KR102093394B1 - Composition for controlling mushroom mite and method for controlling mushroom mite - Google Patents

Abstract

The present application relates to a composition for controlling mushroom mite and a method for controlling mushroom mite using the same.

Description

Mushroom mite control composition and mushroom mite control method {COMPOSITION FOR CONTROLLING MUSHROOM MITE AND METHOD FOR CONTROLLING MUSHROOM MITE}

The present application relates to a composition for controlling mushroom mite and a method for controlling mushroom mite using the same.

Mushroom flies, mites, and nematodes are typical pests of mushrooms. Among them, mushroom flies are a pest that greatly damages mycelium, fruiting body tissues, and the medium on which mushrooms grow.

Specifically, mushroom flies usually live by cultivating on forest stalks, compost piles, and decayed timbers in the forest, and when cultivation of mushrooms begins, adult insects are attracted to the peculiar smell of the hyphae and invade into the cultivation fungus Spawns on. Hatched larvae grow while eating mushroom mycelia and fruiting bodies, multiply while repeating the process of becoming pupae and adults, and damage mushrooms (FIGS. 1 and 2).

Mushroom flies usually occur in the late period during the spring cultivation period, but there was no significant damage in the early stages of cultivation, but now, as the area of cultivation increases and the grouping of cultivators grows, the occurrence of mushroom flies transcends regional and temporal aspects. This is happening (Fig. 3). Mushroom fly imago is a major cause of reduced yields and poor quality due to secondary damages because it moves around the mushroom growing fungus and contaminates the fungus with pathogenic bacteria or fungi and mediates mushroom mites and nematodes.

Among the damages to mushrooms, larvae are caused by feeding and cutting hyphae, resulting in a decrease in yield, drilling and eroding fruiting bodies, and contaminating to reduce the value of mushrooms. In addition, adult insects do not directly attack mushrooms, but they cause indirect damage by propagating through various pathogens, nematodes, mites, and fungi that damage mushrooms (FIGS. 4 to 7).

In addition, since the mushroom mite is cut by eating hyphae, when the incidence density is high, it is impossible to harvest the mushrooms at all, and harvesting becomes impossible, which eventually leads to lung disease. Growers that have had mushroom mites even once have re-occurrences if they have not been sterilized, so there is a problem that they must be sterilized before lungs even at a cost.

As an example of a mechanical method for preventing the infestation of adult mushroom flies, a pest control device for mushroom cultivation is disclosed in Korean Utility Model Publication No. 20-2017-0002324, and in addition, a small screen with a small scale is installed in the entrance and ventilation windows. Therefore, a method of suppressing adult infestation in the cultivator can be used. Alternatively, diflubenzuron (Mushroom, Pleurotus), teflubenzuron (mushroom), cyromazine, which is a drug registered for the control of mushroom fly, has an insecticidal effect (IGR: Insect growth regulator) by inhibiting chitin biosynthesis of the body epidermal tissue and suppressing metamorphosis or exfoliation. It is possible to use (slim), but in the case of mushroom mushrooms, it is common to treat even the registered medicines after inoculation, before and after inoculation, and before the occurrence of pine needles. In addition, there are only three types of registered drugs for insecticidal fungi, so even if they are applied alternately, the drug resistance is suspected because they have been used for a long time in cultivated farms.

The use and misuse of organic synthetic pesticides for agricultural pest control measures that are indispensable in crop production can lead to environmental pollution and ecological disturbances such as emergence of resistant pests, residual soil and food, toxicity to livestock, and reduced density of natural enemies and useful insects. There is a problem that it can cause side effects.

Therefore, in the present application, it was intended to provide an eco-friendly natural medicine with excellent effect of killing mushroom mite using tea tree oil, low impact on natural mite and mushroom growth, and low toxicity.

However, the problems to be solved by the present application are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The first aspect of the present application may provide a composition for controlling mushroom mite comprising a tea tree ( Melaleuca alternifolia ) oil.

