CN108576050B - Application of sodium selenite in preventing and treating tobacco root black rot - Google Patents
Application of sodium selenite in preventing and treating tobacco root black rot Download PDFInfo
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- CN108576050B CN108576050B CN201810669702.7A CN201810669702A CN108576050B CN 108576050 B CN108576050 B CN 108576050B CN 201810669702 A CN201810669702 A CN 201810669702A CN 108576050 B CN108576050 B CN 108576050B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
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Abstract
The invention belongs to the field of pesticides, and particularly relates to application of sodium selenite for preventing and treating tobacco root black rot, wherein the sodium selenite and an agriculturally acceptable auxiliary agent are prepared into a proper preparation form.
Description
Technical Field
The invention belongs to the field of pesticides, and particularly relates to application of sodium selenite to prevention and treatment of tobacco root black rot.
Background
A large number of researches show that the selenium element can reduce the content of tar and free radicals in cigarette smoke, inhibit tumor occurrence and effectively reduce the incidence rate of lung cancer; meanwhile, the selenium fertilizer has antagonistic action on the poison of heavy metals, thereby reducing the harm to human bodies and improving the safety of tobacco, so that the selenium fertilizer is applied to arouse the wide attention of tobacco researchers. At present, research on selenium element on tobacco mainly focuses on the influence of selenium on the field growth of tobacco, such as the distribution of selenium on various parts or organs of tobacco, the relation between the selenium content in soil and the selenium content in tobacco leaves, the influence of selenium fertilizer spraying on the growth, development and quality of tobacco, and the like. The research shows that: the selenium content of the tobacco leaves can be obviously improved by applying the sodium selenite to the soil Puff and the leaf surfaces, and the utilization rate of the leaf surface application is dozens of times of that of the soil Puff; the sodium selenite seed soaking treatment with different concentrations has influence on the germination rate, the root system and the photosynthetic pigment content.
A large number of researches show that the silicon can relieve abiotic stress (heavy metal, salt stress and the like) and biotic stress (plant diseases and insect pests) of crops and improve the photosynthesis rate of rice, soybean, wheat and the like under adverse conditions such as drought and the like. The results of the research on the influence of silicon on the resistance of tobacco ring spot and mosaic disease by adopting a water culture test by Zellner and the like show that the addition of potassium silicate can prolong the incubation period of virus and obviously reduce the morbidity and the disease index. Tests of plum blosson and the like show that the application of the silicon fertilizer can obviously improve the yield and quality of tobacco and the resistance to anthracnose and climate spot disease. Studies on Juanjuan et al show that Si has a certain influence on photosynthesis of tobacco.
The black rot of tobacco roots caused by rhizobium rhizogenes basicola (Thielaviopsis basicola) is an important root fungal disease in tobacco production, and in recent years, the tobacco planting area in China has a tendency of increasing harm, and the current prevention and treatment measures mainly depend on chemical agents and bring a series of problems to cigarette sanitation. The invention aims to search for a novel medicament for preventing and treating the tobacco root black rot, and no report about whether sodium selenite or silicon dioxide can be used for preventing and treating the tobacco root black rot exists in the prior art.
Disclosure of Invention
In addition, the sodium selenite is used, so that the purposes of preventing and treating diseases and supplementing selenium elements capable of promoting tobacco growth are achieved, and the labor intensity is reduced.
The invention provides the following technical scheme:
the sodium selenite is used for preventing and treating the black rot of tobacco roots. Preferably, the sodium selenite and the agriculturally acceptable auxiliary agent are prepared into a proper preparation form, and the percentage content of the active ingredients can be 0.1-10%.
The agriculturally acceptable auxiliary materials comprise an emulsifier, a dispersant, a wetting agent, a binder, a disintegrating agent, a thickening agent, a stabilizer, an antifreezing agent, a defoaming agent filler and the like.
The active ingredient can be combined with agriculturally acceptable auxiliary materials to prepare an adaptive dosage form, and preferably wettable powder, a suspending agent, a microemulsion, an emulsion in water or water dispersible granules and the like.
The sodium selenite is used for preventing and treating the tobacco root black rot, and the preferred sodium selenite can be combined with one or more bactericide components.
The invention has the following beneficial technical effects:
the invention provides a new application of sodium selenite, namely an application for preventing and treating tobacco root black rot. In addition, the sodium selenite is used, so that the purposes of preventing and treating diseases and supplementing selenium element capable of promoting the growth of tobacco are achieved, and the labor intensity is reduced.
