CN113796394A - Mixed use method of biological preparation containing eucalyptus oil and biological insecticide - Google Patents

Mixed use method of biological preparation containing eucalyptus oil and biological insecticide Download PDF

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Publication number
CN113796394A
CN113796394A CN202111238768.9A CN202111238768A CN113796394A CN 113796394 A CN113796394 A CN 113796394A CN 202111238768 A CN202111238768 A CN 202111238768A CN 113796394 A CN113796394 A CN 113796394A
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eucalyptus oil
parts
biological
preparation containing
weight
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Inventor
任启均
曹亭
杨廉勇
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Yibin Zhangzhimei Biotechnology Co ltd
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Yibin Zhangzhimei Biotechnology Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00Protecting plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/06Aluminium; Calcium; Magnesium; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/24Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/28Myrtaceae [Myrtle family], e.g. teatree or clove
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a biological compound preparation containing eucalyptus oil and a preparation method thereof, wherein the biological compound preparation comprises 1-100 parts by weight of eucalyptus oil and 1-100 parts by weight of cyanazine. The biological compound preparation containing the eucalyptus oil has good effect of preventing and treating root nematodes, low toxicity and environmental protection.

Description

Mixed use method of biological preparation containing eucalyptus oil and biological insecticide
Technical Field
The invention relates to the field of cinnamomum camphora application, and particularly relates to a biological compound preparation containing eucalyptus oil and a using method thereof.
Background
Cinnamomum camphora (with the academic name of Gamble N. Chao exH. W. Li) is a evergreen arbor of Lauraceae, with height of 20 m and diameter at breast height of 50 cm; the bark is gray, the branch is cylindrical, hairless, the young branch is fine, the leaves are intergrowth, oval or elliptical, the tip is suddenly short and gradually sharp to long and gradually sharp, the upper surface is dark green, bright, the lower surface is grey green, dark, the two surfaces are hairless, the leaf stalk is flat and back convex, light green, the cone inflorescence axillary, is fine, the branch is fine and weak, each branch is 3-7 flowers of the polypodium, the shaft is hairless, the flower is light yellow, fragrance is provided, the stalk is fine, the flower quilt is cone-shaped, the flower quilt is oval, the filament is white and soft, the filament is glandless, the anther is long round, the ovary is oval, the young fruit is spherical, the flower is in 5-6 months, and the fruit is yielded in 7-9 months.
Yibin is the primary resource of the cinnamomum camphora, and by the end of 2018, 47 ten thousand mu of cinnamomum camphora forest base in the whole market has the crude cinnamomum camphora oil yield of over 70 percent of the whole country, the yibin crude cinnamomum camphora has high oil yield and good oil quality, and belongs to a non-inspection product in the international market.
At present, the main methods for extracting the crude camphor oil comprise a CO2 supercritical extraction method, a water vapor extraction method, an extraction method and the like, and target products are extracted from the crude camphor oil through the extraction methods, wherein the eucalyptus oil is one of the target products.
Root nematodes, also known as root knot nematodes, are an insect of the phylum zoophylum, class nematoda. Male and female variant. The larvae are in the shape of slender worms, harm vegetables, have wide host range and can survive for one year under the condition of no host. The overground part of the damaged plant grows short and slow, the color of the leaves is abnormal, the fruiting is less, the yield is low, and even the plant dies in advance.
Most root nematodes live in soil, are parasitic in plants, are transmitted through soil or seeds, can damage root systems of the plants or invade aerial organs, affect the growth and development of crops, indirectly transmit diseases caused by other microorganisms, and cause great economic loss in agriculture. At present, the special nematicides with better effects in the world are few, only more than ten kinds, and the existing nematicides with better effects have higher toxicity to people and livestock.
