CN114431256A - Compound composition containing pyrethrin and beauveria bassiana and application thereof - Google Patents
Compound composition containing pyrethrin and beauveria bassiana and application thereof Download PDFInfo
- Publication number
- CN114431256A CN114431256A CN202111551377.2A CN202111551377A CN114431256A CN 114431256 A CN114431256 A CN 114431256A CN 202111551377 A CN202111551377 A CN 202111551377A CN 114431256 A CN114431256 A CN 114431256A
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- Prior art keywords
- pyrethrin
- beauveria bassiana
- compound composition
- natural
- treatment
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- VJFUPGQZSXIULQ-XIGJTORUSA-N pyrethrin II Chemical compound CC1(C)[C@H](/C=C(\C)C(=O)OC)[C@H]1C(=O)O[C@@H]1C(C)=C(C\C=C/C=C)C(=O)C1 VJFUPGQZSXIULQ-XIGJTORUSA-N 0.000 title claims abstract description 108
- HYJYGLGUBUDSLJ-UHFFFAOYSA-N pyrethrin Natural products CCC(=O)OC1CC(=C)C2CC3OC3(C)C2C2OC(=O)C(=C)C12 HYJYGLGUBUDSLJ-UHFFFAOYSA-N 0.000 title claims abstract description 107
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- 235000015398 thunder god vine Nutrition 0.000 description 1
- 229960000790 thymol Drugs 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Images
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a compound composition containing pyrethrin and beauveria bassiana and application thereof, belonging to the technical field of pesticides. The effective components of the compound composition comprise pyrethrin and beauveria bassiana, and the mass ratio of the pyrethrin to the beauveria bassiana is (0.1-1.0) to (0.1-4.9). The active ingredients of the pyrethrin and the beauveria bassiana are in a reasonable compounding ratio range, so that the synergistic effect on prevention and treatment of aphids, bemisia tabaci or phyllotreta striolata is achieved.
Description
Technical Field
The invention relates to a compound composition containing pyrethrin and beauveria bassiana and application thereof, belonging to the technical field of biological pesticides.
Background
The pesticide plays an important role in pest control, and with the enhancement of environmental protection and health consciousness, the low-toxicity natural pesticide is gradually replacing the chemical pesticide, and the botanical and microbial pesticide is well received by the market. Pyrethrin is a natural botanical pesticide, is an extract of Pyrethrum cineraiifolium Trev, and contains insecticidal active ingredients such as pyrethrin I and II (pyrethrin I, II), guaifenesin I and II (cinerin I, II), and jasminum I and II (jasmolin I, II). The natural pyrethrin is generally processed into an aqueous emulsion for preventing and controlling agricultural pests, has the characteristics of high efficiency, quickness and broad spectrum of insecticidal property, and is environment-friendly. However, natural pyrethrins are expensive and have insufficient long-lasting effectiveness against pests, limiting their use in the agricultural field.
Beauveria bassiana is an important entomopathogenic fungus, has broad-spectrum insecticidal property and can parasitize more than 1000 kinds of insects. Taxonomically, Beauveria bassiana belongs to Fungi in the kingdom of Fungi, Ascomycota in Ascomycota, Sordariomycetes in Chaetomycetales, Hypocrea in Hypocrea, Cordyceps in Cordycipicaceae, Beauveria in Beauveria. Its conidia are often processed into powder, wettable powder, granule, oil suspension and dispersible oil suspension for preventing and treating agricultural and forestry pests, such as pine moth, corn borer, etc. The beauveria bassiana insecticide has the advantages of being natural, non-toxic, free of drug resistance, easy to produce, low in price and environment-friendly, but the large-scale application of the beauveria bassiana insecticide is restricted by the defects of poor quick-acting performance, unstable drug effect and the like.
If the advantages of the pyrethrin and the beauveria bassiana can be brought into play, the respective defects are made up, and the compounded agent which has the advantages of quick and lasting insecticidal effect and lower price is developed, the compound agent has broad market prospect and generates remarkable economic benefit.
