KR20130098765A - Plant-derived insecticidal composition against mushroom fly containing plant-derived oil - Google Patents

Abstract

PURPOSE: A mushroom fly insecticide composition containing plant essential oil is provided to enhance insecticidal effect with the convenience and persistence of treatment. CONSTITUTION: A mushroom fly insecticide composition contains cinnamon essential oil or trans-cinnamic aldehyde as an active ingredient. The cinnamon essential oil is prepared from a Lauceae plant. The cinnamon essential oil is selected among Lauraceae Cinnamomum cassia Blume essential oil and Lauraceae Cinnamomum zeylanicum Nees essential oil. The mushroom fly is selected among Lycoriella mali Fitch, Phorodonta flavipes Meigen, and Bradysia agrestis Sasakawa. [Reference numerals] (AA) Non-treatment; (BB) Sample treatment

Description

Mushroom fly insecticide composition containing plant essential oils {PLANT-DERIVED INSECTICIDAL COMPOSITION AGAINST MUSHROOM FLY CONTAINING PLANT-DERIVED OIL}

The present invention is a mushroom fly control composition comprising cinnamon essential oil or its active compound, trans-cinnamic aldehyde as an active ingredient, mushroom fly control comprising the cinnamon essential oil or its active compound, trans-cinnamic aldehyde in the form of microcapsules Microcapsules composition, and relates to a method for controlling mushroom flies, characterized in that it comprises the step of applying the composition to the crop.

Mushroom flies are classified as Blackwing Mushroom Fly (Sciaridae), Mycetophilidae, Cecidomyiidae and Phoridae in Diptera. The Blackwing Mushroom Fly, also known as Sciarid, is generally dark brown to black with a narrow neck, long tactile and long wings. The larva has black cuticles in the head, and the complete transformation of eggs, larvae, pupa and adult Throughout, one female lays more than 150 eggs, and the first generation is about 21 days at 18 ° C. The mushroom fly family, also known as mycetophil, is the largest adult flies with a body length of 6-7 mm. Its wings and legs are similar to mosquitoes, and its body color is light brown or blackish brown.

Larvae of mushroom flies are about 15-20 mm long. Hominids, also known as Cecids, are dwarfed to about 1 mm in size, have yellow spots on their bodies, larvae are yellow or white and completely metamorphic to about 2 mm, and grow very quickly in juveniles if the environment is good. The first generation of larvae is six days, with one larva producing 6 to 8 larvae. The flea family is called Phorid and is smaller and more active than the black-winged mushroom family. The larva is about 4 mm long and there is no black cuticle on the head. In addition, it is completely metamorphic, and one female spawns about 50 eggs, and the first generation period is about 38 days at 18 ° C and 23 days at 24 ° C.

The most damaging mushrooms in domestic mushroom cultivation are Blackwing Mushroom Fly (Sciaridae), Lycoriella mali Fitch and Blackwing Mushroom Fly ( Phorodonta). flavipes Meigen and small root flies ( Bradysia) agrestis Sasakawa) is divided into three species.

Increasing consumer interest in the value of mushrooms has made them a major source of income for farmers. A comparison of the typical mushroom production yields Lentinus edodes ) has the highest annual yield of mushrooms, followed by Agaricus bisporus . Third, Pleurotus ostreatus ) has been reported to have a high yield.

Mushroom cultivation methods are divided into log cultivation and fungus cultivation. Wood cultivation is one of the mushroom cultivation methods produced from wood such as shiitake mushrooms, mushrooms, hazelnuts, and hazelnuts, which are cut to a suitable length, or cultivated mushrooms by planting seedlings and managing sunshine and ventilation. This method has the advantage of being highly valued by obtaining mushrooms in a more natural state, but recently it has been changing to cultivation as a result of lack of logs or management difficulties. Bacterial cultivation is a method of mixing rice bran or bran with sawdust, adding water, filling it with a plastic bottle, bag or wooden box, making it a medium, sterilizing it, and inoculating the seedlings to cultivate mushrooms. It is becoming.

As the number of farmers grows every year, the production volume and market size increase, and the pests generated by mushrooms are becoming a problem. In particular, mushroom flies are generated regardless of the type and cultivation method of the mushrooms are damaging the mushrooms.

Black-winged mushroom flies and nocturniaceae adults appear in the spring when shiitake logs are inoculated, and it is reported that they spawn mainly in inoculation sites of alleys or in areas with weak germs. In addition, many of these species have been reported to be found in the summer when the larval activity is active by spawning steadily until early summer. The black-winged mushroom fly family occurs from spring to late autumn, and the breeding cycle shows the highest number of adult insects collected in summer. Their path of damage was found to be invasive after spawning, spawning and spawning larvae ate mushroom mycelia and prevented mycelia from growing.

In the case of mycologically-cultivated farmhouses, damage to black-winged mushroom flies and long-tailed mushroom flies has been reported, and larvae are known to occur in a large amount of difficult ventilation in winter because they maintain high humidity and cool conditions throughout the year. The damage caused by black-winged mushroom flies is more pronounced than in normal alley cultivation, and 99% of the collected species have been identified as long-bearded mushroom flies. It also feeds on compost to dissolve the medium and prevent mushroom mycelia from surviving. The most common damages in log cultivation or mycelial cultivation are caused by feeding on hyphae, and sometimes the secondary damage is caused by freshly wrinkling of mature mushrooms. It is known that it has not been removed by processing.

Long-bearded mushroom flies are spawning seasons between March and April, and have been reported to intensify damage from larvae from June to July. House cultivars, in particular, were found to have found more long-tailed mushroom flies in their closed winter months. In addition, long-bearded mushroom flies occur year-round, and the number of adult insects is reported to be high between June and August.

