CN104488971A - Methylaminoavermectin and lilac pacecilomyces-containing pesticide composition - Google Patents

Methylaminoavermectin and lilac pacecilomyces-containing pesticide composition Download PDF

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Publication number
CN104488971A
CN104488971A CN201410685348.9A CN201410685348A CN104488971A CN 104488971 A CN104488971 A CN 104488971A CN 201410685348 A CN201410685348 A CN 201410685348A CN 104488971 A CN104488971 A CN 104488971A
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China
Prior art keywords
affirm
merck
paecilomyces lilacinus
nematode
pacecilomyces
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CN201410685348.9A
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Chinese (zh)
Inventor
张志伟
刘鹏
冷忠国
吴泽伟
朱刚
王礼文
陈佛祥
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Guangdong Zhongxun Agri Science Corp
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Guangdong Zhongxun Agri Science Corp
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Priority to CN201410685348.9A priority Critical patent/CN104488971A/en
Publication of CN104488971A publication Critical patent/CN104488971A/en
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Abstract

The invention discloses a methylaminoavermectin and lilac pacecilomyces-containing pesticide composition including a commonly used additive, methylaminoavermectin and lilac pacecilomyces, the weight ratio of methylaminoavermectin to lilac pacecilomyces is 1:1 to 1:1000, the sum of the weight percentage of the methylaminoavermectin and the lilac pacecilomyces is 0.1% to 50%. The pesticide composition has obvious synergistic effect, is used for prevention and treatment of nematode of vegetables, peanut, melon, bananas and other crops. The pesticide composition has obvious synergistic effect by compounding of the methylaminoavermectin and the lilac pacecilomyces, reduces the pesticide dosage, saves manpower and material resources, and reduces pesticide pollution to the environment.

