CN111418604B - Bactericidal composition containing sodium selenite and thiophanate-methyl - Google Patents
Bactericidal composition containing sodium selenite and thiophanate-methyl Download PDFInfo
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- CN111418604B CN111418604B CN202010381613.XA CN202010381613A CN111418604B CN 111418604 B CN111418604 B CN 111418604B CN 202010381613 A CN202010381613 A CN 202010381613A CN 111418604 B CN111418604 B CN 111418604B
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- sodium selenite
- thiophanate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
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- Inorganic Chemistry (AREA)
- Plant Pathology (AREA)
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- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention provides a bactericidal composition containing sodium selenite and thiophanate-methyl, wherein the active ingredients comprise sodium selenite and thiophanate-methyl, and the weight ratio of the thiophanate-methyl to the sodium selenite is 4:1-1: 4; the composition can be used for preventing and treating tobacco diseases.
Description
Technical Field
The invention belongs to the field of agricultural chemistry, and particularly relates to a bactericidal composition containing sodium selenite and thiophanate methyl.
Background
Tobacco root black rot is a soil-borne disease caused by rhizomatomyces Thielaviopsis basicola (berk. and Br.) Ferraris, and occurs in various tobacco regions in China. In recent years, due to the adjustment of tobacco planting area, the tobacco root black rot tends to be increased by the change of the cultivation system and the tobacco variety. The black rot of tobacco root can occur from seedling stage to adult plant stage; mainly occurs on the root system of tobacco plants, which causes black rot symptoms of main roots and lateral roots of tobacco plants in seedling stage and field stage. The seedling stage infection can cause tobacco seedling 'damping-off', but the root part is blackened, so the method is different from damping-off. Pathogenic bacteria invade from the rootstock part, and disease spots surround the stem part for a circle, and invade upwards to the leaves and invade downwards to the lateral roots, so that the whole seedling withers, and the residual seedling without death can also see a small amount of new lateral roots growing at the base part of the young stem above the rotten root, but the development is poor. The disease of tobacco plants in the field period is caused, the leaves of the diseased plants turn yellow from bottom to top, the growth is slow, the plants are dwarfed to different degrees, the yellow leaves of the mild plants are easy to flower early, and the yellow leaves of the severe plants are withered and dead; after the diseased plant is pulled up, most or all of the root system can turn black and rot.
Carbendazim, thiophanate methyl, prochloraz, pyraclostrobin, thiram and the like are common medicaments for preventing and treating the tobacco root black rot in the field, but due to long-term use, the drug resistance of germs is increasingly serious, and the prevention effect is reduced. The chemical pesticide is used for preventing and treating the diseases and insect pests of the tobacco for a long time, on one hand, the prevention and treatment cost is high, the planting cost of tobacco growers is increased, and on the other hand, the risks of environmental pollution, tobacco pesticide residue and the like in the tobacco planting ecological area can be caused. Therefore, the research and development of novel chemical pesticides with high efficiency, low toxicity and low residue will be an important direction for the development of future pesticides.
In addition, current studies indicate that sodium selenite is applied to soil and foliage; the selenium content of the tobacco leaves can be obviously improved; the selenium element can reduce the content of tar and free radicals in cigarette smoke, inhibit tumor occurrence and effectively reduce the incidence rate of lung cancer; meanwhile, the selenium fertilizer has antagonistic action on the poison of heavy metals, thereby reducing the harm to human bodies and improving the safety of tobacco, so that the selenium fertilizer is applied to arouse the wide attention of tobacco researchers. However, there are few reports related to the effect of sodium selenite on tobacco diseases, especially tobacco root black rot. Early-stage research of research teams discovers that sodium selenite has a certain prevention and treatment effect on tobacco root rot (CN108576050A and CN108633913A), a series of screening is carried out on mixed combinations, and the prevention and treatment effect of the compound of sodium selenite and pyraclostrobin is general; the sodium selenite and the prochloraz have an additive effect on the disease; the sodium selenite and the carbendazim are compounded to have obvious synergistic effect. The authors further screened the combination on the basis of earlier studies and selected a more excellent synergistic composition.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides the following technical scheme:
a bactericidal composition containing sodium selenite and thiophanate-methyl is characterized in that active ingredients comprise sodium selenite and thiophanate-methyl, and the weight ratio of the thiophanate-methyl to the sodium selenite is 4:1-1: 4. Preferably, the weight ratio of thiophanate methyl to sodium selenite is 4:1-1: 1; more preferably, the weight ratio of the thiophanate methyl to the sodium selenite is 3:2, 1:1 and 4: 1.