In Korea, the major pests that damage mushroom cultivars have been reported, which belong to the black-winged mushroom, Humpfly, and flea, and the long-winged mushroom, which belongs to the black-winged mushroom, is reported as the most damaging pest. have. In particular, long-bearing mushroom flies, which are known to occur mainly on oyster mushroom medium, have been reported to cause mushroom damage by killing mycelia and fruiting bodies and adding or contaminating the medium, resulting in 17% of mushroom production. Has been reported.

In Japan, there was a similar tendency in Japan, but mushroom flies belonging to the black-winged mushroom family have been reported, such as long-bearded mushroom flies, especially in the case of long-bearded mushroom flies. It has been reported to damage mushrooms and shiitake mushrooms.

Currently, research on mushroom pests in Korea has been reported on the control of mushroom flies, on the development and ecology of mushroom pests, and on the method of classifying mushroom pests in mushroom media. There is no research on the timely investigation of the development of mushroom flies. In addition, research to effectively control this situation is insignificant.

In addition, research on the selection of control drugs for long-tailed mushroom flies, the predominant species among mushroom flies, reported that organophosphorus diazinone, chloropyriphos, IGR-based diflubenzuron, and metoprene were effective in controlling them. The control effect of Pleurotus eryngii flies according to the hydrous treatment was reported. Currently, diflubenzuron hydration, triguard (cyromazine) hydration, and nomalt (teflubenzuron) hydrating agent are registered as control agents for oyster mushrooms, but they occur during shiitake sawdust or shiitake wood cultivation. Research on the control of mushroom flies is still inadequate (Sang-gil Lee, Selection of Agents for Controlling Mushroom Flies by Shiitake Mushroom Cultivator, Monthly Forestry Information, No. 123. 27-32, 2009).

Cyano lead of the zero-friendly control of the mushroom flies contained in the mold opening (Schizonepeta tenuifolia) refinery Poole Gon (pulegone), menthone (menthone) and limonene (limonene) (Lycoriella ingenua ) has been reported to the academia by domestic research teams (Il-Kwon Park, Lee-Sum Kim, In-Ho Choi, Yeon-Suk Lee, and Sang-Chul Shin, J.Eco.Entomol) , 99 (5), 2006, 1717-1721), and also reported that anise and garlic oils have fumigation for the same insect (Il-Kwon Park, Kwang-Sik Choi, Do-Hyung Kim, In Ho Choi, Lee-Sun Kim, Won-Chull Bak, Joon-Weon Choi and Sng-Chul Shin, Pest Manag Sci, 62, 2006, 723-728).

In addition, as an organic chemical control agent, it is known to use deltamethrin, which is developed under the trade names cypermathrin and decis, which are called pyreth in Korea. Cypermethrin is a synthetic insecticide internationally developed under the trade name Lipcode. Although it is effective against a wide range of pests due to digestive poisoning and contact poisoning, it is highly toxic to fish and bees and has been reported to be toxic to humans. It is a pesticide that should be careful. Deltamethrin is a synthetic pyredrin that supplements the disadvantages of Pyredrin, a pesticide international, as a pesticide, and has similar toxicity to cypermethrin. In particular, it is reported to be highly toxic to silkworms, fish and shellfish.

Considering the degree of damage of the pest and the lack of the developed drugs as described above, it can be said that the development of a drug that is not resistant to all these pests and exhibits effective pesticidal activity.

The present inventors broiler lauraceae (Lauraceae) against pests of the mushroom flies, especially black mushroom wing tanks causing severe damage to the mushroom crop pests as mushroom cultivation (Cinnamomum Cinnamon essential oils obtained from the leaves of cassia Blume) and stems or stem bark or stem bark of Lauraceae Cinnamomum zeylanicum Nees were found to have excellent insecticidal activity and to develop a microcapsule composition containing them as an active ingredient. The invention was completed. In addition, by providing a functional net sculpture treated with the composition for controlling mushroom flies in the net, the insecticidal effect in terms of ease and persistence in the technical aspect has been increased.

Thus, one example of the present invention provides a composition for controlling mushroom fly comprising a plant essential oil as an active ingredient. The composition may be in the form of a microcapsule.

Another example provides a mushroom cultivation sculpture treated with the composition for controlling mushroom flies.

Another example provides pest control products (eg, insecticides and / or repellents) comprising the composition for controlling mushroom flies.

In one example of the present invention, there is provided a composition for controlling mushroom flies comprising plant essential oil as an active ingredient. The composition may be in the form of a microcapsule.

The plant essential oil is cinnamon essential oil, more specifically Lauraceae broiler ( Cinnamomum Plant essential oils extracted from cassia Blume, and Cinnamomum lauraceae It may be a plant essential oil extracted from at least one camphor tree selected from the group consisting of plant essential oils extracted from zeylanicum Nees.

In another example, there is provided a mushroom cultivation sculpture treated with the composition for controlling mushroom flies.

Hereinafter, the present invention will be described in more detail.

The composition for controlling mushroom flies is a insecticide and / or repellent composition of natural ingredients, mushroom flies, for example, mushroom flies, for example, mushrooms that are the main pests of mushrooms ( Lycoriella). mali Fitch), Blackwing Mushroom Fly ( Porodonta) flavipes Meigen, small root flies ( Bradysia) agrestis Sasakawa), characterized in that it has insecticidal and / or repelling activity against at least one pest selected from the group consisting of mushroom flies.

