CN109362791A - A kind of biological insecticides and preparation method thereof for preventing and treating longicorn insect pest - Google Patents
A kind of biological insecticides and preparation method thereof for preventing and treating longicorn insect pest Download PDFInfo
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- CN109362791A CN109362791A CN201811491920.2A CN201811491920A CN109362791A CN 109362791 A CN109362791 A CN 109362791A CN 201811491920 A CN201811491920 A CN 201811491920A CN 109362791 A CN109362791 A CN 109362791A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
Longicorn is safely, effectively killed, without drug resistance, the biological insecticides for avoiding environment from suffering contaminated prevention and treatment longicorn insect pest and preparation method thereof the invention discloses a kind of.The biological insecticides of prevention and treatment longicorn insect pest are made of the component of following mass fraction: 75~85 parts of beauveria bassiana culture solution, 40~50 parts of 500 times of dilutions of nimbin solution, 2~4 parts of emulsifier, 1~3 part of dehydrated alcohol.Preparation method is: 500 times of dilutions, emulsifier, dehydrated alcohols of above-mentioned beauveria bassiana culture solution, nimbin solution are uniformly mixed.It the composite can be widely applied to pest and disease control of fruit trees field.
Description
Technical field
The present invention relates to a kind of biological insecticides for preventing and treating longicorn insect pest.In addition, the invention further relates to a kind of prevention and treatment longicorns
The process for preparing bio-insecticide of insect pest.
Background technique
Longicorn is the general name of Polyphaga Cerambycidae, is main on production of forestry, arable farming and construction timber
One of pest, and the primary pest for endangering orchard.Longicorn is biting mouthparts, and palate is strong, gnaws that ability is strong, and adult gnaws
Newborn branch, bark, limb, young fruit, achievement can leave strip scar after gnawing.Serious, branch bark is gnawed light, is made
It is withered at fruiting branch frangibility.And adult can lay eggs at limb gap and in bark, and the larva after egg hatching can be along wooden
Portion, marrow eat into downwards food, up to root, can lead to that plant strain growth is bad, and tree vigo(u)r early ageing seriously affects achievement yield, when serious
Complete stool can be caused withered.Adult gnaws everywhere, and oviposition, larva moves in inside trees, is not easy to prevent and treat.Main prevention and treatment is done at present
Method is chemical prevention, although killing rate is big, control efficiency is good, is easy contaminated soil, air, fruit, chemical residual amount is big, shadow
Edible health is rung, while being easy that longicorn is made to develop drug resistance.There is a method in which trap-tree need to be planted by being traped by trap-tree, but its
Heavy workload, effect are unobvious.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and provide one kind safely, effectively to kill day
Ox, without drug resistance, avoid environment by it is contaminated prevention and treatment longicorn insect pest biological insecticides.
The present invention also provides a kind of process for preparing bio-insecticide for preventing and treating longicorn insect pest.
Technical solution used by the biological insecticides of prevention and treatment longicorn insect pest of the invention is: prevention and treatment longicorn worm of the invention
Harmful biological insecticides are made of the component of following mass fraction: 75~85 parts of beauveria bassiana culture solution, nimbin solution
500 times 40~50 parts of dilution, 2~4 parts of emulsifier, 1~3 part of dehydrated alcohol.
Further, the biological insecticides of prevention and treatment longicorn insect pest of the invention are made of the component of following mass fraction: ball spore
80 parts of muscardine culture solution, 45 parts of 500 times of dilutions of nimbin solution, 3 parts of emulsifier, 2 parts of dehydrated alcohol.
The concentration of beauveria bassiana spore is 100~120/ml in the beauveria bassiana culture solution.
The emulsifier uses polysorbas20.
The concentration of nimbin is 0.3%~0.5% in the nimbin solution.
The nimbin solution uses neem tree skin and/or root skin, and water, sodium chloride is added, is made after decoction filtering.
