kasugamycin composition containing tea saponin |
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2018
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430943A (en) * | 2013-07-05 | 2013-12-11 | 陕西省蒲城美尔果农化有限责任公司 | Synergistic kasugamycin composition containing tea saponin |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111248203A (en) * | 2020-03-04 | 2020-06-09 | 天津农学院 | Application of tea saponin in improving crop yield and method for improving crop yield by using tea saponin |
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