As used herein, the 'mushroom cultivation sculpture' is treated with a composition containing the plant essential oil (cinnamon essential oil) as an active ingredient having a pesticidal and / or repelling effect against mushroom pests, such as mushroom fly pests It means all sculptures commonly used for mushroom cultivation characterized in that. The material and size of the 'mushroom cultivation sculpture' is not particularly determined, and means all the sculptures commonly used in mushroom plantations, for example, a light shielding film, a cover, an insect screen (for example, the size of the body is 0.1mm) Or more than 0.5 mm, specifically 0.5-1 mm screen). The 'mushroom cultivation sculpture' is often in the form of a fibrous material, it is also referred to herein as a functional net for mushroom cultivation.

In addition, unless otherwise specified herein, 'microcapsule composition' refers to a composition in which the cinnamon essential oil obtained from the plant is processed to the size of micro units through a conventional encapsulation step. The average particle size of the microcapsules is on the order of 0.1 to 100 micrometers, specifically 0.5 to 10 micrometers. The encapsulation may be by the method illustrated in FIG. 2.

The plant essential oil is called cinnamon essential oil and may be an essential oil obtained from camphor plants, specifically broilers and / or jades. The plant essential oil may be any extraction method for extracting an active ingredient from a plant known in the art, for example, water and / or an organic solvent (linear or branched alcohol having 1 to 4 carbon atoms, or 5 to 10 carbon atoms). Vapor distillation using alkane, such as ethanol or methanol or hexane), cold distillation, hot distillation, distillation, hot water extraction, hot water extraction, reflux extraction, leaching, compression, supercritical gas extraction, steam distillation One or more methods selected from the group may be used, but are not limited thereto. For example, when the reflux extraction method is used, extraction may be performed once to five times, for example, twice at a temperature of about 60 to 10 ° C., for example, about 80 ° C. for 2 hours to 4 hours, such as about 3 hours. In addition, in the case of using the cold acupuncture method, the extractant may be administered at a temperature of 1 to 20 times, for example, about 10 times (volume) of the herbal material at room temperature, and cooled for about 3 days to extract 1 to 5 times, for example, 2 times. Can be. In one embodiment of the present invention was used plant essential oils obtained by steam distillation.

The mushroom fly is a pest that damages the plant cultivation, specifically, may be one or more selected from the group consisting of long beard mushroom fly, black-wing mushroom fly, small root fly. They eat the mycelia of the mushrooms in the larval stage, preventing them from growing, and in severe cases, they belong to the pests that damage the mushrooms at the harvest time and reduce their merchandise. In general, damage caused by larvae is more serious than adults. Among mushroom flies, the damage to long-bearing mushroom flies is the most severe.

Thus, the composition and moldings for controlling mushroom flies of the present invention are characterized by having excellent control (insect and / or repellent) effects against mushroom flies, especially long-bearing mushroom flies. In addition, in view of the long time of occurrence of these pests, an example of the present invention is characterized by improving the sustainability of the effect by formulating a composition for controlling mushroom flies in the form of microcapsules by introducing a technique called microcapsulation. In another example, in consideration of the fact that the frequent watering is made during the mushroom cultivation, the pest control composition is treated by treating the control composition to the mushroom cultivation sculptures such as the point where the pest is introduced or the point attracted by light, shading film, etc. Characterized by providing a sculpture for mushroom cultivation designed to be made at the same time control.

Meanwhile, broilers and jades, which are the source of plant essential oils used in the present invention, are a camphor tree (Lauraceae) plant. In general, the leaves are shifted to shallow leather and have a scent, oval or egg-shaped oval, and 6 to 10 cm in length. 3-6 cm, acute low, basal, wide wedge-shaped or rounded. It has hairs on both sides, wavy serrates on the edges, gray green on the back, but young leaves are reddish, three veins are distinct, and two vesicles (a kind of preemption) are usually located at the branching points of the three rows. Petiole 1.5-2.5cm long, without hairs. Fruits are berry, round, 8mm in diameter, ripen in black from October to November. The flower is a male and female flower, blooms in May, white to yellow, and runs in a conical inflorescence from the axilla of the branch. The flower is small with a diameter of 4.5mm. Hwajeophaek arranged in 2 rows of 3 each, there are 12 stamens and 1 pistil arranged in 4 rows, the inner surgery is not anther. The stem is more than 20m high, the diameter reaches 2m, and the annual branch is yellowish green, glossy, with shell eyes but without hairs. The bark is dark brown and deeply dug vertically. Dong-A's scales are tiled. Roots have one root and side roots, and their distribution is cultivated in China, Vietnam, Taiwan, and Japan. It is an evergreen broad-leaved arboreous tree, and its shape is original.

In Korea, broilers of camphor tree ( Cinnamomum cassia Blume) or the bark or bark of the cognate plant is called cinnamon. The definition of cinnamon is that China is the same as Korea, and Japan is the broiler with Cinnamomum zeylanicum Blume) is a process medicine. Thus, the plant essential oil of the present invention may be referred to as cinnamon essential oil.

The broiler is Cinnamomum broiler from Camphoraceae in Korea and China. The bark of cassia Blume) is commonly referred to as the broiler. The broiler treats cold sores, lower back and knee weakness, lack of nourishment, frequent urination and diarrhea, diarrhea, diarrhea, vomiting, menstrual disorders, menstrual pain, postpartum dysmenorrhea, boils, skin ulcers, and quadriplegia pain. It was reported to write. Pharmacological actions have been reported to have central suppression, sedation, analgesic, antipyretic, increased coronary blood flow, and skin fungal suppression. Appearance is plate-like or plate-shaped rolled inward. The outer surface is grayish brown, and the fine lines and side pores protrude. Pale white spots in several places, inner side red or brown. Other names include statistic, lager, grandfather, mycelium, chicken, maternal, rhesus, cinnamon, relations, herds, and magnetic fields.

