CN1463604A - Pest control agent comprising multiple micro-organism - Google Patents
Pest control agent comprising multiple micro-organism Download PDFInfo
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Abstract
The present invention relates to pest and disease preventing and controlling agent containing several kinds of microbes and its production process. The pest and disease preventing and controlling agent consists of several kinds of biocontrol microbes and beneficial microbes, nutritive liquid for the microbes to grow, plant source medicine and diatomite as adsorbent. It has high and lasting effect, less resistance, less environmental pollution, simple production process and stable quality. It is used in preventing and controlling pests and diseases in crop and city plant and in preventing and controlling roach, mosquito, fly and other pests.
Description
Technical field
The present invention relates to a kind of disease and pest control agent that contains multiple microorganism, and the production method that also relates to this control agent.
Technical background
At present, the medicament great majority that are used for prevention and elimination of disease and pests are chemical pesticide.These synthetic agricultural chemicals have played important effect aspect the preventing and treating of damage by disease and insect, still, its harmfulness also is conspicuous.Moreover access times are many, and the expense that always drops into is many, and drug effect reduces year by year, and the pesticide resistance of damage by disease and insect but strengthens year by year, has lost the due effect of prevention and elimination of disease and pests.In addition, in manufacturing, accumulating and use, continuously contaminated environment is destroyed the ecological balance, the physical and mental health of harm humans.By contrast, the disease and pest control agent that contains multiple microorganism has special efficacy, long-acting, access times are few, and the expense that always drops into is few, are difficult for developing immunity to drugs, the effect of prevention and elimination of disease and pests is comparatively remarkable, and the minimizing environmental pollution, help the ecological balance and human physical and mental health, and production technology is simple, easy to operate, characteristics such as steady quality.
In known technology, contain the disease and pest control agent of microorganism, selected microorganism is single or less.The scope of prevention and elimination of disease and pests is narrower, the quality less stable, and the effect of prevention and elimination of disease and pests is unsatisfactory.Simultaneously, also exist access times many, the expense that always drops into is many, and production technology is numerous and diverse, weak points such as operation inconvenience.
The object of the present invention is to provide a kind of technological process simple, easy to operate, and contain efficient, the broad spectrum activity disease and pest control agent of multiple microorganism.Another object of the present invention is to provide a kind of production method of described control agent.
Summary of the invention
The present invention's multiple microorganism fungus kind has reasonably arranged in pairs or groups, and has given full play to " biological control effect ", " mushroom common prosperity effect ", " hormonal stimulation effect ", and with the advantages such as " synergistic effects " of beneficial microbe and other agricultural chemicals.So, when using the disease and pest control agent that contains multiple microorganism, not only prevention and elimination of disease and pests effectively also can make plant growing healthy and strong, increases the resistivity of plant to damage by disease and insect.The disease and pest control agent that contains multiple microorganism has special efficacy, long-acting, access times are few, total input expense is few, be difficult for developing immunity to drugs, the effect of prevention and elimination of disease and pests is remarkable, and reduces environmental pollution, help the ecological balance and human physical and mental health, and production technology is simple, and is easy to operate, characteristics such as steady quality.
The following describes disease and pest control agent that contains multiple microorganism of the present invention and preparation method thereof.
A kind of disease and pest control agent that contains multiple microorganism is characterized in that it comprises following component:
1, microbial inoculum, it is made up of described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum and described beneficial microbe bacterium liquid, and described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum is at least a bacterium liquid that is selected from bacillus thuringiensis bacterium liquid, pseudomonas fluorescens bacterium liquid, streptomycete bacterium liquid, white muscardine fungi bacterium liquid, green muscardine fungus bacterium liquid and/or the paecilomycerol bacterium liquid; Described beneficial microbe bacterium liquid is selected from least a bacterium liquid in azotobacteria bacterial liquid, phosphobacteria bacterium liquid and/or the potassium bacterium bacterium liquid;
The bacterial classification of wherein said biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum is respectively bacillus thuringiensis (Bacillusthuringiensis subsp AS 1.785,1.995,1.897,1.961,1.1754, ACCC 10016,10024,10025,10028,10033,10061), pseudomonas fluorescens (Pseudmonasfluorescens AS 1.33,1.55,1.867, and ACCC 10040); Light day blue streptomycete (strptomyces coerulescens AS 4.4653 of streptomyces, 4.680,4.929, ACCC 40010), light gray streptomycete (Streptomyces griseolus var AS 4.456,4.507, ACCC 40075), thorn spore streptomyces hygroscopicus (Streptomyces hygrospinosus Yan et Tao ACCC 40015,40016,40017,40033,40051,40068,40071), streptomyces hygroscopicus (Streptomyces hygroospinosus varAS 4.168,4.911,4.1026,4.1004, ACCC40034,40051,40064), celebrate rich streptomycete (Streptomyces qingfengmyceticus Saalmsipp AS 4.1036, ACCC 40078), streptomyces rimosus (Streptomyces rimosus Sobin et al AS 4.748, ACCC 40079); Beauveria beauveria bassiana (Beauveria bassiana Vuillemin AS 3.2055,3.4270,3.4428, ACCC30006,30107,30113), white muscardine fungi (Beauveria sp AS 3.470,3.3474,3.3475,3.3576), powdery white muscardine fungi (Beauveria velatl Samson AS 3.4429); Green muscardine fungus genus blastomyces green muscardine fungus (Metarhizium album Petch ACCC 30198), Metarhizium anisopliae (Metarhiziumanisopliae ACCC 30101,30102,30104,30131,30194), brown green muscardine fungus (Metarhizium brunneum Petch ACCC 30200), post spore green muscardine fungus (Metarhiziumeylindrosporae Chen et Guo ACCC 30114), emerald green green muscardine fungus (Metarhizium iadiniChen ACCC 30124), big spore green muscardine fungus (Metarhizium majarosporae ACCC 30187,30195), flat husky green muscardine fungus (Metarhizium pingshaense Chen et Guo ACCC 30105); Paecilomyces varioti Pseudomonas powder paecilomycerol (Paecilomyces farinosus ACCC 30190, CFCC 5079), paecilomycerol (Paecilomyces sp AS 3.2762,3.3728, ACCC 30147) etc. bacterial strain (China Committee for Culture Collection of Microorganisms, Chinese agriculture bacterial classification catalogue, version in 1991, Chinese agriculture science and technology publishing house, 2-65).These bacterial classifications can be by common micro-organisms DSMZ of China Committee for Culture Collection of Microorganisms, and agriculture microorganism fungus kind preservation center or other units concerned obtain.
