CN103783093B - A kind of have sterilization, the compositions of preventing and treating nematicide effect - Google Patents
A kind of have sterilization, the compositions of preventing and treating nematicide effect Download PDFInfo
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- CN103783093B CN103783093B CN201410090706.1A CN201410090706A CN103783093B CN 103783093 B CN103783093 B CN 103783093B CN 201410090706 A CN201410090706 A CN 201410090706A CN 103783093 B CN103783093 B CN 103783093B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a kind of have sterilization, preventing and treating nematicide effect compositions, raw material including following percentage by weight: vegetable oil 1 60%, antioxidant 0.1 0.5%, surfactant 10 30%, surplus is low VOC solvent, it solves vegetable oil and can not be applied to the technical problem of soil, provide a kind of compositions with sterilization and nematicide dual function, it does not contains toxicity, environmentally safe, belongs to environmental type preparation.Vegetable oil and other component are mixed and made into liquid preparation by the present invention; it is easy to be applied to soil sterilization and nematicide; the effective ingredient to the present composition that adds of antioxidant serves the effect of protection; surfactant makes the vegetable oil emulsion that dispersed formation is stable in water, and solvent plays diluting effect.
Description
Technical field
The present invention relates to a kind of compositions, particularly to a kind of, there is sterilization, the compositions of preventing and treating nematicide effect, belong to agricultural pest prevention and control field.
Background technology
The disease of the five disaster preventing and treatings that Zhi Bing circle generally believes is soil-borne disease, vascular bundle diseases, virus disease, bacteriosis and nematodiasis, and soil-borne disease extensively, is seriously listed in first of the disease of this five disaster preventing and treating due to harm.The preventing and treating of soil-borne disease has been passed by very long course, from the application development of the soil sterilants of killing livestock property such as chloropicrin, bromomethane and various sterilization agent to Biological control, but the propagation of soil-borne disease is still advocated rampant with harm, this is to be worth us to ponder deeply the problem with further investigated.
The root of soil-borne disease main harm crop and stem, once there is disease in plant growth early stage, seedling root rot or stem rot are rotten, and seedling will soon be dead, has a strong impact on plant growth.Plant growth deuterogenesis's disease, general time underproduction 20%-30%, serious time underproduction 50%-60%, even have no harvest, after soil-borne disease occurs, relatively difficult preventing and treating, pathogenic bacteria is ensconced in soil and survives the winter, is difficult to be killed, continuing infringement crop the coming year, so circulate, disease is increasingly severe.
Soil nematodes and disease have substantial connection.Soil nematodes can cause the wound of root system of plant, favourable infection process and make disease increase the weight of, often nematicide occurs with fungal disease simultaneously, as inseparable with soil nematodes in cotton wilt, is referred to as withered nematicide in american cotton droop and is combined disease.
Nematosis in the many plants including crops and forestry (Bursaphelenchus xylophilus), the loss annual about 100,000,000,000 dollars according to estimates that the whole world causes to agricultural production because of eelworm harm and the compound disease that therefore causes.Particularly in greenhouse and booth, Plant nematode life span is long, and density is big, brings serious harm to garden crop.The most how to control and eliminate the harm of Plant nematode to have caused in worldwide and pay attention to widely.Main prevention and controls or the chemical prevention particularly high-toxic pesticide of plant root-knot nematode is prevented and treated at present, and such as Aldicarb, Furadan, bromomethane, chloropicrin, metham-sodium etc., wherein Furadan a lot of provinces at home are the most disabled；Bromomethane is disabled in developed country in 2005, it is contemplated that within 2015, will be disabled in developing country comprehensively；Chloropicrin is disabled on a lot of crops such as Chinese cabbage etc..These high poison chemical pesticides, directly pass through in fruit or water, air arrival people, animal body, or are ultimately transferred to people, animal by environment, food chain；Additionally, owing to soil pollution has disguise, latency and chronicity, it constitutes serious harm by vegeto-animal food chain to human health, thus be difficult to be discovered by people, as the acute toxicity Oral Administration in Rats LD50 of Aldicarb be 0.65 mg/kg of Mouse oral LD50 be 0.30 mg/kg.Avilamycin has good eelworm-killing activity, especially B2a composition, but its formulation products containing dimethylbenzene, DMF and ketopyrrolidine contour poison organic solvent is applied to agricultural, causes the biggest pollution while preventing and treating nematicide.
Under System of Modern Agriculture, using in a large number of chemical pesticide of severe toxicity brings serious problem to agricultural production, food safety and environmental conservation etc..Therefore, a kind of effective, environmentally friendly, easy-to-use novel formulation of society need, for sustainable agriculture service.