A second aspect of the present application may provide a method for controlling mushroom mite, comprising applying a distilled tea tree oil to a mushroom mite, a habitat for a mushroom mite, or a predicted habitat for a mushroom mite.

A third aspect of the present application may provide a method for preparing a composition for controlling mushroom mites, comprising diluting tea tree oil in a concentration of 100 to 5000 times in a solvent.

The above-described problem solving means are merely exemplary and should not be construed as limiting the present invention. In addition to the exemplary embodiments described above, there may be additional embodiments and embodiments described in the drawings and detailed description of the invention.

Mushroom mite control method using the composition for controlling mushroom mite of the present invention is an eco-friendly control method capable of increasing the control efficiency of mushroom mite in the mushroom medium, thereby preventing damage due to mushroom mite and improving mushroom productivity to improve farm household income It can contribute to the increase and vitalization of the mushroom industry.

1 is a photographic image of a mushroom that was damaged by the larvae of a mushroom fly.
Figure 2 is a photographic image of a mushroom fly larvae attacking a mushroom.
3 is a photographic image showing a mushroom fly adult.
4 is a photographic image showing a mushroom mite riding on a mushroom fly.
5 is a photographic image showing various types of mushroom mite.
6 is a photographic image of a mushroom that was damaged by a mushroom mite.
7 is a photographic image of a mushroom fungus medium damaged by a mushroom mite.
Figure 8 is a graph showing the measurement of the lethal effect of mushroom mite and Skimitus mite using 500-fold and 1000-fold dilutions of tea tree oil according to an embodiment of the present application.
9 is a photographic image of a process for treating a 1000-fold dilution of tea tree oil to a fungus grower according to an embodiment of the present application.
FIG. 10 is a photographic image of a fungus grower one week after treating a dilution of 1000 times tea tree oil according to an embodiment of the present application.
11 is a photographic image showing a fungus grower treated with a 1000-fold dilution of tea tree oil according to an embodiment of the present application compared to an untreated group.
12 is a graph showing the measurement of the control effect of mushroom mite using a dilution of 1000 times tea tree oil according to an embodiment of the present application.

Hereinafter, embodiments of the present application will be described in detail with reference to the accompanying drawings so that those skilled in the art to which the present application pertains may easily practice. However, the present application may be implemented in various different forms and is not limited to the embodiments described herein. In addition, in order to clearly describe the present application in the drawings, parts irrelevant to the description are omitted.

Throughout the present specification, when a part “includes” a certain component, it means that the component may further include other components, not to exclude other components, unless specifically stated to the contrary.

The terms "about", "substantially", and the like, as used throughout this specification, are used in or at a value close to that value when manufacturing and material tolerances specific to the stated meaning are given, and are understood herein. To aid, accurate or absolute figures are used to prevent unconscionable abusers from unduly using the disclosed disclosure. The term "~ (to) step" or "step of ~" as used throughout this specification does not mean "step for".

Throughout the present specification, the term “combination of these” included in the expression of the marki form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the marki form, the component. It means to include one or more selected from the group consisting of.

Throughout this specification, the description of “A and / or B” means “A, B, or A and B”.

Hereinafter, a composition for controlling mushroom mite, a method for controlling mushroom mite and a method for preparing a composition for controlling mushroom mite will be described in detail with reference to embodiments and examples and drawings. However, the present application is not limited to these embodiments and examples and drawings.

The first aspect of the present application may provide a composition for controlling mushroom mite comprising a tea tree ( Melaleuca alternifolia ) oil.

For example, the tea tree oil may be vegetable essential oil, directly manufactured, or a commercially available product.