About few researches on the mixing and synergism of pyrethrin, research papers report that the mixing of pyrethrin and plant-derived pesticides (such as matrine and celastrus angulatus) has synergism on aphids or spider mites (the Tibet geese and the like, the pyrethrin and the matrine have the effect of controlling apple red spiders, tobacco terrace fruit trees 2005, (04): 27-28; Liuyuqing and the like, the natural celastrus angulatus and the natural pyrethrin are mixed to match the toxicity and the field control effect of three aphids, the plant protection 2014, 40(02): 175-; the combination of pyrethrin and agricultural antibiotic abamectin or pleocidin has synergistic effect on aphid (Su dynasty, etc., the combined toxicity of abamectin and 4 kinds of biological pesticides on binary aphid, proceedings of wheat crops, 2020, 40(05): 630-.
There are some chinese invention patents (for example, application nos.: CN202110087491.8, CN202110485980.9, CN201910628344.x, CN201910075192.5, CN201910628344.x, CN201810221506.3, CN201811476401.9, CN201710832259.6, cn201510631211.x, CN201310113097.2, etc.) which disclose the preparation technology of insecticidal and bactericidal composition containing pyrethrin, including the technology of mixing pyrethrin with plant-derived materials (such as celastrol, allyl isothiocyanate, azadirachtin, veratrine, cnidium fruit element, berberine, eucalyptus oil, thymol, peppermint, tea tree oil, eugenol, capillary artemisia oil, eucalyptus oil, rosemary essential oil, lavender oligosaccharide, agilawood powder, garlic, tripterygium wilfordii, pimiento, pinellia tuber, etc.) or other natural materials (such as chitooligosaccharide, amino oligosaccharide, gibberellin, sodium sulfonate, abamectin, etc.) and chemical pesticides (such as clothianidin). Other patents (e.g. CN202110714348.7, CN202010832727.1, CN201911148141.7, CN201811251887.6, etc.) disclose biological organic fertilizers containing pyrethrum active ingredients and methods for preparing the same.
However, studies on the mixing of pyrethrins with entomopathogenic fungi have rarely been reported. The Wangcfeng and the like research the biocompatibility of the pyrethrin and the beauveria bassiana, and find that the pyrethrin does not influence the hypha growth of the strain of the beauveria bassiana XJBb3005 and promotes sporulation, but has an inhibiting effect on spore germination (Wangcfeng and the like, the research on the compatibility of 5 botanical pesticides and the beauveria bassiana, reported in tea, 2015, 56(04): 244-. The Chinese invention patent (pyrethroid and biological fungus matching mosquito killer and the manufacturing method thereof, application number: CN201811284644.2) discloses a mosquito killing composition mixed by pyrethroid and pythium biocenoides.
Therefore, through the comprehensive research progress at present, the development of a mixed preparation of natural pyrethrin and beauveria bassiana is blank. The invention aims to develop a mixed pesticide of natural pyrethrin and beauveria bassiana to promote the development of biological pesticide and make a contribution to improving the environmental quality and food safety.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a compound composition of which the active ingredients comprise pyrethrin and beauveria bassiana.
The invention also aims to provide application of the compound composition containing the pyrethrin and the beauveria bassiana in controlling agricultural pests.
In order to achieve the purpose, the invention adopts the technical scheme that: a compound composition containing pyrethrin and beauveria bassiana comprises the effective components of the pyrethrin and the beauveria bassiana. The beauveria bassiana in the compound composition is high-purity beauveria bassiana conidium powder (high spore powder).
As a preferred scheme of the compound composition containing the pyrethrin and the beauveria bassiana, the mass ratio of the combination of the pyrethrin and the beauveria bassiana high spore powder is (0.1-1.0) to (0.1-4.9); namely, 0.1 to 1.0 g of pyrethrin is mixed with 1.77 to 86.73 hundred million/g of beauveria bassiana conidia.
As a preferred scheme of the compound composition containing the pyrethrin and the beauveria bassiana, the mass ratio of the pyrethrin to the beauveria bassiana is (0.2-0.8) to (0.3-1.8); that is, 02-0.8 g of pyrethrin is mixed with 5.31-31.86 hundred million conidia/g of beauveria bassiana conidia.