The bacterial classification of wherein said beneficial microbe bacterium liquid is respectively azotobacter blown-ball Azotobacter (Azotobacter chroococcum ACCC 10001,10002,10006, AS 1.178,1.212,1.222), Wei Nielande azotobacter (Azotobacter Vinelandii ACCC 10087,10088, AS1.207,1.824,1.1005,1.1649), rhizobium of bean (Rhizobium japonicum ACCC15005,15018,15067, AS 1.174,1.1826,1.1828), rice Flavobacterium (Flovobacteriumoryzae spnov ACCC 10051,10052); (Bacillusmegaterium ACCC 10008,10010,10011 for the bacillus bacillus megaterium, AS 1.127,1.515,1.941), Bacillus cercus (Bacillus cereus AS 1.182,1.1626), colloid bacillus cereus bacterial strain (China Committee for Culture Collection of Microorganisms such as (Bacillus ACCC10012, AS 1.231), Chinese agriculture bacterial classification catalogue, version in 1991, Chinese agriculture science and technology publishing house, 1-15).These bacterial classifications can be by common micro-organisms DSMZ of China Committee for Culture Collection of Microorganisms, and agriculture microorganism fungus kind preservation center or other units concerned obtain.
It is as follows that described microbial inoculum is formed (in weight portion):
Described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum:
Bacillus thuringiensis bacterium liquid 1-30 part;
Pseudomonas fluorescens bacterium liquid 0.1-5 part;
Streptomycete bacterium liquid 1-40 part;
White muscardine fungi bacterium liquid 1-20 part;
Green muscardine fungus bacterium liquid 1-30 part;
Paecilomycerol bacterium liquid 1-5 part.
Described beneficial microbe bacterium liquid:
Azotobacteria bacterial liquid 1-15 part;
Phosphobacteria bacterium liquid 1-10 part;
Potassium bacterium bacterium liquid 1-10 part.
It is 30-50 more than hundred million/milliliter or 10,000,000,000/gram of spore that described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum contains effective bacterium number, and beneficial microbe bacterium liquid contains effective bacterium and counts 30-50 more than hundred million/milliliter.
2, nutrient solution, it is at least a nutrients that is selected from bacterial cultures, trace compound or the plant growth regulator;
Described bacterial cultures is to be selected from glucose, sucrose, peptone, meat extract, glycerine, agar, analysis for soybean powder, mealy potato, starch, CaCO
3, MnSO
4.4H
2O, KH
2PO
4, NaCl, yeast extract, ammonium molybdate, ironic citrate, K
2HPO
4, MgSO
4.7H
2O, FeSO
4.7H
2At least a culture in O or the fused calcium magnesium phosphate.
Described trace compound is at least a compound that is selected from borax, zinc sulphate, ferrous sulfate, copper sulphate, manganese sulphate, ammonium molybdate, cobalt oxide, titanium oxide, selenium oxide or the potassium iodide.
Described plant growth regulator is to be selected from heteroauxin, 2,4-D, at least a plant growth regulator in volume increase element, methyl, sevin, gibberellin, zeatin, kinetin, di-phenylurea, triacontanol, brassin lactones, chlormequat, sodium humate, alar-85 or the paclobutrazol.
The composition of described bacterial cultures, trace compound and plant growth regulator (in weight portion) is as follows:
Bacterial cultures 25-70 part;
Trace compound 5-35 part;
Plant growth regulator 1-15 part.
3, adsorbent, described adsorbent are at least a adsorbents that is selected from kaolin, potter's clay, clay, diatomite, talcum powder, white carbon or the pyrophyllite;
4, other agricultural chemicals, it is to be selected from least a agricultural chemicals in botanical pesticide, animal sources agricultural chemicals, mineral sources agricultural chemicals or the synthetic pesticide.
Described botanical pesticide is to be selected from rotenone, nicotine, pyrethrin, anabasine, jervine, fennel artemisin, sodium alginate, gossylure, juvenile hormone, nimbin, limonoid, caryophyllus oil, citronella oil, β-asarone, sesamin, piperonyl butoxide, heteroauxin, ethene, the basic element of cell division, gibberellin, abscisic acid, brassin lactones, zeatin, 2, at least a agricultural chemicals in 4-D or the methyl.
Described animal sources agricultural chemicals be selected from nereistoxin, Manda draws at least a agricultural chemicals in toxin, cantharidin, moulting hormone, brain hormone, juvenile hormone or the sex pheromone.
Described mineral sources agricultural chemicals is at least a agricultural chemicals that is selected from zinc phosphide, aluminum phosphate or the Kocide SD.
Described synthetic pesticide is to be selected from carbofuran, Aldicarb, Aphox, Bassa, unden, decis, flucythrinate, brofluthrinate, ether chrysanthemum ester, permethrin, diflubenzuron, the young urea that goes out, Teflubenzuron, Buprofezin, dimehypo, desinsection list, cartap, polythiane, food refusal hydrazine, triaguron, arsenic worm quinoline, four mite piperazines, to reach at least a agricultural chemicals in mite ketone, azacyclotin or propargite, ethoprop, Aldicarb or the dazomet.
Containing biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum, beneficial microbe bacterium liquid, nutrient solution, adsorbent and other agricultural chemicals described in the disease and pest control agent of multiple microorganism, to form (in weight portion) as follows:
Described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum 20-35 part;
Described beneficial microbe bacterium liquid 5-15 part;
Described nutrient solution 5-20 part;
Described adsorbent 60-75 part;
Described agricultural chemicals 1-25 part.
The disease and pest control agent that contains multiple microorganism contains multiple internationally recognized special microorganism, and they are only effective to the target disease and insect, is a kind of efficient, broad spectrum activity disease and pest control agent.Have special efficacy, long-acting, access times are few, and the expense that always drops into is few, are difficult for developing immunity to drugs, the effect of prevention and elimination of disease and pests is comparatively remarkable, and reduces environmental pollution, helps the ecological balance and human physical and mental health, and production technology is simple, and is easy to operate, characteristics such as steady quality.The disease and pest control agent that contains multiple microorganism can be widely used in the extermination of disease and insect pest of crop genetics and urban afforestation, and is used to prevent and treat insects such as open-air and indoor cockroach, mosquitos and flies.
The preparation method who contains the disease and pest control agent of multiple microorganism of the present invention may further comprise the steps:
One, preparation bacterium liquid
Preparation biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum is comprising bacillus thuringiensis, pseudomonas fluorescens, streptomycete (for example light day blue streptomycete, the light gray streptomycete, thorn spore streptomyces hygroscopicus, streptomyces hygroscopicus, celebrate rich streptomycete, streptomyces rimosus), white muscardine fungi (beauveria bassiana for example, white muscardine fungi, the powdery white muscardine fungi), green muscardine fungus (for example white green muscardine fungus, Metarhizium anisopliae, the brown green muscardine fungus, post spore green muscardine fungus, emerald green green muscardine fungus, big spore green muscardine fungus, flat husky green muscardine fungus), paecilomycerol (powder paecilomycerol for example, paecilomycerol) preparation of bacterium liquid or spore mixture such as;
Preparation beneficial microbe bacterium liquid is comprising the preparation of azotobacter (for example blown-ball Azotobacter, Wei Nielande azotobacter, rhizobium of bean, rice Flavobacterium), phosphobacteria (for example bacillus megaterium, Bacillus cercus), potassium bacterium bacterium liquid such as (for example colloid bacillus cereus).