Technical solution used by the process for preparing bio-insecticide of prevention and treatment longicorn insect pest of the invention is: of the invention
The biological insecticides of prevention and treatment longicorn insect pest are made of the component of following mass fraction: 75~85 parts of beauveria bassiana culture solution, print
40~50 parts of 500 times of dilutions of chinaberry element solution, 2~4 parts of emulsifier, 1~3 part of dehydrated alcohol, preparation method includes following step
It is rapid:
(1) beauveria bassiana is inoculated with, cultivated, obtain beauveria bassiana culture solution, beauveria bassiana in culture solution
The concentration of spore is 100~120/ml；
(2) bark and/or root skin of print chinaberry are taken, water, sodium chloride is added, nimbin solution for standby is made after decoction filtering,
The concentration of nimbin is 0.3%~0.5% in nimbin solution；
(3) emulsifier is taken, it is spare；
(4) dehydrated alcohol is taken, it is spare；
(5) the nimbin solution that step (2) obtain is diluted with water to 500 times of dilutions, dilution and step (1) is taken
The emulsifier of the beauveria bassiana culture solution, step (3) acquirement that obtain, the dehydrated alcohol of step (4) acquirement are uniformly mixed.
Further, the biological insecticides of prevention and treatment longicorn insect pest of the invention are made of the component of following mass fraction: ball spore
80 parts of muscardine culture solution, 45 parts of 500 times of dilutions of nimbin solution, 3 parts of emulsifier, 2 parts of dehydrated alcohol.
The emulsifier uses polysorbas20.
In step (2), bark and/or root skin and the quality proportioning of water, sodium chloride are 100:200:1.
In step (2), bark and/or root skin are first boiled by fire after water, sodium chloride is added, then it is cooked by slow fire 60~
90min。
The beneficial effects of the present invention are: the biological insecticides of prevention and treatment longicorn insect pest of the invention are closed using specific ratio
The beauveria bassiana culture solution and nimbin solution of system prevent and treat the insect pest of peach garden longicorn, beauveria bassiana as main function raw material
It is a kind of ascomycetous entomogenous fungi, the high cryptogam of muscardine is non-toxic and tasteless, non-environmental-pollution, has persistent infection to pest
Power, pest can continuously disseminate propagation once infection, and no agriculture is residual, will not cause to appoint harvesting product immediately after applying muscardine
What pesticide residue, non-resistant, for muscardine by lethal with the contact infection of the body wall of pest under field conditions (factors), pest will not be right
It generates any resistance, uses year after year, and effect is higher and higher instead, and beauveria bassiana contains living body fungi and spore, applies field
Between after, by suitable temperature, humidity, flourish can be continued, enhance insecticidal effect, there is regrowth, be different from changing
What medicine of learning to farm was not distinguished between the enemy and ourselves kills beneficial insect, pest total number, and muscardine specificity is strong, to non-target organism such as ladybug, Chrysopa and
Eating the beneficial insects such as aphid horsefly influences smaller, and with highly selective, therefore muscardine is a kind of environment friendly agricultural, ideal pesticide, both can be with
Longicorn insect pest is safely and effectively controlled, and can protect the places such as orchard, ecological environment is avoided to destroy by pollution；Printing chinaberry is one
The title of kind fast-growing deciduous tree, seed and bark can all be used as medicine, and be a kind of ideal pesticide plant, and nimbin is a kind of height
The citrin of oxidation, there are many similar functional groups for band, and seven kinds of active ingredients of AZ-A to AZ-G were once isolated from neem seed
Object, wherein A is main insecticidal constituent, print the ingredients such as nimbin for extracting in chinaberry fruit be a kind of wide spectrum, efficiently, low toxicity, easily
Degradation, noresidue to pest have repelling and killing efficacy and the not no insecticide of drug resistance；The present invention by neem tree skin and/or
Nimbin is extracted in root skin, at low cost, effect is good；It being practiced and is tested, the present invention reaches 95% or more to the eradicative rate of longicorn,
It is that one kind safely, effectively kills longicorn, without drug resistance, the Biocidal for avoiding environment from suffering contaminated prevention and treatment longicorn insect pest
Agent.
Specific embodiment
The biological insecticides of prevention and treatment longicorn insect pest of the invention are made of the component of following mass fraction: beauveria bassiana training
75~85 parts of nutrient solution, 40~50 parts of 500 times of dilutions of nimbin solution, 2~4 parts of emulsifier, 1~3 part of dehydrated alcohol.
Specific to the present embodiment, the biological insecticides of the prevention and treatment longicorn insect pest are made of the component of following mass fraction:
80 parts of beauveria bassiana culture solution, 45 parts of 500 times of dilutions of nimbin solution, 3 parts of emulsifier, 2 parts of dehydrated alcohol；Wherein,
The concentration of beauveria bassiana spore is 100~120/ml in the beauveria bassiana culture solution, and the emulsifier uses tween
20, the concentration of nimbin is 0.3%~0.5% in the nimbin solution, the nimbin solution using neem tree skin and/or
Root skin is added water, sodium chloride, is made after decoction filtering.
