CN102657164A - Prevention and treatment method for peach fruit moths as well as special contact solution and fumigation solution - Google Patents
Prevention and treatment method for peach fruit moths as well as special contact solution and fumigation solution Download PDFInfo
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- CN102657164A CN102657164A CN2012101669606A CN201210166960A CN102657164A CN 102657164 A CN102657164 A CN 102657164A CN 2012101669606 A CN2012101669606 A CN 2012101669606A CN 201210166960 A CN201210166960 A CN 201210166960A CN 102657164 A CN102657164 A CN 102657164A
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Abstract
The invention relates to a prevention and treatment method for peach fruit moths. The prevention and treatment method is characterized by comprising the following steps of: (1) forming contact solution by trans-anethole and alcohol (having a volume concentration of 98%) in a volume ratio of 1: 100, wherein the contact solution is used for killing the moths by means of contact before the bags of fruits are released in June, July and August; and (2) forming fumigation solution by the trans-anethole and the alcohol (having a volume concentration of 98%) in a volume ratio of 1: 50, wherein the fumigation solution is used for killing the moths by means of fumigation after the bags of fruits are released and before the fruits are taken off from trees. The prevention and treatment method disclosed by the invention is low in toxicity, less in residue, strong in selectivity, and safe to natural enemy, can not destroy ecological environment, can avoid the problem of the resistance generated by diseases and insect pests; and most crucially, human security is highly guaranteed on the premise of no reduction on killing and treatment effects.
Description
Technical field
The present invention relates to agricultural and use insecticide, prevent and treat composition and the method for using of peach fruit borer specifically.
Background technology
Peach fruit borer (Crposina sasakiiMatsmura), the abbreviation peach is little, and popular name sweetened bean paste stuffing, small holes caused by worms, jaundice belong to Lepidoptera moth fruit moth section.Being distributed in northeast, North China, northwest, East China, Central China at home, wherein mainly is the north and northwestward apple, pears and jujube producing region, only is distributed in Japan, Korea and Muscovite the Far East Area abroad.Be maximum, the most general heart-eating worm class pest of generation area of harm in the northern China production of fruit trees, its feeding habits are very assorted, can endanger the fruit of more than 10 kind of fruit trees such as apple, jujube, pears.The 1950's, this worm is generally taken place in China, and it is heavier to cause harm, and is once classified as one of agricultural ten big damage by disease and insect.Simultaneously, because killed fruit quality descends, reduced commodity value.Along with the development of agricultural chemicals, the effective control of once this worm being carried out many high-toxic pesticide kinds appearred; But chemical pesticide contaminated soil, water body, atmosphere are residual in a large number in environment; Enrichment transmission in human biological chain, along with human diet gets in the body, the safety that serious threat is human.
The Along with people's living standard improves in recent years; To fruit quality require increasingly highly, country is to the restriction of high-toxic pesticide in addition, many high poison, persistent pesticide are under an embargo successively; It is on the rise to cause peach fruit borer to be caused harm, and has brought enormous economic loss to the orchard worker.Current; The requirement of human consumption's agricultural product and food has forwarded to from resolving adequate food and clothing takes care, improves healthy requirements at the higher level; Therefore the consumer to pure natural, pollution-free, high-quality, do not use any chemical substances such as fertilizer and pesticide, somatotropin, do not adopt pollution-free food, the pollution-free food of gene engineering and irradiation technique to show strong demand desire yet.
At present, the biological control to peach fruit borer mainly contains:
1. parasitic wasp; The little Chelonus of Zhang Shi, Chinese tooth leg ichneumon wasp, the little tooth leg of peach ichneumon wasp; 2. biologic product: the young urea that goes out, AVM, the little spirit of peach etc.; 3. entomopathogen: insect pathogenic fungus, insect disease indigenous bacteria, entomopathogenic nematode; 4. sex attractant.
Utilizing parasitic wasp control peach the highest little parasitic rate is 16.45%, and also has a lot of seeds not find parasitic wasp so far.Utilize entomopathogen desinsection rapid speed; The host there are stronger search capability and selectivity; Especially dwelling property of soil and borer pest and other disguised insect there is special preventive effect; But the method very easily receives the influence of environmental condition, and the change of conditions such as temperature on every side, humidity, illumination all can change control efficiency, can influence the quality of fruit simultaneously.
At present most popular is the gyplure control, and still, sex attractant just can attract male worm, and can not attract female worm, and have male worm escape lure and with female worm mating, in case the mating success, gyplure is with ineffective.
Therefore, the method for preventing and treating of being badly in need of effectively at present and can not polluting fruit and environment is to satisfy user demand.
Summary of the invention
The object of the invention just provides a kind of peach fruit borer and prevents and treats method and special use tag liquid and fumigation liquid, solving in the prior art or unfavorable to the peach fruit borer control, or the double-barreled question that fruit or environment are polluted.
