CN114431256B - Compound composition containing pyrethrin and beauveria bassiana and application thereof - Google Patents
Compound composition containing pyrethrin and beauveria bassiana and application thereof Download PDFInfo
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- CN114431256B CN114431256B CN202111551377.2A CN202111551377A CN114431256B CN 114431256 B CN114431256 B CN 114431256B CN 202111551377 A CN202111551377 A CN 202111551377A CN 114431256 B CN114431256 B CN 114431256B
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- pyrethrin
- beauveria bassiana
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- VJFUPGQZSXIULQ-XIGJTORUSA-N pyrethrin II Chemical compound CC1(C)[C@H](/C=C(\C)C(=O)OC)[C@H]1C(=O)O[C@@H]1C(C)=C(C\C=C/C=C)C(=O)C1 VJFUPGQZSXIULQ-XIGJTORUSA-N 0.000 title claims abstract description 122
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- 230000000644 propagated effect Effects 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229920005552 sodium lignosulfonate Polymers 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000010677 tea tree oil Substances 0.000 description 1
- 229940111630 tea tree oil Drugs 0.000 description 1
- 235000015398 thunder god vine Nutrition 0.000 description 1
- 229960000790 thymol Drugs 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a compound composition containing pyrethrin and beauveria bassiana and application thereof, belonging to the technical field of pesticides. The effective components of the compound composition comprise pyrethrin and beauveria bassiana, wherein the mass ratio of the pyrethrin to the beauveria bassiana is (0.1-1.0) to (0.1-4.9). The pyrethrin and beauveria bassiana as active ingredients have a synergistic effect on controlling aphids, bemisia tabaci or phyllotreta striolata within a reasonable compound proportion range.
Description
Technical Field
The invention relates to a compound composition containing pyrethrin and beauveria bassiana and application thereof, belonging to the technical field of biological pesticides.
Background
Pesticides play an important role in pest control, and with the enhancement of environmental protection and health consciousness, low-toxicity natural pesticides are gradually replacing chemical pesticides, and plant sources and microbial pesticides are popular in the market. Pyrethrin is a natural botanical pesticide, is an extract of pyrethrum Pyrethrum cinerariifolium trev. And contains insecticidal active ingredients such as pyrethrin I and II (pyretrin I, II), guaranin I and II (cinerin I, II), and heliotropin I and II (jasmin I, II). Natural pyrethrins are generally processed into aqueous emulsions for controlling agricultural pests, have efficient, fast and broad-spectrum insecticidal properties, and are environmentally friendly. However, natural pyrethrins are expensive and have insufficient long-lasting effectiveness against pests, limiting their use in the agricultural field.
Beauveria bassiana Beauveria bassiana is an important entomopathogenic fungus, has broad-spectrum insecticidal property, and can be parasitic on more than 1000 insects. Taxonomically, beauveria bassiana belongs to the fungus kingdom furgi, ascomycota, sordariomycetes, hypocreales, cordycepitaceae, beauveria, beauveria. The conidium is often processed into powder, wettable powder, granules, oil suspending agent and dispersible oil suspending agent, and is used for preventing and controlling agricultural and forestry pests such as pine moth, corn borer and the like. The beauveria bassiana pesticide has the advantages of nature, no toxicity, no drug resistance, easy production, low price and environmental friendliness, but the defects of poor quick-acting property, unstable drug effect and the like limit the large-scale application of the beauveria bassiana pesticide.
If the advantages of both pyrethrin and beauveria bassiana are exerted, the respective defects are overcome, and the compound agent with quick and lasting insecticidal effect and low price is developed, so that the compound agent has wide market prospect and generates remarkable economic benefit.