Summary of the invention
The purpose of the present invention is that for above-mentioned the deficiencies in the prior art, it is provided that a kind of safe and environment-friendly sterilization, the compositions of preventing and treating nematicide, it does not contains toxicity, environmentally safe, belongs to environmental type preparation.
To achieve these goals, the technical solution used in the present invention is such that
A kind of have sterilization, the compositions of preventing and treating nematicide effect, and including the raw material of following percentage by weight: vegetable oil 1-60%, antioxidant 0.1-0.5%, surfactant 10-30%, surplus is low VOC solvent.
Above-mentioned vegetable oil is one or more in Oleum Cinnamomi, eucalyptus oil, Oleum Caryophylli.
Above-mentioned low VOC solvent is one or more in rapeseed oil, soybean oil methyl ester, turpentine oil, methyl oleate.
Above-mentioned antioxidant is 2,6-toluene di-tert-butyl phenol (BHT), tert-butyl hydroquinone (TBHQ), Butylated hydroxyanisole (BHA), eugenol, Moschus grass meal, one or more in Rhizoma et radix valerianae phenol.
Above-mentioned surfactant is agriculture breast 500# and Nongru-700 #.
Eucalyptus oil, mainly comprises: 1, 8-Cineole (more than 80%), camphene, phellandrene, terpineol, geranyl alcohol, isovaleral citronellal and piperitone etc., for colourless or slightly yellow liquid.In peculiar refrigerant Folium eucalypti globueli (Eucalyptus globulus Labill.) fragrance and band somewhat Camphora abnormal smells from the patient, carrying a little medicine gas, have the refrigerant sense of peppery mouth, fragrance is strong and lasting.The purposes eucalyptus oil of eucalyptus oil is by purposes can medicinal oil, spice be oily and iundustrial oil three kinds.Being Eucalyptus Globulus oil pharmaceutically applying more, Eucalyptus Globulus oil has the effect of anti-various bacteria, particularly to upper respiratory tract infection, chronic bronchitis, has phlegm-dispelling functions.Clinically by bacteriostatic experiment, find that Eucalyptus globulus Labill leaf oil has bacteriostasis, when instilling two methods application by sucking with trachea, treatment pulmonary tuberculosis patient is had gratifying effect.It is preferred like relatively other anti-scarring agent on common sympton, especially void closing eliminating.Eucalyptus Globulus oil can also be used for some dermatosis, and as wound face, ulcer, the irrigation of fistula, deodorizer and neuropathic analgesic.In addition Eucalyptus Globulus oil is also commonly used for rheocamphoradin, menthocamphorate, essential balm, mosquitofugal oil, antitussive etc..Eucalyptus citriodora oil uses in spice and daily chemical products, and eucalyptus citriodora oil contains citronellal, can be used for menthol-dl, thymol essence, also can isolate rhodinol, Fructus Citri Limoniae alcohol from eucalyptus citriodora oil, be used for preparing perfume, fancy soap, essential oil, prickly-heat powder etc..Industrial eucalyptus oil can be used for flotation ore in sand form, additive in detergents, surfactant and antibacterial.Eucalyptus oil is a kind of natural detergent, if the kind fabric of usual clothes, carpet has chewing gum to be difficult to wash off, but recently it has been found that make detergent with eucalyptus oil and be very easy to clean this type of dirt mark.Further investigation finds again, and eucalyptus oil has higher surface activity and a sterilizing ability, and abundance, nuisanceless generation, a kind of very good naturally occurring emulsifying agent and antibacterial.
Oleum Cinnamomi is the extracted volatile oil obtained of bark, leaves or branch that canella Cortex Cinnamomi is dried, and has Xin Lie and warms up sweet fragrance, and band is burnt, wood, cream are fragrant.Containing the most important functional component in Oleum Cinnamomi, wherein more than 80% is cinnamic aldehyde, additionally contain the composition of cinnamyl alcohol, cinnamic acid, coumarin, acetic acid Cortex cinnamomi japonici (Ramulus Cinnamomi) ester, 1-Phenylethanone. etc. more than tens kinds, can be as natural spice, antioxidant, antibacterial and preservative.Therefore, can be used widely in industries such as food, medicine, chemical industry.
Oleum Caryophylli, colourless or pale yellow liquid, there is strong Flos Caryophylli fragrance, water insoluble.It is mainly used in antibacterial, blood pressure lowering: can also be used in perfume, essence and various cosmetic essence and fragrance for detergents formula, it is also possible to for the allotment of edible essence.