The objective of the invention is to realize like this:
Peach fruit borer prevents and treats method:
1. trans-anethole and alcohol (volumetric concentration 98%) were formed the liquid of tagging in 1: 100 by volume, be used for 6,7, utilize the means desinsection of tagging August;
2. trans-anethole and alcohol (volumetric concentration 98%) were formed fumigation liquid in 1: 50 by volume, be used for separating the bag back, utilizing stifling means desinsection before the tree down at fruit.
Said stifling means are the uncovered bottle of 10cm for said fumigation liquid being placed bore, hang four in every peripheral evenly cloth of tree.
Tagging 1. is arranged in 6,7, August fruit separate before the bag and to carry out once in every month.
The liquid of tagging of the present invention was made up of trans-anethole and alcohol (volumetric concentration 98%) in 1: 100 by volume.
Fumigation liquid of the present invention was made up of trans-anethole and alcohol (volumetric concentration 98%) in 1: 50 by volume.
The method of preventing and treating of the present invention is through the selection to raw material, proportioning; Process plant insecticide; In conjunction with selection to desinsection opportunity and desinsection mode; Taken into account desinsection cost, insecticide efficiency and fruit and environmental pollution several respects, the insecticide that the plant resource raw material is processed successfully is used for peach fruit borer is carried out high-efficiency prevention and control.Method of the present invention at first uses the means of tagging that adult is controlled extremely during fruit jacket bag, owing to selected suitable proportioning and suitable desinsection opportunity; Insecticide efficiency is high; Both can kill by just large batch of adult, and laid eggs and fruit is caused infringement separating a bag back large batch of adult avoiding in the future, and can not pollute because of the existence that bagging is arranged again fruit; And control respond well extremely; Thereby fumigate insect protected behind the bag and lay the first stone for separating, make that separating the bag back becomes possibility through stifling mode insect protected, and then the possibility of means of effectively having avoided tagging to fruit contamination.Of the present invention prevent and treat the method low toxicity, residual less, selectivity is strong, to natural enemies security and can not destroy ecotope, the problem that damage by disease and insect produces resistance can not appear yet, most critical be not reduce under the prerequisite of controlling effect extremely, highly guaranteeing human security.
Embodiment
The liquid of tagging of the present invention was made up of trans-anethole and alcohol (volumetric concentration 98%) in 1: 100 by volume.
Fumigation liquid of the present invention was made up of trans-anethole and alcohol (volumetric concentration 98%) in 1: 50 by volume.
The peach fruit borer method of preventing and treating of the present invention comprises two parts; It at first was 1. stage; Trans-anethole and alcohol (volumetric concentration 98%) were formed the liquid of tagging in 1: 100 by volume; Be used for 6,7, utilize the means desinsection of tagging August, tag be arranged in 6,7, August fruit separate before the bag and to carry out once getting final product in every month.Be the 2. stage then, trans-anethole and alcohol (volumetric concentration 98%) were formed fumigation liquid in 1: 50 by volume, be used for separating the bag back, utilizing before the tree and fumigate the means desinsection down at fruit.When stifling, it is the uncovered bottle of 10cm that said fumigation liquid is placed bore, hangs four in every peripheral evenly cloth of tree, can play repellent action.
Under the laboratory condition, trans-anethole is to the test of peach fruit borer insecticidal effect
In the embodiments of the invention, peach fruit borer is raised condition:
In climatic cabinate, raise the climatic cabinate condition: keep temperature (23 ± 1) ℃, RH (80 ± 7) %, photoperiod 15h ︰ 9h (L:D).
The raising of peach fruit borer is carried out in vial, and with the Cans that every pair of adult is put into, seal with wet gauze on cage top, and 1 of bottleneck suspension contains cotton balls and 2～3 strips of 10% hydromel, the filter paper (8cm * 2cm) supply it to lay eggs of hair stubble.Then, dependent insect cage is placed in the climatic cabinate.Collect the ovum card every day, when ovum just blackhead occurs, select Fuji apple fresh, non agricultural chemical residuum for use, clean with clear water, the ovum card is cut into the fritter of being with ovum, the agar block with 1% is attached to the hollow place of apple calyx with small pieces, and big or small by fruit shape, every fruit can connect 8～10 on ovum.Then with apple put into insect box (40cm * 17cm * 17cm) place climatic cabinate to raise, treat that it takes off fruit automatically after, collect mature larva, be put in water content and be in 10% the wet sand, treat its emergence.
Experiment condition:
In climatic cabinate, carry out, climatic cabinate is provided with the same.The trans-anethole concentration of treatment is respectively 20,50,100,500,1000 times of liquid, and solvent is 98% ethanolic solution, is contrast solution with 98% ethanolic solution simultaneously.Different worm attitude processing methods is different, after processing finishes, peach fruit borer is transferred to continuation raising in the climatic cabinate.