The present embodiment prevention and treatment longicorn insect pest process for preparing bio-insecticide the following steps are included:
(1) beauveria bassiana is inoculated with, cultivated, obtain beauveria bassiana culture solution, beauveria bassiana in culture solution
The concentration of spore is 100~120/ml；
(2) bark and/or root skin of print chinaberry are taken, water, sodium chloride, the quality of bark and/or root skin and water, sodium chloride is added
Proportion is 100:200:1, is first boiled by fire, is then cooked by slow fire 60~90min, it is standby that nimbin solution is made after decoction filtering
With the concentration of nimbin is 0.3%~0.5% in nimbin solution；
(3) emulsifier is taken, spare, the emulsifier uses polysorbas20；
(4) dehydrated alcohol is taken, it is spare；
(5) the nimbin solution that step (2) obtain is diluted with water to 500 times of dilutions, dilution and step (1) is taken
Beauveria bassiana culture solution, step (3) obtain emulsifier, step (4) obtain dehydrated alcohol according to above-mentioned mass parts
Several proportions is uniformly mixed.
The application method of the biological insecticides of prevention and treatment longicorn insect pest of the invention is exemplified below:
(A) prepared biological insecticides spray fruit tree especially tender leaf, newborn branch；
(B) prepared biological insecticides liftoff 1 meter of position below is especially to trunks of fruit trees to brush；
(C) prepared biological insecticides are irrigated trunk hole or punching；If punching, one tree should not be more than 10
It is a；
(D) prepared biological insecticides are irrigated trunk hole or punching；It is mixed with soil, trunk hole is carried out
It blocks；
(E) prepared biological insecticides are irrigated trunk hole or punching；It is mixed with soil, trunk is applied
Brush.
By the biological insecticides of the prevention and treatment longicorn insect pest of above-described embodiment, in the village Jiashan of Zhejiang Xian Yao, whitefish swings 110 mu certainly
There is yellow peach garden to be implemented, specific implementation process are as follows:
(1) after roundheaded borer comes into play, (C) and (D) step in above-mentioned application method citing is carried out March；
(2) May, longicorn start to lay eggs, and carry out (B) and (E) step in above-mentioned application method citing；
(3) July~August part carries out (A) and (C) step in above-mentioned application method citing.
Above procedure, every 5 days are a cycle, check medication effect, and repeat to be administered.
Above embodiments are only for the purpose of illustration to be used, and is not construed as limiting the scope of the present invention.It is not departing from
All using description of the invention and the various equivalence changes done in the case where inventive concept, reason is the same as being contained in the present invention
Scope of the claims in.
The biological insecticides of prevention and treatment longicorn insect pest of the invention use the beauveria bassiana culture of specific ratio relationship
As the prevention and treatment peach garden longicorn insect pest of main function raw material, beauveria bassiana is a kind of ascomycetous worm for liquid and nimbin solution
Raw fungi, the high cryptogam of muscardine is non-toxic and tasteless, non-environmental-pollution, has persistent infection power to pest, pest can connect once infection
Continuous dip dyeing is propagated, and no agriculture is residual, will not cause any pesticide residue, nonreactive harvesting product immediately after applying muscardine
Property, for muscardine by lethal with the contact infection of the body wall of pest under field conditions (factors), pest will not generate any resistance to it, even
Year uses, and effect is higher and higher instead, and beauveria bassiana contains living body fungi and spore, after applying field, by suitable temperature
Degree, humidity can continue flourish, enhance insecticidal effect, have regrowth, incite somebody to action different from what chemical pesticide was not distinguished between the enemy and ourselves
Beneficial insect, the poisoning of pest total number, muscardine specificity is strong, on non-target organism such as ladybug, Chrysopa and food aphid horsefly etc. beneficial insects influence compared with
Small, with highly selective, therefore muscardine is a kind of environment friendly agricultural, ideal pesticide, both can safely and effectively control longicorn
Insect pest, and can protect the places such as orchard, avoid ecological environment from destroying by pollution；Print chinaberry is a kind of name of fast-growing deciduous tree
Claim, it is a kind of ideal pesticide plant that seed and bark, which can all be used as medicine, and nimbin is a kind of highly oxidized citrin, is had
Many similar functional groups once isolated seven kinds of reactive compounds of AZ-A to AZ-G from neem seed, and wherein A is main kills
Worm ingredient, print the ingredients such as nimbin for extracting in chinaberry fruit be a kind of wide spectrum, it is efficient, less toxic, degradable, noresidue to pest
With the repelling and killing efficacy and not insecticide of drug resistance；The present invention by extracting nimbin in neem tree skin and/or root skin, at
This is low, and effect is good；It is practiced and is tested, the present invention reaches 95% or more to the eradicative rate of longicorn, is that one kind is safely, effectively killed
Longicorn, without drug resistance, avoid environment by it is contaminated prevention and treatment longicorn insect pest biological insecticides.