Description

A kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus
Technical field
The composition pesticide that the present invention relates to is with Affirm (Merck Co.) and Paecilomyces lilacinus for active ingredient, for preventing and treating crops nematode.
Background technology
Plant nematode mainly comprises: Meloidogyne (Meloidogyne), kidney shape Turbatrix (Rotylenchulus), cyst roundworm (Heterodera (Heterodera)), root spot nematode (short not Turbatrix (Pratykenchus)), causing harm of nematode significantly can reduce the absorption of Plant To Nutrient and moisture, nematode plant of causing harm causes wound, causes phytopathogen to invade.
The chemicals of current control nematode have the high-toxic pesticides such as phonamiphos, carbofuran, terbufos, cadusafos, and this type of agricultural chemicals is because of long-term single use, and nematode is comparatively large to its pesticide resistance, and environmental pollution causes a hidden trouble.
Affirm (Merck Co.), English common name emamectin benzoate, biological source insecticide.This product is efficient, wide spectrum, the longevity of residure are long, and be excellent insecticidal bactericide, its mechanism of action hinders pest movements nerve information transmission and makes health benumb death.There is stomach toxicity and action of contace poison, to crop without interior absorption energy, but effectively infiltrate and use crop epidermal tissue, thus there is the longer longevity of residure.High activity is had to control Lepidoptera, coleoptera, homoptera pest and nematode, and do not produce with other crops and intersect, soil and in easily to degrade noresidue, free from environmental pollution, to beneficial insect and natural enemy, people, animal safety in normal dosage ranges, can with most of combined use of pesticides.
Paecilomyces lilacinus, English common name paecilomyces lilacinus, platymiscium source bactericide, after this medicine buries, spore germination grows a lot of mycelia, mycelia encounters the ovum of nematode, secretion chitinase, thus destroys the periostracum of chorion, mycelia is penetrated chorion, with material in ovum for nutriment amount reproduction, the cell in ovum and body early embryo are damaged, can not larva be hatched.
The mixture of different pesticide species is the common methods of the agriculture resistance disease of control, worm, crop smothering.Screened by formulation of pesticide, filter out rational formula, can effectively improve actual control efficiency, reduce dosage, reducing costs, delay the generation of disease drug resistance, is the important means of disease, worm, the crop smothering comprehensive regulation.The recipe determination of the present inventor to Affirm (Merck Co.) and Paecilomyces lilacinus conducts in-depth research, find that Affirm (Merck Co.) and Paecilomyces lilacinus carry out mixture, certain synergistic effect is had to nematode in certain mixed ratio scope, can effectively prevent and treat crops nematode, after further study, the present invention is completed.
Summary of the invention
The object of the present invention is to provide a kind of efficient, safety, lasting period long, be conducive to the composition pesticide of integrated control crops nematode.
Another object of the present invention is used for preventing and treating crops nematode by this composition pesticide.
Technical solution of the present invention: a kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus, be made up of active ingredient Affirm (Merck Co.) and Paecilomyces lilacinus and suitable insecticides adjuvant, Affirm (Merck Co.) and the Paecilomyces lilacinus weight ratio in composition pesticide is 1:1 ~ 1:1000, and Affirm (Merck Co.) and the percentage by weight of Paecilomyces lilacinus weight summation in composition pesticide are 0.1%-50%.
Composition pesticide of the present invention according to well known to a person skilled in the art method, can be prepared into the application forms of the applicable agricultural uses such as suspending agent, wetting powder, granule.
The auxiliary agent used in the present composition comprises solvent, emulsifier, wetting agent, stabilizing agent, dispersant, thickener, pH adjusting agent, defoamer, antifreezing agent, packing agent etc., and to be of value to active ingredient stable and play the known substance of drug effect in the formulation, be all various compositions that are conventional in pesticidal preparations or permission use, concrete composition and consumption are determined by test according to recipe requirements.
The production technology of the various application forms of composition pesticide of the present invention all belongs to existing known technology, does not repeat them here.
Composition pesticide of the present invention is applicable to the control of crops nematode.
Composition pesticide of the present invention, usually adopt spraying, fill with root, leaching root, seed soaking, dressing, spread fertilizer over the fields, ditch spread, the method for to spread manuer in holes etc. use.
Product described in the invention can the form of finished product preparation provide, namely in composition, each material mixes, the composition of composition also can the form of single dose provide, and for spraying after dilution or carrying out suspending agent, the wetting powder of soil or seed treatment, also can be made into granule and directly use.
The frequency of administration of the present composition and amount of application change with weather conditions and the change of nematode situation of the harm, can by the object using suitable dosage to reach control.
Compared with prior art, the invention has the advantages that: 1, there is obvious synergistic effect, improve control efficiency; 2, damage by disease and insect pesticide resistance is delayed; 3, two kinds of active ingredients are composite, reduce Pesticide use amount, reduce cost and the pollution to environment.
Embodiment
In order to understand essence of the present invention better; below in conjunction with embodiment, content of the present invention is described further; but can not limitation of the present invention be considered as; the following stated is only for explaining the present invention; for not departing from amendment that the present invention's spirit and principle make, replacement or improvement, all belong to the scope of protection of present invention.
A kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus of the present invention can according to well known to a person skilled in the art method, be mixed with suspending agent, wetting powder, granule etc., be applied to agricultural production to use, be described with instantiation below.
Embodiment one: Toxicity Determination is tested
Get the cucumber root producing a large amount of root knot, running water is rinsed well gently, with the milky egg capsule on dissecting needle gently picking old complaint, put into the little culture dish of diameter 6cm, add a small amount of sterile water, in 25 DEG C of insulating boxs, hatch 3-4d, collect 2 instar larvaes, and adding sterile water, to be mixed with the suspension of finite concentration (500/about ml) for subsequent use.