There are few studies on the synergy of pyrethrin compounding, and research papers report that the compounding of pyrethrin and botanical pesticides (such as matrine and celastrine) has synergy on aphids or spider mites (Lin Guiyan and the like, the effect test of pyrethrin and matrine on apple red spiders, tobacco stand fruit trees, 2005, (04): 27-28; liu Yuqing and the like, the toxicity and field effect of natural celastrine and natural pyrethrin on three aphids, plant protection, 2014, 40 (02): 175-178; hu Guilei and the like, the combined biological activity and field effect of pyrethrin and matrine compounding on wheat binary aphids, pesticides, 2021, 60 (11): 836-839); the combination of pyrethrin and agricultural antibiotics avermectin or spinosad has synergistic effect on aphids (Su Xu, etc., avermectin and 4 biological pesticides are compounded to the combined virulence of wheat binary aphids, wheat crop school, 2020, 40 (05): 630-637; hu Guilei, etc., the combination of pyrethrin and spinosad is compounded to the combined virulence of wheat binary aphids and field efficacy, henan academy of science (Nature science edition), 2021, 49 (01): 41-45).
Some Chinese patent inventions (such as application numbers: CN202110087491.8, CN202110485980.9, CN201910628344.X, CN201910075192.5, CN201910628344.X, CN201810221506.3, CN201811476401.9, CN201710832259.6, CN201510631211.X, CN201310113097.2 and the like) disclose a preparation technology of insecticidal and bactericidal compositions containing pyrethrin, comprising a technology of mixing pyrethrin with plant source substances (such as celastrin, allyl isothiocyanate, azadirachtin, veratrine, osthole, berberine, eucalyptus oil, thymol, peppermint, tea tree oil, eugenol, capillary artemisia oil, eucalyptus oil, rosemary essential oil, lavender, linalool, garlic, tripterygium wilfordii, capsicum annuum, pinellia ternate and the like) or other natural substances (such as chitosan oligosaccharide, amino oligosaccharins, gibberellins, sodium lignosulfonate, abamectin and the like) and chemical pesticides (such as clothianidin). While other patents (such as CN202110714348.7, CN202010832727.1, CN201911148141.7, CN201811251887.6, etc.) disclose bio-organic fertilizers containing pyrethrum active ingredients and methods of preparing the same.
However, studies on the compounding of pyrethrins with entomopathogenic fungi have been rarely reported. Wang Dingfeng and the like have studied the biocompatibility of pyrethrin with Beauveria bassiana, and found that pyrethrin does not affect the hypha growth of Beauveria bassiana XJBb3005 strain and promotes spore production, but has an inhibiting effect on spore germination (Wang Dingfeng and the like, 5 plant-derived pesticides and Beauveria bassiana compatibility studies, tea theory, 2015, 56 (04): 244-248). Chinese patent (pyrethrin and biological fungus inter-working mosquito killer and its preparing process, application number: CN 201811284644.2) discloses a kind of mosquito killing composition of pyrethrin and Pythium gracile.
Therefore, the research and development of the mixed preparation of the natural pyrethrin and beauveria bassiana are blank after comprehensive current research and development. The invention aims to develop a natural pyrethrin and beauveria bassiana mixed pesticide to promote the development of biological pesticides and make a contribution to improving the environmental quality and the food safety.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a compound composition with active ingredients comprising pyrethrin and beauveria bassiana.
The invention also aims to provide an application of the compound composition containing pyrethrin and beauveria bassiana in controlling agricultural pests.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: a compound composition containing pyrethrin and beauveria bassiana comprises the effective components of pyrethrin and beauveria bassiana. The beauveria bassiana in the compound composition is high-purity beauveria bassiana conidium powder (high-spore powder).
As the preferable scheme of the compound composition containing pyrethrin and beauveria bassiana, the mass ratio of the pyrethrin to the beauveria bassiana high spore powder is (0.1-1.0) to (0.1-4.9); namely, 0.1-1.0 g of pyrethrin is compounded with 1.77-86.73 hundred million/g of beauveria bassiana conidium.
As the preferable scheme of the compound composition containing pyrethrin and beauveria bassiana, the mass ratio of the pyrethrin to the beauveria bassiana is (0.2-0.8) to (0.3-1.8); namely, the pyrethrin is compounded with 5.31-31.86 hundred million/gram of beauveria bassiana conidium by 02-0.8 gram.