For example, the tea tree oil may be extracted using any extraction method for extracting an active ingredient from plants known in the art, but is not limited thereto, water vapor distillation method using water and / or organic solvent, cold It may be extracted by one or more methods selected from the group consisting of a immersion method, a warm immersion method, a distillation method, a hot water extraction method, a leaching method, a compression method, and a supercritical gas extraction method. The organic solvent may be one or more selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, and alcohol, and the alcohol may be a linear or branched alcohol having 1 to 5 carbon atoms, for example, ethanol, methanol, propanol Or it may include butanol, but may not be limited thereto.

For example, the mushroom mite control composition may include the tea tree oil alone, or may include the tea tree oil as a diluted solvent, or may further include other carriers. The content of the tea tree oil may be appropriately adjusted according to the method of use and formulation.

For example, the carrier may be a substance included in a conventional control agent, such as an excipient or a diluent, and examples thereof include saline, buffered saline, dextrose, isoparaffin, water, glycerol and ethanol, but may not be limited thereto. .

The formulation of the mushroom mite control composition can be appropriately adjusted according to the method of use and use, such as liquids, suspensions, granules, warning agents, fragrances, powders, syrups, aerosols, extracts, ointments, fluid extracts, It may be an emulsion, premise, acupuncture, tablet, injection, alcohol, capsule, cream or pill, but may not be limited thereto.

For example, the composition for controlling mushroom mite may be used for controlling mushroom mite, and may be used as a cleaning agent, a pharmaceutical agent, a food additive, and a fragrance, wherein the composition for control may be included in an amount of about 0.01 to 50% by weight. It may not be limited.

According to the exemplary embodiment of the present application, the composition for controlling mushroom mite may be for controlling mushroom mite, but may not be limited thereto.

According to one embodiment of the present application, the mushroom mite control composition may be one that does not exhibit a control effect of Skimitus mite ( Stratiolaelaps scimitus ), but may not be limited thereto. Skimitus mite is a predator of mushroom mite, and the composition for controlling mushroom mite of the present invention exhibits a high control effect on mushroom mite, however, Smite mite mite shows little or no control effect, thus further enhancing the mushroom mite control effect. Can be maximized.

According to one embodiment of the present application, the mushroom mite control composition may be one in which the tea tree oil is diluted about 100 times to about 5000 times in a solvent, but may not be limited thereto.

For example, the composition for controlling the mushroom mite is about 100 times to about 3000 times, about 100 times to about 2000 times, about 100 times to about 1000 times, about 100 times to about 800 times the tea tree oil is in the solvent, About 100 times to about 500 times, about 100 times to about 300 times, about 300 times to about 5000 times, about 500 times to about 5000 times, about 800 times to about 5000 times, about 1000 times to about 5000 times, about 2000 About 500 times to about 2000 times, about 500 times to about 1000 times, about 1000 times to about 2000 times, about 300 times to about 1500 times, about 800 times to about 1200 times, or about 1000 times It may be diluted, but may not be limited thereto.

According to one embodiment of the present application, the solvent may be selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, alcohol, saline, dextrose, isoparaffin, water, glycerol, and combinations thereof, but is not limited thereto. It may not. For example, the alcohol may be a straight chain or branched alcohol having 1 to 5 carbon atoms, and may include, for example, ethanol, methanol, propanol or butanol, but may not be limited thereto.

According to one embodiment of the present application, the mushroom mite control composition may be for use in direct spraying, medium mixing, mushroom inoculation inoculum mixing, prototyping inoculation site insertion, soil mixing, soil irrigation or indirect spray treatment, but is not limited thereto. It may not be.

Specifically, the composition for controlling the mushroom mite is directly sprayed, a method of mixing with a medium, a method of mixing with a mushroom inoculum, and a method of inoculating the original bacteria (usually by drilling a hole in an oak tree and inoculating the mushroom original bacteria) After a certain period of time, the mushrooms will grow. This is how to insert the proper formulation (granulation, tablets, etc.) into the site, how to mix or irrigate into the soil of mushroom growers, and to absorb or spray the appropriate carrier such as non-woven fabric / paper. It can be applied for the control of mushroom mite by placing it around a planter's entrance or a vent.