Setting 5 concentration gradient process, take sterile water as blank.Get 96 porocyte culture plates, every hole adds the medicament 150nl of the variable concentrations prepared, add isopyknic nematode suspension again, under 25 DEG C of conditions, moisturizing is cultivated, different time after treatment checks the amount of survival of southern nematode 2 instar and dead quantity respectively, nematode is stiff motionless for dead worm, and nematode is the worm that lives in bending wriggling state.Calculate lethality and corrected mortality.
According to survey data, calculate the corrected mortality of each process.And adopt the abundant method of Sun Yun to calculate the co-toxicity coefficient (CTC value) of mixture with reference to NY/T1154.1-2006.If contrast lethality < 5%, does not correct; Contrast lethality, between 5%-20%, corrects; Contrast lethality > 20%, test need be reformed.Be independent variable x with the logarithm value of drug concentration (mg/L), with the probit value of corrected mortality for dependent variable y, set up virulence regression equation formula respectively, adopt DPS software to calculate the LC of single dose and each proportioning mixture 50, LC 90, 95% confidence limit and mixture co-toxicity coefficient thereof, compare synergy situation.According to the NY/T11547.7-2006 insecticide combination effect criteria for classifying: co-toxicity coefficient (CTC value) >=120 shows as synergistic effect; Co-toxicity coefficient (CTC value)≤120 shows as antagonism; 80 < co-toxicity coefficients (CTC value) < 80 shows as summation action.
Measurement result shows, to nematode CTC value >=120 in the scope of Affirm (Merck Co.) and Paecilomyces lilacinus composite proportioning 1:1 ~ 1:1000, illustrate that Affirm (Merck Co.) and the synergy of Paecilomyces lilacinus mixture to nematode belong to synergistic effect, Affirm (Merck Co.) and Paecilomyces lilacinus is composite has reasonability and feasibility.
Table 1, different ratio Affirm (Merck Co.) and Paecilomyces lilacinus are to the synergy measurement result of Meloidogyne incognita
Embodiment two: 11% Affirm (Merck Co.) Paecilomyces lilacinus suspending agent
Following material is all weight percentage: Affirm (Merck Co.) 0.5%, Paecilomyces lilacinus 10.5%, emulsifier fatty alcohol-polyoxyethylene ether 4%, calcium dodecyl benzene sulfonate 5%, triphenyl vinyl phenol polyoxyethylene poly-oxygen propylene aether 3%, defoamer epoxidized soybean oil 1%, antifreezing agent glycerine 0.5%, surplus is methylated vegetable oil.Above-mentioned formula is added in mixing kettle in proportion successively, after stirring, namely obtains 11% Affirm (Merck Co.) Paecilomyces lilacinus suspending agent.
This embodiment is for preventing and treating cucumber root-knot nematode, and cucumber transplants latter 10 days chemicals treatment, and 11% Affirm (Merck Co.) Paecilomyces lilacinus suspending agent consumption 1,000 grams/acre is watered filling root, and after medicine, 30 days preventive effects are 87.6%.1% Affirm (Merck Co.) microemulsion consumption 1,000 grams/acre is watered filling root, and after medicine, 30 days preventive effects are 72.3%.500000000 spore/gram Paecilomyces lilacinus granule consumption 30,000 grams/acre of ditch spreads alive, after medicine, 30 days preventive effects are 64.7%.
11% Affirm (Merck Co.) Paecilomyces lilacinus oil-suspending agent control crops nematode successful 1% Affirm (Merck Co.) microemulsion and 500,000,000 spore/gram Paecilomyces lilacinus granules alive, illustrate that Affirm (Merck Co.) of the present invention and Paecilomyces lilacinus composition can prevent and treat crops nematode preferably, reduce dosage, reduce costs, delay the drug-fast generation of nematode, reduce environmental pollution.
Embodiment three: 4% Affirm (Merck Co.) Paecilomyces lilacinus wetting powder
Following material is all weight percentage: Affirm (Merck Co.) 2%, Paecilomyces lilacinus 2%, and wetting agent pulls open powder 3%, dispersant naphthalenesulfonate formaldehyde condensation compound 6%, filler talcum powder 15%, and surplus is filler kaolin.Mix entering in blender after above-mentioned formula in proportion coarse crushing, more namely obtain 4% Affirm (Merck Co.) Paecilomyces lilacinus wetting powder after air-flow crushing.
This embodiment is for preventing and treating peanut root-knot nematode, and latter 10 days chemicals treatment transplanted by peanut, and 4% Affirm (Merck Co.) Paecilomyces lilacinus wetting powder consumption 0.4 thousand grams/acre is watered filling root, and after medicine, 30 days preventive effects are 87.2%.1% Affirm (Merck Co.) microemulsion consumption 1,000 grams/acre is watered filling root, and after medicine, 30 days preventive effects are 70.4%.500000000 spore/gram Paecilomyces lilacinus granule consumption 30,000 grams/acre of ditch spreads alive, after medicine, 30 days preventive effects are 66.1%.
4% Affirm (Merck Co.) Paecilomyces lilacinus wetting powder control peanut nematode successful 1% Affirm (Merck Co.) microemulsion and 500,000,000 spore/gram Paecilomyces lilacinus granules alive, illustrate that Affirm (Merck Co.) of the present invention and Paecilomyces lilacinus composition can prevent and treat nematode preferably, reduce dosage, reduce costs, delay the drug-fast generation of nematode, reduce environmental pollution.
Embodiment four: 0.2% Affirm (Merck Co.) Paecilomyces lilacinus granule
Following material is all weight percentage: Affirm (Merck Co.) 0.08%, Paecilomyces lilacinus 0.12%, white carbon 5%, attapulgite 25%, and surplus is kaolin.Above-mentioned formula is carried out in proportion pulverize, drop in agitator stir, the granulation that adds water in granulation disc, drying box drying process, cool, sieve, pack obtained 0.2% Affirm (Merck Co.) Paecilomyces lilacinus granule.
This embodiment is for preventing and treating cucumber root-knot nematode, and cucumber is spread fertilizer over the fields before transplanting, and 0.2% Affirm (Merck Co.) Paecilomyces lilacinus granule consumption 10,000 grams/acre is watered filling root, and after medicine, 30 days preventive effects are 91.7%; 1% Affirm (Merck Co.) microemulsion consumption, 1,000 grams/acre of pesticide-clay mixture spread fertilizer over the fields, and after medicine, 30 days preventive effects are 64.4%; 500000000 spore/gram Paecilomyces lilacinus granule consumptions 30,000 grams/acre alive spread fertilizer over the fields, and after medicine, 30 days preventive effects are 79.2%.
0.2% Affirm (Merck Co.) Paecilomyces lilacinus granule control crops nematode successful is better than 1% Affirm (Merck Co.) microemulsion and 500,000,000 spore/gram Paecilomyces lilacinus granules alive, illustrate that Affirm (Merck Co.) of the present invention and Paecilomyces lilacinus composition can prevent and treat crops nematode preferably, reduce dosage, reduce costs, delay the drug-fast generation of nematode, reduce environmental pollution.

