CN101984828A - Compound pesticide - Google Patents
Compound pesticide Download PDFInfo
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- CN101984828A CN101984828A CN2010105246175A CN201010524617A CN101984828A CN 101984828 A CN101984828 A CN 101984828A CN 2010105246175 A CN2010105246175 A CN 2010105246175A CN 201010524617 A CN201010524617 A CN 201010524617A CN 101984828 A CN101984828 A CN 101984828A
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- green muscardine
- compounded pesticides
- muscardine fungus
- nimbin
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Abstract
The invention discloses a compound pesticide, which is mainly prepared from the following raw materials in percentage by weight: 5-20% of azadirachtin and metarrhizium, 8-12% of emulsifier, 3-7% of stabilizer, 2-8% of synergist, 0.1-1.0% of antioxidant, 10-18% of cosolvent and 50-60% of organic solvent. The compound pesticide is environment-friendly, has high compatibility and obvious synergistic effect, and can obviously improve the insecticidal toxicity of a single dosage and reduce the use of high-persistent chemical pesticide.
Description
Technical field
The present invention relates to a kind of compound biotic insecticide, be specifically related to a kind of green muscardine fungus and nimbin compounded pesticides.
Background technology
Green muscardine fungus is a kind of insect pathogenic fungus of broad spectrum activity, is widely used in multiple farming, woods, herds, the control of sanitary insect pest.Research and the history in existing more than 100 year of application green muscardine fungus control agriculture and forestry injurious insect.Over nearly 20 years, research is all over the world used the green muscardine fungus pest control and is reached kind more than 300, has 100 various pests to carry out field controling test approximately, and from the basic field that scale of preventing and treating and research relate to, green muscardine fungus has become the fungus insecticide that is only second to white muscardine fungi.Green muscardine fungus can be used for the soil insect in nursery in forestry, the trees termite, the biological control of trunk borer and defoliator, on agricultural, can be used for the grub control of the soil insect, paddy rice froghopper, peanut, sugarcane etc. of pollution-free vegetable, can be used for the biological control of herbage locust and soil insect in the livestock breeding.But, on using, be subjected to certain limitation because of fungus insecticide has control efficiency instability, the slow relative more high shortcoming with cost of desinsection speed.
Nimbin is as a kind of botanical pesticide efficiently, it is the insecticide that at present universally acknowledged wide spectrum, efficient, low toxicity, easily degraded, noresidue, target pest are difficult for developing immunity to drugs, nearly all plant insect is all had repelling and killing efficacy, and people and animals and surrounding environment are not had any pollution.To target pest have food refusal, repellent, inhibition growth, disturb lay eggs, effects such as interference variation, effectively Pest Control causes harm.Proved to various pests tool insecticidal action, can prevent and treat and plant farming, woods, storage and sanitary insect pest more 200 especially insects such as Lepidoptera, coleoptera and diptera are had special efficacy.
Since entering the chemical insect protected epoch, people more and more come by force the dependence of chemical pesticide, the long-term use of some chemical chemical insecticide, increased insect pesticide resistance, polluted environment.Multiple mixed insecticide is arranged in the market, mostly be being mixed between the chemical pesticide of different mechanism of action, its usage amount is big, and insect easily develops immunity to drugs.The long-term use of various chemical medicaments has caused the increase of agricultural land used persticide residue, it is limited to cause multiple product to be concluded the business in the international market, and insect to the drug-fast increase of medicament and medicament to the increase that also causes dosage and medicine frequency that kills and wounds of natural enemy insect, usually cause the generation again of a certain insect.
For the insecticidal rate that improves fungus insecticide, reduce use cost, with fungus insecticide and the composite use of compatibility chemical pesticide, but exist the compatibility medicament in the production to problems such as the influence of fungal spore vigor in composite dose and chemical pesticide environmental pollutions.
Summary of the invention
The object of the invention is to provide a kind of compounded pesticides, this insecticide and environmental friendliness, can enlarge biopesticide insecticidal spectrum, increase insecticidal effect, reduce the use of high residue chemical pesticide.
A kind of compounded pesticides provided by the invention, it is mainly made by the raw material of following weight percentage:
Nimbin and green muscardine fungus 5-20%
Emulsifier 8-12%
Stabilizing agent 3-7%
Synergist 2-8%
Antioxidant 0.1-1.0%
Cosolvent 10-18%
Organic solvent 50-60%.
The weight part ratio of green muscardine fungus of the present invention and nimbin is 25-99: 1.
Wherein, nimbin and entomogenous fungi are compatible, and have different desinsection modes and desinsection object, and the two carries out the compound new compounded pesticides that obtains, and it is general to enlarge desinsection, improve insecticide efficiency.Prepared insecticide has the brand-new mechanism of action that is different from existing insecticide, and insecticidal effect is remarkable, and is especially remarkable at the serious lepidoptera pest toxic action of harm, has eco-friendly characteristics simultaneously.