The bactericidal composition also comprises agriculturally acceptable auxiliaries, and the weight percentage of the active ingredients is 1-50%. Preferably 1-35%; more preferably 1 to 25%.
The auxiliary agent comprises one or more of dispersing agent, emulsifying agent, disintegrating agent, stabilizing agent, antifreezing agent, wetting agent, synergist, penetrating agent, preservative, filler and solvent.
The dosage forms which can be prepared by the composition of the invention are wettable powder, water dispersible granules, granules and aqueous emulsion.
The bactericidal composition is used for preventing and treating tobacco diseases. Preferably used for controlling the root rot of tobacco.
Compared with the prior art, the invention has the following beneficial effects:
the bactericidal composition disclosed by the invention is composed of two active ingredients with different action mechanisms, has an obvious synergistic effect after being mixed, improves the prevention and treatment effect, and is also favorable for overcoming and delaying the generation of drug resistance. The composition of the invention is mixed with pesticide to reduce the dosage, thereby reducing the cost and lightening the pollution to the environment, having good safety and meeting the safety requirement of pesticide preparations. The composition of the invention is used for preventing and treating crop diseases, and also provides trace element selenium for tobacco crops, thereby being beneficial to improving the quality of the tobacco crops.
Detailed Description
The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1
1 test Material
1.1 test materials
Tobacco root black rot pathogen [ Thielavirosis basicola (Berk. et br.) Fer ] (separated, identified and preserved by Nicotiana tabacum of Onhua rural institute)
1.2 test Medium
Potato dextrose agar medium (PDA) medium: 200g of potato, 15g of glucose and 15g of agar, and adding water to a constant volume of 1L.
1.3 reagent for test
70% thiophanate-methyl wettable powder (Daguangming agricultural chemical Co., Ltd.)
Sodium selenite (Na2Se03)
2 test method
2.1 Effect of Single dose on hypha growth of Rhizoctonia solani
The inhibition effect of 2 bactericides on the hypha growth of the tobacco root black rot pathogen is measured by adopting a hypha growth rate method in aged spring, the 2 bactericides are respectively prepared into 6 mass concentration gradient mother liquor, sterile water is used as a contrast, 6mL of prepared medicament is respectively sucked and added into 54mL of melted PDA culture medium, the mixture is uniformly shaken and respectively poured into medicament-containing flat plates, a bacterium plate is prepared by a puncher and is respectively placed in the center of the medicament-containing PDA flat plates, 3 treatments are repeated for each treatment, the constant temperature culture is carried out for 7d at 25 ℃, the hypha growth diameter of the strain under different concentration gradient treatments of different medicaments is measured by a cross method, the hypha growth inhibition rate is calculated by a formula, the logarithm of the concentration is taken as an independent variable, the probability value of the relative inhibition rate is taken as a longitudinal coordinate, a standard curve is drawn by utilizing DPS software, and the toxicity regression equation and the effective inhibition medium concentration (EC50) value of the 2 medicaments on the tobacco root black rot pathogen are calculated, the magnitude of virulence of the 2 bactericides was compared according to the EC50 values. Hypha growth inhibition rate (control group diameter-treated group diameter)/(control hypha growth diameter-4)
2.2 formulation screening of Complex combinations
According to the toxicity test results of the 2 bactericides, sodium selenite and thiophanate-methyl are compounded according to different proportions of 1:1, 1:4, 4:1, 3:2, 1: 2 and 5 effective components respectively, and the experimental method is the same as the above. And drawing a standard curve by using DPS software, calculating a regression equation, correlation coefficients and EC50 of the toxicity of each group of compound medicaments to the tobacco root black rot pathogen, calculating the cotoxicity coefficient (CTC) of each group of compound medicaments according to a Sun-Pepper method, and comparing the toxicity effect of the compound bactericide to the tobacco root black rot pathogen. Wherein, when the CTC is more than 120, the toxicity of the compound medicament to the tobacco root rot germ is measured to have synergy, and when the CTC is less than 80, the toxicity is antagonistic. The co-toxicity coefficient (CTC) is calculated as follows: co-toxicity coefficient (CTC) ═ a EC50 × B EC50/M EC50 × (PA × B EC50+ PB × a EC50) where a and B represent two single doses respectively, M represents a mixed dose of the two single doses, and PA and PB represent the ratio of a and B in the mixed dose respectively.