In the case of Lauraceae broiler ( Cinnamomum cassia Blume), the plant extraction target region may be one or more sites selected from the group consisting of whole plants, leaves, twigs, and stem bark, and Lauraceae jade system. ( Cinnamomum zeylanicum Nees) may be one or more sites selected from the group consisting of whole plants, stem bark and the like. The stem bark means cinnamon, that is, bark which is somewhat removed from the bark or the bark. When using any part of the leaves, branches, or stem shells of broilers or jades for the collection of plant essential oils, plant essential oils of similar composition, including cinnamic aldehyde, can be obtained.

Production of the plant essential oil of the present invention can be carried out by a conventional plant extraction method, it can be used for extraction by using all or part of the dried or undried plant as it is or crushed. After the extraction, it may be subjected to conventional filtration and / or drying procedures. The extraction method, as described above, may be by any extraction method for extracting the active ingredient from a plant known in the related art, for example, steam distillation, cold immersion, warm immersion, distillation, hot water extraction, hot water extraction, reflux One or more methods selected from the group consisting of an extraction method, a leaching method, a compression method, a supercritical gas extraction method, etc. may be used, but are not limited thereto. In one embodiment of the present invention, the extraction may be by steam distillation using at least one selected from the group consisting of water and an organic solvent as the extraction solvent, the organic solvent is a linear or branched alcohol having 1 to 4 carbon atoms and It may be at least one selected from the group consisting of alkanes having 5 to 10 carbon atoms, and may be at least one selected from the group consisting of ethanol, methanol, and hexane. In addition, the plant essential oil may be obtained by additionally separating and purifying by using various conventional chromatographic methods.

In one embodiment, the plant essential oil is one or more sites selected from the group consisting of whole plants, leaves, twigs, and stem bark of Lauraceae Cinnamomum cassia Blume, and / or Lauraceae japonica ( Cinnamomum One or more sites selected from the group consisting of whole plants, stem bark and the like of zeylanicum Nees) are extracted by steam distillation, followed by layer separation with alkanes having 5 to 7 carbon atoms such as hexane to collect supernatant, ie It may be obtained by taking a component dissolved in an alkane having 5 to 7 carbon atoms, such as hexane.

In addition to the active ingredient, the composition for controlling mushroom flies may be used in combination with conventional insecticides, acaricides and / or fungicides, and may further include excipients or carriers used for these insecticides, bactericides and acaricides. The excipient or carrier may be a conventional material for enhancing ease of use, economy, formulation or insecticidal effect, and examples thereof include flavors, emulsifiers, alcohols and dispersants, but are not limited thereto.

On the other hand, the present inventors confirmed the mushroom fly control effect of trans-cinnamic aldehyde in the essential oil component of cinnamon (Experimental Example 3)

Therefore, in another example of the present invention, the composition for controlling mushroom flies may include a component effective for controlling mushroom flies separated from components of cinnamon essential oil, such as trans-cinnamic aldehyde.

In another example, a mushroom fly control product (eg, insecticide and / or repellent) comprising the above composition for controlling mushroom fly is provided.

The pest control product may be formulated in a suitable form according to the purpose and use, for example, granules, powders, solutions, aerosols, sprays, extracts, pastes, liquid extracts, emulsions, suspensions, It may be a capsule, a liquid hydrating agent, a granulating hydrating agent, a hydrating powder, a powder, a fine powder, an oil, a gelling agent, a smoker, a fumigant, and the like, and an emulsion, a smoker, a fumigant, or an aerosol agent is most preferable. Do.

The content of camphor plant essential oil and / or trans-cinnamic aldehyde as an active ingredient in the pest control product can be appropriately adjusted according to the purpose of use, product type, target pest, target crop, and the like.

A method of attaching a composition for controlling mushroom flies (for example, a composition formulated in a microcapsule form) to a sculpture (fiber) may include dip dyeing, padding, printing, and the like.

In the dip dyeing method, the binder resin (binder), the microcapsule composition and the diluting solvent are mixed well to prepare a mixed solution, and the fibers are immersed therein to bind the microcapsules to the fibers.

For example, the concentration of camphor plant essential oil as an active ingredient 20 to 30% (w / w) of the composition for controlling mushroom flies 1: 0.5 to 1.5 (volume for control composition: volume of the binder resin), such as a binder resin, Mix 1: 1 and dilute 50 to 100-fold based on the composition using one or more selected from the group consisting of water, alcohols (such as linear or branched alcohols having 1 to 4 carbon atoms), excipients, etc. as diluent solvents. You can use one. For example, 1 ml of the binder resin is mixed with 1 ml of a mushroom fly control composition (for example, a composition formulated in a microcapsule form) having a plant essential oil concentration of 20 to 30% (w / w), and then 48 ml to 98 ml of water as a diluting solvent. Can be used. At this time, the content of the active ingredient (plant essential oil) in the pest control product is 0.6% (v / v) for 50-volume dilution assuming that the content of the plant essential oil in the composition for controlling mushroom fly is 30% (w / w) 0.3% (v / v) for 100 volume dilution. Therefore, when the content of plant essential oil in the composition for controlling mushroom flies is 20 to 30% (w / w), the content of plant essential oil in the pest control product is 0.4% (v / v) to 50 volume dilution. 0.6% (v / v), 0.2% (v / v) to 0.4% (v / v) for a 100 volume dilution (see Experimental Example 1 and Table 2).