Green muscardine fungus of the present invention is the conidium that solid fermentation obtains, the high cryptogam that after extracting, obtains, the spore content that its weight metering mode is composite dose of endoconidium total content/pure conidial powder (spore/g).
Emulsifier of the present invention is preferably tween, many sorbitol esters, sodium alkyl sulfate, NaLS or alkylbenzenesulfonate.
Stabilizing agent of the present invention is preferably epoxidized soybean oil or epoxidized linseed.
Synergist of the present invention is preferably Butacide, chlorination fat, its ester of carbonic acid alkane or sulfoxide.
Antioxidant of the present invention is preferably one or more in butyl alkyl anisole, dibutyl toluene, n-propyl gallate and the dilauryl thiodipropionate.
Cosolvent of the present invention is preferably one or more in isoamyl alcohol, phenol, ethyl acetate and the dimethyl sulfoxide (DMSO).
Organic solvent of the present invention is preferably dimethylbenzene.
The preparation method of above-mentioned compounded pesticides is as follows: take by weighing high cryptogam of green muscardine fungus and nimbin raw medicine by above-mentioned metering ratio, earlier nimbin raw medicine is dissolved in the solvent that contains part antioxidant and stabilizing agent, quantitatively add the high cryptogam of green muscardine fungus, emulsifier, synergist and cosolvent again, airtight stirring 10-15min, add residue antioxidant, stabilizing agent and organic solvent again, and be adjusted to aimed concn, and continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Compared with prior art, the present invention has following advantage:
1.. nimbin and muscardine spore have favorable compatibility, and synergistic effect is obvious, can obviously improve the insecticidal toxicity of each single agent;
2.. production technology is simple, can reduce the usage amount of agricultural chemicals significantly, delays the pesticide resistance of insect, and insect is easy to form epidemic disease, and effect is obvious in the non-environmental pollution control of insect and lasting integrated control;
3.. to be that host is composite by the agent of two kinds of Biocidal lists form composite insecticide, the compatibility good, extensive use with the environment tool to agricultural production and income, development environmental protection agricultural, improve the ecological environment, improve level of human health, realize that the strategy of sustainable development is significant.
Embodiment
The present invention is further illustrated below in conjunction with specific embodiment, and following reagent and raw material are commercially available if no special instructions.
Embodiment 1
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 10%, and the weight proportion of each raw material is as follows:
Nimbin 0.2g
Green muscardine fungus 9.8g (15,000,000,000 spores/g, down together)
Emulsifier 10g
Stabilizing agent 5g
Synergist 5g
Antioxidant 0.5g
Cosolvent 14.5g
Organic solvent 55g.
Wherein the high cryptogam of green muscardine fungus can obtain (down together) in the following way: the green muscardine fungus bombys batryticatus with gathering, through indoor separating and preserving,, filter out the green muscardine fungus bacterial strain that several forestry pests is had high virulence through Toxicity Determination, and be used to produce the muscardine spore powder.Adopt liquid-solid biphasic fermentation to cultivate, utilize closed fungus insecticide solid fermentation culture bag, the good fermenting green muscardine fungus liquid of inoculated and cultured in solid culture medium (rice), seal cultivation, treat that solid culture medium covers with and open bag behind the mycelia and cultivate, wait to produce spore and dry the negative pressure extracting after fully, get the high cryptogam of green muscardine fungus, perhaps directly buy commercially available muscardine spore powder.
The preparation process of above-mentioned prescription insecticide is: accurately take by weighing high cryptogam of green muscardine fungus and nimbin raw medicine, earlier nimbin raw medicine is dissolved in the solvent that contains part antioxidant butyl alkyl anisole, cosolvent isoamyl alcohol and stabilizing agent epoxidized soybean oil, add the high cryptogam of green muscardine fungus, emulsifier tween, synergist Butacide again, airtight stirring 10-15min, add residue antioxidant, stabilizing agent and organic solvent dimethylbenzene again, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Embodiment 2
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 8%, and the weight proportion of each raw material is as follows:
Nimbin content 0.2g
Green muscardine fungus content 7.8g
Emulsifier 10g
Stabilizing agent 6g
Synergist 6g
Antioxidant 0.5g
Cosolvent 14.5g
Organic solvent 55g.