As a preferable scheme of the compound composition containing the pyrethrin and the beauveria bassiana, the pyrethrin is an extract of natural pyrethrum, and the effective components are a mixture of pyrethrin I, pyrethrin II, guayulin I, guayulin II, jasminuin I and jasminuin II.
As a preferred scheme of the compound composition containing the pyrethrin and the beauveria bassiana, the dosage form of the compound composition is a dispersible oil suspending agent, an oil suspending agent or an ultra-low volume agent.
In addition, the invention also provides application of the compound composition containing the pyrethrin and the beauveria bassiana in preventing and controlling agricultural pests.
As a preferable scheme of the application of the invention, the agricultural pests comprise aphids, bemisia tabaci or phyllotreta striolata.
Compared with the prior art, the invention has the beneficial effects that:
(1) the invention provides a compound composition containing pyrethrin and beauveria bassiana, which is an environment-friendly pesticide composition, and the active ingredients of the pesticide composition, namely the pyrethrin and the beauveria bassiana, have a synergistic effect on prevention and treatment of aphids, bemisia tabaci or phyllotreta striolata within a reasonable compound proportion range;
(2) through the compounding of the active ingredients of the pyrethrin and the beauveria bassiana, the defects that the natural pyrethrin with a single component is expensive in price, the effect on pests is not durable, the quick-acting performance of the beauveria bassiana is poor, the drug effect is unstable and the like are effectively overcome; the compound composition containing the pyrethrin and the beauveria bassiana provided by the invention can realize large-scale industrial production.
Drawings
FIG. 1 is a graph showing the results of treatment of aphid with 1.5% aqueous natural pyrethrin emulsion and 1.5% aqueous dispersible oil suspension of pyrethrin and beauveria bassiana; wherein FIG. 1A is a blank control; FIG. 1B illustrates the treatment of aphids with a 1.5% aqueous emulsion of natural pyrethrin; FIG. 1C shows the treatment of aphid with 1.5% pyrethrin-Beauveria bassiana dispersible oil suspension.
FIG. 2 is a graph showing the results of treatment of Phyllotreta striolata with 1.5% pyrethrin-Beauveria bassiana dispersible oil suspending agent and 0.2% pyrethrin-Beauveria bassiana ultra-low volume oil suspending agent; wherein, the fig. 2A shows the treatment of the phyllotreta striolata with 1.5 percent of pyrethrin-beauveria bassiana dispersible oil suspending agent; FIG. 2B is the treatment of Phyllotreta striolata with 0.2% pyrethrin Beauveria bassiana ultra-low volume oil suspending agent; fig. 2C is a blank pair.
FIG. 3 is a graph showing the results of treating Bemisia tabaci with 1.5% natural pyrethrin aqueous emulsion and 1.5% pyrethrin Beauveria bassiana dispersible oil suspension; FIG. 3A is a blank control; FIG. 3B is a treatment of Bemisia tabaci with a 1.5% natural pyrethrin aqueous emulsion; FIG. 3C shows the treatment of Bemisia tabaci with 1.5% pyrethrin Beauveria bassiana dispersible oil suspension.
Detailed Description
To better illustrate the objects, aspects and advantages of the present invention, the present invention will be further described with reference to the following detailed drawings and examples.
Example 1: preparation and quality detection of beauveria bassiana high spore powder
The preparation method comprises the steps of taking Beauveria bassiana BbPs01 strain (GDMCC NO:61494 in Guangdong province collection center of microorganism strains), fermenting by using rice as a substrate, harvesting conidia, and freeze-drying to obtain high-purity conidia powder (high-spore powder). The spore content of the pure spore powder is measured to be (1.77 +/-0.31) multiplied by 10 by adopting a standard method of the national standard GB/T25864-2010 Beauveria bassiana powder11The germination rate of conidium is (93.75 +/-1.92)%, the drying decrement is (2.53 +/-0.43)%, and the rate of mixed bacteria is 1.05%.