Therefore, the concentration of plant essential oil as an active ingredient in the pest control product is at least 0.01% (v / v), at least 0.1% (v / v), or at least 0.3% (v / v), for example, 0.01 to 50% (v). / v), specifically, about 0.3 to about 10% (v / v).

In addition, the concentration of trans-cinnamic aldehyde as an active ingredient in the pest control product is 0.01% (v / v) or more, 0.1% (v / v) or more, or 0.2% (v / v) or more, for example, 0.01 to 50 % (v / v), specifically about 0.2 to 10% (v / v).

The pest control product may be formulated with a binder resin that helps to adhere to the mushroom cultivation moldings such as nets in the form of a container containing the composition for controlling mushroom flies, homogeneous to the net by mixing the composition and the binder resin It can be commercialized in a coated form.

The padding method is to sufficiently immerse the fiber fabric in the well mixed 0.1-2% by weight of the microcapsule composition, 0.1-2% by weight of the binder resin, and 96-99.8% of the diluent solvent, and the microcapsule composition and the binder resin penetrate deeply into the fiber fabric. After drying, the drying process (Dry) is carried out. At this time, the drying process is a tenter process and is dried in a continuous process at about 120 to 180 ° C. During the drying process, crosslinking occurs between the mixed microcapsule composition, the binding resin and the fiber fabric, and thus the microcapsule composition has the binding force, and the crosslinking reaction between the microcapsule composition and the fiber fabric is carried out for a long time. It has a pesticidal effect. When the padding method is used, the fabric is immersed in the mixed solution to adjust the pick-up rate to about 50 to 80% .In particular, when the fabric is less water absorbent such as polyester or nylon, the pick-up rate may be increased to 90%. By adjusting the rate, it is possible to control the amount of the insect repellent microcapsules composition to be treated in the fiber fabric. This padding method can be used for various fabrics such as cotton fabric, polyester, nylon, polypropylene, rayon, and the like. The diluent solvent may be at least one selected from the group consisting of water, alcohols (eg, straight or branched alcohols having 1 to 4 carbon atoms), excipients, and the like.

The printing method is a method of printing and coating in a certain shape on the fiber fabric by mixing the printing resin and microcapsules composition for printing, and mixing 20 to 40% by weight of the microcapsule composition with 60 to 80% by weight of the bonding resin and In order to match the viscosity of the size, a small amount of thickener is added, followed by well stirring and mixing. This is printed on a silk printing plate for printing a certain shape engraved and printed after printing and printing.

In an embodiment of the present invention, at least one selected from the group consisting of melamine-based, polyacryl-based, polyurethane-based, polyamide-based, acrylic styrene-based resins, and the like as the water-soluble binder resin used to attach the microcapsule composition. It may be used, in general, a glass transition temperature of -50 to 20 ℃ can be used.

In another example, there is provided a sculpture for mushroom cultivation to which the composition for controlling mushroom fly is applied. The mushroom cultivation sculpture is applied to the mushroom fly control composition of the present invention by a conventional method such as dipping, coating, spraying, may be selected from the group consisting of a light shielding film, cover, insect screen (awning, etc.), the material is synthetic resin , Synthetic fibers, synthetic rubber, and the like. In addition, it may be the surrounding facilities themselves, and may include mushroom culture logs and culture medium.

In another example, there is provided a pest control method comprising applying the mushroom fly control composition to a crop (mushroom).

The composition for controlling mushroom flies can be used by diluting to an appropriate concentration during use.

Dilution concentrations may be appropriately adjusted depending on the crop used and / or the purpose of use. In one example, even when diluted 1 to 100 times by volume based on the mushroom fly control composition of 20 to 30% (w / w) concentration of plant essential oil as an active ingredient shows excellent control effect (Experimental Example 1 and Table 1) . Therefore, the dilution concentration is 0.01% (v / v) to 50% (v / v), specifically 0.2% (v / v) to 30% (v / v) based on the concentration of plant essential oil as an active ingredient. Can be used to

As the dilution solvent, one or more selected from the group consisting of water, alcohols (eg, linear or branched alcohols having 1 to 5 carbon atoms), and conventional excipients may be used. For example, water may be used.

The pest control method is a method of spraying or spraying the diluted or undiluted mushroom fly control composition directly to the crops and / or soil in the facility, applied to or sprayed on the mushroom cultivation sculptures such as nets (insect net), or fumigated It may be applicable. In particular, when using fumigation and fumigant, it is appropriate to use 30 ~ 120 mg / m ² per unit area and 50 ~ 120mg / m ³ per unit space based on the amount of active ingredient. However, since its amount is differently applied depending on the growth conditions and size of the crop, it is not limited to the above range.

In addition, the crop applied to the insecticidal action according to the present invention is not particularly limited, and may be, for example, one or more selected from the group consisting of shiitake mushroom, oyster mushroom, situation mushroom, enoki mushroom, matsutake mushroom, mushroom mushroom, matsutake mushroom and the like.

Mushroom fly control composition according to the present invention exhibits excellent insecticidal activity against mushroom fly pests that are severely damaged in facility plantation or outdoor packaging, and more preferably excellent insecticidal activity against the black-wing mushroom flies and long-bearing mushroom flies Therefore, it can be usefully used as an effective and environmentally friendly pest control means.