As a preferable scheme of the compound composition containing pyrethrin and beauveria bassiana, the pyrethrin is an extract of natural pyrethrin, and the effective components are a mixture of pyrethrin I, pyrethrin II, guaiferin I, guaiferin II, guaiferin I and guaiferin II.
As the preferable scheme of the compound composition containing pyrethrin and beauveria bassiana, the dosage form of the compound composition is dispersible oil suspending agent, oil suspending agent or ultra-low volume agent.
In addition, the invention also provides application of the compound composition containing pyrethrin and beauveria bassiana in controlling agricultural pests.
As a preferred embodiment of the application according to the invention, the agricultural pests include aphids, bemisia tabaci or phyllotreta striolata.
Compared with the prior art, the invention has the beneficial effects that:
(1) The invention provides a compound composition containing pyrethrin and beauveria bassiana, which is an environment-friendly pesticide composition, and the active ingredients of the pyrethrin and beauveria bassiana have a synergistic effect on preventing and controlling aphids, bemisia tabaci or phyllotreta striolata within a reasonable compound proportion range;
(2) The compound of the active ingredients of pyrethrin and beauveria bassiana effectively solves the defects of high price, non-persistent effect on pests, poor quick acting performance, unstable drug effect and the like of the natural pyrethrin with single ingredient; the invention provides a compound composition containing pyrethrin and beauveria bassiana, which can realize large-scale industrial production.
Drawings
FIG. 1 is a graph of the results of treatment of melon aphids with a 1.5% natural pyrethrin aqueous emulsion and a 1.5% pyrethrin beauveria bassiana dispersible oil suspension; wherein FIG. 1A is a blank; FIG. 1B is a treatment of melon aphids with a 1.5% natural pyrethrin aqueous emulsion; FIG. 1C is a treatment of melon aphids with 1.5% pyrethrin beauveria bassiana dispersible oil suspension.
FIG. 2 is a graph of the results of treatment of phyllotreta striolata with 1.5% pyrethrin beauveria bassiana dispersible oil suspension and 0.2% pyrethrin beauveria bassiana ultra-low volume oil suspension; FIG. 2A is a graph of treatment of phyllotreta striolata with 1.5% pyrethrin beauveria bassiana dispersible oil suspension; FIG. 2B is a treatment of phyllotreta striolata with 0.2% pyrethrin beauveria bassiana ultra-low volume oil suspension; fig. 2C is a blank pair.
FIG. 3 is a graph of the results of treatment of bemisia tabaci with a 1.5% natural pyrethrin aqueous emulsion and a 1.5% pyrethrin beauveria bassiana dispersible oil suspension; FIG. 3A is a blank; FIG. 3B is a treatment of Bemisia tabaci with a 1.5% natural pyrethrin aqueous emulsion; FIG. 3C is a treatment of Bemisia tabaci with 1.5% pyrethrin beauveria bassiana dispersible oil suspension.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the following specific drawings and examples.
Example 1: preparation and quality detection of beauveria bassiana high spore powder
Taking beauveria bassiana BbPs01 strain (GDMCC NO:61494 of the collection of microorganism strains of Guangdong province), fermenting with rice as a matrix, harvesting conidium, and freeze-drying to obtain high-purity conidium powder (high-spore powder). The method adopts the standard method of national standard GB/T25864-2010 and beauveria bassiana powder to measure that the spore content of the pure spore powder is (1.77+/-0.31) multiplied by 10 11 The activity (germination rate) of the conidium per gram is (93.75+/-1.92)%, the drying decrement is (2.53+/-0.43)%, and the mixed bacteria rate is 1.05%.