Example 2: biocompatibility of natural pyrethrin to Beauveria bassiana BbPs01 strain
Preparing plant blend oil containing pyrethrin with different concentration gradients of total effective components from natural pyrethrin crude oil (manufactured by Yunnan treasure Biotechnology Limited liability company, total effective component content is 71.78%) for later use, wherein the concentrations are 1000mg/L, 2000mg/L, 5000mg/L, 10000mg/L and 15000mg/L respectively, weighing 30mg pure spore powder in 20mL plant blend oil containing pyrethrin, using the plant blend oil without pyrethrin as a control, standing at normal temperature, sealing with sealing film, and repeating each treatment for 3 times. The spore germination rates were determined after 1d, 15d and 30d of standing, and the results are shown in Table 1.
The results in Table 1 show that the natural pyrethrin crude oil does not significantly reduce the spore germination rate of beauveria bassiana when the dosage is not more than 10000mg/L, and only reduces the spore germination rate by about 3 percent compared with the control even under the dosage treatment of up to 15000 mg/L. Thus, the natural pyrethrin crude oil is safe against beauveria bassiana.
TABLE 1 Effect of crude oil of Natural pyrethrin on spore germination of Beauveria bassiana BbPs01 Strain
Note: the data in the table are mean ± sd, and different letters after the same column indicate significant differences at P < 0.05 level.
Example 3: combined virulence of natural pyrethrin and beauveria bassiana BbPs01 strain
The tested pest is aphis gossypii Glover collected from the experimental farm of southern China university of agriculture, and bred on healthy cucumber for experiment after stable propagation for multiple generations, and the adult with consistent growth period is selected for bioactivity determination while maintaining proper illumination, humidity and temperature. Referring to agricultural industry Standard "NY/T1154.9-2008 indoor bioassay Standard for pesticides test criteria insecticides part 9: spray method, and part 7 of indoor bioassay test guidelines for pesticides, NY/T1154.7-2006: combined action assay for compounding. The method comprises the steps of treating the cucumber leaves by adopting an in-vitro leaf spraying method, shearing healthy cucumber leaves with similar sizes, placing the cucumber leaves into culture dishes, placing 1 leaf in each dish, paving filter paper at the bottom of the dish to preserve moisture, picking 20 aphid adults with the same size on each leaf, treating the aphid adults with the same size by using a Potter spraying tower (British Burgard) according to the dosage of 3mL liquid medicine of each leaf, spraying 0.02% Tween-80 solution on a control leaf, placing the control leaf into a light culture box (Zhujiang LRH-400A-GSI-L3) to culture, wherein the set conditions of light irradiation 14:10(L: D), the temperature of 25 +/-1 ℃ and the relative humidity of 70 +/-10%. Aphid death was observed every 24h and was determined as death with immobility of the extremities of aphids. The number of dead insects was counted and repeated 4 times per treatment. And solving a toxicity equation and a semi-lethal dose according to a logarithmic probability method. The combined effect of the combination was evaluated using a synergistic virulence index (CF) with reference to the method of Mansour et al (1966), where CF ≧ 20 indicates potentiation, CF ≦ 20 indicates antagonism, and 20 ≧ CF ≧ -20 indicates additivity. The CF values were calculated according to the following formula, where the mortality rates were all corrected, MM represents the mortality rate of the mixture, PM represents the mortality rate of pyrethrin treatment, BM represents the mortality rate of Beauveria bassiana treatment, and the results are shown in Table 2, and the formula for CF is as follows:
the results in Table 2 show that when pyrethrin is 0.1-0.9mg/L and beauveria bassiana high spore powder (beauveria bassiana high purity spore powder) is 0.1-4.9mg/L (or 0.18-3.19X 10)8Live spores/mL) are mixed, the combined toxicity of the melon and aphids is different, namely, the synergistic effect is shown in low-dose treatment (the total effective component is 1mg/L) (treatment numbers M0208, M0307, M0406, M0505, M0604 and M0703), and the synergistic effect is also shown in individual 1mg/L dose treatment (M0218); higher doses of treatment (total active ingredient 2-5mg/L) showed additive effects (treatment numbers M0416, M0614, M0812, M0901, M0149, M0337, M0535, M0723, M0921), and only individual combinations showed antagonism 48h after treatment (treatments M0614, M0812 and M0921). LC of the test reagent on the aphid is estimated by virulence regression50At 72h after treatment, the crude oil of natural pyrethrin is 2.48mg/L (95% confidence interval 1.96-2.89), while the LC of Beauveria bassiana500.35(0.19-0.53) mg/L, corresponding to 0.62X 108(0.33×108-0.94×108) spores/mL.