The present invention is a microcapsule emulsion composition that maximizes the activity from the extract composition derived from cinnamon plant to the composition consisting of the main containing components or extracts or compounds comprising at least one of them and the damage caused by mushroom cultivation as a sculpture using the same Excellent insecticidal activity against mushroom flies giving. In addition, the composition is environmentally friendly, and can effectively control the mushroom fly can be used to replace the pesticides mainly synthetic synthetic chemicals.

1 is a photograph of mushroom hump flies and long-bearing mushroom flies among the target insect mushroom flies for the invention.
Figure 2 is a diagram illustrating a series of processes for producing a microcapsule formulation with an active ingredient of plant essential oil.
3 is a scanning electron micrograph of the cinnamon-containing microcapsules composition described in Example 3.
FIG. 4 is a photograph confirmed by scanning electron microscopy of a cinnamon-containing microcapsules described in Example 3 on a net .
FIG. 5 is a diagram illustrating an activity test by immersing a simple assay net in a test sample (Example 3) used in Experimental Example 1, comparing the sample treatment and no treatment.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

[ Example  One]

Preparation of Plant Essential Oils

Lauraceae Broiler ( Cinnamomum 3 kg of stem bark of cassia Blume) was purchased and pulverized, and the essential oil components were collected while extracting at 100 ° C. using a steam distillation apparatus. The collected essential oil components were separated using hexane in a steam distillation apparatus, and only the hexane layer, which was a supernatant, was separated and concentrated under reduced pressure at 35 ° C. (EYELA autojack NAJ-160, Japan) to obtain a final oil. Was 4% by weight.

The trans-cinnamic aldehyde standard, an active ingredient (indicator) of cinnamon essential oil, was purchased from Sigma-Aldrich and used for pesticidal activity and stability experiments.

[ Example  2]

Preparation of Plant Essential Oil Formulations

30 g of the essential oil of cinnamon of Example 1, 20 g of an emulsifier (Castor oil, ethoxylated), and 50 g of ethanol (95% (v / v)) were mixed and stirred at about 200 rpm or more for about 20 minutes to prepare an emulsion composition of cinnamon essential oil. It was. The initial dispersibility for the emulsion was 3DHW / 20 ℃ -30 ℃, 20-300 times, and the emulsion stability showed stability for 3DHW / 20 ℃ -30 ℃, 20-300 times / 24hr. Stability for the formulations ranged from -10 ° C to 54 ° C.

[ Example  3]

Preparation of Microcapsule Compositions and Sculptures

(1) Preparation of Microcapsule Composition

For outer wall materials of functional microcapsules, melamine-glutaaldehyde condensates, melamine-formalin condensates, urea-formalin condensates, urea-glutaaldehyde condensates, melamine-urea-formalin condensates, epoxy-urethane condensates Various polymer materials such as urethane condensates, epoxy condensates, chitosan and acrylic polymers can be used. However, gelatin-glutaaldehyde condensates have higher stability and control the release rate of the outer wall of the capsule. It is preferable to use capsules of gelatin-glutaaldehyde condensates as this is possible.

Therefore, in this study, microcapsule composition using gelatin-glutaaldehyde condensate was prepared and used. The manufacturing process is largely homogenization step through dispersion of encapsulation target component, encapsulation step to form a film, and stabilization after condensation. The process was divided into curing steps.

30 g of plant essential oil obtained in Example 1, 10 g of styrene-maleic acid copolymer (Sigma-Aldrich, molecular weight: 220.22), 20 g of glutaraldehyde (glutaraldehyde, Sigma-Aldrich), 5 g of sodium hydroxide, and PVA (Polyvinyl alcohol, CAS NO) 9002-89-5, Sigma-Aldrich) (CH 2 CHOH) n; Molecular weight: ((44.05) n) (≥50,000))) 15 g, and 20 g of gelatin were mixed and put into a reactor, and stirred sufficiently at 1,000 rpm for 1 hour at 60 to 120 ° C.

As described above, the cinnamon plant essential oil, which is an encapsulation target component, is mixed with a mixed solution of microcapsule outer wall materials through a polymerization reaction to a uniformly stirred material to form a film through a condensation process. Encapsulation of the was made. After that, it was confirmed whether the encapsulation was made well in the form of a sphere through an electron microscope (see FIG. 3). When the screening process was completed, the resultant was subjected to coagulation, and the final microcapsule formulation was prepared by stabilizing through aging (Ageing) for a while.

The microcapsule formulation preparation method is illustrated in FIG. 2.

(2) Measurement of physical properties of microcapsule formulation

The physical properties of the microcapsule formulations obtained as described above are measured and shown in Table 1 below.

Item unit Spec. Remarks Exterior Milky liquid Visual identification pH 5.5 to 8.5 Solid content wt.% Min 28 ~ 32% Accurate solids measurement is impossible due to the nature of oil refinery (105 ℃ 3 hours dry recipe detection theory) Oil refinery wt.% 30%
(Min: 25%) Recipe Detection Theory Capsule size ㎛ 3 (± 2) Electron microscopy Viscosity CPS Mix 500 Brookfield. 6 rpm Stability temperature ℃ Mix 170 ℃ Dry open 160 ~ 170 ℃ Varies by stable heat contact Preservation Period year Mix2 years 1 year 6 months or more

In order to observe the shape and particle size of the microcapsules formulation prepared as described above, a field-emission scanning electron microscope (Field-Emission Scanning Electron Microscope, SUPRA 55VP, Carl Zeiss Co. Germany) was used, and the results are shown in FIG. 3. Indicated. As shown in FIG. 3, the average particle size of the microcapsule formulation was about 0.5 to 2.5 micrometers, considering that it was well formed in spherical shape or size.