Example 2: biocompatibility of natural pyrethrin on beauveria bassiana BbPs01 strain
The crude oil of natural pyrethrin (produced by Yunnan Bao biotechnology Co., ltd., total active ingredient content is 71.78%) is used for preparing plant blend oil with different concentration gradients of total active ingredient of pyrethrin for later use, the concentrations are respectively 1000mg/L, 2000mg/L, 5000mg/L, 10000mg/L and 15000mg/L, 30mg of pure spore powder is weighed into 20mL of plant blend oil containing pyrethrin, the plant blend oil without pyrethrin is used as a reference, the plant blend oil is placed at normal temperature, and the sealing film is sealed, and each treatment is repeated for 3 times. Spore germination rates were measured after 1d, 15d and 30d, and the results are shown in table 1.
The results in Table 1 show that natural pyrethrin crude oil does not significantly reduce the spore germination rate of beauveria bassiana at doses not greater than 10000mg/L, even at doses as high as 15000mg/L, only about 3% lower than the control. Thus, it is demonstrated that crude natural pyrethrin is safe to beauveria bassiana.
TABLE 1 Effect of Natural pyrethrin crude oil on spore germination of beauveria bassiana BbPs01 Strain
Note that: the data in the table are mean ± standard deviation, and the different letters after the same column of data indicate significant differences at P < 0.05 level.
Example 3: combined virulence of natural pyrethrin and beauveria bassiana BbPs01 strain
The tested insect is Aphis gossypii (Aphisgossypii Glover), collected from agricultural university of south China experiment farm, fed onto healthy cucumber, stably propagated for multiple generations for experiment, kept proper illumination, humidity and temperature, and selected adult with consistent growth period for biological activity measurement. Pesticide part 9 with reference to agricultural industry Standard "NY/T1154.9-2008 pesticide indoor biological assay test guidelines: spray method, "NY/T1154.7-2006 pesticide indoor bioassay test guidelines pesticide section 7: the combined action of the compounding is carried out. In-vitro leaf spraying treatment is adopted, cucumber leaves with similar and healthy sizes are cut and placed into a culture dish, 1 leaf of each dish is paved on the bottom of the dish for moisturizing, 20 aphid adults with consistent sizes are picked on the leaves, 3mL of liquid medicine is used for each leaf, a Potter spray tower (BURKARD) is used for treatment, 0.02% Tween-80 solution is sprayed on control leaves, and the control leaves are placed into an illumination incubator (LRH-400A-GSI-L3) for cultivation, wherein the cultivation setting conditions are illumination 14:10 (L: D) and the temperature 25+/-1 ℃ and the relative humidity 70+/-10%. Aphid death was observed every 24 hours, and the death was determined as the death of the aphid limb. The number of dead insects was counted and repeated 4 times per treatment. The virulence equation and the semi-lethal dose were calculated according to the log-probability method. The combined effect of the cocktail was evaluated by the synergistic toxicity index (CF) with CF.gtoreq.20 indicating potentiation, CF.gtoreq.20 indicating antagonism, 20.gtoreq.CF.gtoreq.20 indicating additive effect, according to the method of Mansource et al (1966). The CF value is calculated according to the following formula, wherein the mortality is a correction value, MM represents the mortality of the mixture, PM represents the mortality of pyrethrin treatment, BM represents the mortality of beauveria bassiana treatment, and the result is shown in Table 2, and the calculation formula of CF is as follows:
as shown in the results of Table 2, when pyrethrin 0.1-0.9mg/L and beauveria bassiana high spore powder (beauveria bassiana high purity conidium powder) 0.1-4.9mg/L (or 0.18-3.19X10) 8 When live spores/mL) are mixed, the combined toxicity of the two types of melon aphids is different, namely, the low-dose treatment (the total effective component is 1 mg/L) shows synergistic effect (treatment numbers M0208, M0307, M0406, M0505, M0604 and M0703), and the individual 1mg/L dose treatment (M0218) also shows synergistic effect; higher doses of treatment (total active ingredient 2-5 mg/L) showed additive effects (treatment numbers M0416, M0614, M0812, M0901, M0149, M0337, M0535, M0723, M0921), only the individual combinations showed antagonism 48h after treatment (treatments M0614, M0812 and M0921). LC of the test agent on melon aphid was estimated by virulence regression 50 At 72h post treatment, the crude natural pyrethrin oil was 2.48mg/L (95% confidence interval 1.96-2.89), while beauveria bassiana LC 50 0.35 (0.19-0.53) mg/L, corresponding to 0.62X10 8 (0.33×10 8 -0.94×10 8 ) spores/mL.