Therefore, the compound composition of the pyrethrin and the conidia of the beauveria bassiana has a synergistic effect on the prevention and treatment of the insect pests of the aphis cucumeria melonis, the mass fraction of the natural pyrethrin is 0.1-1.0%, and the mass fraction of the beauveria bassiana is 0.1-4.9% (or the effective components of the natural pyrethrin are 0.1-1.0 g, and the number of live spores of the beauveria bassiana is 1.77-86.73 hundred million/g). Preferably, pyrethrin 0.2% -0.8%, beauveria bassiana high spore powder 0.3-1.8% (or natural pyrethrin effective component 0.2-0.8 g, beauveria bassiana live spore 5.31-31.86 hundred million/g).
TABLE 2 influence of the mixing ratio of Beauveria bassiana spores and pyrethrin crude oil on the aphid activity
Example 4: preparation of 1.5% pyrethrin-beauveria bassiana dispersible oil suspending agent
Weighing the components according to the dosage shown in the table 3, firstly dissolving the natural pyrethrin crude oil and the synergistic amine into a small amount of oil base, then slowly adding the beauveria bassiana high spore powder, oscillating and mixing uniformly, supplementing the oil base, then adding various thickening agents, mixing uniformly, then adding various other components and supplementing the oil base, stirring for 30min at 8000rpm under a homogenizer, and fully and uniformly mixing. Subpackaging, labeling, and storing at low temperature or in a backlight drying manner for later use.
TABLE 31.5% pyrethrin Beauveria bassiana dispersible oil suspension agent component dosage
Example 5: preparation of 0.15% pyrethrin beauveria bassiana ultra-low volume oil suspending agent
Weighing the components according to the dosage shown in the table 4, firstly dissolving the natural pyrethrin crude oil and the synergistic amine into a small amount of oil base, then slowly adding the beauveria bassiana high spore powder, oscillating and mixing uniformly, supplementing the oil base, then adding various thickening agents, mixing uniformly, then adding various other components and supplementing the oil base, stirring for 30min at 8000rpm under a homogenizer, and fully and uniformly mixing. Subpackaging, labeling, and storing at low temperature or in a backlight drying manner for later use.
TABLE 40.2% pyrethrin Beauveria bassiana ultra-low volume oil suspending agent each component dosage
Example 6: activity of preparation containing compound composition of pyrethrin and beauveria bassiana on aphis cucurbitae
The test agents were diluted to the designed concentration with 0.02% tween 80 solution and then the biological activity of the formulations of example 4 and example 5 on aphid was determined according to the method described above, and the results are shown in table 5, table 6 and figure 1.
Test results show that 1.5% and 0.2% pyrethrin beauveria bassiana preparations have higher activity on melon aphids and are equivalent to the activity of a single-component 1.5% pyrethrin aqueous emulsion (Table 5).
Table 6 toxicity regression analysis shows that LC of 1.5% pyrethrin beauveria bassiana dispersible oil suspending agent and 0.15% pyrethrin beauveria bassiana ultra-low volume oil suspending agent in 48 hours and 72 hours50The value is between 4-16mg/L, which is lower than that of a single 1.5 percent pyrethrin aqueous emulsion; therefore, the activity of 1.5 percent of pyrethrin-beauveria bassiana dispersible oil suspending agent and 0.2 percent of pyrethrin-beauveria bassiana ultra-low volume oil suspending agent on the aphis cucurbitae is higher than that of 1.5 percent of natural pyrethrin aqueous emulsion.