It the composite can be widely applied to pest and disease control of fruit trees field.
Claims (10)
1. a kind of biological insecticides for preventing and treating longicorn insect pest, it is characterised in that: be made of the component of following mass fraction: ball spore is white
75~85 parts of stiff bacteria culture fluid, 40~50 parts of 500 times of dilutions of nimbin solution, 2~4 parts of emulsifier, dehydrated alcohol 1~3
Part.
2. the biological insecticides of prevention and treatment longicorn insect pest according to claim 1, it is characterised in that: by following mass fraction
Component is made: 80 parts of beauveria bassiana culture solution, 45 parts of 500 times of dilutions of nimbin solution, and 3 parts of emulsifier, dehydrated alcohol 2
Part.
3. the biological insecticides of prevention and treatment longicorn insect pest according to claim 1 or 2, it is characterised in that: the white deadlock of ball spore
The concentration of beauveria bassiana spore is 100~120/ml in bacteria culture fluid.
4. the biological insecticides of prevention and treatment longicorn insect pest according to claim 1 or 2, it is characterised in that: the emulsifier is adopted
Use polysorbas20.
5. the biological insecticides of prevention and treatment longicorn insect pest according to claim 1 or 2, it is characterised in that: the nimbin is molten
The concentration of nimbin is 0.3%~0.5% in liquid.
6. the biological insecticides of prevention and treatment longicorn insect pest according to claim 5, it is characterised in that: the nimbin solution is adopted
With neem tree skin and/or root skin, water, sodium chloride is added, is made after decoction filtering.
7. a kind of process for preparing bio-insecticide of prevention and treatment longicorn insect pest of any of claims 1 or 2, it is characterised in that: packet
Include following steps:
(1) beauveria bassiana is inoculated with, cultivated, obtain beauveria bassiana culture solution, beauveria bassiana spore in culture solution
Concentration be 100~120/ml；
(2) bark and/or root skin of print chinaberry are taken, water, sodium chloride is added, nimbin solution for standby is made after decoction filtering, prints chinaberry
The concentration of nimbin is 0.3%~0.5% in plain solution；
(3) emulsifier is taken, it is spare；
(4) dehydrated alcohol is taken, it is spare；
(5) the nimbin solution that step (2) obtain is diluted with water to 500 times of dilutions, dilution and step (1) is obtained
The dehydrated alcohol of emulsifier, step (4) acquirement that beauveria bassiana culture solution, step (3) obtain is uniformly mixed.
8. the process for preparing bio-insecticide of prevention and treatment longicorn insect pest according to claim 7, it is characterised in that: the cream
Agent uses polysorbas20.
9. the process for preparing bio-insecticide of prevention and treatment longicorn insect pest according to claim 7, it is characterised in that: step
(2) in, bark and/or root skin and the quality proportioning of water, sodium chloride are 100:200:1.
10. the process for preparing bio-insecticide of prevention and treatment longicorn insect pest according to claim 7, it is characterised in that: step
(2) it in, is first boiled by fire after bark and/or root skin addition water, sodium chloride, is then cooked by slow fire 60~90min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811491920.2A CN109362791A (en) | 2018-12-07 | 2018-12-07 | A kind of biological insecticides and preparation method thereof for preventing and treating longicorn insect pest |
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| CN201811491920.2A CN109362791A (en) | 2018-12-07 | 2018-12-07 | A kind of biological insecticides and preparation method thereof for preventing and treating longicorn insect pest |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113812291A (en) * | 2021-09-17 | 2021-12-21 | 山东夏禾绿色防控研究院有限公司 | Biological control method for trunk borers and application thereof |
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Application publication date: 20190222 |