From the results, the compound composition of pyrethrin and beauveria bassiana conidium has a synergistic effect on controlling the insect melon aphid, the mass fraction of natural pyrethrin is 0.1% -1.0%, the mass fraction of beauveria bassiana high spore powder is 0.1-4.9% (or the effective component of natural pyrethrin is 0.1-1.0 g, and beauveria bassiana viable spores are 1.77-86.73 hundred million/g). Preferably, 0.2 to 0.8 percent of pyrethrin, 0.3 to 1.8 percent of beauveria bassiana high spore powder (or 0.2 to 0.8 gram of natural pyrethrin active ingredient, 5.31 to 31.86 hundred million beauveria bassiana viable spores per gram).
TABLE 2 influence of the mixing ratio of Beauveria bassiana spores and pyrethrin crude oil on Aphis melo Activity
Example 4: preparation of 1.5% pyrethrin-beauveria bassiana dispersible oil suspension
Weighing the components according to the dosage of Table 3, dissolving natural pyrethrin crude oil and synergistic amine into a small amount of oil base, slowly adding beauveria bassiana high spore powder, oscillating and mixing uniformly, supplementing the oil base, adding various thickening agents, mixing uniformly, adding various other components and supplementing the oil base, stirring at 8000rpm under a homogenizer for 30min, and fully and uniformly mixing. Packaging, labeling, and storing at low temperature or drying in a backlight for use.
TABLE 3 1.5% pyrethrin beauveria bassiana dispersible oil suspension
Example 5: preparation of 0.15% pyrethrin-beauveria bassiana ultralow-volume oil suspension
Weighing the components according to the dosage of Table 4, dissolving natural pyrethrin crude oil and synergistic amine into a small amount of oil base, slowly adding beauveria bassiana high spore powder, oscillating and mixing uniformly, supplementing the oil base, adding various thickening agents, mixing uniformly, adding various other components and supplementing the oil base, stirring at 8000rpm under a homogenizer for 30min, and fully and uniformly mixing. Packaging, labeling, and storing at low temperature or drying in a backlight for use.
TABLE 4 0.2% pyrethrin beauveria bassiana ultra-low volume oil suspension
Example 6: activity of formulations containing a combination of pyrethrin and beauveria bassiana against Aphis melo
The test agent was diluted to a designed concentration with 0.02% tween 80 solution, and then the biological activity against melon aphids of the formulations of example 4 and example 5 was determined with reference to the previous method, and the test results are shown in table 5, table 6 and fig. 1.
The test results show that the 1.5% and 0.2% pyrethrin beauveria bassiana preparations have higher activity against melon aphids, which is equivalent to the 1.5% pyrethrin aqueous emulsion of a single ingredient (table 5).
TABLE 6 toxicity regression analysis shows that 1.5% and 0.15% of the ultra-low volume oil suspensions were LC at 48 and 72 hours 50 A value between 4 and 16mg/L, less than a single 1.5% pyrethrin aqueous emulsion; it can be seen that 1.5% of pyrethrin beauveria bassiana dispersible oil suspension and 0.2% of pyrethrin beauveria bassiana ultralow-volume oil suspension have activity higher than 1.5% of natural pyrethrin aqueous emulsion for melon aphid.
As can be seen from fig. 1, fig. 1A is a blank; FIG. 1B is a 1.5% natural pyrethrin aqueous emulsion treatment; FIG. 1C is a 1.5% pyrethrin beauveria bassiana dispersible oil suspension treatment; the results show that the color of the melon aphid treated with 1.5% pyrethrum aqueous emulsion is changed from green to brownish red, and the color of the melon aphid treated with 1.5% pyrethrin beauveria bassiana dispersible oil suspension agent is changed to brownish red, and mycelia grow on the body surface (figure 1).