As can be seen from FIG. 1, FIG. 1A is a blank control; FIG. 1B shows the treatment of 1.5% natural pyrethrin aqueous emulsion; FIG. 1C shows a 1.5% pyrethrin Beauveria bassiana dispersible oil suspension treatment; the results show that the color of the aphids of the melon treated by the 1.5% pyrethrum aqueous emulsion is changed from green to brownish red, and the color of the aphids of the melon treated by the 1.5% pyrethrin-beauveria bassiana dispersible oil suspending agent is changed into brownish red, and hyphae grow on the surface of the melon (figure 1).
TABLE 5 lethality of different agents to aphids of melon
Note: the data in the table are mean ± sd, and different letters after the same column indicate significant differences at P < 0.05 level.
TABLE 6 toxicity of different agents on aphids of melon
Example 7: activity of preparation containing compound composition of pyrethrin and beauveria bassiana on phyllotreta striolata
Phyllotreta striolata is bred in an insect breeding cage by using fresh cabbage hearts, proper illumination, humidity and temperature are kept, and adults with consistent growth period are selected for biological activity determination after 3-5 days. The phyllotreta striolata in a healthy state with basically consistent size is narcotized by CO2, then transferred to a centrifuge tube, added with liquid medicine (the preparation of examples 4 and 5 and 1.5% natural pyrethrin aqueous emulsion), covered quickly and turned upside down 50 times, transferred to a disposable plastic bowl paved with filter paper, placed at (26 +/-1) DEG C, and the photoperiod 14:10(L: D), relative humidity (95 +/-5)% in a climatic chamber. And (3) feeding the soaked phyllotreta striolata with fresh radish, replacing every two days, recording the death number of the test insects, transferring the dead insects to a culture dish paved with filter paper, and determining whether the test insects die due to fungal infection according to the characteristics of the insects. The control was treated with 0.02% tween-80 solution and repeated three times for each 10 treated beetles, the results of the tests are shown in table 7, table 8 and figure 2.
The test results show that the activity of the three test formulations (the formulations of examples 4 and 5 and the 1.5% natural pyrethrin aqueous emulsion) on phyllotreta striolata is shown in table 7, and compared with the data in table 6, the activity of the three reagents on phyllotreta striolata is lower than that on aphis cucumerinum.
LC of 1.5% pyrethrin Beauveria bassiana dispersible oil suspension after treating Phyllotreta striolata 6d with three test formulations (example 4, example 5 formulations and 1.5% Natural pyrethrin aqueous emulsion)50LC of pyrethrin beauveria bassiana ultra-low volume oil suspending agent with value of 13.4mg/L and 0.2 percent50The values of 18.4mg/L and 26.7mg/L are lower than those of 1.5% of natural pyrethrin aqueous emulsion, and the activity of 1.5% of pyrethrin beauveria bassiana dispersible oil suspending agent and 0.2% of pyrethrin beauveria bassiana ultra-low volume oil suspending agent on phyllotreta striolata is higher than that of 1.5% of natural pyrethrin aqueous emulsion (Table 8).
As can be seen from FIG. 2, FIG. 2A shows the 1.5% pyrethrin Beauveria bassiana dispersible oil suspension treatment; FIG. 2B shows the ultra-low volume oil suspension agent treatment of 0.2% pyrethrin-Beauveria bassiana; FIG. 2C is a control; hyphae grow on the surface of the flea beetle treated by the preparation, and finally the flea beetle is coated by the hyphae (figure 2).
TABLE 7 Activity of different Agents on Phyllotreta striolata
Note: the data in the table are mean ± standard deviation, with non-identical letters after the same column of data indicating significant differences at P < 0.05 level.
TABLE 8 virulence of different agents against phyllotreta striolata
Example 8: activity of preparation containing compound composition of pyrethrin and beauveria bassiana on bemisia tabaci
Adult insects are captured from a B-type Bemisia tabaci (Bemis tabaci B-biotype) population passaged from laboratory feed by using a pest sucker, released into an insect cage internally provided with potted strong cucumber seedlings without plant diseases and insect pests, and the insect quantity is released according to 50 pairs of leaves. Under the conditions of illumination of 14:10(L: D), temperature of 25 +/-1 ℃ and relative humidity of 70 +/-10%, enabling the bemisia tabaci adults to lay eggs for 1D in the cages, and then driving off all the bemisia tabaci adults. The incubation of the eggs of Bemisia tabaci was observed, and the test was carried out until the eggs reached the second instar nymph stage (about 7 days), with the test results shown in Table 9, Table 10 and FIG. 3.