Above-mentioned bacterium liquid mixing is obtained preparing the needed bacterium liquid of disease and pest control agent that contains multiple microorganism of the present invention.Its composition (in weight portion) is as follows:
Described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum:
Bacillus thuringiensis bacterium liquid 1-30 part;
Pseudomonas fluorescens bacterium liquid 0.1-5 part;
Streptomycete bacterium liquid 1-40 part;
White muscardine fungi bacterium liquid 1-20 part;
Green muscardine fungus bacterium liquid 1-30 part;
Paecilomycerol bacterium liquid 1-5 part.
Described beneficial microbe bacterium liquid:
Azotobacteria bacterial liquid 1-15 part;
Phosphobacteria bacterium liquid 1-10 part;
Potassium bacterium bacterium liquid 1-10 part.
Two, preparation nutrient solution, described nutrient solution is obtained by bacterial cultures, trace compound and plant growth regulator mixing, and its composition (in weight portion) is as follows:
Bacterial cultures 25-70 part;
Trace compound 5-35 part;
Plant growth regulator 1-15 part.
Three, preparation adsorbent, for example adsorbent powders such as kaolin, potter's clay, clay, diatomite, talcum powder, white carbon and pyrophyllite are broken to granularity less than 0.18 millimeter, behind 120 ℃, 30 minutes autoclaving, obtain needed adsorbent.
Four, to contain effective bacterium number be that 30-50 more than hundred million/milliliter or spore more than 10,000,000,000/gram, described beneficial microbe bacterium liquid contain effective bacterium and counts 30-50 more than hundred million/milliliter, described nutrient solution, described adsorbent and described other agricultural chemicals through fully mixing to described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum, obtain the described disease and pest control agent that contains multiple microorganism, its composition (in weight portion) is as follows:
Above-mentioned biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum 20-35 part;
Above-mentioned beneficial microbe bacterium liquid 5-15 part;
Above-mentioned nutrient solution 5-20 part;
Above-mentioned adsorbent 60-75 part;
Above-mentioned agricultural chemicals 1-25 part.
The description of the following mask body manufacture method that contains the disease and pest control agent of multiple microorganism of the present invention:
One, preparation bacterium liquid
1, slant medium (seed culture medium), the production preparation of medium and bacterium liquid:
A. the preparation method of slant medium: after all raw material weighing with the seed culture based formulas, put into container in the lump, boiled 10-30 minute, adjust pH value 7.0--7.2.To be mixed evenly after, divide to install in the test tube, be filled to 1/3 of test tube.The test tube of 19 * 190mm, charge weight are 15 milliliters.Behind 120 ℃, 30 minutes autoclaving, while hot test tube is tiltedly put the bevel medium.
B. produce preparation method: will produce with after all raw material weighing of culture medium prescription, and put into container in the lump, boil 10-30 minute, and adjust pH value 7.0--7.2 with medium.To be mixed evenly after, divide to install in the triangular flask (500 milliliters triangular flask charge weight is 300 milliliters) or fermentation tank (the fermentation tank charge weight of 1000 liters of capacity is 700 kilograms) through 120 ℃, 30 minutes autoclavings.
C. the preparation method of bacterium liquid: choose the production bacterial classification, generally can choose a strain or multi-strain bacteria strain as required and form a group and produce bacterial strain, it is inoculated into respectively in the corresponding slant medium,, be secondary seed through 25-30 ℃ of shaken cultivation 3-7 days.After secondary seed culture mixed in equal amounts, grain weight by 1-3% is inoculated in the fermentation tank that has agitating device that corresponding production medium is housed, through 25-30 ℃, 100-200 rev/min stir culture 3-7 days make effective bacterium count content and reach hundred million of 30-50 or above/milliliter bacterium liquid or 10,000,000,000/gram of spore.It is stand-by that bacterium liquid prepares the back.
2, the prescription of various bacterium culture mediums and preparation method:
1) culture medium prescription of biological and ecological methods to prevent plant disease, pests, and erosion microorganism fungus kind:
(1) bacillus thuringiensis:
The slant culture based formulas:
Peptone 5 grams, meat extract 3 grams, NaCl5 gram, agar 15 grams, MnSO
4.4H
2O
31000 milliliters of milligram, distilled water, pH 7.0.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
5 kilograms of peptones, NaCl3 kilogram, 10 kilograms in agar, MnSO
4.4H
2100 liters in O0.3 kilogram, water, pH 7.0.
It can adopt aforementioned preparation method B preparation.
Bacillus thuringiensis bacterium liquid adopts aforementioned preparation method C preparation.Generally can choose 1-5 strain bacterial strain as required and form one group of production bacterial strain.
(2) pseudomonas fluorescens:
The slant culture based formulas:
Peptone 20 grams, K
2PO
41.5 gram, MgSO
4.7H
21000 milliliters of O 1.5 grams, glycerine 10 grams, agar 15 grams, distilled water, pH 7.2.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
5 kilograms of peptones, K
2PO
41 kilogram, 4 kilograms in agar, MnSO
4.4H
23 kilograms of O, 100 liters in water, pH 7.0.
It can adopt aforementioned preparation method B preparation.
Pseudomonas fluorescens bacterium liquid adopts aforementioned preparation method C preparation.Generally can choose 1-3 strain bacterial strain as required and form one group of production bacterial strain.
(3) streptomycete, the blue streptomycete in for example light sky, light gray streptomycete, thorn spore streptomyces hygroscopicus, streptomyces hygroscopicus:
The slant culture based formulas:
K
2HPO
45 grams, starch 20 grams, KH
2PO
40.5 gram, MgSO
4.7H
2O 0.5 gram, NaCl 0.5 gram, FeSO
4.7H
21000 milliliters of O 0.01 grams, agar 20 grams, distilled water, pH 7.2-7.4.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
K
2HPO
40.5 kilogram, 2 kilograms of starch, K
2HPO
40.5 kilogram, MgSO
4.7H
20.5 kilogram of 0.5 kilogram of O, NaCl, FeSO
4.7H
20.01 kilogram of O, 2 kilograms in agar, 100 liters in water, pH 7.2-7.4.
It can adopt aforementioned preparation method B preparation.
Streptomycete, the blue streptomycete in for example light sky, light gray streptomycete, thorn spore streptomyces hygroscopicus, streptomyces hygroscopicus bacterium liquid adopt aforementioned preparation method C preparation, generally can choose 1-10 strain bacterial strain as required and form one group of production bacterial strain.
Streptomycete, for example celebrate rich streptomycete, streptomyces rimosus:
The slant culture based formulas:
Peeled potatoes 200 grams add 1000 milliliters in water, boil filter residue, supply 1000 milliliters.Add glucose 20 grams, agar 15 grams again, pH 7.0.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
10 kilograms of peeled potatoes add 30 liters in water, boil filter residue, supply double centner.Add 5 kilograms of glucose, 5 kilograms in agar again, pH 7.0.
It can adopt aforementioned preparation method B preparation.
Streptomycete is for example celebrated rich streptomycete, and streptomyces rimosus bacterium liquid adopts aforementioned preparation method C preparation.Generally can choose 1-6 strain bacterial strain as required and form one group of production bacterial strain.