1, trans-anethole is to stifling, the action of contace poison of adult
Triangular flask method (improvement) is adopted in this test, with the about 3-5 of the trans-anethole solution second that burr filter paper is put into variable concentrations, dries indoor afterwards, puts into Cans; Each Cans inserts a pair of adult, and bottleneck is shut with preservative film, pricks a little hole on the film; Experiment condition is handled for 10 times with raising term harmonization, each concentration, and each handles repetition 3 times; Contrast is 98% alcoholic solution, observes every day, writes down the once egg laying amount of female worm, and is dead until female adult worm; Change burr filter paper during this time every day, the fate of record male and female adult survival is added up female worm generation egg laying amount.As aforementioned, dissect all female moths at microscopically after the adult death, whether confirm mating according to the shape of its copulatory pouch, statistics adult mating rate.The adult that the test of tagging adopts the potper spray-on process to sprout wings the same day is put into Cans, and sex ratio 1:1 adds the 5ml medicament in the potper sprayer, the dead borer population of statistics after per half an hour.
Table 1 trans-anethole is to the influence of adult
Table 1 is the influence of trans-anethole to the peach fruit borer adult, this shows, trans-anethole has significant insecticidal effect to the peach fruit borer adult; In the experimental concentration scope, utilize the gained killing rate of fumigating, tag all to present positive correlation, promptly along with concentration raises with concentration; The adult lethality is high more, all and between the contrast has utmost point significant difference, between the fumigant insect killing rate scope 38.33%～90.00%; For the stifling mode of adult, 10 times of liquid, 20 times of liquid, 50 times of liquid differences are not remarkable; For the adult mode of tagging, 20 times of liquid, 50 times of liquid, 100 times of liquid differences are not remarkable; All there is 1% utmost point significant difference in all the other; Tag the killing rate scope between 70.00%～100%, and wherein 10 times of liquid, 20 times of liquid, 50 times of liquid 2h cumulative mortalities reach 100%, 100 times of liquid 2h cumulative mortality and reach 98.33 ± 2.89A, have 1% utmost point significant difference between all the other each concentration.
2, trans-anethole is handled the influence of ovum to its hatching
The ovum that 8:00 will collect the same day morning is concentrated picking, places 1cm
2Filter paper on, 20 of every filter papers, each handles 60 ovum, triplicate.Under the indoor conditions; With the trans-anethole drips of solution of variable concentrations on the scraps of paper; Stagnated 3-5 minute, and filter paper was put in the culture dish (d=9cm) of the agar medium that fills 10g/L, culture dish is put in continues in the climatic cabinate to raise afterwards; Observe to no longer hatching the statistics incubation rate continuously.
Between variable concentrations to ovum hatching, moth fruit and to adult influence adopt the SPSS13.0 statistical software to carry out one-way analysis of variance, and draw regression equation, significance level relatively is P=0.05.The lethal concentration of 50 adopts the computational methods of probit value.
Table 2 trans-anethole is to the influence of ovum
Table 2 is that trans-anethole is handled the influence of ovum to the ovum hatching; Can be known that by table 2 trans-anethole has appreciable impact to the hatching of ovum, the concentration and the ovicidal rate of trans-anethole treatment fluid are proportionate in the scope of contrast, 10 times of liquid～1000 times liquid; Promptly along with the rising of concentration, ovicidal rate raises.As proofread and correct ovicidal rate when contrasting ovicidal rate and being 9.00%, 10 times of liquid concentration and rise to 82.05%, The results of analysis of variance shows that each concentration is handled with contrast and had significant difference, part reaches 1% utmost point significant difference.
3, trans-anethole is handled the influence to the moth fruit
Under the indoor conditions, the 3rd day ovum that reveals blackhead concentrated picking, in the time of the 5th day; Be connected on the clean apple of non agricultural chemical residuum, each apple 5-8 grain ovum, microsyringe inject the trans-anethole solution of variable concentrations in the hollow scraps of paper 1 microlitre of locating of apple calyx; 100 ovum of every processing, 3 repetitions, indoor stagnation 3-5 minute; Treat vaporized alcohol, put into climatic cabinate and continue to raise, the statistics borer fruit rate.
Table 3 trans-anethole is handled the influence of ovum to the moth fruit
Trans-anethole is handled ovum table 3 is seen in the influence of moth fruit.The result shows, different disposal concentration, and the borer fruit rate of peach fruit borer is different, and along with the increase of concentration, borer fruit rate descends.There are utmost point significant difference in 10 times of liquid, 20 times of liquid between handling and contrasting, and low concentration trans-anethole medicament for the influence of the effective ovum of peach fruit borer (effectively ovum adult after fertilization is laid eggs) moth fruit not quite.