In the present invention use vegetable oil be Oleum Cinnamomi, eucalyptus oil, Oleum Caryophylli one or more, there is sterilization and the effect of preventing and treating nematicide, they are used widely in industries such as food, medicine, chemical industry, and its toxicity is low, clearly indicate at U.S. EPA 40 CFR 152:25B and avoid registration.Although the vegetable oil that the present invention uses is acknowledged as having sterilization and nematicide activity, but, their high volatility and low-solubility limit they uses in soil, and therefore vegetable oil and other component are mixed and made into liquid preparation by the present invention, it is simple to be applied to soil sterilization and nematicide.The effective ingredient to the present composition that adds of antioxidant serves the effect of protection, and surfactant makes the vegetable oil emulsion that dispersed formation is stable in water, and solvent plays diluting effect.And the solvent that the present invention uses is low VOC solvent, to ecological environment non-hazardous.
Therefore, the present invention solves vegetable oil and can not be applied to the technical problem of soil, it is provided that a kind of compositions with sterilization and nematicide dual function, and it does not contains toxicity, environmentally safe, belongs to environmental type preparation.
Detailed description of the invention
In order to be more clearly understood from the purpose of the present invention, technical scheme and beneficial effect, below the present invention is described further, but not by protection scope of the present invention limit in the examples below.
The present invention is a kind of has sterilization, the compositions of preventing and treating nematicide effect, and including the raw material of following percentage by weight: vegetable oil 1-60%, antioxidant 0.1-0.5%, surfactant 10-30%, surplus is low VOC solvent.
Above-mentioned vegetable oil is one or more in Oleum Cinnamomi, eucalyptus oil, Oleum Caryophylli.
Above-mentioned low VOC solvent is one or more in rapeseed oil, soybean oil methyl ester, turpentine oil, methyl oleate.
Above-mentioned antioxidant is 2,6-toluene di-tert-butyl phenol (BHT), tert-butyl hydroquinone (TBHQ), Butylated hydroxyanisole (BHA), eugenol, Moschus grass meal, one or more in Rhizoma et radix valerianae phenol.
Above-mentioned surfactant is agriculture breast 500# and Nongru-700 #.
The compositions of the present invention, priority agents group is as follows: (by weight percentage)
Test with above-mentioned preparation:
One, LC50 and LC90 of in vitro root-knot nematode J2
1, test objective: measure LC50 and LC90 to root-knot nematode J2.
2, experimental condition
2.1 for examination target: root-knot nematode Meloidogyne incognica
2.2 instrument and equipments: the graceful funnel of shellfish, organize pulper, sieve, count plates, magnetic stirrer, liquid-transfering gun, centrifuge, suction pipe, high-pressure nozzle, anatomical lens, nematicide hordeolum.
3, test method
Take 1 ml root-knot nematode second instar larvae suspension, containing root-knot nematode second instar larvae 30-50 bar, detect J2 under stereomicroscope and live borer population, add medicament, observe residue nematicide when to make nematicide and medicament time of contact be 30min and live borer population.
4, result of the test
Two, LC50 and LC90 in vitro Bursaphelenchus xylophilus
1, test objective: measure LC50 and LC90 to Bursaphelenchus xylophilus.
2, experimental condition
2.1 for examination target: Bursaphelenchus xylophilus Bursaphelenchus xylophilus
2.2 instrument and equipments: number culture dish, magnetic stirrer, liquid-transfering gun, centrifuge, anatomical lens, nematicide hordeolum.
3, test method
Taking Bursaphelenchus xylophilus nematicide suspension, often process the 40-60 bar Han nematicide, under stereomicroscope, detection is lived borer population, adds medicament, makes nematicide and medicament time of contact observe residue nematicide borer population alive when being 0.5 h and 1 h.
4, result of the test
Three, the test of pesticide effectiveness potted plant to root-knot nematode egg and second instar larvae
1, test objective:
To root-knot nematode egg and two larva preventive effects, filter out optimal medicament and spraying medicine concentration, and kill line activity with commercially available medicament 1.8% avilamycin (dimethylbenzene) and compare.
2, experimental condition
2.1 for examination target with for studying thing
Tomato plants (3-4 leaf), Meloidogyne incognita (Meloidogyne incognica), comparison medicament: 1.8% abamectin emulsifiable concentrate
2.2 condition of culture
The soil texture (sand: soil=2:1), greenhouse temperature (15-34 DEG C)
3, EXPERIMENTAL DESIGN
(1) dress basin: take a diameter of 30cm plastic flowerpot and fill soil (every basin soil homogeneous band has line eggs and two larvas), and inject 400ml water to keep ground moistening；
(2) pretreatment (1 time): before transplanting, by each medicament dilute in proportion, the 1st perfusion, every basin perfusion amount is 200 ml, and each process repeats for 3 times；
(3) transplant: after pretreatment 10 days, transplant tomato seedling (young plant size is uniform), and final singling of watering；
(4) post processing (2 times): after transplanting 2 days, the 2nd perfusion (treatment agent is consistent with pretreatment), every basin perfusion amount is 200 ml；After the 2nd perfusion 10 days, carry out the 3rd perfusion (treatment agent is consistent with pretreatment).