According to the exemplary embodiment of the present application, the tea tree oil may be tea tree essential oil, but may not be limited thereto.

A second aspect of the present application may provide a method for controlling mushroom mite, comprising applying a distilled tea tree oil to a mushroom mite, a habitat for a mushroom mite, or a predicted habitat for a mushroom mite.

Content described in connection with the first aspect of the present application may apply equally to the second aspect of the present application unless otherwise specified. For example, the tea tree oil diluent may be a composition for controlling mushroom mite according to the first aspect of the present application, but may not be limited thereto.

Mushroom mite control method of the present invention is to control by applying so that the tea tree oil dilution can directly contact the mushroom mite, a method of spraying or applying directly or indirectly to a crop may be used. The spray amount or application amount can be easily determined by a person skilled in the art according to the number of mushroom mites, but the spray amount is 0.05 µl / cm ³ or more based on a unit space, and the application amount is 0.04 µl / cm based on a unit area. ² or more, or 0.064 μl / cm ² or more, or 0.096 μl / cm ² or more, or 0.2 μl / cm ^{2 or} more.

According to one embodiment of the present application, the tea tree oil diluent may be one in which tea tree oil is diluted about 100 times to about 5000 times in a solvent, but may not be limited thereto. For example, the tea tree oil is about 100 times to about 3000 times, about 100 times to about 2000 times, about 100 times to about 1000 times, about 100 times to about 800 times, about 100 times to about 500 times to the solvent , About 100 times to about 300 times, about 300 times to about 5000 times, about 500 times to about 5000 times, about 800 times to about 5000 times, about 1000 times to about 5000 times, about 2000 times to about 5000 times, about 500 times to about 2000 times, about 500 times to about 1000 times, about 1000 times to about 2000 times, about 300 times to about 1500 times, about 800 times to about 1200 times, or about 1000 times diluted, but may be It may not be limited.

According to one embodiment of the present application, the solvent may be selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, alcohol, saline, dextrose, isoparaffin, water, glycerol, and combinations thereof, but is not limited thereto. It may not.

According to one embodiment of the present application, the tea tree oil diluent may be applied by a direct or indirect spray or application method, but may not be limited thereto.

A third aspect of the present application may provide a method for preparing a composition for controlling mushroom mites, comprising diluting tea tree oil in a concentration of 100 to 5000 times in a solvent.

Content described in relation to the first and second aspects of the present application may apply equally to the third aspect of the present application unless otherwise specified.

For example, the tea tree oil is about 100 times to about 3000 times, about 100 times to about 2000 times, about 100 times to about 1000 times, about 100 times to about 800 times, about 100 times to about 500 times to the solvent , About 100 times to about 300 times, about 300 times to about 5000 times, about 500 times to about 5000 times, about 800 times to about 5000 times, about 1000 times to about 5000 times, about 2000 times to about 5000 times, about 500 times to about 2000 times, about 500 times to about 1000 times, about 1000 times to about 2000 times, about 300 times to about 1500 times, about 800 times to about 1200 times, or about 1000 times diluted, but may be It may not be limited.

According to one embodiment of the present application, the solvent may be selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, alcohol, saline, dextrose, isoparaffin, water, glycerol, and combinations thereof, but is not limited thereto. It may not.

The present invention will be described in more detail through the following examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present application.

[Example]

2. Mushroom mite killing effect test
After transferring 10 individual mite mushrooms to a 60 × 15 mm petri dish with 1 filter paper on the floor, 500 or 1000 times dilution (about 1 ml) of tea tree oil was applied (10 repetitions).