The preparation process of above-mentioned prescription insecticide suspending agent is: accurately take by weighing high cryptogam of green muscardine fungus and nimbin raw medicine, earlier nimbin raw medicine is dissolved in the solvent that contains part antioxidant n-propyl gallate and stabilizing agent epoxidized soybean oil, add the high cryptogam of green muscardine fungus, the many sorbitol esters of emulsifier, synergist chlorination fat and cosolvent phenol again, airtight stirring 10-15min, add residue antioxidant, stabilizing agent and organic solvent dimethylbenzene again, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Embodiment 3
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 15%, and the weight proportion of each raw material is as follows:
Nimbin content 0.3g
Green muscardine fungus content 14.7g
Emulsifier 8g
Stabilizing agent 4g
Synergist 4g
Antioxidant 0.5g
Cosolvent 13.5g
Organic solvent 55g.
The preparation process of above-mentioned prescription insecticide suspending agent is: accurately take by weighing high cryptogam of green muscardine fungus and nimbin raw medicine, earlier nimbin raw medicine is dissolved in the solvent that contains part antioxidant dilauryl thiodipropionate and stabilizing agent epoxidized linseed, add the high cryptogam of green muscardine fungus, emulsifier sodium alkyl sulfate, synergist sulfoxide and cosolvent ethyl acetate again, airtight stirring 10-15min, add residue antioxidant, stabilizing agent and organic solvent dimethylbenzene again, and be adjusted to aimed concn, continue to stir 10-20min, be prepared into the compounded pesticides of oil-suspending agent type.
Embodiment 4
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 5%, and the weight proportion of each raw material is as follows:
Nimbin 0.19g
Green muscardine fungus 4.81g
Emulsifier 12g
Stabilizing agent 3g
Synergist 8g
Antioxidant 0.1g
Cosolvent 18g
Organic solvent 50g.
Its preparation method is with embodiment 1-3.
Embodiment 5
The preparation weight percentage is (green muscardine fungus+nimbin) suspending agent of 20%, and the weight proportion of each raw material is as follows:
Nimbin 0.4g
Green muscardine fungus 19.6g
Emulsifier 8g
Stabilizing agent 7g
Synergist 2g
Antioxidant 1.0g
Cosolvent 10g
Organic solvent 60g.
Its preparation method is with embodiment 1-3.
The Toxicity Determination of compounded pesticides of the present invention and woodland control efficiency:
One, the Toxicity Determination effect of compounded pesticides
Reagent agent: green muscardine fungus-nimbin suspending agent (the Ma985 bacterial strain of green muscardine fungus for snout moth is had high virulence, green muscardine fungus and nimbin weight ratio are 98: 2, green muscardine fungus content is 10,000,000,000 spores/ml); For trying insect: oranges and tangerines long paper moth 3 instar larvaes of harm Aegiceras corniculatum.Gather the 3-4 paulownia oranges and tangerines long paper moth larvae in age from woodland, indoor feeding 2-3 days, select the active larva of health, put into plastics square box (17cm * 17cm), 3 repetitions of every processing, 10 larvas of every repetition.With 1000 times of institute's dose out powders dilutions, be sprayed to blade surface, 26 ± 1 ℃, raise under 80 ± 1%RH condition, after one day, the blade of being replaced by no medicine continues to raise, and compares with the distilled water that sprays with amount.See examination worm death condition every day, and change fresh blade, observe a week continuously, add up the corrected mortality of each processed group.
Table 1 green muscardine fungus and nimbin mixed toxicity test to oranges and tangerines long paper moth
Two, compounded pesticides woodland control efficiency:
Woodland control test site is selected in high bridge protection station, country Lianjiang county, mangrove forest nature reserve, Zhanjiang carries out, the defoliator oranges and tangerines long paper moth that the control paulownia is taken, and insect mainly is in the middle instar larvae stage, and a small amount of mature larva, pupa and adult are arranged.Investigation insect population radix experimental field before the spray medicine, and gather 20 larvas in contrast.Weight percentage is evenly to be sprayed on leaf surface behind 500 times of 15% the green muscardine funguss-nimbin emulsion dilution, and every mu of consumption 100ml behind the spray medicine 24h, 20 larvas of each treatment region random acquisition, in indoor feeding, observes the polypide death condition.
Table 2 green muscardine fungus and nimbin mixed woodland control oranges and tangerines long paper moth drug effect are analyzed
Annotate: " A " represents adult in the table, and " L " represents larva, and " P " represents pupa, and the adult that sprouts wings in the process of the test all is considered as surviving, and contrast examination worm is four mixing that gather in the district.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included in protection scope of the present invention.
Claims (7)
1. compounded pesticides, it is characterized in that: it is mainly made by the raw material of following weight percentage:
Nimbin and green muscardine fungus 5-20%
Emulsifier 8-12%
Stabilizing agent 3-7%
Synergist 2-8%
Antioxidant 0.1-1.0%
Cosolvent 10-18%
Organic solvent 50-60%.