(5) record data: after 2 months, button basin results plant, measure plant height and carry out disease scale and take pictures, plant above ground portion is cut gently, recording each process each repeatedly top and the fresh weight of root.According to blank incidence, root eelworm harm situation is carried out classification investigation, uses following grade scale:
0 grade: without root knot
1 grade: root knot area accounts for less than the 25% of whole area
2 grades: root knot area accounts for the 26-50% of whole area
3 grades: root knot area accounts for the 51-75% of whole area
4 grades: root knot area accounts for more than the 75% of whole area
4, test data
Four, to root-knot nematode egg and the potted plant test of pesticide effectiveness of second instar larvae
1, test objective: measure line eggs and two larva preventive effects
2, experimental condition
2.1 for examination target with for studying thing:
Tomato plants (3-4 leaf), Meloidogyne incognita (Meloidogyne incognica)
2.2 condition of culture
The soil texture (sand: soil=2:1), greenhouse temperature (18-34 DEG C)
3, EXPERIMENTAL DESIGN
Taking a diameter of 6 cm nutritive cubes on October 6 and fill 150 ml soil, inoculum concentration is 2000 root-knot nematode eggs and second instar larvae.Carrying out following pretreatment: October 7 processed for the first time, October 14, second time processed, and every basin formulation rate is 40 ml, and October 21 transplanted tomato plants (4-5 leaf).Button basin results plant, measured plant height and carried out disease scale November 12.Plant above ground portion is cut gently, records each process each repeatedly top and the fresh weight of root.
4, test data
Five, the indoor biometrics of Fusarium oxysporum is tested
1, test objective
The test indoor bactericidal activity to Fusarium oxysporum. Fusarium oxysporum (Fusarium.oxysporum) is a kind of universal soil-borne disease fungal pathogens, host range is extensive, can cause the generation of the 100 various plants droops such as melon, Solanaceae, Fructus Musae, cotton, pulse family and flowers.
2, test method and step: use auxin rate algoscopy
2.1 prepare band poison culture medium (each group all configures 50ml)
To be diluted to a series of concentration for reagent product final concentration with sterile distilled water according to each for reagent liquid, the medicinal liquid the most accurately drawing 0.5ml joins the 50ml culture medium dissolved.(culture medium is cooled to about 55 DEG C and adds) mix homogeneously, obtains the band poison culture medium of ultimate density, pours the culture dish of sterilizing into, and each gradient sets four repetitions, cooling the solid culture base plane of band poison.
2.2 inoculation pathogen
Take band bacterium culture medium post with the card punch of 0.5cm, be then put in band poison culture medium plane with Inoculating needle picking bacterium cake.Each culture dish puts a bacterium cake in centre, and mycelia faces up.
2.3 cultivate
The culture medium inoculated is placed in constant incubator cultivation, temperature 28 DEG C, but owing to indoor temperature reduces, actual cultivation temperature is between 26 ~ 27 DEG C.
2.4 result inspection and method for expressing thereof
72h after pure culture biscuits involvng inoculation, takes out culture dish and measures colony diameter (representing with mm).Each bacterium colony decussation surveys 2 diameters, represents bacterium colony size with its mean.Substitute into formula obtain suppression pathogen growth rate:
Developing restraint rate=[1-(processes colony diameter-bacterium cake diameter)/(comparison colony diameter-bacterium cake diameter)] 100%
3, result of the test
Above example illustrates, sterilization and the nematicide effect of the present composition are notable.
Claims (2)
1. one kind has sterilization, the compositions of preventing and treating nematicide effect, it is characterised in that: said composition includes
The raw material of following percentage by weight: vegetable oil 1-60%, antioxidant 0.1-0.5%, surfactant 10-30%,
Surplus is low VOC solvent；Described vegetable oil is selected from Oleum Cinnamomi, eucalyptus oil and Oleum Caryophylli；
Described low VOC solvent is selected from rapeseed oil, soybean oil methyl ester, turpentine oil and methyl oleate；
Described surfactant is selected from agriculture breast 500# and Nongru-700 #.
The most according to claim 1 a kind of have sterilization, the compositions of preventing and treating nematicide effect, and it is special
Levy and be: described antioxidant is selected from 2, and 6-toluene di-tert-butyl phenol (BHT), the tert-butyl group is to benzene two
Phenol (TBHQ), Butylated hydroxyanisole (BHA), eugenol, Moschus grass meal and Rhizoma et radix valerianae phenol.
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| CN115161240B (en) * | 2022-07-25 | 2023-10-24 | 四川龙蟒福生科技有限责任公司 | Bacillus velezensis strain and application thereof |
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