(4) white muscardine fungi, for example beauveria bassiana, white muscardine fungi, powdery white muscardine fungi:
The slant culture based formulas:
Peeled potatoes 200 grams add 1000 milliliters in water, boil filter residue, supply 1000 milliliters.Add glucose 20 grams, agar 15 grams again, pH 7.0.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
10 kilograms of peeled potatoes add 30 liters in water, boil filter residue, supply double centner.Add 5 kilograms of glucose, 5 kilograms in agar again, pH 7.0.
It can adopt aforementioned preparation method B preparation.
White muscardine fungi, for example beauveria bassiana, white muscardine fungi, powdery white muscardine fungi bacterium liquid adopt aforementioned preparation method C preparation, generally can choose 1-8 strain bacterial strain as required and form one group of production bacterial strain.
(5) green muscardine fungus, for example white green muscardine fungus, Metarhizium anisopliae, brown green muscardine fungus, post spore green muscardine fungus, emerald green green muscardine fungus, big spore green muscardine fungus, flat husky green muscardine fungus:
The slant culture based formulas:
Peeled potatoes 200 grams add 1000 milliliters in water, boil filter residue, supply 1000 milliliters.Add glucose 20 grams, agar 15 grams again, pH 7.0.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
10 kilograms of peeled potatoes add 30 liters in water, boil filter residue, supply double centner.Add 5 kilograms of glucose, 5 kilograms in agar again, pH 7.0.
It can adopt aforementioned preparation method B preparation.
Green muscardine fungus, for example white green muscardine fungus, Metarhizium anisopliae, brown green muscardine fungus, post spore green muscardine fungus, emerald green green muscardine fungus, big spore green muscardine fungus, flat husky green muscardine fungus bacterium liquid adopt aforementioned preparation method C preparation.Generally can choose 1-18 strain bacterial strain as required and form one group of production bacterial strain.
(6) paecilomycerol, for example powder paecilomycerol, paecilomycerol:
The slant culture based formulas:
Peeled potatoes 200 grams add 1000 milliliters in water, boil filter residue, supply 1000 milliliters.Add glucose 20 grams, agar 15 grams again, pH 7.0.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
10 kilograms of peeled potatoes add 30 liters in water, boil filter residue, supply double centner.Add 5 kilograms of glucose, 5 kilograms in agar again, pH 7.0.
It can adopt aforementioned preparation method B preparation.
Paecilomycerol, for example powder paecilomycerol, paecilomycerol bacterium liquid adopt aforementioned preparation method C preparation.Generally can choose 1-5 strain bacterial strain as required and form one group of production bacterial strain.
2) beneficial microbe culture of strains based formulas:
(1) azotobacter, for example blown-ball Azotobacter, Wei Nielande azotobacter, rhizobium of bean, rice Flavobacterium:
The slant culture based formulas:
Glucose 5 grams, glycerine 3 grams, yeast extract 10 grams, KH
2PO
40.5 gram, MgSO
4.7H
2O 0.2 gram, NaCl 0.1 gram, CaCO
33 grams, 1 milliliter of ammonium molybdate, 1 milliliter of ironic citrate, 1 milliliter of CoO, MnSO
4.4H
21000 milliliters of 1 milliliter of O, agar 15-20 gram, distilled water, pH 6.5-7.5.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
2 kilograms of sucrose, 2 kilograms of glucose, 2 kilograms of glycerine, 3 kilograms of analysis for soybean powder, 1 kilogram in fused calcium magnesium phosphate, 1 kilogram of ash, 100 liters in water, pH 6.5-7.5.
It can adopt aforementioned preparation method B preparation.
Azotobacter, for example blown-ball Azotobacter, Wei Nielande azotobacter, rhizobium of bean, rice Flavobacterium bacterium liquid adopt aforementioned preparation method C preparation.Generally can choose 1-8 strain bacterial strain as required and form one group of production bacterial strain.
(2) phosphobacteria, for example bacillus megaterium, Bacillus cercus:
The slant culture based formulas:
Peptone 5 grams, meat extract 3 grams, NaCl 3 grams, MnSO
4.4H
21000 milliliters of O 0.5 grams, glycerine 10 grams, mealy potato 5 grams, agar 10-15 gram, distilled water, pH 6.5-7.5.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
1 kilogram of 1 kilogram of KCl, NaCl, MgSO
4.7H
21 kilogram of O, 0.5 kilogram of ironic citrate, CaCO
31 kilogram, 1 kilogram of mealy potato, 2 kilograms in agar, 100 liters in water, pH 6.5-7.5.
It can adopt aforementioned preparation method B preparation.
Phosphobacteria, for example bacillus megaterium, Bacillus cercus bacterium liquid adopt aforementioned preparation method C preparation.Generally can choose 1-5 strain bacterial strain as required and form one group of production bacterial strain.
(3) potassium bacterium, for example colloid bacillus cereus:
The slant culture based formulas:
Glucose 10 grams, CaCO
31 gram, MnSO
4.4H
2O 0.2 gram, peptone 10 grams, KH
2PO
40.5 1000 milliliters of grams, NaCl 3 gram, agar 10-15 gram, distilled water, pH 6.5-7.5.
It can adopt aforementioned preparation method A preparation.
Produce and use culture medium prescription:
1 kilogram of sucrose, 1 kilogram of glucose, MgSO
4.7H
20.5 kilogram of O, KH
2PO
40.5 kilogram, 2 kilograms of analysis for soybean powder, 1 kilogram in agar, 100 liters in water, pH 6.5-7.5.
It can adopt aforementioned preparation method B preparation.
The potassium bacterium, for example colloid bacillus cereus bacterium liquid adopts aforementioned preparation method C preparation.Generally can choose 1-2 strain bacterial strain as required and form one group of production bacterial strain.
Two, prescription of nutrient solution and preparation method thereof:
Nutrient solution among the present invention helps the needs of mentioned microorganism breeding and plant growing, mainly is made up of bacteriotrophy thing, trace compound and/or plant growth regulator.
Bacterial cultures mainly contains glucose, sucrose, peptone, meat extract, glycerine, agar, analysis for soybean powder, mealy potato, starch, CaCO
3, MnSO
4.4H
2O, KH
2PO
4, NaCl, yeast extract, ammonium molybdate, ironic citrate, K
2HPO
4, MgSO
4.7H
2O, FeSO
4.7H
2At least a culture in O or the fused calcium magnesium phosphate.
Trace compound mainly contains at least a compound in borax, zinc sulphate, ferrous sulfate, copper sulphate, manganese sulphate, ammonium molybdate, cobalt oxide, titanium oxide, selenium oxide or the potassium iodide.
Plant growth regulator mainly contains heteroauxin, 2, at least a plant growth regulator in 4-D, volume increase element, methyl, sevin, gibberellin, zeatin, kinetin, di-phenylurea, triacontanol, brassin lactones, chlormequat, sodium humate, alar-85 or the paclobutrazol.
According to the difference of the needs and the geographical environment of microorganism fungus kind, plant variety and growth, selectable composition (in weight portion) is as follows:
Bacterial cultures 25-70 part;
Trace compound 5-35 part;
Plant growth regulator 1-15 part.