(3) Preparation of sculptures to which microcapsules are applied

In order to stably apply the prepared microcapsule composition to a sculpture such as cloth, a binder resin was added thereto, and the binder resin was mixed at a volume ratio of 1: 1 with the microcapsule composition. Thereafter, water was used as a diluting solvent, and a 50 volume dilution and a 100 volume dilution were used.

Specifically, 1 ml of the binding resin is mixed with 1 ml of the microcapsule composition having a plant essential oil concentration of 30% (w / w), water is added in an amount of 48 ml to 98 ml, diluted 50 to 100 times by volume, and the concentration of the final active ingredient is Prepared in a 200 ml beaker with 0.6% (v / v) to 0.3% (v / v). A cloth of appropriate size (5 cm × 5 cm, eyeball size of 0.5 mm or more) was prepared in the prepared composition and stirred for 30 minutes so that the composition was evenly attached to the cloth. After drying well in the shade and observed the particle morphology using a field-emission scanning electron microscope (Field-Emission Scanning Electron Microscope, SUPRA 55VP, Carl Zeiss Co. Germany).

The observed results are shown in FIG. 4. The upper figure of FIG. 4 is a photograph with a reference bar of 1 micrometer, and the lower figure is a photograph with a reference bar of 10 micrometers. It can be seen that the composition in the form of microcapsules is stably attached to the treated fabric, and some of the microcapsules are broken by causing friction with the cloth to increase the insecticidal effect, and the following mushroom fly insecticidal test Used for.

[ Example  4]

Preparation of Effective / Indicator Formulations of Cinnamon Essential Oils

3 g of a trans-cinnamic aldehyde standard product, an active ingredient (indicator component) of cinnamon essential oil of Example 1, 2 g of an emulsifier (Castor oil, ethoxylated), and 5 g of ethanol (95% (v / v)) at about 200 rpm or more The mixture was stirred for about 20 minutes to prepare an effective / index component emulsion composition of cinnamon essential oil. The initial dispersibility for the emulsion was 3DHW / 20 ℃ -30 ℃, 20-300 times, and the emulsion stability showed stability for 3DHW / 20 ℃ -30 ℃, 20-300 times / 24hr. Stability for the formulations ranged from -10 ° C to 54 ° C.

[ Experimental Example ]

For use in experiments Long Beard Mushroom Fly  Adult preparation

Lycoriella mali Fitch is a system in which the adult insects collected in Jangheung, Jeonnam, were raised in the laboratory without pesticides, and had a temperature of 20 ± 4 ℃, a relative humidity of 40 to 60%, and no exposure to light. Under the condition of memorization, after breeding, the seedlings were provided with a damaged medium and mushroom seedlings, and the adult molars were used for the activity test after 2-3 days of breeding.

[ Experimental Example  One]

Insecticidal activity of emulsion compositions and microcapsule emulsion compositions

 Insecticidal activity against the 30% (w / w) emulsion composition (Example 2) and the 30% (w / w) microcapsule formulation (Example 3) was examined in the following manner:

Take 1 ml of the cinnamon essential oil alone emulsion composition (cinnamon essential oil concentration 30% (w / w)) prepared in Example 2 and diluted 100 times with water, the final processing concentration of cinnamon essential oil in the dilution 0.3% (v / v) Prepared in a 200 ml beaker. Cylindrical cloth (size of eyeball is 0.5 mm) to hold the prepared quince mushroom fly adulter was immersed in a beaker containing 0.3% (v / v) cinnamon emulsion composition prepared in advance for 10 minutes to sufficiently absorb the composition, and then completely dried. A cylindrical cage was made by covering a cylindrical wire mesh with a diameter of 10 cm and a height of 20 cm. Thereafter, 20 adult insects of mushroom flies were sucked into the suction tube and placed in a drug-treated cage to observe the insecticidal activity. The experiment was repeated three times. As a control, 20 adult insects of mushroom flies were observed in a cylindrical cage without any treatment. Observation was carried out at regular time intervals and the results are shown in Table 2 below.

As confirmed in Table 2, more than 78% of the insecticidal activity was observed after 2 hours and 30 minutes.

1 ml of the binder resin is mixed with 1 ml of the microcapsule formulation containing cinnamon essential oil prepared in Example 3 (cinnamon essential oil concentration 30% (w / w)), diluted 10 vol. To 100 vol. The final processing concentration of the essential oil was 0.3% (v / v) to 3% (v / v) was prepared in a 200 ml beaker. Prepare a cylindrical mesh (cloth) to contain the prepared quintet mushroom fly as the number of concentration and soak for 10 minutes in a beaker containing the 0.3% (v / v) to 3% (v / v) microcapsule formulation prepared before After sufficiently absorbing the formulation, it was completely dried, and a cylindrical cage was made by covering a cylindrical wire mesh having a diameter of 10 cm and a height of 20 cm. Thereafter, about 20 adult male mushrooms of long-bearing mushrooms were sucked into the suction tube, and the insecticidal activity was observed in the drug-treated cage. The experiment was repeated three times, and as a control, 20 adult mushroom fly adults were observed in a cylindrical cage without any treatment (FIG. 5). Observation was carried out at regular time intervals and the results are shown in Table 3 below.

As shown in Tables 2 to 3, the pesticidal activity of the 30% (w / w) microcapsule formulation is better than that of the 30% (w / w) emulsion composition, and the concentration-dependent concentration increases the concentration to 100%. The time it appeared was shortened. This can be confirmed that the microcapsule formulation retains the active ingredient for a certain time and gradually diverges, considering the volatilization due to the nature of the essential oil component.