3 results of the test
TABLE 1 Effect of different fungicides on hypha growth of Nicotiana tabacum
The results in Table 1 show that 2 bactericides have certain inhibition effect on hypha growth of the rhizoctonia solani, thiophanate-methyl bacteriostasis effect, EC50 is 2.0331 mu g/mL, sodium selenite inhibition effect is relatively poor, and EC50 is 3.7822 mu g/mL.
TABLE 2 Effect of different combinations on the hypha growth of the black rot of tobacco
The combination of thiophanate methyl and sodium selenite is 3:2, 1:1 and 4:1, which show certain synergistic effect, and the co-toxicity coefficient (CTC) is 120.81, 139.64 and 124.66 respectively.
Example 2: field efficacy test of medicament composition for tobacco root black rot
The test method comprises the following steps: preparing the tobacco root black rot germ after PDA plate culture for 8 days into the proper concentration of 10 with sterile water6-107And (3) inoculating the spore suspension per ml when the tobacco seedlings are transplanted, obliquely cutting the roots of the tobacco with sterilized scissors to form wounds, and taking 20ml of the spore suspension to irrigate the roots and inoculate the roots of the tobacco plants. The application treatment was carried out 10 days after inoculation, and the treatment was repeated 3 times for each 20 strains, and a blank control was set. And 14d, investigating and calculating the control effect.
Test agents:
treatment 1: 5% sodium selenite-thiophanate-methyl wettable powder (sodium selenite: thiophanate-methyl is 1: 1);
comparison 1: 5% sodium selenite carbendazim wettable powder (sodium selenite carbendazim 1: 1).
TABLE 3 field control of tobacco root rot by the combination
The sodium selenite and thiophanate-methyl compounded (1: 1) has excellent prevention and treatment effect on the black rot of tobacco, and the prevention effect is higher than the combination of the sodium selenite and the carbendazim compounded (1: 1).
Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. All percentages in the formulation are by weight. The processing technology of each preparation of the composition is the prior art, and can be changed according to different conditions.
Claims (2)
1. The application of the bactericidal composition containing sodium selenite and thiophanate-methyl for preventing and treating the black rot of tobacco roots is characterized in that active ingredients comprise sodium selenite and thiophanate-methyl, and the weight ratio of the thiophanate-methyl to the sodium selenite is 3:2, 1:1 and 4: 1; the composition also comprises agriculturally acceptable auxiliary agents, and the weight percentage of the active ingredients is 5-50%; the auxiliary agent comprises one or more of dispersing agent, emulsifying agent, disintegrating agent, stabilizing agent, antifreezing agent, wetting agent, synergist, penetrating agent, preservative, filler and solvent.
2. Use according to claim 1, characterized in that: the dosage forms prepared from the composition are wettable powder, water dispersible granules, granules and aqueous emulsion.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010381613.XA CN111418604B (en) | 2020-05-08 | 2020-05-08 | Bactericidal composition containing sodium selenite and thiophanate-methyl |
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| CN202010381613.XA CN111418604B (en) | 2020-05-08 | 2020-05-08 | Bactericidal composition containing sodium selenite and thiophanate-methyl |
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| CN111418604B true CN111418604B (en) | 2022-05-20 |
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| CN113940360B (en) * | 2021-10-15 | 2022-11-01 | 河南农业大学 | Application of sodium selenite in resisting phytophthora nicotianae |
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| CN104230516B (en) * | 2014-07-23 | 2016-06-01 | 山东绿神肥业有限公司 | A kind of facilities vegetable special-purpose irrigation fertilizer and production method thereof |
| CN105130592A (en) * | 2015-08-05 | 2015-12-09 | 大连岭前农业专业合作社 | Cherry tree nutrition injection liquid |
| CN108633913B (en) * | 2018-06-26 | 2020-10-09 | 安徽省农业科学院烟草研究所 | Medicament composition for preventing and treating tobacco root black rot and application thereof |
| CN108576050B (en) * | 2018-06-26 | 2021-04-13 | 安徽省农业科学院烟草研究所 | Application of sodium selenite in preventing and treating tobacco root black rot |
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