TABLE 5 mortality of different Agents on Aphis melo
Note that: the data in the table are mean ± standard deviation, and the different letters after the same column of data indicate significant differences at P < 0.05 level.
TABLE 6 virulence of different Agents against Aphis melo
Example 7: activity of formulations containing a combination of pyrethrin and beauveria bassiana on phyllotreta striolata
Yellow flea beetle Phyllotreta striolata is fed with fresh cabbage in an insect cage, proper illumination, humidity and temperature are maintained, and adults with the same growth period are selected for biological activity measurement after 3-5 days. The phyllotreta striolata with basically consistent size and healthy state is anesthetized by CO2, then transferred into a centrifuge tube, added with liquid medicine (the preparation of example 4 and example 5 and 1.5% natural pyrethrin aqueous emulsion), quickly covered and inverted up and down for 50 times, transferred into a disposable plastic bowl paved with filter paper, placed at (26+/-1) DEG C, photoperiod 14:10 (L: D) feeding in an artificial climatic chamber having a relative humidity of 95.+ -. 5%. The yellow flea beetles after the soaking treatment are fed with fresh radishes, the fresh radishes are replaced every two days, meanwhile, the death number of the test insects is recorded, the dead insects are transferred into a culture dish paved with filter paper, and whether the test insects are infected by fungi or not is determined to be killed according to the body characteristics of the insects. Treatment with 0.02% tween-80 solution served as a control, 10 head worms per treatment were repeated three times, and the test results are shown in table 7, table 8 and fig. 2.
The results of the experiment show that the three formulations tested (the formulations of example 4, example 5 and 1.5% natural pyrethrin in water emulsion) have lower activity against phyllotreta striolata than the aphid of table 6, as shown in table 7.
After 6d treatment of phyllotreta striolata with three test formulations (formulation of example 4, example 5 and 1.5% natural pyrethrin aqueous emulsion), 1.5% pyrethrin beauveria bassiana dispersible oil suspension LC 50 LC of ultralow-volume oil suspension with value of 13.4mg/L and 0.2% of pyrethrin beauveria bassiana 50 The values of 18.4mg/L, which are lower than 26.7mg/L of 1.5% natural pyrethrin aqueous emulsion, show that the activity of 1.5% pyrethrin beauveria bassiana dispersible oil suspension and 0.2% pyrethrin beauveria bassiana ultralow volume oil suspension on phyllotreta striolata is higher than that of 1.5% natural pyrethrin aqueous emulsion (Table 8).
As can be seen from FIG. 2, FIG. 2A is a 1.5% pyrethrin beauveria bassiana dispersible oil suspension treatment; FIG. 2B is a 0.2% pyrethrin beauveria bassiana ultra-low volume oil suspension treatment; FIG. 2C is a control; the surface of the flea beetle body after the preparation treatment grows hyphae, and finally the flea beetle body is coated with the hyphae (figure 2).
TABLE 7 Activity of different agents against phyllotreta striolata
Note that: the data in the table are mean ± standard deviation, and the different letters after the same column of data indicate significant differences at P < 0.05 level.
TABLE 8 virulence of different agents against phyllotreta striolata
Example 8: activity of formulations containing a combination of pyrethrin and beauveria bassiana on Bemisia tabaci
Adult insects are captured from a B-type bemisia tabaci (Bemisia tabacii B-biotype) population passaged by a fluke device from a laboratory feed, and released into an insect cage of a built-in healthy and insect disease-free cucumber seedling cultivated in a pot, and the insect release amount is 50 pairs per leaf. Under the conditions of illumination of 14:10 (L: D), temperature of 25+/-1 ℃ and relative humidity of 70+/-10%, the bemisia tabaci adults lay eggs in the cages for 1D, and then all the bemisia tabaci adults are repelled. The culture was continued, the hatching condition of the bemisia tabaci eggs was observed, and when the bemisia tabaci eggs developed to the second-age nymph stage (about 7 days), the test was performed, and the test results are shown in table 9, table 10 and fig. 3.