[ Experimental Example  2]

Insecticidal Activities of Positive Control and Microcapsule Formulations

As a positive control for the comparison of insecticidal properties, 5% (w / w) cypermethrin (Kyung-Nong) and 2% (w / w) deltamethrin emulsion (Elfam) were obtained. Used. In the previous simple assay, when the two compounds were diluted 10 times by volume, the concentration was lowered to 100, 200, and 400 volume dilutions because they showed 100% insecticidal rate. The obtained results are shown in Table 4.

As shown in Table 4, the deltamethrin 100-volume dilution showed a pesticidal rate of about 90% or more from about 20 minutes, and the ciphermethrin 100-volume dilution showed a 70% pesticidal rate from 30 minutes or more. Both insecticides showed 100% pesticide rate. Compared to the two positive controls, the 1.5% (v / v) microcapsule formulation showed at least 75% pesticide at 20 minutes and 100% at 30 minutes. As a result, the 1.5% (v / v) microcapsules formulation was better compared to the positive control 100-fold dilution. Considering that the positive control group is a synthetic formulation consisting only of the active ingredient compound, it can be seen that the insecticidal effect obtained in the formulation of the present invention as a natural plant essential oil component is very excellent.

Sustainability Test of Insecticidal Activity of Positive Control and Microcapsule Emulsion Compositions

To test the sustainability of the formulations, 100%, 200, and 400, respectively, were treated with 0.9% (v / v) microcapsule formulation and 5% (w / w) cipermethrin and 2% (w / w) deltamethrin emulsion as positive controls. After diluting with the volume, it was processed to the net and proceeded to the test condition after 12 hours (see Experimental Example 1). The results obtained are shown in Table 5 below.

As shown in Table 5, data showing concentration dependent effects were obtained, and it was confirmed that the sustained activity of the microcapsule formulation was superior to that of the positive control group.

[ Experimental Example  3]

Efficacy Assay for Effective / Indicator Ingredient Formulations of Cinnamon Essential Oil

1 ml of cinnamon essential oil prepared in Example 4 (indicator ingredient concentration 30% (w / w)) in 1 ml of water as a diluting solvent diluted 15 times, 30 times, 60 times and 120 times based on volume ratio Thereafter, the insecticidal effect on the long beard mushroom flies was tested in the same manner as in Experimental Example 1 above. The results obtained are shown in Table 6 below.

As can be seen from Table 6, 100% pesticidal activity was seen at 30 to 60 minutes at an active ingredient concentration of 0.5% (v / v).

Sustainability Test of Insecticidal Activity against Effective / Indicator Formulations of Cinnamon Essential Oil

Persistence test was conducted for the active ingredient of the formulation trans-cinnamic aldehyde. Sustainability was assessed by storing the tested formulations on an initial gannet and testing for pesticide maintenance after 12 hours and 24 hours.

As shown in Table 7 and Table 8, 100% pesticidal activity was confirmed at 240 minutes after the measurement at the active ingredient concentration 0.5% (v / v).

Claims (12)

Mushroom fly control composition comprising cinnamon essential oil or trans-cinnamic aldehyde as an active ingredient. According to claim 1, wherein the cinnamon essential oil is a plant essential oil extracted from the camphor tree, mushroom fly control composition. According to claim 2, wherein the cinnamon essential oil is at least one selected from the group consisting of plant essential oils extracted from Lauraceae Cinnamomum cassia Blume and plant essential oils extracted from Lauraceae Cinnamomum zeylanicum Nees. , Mushroom fly control composition. The method according to any one of claims 1 to 3, wherein the mushroom fly is Lycoriella mali Fitch), Blackwing Mushroom Fly ( Porodonta) flavipes Meigen), and small root flies ( Bradysia agrestis Sasakawa) is one or more selected from the group consisting of, mushroom fly control composition. Contains cinnamon essential oil or trans-cinnamic aldehyde as an active ingredient,
Have an average particle size of 0.1 to 100 micrometers
Microcapsules composition for controlling mushroom flies. The microcapsule composition for controlling mushroom flies of claim 5, wherein the cinnamon essential oil is a plant essential oil extracted from the camphor tree. The method of claim 6, wherein the cinnamon essential oil is at least one selected from the group consisting of plant essential oils extracted from Lauraceae Cinnamomum cassia Blume, and plant essential oils extracted from Lauraceae Cinnamomum zeylanicum Nees. , Microcapsules composition for controlling mushroom flies. The method according to any one of claims 5 to 7, wherein the mushroom fly is Lycoriella mali Fitch), Blackwing Mushroom Fly ( Porodonta) flavipes Meigen), and small root flies ( Bradysia agrestis Sasakawa) is at least one member selected from the group consisting of, microcapsules for controlling mushroom flies. A mushroom fly control product comprising the mushroom fly control composition of any one of claims 1 to 3 or the microcapsule composition for mushroom fly control of any one of claims 5 to 7. The method of claim 9, wherein the mushroom fly is Lycoriella mali Fitch), Blackwing Mushroom Fly ( Porodonta) flavipes Meigen, and small root flies ( Bradysia) agrestis Sasakawa) one or more species selected from the group consisting of mushroom fly control products. Claims 1 to 3, wherein any one of the mushroom fly control composition or any one of claims 5 to 7 of the mushroom fly control microcapsule composition comprising the step of applying to the mushroom, mushroom fly control method. The method of claim 11, wherein the mushroom is one or more selected from the group consisting of shiitake mushroom, oyster mushroom, situation mushroom, enoki mushroom, matsutake mushroom, mushroom mushroom, and matsutake mushroom.

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