4, trans-anethole is to the follow-up unearthed influence of mature larva
Infusion process is adopted in test.20 larvas of every processing, triplicate, contrast is 98% alcohol, the mature larva that will collect the same day is put in the different trans-anethole solution of concentration, stagnates for 5 seconds and takes out.Afterwards mature larva is put in the sandy soil of water content 10%, continues to cultivate, add up its eclosion rate.
Table 4 trans-anethole is to the influence of mature larva
Adopt infusion process that mature larva is made an experiment; The Showed Very Brisk phenomenon appears in the examination worm between soak period; Take out the examination worm after 5 seconds and have seemingly-dead phenomenon of short time; It is as shown in table 4 that the examination worm is put into the wet sand statistics rate result of cocooing: except that 1000 times of liquid, all there is significant difference in other concentration with contrast, 10 times of liquid, 20 times of liquid and the utmost point significant difference that contrasts existence 1%.There is not difference between 10 times of liquid～100 times liquid of each concentration of treatment.Between 90.00%～71.67%, explain that trans-anethole is little to the mature larva influence by the borer fruit rate of showing to find out peach fruit borer.When utilizing trans-anethole control peach fruit borer, the mature larva worm attitude stage will not considered as emphasis.
5, the variable concentrations trans-anethole is handled the influence that the non-selective oviposition deterrence of adult is kept away
Lay eggs deterrent effect test method and fumigating method is basic identical, distinguishes to bottleneck uses gauze and shuts, and same concentrations is put in the same climatic cabinate.
The influence that table 5 trans-anethole is kept away the adult oviposition deterrence
Can know by table 5; Trans-anethole is remarkable to the peach fruit borer deterrent effect of laying eggs, and medicament is smoked dead with peach fruit borer during 10 times of liquid, and the oviposition deterrence rate of keeping away reaches 94.13% during 20 times of liquid; And have significant difference between other concentration, the oviposition deterrence rate of keeping away is merely 5.16% during 1000 times of liquid; The mating rate statistical result showed, the solution of high concentration is bigger to peach fruit borer influence, is 36.67% during 20 times of liquid, and 1000 times of liquid when handling mating rate reach 80%, and difference is not obvious between the contrast; And the result that influences in adult life-span is shown: the chemicals treatment of variable concentrations has all shortened the life-span of adult, and male, female worm shortens to 0d by 6.25d, 6.31d, and except that 1000 times of liquid, all there is significant difference in other processing with contrast.
Claims (5)
1. peach fruit borer prevents and treats method, it is characterized in that,
1. trans-anethole and alcohol (volumetric concentration 98%) were formed the liquid of tagging in 1: 100 by volume, be used for 6,7, the utilization means desinsection of tagging before August, fruit was separated bag;
2. trans-anethole and alcohol (volumetric concentration 98%) were formed fumigation liquid in 1: 50 by volume, be used for separating the bag back, utilizing stifling means desinsection before the tree down at fruit.
2. peach fruit borer according to claim 1 prevents and treats method, it is characterized in that, said stifling means are the uncovered bottle of 10cm for said fumigation liquid being placed bore, hangs four in every peripheral evenly cloth of tree.
3. peach fruit borer according to claim 1 prevents and treats method, it is characterized in that, tagging in 1. is arranged in 6,7, August fruit separate before the bag and to carry out once in every month.
4. peach fruit borer liquid of tagging is characterized in that, is made up of in 1: 100 by volume trans-anethole and alcohol (volumetric concentration 98%).
5. a peach fruit borer fumigation liquid is characterized in that, is made up of in 1: 50 by volume trans-anethole and alcohol (volumetric concentration 98%).
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583297A (en) * | 2013-10-18 | 2014-02-19 | 固镇县万佳生态养殖场 | Method for preventing and controlling peach fruit borers of winter jujube |
| CN104839151A (en) * | 2015-04-01 | 2015-08-19 | 金华市农业科学研究院 | Application of Cis-anethol as drug to prevent and treat bee mites |
-
2012
- 2012-05-26 CN CN2012101669606A patent/CN102657164A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 张海燕等: "植物精油防治储粮害虫的研究进展", 《粮食储藏》 * |
| 李晓颖等: "7种非寄主植物挥发物对桃小食心虫繁殖与发育的影响", 《河北农业大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103583297A (en) * | 2013-10-18 | 2014-02-19 | 固镇县万佳生态养殖场 | Method for preventing and controlling peach fruit borers of winter jujube |
| CN104839151A (en) * | 2015-04-01 | 2015-08-19 | 金华市农业科学研究院 | Application of Cis-anethol as drug to prevent and treat bee mites |
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Application publication date: 20120912 |