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Since the oil component of tea tree oil is not mixed with water in the tea tree oil solution used in the embodiment of the present invention, a solution in which ethanol and the like are mixed with tea tree oil (at a weight ratio, 30 to 90% of tea tree oil + ethanol 1 ~ 10% + Tween 20 1 ~ 10% + DMSO 1 ~ 10%) and diluted to 500 ~ 1000 times, and then used in the experiment.
As a result of spraying 500 and 1000 fold dilutions of tea tree oil, mushroom mites were killed immediately after application when using 500 and 1000 fold dilutions (FIG. 8).

3. Skimitus mite Salvi  Effect test

In the same manner as in Example 2, as a result of spraying 500 and 1000-fold dilutions of tea tree oil with excellent killing power of mushroom mite on the Skimitus mite, in the case of 500-fold dilution, Skimitus mite was 100% lethal, but when using 1000-fold dilution A mortality rate of 3.3% was shown (Fig. 8).

4. Mushroom spray test

Mushroom mite was grown at a high density, and a 30 × 30 cm zone was established by a fungus sawdust grower (Buyeo Seokseong-myeon), sprayed with 1000-fold dilution of tea tree oil (2,000 ml) three times every three days, followed by mushroom mite killing and mushroom growth Was observed. 9 is a photograph showing a process of treating a dilution of 1000 times tea tree oil, FIG. 10 is a photograph of a fungus grower one week after the treatment of a dilution of 1000 times tea tree oil, and FIG. This is a photograph showing comparison of treatment groups. The number of mushroom mite individuals (medicine and adult) of the treated and untreated groups of 1000 times diluted tea tree oil was measured and shown in the graph of FIG. 12.

As a result of the experiment, it was confirmed that spraying the dilution of tea tree oil did not affect the growth of mushrooms, but the mushroom mite density was significantly lowered. In addition, it was expected that the dilution ratio of tea tree oil used in this experiment could be changed according to the scale or environmental conditions of mushroom growers.

As a result of the experiment, a dilution of 1000 times tea tree oil showed excellent insecticidal and killing power to mushroom mites without affecting the growth of mushrooms, while low lethal doses to natural mites, Skimitus mites, could replace organic synthetic pesticides. It is expected to be suitable as an environmentally friendly natural medicine.

The above description of the present application is for illustrative purposes, and those skilled in the art to which the present application pertains will understand that it is possible to easily modify to other specific forms without changing the technical spirit or essential features of the present application. Therefore, the embodiments described above are illustrative in all respects, and it should be understood that they are not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present application is indicated by the claims below, rather than the detailed description, and it should be interpreted that all changes or modifications derived from the meaning and scope of the claims and equivalent concepts thereof are included in the scope of the present application.

Claims (14)

Mushroom mite control, which contains a tea tree ( Melaleuca alternifolia ) oil diluted 1000- to 2000-fold in a solvent, to control mushroom mites arising from mushroom mushrooms, and not showing acaricidal effect against Stratiolaelaps scimitus Dragon composition.
delete delete delete According to claim 1,
The solvent is selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, alcohol, saline, dextrose, isoparaffin, water, glycerol, and combinations thereof, the mushroom mite control composition.
According to claim 1,
Mushroom mite control composition for use in direct spraying, medium mixing, inoculation of fungi progeny inoculation, insertion of protozoal inoculation sites, soil mixing, soil irrigation or indirect spray treatment.
According to claim 1,
A composition for controlling mushroom mite, wherein the tea tree oil is tea tree essential oil.
A method for controlling mushroom mite, comprising applying a composition of claim 1 comprising a dilution of 1000 to 2000 times tea tree oil to a mushroom mite, a mushroom mite or an expected mushroom cultivation of mushroom mite.
delete delete The method of claim 8,
The tea tree oil dilution is a solvent selected from the group consisting of hexane, chloroform, acetone, ethyl acetate, alcohol, saline, dextrose, isoparaffin, water, glycerol, and combinations thereof.
The method of claim 8,
Mushroom mite control method, wherein the dilution of tea tree oil is applied by a spraying or application method.
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