The concrete preparation method of nutrient solution is as follows: take by weighing earlier above-mentioned needed raw material respectively, put into container in the lump, add the water of 100 parts (in weight portions) more separately, boiled 30 minutes, solution is adjusted to pH value 7.0--7.2, promptly make nutrient solution after stirring.
Three, preparation of adsorbent: with raw material, for example silt in kaolin, potter's clay, clay, diatomite, talcum powder, white carbon and the pyrophyllite etc. and foreign material are removed totally, and oven dry is crushed to granularity less than 0.18 millimeter.Autoclaving through 120 ℃, 30 minutes, the cooling back is standby.
Four, preparation
The nutrient solution of above-mentioned preparation is added in the described adsorbent (for example kaolin, potter's clay, clay, diatomite, talcum powder, white carbon and/or pyrophyllite etc.), start agitator, stirred 5-15 minute with 50-250 rev/min speed, until stirring, again through 120 ℃, 30 minutes autoclaving; Then, again with biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum (contain effective bacterium number and be 30-50 more than hundred million/milliliter or 10,000,000,000/gram of spore) and beneficial microbe bacterium liquid (contain effective bacterium number and be 30-50 more than hundred million/milliliter) and one or more other agricultural chemicals (for example botanical pesticide, animal sources agricultural chemicals, mineral sources agricultural chemicals and/or synthetic pesticide), through fully mixing, can be made into the disease and pest control agent that contains multiple microorganism of the present invention.
Perhaps with described nutrient solution through 120 ℃, behind 30 minutes the autoclaving, when adding biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum (contain effective bacterium number and be 30-50 more than hundred million/milliliter or 10,000,000,000/gram of spore) and beneficial microbe bacterium liquid (contain effective bacterium number and be 30-50 more than hundred million/milliliter), described nutrient solution is added in the above-mentioned adsorbent, add one or more other agricultural chemicals (botanical pesticide for example again, the animal sources agricultural chemicals, mineral sources agricultural chemicals and/or synthetic pesticide), speed with 50-250 rev/min, stirred 5-15 minute, until stirring, can be made into the disease and pest control agent that contains multiple microorganism of the present invention.
The composition (in weight portion) of described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum, beneficial microbe bacterium liquid, nutrient solution, adsorbent and other agricultural chemicals is as follows:
Biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum 20-35 part;
Beneficial microbe bacterium liquid 5-15 part;
Nutrient solution 5-20 part;
Adsorbent 60-75 part;
Agricultural chemicals 1-25 part.
The disease and pest control agent that contains multiple microorganism of the present invention has following good effect:
Can be widely used in the extermination of disease and insect pest of crop genetics and urban afforestation, and be used to prevent and treat insects such as open-air and indoor cockroach, mosquitos and flies.
Use in the process of crop genetics and urban afforestation, the incidence of damage by disease and insect is obviously reduced, the lethality of various pests is more than 95%.Simultaneously, also can promote growth and development of plant, strengthen resistivity damage by disease and insect.
Use in the process of insects such as the open-air and indoor cockroach of control, mosquitos and flies, the incidence of insects such as cockroach, mosquitos and flies is obviously reduced, lethality is more than 95%.
Can increase the quantity of the microorganism sociales in the natural environment.
Help the environmental protection and the ecological balance, help human physical and mental health.
Now with disease and pest control agent and other agricultural chemicals the results are shown in the following table of containing multiple microorganism of the present invention in the contrast of aspects such as properties of product, implementation result, economy:
| Access times | Folding money (unit/mu) | Cockroach (unit/100m 2) | The scope of application | Using method | Product quality | |
| Other agricultural chemicals | Repeatedly repeat | ????50-250 | ????20-35 | Limitation | Complicated | Reduce |
| The control agent | 1-2 time | ????25-50 | ????5-20 | Extensively | Easy | Improve |
| Soil regime | Environmental protection | Production technology | Investment | Profit | Energy resource consumption | Communications and transportation | |
| Other agricultural chemicals | Destroy | Pollute | Numerous and diverse | Huge | Seldom | Huge | Heavy |
| The control agent | Improvement | Purify | Advanced | Special little | Huge | Minimum | Reduce |
Can clearly be seen that from last table the disease and pest control agent that contains multiple microorganism of the present invention has very significantly good effect than other agricultural chemicals that uses in the prior art.
Embodiment
Below by the detailed specific description the present invention of embodiment, rather than limitation of the present invention.
Embodiment 1:
1, the selection of bacterial classification:
1) the biological and ecological methods to prevent plant disease, pests, and erosion microorganism is bacillus thuringiensis AS 1.785, ACCC 10016,10061, and white muscardine fungi AS 3.470, emerald green green muscardine fungus ACCC 30124, paecilomycerol AS 3.2762 bacterial strains are as the production bacterial strain.
2) beneficial microbe is blown-ball Azotobacter ACCC 10001, AS 1.178, Wei Nielande azotobacter AS 1.1005, rhizobium of bean ACCC 15005, AS 1.174, rice Flavobacterium ACCC 10051, bacillus megaterium ACCC 10008, AS 1.127, Bacillus cercus AS 1.182, colloid bacillus cereus ACCC 10012 bacterial strains are as the production bacterial strain.
2, adopt preceding method to prepare slant medium, produce medium and prepare needed bacterium liquid.Get 30 kilograms of bacillus thuringiensis bacterium liquid, 20 kilograms of white muscardine fungi bacterium liquid, 30 kilograms of green muscardine fungus bacterium liquid, 5 kilograms of paecilomycerol bacterium liquid, 5 kilograms of azotobacteria bacterial liquids, 5 kilograms of phosphobacteria bacterium liquid, 5 kilograms of potassium bacterium bacterium liquid, amount to double centner.
3, adopt preceding method to prepare adsorbent, 360 kilograms of preparation diatomite adsorbants.
4, adopt preceding method to prepare nutrient solution.Get 20 kilograms of nutrient solutions, it is as follows that its nutrients is formed (in weight portion): sucrose 5, glucose 5, analysis for soybean powder 5, peptone 1, meat extract 1, starch 2, NaCl0.2, MnSO
4.4H
2O 0.054, K
2HPO
45, KH
2PO
40.6, MgSO
4.7H
2O 0.6, FeSO
4.7H
2O 0.055, agar 5, borax 0.5, zinc sulphate 0.5, heteroauxin 0.002, volume increase element 0.054, gibberellin 0.002, zeatin 0.002.
5, other agricultural chemicals is got 1 kilogram of pyrethrin, 1 kilogram of nereistoxin, 1 kilogram of nimbin, 0.5 kilogram of juvenile hormone, 0.5 kilogram of β-asarone, 0.5 kilogram of sex pheromone, 1 kilogram of food refusal hydrazine, 0.5 kilogram of zeatin, amounts to 6 kilograms.