The cucumber leaves infected by the second-instar bemisia tabaci larvae are cut off by a pair of scissors, the length of leaf stalks is kept to be about 1cm, the leaves are treated by the Potter spraying method, the leaf stalks are coated by cotton containing plant nutrient solution after being taken out, the leaves are placed into culture dishes, 1 leaf is placed in each dish, filter paper is laid at the bottom of each dish to preserve moisture, 0.02% Tween-80 solution is treated by a control leaf, and the dish is placed into a light incubator to be cultured under the conditions that the light is 14:10(L: D), the temperature is 25 +/-1 ℃, and the relative humidity is 70 +/-10%. From 2d, nymph death was examined every 48h under a dissecting mirror, and death was determined as dead bodies, i.e. shriveled and withered. Counting the number of dead insects, repeating the treatment for 3 times, and determining the toxicity of the medicament to the second-instar bemisia tabaci.
The results of the tests show that the three test formulations (example 4, example 5 formulation and 1.5% natural pyrethrin aqueous emulsion) have a bemisia tabaci activity as shown in table 9, and that the three agents have a slightly lower bemisia tabaci activity than the data in table 6.
LC of 1.5% pyrethrin beauveria bassiana dispersible oil suspending agent and 0.2% pyrethrin beauveria bassiana ultra-low volume oil suspending agent50The values (6 days after treatment) are respectively 17.7mg/L and 16.0mg/L, which are lower than 24.8mg/L of 1.5 percent of natural pyrethrin aqueous emulsion; it can be seen that 1.5% pyrethrin-beauveria bassiana dispersible oil suspending agent and 0.2% pyrethrin-beauveria bassiana ultra-low volume oil suspending agent have higher activity to bemisia tabaci than 1.5% natural pyrethrin aqueous emulsion (table 10).
As can be seen from FIG. 3, FIG. 3A is a blank control; FIG. 3B is a 1.5% aqueous emulsion of natural pyrethrin against aphid; fig. 3C shows the treatment of bemisia tabaci with 1.5% pyrethrin beauveria bassiana dispersible oil suspending agent, and the body surface of bemisia tabaci treated with the chrysanthemin beauveria bassiana preparation is finally coated with hypha (fig. 3).
TABLE 9 lethality of different agents against Bemisia tabaci
Note: the data in the table are mean ± sd, and different letters after the same column indicate significant differences at P < 0.05 level.
TABLE 10 toxicity of different agents against Bemisia tabaci
In summary, the experimental results show that the compound composition containing the pyrethrin and the beauveria bassiana has a synergistic effect on prevention and treatment of aphids, bemisia tabaci or phyllotreta striolata in a reasonable compound proportion range.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the protection scope of the present invention, and although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (7)
1. A compound composition containing pyrethrin and beauveria bassiana is characterized in that the effective components of the compound composition comprise the pyrethrin and the beauveria bassiana.
2. The compound composition of claim 1, wherein the mass ratio of the pyrethrin to the beauveria bassiana is (0.1-1.0) to (0.1-4.9).
3. The compound composition of claim 2, wherein the mass ratio of the pyrethrin to the beauveria bassiana is (0.2-0.8) to (0.3-1.8).
4. The combination according to any one of claims 1 to 3, wherein the pyrethrin is an extract of natural pyrethrum, and the effective component is a mixture of pyrethrin I, pyrethrin II, guayulin I, guayulin II, jasminum I and jasminum II.
5. The compounded composition of any one of claims 1-3, wherein the dosage form of the compounded composition is a dispersible oil suspending agent, an oil suspending agent or an ultra-low volume agent.
6. The use of a built composition as claimed in any of claims 1 to 5 for controlling agricultural pests.
7. The use of claim 6, wherein the agricultural pest comprises aphids, bemisia tabaci or phyllotreta striolata.
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