Claims (6)

1. contain a composition pesticide for Affirm (Merck Co.) and Paecilomyces lilacinus, it is characterized in that active ingredient is Affirm (Merck Co.) and Paecilomyces lilacinus, all the other are auxiliary agent.
2. a kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus according to claim 1, is characterized in that: the ratio of weight and number of Affirm (Merck Co.) and Paecilomyces lilacinus is 1:1 ~ 1:1000.
3. a kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus according to claim 1, is characterized in that: Affirm (Merck Co.) and Paecilomyces lilacinus are 0.1% ~ 50% at the percentage by weight of weight summation in composition pesticide.
4. a kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus according to claim 1, is characterized in that: also containing pesticidal preparations auxiliary element in described composition pesticide, to make the application forms being applicable to agriculturally using.
5. a kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus according to claim 1, is characterized in that: its formulation is the one in suspending agent, wetting powder, granule.
6. a kind of composition pesticide containing Affirm (Merck Co.) and Paecilomyces lilacinus according to claim 1, is characterized in that: for preventing and treating crops nematode.
CN201410685348.9A 2014-11-25 2014-11-25 Methylaminoavermectin and lilac pacecilomyces-containing pesticide composition Pending CN104488971A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105901017A (en) * 2016-05-24 2016-08-31 江西天人生态股份有限公司 Agricultural nematicidal composition and application thereof
CN107593774A (en) * 2017-10-17 2018-01-19 惠州市无龄康态健康科技有限公司 A kind of synergy nematicidal composition for being used to prevent and treat groundnut root knot nematode disease
CN110903138A (en) * 2019-11-11 2020-03-24 辽宁丰园肥业有限公司 Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102077846A (en) * 2011-01-21 2011-06-01 佛山市盈辉作物科学有限公司 Nematoid-killing composition containing abamectin and paecilomyces lilacinus
CN103477921A (en) * 2013-10-14 2014-01-01 福建省农业科学院植物保护研究所 Method for comprehensive control of root knot nematode disease of potted ficus microcarpa
WO2014086750A2 (en) * 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102077846A (en) * 2011-01-21 2011-06-01 佛山市盈辉作物科学有限公司 Nematoid-killing composition containing abamectin and paecilomyces lilacinus
WO2014086750A2 (en) * 2012-12-03 2014-06-12 Bayer Cropscience Ag Composition comprising a biological control agent and an insecticide
CN103477921A (en) * 2013-10-14 2014-01-01 福建省农业科学院植物保护研究所 Method for comprehensive control of root knot nematode disease of potted ficus microcarpa

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105901017A (en) * 2016-05-24 2016-08-31 江西天人生态股份有限公司 Agricultural nematicidal composition and application thereof
CN107593774A (en) * 2017-10-17 2018-01-19 惠州市无龄康态健康科技有限公司 A kind of synergy nematicidal composition for being used to prevent and treat groundnut root knot nematode disease
CN110903138A (en) * 2019-11-11 2020-03-24 辽宁丰园肥业有限公司 Microbial selenium-rich pesticide fertilizer for preventing and treating root knot nematode disease, clubroot and underground pests

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