2. compounded pesticides according to claim 1 is characterized in that: the weight part ratio of described green muscardine fungus and nimbin is 25-99: 1.
3. compounded pesticides according to claim 1 and 2 is characterized in that: described green muscardine fungus is the high cryptogam that the conidium of solid fermentation acquisition obtains after extracting.
4. compounded pesticides according to claim 1 and 2 is characterized in that: described emulsifier is tween, many sorbitol esters, sodium alkyl sulfate, NaLS or alkylbenzenesulfonate.
5. compounded pesticides according to claim 1 and 2 is characterized in that: described stabilizing agent is epoxidized soybean oil or epoxidized linseed.
6. compounded pesticides according to claim 1 and 2 is characterized in that: described synergist is Butacide, chlorination fat, its ester of carbonic acid alkane or sulfoxide.
7. compounded pesticides according to claim 1 and 2 is characterized in that: described cosolvent is one or more in isoamyl alcohol, phenol, ethyl acetate and the dimethyl sulfoxide (DMSO).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010524617 CN101984828B (en) | 2010-10-29 | 2010-10-29 | Compound pesticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010524617 CN101984828B (en) | 2010-10-29 | 2010-10-29 | Compound pesticide |
Publications (2)
| Publication Number | Publication Date |
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| CN101984828A true CN101984828A (en) | 2011-03-16 |
| CN101984828B CN101984828B (en) | 2013-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201010524617 Expired - Fee Related CN101984828B (en) | 2010-10-29 | 2010-10-29 | Compound pesticide |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535387A (en) * | 2013-10-15 | 2014-01-29 | 黄学松 | Slow-release pesticide |
| CN107950551A (en) * | 2017-12-25 | 2018-04-24 | 云南保山嘉宏农业开发（集团）有限公司 | Pure biology neem-based insecticide preparation process |
| CN107969439A (en) * | 2017-12-25 | 2018-05-01 | 云南保山嘉宏农业开发（集团）有限公司 | Pure biology neem-based insecticide formula |
| CN110269067A (en) * | 2018-03-16 | 2019-09-24 | 成都特普生物科技股份有限公司 | A kind of prevention and treatment agricultural has the combination preparation of pest type |
| CN110447661A (en) * | 2019-08-19 | 2019-11-15 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae in the medicament of the preparation prevention and treatment late blight of potato |
| CN115104632A (en) * | 2022-08-01 | 2022-09-27 | 福建农林大学 | Compound oil suspension emulsion compounded by metarhizium anisopliae and azadirachtin as well as preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1357239A (en) * | 2001-12-27 | 2002-07-10 | 海南英得利生物技术开发有限公司 | Nim extractive and biological pesticide nim prepn |
| CN1802928A (en) * | 2005-12-26 | 2006-07-19 | 重庆大学 | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method |
-
2010
- 2010-10-29 CN CN 201010524617 patent/CN101984828B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1357239A (en) * | 2001-12-27 | 2002-07-10 | 海南英得利生物技术开发有限公司 | Nim extractive and biological pesticide nim prepn |
| CN1802928A (en) * | 2005-12-26 | 2006-07-19 | 重庆大学 | Mixed insecticide of green muscardine fungus and pyrethrum group and its preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 高书晶,等: "杀蝗绿僵菌与植物源农药混用对亚洲小车蝗的杀虫效果", 《农药》, vol. 49, no. 10, 10 October 2010 (2010-10-10), pages 757 - 759 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535387A (en) * | 2013-10-15 | 2014-01-29 | 黄学松 | Slow-release pesticide |
| CN107950551A (en) * | 2017-12-25 | 2018-04-24 | 云南保山嘉宏农业开发（集团）有限公司 | Pure biology neem-based insecticide preparation process |
| CN107969439A (en) * | 2017-12-25 | 2018-05-01 | 云南保山嘉宏农业开发（集团）有限公司 | Pure biology neem-based insecticide formula |
| CN110269067A (en) * | 2018-03-16 | 2019-09-24 | 成都特普生物科技股份有限公司 | A kind of prevention and treatment agricultural has the combination preparation of pest type |
| CN110447661A (en) * | 2019-08-19 | 2019-11-15 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae in the medicament of the preparation prevention and treatment late blight of potato |
| CN110447661B (en) * | 2019-08-19 | 2021-08-03 | 重庆谷百奥生物研究院有限公司 | Application of metarhizium anisopliae in preparation of medicament for preventing and treating potato late blight |
| CN115104632A (en) * | 2022-08-01 | 2022-09-27 | 福建农林大学 | Compound oil suspension emulsion compounded by metarhizium anisopliae and azadirachtin as well as preparation method and application thereof |
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| Publication number | Publication date |
|---|---|
| CN101984828B (en) | 2013-06-12 |
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