Detailed Description
The following detailed description of specific embodiments of the present invention is provided in conjunction with examples, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
Example 1 hypha inhibition of sodium selenite and silica against Rhizoctonia solani
The test method comprises the following steps: activating tobacco root black rot bacteria by a medium-containing method, inoculating to PDA, and culturing for 10d until hypha overgrows the surface of the culture medium for use. Accurately weighing the medicament, respectively preparing the sodium selenite and the silicon dioxide into the concentrations of 1ug/mL, 10ug/mL, 100ug/mL, 1000ug/mL and 10000ug/mL, taking sterile water as a negative control, and sterilizing under an ultraviolet lamp for later use. The culture medium is prepared according to the proportion of the medicine to the culture medium of 1:10, 1mL of liquid medicine is taken out by a pipette and is put into a culture dish, 9mL of melted potato agar culture medium is taken and mixed evenly, so that the final concentration of the sodium selenite and the silicon dioxide in the culture medium respectively reaches 0.1ug/mL, 1ug/mL, 10ug/mL, 100ug/mL and 1000ug/mL, and then the plate is poured and placed for solidification for later use. Adopting a growth rate method, beating the cultured tobacco root black rot into lawn by a puncher with the diameter of 5mm, connecting 1 plate of bacteria dish into the center of each drug-containing plate, and repeating each treatment by 3 plates. Inoculating thallus Porphyrae, placing into a 25 deg.C incubator, culturing for 5d, testing by growth rate method, measuring colony diameter by cross method, calculating inhibition rate of the agent on pathogenic bacteria hyphae, processing with DPS data to draw toxicity regression equation, and calculating EC50And a correlation coefficient r value.
TABLE 1 Effect of sodium selenite on hypha growth of Nicotiana tabacum (5d)
TABLE 2 influence of silicon dioxide on the hypha growth of Rhizoctonia solani (5d)
As can be seen from tables 1 and 2, sodium selenite can inhibit the growth of the hypha of the Rhizoctonia solani, while the silica solution has no effect of inhibiting the growth of the hypha of pathogenic bacteria. When the concentration of the sodium selenite is 0.1ug/mL, the inhibition rate of the sodium selenite on the hypha of the black rot pathogen of the tobacco roots is 27.66 percent; when the concentration of the sodium selenite is 1000ug/mL, the inhibition rate of the sodium selenite on the hypha of the black rot fungus of the tobacco roots is 100 percent; namely, the sodium selenite has good inhibition effect on the growth of the black rot germ filaments of the tobacco roots.
Example 2 field control of sodium selenite against black rot of tobacco roots
The test method comprises the following steps: preparing the tobacco root black rot germ after PDA plate culture for 8 days into the proper concentration of 10 with sterile water6-107And (3) inoculating the spore suspension per ml when the tobacco seedlings are transplanted, obliquely cutting the roots of the tobacco with sterilized scissors to form wounds, and taking 20ml of the spore suspension to irrigate the roots and inoculate the roots of the tobacco plants. The application treatment was carried out 10 days after inoculation, and the treatment was repeated 3 times for each 20 strains, and a blank control was set. And 14d, investigating and calculating the control effect.
Test agents: treatment 1: 1% sodium selenite wettable powder; and (3) treatment 2: 1% silicon dioxide wettable powder.
TABLE 3 field control of sodium selenite against black rot of tobacco roots
| Medicament | Dilution factor | Control effect% |
| Process 1 | 500 times of | 85.34 |
| Treatment 2 | 500 times of | 1.36 |
| CK | — | — |
As can be seen from Table 3, the 1% sodium selenite wettable powder has a good prevention and treatment effect on the tobacco root black rot, and the prevention effect is up to 85.34%. The 1% silicon dioxide wettable powder cannot be used for preventing and controlling the tobacco root black rot.
The above description is only a preferred embodiment of the present invention, and it is obvious to those skilled in the art that modifications may be made without departing from the scope of the present invention, and such modifications are also within the scope of the present invention.
Claims (4)
1. Use of sodium selenite for the control of tobacco root black rot, characterized in that the sodium selenite is prepared in a suitable formulation with agriculturally acceptable adjuvants, wherein the minimum concentration of sodium selenite is 10 μ g/ml.
2. Use according to claim 1, wherein the agriculturally acceptable adjuvants comprise one or more of emulsifiers, dispersants, wetting agents, binders, disintegrants, thickeners, stabilizers, antifreeze agents, defoamers, fillers.
3. The use according to claim 1, wherein the formulation is a wettable powder or a water dispersible granule.
4. Use according to any one of claims 1 to 3, wherein sodium selenite is used in combination with one or more fungicide ingredients.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810669702.7A CN108576050B (en) | 2018-06-26 | 2018-06-26 | Application of sodium selenite in preventing and treating tobacco root black rot |
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| CN201810669702.7A CN108576050B (en) | 2018-06-26 | 2018-06-26 | Application of sodium selenite in preventing and treating tobacco root black rot |
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| CN108576050A CN108576050A (en) | 2018-09-28 |
| CN108576050B true CN108576050B (en) | 2021-04-13 |
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| CN201810669702.7A Active CN108576050B (en) | 2018-06-26 | 2018-06-26 | Application of sodium selenite in preventing and treating tobacco root black rot |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418604B (en) * | 2020-05-08 | 2022-05-20 | 安徽省农业科学院烟草研究所 | Bactericidal composition containing sodium selenite and thiophanate-methyl |
| CN113940360B (en) * | 2021-10-15 | 2022-11-01 | 河南农业大学 | Application of sodium selenite in resisting phytophthora nicotianae |
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2018
- 2018-06-26 CN CN201810669702.7A patent/CN108576050B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| "硒对果蔬采后病害的控制及同步生产富硒果实的研究";吴之琳;《中国优秀硕士学位论文全文数据库 农业科技辑》;20160415;摘要，第13-14页 * |
| "采后亚硒酸钠处理对杏果黑斑病的控制及贮藏品质的影响";高春丽 等;《食品科学》;20160108;第37卷(第14期);摘要，第3小节 * |
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