Lime nitrogen is a mixture of calcium cyanamide, calcium oxide and other insoluble impurities, is gray black, has special odor, and can be used as root nematode pesticide with a spreading amount of 30g/m2The control effect is about 50 percent.
The existing eucalyptus oil is generally used as cough drops, gum candies, gargles, toothpaste and air cleaning agents, and other purposes are not reported.
Disclosure of Invention
Based on the problems, on one hand, the invention provides a biological compound preparation containing eucalyptus oil, which has the advantages of good effect of preventing and treating root nematodes, low toxicity and environmental protection.
A biological compound preparation containing eucalyptus oil comprises:
1-100 parts of eucalyptus oil and 1-100 parts of cyanazine.
In one or more specific embodiments herein, the eucalyptus oil is 10 to 20 parts by weight, and the lime nitrogen is 10 to 20 parts by weight.
In one or more specific embodiments of the present application, the eucalyptus oil is prepared from sassafras.
In one aspect, the invention also provides a method for using the biological compound preparation containing the eucalyptus oil.
The application method of the biological compound preparation containing the eucalyptus oil comprises the step of spreading the biological compound preparation containing the eucalyptus oil in soil, wherein the spreading amount is 10-100g/m2
In one or more specific embodiments of the present application, the broadcast amount is 30g/m2
In one or more specific embodiments of the present application, the eucalyptus oil-containing biocomposite formulation is used for controlling and/or killing root nematodes of plants.
In one or more specific embodiments of the present application, the plant is cucumber.
The invention principle and the beneficial effects of the application are as follows:
according to the invention, the lime nitrogen, the eucalyptus oil and the polyethylene glycol are mixed to be used as the biological compound preparation, and surprisingly, the root nematode control agent has a good effect, is low in toxicity and is environment-friendly.
Detailed Description
The present invention will be further explained below.
The examples provided herein are merely to further illustrate the invention and should not be construed as limiting the invention in any way.
It will be apparent to those skilled in the art that the materials and methods of operation used in the present invention are well known in the art, unless otherwise specified, in the following.
In the present invention, parts by weight means measured in units of weight, such as 1 part by weight means: when the weight is Kg, 1 part by weight means 1 Kg; when the weight is g, 1 part by weight means 1g, and the like, and is not limited to a specific Kg or g.
In examples 1-3 below, cyanamide (hymexazol), polyethylene glycol and eucalyptus oil, which is prepared by extracting crude camphor oil, are commercially available.
Example 1
Taking 15 parts by weight of lime nitrogen and 15 parts by weight of eucalyptus oil, and uniformly mixing to obtain a sample 1.
Example 2
10 parts by weight of cyanamide and 20 parts by weight of eucalyptus oil were mixed uniformly to prepare sample 2.
Example 3
20 parts by weight of cyanamide and 10 parts by weight of eucalyptus oil were mixed uniformly to obtain sample 3.
Example 4
30 parts by weight of lime nitrogen was taken as sample 4.
Example 5
30 parts by weight of eucalyptus oil was used as sample 5.
Example 6
Samples 1-5 and 36 planting pots with uniform size and planting soil are taken.
Sample 1 is group 1, sample 2 is group 2, sample 3 is group 3, sample 4 is group 4, and sample 5 is group 5.
36 planting basins that are equipped with planting soil that size is homogeneous divide into 6 groups, and sample 1 corresponds 6 and plants the basin, and sample 2 corresponds 6 and plants the basin, and sample 3 corresponds 6 and plants the basin, and sample 4 corresponds 6 and plants the basin, and sample 5 corresponds 6 and plants the basin, and remaining 6 are planted the basin and correspond the contrast group.
The same number of cucumber seeds were sown in each pot, with group 1 treated with sample 1, group 2 treated with sample 2, group 3 treated with sample 3, group 4 treated with sample 4, group 5 treated with sample 5 and the control group untreated. The treatment of groups 1-5 is broadcast application with a broadcast application amount of 30g/m2