6, adsorbent is put into agitator for 360 kilograms, add described bacterium liquid double centner, 20 kilograms of nutrient solutions, 6 kilograms in agricultural chemicals again.Then, start agitator, stirred 5-15 minute,, just make 486 kilograms of disease and pest control agents that contain multiple microorganism until stirring with 50-250 rev/min speed.
Adopt the disease and pest control agent that this kind contains multiple microorganism to test, the incidence of damage by disease and insect is obviously reduced, and the lethality of insects such as aphid, plant hopper, leafhopper, thrips, aleyrodid, coccid, liriomyza bryoniae, weevil, stinkbug silk worm, cabbage caterpillar is more than 95%.Also effective for soil insect.The above-mentioned disease and insect that chemicals is developed immunity to drugs, this product has special efficacy.Simultaneously, also can promote growth and development of plant, strengthen resistivity damage by disease and insect.
Embodiment 2:
1, the selection of bacterial classification:
1) the biological and ecological methods to prevent plant disease, pests, and erosion microorganism is pseudomonas fluorescens AS 1.33, ACCC 10040, light day blue streptomycete AS4.4653, ACCC 40010, light gray streptomycete AS 4.456, ACCC 40075, thorn spore streptomyces hygroscopicus ACCC 40015, streptomyces hygroscopicus AS 4.168, celebrate rich streptomycete AS 4.1036, streptomyces rimosus AS4.748, bacillus thuringiensis AS 1.1754, ACCC 10028,10033, white muscardine fungi AS 3.470, powdery white muscardine fungi AS 3.4429, post spore green muscardine fungus ACCC 30114, emerald green green muscardine fungus ACCC 30124, big spore green muscardine fungus ACCC 30187, flat husky green muscardine fungus ACCC 30105, paecilomycerol AS 3.3728 bacterial strains are as the production bacterial strain.
2) beneficial microbe is blown-ball Azotobacter ACCC 10006, AS 1.222, and Wei Nielande azotobacter ACCC 10088, rice Flavobacterium ACCC 10052, bacillus megaterium AS 1.516, colloid bacillus cereus AS 1.231 bacterial strains are as the production bacterial strain.
2, adopt preceding method to prepare slant medium, produce medium and prepare needed bacterium liquid.Get 30 kilograms of streptomycete bacterium liquid, 5 kilograms of pseudomonas fluorescens bacterium liquid, 10 kilograms of white muscardine fungi bacterium liquid, 15 kilograms of bacillus thuringiensis bacterium liquid, 10 kilograms of green muscardine fungus bacterium liquid, 1 kilogram of paecilomycerol bacterium liquid, 3 kilograms of azotobacteria bacterial liquids, 3 kilograms of phosphobacteria bacterium liquid, 3 kilograms of potassium bacterium bacterium liquid, amount to 80 kilograms.
3, adopt preceding method to prepare adsorbent, 220 kilograms in preparation kaolin adsorbent.
4, adopt preceding method to prepare nutrient solution.Get 10 kilograms of nutrient solutions, it is as follows that its nutrients is formed (in weight portion): peptone 2, K
2PO
40.6, MgSO
4.7H
2O 0.9, glycerine 2, glycerine 3, yeast extract 2, KH
2PO
40.8, NaCl 0.1, CaCO
31, ammonium molybdate 0.056, ironic citrate 0.1, MnSO
4.4H
2O 0.54, mealy potato 15, starch 5, agar 6, cobalt oxide 0.5, titanium oxide 0.4, fused calcium magnesium phosphate 10, kinetin 0.001, di-phenylurea 0.001, triacontanol 0.001, brassin lactones 0.0011.
5, other agricultural chemicals, get 1 kilogram of sesamin, 1 kilogram of rotenone, 1 kilogram of propargite, 1 kilogram of limonoid, 0.5 kilogram of moulting hormone, 0.5 kilogram of gossylure, 0.5 kilogram of sex pheromone, 1 kilogram of food refusal hydrazine, 1 kilogram of dazomet, 0.5 kilogram of the basic element of cell division, amount to 8 kilograms.
6, adsorbent is put into agitator for 220 kilograms, add 80 kilograms of described bacterium liquid, 10 kilograms of nutrient solutions, 8 kilograms in agricultural chemicals again.Then, start agitator, stirred 5-15 minute with 50-250 rev/min speed, until stirring.Just make 318 kilograms of disease and pest control agents that contain multiple microorganism.
Adopt the disease and pest control agent that this kind contains multiple microorganism to test, the incidence of damage by disease and insect is obviously reduced, prevent and treat root rot, take-all, rust, rice blast, banded sclerotial blight, multiple diseases such as cotton seedling blight and tobacco leaf rust have remarkable result; The lethality of insects such as aphid, plant hopper, leafhopper, thrips, aleyrodid, coccid, liriomyza bryoniae is more than 95%.The above-mentioned disease and insect that chemicals is developed immunity to drugs, this product has special efficacy.Simultaneously, also can promote growth and development of plant, strengthen resistivity damage by disease and insect.
Embodiment 3:
1, the selection of bacterial classification:
The biological and ecological methods to prevent plant disease, pests, and erosion microorganism is white green muscardine fungus ACCC 30198, Metarhizium anisopliae ACCC 30102,30104,30131, brown green muscardine fungus ACCC 30200, post spore green muscardine fungus ACCC 30114, emerald green green muscardine fungus ACCC 30124, big spore green muscardine fungus ACCC 30195, flat husky green muscardine fungus ACCC 30105, powder paecilomycerol ACCC 30190, CFCC 5079, paecilomycerol AS 3.3728, ACCC 30147, bacillus thuringiensis AS 1.897, ACCC 10025,10028, light gray streptomycete ACCC 40075, beauveria bassiana AS 3.4270, ACCC 30107,30113, white muscardine fungi AS 3.3475, powdery white muscardine fungi AS3.4429 bacterial strain is as the production bacterial strain.
2, adopt preceding method to prepare slant medium, produce medium and prepare needed bacterium liquid.Get 30 kilograms of green muscardine fungus bacterium liquid, 15 kilograms of bacillus thuringiensis bacterium liquid, 5 kilograms of white muscardine fungi bacterium liquid, 5 kilograms of paecilomycerol bacterium liquid, 5 kilograms of streptomycete bacterium liquid, amount to 60 kilograms.
3, adopt preceding method to prepare adsorbent, get 190 kilograms of clay absorbents.
4, adopt preceding method to prepare nutrient solution.Get 5 kilograms of nutrient solutions, it is as follows that its nutrients is formed (in weight portion): glucose 5, CaCO
35, MnSO
4.4H
2O 0.54, peptone 2, KH
2PO
45, NaCl 0.15, analysis for soybean powder 10, mealy potato 3, starch 5, CaCO
33, agar 5, ferrous sulfate 0.5, copper sulphate 0.6, manganese sulphate 0.2, chlormequat 0.001, sodium humate 0.008, alar-85 0.001.
5, other agricultural chemicals, 0.5 kilogram of β-asarone, 0.5 kilogram of moulting hormone, 0.5 kilogram of juvenile hormone, 0.5 kilogram of sex pheromone, 0.5 kilogram of food refusal hydrazine, 0.5 kilogram of citronella oil, 1 kilogram of pyrethrin, 1 kilogram of nereistoxin, 1 kilogram of limonoid amount to 6 kilograms.