Cutting off cucumber leaves infected by two-age bemisia tabaci larvae with scissors, reserving leaf stalks for about 1cm, treating the leaves by referring to the Potter spraying method, taking out, coating the leaf stalks with cotton containing plant nutrient solution, placing 1 leaf in a culture dish, spreading filter paper on the bottom of the dish for moisturizing, treating 0.02% Tween-80 solution with reference to the leaf, placing in an illumination incubator for culturing, wherein the culture setting conditions are illumination 14:10 (L: D), the temperature is 25+/-1 ℃, and the relative humidity is 70+/-10%. From day 2, the nymphs were examined under a dissecting scope every 48 hours for death, and the body was determined to be flat and yellow. The number of dead insects was counted and 3 replicates per treatment and the virulence of the agent on bemisia tabaci at the second age was determined.
The results of the test show that the three formulations tested (the formulations of example 4, example 5 and 1.5% natural pyrethrin emulsion in water) have slightly lower activity against bemisia tabaci than the data of table 6, as shown in table 9.
LC of 1.5% pyrethrin beauveria bassiana dispersible oil suspension and 0.2% pyrethrin beauveria bassiana ultralow volume oil suspension 50 Values (6 days after treatment) were 17.7mg/L and 16.0mg/L, respectively, below 24.8mg/L for 1.5% natural pyrethrin aqueous emulsion; it can be seen that 1.5% pyrethrin beauveria bassiana dispersible oil suspension and 0.2% pyrethrin beauveria bassiana ultra-low volume oil suspension have an activity on bemisia tabaci higher than 1.5% natural pyrethrin aqueous emulsion (table 10).
As can be seen from fig. 3, fig. 3A is a blank; FIG. 3B is a 1.5% natural pyrethrin emulsion in water versus Aphis melo; FIG. 3C shows the treatment of Beauveria bassiana dispersible oil suspension with 1.5% pyrethrin, beauveria bassiana on Bemisia tabaci, and finally the surface of Bemisia tabaci after treatment with the chrysanthemic beauveria bassiana preparation is coated with hyphae (FIG. 3).
Table 9 mortality of different agents on Bemisia tabaci
Note that: the data in the table are mean ± standard deviation, and the different letters after the same column of data indicate significant differences at P < 0.05 level.
TABLE 10 virulence of different agents against Bemisia tabaci
In summary, experimental results show that the invention provides a compound composition containing pyrethrin and beauveria bassiana, and the active ingredients of the compound composition are in a reasonable compound proportion range, and have a synergistic effect on preventing and controlling aphids, bemisia tabaci or phyllotreta striolata.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.
Claims (1)
1. The application of a compound composition containing pyrethrin and beauveria bassiana in preventing and controlling melon aphids is characterized in that the effective components of the compound composition consist of pyrethrin and beauveria bassiana, wherein the pyrethrin is an extract of natural pyrethrin, and the effective components are a mixture of pyrethrin I, pyrethrin II, guaiferin I, guaiferin II, guaiferin I and guaiferin II;
wherein the mass ratio of the pyrethrin to the beauveria bassiana is 0.5:1, and the spore content of the beauveria bassiana is 1.77 х 10 11 Each gram of the composition contains pyrethrin and beauveria bassiana, and the dosage form of the composition is dispersible oil suspending agent;
or the mass ratio of the pyrethrin to the beauveria bassiana is 0.1:0.1, and the spore content of the beauveria bassiana is 0.124 х 10 11 The dosage form of the compound composition containing pyrethrin and beauveria bassiana is an ultralow-volume oil suspension.
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| CN115152796A (en) * | 2022-08-02 | 2022-10-11 | 福建农林大学 | Beauveria bassiana and pyrethrin composite wettable powder and preparation method thereof |
| CN115316404B (en) * | 2022-09-06 | 2023-09-29 | 浙江宜葆生物科技有限公司 | Dispersible oil suspension containing pyrethrin and beauveria bassiana and application thereof |
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