After 7 days of sowing, root nematodes were inoculated in two holes on the left and right sides of the cucumber seedlings. After 10 days of inoculation, the upper plants were cut off and the planting soil was removed from the roots. The root nematodes are stained with acid fuchsin staining solution, the root nematodes are observed and quantified under a 40-fold dissecting mirror, and the control effect of each group is determined according to the observed and quantified results, which are shown in table 1 below.
TABLE 1
Figure BDA0003318450580000041
Figure BDA0003318450580000051
In table 1, the control effect calculation is based on the number of root nematodes in the control group, and the calculation formula is:
control group root nematode number-per group root nematode number)/control group root nematode number
The number of the root nematodes in the control group is the average number of the root nematodes in the control group, and the average number of the root nematodes in the control group is the average value calculated after removing suspicious data by the Grubbs criterion.
The number of the root nematodes in the control group is the average number of the root nematodes in the control group, and the number of the root nematodes in each group is the average value calculated after removing the suspicious data by the Grubbs criterion.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. A biological compound preparation containing eucalyptus oil comprises:
1-100 parts of eucalyptus oil and 1-100 parts of cyanazine.
2. The biological compound preparation containing eucalyptus oil according to claim 1, wherein the eucalyptus oil is 10 to 20 parts by weight, and the cyanamide is 10 to 20 parts by weight.
3. The bio-composite preparation containing eucalyptus oil according to claim 1 or 2, wherein the eucalyptus oil is prepared from sassafras oil.
4. A method for using the eucalyptus oil-containing biocomposite preparation according to any one of claims 1 to 3, wherein the eucalyptus oil-containing biocomposite preparation according to any one of claims 1 to 3 is applied to soil in an amount of 10 to 100g/m2
5. The use method of the bio-composite preparation containing eucalyptus oil according to claim 4, wherein the amount of application is 30g/m2
6. The use method of the eucalyptus oil-containing biological compound preparation according to any one of claims 4 to 6, wherein the eucalyptus oil-containing biological compound preparation is used for controlling and/or killing root nematodes of plants.
7. The use method of the bio-complex formulation containing eucalyptus oil according to claim 6, wherein the plant is cucumber.
CN202111238768.9A 2021-10-25 2021-10-25 Mixed use method of biological preparation containing eucalyptus oil and biological insecticide Pending CN113796394A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1732773A (en) * 2005-07-15 2006-02-15 中国农业科学院蔬菜花卉研究所 A kind of soil disinfection method of facility for prevention and control soil-borne diseases of vegetable
CN103783093A (en) * 2014-03-13 2014-05-14 四川龙蟒福生科技有限责任公司 Composition having functions of sterilization and nematode prevention and control
US20150125435A1 (en) * 2013-11-01 2015-05-07 Thomas T. Yamashita Compositions for Controlling Plant Parasitic Nematodes and Methods for Using Same
CN107986869A (en) * 2017-12-13 2018-05-04 江西省农业科学院农产品质量安全与标准研究所 A kind of medicine fertilizer and its application process and purposes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1732773A (en) * 2005-07-15 2006-02-15 中国农业科学院蔬菜花卉研究所 A kind of soil disinfection method of facility for prevention and control soil-borne diseases of vegetable
US20150125435A1 (en) * 2013-11-01 2015-05-07 Thomas T. Yamashita Compositions for Controlling Plant Parasitic Nematodes and Methods for Using Same
CN103783093A (en) * 2014-03-13 2014-05-14 四川龙蟒福生科技有限责任公司 Composition having functions of sterilization and nematode prevention and control
CN107986869A (en) * 2017-12-13 2018-05-04 江西省农业科学院农产品质量安全与标准研究所 A kind of medicine fertilizer and its application process and purposes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
曹齐卫 等: "《绿色蔬菜高效生产关键技术丛书 黄瓜绿色高效生产关键技术》", vol. 1, 30 June 2015, 山东科学技术出版社, pages: 92 - 94 *
赵善欢 等: "《植物质杀虫剂成分及作用机理(讲义)》", 30 September 1995, 华南农业大学植保系昆虫毒理研究室, pages: 84 - 85 *
马俊峰: "《除草剂使用技术》", vol. 1, 31 August 2018, 中原农民出版社, pages: 326 - 327 *

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