6, described adsorbent is put into agitator for 190 kilograms, add 60 kilograms of described bacterium liquid again, 5 kilograms of nutrient solutions, 6 kilograms in agricultural chemicals.Then, start agitator, stirred 5-15 minute,, just make 261 kilograms of disease and pest control agents that contain multiple microorganism until stirring with 50-250 rev/min speed.
Adopt the disease and pest control agent that this kind contains multiple microorganism to test, use in the process of insects such as the open-air and indoor cockroach of control, mosquitos and flies, the incidence of insects such as cockroach, mosquitos and flies is obviously reduced, lethality is more than 95%.The lethality of insects such as aphid, plant hopper, leafhopper, aleyrodid, liriomyza bryoniae, weevil is more than 95%.The above-mentioned insect that chemical pesticide is developed immunity to drugs, this product has special efficacy.
Quality, the control efficiency that contains the disease and pest control agent of multiple microorganism of the present invention can reach and surpass similar products like and related standards.
Using method and the usage amount that contains the disease and pest control agent of multiple microorganism of the present invention:
Using method 1 of the present invention (using when being main) with insect pest:
This product is novel efficient, broad spectrum activity biological insecticides, contains internationally recognized multiple special microorganism, and is only effective to the target disease and insect.The disease and insect that chemical pesticide is developed immunity to drugs, this product has special efficacy.
This product have special efficacy, long-acting, access times are few, expense is low, be difficult for developing immunity to drugs, characteristics free from environmental pollution and that the natural enemy (bird, the frog, beast) of people, domestic animals and fowls and insect is had no side effect.It is the ideal product of " ecological medication ".
Main control: aphid, plant hopper, leafhopper, thrips, aleyrodid, liriomyza bryoniae, coccid, weevil, stinkbug silk worm, cabbage caterpillar, cockroach, mosquitos and flies etc.
Using method:
1. spray: this product is converted water 30-50 litre, be sprayed on equably on the branches and leaves of plant, also can water in plant roots.
2. seed dressing: this product is mixed and stirred equably on seed, be with mixing with broadcasting.
Usage amount: 1 bottle (20 gram) can be used for 1 mu of ground.
Points for attention:
1. can not mix use with bactericide and acidity, alkaline matter.
2. forbid edible or feeding, away from breed silkworms, bee farm institute.
3. use soap and water to clean exposure portion.Irritated and malaise symptoms person are arranged, send treatment to hospital.
4. low temperature or shady and cool dry place are deposited in sealing, and the term of validity is 2 years.
Using method 2 of the present invention (damage by disease and insect all uses more for a long time):
This product is novel efficient, the biological disease and pest control agent of broad spectrum activity, contains internationally recognized multiple special microorganism, and is only effective to the target disease and insect.The disease and insect that chemical pesticide is developed immunity to drugs, this product has special efficacy.
This product have special efficacy, long-acting, access times are few, expense is low, be difficult for developing immunity to drugs, characteristics free from environmental pollution and that the natural enemy (bird, the frog, beast) of people, domestic animals and fowls and insect is had no side effect.It is the ideal product of " ecological medication ".
Main control: root rot, take-all, rust, rice blast, banded sclerotial blight, multiple damage by disease and insect such as cotton seedling blight, aphid, plant hopper, leafhopper, liriomyza bryoniae, weevil, stinkbug silk worm, cabbage caterpillar, cockroach.
Using method:
1. spray: this product is converted water 30-50 litre, be sprayed on equably on the branches and leaves of plant, also can water in plant roots.
2. seed dressing: this product is mixed and stirred equably on seed, be with mixing with broadcasting.
Usage amount: 1 bottle (20 gram) is used for 1 mu of ground.
Points for attention:
1. can not mix use with bactericide and acidity, alkaline matter.
2. forbid edible or feeding, away from breed silkworms, bee farm institute.
3. use soap and water to clean exposure portion.Irritated and malaise symptoms person are arranged, send treatment to hospital.
4. low temperature or shady and cool dry place are deposited in sealing, and the term of validity is 2 years.
Using method 3 of the present invention (in the open air with insects such as indoor control cockroach, mosquitos and flies time use):
This product is novel efficient, broad spectrum activity biological insecticides, contains internationally recognized multiple special microorganism, and is only effective to target pest.The insect that chemical pesticide is developed immunity to drugs, this product has special efficacy.
This product have special efficacy, long-acting, access times are few, expense is low, be difficult for developing immunity to drugs, free from environmental pollution, and characteristics that people, pet, domestic animals and fowls are had no side effect.It is the ideal product of " ecological medication ".
Using method: spray or be placed on the place that cockroach, mosquitos and flies are are often come in and gone out, look for food and hide.
1. spray: will add clear water in the watering can, rock evenly, spray.
2. dusting: every bag of medicinal powder is divided into 3-5 part, places respectively.
3. pharmaceutical chemistry: medicinal powder is admixed in 2-3 bread (steamed bun, the rice etc.) chip doubly, be divided into 5-8 part, place respectively.
Usage amount: 1 bag (5 gram) is used for 20 square metres.
Points for attention:
1. can not mix use with bactericide and acidity, alkaline matter.
2. do not store up altogether with food, forbid edible or feeding, away from breed silkworms, bee farm institute.
3. use soap and water to clean exposure portion, irritated and malaise symptoms person are arranged, send treatment to hospital.
4. shady and cool dry place is deposited in sealing, and the term of validity is 2 years.
Claims (9)
1, a kind of disease and pest control agent that contains multiple microorganism is characterized in that it comprises following component:
1) microbial inoculum, it is made up of described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum and described beneficial microbe bacterium liquid, and described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum is at least a bacterium liquid that is selected from bacillus thuringiensis bacterium liquid, pseudomonas fluorescens bacterium liquid, streptomycete bacterium liquid, white muscardine fungi bacterium liquid, green muscardine fungus bacterium liquid and/or the paecilomycerol bacterium liquid; Described beneficial microbe bacterium liquid is at least a bacterium liquid that is selected from azotobacteria bacterial liquid, phosphobacteria bacterium liquid and/or the potassium bacterium bacterium liquid;
The bacterial classification of wherein said biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum is respectively bacillus thuringiensis (Bacillusthuringiensis subsp AS 1.785,1.995,1.897,1.961,1.1754, ACCC 10016,10024,10025,10028,10033,10061), pseudomonas fluorescens (Pseudmonasfluorescens AS 1.33,1.55,1.867, and ACCC 10040); Light day blue streptomycete (strptomyces coerulescens AS 4.4653 of streptomyces, 4.680,4.929, ACCC 40010), light gray streptomycete (Streptomyces griseolus var AS 4.456,4.507, ACCC 40075), thorn spore streptomyces hygroscopicus (Streptomyces hygrospinosus Yan et Tao ACCC 40015,40016,40017,40033,40051,40068,40071), streptomyces hygroscopicus (Streptomyces hygroospinosusvar AS 4.168,4.911,4.1026,4.1004 ACCC 40034,40051,40064), celebrate rich streptomycete (Streptomyces qingfengmyceticus Saalmsipp AS 4.1036, ACCC 40078), streptomyces rimosus (Streptomyces rimosus Sobin et al AS 4.748, ACCC 40079); Beauveria beauveria bassiana (Beauveria bassiana Vuillemin AS 3.2055,3.4270,3.4428, ACCC30006,30107,30113), white muscardine fungi (Beauveria sp AS 3.470,3.3474,3.3475,3.3576), powdery white muscardine fungi (Beauveria velatl Samson AS 3.4429); Green muscardine fungus genus blastomyces green muscardine fungus (Metarhizium album Petch ACCC 30198), Metarhizium anisopliae (Metarhiziumanisopliae ACCC 30101,30102,30104,30131,30194), brown green muscardine fungus (Metarhizium brunneum Petch ACCC 30200), post spore green muscardine fungus (Metarhiziumeylindrosporae Chen et Guo ACCC 30114), emerald green green muscardine fungus (Metarhizium iadiniChen ACCC 30124), big spore green muscardine fungus (Metarhizium maiarosporae ACCC 30187,30195), flat husky green muscardine fungus (Metarhizium pingshaense Chen et Guo ACCC 30105); Paecilomyces varioti Pseudomonas powder paecilomycerol (Paecilomyces farinosus ACCC 30190, CFCC 5079), paecilomycerol (Paecilomyces sp AS 3.2762,3.3728, ACCC 30147) bacterial strain;
The bacterial classification of wherein said beneficial microbe bacterium liquid is respectively azotobacter blown-ball Azotobacter (Azotobacterchroococcum ACCC 10001,10002,10006, AS 1.178,1.212,1.222), Wei Nielande azotobacter (Azotobacter Vinelandii ACCC 10087,10088, AS 1.207,1.824,1.1005,1.1649), rhizobium of bean (Rhizobium japonicum ACCC 15005,15018,15067, AS 1.174,1.1826,1.1828), rice Flavobacterium (Flovobacterium oryzae spnovACCC 10051,10052); Bacillus bacillus megaterium (Bacillus megaterium ACCC10008,10010,10011, AS 1.127,1.515,1.941), Bacillus cercus (Bacilluscereus AS 1.182,1.1626), colloid bacillus cereus (Bacillus ACCC 10012, AS 1.231) bacterial strain;
2) nutrient solution, it is at least a nutrients that is selected from bacterial cultures, trace compound or the plant growth regulator;
3) adsorbent;
4) agricultural chemicals, it is to be selected from least a agricultural chemicals in botanical pesticide, animal sources agricultural chemicals, mineral sources agricultural chemicals or the synthetic pesticide;
It is as follows that described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum, beneficial microbe bacterium liquid, nutrient solution, adsorbent and other agricultural chemicals are formed (in weight portion):
Described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum 20-35 part;
Described beneficial microbe bacterium liquid 5-15 part;
Described nutrient solution 5-20 part;
Described adsorbent 60-75 part;
Described agricultural chemicals 1-25 part.
2, method according to claim 1 is characterized in that: it is as follows that described microbial inoculum is formed (in weight portion):
Described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum:
Bacillus thuringiensis bacterium liquid 1-30 part;
Pseudomonas fluorescens bacterium liquid 0.1-5 part;
Streptomycete bacterium liquid 1-40 part;
White muscardine fungi bacterium liquid 1-20 part;
Green muscardine fungus bacterium liquid 1-30 part;
Paecilomycerol bacterium liquid 1-5 part.
Described beneficial microbe bacterium liquid:
Azotobacteria bacterial liquid 1-15 part;
Phosphobacteria bacterium liquid 1-10 part;
Potassium bacterium bacterium liquid 1-10 part.
3, method according to claim 1 is characterized in that: it is 30-50 more than hundred million/milliliter or 10,000,000,000/gram of spore that described biological and ecological methods to prevent plant disease, pests, and erosion microbial inoculum contains effective bacterium number, and beneficial microbe bacterium liquid contains effective bacterium and counts 30-50 more than hundred million/milliliter.
4, method according to claim 1 is characterized in that: it is as follows that described bacterial cultures, trace compound and plant growth regulator are formed (in weight portion):
Described bacterial cultures 25-70 part;
Described trace compound 5-35 part;
Described plant growth regulator 1-15 part.
5, method according to claim 1 is characterized in that: described bacterial cultures is to be selected from glucose, sucrose, peptone, meat extract, glycerine, agar, analysis for soybean powder, mealy potato, starch, CaCO
3, MnSO
4.4H
2O, KH
2PO
4, NaCl, yeast extract, ammonium molybdate, ironic citrate, K
2HPO
4, MgSO
4.7H
2O, FeSO
4.7H
2At least a culture in O or the fused calcium magnesium phosphate.
6, method according to claim 1 is characterized in that: described trace compound is at least a compound that is selected from borax, zinc sulphate, ferrous sulfate, copper sulphate, manganese sulphate, ammonium molybdate, cobalt oxide, titanium oxide, selenium oxide or the potassium iodide.
7, method according to claim 1, it is characterized in that: described plant growth regulator is to be selected from heteroauxin, 2, at least a plant growth regulator in 4-D, volume increase element, methyl, sevin, gibberellin, zeatin, kinetin, di-phenylurea, triacontanol, brassin lactones, chlormequat, sodium humate, alar-85 or the paclobutrazol.
8, method according to claim 1 is characterized in that: described adsorbent is at least a adsorbent that is selected from kaolin, potter's clay, clay, diatomite, talcum powder, white carbon or the pyrophyllite.
9, method according to claim 1 is characterized in that:
Described botanical pesticide is to be selected from rotenone, nicotine, pyrethrin, anabasine, jervine, fennel artemisin, sodium alginate, gossylure, juvenile hormone, nimbin, limonoid, caryophyllus oil or citronella oil, β-asarone, sesamin, piperonyl butoxide, heteroauxin, ethene, the basic element of cell division, gibberellin, abscisic acid, brassin lactones, zeatin, 2, at least a agricultural chemicals in 4-D or the methyl;
Described animal sources agricultural chemicals be selected from nereistoxin, Manda draws at least a agricultural chemicals in toxin, cantharidin, moulting hormone, brain hormone, juvenile hormone or the sex pheromone;
Described mineral sources agricultural chemicals is selected from least a agricultural chemicals in zinc phosphide, aluminum phosphate or the Kocide SD;
Described synthetic pesticide is to be selected from carbofuran, Aldicarb, Aphox, Bassa, unden, decis, flucythrinate, brofluthrinate, ether chrysanthemum ester, permethrin, diflubenzuron, the young urea that goes out, Teflubenzuron, Buprofezin, dimehypo, desinsection list, cartap, polythiane, food refusal hydrazine, triaguron, arsenic worm quinoline, four mite piperazines, to reach at least a agricultural chemicals in mite ketone, azacyclotin, propargite, ethoprop, Aldicarb or the dazomet.
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